Bhi06G000107 (gene) Wax gourd (B227) v1

Overview
NameBhi06G000107
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionexopolygalacturonase-like
Locationchr6: 2647504 .. 2647790 (-)
RNA-Seq ExpressionBhi06G000107
SyntenyBhi06G000107
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGGAAGGATGCTTGTGCATCAAAGATCAAAGTACAGTAATTATTCCAAACGCGGTTACAAGTTAAGTGCGATCCAGTTAGTAGGCCCTTGCAATAGTTCAATCGGAATTCAACTCCAAGGAACATTGCAAGCACCAATAGATCTGACAGGTGAAGATTGGGTTCATTTCAAATATATCGATCATTTTTCATTGACAAGTGGTGGAGTTTTTGAGGACAAGGAAAACAAGCTTGGGAAAGCAATGATTGTGATAAAAACCCTAATTGCGCAAGAATTCCCATT

mRNA sequence

ATGCATGGAAGGATGCTTGTGCATCAAAGATCAAAGTACAGTAATTATTCCAAACGCGGTTACAAGTTAAGTGCGATCCAGTTAGTAGGCCCTTGCAATAGTTCAATCGGAATTCAACTCCAAGGAACATTGCAAGCACCAATAGATCTGACAGGTGAAGATTGGGTTCATTTCAAATATATCGATCATTTTTCATTGACAAGTGGTGGAGTTTTTGAGGACAAGGAAAACAAGCTTGGGAAAGCAATGATTGTGATAAAAACCCTAATTGCGCAAGAATTCCCATT

Coding sequence (CDS)

ATGCATGGAAGGATGCTTGTGCATCAAAGATCAAAGTACAGTAATTATTCCAAACGCGGTTACAAGTTAAGTGCGATCCAGTTAGTAGGCCCTTGCAATAGTTCAATCGGAATTCAACTCCAAGGAACATTGCAAGCACCAATAGATCTGACAGGTGAAGATTGGGTTCATTTCAAATATATCGATCATTTTTCATTGACAAGTGGTGGAGTTTTTGAGGACAAGGAAAACAAGCTTGGGAAAGCAATGATTGTGATAAAAACCCTAATTGCGCAAGAATTCCCATT

Protein sequence

MHGRMLVHQRSKYSNYSKRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLTGEDWVHFKYIDHFSLTSGGVFEDKENKLGKAMIVIKTLIAQEFP
Homology
[Close]
BLAST of Bhi06G000107 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 2.8e-08
Identity = 30/79 (37.97%), Postives = 43/79 (54.43%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLTG-EDWVHFKYIDHFSLTSGGVFEDKE 77
           K  YKL  I ++GPC + I I L GT++A  +  G E WV F+ I+ F L  GGVF+ + 
Sbjct: 60  KGEYKLGEIVMMGPCKAPIRIALLGTVKADGNANGKEKWVAFRNINGFKLNGGGVFDGEG 119

Query: 78  NKLGKAMIVIKTLIAQEFP 96
           N   +     KT   ++ P
Sbjct: 120 NAAWRVNNCHKTFNCKKLP 138

[Close]
BLAST of Bhi06G000107 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 3.1e-07
Identity = 28/81 (34.57%), Postives = 42/81 (51.85%), Query Frame = 0 

Query: 17  SKRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLT--GEDWVHFKYIDHFSLTSGGVFED 76
           +K  +KL  I++ GPC + + I LQGTL+A        E WV F  I+ F L  GG+F+ 
Sbjct: 59  TKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLNGGGIFDG 118

Query: 77  KENKLGKAMIVIKTLIAQEFP 96
           + N   +     KT   ++ P
Sbjct: 119 EGNAAWRVNNCHKTFECKKLP 139

[Close]
BLAST of Bhi06G000107 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 6.9e-07
Identity = 28/80 (35.00%), Postives = 41/80 (51.25%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLT--GEDWVHFKYIDHFSLTSGGVFEDK 77
           K  +KL  I++ GPC + I + LQGT++A  +     E WV F  ID F L  GG F+ +
Sbjct: 59  KGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGE 118

Query: 78  ENKLGKAMIVIKTLIAQEFP 96
            N   +     KT   ++ P
Sbjct: 119 GNAAWRVNNCHKTFECKKLP 138

[Close]
BLAST of Bhi06G000107 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 49.3 bits (116), Expect = 2.0e-06
Identity = 21/56 (37.50%), Postives = 35/56 (62.50%), Query Frame = 0 

Query: 21  YKLSAIQLVGPCNSSIGIQLQGTLQAPID---LTGEDWVHFKYIDHFSLTSGGVFE 74
           + L  +   GPC SSI   ++GTL AP +   +  E+W+ FKY+D+ ++T GG+ +
Sbjct: 75  FYLRQVTFSGPCKSSITFFIRGTLLAPRNPYAINQEEWILFKYVDNLTVTGGGLLD 130

[Close]
BLAST of Bhi06G000107 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 9.9e-06
Identity = 27/80 (33.75%), Postives = 39/80 (48.75%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLT--GEDWVHFKYIDHFSLTSGGVFEDK 77
           K  +KL  I + GPC S + I L GT+ A  +     E WV F+ +D F L  GG F+ +
Sbjct: 60  KGQFKLGEIMMSGPCKSPVEITLLGTVLADGNSIHGKEKWVVFQRMDGFRLNGGGTFDGE 119

Query: 78  ENKLGKAMIVIKTLIAQEFP 96
            N   +     KT   ++ P
Sbjct: 120 GNAAWRVNNCHKTFECKKLP 139

[Close]
BLAST of Bhi06G000107 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-07
Identity = 27/59 (45.76%), Postives = 37/59 (62.71%), Query Frame = 0 

Query: 18 KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPID---LTGEDWVHFKYIDHFSLTSGGVFE 74
          K  + +  I L GPC SSIG+QLQGTL+AP D   + G  W++   ID  ++  GGVF+
Sbjct: 26 KGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKGLGWINLNKIDLLTIFGGGVFD 84

[Close]
BLAST of Bhi06G000107 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.8e-07
Identity = 25/57 (43.86%), Postives = 37/57 (64.91%), Query Frame = 0 

Query: 21  YKLSAIQLVGPCNSSIGIQLQGTLQAPIDLT----GEDWVHFKYIDHFSLTSGGVFE 74
           Y+L+ I+L GPC + I +Q+ GT+QAP D +     E W  F Y+DH +L+  GVF+
Sbjct: 65  YQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFD 121

[Close]
BLAST of Bhi06G000107 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.5e-06
Identity = 24/59 (40.68%), Postives = 35/59 (59.32%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPID---LTGEDWVHFKYIDHFSLTSGGVFE 74
           K  Y LS + L GPC + I I +QGT+QAP D       +WV F  +++F +  GG+F+
Sbjct: 69  KGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFD 127

[Close]
BLAST of Bhi06G000107 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.5e-06
Identity = 24/59 (40.68%), Postives = 35/59 (59.32%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPID---LTGEDWVHFKYIDHFSLTSGGVFE 74
           K  Y LS + L GPC + I I +QGT+QAP D       +WV F  +++F +  GG+F+
Sbjct: 69  KGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFD 127

[Close]
BLAST of Bhi06G000107 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 4.8e-05
Identity = 29/80 (36.25%), Postives = 40/80 (50.00%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLT-GED-WVHFKYIDHFSLTSGGVFEDK 77
           K  +KL    + GPC S I   LQG ++     T G+D WV F+ I+ F L  GG F+ +
Sbjct: 59  KGDFKLGETVMTGPCKSPIEFTLQGNVKTDGGSTQGKDRWVVFEKINGFKLNGGGTFDGE 118

Query: 78  ENKLGKAMIVIKTLIAQEFP 96
            N   KA    KT   ++ P
Sbjct: 119 GNAAWKANNCHKTFECKKLP 138

[Close]
BLAST of Bhi06G000107 vs. ExPASy TrEMBL
Match: A0A6J1DBN7 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.1e-15
Identity = 43/62 (69.35%), Postives = 49/62 (79.03%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLTGEDWVHFKYIDHFSLTSGGVFEDKEN 77
           K  Y+L+ + LVGPC S IGI+ QGTLQAPID TGE WV+FKYIDHFSLT GGVF D + 
Sbjct: 70  KASYQLTGVDLVGPCKSPIGIEFQGTLQAPIDPTGEGWVNFKYIDHFSLTGGGVF-DGQG 129

Query: 78  KL 80
           KL
Sbjct: 130 KL 130

[Close]
BLAST of Bhi06G000107 vs. ExPASy TrEMBL
Match: A0A6J1DUC6 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.6e-14
Identity = 38/56 (67.86%), Postives = 43/56 (76.79%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLTGEDWVHFKYIDHFSLTSGGVFE 74
           K  YKL  I L GPC S I +QLQGT+QAPIDLTGE WV FKYIDHF+L+ GG F+
Sbjct: 66  KVAYKLGTIDLTGPCKSPIEVQLQGTIQAPIDLTGEGWVQFKYIDHFTLSGGGTFD 121

[Close]
BLAST of Bhi06G000107 vs. ExPASy TrEMBL
Match: A0A5A7T7B3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001590 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 4.5e-14
Identity = 39/53 (73.58%), Postives = 44/53 (83.02%), Query Frame = 0 

Query: 21  YKLSAIQLVGPCNSSIGIQLQGTLQAPIDLTGEDWVHFKYIDHFSLTSGGVFE 74
           YKL  I+L G C S I IQ+QGTLQAP DLTGEDWVHFKYID+ S+TSGGVF+
Sbjct: 116 YKLGTIELKGECKSPIEIQVQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFD 168

[Close]
BLAST of Bhi06G000107 vs. ExPASy TrEMBL
Match: A0A1S3CC08 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 4.5e-14
Identity = 39/53 (73.58%), Postives = 44/53 (83.02%), Query Frame = 0 

Query: 21  YKLSAIQLVGPCNSSIGIQLQGTLQAPIDLTGEDWVHFKYIDHFSLTSGGVFE 74
           YKL  I+L G C S I IQ+QGTLQAP DLTGEDWVHFKYID+ S+TSGGVF+
Sbjct: 64  YKLGTIELKGECKSPIEIQVQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFD 116

[Close]
BLAST of Bhi06G000107 vs. ExPASy TrEMBL
Match: A0A6J1HJP4 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111465122 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.9e-11
Identity = 31/61 (50.82%), Postives = 42/61 (68.85%), Query Frame = 0 

Query: 18  KRGYKLSAIQLVGPCNSSIGIQLQGTLQAPIDLTGEDWVHFKYIDHFSLTSGGVFEDKEN 77
           K  Y L  I L+GPC S I I +QGT+ AP+D+TG+ W+ F+Y+D  SLT GGVF+ +  
Sbjct: 67  KETYMLGVITLLGPCKSPIDIVVQGTIMAPVDITGDGWIQFRYVDKISLTGGGVFDGQGQ 126

Query: 78  K 79
           K
Sbjct: 127 K 127

The following BLAST results are available for this feature:
BLAST of Bhi06G000107 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G48140.12.8e-0837.97Pectin lyase-like superfamily protein [more]
AT3G07840.13.1e-0734.57Pectin lyase-like superfamily protein [more]
AT3G07820.16.9e-0735.00Pectin lyase-like superfamily protein [more]
AT2G33160.12.0e-0637.50glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro... [more]
AT3G07830.19.9e-0633.75Pectin lyase-like superfamily protein [more]
BLAST of Bhi06G000107 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P245481.0e-0745.76Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q403121.8e-0743.86Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Q397661.5e-0640.68Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Q397861.5e-0640.68Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
P353374.8e-0536.25Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
BLAST of Bhi06G000107 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DBN71.1e-1569.35exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV... [more]
A0A6J1DUC62.6e-1467.86exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV... [more]
A0A5A7T7B34.5e-1473.58Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CC084.5e-1473.58exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1[more]
A0A6J1HJP47.9e-1150.82exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111465122 PE=3 SV=1[more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M000107Bhi06M000107mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity