Bhi06G000334 (gene) Wax gourd (B227) v1

Overview


Name	Bhi06G000334
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Ribonuclease DdI-like
Location	chr6: 8746763 .. 8749234 (-)
RNA-Seq Expression	Bhi06G000334
Synteny	Bhi06G000334

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGAATATAACGTTTTGAAATTATCTTAAAAGAAACACATCTTAATTATCTTATTAGAAGATATGTTGATGTCTAAGAAGAAAAGTTTATTTCTTCTCCTATGCATTTTGTTGTTGAATCTTTTAATAGGGGATGCTTTAGATGAGTTAGAAGATGATGATATAAAAAATGAGGATGCCTTCAATGAGCTAAAGTATGAACTAGCTTTCATGGAATCAAAAAATAATGATCATAGTTTCTATGATTTGGAGGAGATGAAAGCAGTTCCATTTGATTATTATCAACTTGTATTGCAATGGCAACCAGCAACTTGCAGCAATGTCATATGTCTAAGACCCACGTCGTCGAGGTTTAGCATCGACGGTCTTTGGGCTGGCAGCTATTCTCGTCCTATTGGTCGTTGCTCTGGCAGCAAATTTCTTCAACAAAACGTATGCGTTCTATTATGACTTAGAGTCTTGCACCTTTTAAATATATTTTTACTATAATGTGGTATTAATTTTAAGTTTACTTCATAACTAGCTATTGGGTTAATATCTTCAACATCATCCCATACCTAGCTAGAATAAAATTTTGGTTCTTCATACATCTATTTTCTAGCATACTCCCCTTCAAGGAATCAACAAATAAATTATTCAAGAACCTCCACTAAAAATAAATTAGAGAAAAAAAACCCCTAAGATCCTTCCCAACTTAATATTTCTATGGGTGTATTTTATGTCATCTTCAATAAAGCTCTATTATTCCTATTAAATTCCATGTGGTAAAAAGAGTCCAATCCAATACTTCATGATTACTAAACACCATTTTTTTTAAGTTATTAAATAAAACATTTGGTTCAATTGTTTTAATGTCTTGTTTATGATCGTTAGCAAAACAATCTCAAAGTTTTTATTTTTTAAAATTTAAAAGTCAATATAAAAAAAATATAGAGTTGATAGTTCTTGATCAACCTTGTGTTGTAAAAAAAATCCAAAAATTAGTTCAAATCGCTTCAATATATTGGATAAACATGAAGTTACATCTCAATATGTGAACTTTAACCTATTTTCTTAGAATGCGAATAGAGTTGATTTCAAGAATGTTAAAAGTGATTAAGAAAGAATCTTACTTATTTTTTTGTGCTTTATGATTAAATCCATTAAATTTTATTTCATATAGTTTATAAGATCAGTTTTCTATTAGCTTGTTTTATCCTTCAAAATTAATATTTATTTTATATCTCTAGTAATTTTTTCACCTAACCAAAATCTATCTTCTATTTTTTCTTAGGTTGAAAAGAACCATTTTTTTTATAAAAAAAAATCATACCATATTATTTTTTTTTAAAAAAAAGAAATTAAACCCTTTTCTCCTACTACTTAACAGCTAATCTAAAACAAACAAAATATAACATTATAAACAATCATTTAAAAATTATAAAGAAAAATACAAATCGTAGGTTTATTTTAGTTATTATATAATTTAGTATTTATTTACCAAACACGATAACCAAACTTATTCAGTACTAAAGCTAAAATATACCCAAACATTAACTTGCTTGCAATTATTTTATAAGTCATTGAAATCCCAAAGTTAGTCTAATATGGTACATTTCTAGATTCAACATTCTTAGATTAGATCTTTTTTCTTATTTGGATTTCAGAAGCCTTTCGACACCATATTAGATAATCATAAATTAAAACGAATGAGAAGAATAACCCGAGTTATTTTTATCTTTGAGAGATCAATCTCTTTTATTTTTTCAATATAAAACCTTCAACATAATCATATGACTGAACTCTAAAAAGATTGGATCATTTGGTGTGTAATAATTTGGAAACAGATCACAAGCATAAGAAAAGAACTAGATGAGGATTGGCCAAGTTTGATAATAAGCTCAAACCCAGCAGTATGGAGTGAAGCATGGAATTTACGAGGCACATGTTTCGAAACCCCAACGTTCCAAATCGTCGATTACTTCCGATTAGCTCTTTACCTTTTCTGGAGAAGCGACGTGCAGCAAGCCCTTCAAAAATCTGGGCTGGAACCCATCAATGGGAAGCGCTATCTAAAGTCCGACATTGAAACTGCCCTAACAAAAAGCTTTGGAAAGCCGGCTCTGCGCTGCACCTTGAACTTAAAGTACCTTCTTCAGTCTCAGTTGGCGGAAGTGGTTCTTTGCTTTGATAAATGTCTTCGAAACATTGACTGTCCTTCTCAATATTCTGCTGCCACTGTGTGTCCTACACGAATCCTGTGGCTCAAAACATAGTCATTATCTCTCATGCAAAGGGATAAATTTACATAAATAAACAAAGTTCTTCATTCCCTTTGCCTCTTTTGTGTGTTGGGAGTTGGGTGATGATTATATATGTATGTATCATCTCATCATCAATAAATTAGTCATCATATCTATCCTTTGCTTTACCAACTTTCATGTTTATATGGTATATATATTAAGGTTAGGCTATTATTAAAAAAAGTGGGTA

mRNA sequence

ATAGAATATAACGTTTTGAAATTATCTTAAAAGAAACACATCTTAATTATCTTATTAGAAGATATGTTGATGTCTAAGAAGAAAAGTTTATTTCTTCTCCTATGCATTTTGTTGTTGAATCTTTTAATAGGGGATGCTTTAGATGAGTTAGAAGATGATGATATAAAAAATGAGGATGCCTTCAATGAGCTAAAGTATGAACTAGCTTTCATGGAATCAAAAAATAATGATCATAGTTTCTATGATTTGGAGGAGATGAAAGCAGTTCCATTTGATTATTATCAACTTGTATTGCAATGGCAACCAGCAACTTGCAGCAATGTCATATGTCTAAGACCCACGTCGTCGAGGTTTAGCATCGACGGTCTTTGGGCTGGCAGCTATTCTCGTCCTATTGGTCGTTGCTCTGGCAGCAAATTTCTTCAACAAAACATCACAAGCATAAGAAAAGAACTAGATGAGGATTGGCCAAGTTTGATAATAAGCTCAAACCCAGCAGTATGGAGTGAAGCATGGAATTTACGAGGCACATGTTTCGAAACCCCAACGTTCCAAATCGTCGATTACTTCCGATTAGCTCTTTACCTTTTCTGGAGAAGCGACGTGCAGCAAGCCCTTCAAAAATCTGGGCTGGAACCCATCAATGGGAAGCGCTATCTAAAGTCCGACATTGAAACTGCCCTAACAAAAAGCTTTGGAAAGCCGGCTCTGCGCTGCACCTTGAACTTAAAGTACCTTCTTCAGTCTCAGTTGGCGGAAGTGGTTCTTTGCTTTGATAAATGTCTTCGAAACATTGACTGTCCTTCTCAATATTCTGCTGCCACTGTGTGTCCTACACGAATCCTGTGGCTCAAAACATAGTCATTATCTCTCATGCAAAGGGATAAATTTACATAAATAAACAAAGTTCTTCATTCCCTTTGCCTCTTTTGTGTGTTGGGAGTTGGGTGATGATTATATATGTATGTATCATCTCATCATCAATAAATTAGTCATCATATCTATCCTTTGCTTTACCAACTTTCATGTTTATATGGTATATATATTAAGGTTAGGCTATTATTAAAAAAAGTGGGTA

Coding sequence (CDS)

ATGTTGATGTCTAAGAAGAAAAGTTTATTTCTTCTCCTATGCATTTTGTTGTTGAATCTTTTAATAGGGGATGCTTTAGATGAGTTAGAAGATGATGATATAAAAAATGAGGATGCCTTCAATGAGCTAAAGTATGAACTAGCTTTCATGGAATCAAAAAATAATGATCATAGTTTCTATGATTTGGAGGAGATGAAAGCAGTTCCATTTGATTATTATCAACTTGTATTGCAATGGCAACCAGCAACTTGCAGCAATGTCATATGTCTAAGACCCACGTCGTCGAGGTTTAGCATCGACGGTCTTTGGGCTGGCAGCTATTCTCGTCCTATTGGTCGTTGCTCTGGCAGCAAATTTCTTCAACAAAACATCACAAGCATAAGAAAAGAACTAGATGAGGATTGGCCAAGTTTGATAATAAGCTCAAACCCAGCAGTATGGAGTGAAGCATGGAATTTACGAGGCACATGTTTCGAAACCCCAACGTTCCAAATCGTCGATTACTTCCGATTAGCTCTTTACCTTTTCTGGAGAAGCGACGTGCAGCAAGCCCTTCAAAAATCTGGGCTGGAACCCATCAATGGGAAGCGCTATCTAAAGTCCGACATTGAAACTGCCCTAACAAAAAGCTTTGGAAAGCCGGCTCTGCGCTGCACCTTGAACTTAAAGTACCTTCTTCAGTCTCAGTTGGCGGAAGTGGTTCTTTGCTTTGATAAATGTCTTCGAAACATTGACTGTCCTTCTCAATATTCTGCTGCCACTGTGTGTCCTACACGAATCCTGTGGCTCAAAACATAG

Protein sequence

MLMSKKKSLFLLLCILLLNLLIGDALDELEDDDIKNEDAFNELKYELAFMESKNNDHSFYDLEEMKAVPFDYYQLVLQWQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQNITSIRKELDEDWPSLIISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQQALQKSGLEPINGKRYLKSDIETALTKSFGKPALRCTLNLKYLLQSQLAEVVLCFDKCLRNIDCPSQYSAATVCPTRILWLKT

Homology

[Close]

BLAST of Bhi06G000334 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-15
Identity = 64/206 (31.07%), Postives = 96/206 (46.60%), Query Frame = 0


Query: 70  FDYYQLVLQWQPATC-SNVICLRPTSSR----FSIDGLWA--GSYSRPIGRCSGSKFLQQ 129
           FD++  VLQW  A C ++  C  PTS +    F I GLW      S P      S+F + 
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFDRS 87

Query: 130 NITSIRKELDEDWPSLIISSNPA--VWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSD 189
            I+ +   L ++WP+L   SN     W   W   GTC E+   Q  +YF  AL L  +++
Sbjct: 88  QISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQ-HEYFENALKLKQKAN 147

Query: 190 VQQALQKSGLEPINGKRYLKSDIETALTKSFG-KPALRCTLNLKYLLQSQLAEVVLCFDK 249
           + Q L+ SG+ P +G  Y    I  A+    G  P + C  N      +QL ++ +C D 
Sbjct: 148 LLQILKNSGINPDDG-FYNLDKITNAIKDGIGFTPGIEC--NKDPERNAQLHQIYICVDT 207

Query: 250 C-LRNIDCPSQYSAATVCPTRILWLK 265
                I+CP     +  CP++I + K
Sbjct: 208 SGTEFIECPVLPRGS--CPSQIQFSK 227

[Close]

BLAST of Bhi06G000334 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.8e-16
Identity = 58/173 (33.53%), Postives = 85/173 (49.13%), Query Frame = 0


Query: 70  FDYYQLVLQWQPATCS---NVICLRPTSSRFSIDGLWAGSYSRPIGRC-SGSKFLQQNIT 129
           FD +  V QW PA CS   +  C       F+I GLW       +  C  GS F    I+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 130 SIRKELDEDWPSLIISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQQAL 189
            ++ +L+  WP+++ ++N   WS  W   GTC E+ TF    YF+LA+ +    D+  AL
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSES-TFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 190 QKSGLEPINGKRYLKSDIETALTKSFGK-PALRCTLNLKYLLQSQLAEVVLCF 238
           +     P NG+   +  I+  L   FGK P LRC  + +  + S L +VV CF
Sbjct: 121 RPHAAGP-NGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKV-SYLVQVVACF 170

[Close]

BLAST of Bhi06G000334 vs. ExPASy Swiss-Prot
Match: Q40381 (Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.9e-15
Identity = 55/186 (29.57%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 70  FDYYQLVLQWQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQNITSIRK 129
           F+Y QLVLQW  A C    C R   + F+I GLW  + S  +  C+  +  +      +K
Sbjct: 24  FEYMQLVLQWPTAFCHTTPCKR-IPNNFTIHGLWPDNVSTTLNYCAAKENFKNIEDDTKK 83

Query: 130 -ELDEDWPSLIIS-----SNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQQ 189
            +L + WP L  +      +   W   +N  G C  + ++    YF LA+ L  + D+  
Sbjct: 84  DDLYKRWPDLTTAETYCKQHQNFWRHEYNKHGKCC-SESYNREQYFDLAMALKDKFDLLS 143

Query: 190 ALQKSGLEPINGKRYLKSDIETALTK-SFGKPALRCTLNLKYLLQSQLAEVVLCFDKCLR 248
           +L+  G+ P  G +Y    I + + K + G P L CT  +      +L E+ +CFD  ++
Sbjct: 144 SLRNHGIIPGRGMKYTVQKINSTIKKITQGYPNLSCTKGI-----MELVEIGICFDSMVK 202

[Close]

BLAST of Bhi06G000334 vs. ExPASy Swiss-Prot
Match: Q40379 (Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 1.0e-12
Identity = 62/201 (30.85%), Postives = 87/201 (43.28%), Query Frame = 0


Query: 70  FDYYQLVLQWQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQNITSIRK 129
           F+Y QLVLQW  A C    C +   S F+I GLW  + S  +  C         +    K
Sbjct: 24  FEYMQLVLQWPTAFCHTTPC-KNIPSNFTIHGLWPDNVSTTLNFCGKEDDYNIIMDGPEK 83

Query: 130 E-LDEDWPSLIISSNPAV-----WSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQQ 189
             L   WP LI      +     W   +   GTC  +  +  V YFRLA+ L  + D+  
Sbjct: 84  NGLYVRWPDLIREKADCMKTQNFWRREYIKHGTCC-SEIYNQVQYFRLAMALKDKFDLLT 143

Query: 190 ALQKSGLEPINGKRYLKSDI-ETALTKSFGKPALRCTLNLKYLLQSQLAEVVLCFDKCLR 249
           +L+  G+  I G +Y    I  T  T + G P L CT         +L  V +CFD   +
Sbjct: 144 SLKNHGI--IRGYKYTVQKINNTIKTVTKGYPNLSCTKG------QELWFVGICFDSTAK 203

Query: 250 N-IDCPSQYSAATVCPTRILW 263
           N IDCP+  +  T     I++
Sbjct: 204 NVIDCPNPKTCKTASNQGIMF 214

[Close]

BLAST of Bhi06G000334 vs. ExPASy Swiss-Prot
Match: P10281 (Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 5.8e-08
Identity = 41/146 (28.08%), Postives = 63/146 (43.15%), Query Frame = 0


Query: 94  SSRFSIDGLWAGSYSRPIGRCSGSKFLQQNITSIRKE---------LDEDWPSLIISSNP 153
           S  ++I GLW  +     G+         NIT+I +E         + + WP+     + 
Sbjct: 64  SDSWTIHGLWPDNCDGSYGQFCDKSREYSNITAILQEQGRTELLSYMKKYWPN-YEGDDE 123

Query: 154 AVWSEAWNLRGTCFET----------PTFQIVDYFRLALYLFWRSDVQQALQKSGLEPIN 213
             W   WN  GTC  T          P  ++ DY +  + LF   D  +AL K+G+ P +
Sbjct: 124 EFWEHEWNKHGTCINTIEPSCYKDYSPQKEVGDYLQKTVDLFKGLDSYKALAKAGIVPDS 183

Query: 214 GKRYLKSDIETALTKSFG--KPALRC 219
            K Y +S+IE+AL       KP + C
Sbjct: 184 SKTYKRSEIESALAAIHDGKKPYISC 208

[Close]

BLAST of Bhi06G000334 vs. ExPASy Swiss-Prot
Match: Q5AKB1 (Ribonuclease T2-like 1-B OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=RNY1-B PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.5e-05
Identity = 29/87 (33.33%), Postives = 44/87 (50.57%), Query Frame = 0


Query: 147 WSEAWNLRGTCF----------ETPTFQ-IVDYFRLALYLFWRSDVQQALQKSGLEPING 206
           W+  +N  GTCF           TP  Q + D+FR+A+ LF +      L ++G+ P N 
Sbjct: 144 WAHEYNKHGTCFSTIKPSCYLSNTPKNQNLYDFFRIAIGLFNKLPTYDWLAEAGIVPTNS 203

Query: 207 KRYLKSDIETALTKSFGKPA-LRCTLN 222
           K Y  S+I++AL   FG    ++C  N
Sbjct: 204 KTYSLSEIQSALNDKFGANVFIKCDYN 230

[Close]

BLAST of Bhi06G000334 vs. ExPASy TrEMBL
Match: A0A0A0KS79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636520 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 7.4e-107
Identity = 191/249 (76.71%), Postives = 214/249 (85.94%), Query Frame = 0


Query: 17  LLNLLIGDALDELEDDDIKNEDAFNELKYELAFMESKNNDHSFYDLEEMKAVPFDYYQLV 76
           +LNLLIGDAL+ LE++              +AF ESKN+  SFY LE MK VPFDYYQLV
Sbjct: 1   MLNLLIGDALNGLEEN--------------IAFTESKNDHPSFYGLERMKEVPFDYYQLV 60

Query: 77  LQWQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQNITSIRKELDEDWP 136
           LQWQPATCSN ICLRP SSRFSI+GLWA SYSRPIGRC+G+ FLQQNITSIRKELDEDWP
Sbjct: 61  LQWQPATCSNAICLRPWSSRFSINGLWAASYSRPIGRCTGNGFLQQNITSIRKELDEDWP 120

Query: 137 SLIISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQQALQKSGLEPINGK 196
           SL+IS+NPAVWSEAWNL+GTCFE+PTFQI DYFRLALYLFWRSDVQ+ALQ+SG+EPINGK
Sbjct: 121 SLVISANPAVWSEAWNLQGTCFESPTFQINDYFRLALYLFWRSDVQKALQESGIEPINGK 180

Query: 197 RYLKSDIETALTKSFGKPALRCTLNLKYLLQSQLAEVVLCFDKCLRNIDCPSQYSAATVC 256
           +Y KSDIE A+TKSFGKPALRC LNLKYLLQSQL++V LCFDKCL +IDCPS+YS A  C
Sbjct: 181 QYEKSDIEAAITKSFGKPALRCNLNLKYLLQSQLSQVFLCFDKCLAHIDCPSKYSPALGC 235

Query: 257 PTRILWLKT 266
           PT+ILW KT
Sbjct: 241 PTKILWNKT 235

[Close]

BLAST of Bhi06G000334 vs. ExPASy TrEMBL
Match: A0A5A7TE29 (Ribonuclease DdI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00270 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 5.0e-103
Identity = 186/247 (75.30%), Postives = 206/247 (83.40%), Query Frame = 0


Query: 19  NLLIGDALDELEDDDIKNEDAFNELKYELAFMESKNNDHSFYDLEEMKAVPFDYYQLVLQ 78
           NLLIGDAL+ELE+D              + F+E KN+ HSF DLE MK +PFDYYQLVLQ
Sbjct: 3   NLLIGDALNELEED--------------IDFLEPKNDHHSFNDLEGMKEIPFDYYQLVLQ 62

Query: 79  WQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQNITSIRKELDEDWPSL 138
           WQPATCS  ICLRP SSRFSI+GLWAGSYSR IGRC GS+FL+QN+TSIRKELD+DWPSL
Sbjct: 63  WQPATCSAAICLRPFSSRFSINGLWAGSYSRLIGRCIGSRFLEQNVTSIRKELDKDWPSL 122

Query: 139 IISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQQALQKSGLEPINGKRY 198
           IISSNPAVWSEAWNL GTCFETPTFQI DYFRLALYLFWRSDVQ+ALQ  G+EPINGK+Y
Sbjct: 123 IISSNPAVWSEAWNLHGTCFETPTFQINDYFRLALYLFWRSDVQKALQDFGIEPINGKQY 182

Query: 199 LKSDIETALTKSFGKPALRCTLNLKYLLQSQLAEVVLCFDKCLRNIDCPSQYSAATVCPT 258
            KS IE A+T+ FGKPALRC L+ KY LQSQLA+VVLCF+KCL  IDCPSQYS A  CPT
Sbjct: 183 EKSYIEAAITEKFGKPALRCNLSGKYFLQSQLAQVVLCFNKCLARIDCPSQYSPALGCPT 235

Query: 259 RILWLKT 266
           +ILWLKT
Sbjct: 243 KILWLKT 235

[Close]

BLAST of Bhi06G000334 vs. ExPASy TrEMBL
Match: A0A6J1JWY7 (ribonuclease DdI-like OS=Cucurbita maxima OX=3661 GN=LOC111489590 PE=3 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 4.5e-88
Identity = 169/256 (66.02%), Postives = 194/256 (75.78%), Query Frame = 0


Query: 3   MSKKKSLFLLLCILLLNLLIGDALDELEDDDIKNEDAFNELKYELAFMESKNNDHSFYDL 62
           M+ K + FLLL IL+LN+L G                     Y +A  +S N      +L
Sbjct: 1   MANKNTFFLLLTILILNVLKG---------------------YAMASNDSSN------EL 60

Query: 63  EEMKAVPFDYYQLVLQWQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQ 122
           EEMKAVPFDYYQLVLQWQPATCSN+ CL+P SSRFSI+GLWAGSYS  IGRCSGS+FLQQ
Sbjct: 61  EEMKAVPFDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYSGSIGRCSGSEFLQQ 120

Query: 123 NITSIRKELDEDWPSL-IISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDV 182
           NI+SIR+E DEDWPSL +I SNPAVW E W+L GTCFETPTFQI DYFRLALYLFW+SDV
Sbjct: 121 NISSIRREQDEDWPSLVVIGSNPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDV 180

Query: 183 QQALQKSGLEPINGKRYLKSDIETALTKSFGKPALRCTLNLKYLLQSQLAEVVLCFDKCL 242
            QAL+K G+EP +G+RYL  D E+ L  SFGKPALRC+ N+KY LQ QLAEV LCFDKCL
Sbjct: 181 LQALEKYGMEPADGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCL 229

Query: 243 RNIDCPSQYSAATVCP 258
           R+I+CPSQYSAA  CP
Sbjct: 241 RHINCPSQYSAANGCP 229

[Close]

BLAST of Bhi06G000334 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 5.2e-28
Identity = 74/185 (40.00%), Postives = 107/185 (57.84%), Query Frame = 0


Query: 70  FDYYQLVLQWQPATCSN----VICLRPTSSRFSIDGLWAGSYSRPIGRCSGS-KFLQQNI 129
           FDYYQ+VLQWQPATCS+     +C++P   R+S+ G+W    S  +  CSG+  +  + I
Sbjct: 25  FDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARMI 84

Query: 130 TSIRKELDEDWPSLIISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQQA 189
            +I+  LD+DWP++I   NP  WS  W   GTC      Q   YF   L+++   D+Q  
Sbjct: 85  LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQF-HYFNFGLWVYTNYDLQSI 144

Query: 190 LQKSGLEPINGKRYLKSDIETALTKSFGK-PALRCTLNLKYLLQSQLAEVVLCFDKCLR- 248
           L + G+ P NG++Y K+ ++ A+  + GK PALRC  N +     QL E+ LCF K  + 
Sbjct: 145 LYQYGIYP-NGQQYTKTVVQNAIYSATGKWPALRCNNNYR-TGNMQLHEISLCFSKNGQI 204

[Close]

BLAST of Bhi06G000334 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 6.8e-28
Identity = 82/220 (37.27%), Postives = 111/220 (50.45%), Query Frame = 0


Query: 50  MESKNNDHSFYDLEEMKAVPFDYYQLVLQWQPATCSN----VICLRPTSSRFSIDGLWAG 109
           ME K     F  L  + +  ++YYQ+ LQWQPATCS+      C+     RFSI G+W  
Sbjct: 1   MEKKGIVLVFSTLVVIASCDYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHGVWPT 60

Query: 110 SYSRPIGRCSGS-KFLQQNITSIRKELDEDWPSLIISSNPAVWSEAWNLRGTCFETPTFQ 169
             S    +C G+  +    I++IR  L+ DWPS+I  +NP +WS  W   GTC   P+  
Sbjct: 61  LSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTC-SYPSLS 120

Query: 170 IVDYFRLALYLFWRSDVQQALQKSGLEPINGKRYLKSDIETALTKSFGK-PALRCTLNLK 229
            +DYF   L  + + D+  AL K  + P +   Y KS I  A+ K   K P LRC  N  
Sbjct: 121 QLDYFTFGLDTYAKYDIYSALDKEDIYP-SEDVYSKSSINNAIRKITQKLPGLRCNTN-D 180

Query: 230 YLLQSQLAEVVLCFDKCLRN-IDCPSQYSAATVCPTRILW 263
           Y  + QL E++LCF K  R  IDCPS       C   + W
Sbjct: 181 YSRERQLHEIILCFAKSNRAIIDCPSN---VVTCGNYVYW 214

The following BLAST results are available for this feature:

BLAST of Bhi06G000334 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	1.3e-15	31.07	Ribonuclease T2 family protein	[more]

BLAST of Bhi06G000334 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	5.8e-16	33.53	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
Q40381	2.9e-15	29.57	Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1	[more]
Q40379	1.0e-12	30.85	Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2	[more]
P10281	5.8e-08	28.08	Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=...	[more]
Q5AKB1	3.5e-05	33.33	Ribonuclease T2-like 1-B OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=...	[more]

BLAST of Bhi06G000334 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS79	7.4e-107	76.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636520 PE=3 SV=1	[more]
A0A5A7TE29	5.0e-103	75.30	Ribonuclease DdI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold89...	[more]
A0A6J1JWY7	4.5e-88	66.02	ribonuclease DdI-like OS=Cucurbita maxima OX=3661 GN=LOC111489590 PE=3 SV=1	[more]
A0A6J1E0X1	5.2e-28	40.00	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1E266	6.8e-28	37.27	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 68..263 e-value: 2.9E-38 score: 134.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 68..262
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 70..246 e-value: 2.5E-30 score: 106.0
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 65..260
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 65..260

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000334	Bhi06M000334	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding