Bhi06G000333 (gene) Wax gourd (B227) v1

Overview


Name	Bhi06G000333
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	ATP-dependent zinc metalloprotease FTSH 6, chloroplastic
Location	chr6: 8745028 .. 8745306 (+)
RNA-Seq Expression	Bhi06G000333
Synteny	Bhi06G000333

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGACAATGTTTGGGATGTCAGAGATAAGGCCATGGACATTGATAGATCCAATAGTACAGAGCAGTGATGTGGTGATGAGAACGTTAGCTCGAAACTCCATGTCAGAGAAGTTAGCAGAAGACATCGATTCATCCATACGAAATATAATAGAAAATGCATACGAAGCCATTGACAAATTGGTAGATGTTTTGTTGGAGAATAAACAATCTCAGGAGATGAATTTAGATCTATTCTTTCGAAGTTCACTAACATTCCCTCAGACAACGAAATCCTAA

mRNA sequence

ATGGTGACAATGTTTGGGATGTCAGAGATAAGGCCATGGACATTGATAGATCCAATAGTACAGAGCAGTGATGTGGTGATGAGAACGTTAGCTCGAAACTCCATGTCAGAGAAGTTAGCAGAAGACATCGATTCATCCATACGAAATATAATAGAAAATGCATACGAAGCCATTGACAAATTGGTAGATGTTTTGTTGGAGAATAAACAATCTCAGGAGATGAATTTAGATCTATTCTTTCGAAGTTCACTAACATTCCCTCAGACAACGAAATCCTAA

Coding sequence (CDS)

ATGGTGACAATGTTTGGGATGTCAGAGATAAGGCCATGGACATTGATAGATCCAATAGTACAGAGCAGTGATGTGGTGATGAGAACGTTAGCTCGAAACTCCATGTCAGAGAAGTTAGCAGAAGACATCGATTCATCCATACGAAATATAATAGAAAATGCATACGAAGCCATTGACAAATTGGTAGATGTTTTGTTGGAGAATAAACAATCTCAGGAGATGAATTTAGATCTATTCTTTCGAAGTTCACTAACATTCCCTCAGACAACGAAATCCTAA

Protein sequence

MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAYEAIDKLVDVLLENKQSQEMNLDLFFRSSLTFPQTTKS

Homology

[Close]

BLAST of Bhi06G000333 vs. TAIR 10
Match: AT5G15250.1 (FTSH protease 6 )

HSP 1 Score: 93.2 bits (230), Expect = 1.2e-19
Identity = 52/80 (65.00%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVTMFGMSEI PW L DP V+ +DVV+R LARNSMSEKLAEDIDS ++ II +AY     
Sbjct: 574 MVTMFGMSEIGPWALTDPAVKQNDVVLRMLARNSMSEKLAEDIDSCVKKIIGDAYEVAKK 633

Query: 61  ------EAIDKLVDVLLENK 70
                 EAIDKLVDVLLE +
Sbjct: 634 HVRNNREAIDKLVDVLLEKE 653

[Close]

BLAST of Bhi06G000333 vs. TAIR 10
Match: AT5G15250.2 (FTSH protease 6 )

HSP 1 Score: 93.2 bits (230), Expect = 1.2e-19
Identity = 52/80 (65.00%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVTMFGMSEI PW L DP V+ +DVV+R LARNSMSEKLAEDIDS ++ II +AY     
Sbjct: 595 MVTMFGMSEIGPWALTDPAVKQNDVVLRMLARNSMSEKLAEDIDSCVKKIIGDAYEVAKK 654

Query: 61  ------EAIDKLVDVLLENK 70
                 EAIDKLVDVLLE +
Sbjct: 655 HVRNNREAIDKLVDVLLEKE 674

[Close]

BLAST of Bhi06G000333 vs. TAIR 10
Match: AT2G30950.1 (FtsH extracellular protease family )

HSP 1 Score: 80.5 bits (197), Expect = 7.8e-16
Identity = 45/80 (56.25%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVT FGMS+I PW+L+D   Q SDV+MR +ARNSMSEKLAEDIDS+++ + ++AY     
Sbjct: 577 MVTTFGMSDIGPWSLMDSSAQ-SDVIMRMMARNSMSEKLAEDIDSAVKKLSDSAYEIALS 636

Query: 61  ------EAIDKLVDVLLENK 70
                 EA+DKLV+VLLE +
Sbjct: 637 HIKNNREAMDKLVEVLLEKE 655

[Close]

BLAST of Bhi06G000333 vs. TAIR 10
Match: AT1G06430.1 (FTSH protease 8 )

HSP 1 Score: 76.3 bits (186), Expect = 1.5e-14
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVT FGMSEI PW+L+D   Q SDV+MR +ARNSMSEKLA DID++++ + + AY     
Sbjct: 570 MVTTFGMSEIGPWSLMDSSEQ-SDVIMRMMARNSMSEKLANDIDTAVKTLSDKAYEIALS 629

Query: 61  ------EAIDKLVDVLLENK 70
                 EA+DK+V++LLE +
Sbjct: 630 QIRNNREAMDKIVEILLEKE 648

[Close]

BLAST of Bhi06G000333 vs. ExPASy Swiss-Prot
Match: Q1PDW5 (ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSH6 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.6e-18
Identity = 52/80 (65.00%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVTMFGMSEI PW L DP V+ +DVV+R LARNSMSEKLAEDIDS ++ II +AY     
Sbjct: 574 MVTMFGMSEIGPWALTDPAVKQNDVVLRMLARNSMSEKLAEDIDSCVKKIIGDAYEVAKK 633

Query: 61  ------EAIDKLVDVLLENK 70
                 EAIDKLVDVLLE +
Sbjct: 634 HVRNNREAIDKLVDVLLEKE 653

[Close]

BLAST of Bhi06G000333 vs. ExPASy Swiss-Prot
Match: Q67WJ2 (ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FTSH6 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 2.2e-15
Identity = 44/80 (55.00%), Postives = 56/80 (70.00%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAYE---- 60
           MVT FGMSEI PW L +P  Q  DVV+R LAR+SMSE+LA DID+++R I++ AYE    
Sbjct: 573 MVTAFGMSEIGPWALAEPAAQGGDVVLRMLARSSMSERLAADIDAAVRTIVDEAYEVAKA 632

Query: 61  -------AIDKLVDVLLENK 70
                  AID+LVDVL+E +
Sbjct: 633 HVRRNRAAIDQLVDVLMEKE 652

[Close]

BLAST of Bhi06G000333 vs. ExPASy Swiss-Prot
Match: O80860 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSH2 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.1e-14
Identity = 45/80 (56.25%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVT FGMS+I PW+L+D   Q SDV+MR +ARNSMSEKLAEDIDS+++ + ++AY     
Sbjct: 577 MVTTFGMSDIGPWSLMDSSAQ-SDVIMRMMARNSMSEKLAEDIDSAVKKLSDSAYEIALS 636

Query: 61  ------EAIDKLVDVLLENK 70
                 EA+DKLV+VLLE +
Sbjct: 637 HIKNNREAMDKLVEVLLEKE 655

[Close]

BLAST of Bhi06G000333 vs. ExPASy Swiss-Prot
Match: Q655S1 (ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FTSH2 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.4e-14
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MV  FGMS+I PW+L+D   QS DV+MR +ARNSMSEKLAEDID++++ + + AY     
Sbjct: 564 MVVTFGMSDIGPWSLMDSGAQSGDVIMRMMARNSMSEKLAEDIDTAVKRLSDEAYEIALS 623

Query: 61  ------EAIDKLVDVLLENK 70
                 EA+DK+V+VLLE +
Sbjct: 624 QIRSNREAMDKIVEVLLEKE 643

[Close]

