Bhi06G000082 (gene) Wax gourd (B227) v1

Overview
NameBhi06G000082
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDNA-directed RNA polymerase II subunit 4-like
Locationchr6: 1925958 .. 1929879 (-)
RNA-Seq ExpressionBhi06G000082
SyntenyBhi06G000082
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATGGCAACCCTTCGTCCGGGTTGAAAACTAAGAAGCTGCTTCTCCTCTATCGCGCCCTTCTCTGTTTTCGCTACCTCTGATTTTGCCGCCGTCACGTCTCGCCGCCGCTGGATCTCGCCGTCGATCCTCTCTGTCTCCTGTGGTGCGTCCTTTCCTATCTCAGGTTTTCCTCTATTTACATACATTCTTCTAATTTCAGACGTCGGTTGAATCTTCAACATTAGAATTTGGCATTCATTTCCATTCTCCAAGATTACGAATCTATATTTTTTTTCTAATCTAGTAATACCTTTCGCCATTCTCAGTTAAAGTTTCACTGCTATTTAGGCGTTTAGTTACGGCTCAATTGCCCCTCTGTTCTAAAAAATTGCTGATTATTGTAGTTCATACAAACTTGATTTGGATGGAGTTTTTTGCCATTTAAATTAAAGGTTAAATGTTCCTAGGCTCATAAAGTGTATTACTAAATTCAAACCATGTTTGATTAATTGGAAACCTTGATAGAAATCACGAGACACAAGTGTTTATTCATGTATTTAATGAATTACATCATGAGGACAATTTTCTAATGTTATAGACTGAATCAAGTAAAAGTGAATGAAGGGATCTTTTGTGTCGTACCTTATTCAAAGTCTGATGTAGATTTAGTTTGATCCATTAGTGTAGTTCTTAAATCGTTTTTTAGATTTAGTTTTTATCTTGCTCTACTCGTTTCCAAATTTTGAAGGTTGGACAAATCTCTAACTTCAAGCATGTCTGGAGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGGTACGACAAATCTTTCTATAAATCTCTGATTTTCTATCAACATTCAATTCAATGAGATTTTAAGTAGCTCTTACTCTCCATGGCGCTGTGTTTCCTTCATGTATTTGAACATTTTGAATCTATTTAGCAATTCATGTATTTAATCTTTTAATTTGTTTAAAAAGTGCGAAACTCAATTTATTAAGTTGTTCTCCATGCCTAATCATATATGTATCCTTATTGCACATTCTCTGATCCTTCTAGTAGCCAATGTGGAGATTGTTTTTATGTTTTTAACAACGCATATAGCTTGGACTGAGAATGTGGAGTCAATGTAAACATTAGTTAATCTCAAAGAAATTTTGTGGGTAGAGTGGTAACTTAAAGAAATTAATTTTGCTAGAAATCAAGATTGAGAAGAGTGAAAGTGTATCAGCGCACACAAACAGGCACACAAAAGGAGTCCTCAATTAGAATGCAAAGAAGTTACAATCTCTTATATTGAATCACATACCTAGTCGATGGATGTTTTGCTTAGTCGAGAGAGGAGAAATATAGTAAACTTCCTTGTTCAAGGCCATTACTTTATGTAATTACGTCTTGTAGCCATCTACTTAGGATTGTAGAATCTCATAACTTTTTCAGATATTGTTAGGTAAGAAATAGTCATTAGATAGGTGGTTGGACATCCACGATTATGAAAAAAAGTTCCTTTTACGTGCACCTTCCATTTGTTGTTTATGATATCATGCTATTATTTTCCATAATGCATGTTCTTAATAGTATGAGCTGGTTCATTTACAAAATTGAAGCATCACTTTCTTAATGATGTGAAAACCTGGCCGATTGATCTAAAAAACCCTAGTCCTTGTGACATGATCATGACACCTCGTATCACTAAATATGAGTTTTTCTTTTTTTATATTTTGGATTGACATTATATGATTCTAAGGTTTTTGAAATTTAAGATGGAGATTTGTGATCTATGCAAAATCTTAATTCTAATCTATAATCATTTTGTCTATAAGATACTCAAACTATTGTAAGTTTTTCCAGGATAATGCAAGACTTGATTTATATGGATAAATAATTATTCAAATTTCTTTTTGACATCAAAATCTTTTTGGGAATACTCTTAAAGCTTTTTCATTCTCTTGTATTTATTTTGCTCTCTTTGCTCTTTGTTCTAAGGGCCATATATAAAGAGAAAATATAAAACGTGATAACTGACATTGCTTCCTTTGTCTGCCTGCAGAATTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAGTACGAGCAACTTCAACAAAGGGCTGAGGATCCAACGAATCAAGTTTCTCAGTGAGATTGTTTATTCTTTATTGATTCATAAGTTAAATTAGAGTTTCTCTTCTTTGTTGGCATAGCTTAAATATTTAGTTAAATTACGATATTCATTCTCACTTGCCATACAATAATGATGGATTTTTAATTTGGTATTCTTCTGCAGAGTATTTGAAAAGTCATTACAGTATGTGAAACGCTTCAGTCGCTATAAGAACCCGGATGCTGTTAGACAAGTCCGAGAGTATCCTTTAAATTGTATAGGGAAGGATGTGCAGTGCATATCTACCCTATGTTATGACTAACCACATTTTGAACTAGTATTTTACAACTACCATTTTCTTACTAGACCAAGAACTCTCTAATTAGCTGTGTATATATATTCATGGAGGGAAATACAATTTACTTTTCTCATCTAAGTTACGAACAGTAAAGTTGGCTTCATTATTTAAAATCGCATAAAAGTGGTTAAATCTTAACTACTACCATATAGAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGGTATGTGTACTGAGTCACTTAGCTTTATTAACTTACTCGTTATTATTCTACGAAAAGGTTTATCTCATCGCTCTTGAAATTTGCAGCTATGTGTTCTTGGTAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTTCCGTAGTTCCATCTTTGAAGGTATCTTTTGCCACCCTTTTTATGCCTATATTAAATTATAGTACTAGTATATTTATTGTTATTTTGATTTGCTAATTTATAAGTAGGGTACTAACTTTTAAAAGGTTCACTGTTGTATTTATCTTTCAACAATGAATATGCATCGAGTTTTAAATTACTATTAAAATTCTGAAACTAAAAGTAGAGTACAAAAAAGGAATTAAGACAAATTGCAAAAATCATTTGAAATATAATGGTAGTTGCAATTACACTCTCAAATTTTCAATGGTAAAAATGAAGTCTCATACTTATATAAGTGTTAAAATTGGACCCTCAAACTTATAATGGTGGTAGAAATTGGACCCCAAATGATAAAAATTGAATGCTCAAACTTATTCAGGTGTTATAATTTCTACAATTATGTGAGTTTGAGTCTAATTTTAATTTTTGTTTAAGTTTGGAAGCTCAATTTTACACACGAAAGTTTGAGTGTATAATTTCTCCAATCATATATCACAATACTCTAGGGGTGATTTTTACAAATTTCCATTTATTTGAGGAATATTTCAAAAGTATTTTCGAACAGCGAAAAGAAAATATCTATAGATAGAGTAGATTTACTTATAAATACTTTTGTTGGTTTCAGACTAAAGGAAGGGTACATGACGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATCAAAAAGTTTGAGTAGTTAGCTTTCTAAAACTTGGTTAAATGTGTTTTAATCCATTCTTTACATTTTCTCACCTAAAACAATTAGAAAATTTTACCCTTCTGATCACATTTTTATATATGGATAAACTTTGTAAAATGAGATCCTTGCTCAAGATGTCCAATTTCTCAGTGGGACAGATCTCTGTGGAGTCCCGCCTCGGACTCTGCAAGTTTTTGATTAAACAGGAATAGGGAGGGAGTGGAGAAATTTTTATTCCACGAACTAACGCAGATTGGGACGAAAAATGCATTCCTCATTCCTAACCTGTCTGGTTAGGCTCTATTTATTTATTTAATGTAATTATTTAATATTATGTTATTGTTATTATTATATAAATATTTCATTTAAA

mRNA sequence

TTATGGCAACCCTTCGTCCGGGTTGAAAACTAAGAAGCTGCTTCTCCTCTATCGCGCCCTTCTCTGTTTTCGCTACCTCTGATTTTGCCGCCGTCACGTCTCGCCGCCGCTGGATCTCGCCGTCGATCCTCTCTGTCTCCTGTGGTTGGACAAATCTCTAACTTCAAGCATGTCTGGAGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAATTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAGTACGAGCAACTTCAACAAAGGGCTGAGGATCCAACGAATCAAGTTTCTCAAGTATTTGAAAAGTCATTACAGTATGTGAAACGCTTCAGTCGCTATAAGAACCCGGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGCTATGTGTTCTTGGTAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTTCCGTAGTTCCATCTTTGAAGACTAAAGGAAGGGTACATGACGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATCAAAAAGTTTGAGTAGTTAGCTTTCTAAAACTTGGTTAAATGTGTTTTAATCCATTCTTTACATTTTCTCACCTAAAACAATTAGAAAATTTTACCCTTCTGATCACATTTTTATATATGGATAAACTTTGTAAAATGAGATCCTTGCTCAAGATGTCCAATTTCTCAGTGGGACAGATCTCTGTGGAGTCCCGCCTCGGACTCTGCAAGTTTTTGATTAAACAGGAATAGGGAGGGAGTGGAGAAATTTTTATTCCACGAACTAACGCAGATTGGGACGAAAAATGCATTCCTCATTCCTAACCTGTCTGGTTAGGCTCTATTTATTTATTTAATGTAATTATTTAATATTATGTTATTGTTATTATTATATAAATATTTCATTTAAA

Coding sequence (CDS)

ATGTCTGGAGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAATTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAGTACGAGCAACTTCAACAAAGGGCTGAGGATCCAACGAATCAAGTTTCTCAAGTATTTGAAAAGTCATTACAGTATGTGAAACGCTTCAGTCGCTATAAGAACCCGGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGCTATGTGTTCTTGGTAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTTCCGTAGTTCCATCTTTGAAGACTAAAGGAAGGGTACATGACGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATCAAAAAGTTTGAGTAG

Protein sequence

MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHDDEAIEKMLNDLSLIKKFE
Homology
BLAST of Bhi06G000082 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 253.8 bits (647), Expect = 7.8e-68
Identity = 126/138 (91.30%), Postives = 134/138 (97.10%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA+++VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

BLAST of Bhi06G000082 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 83.2 bits (204), Expect = 1.8e-16
Identity = 44/118 (37.29%), Postives = 75/118 (63.56%), Query Frame = 0

Query: 21  AKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREI 80
           A+C+M+CE   IL+    QL   +EDP+ ++   ++++L YV+    Y NP +VR+V E 
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  LSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHDDEAIEKMLNDLSLIKKFE 139
           L  Y +++ E+CV+ N   E+V+E ++ +PSLKTK  V  ++ ++  L +LS +KK E
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTKKEV-INQPLQDALEELSKLKKSE 205

BLAST of Bhi06G000082 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.1e-66
Identity = 126/138 (91.30%), Postives = 134/138 (97.10%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA+++VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

BLAST of Bhi06G000082 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.1e-21
Identity = 57/137 (41.61%), Postives = 94/137 (68.61%), Query Frame = 0

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

BLAST of Bhi06G000082 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 3.1e-21
Identity = 57/137 (41.61%), Postives = 94/137 (68.61%), Query Frame = 0

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

BLAST of Bhi06G000082 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 8.5e-19
Identity = 55/147 (37.41%), Postives = 87/147 (59.18%), Query Frame = 0

Query: 4   EEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAED-------------PTNQ 63
           +EEE+   LK   +   AK L+N EV+++LEH+    +    +               N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA S++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKTKGRVHDDEAIEKMLNDLSLIKKF 138
           SLK   ++ DDE ++ +L++LS ++KF
Sbjct: 132 SLK---KMEDDE-LQAILDELSNLRKF 154

BLAST of Bhi06G000082 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 86.7 bits (213), Expect = 2.3e-16
Identity = 47/126 (37.30%), Postives = 80/126 (63.49%), Query Frame = 0

Query: 6   EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  + D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHDDEAIE 125
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA +++PSL  +GR  D+E + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGRFEDEE-LR 130

Query: 126 KMLNDL 132
           ++L+D+
Sbjct: 131 QILDDI 131

BLAST of Bhi06G000082 vs. ExPASy TrEMBL
Match: A0A1S3CA78 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC103498588 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.6e-68
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Bhi06G000082 vs. ExPASy TrEMBL
Match: A0A5A7T811 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001820 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.6e-68
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Bhi06G000082 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.7e-67
Identity = 134/138 (97.10%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIS+VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Bhi06G000082 vs. ExPASy TrEMBL
Match: A0A6J1FFS7 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445330 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.7e-67
Identity = 134/138 (97.10%), Postives = 136/138 (98.55%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIS+VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

BLAST of Bhi06G000082 vs. ExPASy TrEMBL
Match: A0A5D3C019 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002310 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.9e-67
Identity = 132/138 (95.65%), Postives = 137/138 (99.28%), Query Frame = 0

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI++VPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G09920.17.8e-6891.30RNA polymerase II, Rpb4, core protein [more]
AT4G15950.11.8e-1637.29RNA polymerase II, Rpb4, core protein [more]
Match NameE-valueIdentityDescription
O488901.1e-6691.30DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB... [more]
O155143.1e-2141.61DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE... [more]
Q9D7M83.1e-2141.61DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P... [more]
Q54S048.5e-1937.41DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689... [more]
Q9VEA52.3e-1637.30DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G... [more]
Match NameE-valueIdentityDescription
A0A1S3CA781.6e-6897.83DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7T8111.6e-6897.83DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1JVN31.7e-6797.10DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FFS71.7e-6797.10DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A5D3C0193.9e-6795.65DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006590RNA polymerase Rpb4/RPC9, coreSMARTSM00657rpol4neu2coord: 16..138
e-value: 6.6E-56
score: 201.7
IPR038324Rpb4/RPC9 superfamilyGENE3D1.20.1250.40coord: 2..138
e-value: 7.9E-51
score: 173.3
IPR005574RNA polymerase subunit RPB4/RPC9PFAMPF03874RNA_pol_Rpb4coord: 23..132
e-value: 2.8E-23
score: 82.6
NoneNo IPR availablePANTHERPTHR21297:SF3DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKEcoord: 1..138
NoneNo IPR availablePANTHERPTHR21297DNA-DIRECTED RNA POLYMERASE IIcoord: 1..138
IPR010997HRDC-like superfamilySUPERFAMILY47819HRDC-likecoord: 3..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M000082Bhi06M000082mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031990 mRNA export from nucleus in response to heat stress
biological_process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process GO:0045948 positive regulation of translational initiation
biological_process GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0044237 cellular metabolic process
biological_process GO:0006352 DNA-templated transcription, initiation
cellular_component GO:0000932 P-body
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0030880 RNA polymerase complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0031369 translation initiation factor binding