BLAST of Bhi06G000333 vs. ExPASy Swiss-Prot
Match: Q8W585 (ATP-dependent zinc metalloprotease FTSH 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSH8 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 2.1e-13
Identity = 42/80 (52.50%), Postives = 57/80 (71.25%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVT FGMSEI PW+L+D   Q SDV+MR +ARNSMSEKLA DID++++ + + AY     
Sbjct: 570 MVTTFGMSEIGPWSLMDSSEQ-SDVIMRMMARNSMSEKLANDIDTAVKTLSDKAYEIALS 629

Query: 61  ------EAIDKLVDVLLENK 70
                 EA+DK+V++LLE +
Sbjct: 630 QIRNNREAMDKIVEILLEKE 648

[Close]

BLAST of Bhi06G000333 vs. ExPASy TrEMBL
Match: A0A5A7T9F3 (ATP-dependent zinc metalloprotease FTSH 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00260 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.8e-20
Identity = 62/80 (77.50%), Postives = 63/80 (78.75%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVT FGMSEI PWTLIDP VQSSDVVMR LARNSMSEKLAEDIDSSIRNIIE AY     
Sbjct: 565 MVTTFGMSEIGPWTLIDPAVQSSDVVMRMLARNSMSEKLAEDIDSSIRNIIEKAYEIAKT 624

Query: 61  ------EAIDKLVDVLLENK 70
                 EAIDKLVDVLLE +
Sbjct: 625 HIRNNREAIDKLVDVLLEKE 644

[Close]

BLAST of Bhi06G000333 vs. ExPASy TrEMBL
Match: A0A1S3CAE3 (ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498485 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.8e-20
Identity = 62/80 (77.50%), Postives = 63/80 (78.75%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAY----- 60
           MVT FGMSEI PWTLIDP VQSSDVVMR LARNSMSEKLAEDIDSSIRNIIE AY     
Sbjct: 565 MVTTFGMSEIGPWTLIDPAVQSSDVVMRMLARNSMSEKLAEDIDSSIRNIIEKAYEIAKT 624

Query: 61  ------EAIDKLVDVLLENK 70
                 EAIDKLVDVLLE +
Sbjct: 625 HIRNNREAIDKLVDVLLEKE 644

[Close]

BLAST of Bhi06G000333 vs. ExPASy TrEMBL
Match: A0A6A1VIC4 (Ribonuclease T(2) OS=Morella rubra OX=262757 GN=CJ030_MR6G013928 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-19
Identity = 58/80 (72.50%), Postives = 62/80 (77.50%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAYE---- 60
           MVTMFGMSEI PW L DP VQS DVV+R LARNSMSEKLAEDIDSS+RN+IENAYE    
Sbjct: 425 MVTMFGMSEIGPWALTDPAVQSGDVVLRMLARNSMSEKLAEDIDSSVRNVIENAYEVAKD 484

Query: 61  -------AIDKLVDVLLENK 70
                  AIDKLVDVLLE +
Sbjct: 485 HIRNNRDAIDKLVDVLLEKE 504

[Close]

BLAST of Bhi06G000333 vs. ExPASy TrEMBL
Match: G7L1Y9 (ATP-dependent zinc metalloprotease FTSH protein OS=Medicago truncatula OX=3880 GN=11414171 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-19
Identity = 58/80 (72.50%), Postives = 64/80 (80.00%), Query Frame = 0


Query: 1   MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAYE---- 60
           MVT +GMSEI PWTLIDP VQSSDVV+R LARNSMSEKLAEDID+S+R+IIE AYE    
Sbjct: 559 MVTTYGMSEIGPWTLIDPSVQSSDVVLRMLARNSMSEKLAEDIDNSVRHIIETAYEVAKN 618

Query: 61  -------AIDKLVDVLLENK 70
                  AIDKLVDVLLEN+
Sbjct: 619 HIKNNRDAIDKLVDVLLENE 638

[Close]

BLAST of Bhi06G000333 vs. ExPASy TrEMBL
Match: A2Q1U2 (Peptidase M41 OS=Medicago truncatula OX=3880 GN=MtrDRAFT_AC149128g28v2 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-19
Identity = 58/80 (72.50%), Postives = 64/80 (80.00%), Query Frame = 0


Query: 1  MVTMFGMSEIRPWTLIDPIVQSSDVVMRTLARNSMSEKLAEDIDSSIRNIIENAYE---- 60
          MVT +GMSEI PWTLIDP VQSSDVV+R LARNSMSEKLAEDID+S+R+IIE AYE    
Sbjct: 16 MVTTYGMSEIGPWTLIDPSVQSSDVVLRMLARNSMSEKLAEDIDNSVRHIIETAYEVAKN 75

Query: 61 -------AIDKLVDVLLENK 70
                 AIDKLVDVLLEN+
Sbjct: 76 HIKNNRDAIDKLVDVLLENE 95

The following BLAST results are available for this feature:

BLAST of Bhi06G000333 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15250.1	1.2e-19	65.00	FTSH protease 6	[more]
AT5G15250.2	1.2e-19	65.00	FTSH protease 6	[more]
AT2G30950.1	7.8e-16	56.25	FtsH extracellular protease family	[more]
AT1G06430.1	1.5e-14	52.50	FTSH protease 8	[more]

BLAST of Bhi06G000333 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1PDW5	1.6e-18	65.00	ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Arabidopsis thaliana...	[more]
Q67WJ2	2.2e-15	55.00	ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Oryza sativa subsp. ...	[more]
O80860	1.1e-14	56.25	ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Arabidopsis thaliana...	[more]
Q655S1	1.4e-14	52.50	ATP-dependent zinc metalloprotease FTSH 2, chloroplastic OS=Oryza sativa subsp. ...	[more]
Q8W585	2.1e-13	52.50	ATP-dependent zinc metalloprotease FTSH 8, chloroplastic OS=Arabidopsis thaliana...	[more]

BLAST of Bhi06G000333 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T9F3	1.8e-20	77.50	ATP-dependent zinc metalloprotease FTSH 6 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CAE3	1.8e-20	77.50	ATP-dependent zinc metalloprotease FTSH 6, chloroplastic OS=Cucumis melo OX=3656...	[more]
A0A6A1VIC4	1.2e-19	72.50	Ribonuclease T(2) OS=Morella rubra OX=262757 GN=CJ030_MR6G013928 PE=3 SV=1	[more]
G7L1Y9	2.6e-19	72.50	ATP-dependent zinc metalloprotease FTSH protein OS=Medicago truncatula OX=3880 G...	[more]
A2Q1U2	2.6e-19	72.50	Peptidase M41 OS=Medicago truncatula OX=3880 GN=MtrDRAFT_AC149128g28v2 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000642	Peptidase M41	PFAM	PF01434	Peptidase_M41	coord: 1..64 e-value: 2.7E-8 score: 33.9
IPR037219	Peptidase M41-like	GENE3D	1.20.58.760	Peptidase M41	coord: 1..81 e-value: 6.2E-6 score: 28.0
IPR037219	Peptidase M41-like	SUPERFAMILY	140990	FtsH protease domain-like	coord: 1..69
None	No IPR available	PANTHER	PTHR23076	METALLOPROTEASE M41 FTSH	coord: 1..72
None	No IPR available	PANTHER	PTHR23076:SF118	ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 6, CHLOROPLASTIC	coord: 1..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000333	Bhi06M000333	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006508	proteolysis
biological_process	GO:0016070	RNA metabolic process
cellular_component	GO:0009534	chloroplast thylakoid
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009526	plastid envelope
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004176	ATP-dependent peptidase activity
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding