Bhi06G000064 (gene) Wax gourd (B227) v1

Overview


Name	Bhi06G000064
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Cytochrome b561 domain-containing protein
Location	chr6: 1582939 .. 1585721 (+)
RNA-Seq Expression	Bhi06G000064
Synteny	Bhi06G000064

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGAAAAAGAAAAAGGCATCTCTCTTACGTCTTAATTTCCAAATATTCTGTAATGAAAAATTAAGCTTAAATTTTCCATTTCAACTCTCCATGGCGATGACGGAGATCCACGTCTGAAACTCCATTGGAAGATCAGTATTTCTCTTTAACTCGGCTGAAATTCCTCTCAAATTTCGCTACGATTTCCACCATTGAAGATCCTAAAACCCCAATTCTTTCCTCCATCGTCTCTTCCGCTGAAGAATCAATGGCGGTTCCCGTGGTTCAGTTCCCGATCTTCATGGCTGTCAGAGTTCTCGGAATGCTCGTGGCAGCTCTTCTTTTCATTTGGACCTTGCATTTCCGTGGAGGATTGGCACTTATCTCAGATAACAAGGATCATATCTTCAATGTAATTACCTACTTCCTTTATTTTCGCAAATGATTCTGCTATTGTCTGTTTTTTTTTCTTTTATGAATTGATACGCATTTTTGATGGGGCTTGAGTTTATAGATGCTCACGAGTCTTCACTGGATCTACTAATTTAGTTTTTCGTCGTTCTGCTTTGGATTTTGAATTTGCGTGTTTGAAATAAATTTTGTATTGTTTTAGTTTCTATATGATGGCAATCTCATTGAAGTTTGTTTGTTTGTCTCGGAATTGGGCTCAACTTAAATAACATACTTGATGATCTTGTATATGGAGATAGAAATATTGCCTTTTAATTCTCCATTTTCCTTTTTGTTGTCCTGTTTTTTTGGTGAAGTTGCTCTTGCTCCTGTTTTGTAGGTCCATCCAGTTCTTATGGTGATTGGACTTATTCTTCTAAATGGTGAAGGTAATTATACGTTCTCTTTGTATTTAATTCGTAGTTACTATACTATTGAGGGGTTTGTATATATATGTGTGTGTAATTTATTCAATTAACACATAACATCTGTTAGCGATGGATTGATTTGTGGTTTCAATCCCCCATCCCTATTATACTTAAAAGAAACTATATATTGATGATAATGGAAACAAAAGAGGGAGAATGCAGCTGGATTTAATATTTGTGGTTTACAATCTTTAAGAAAACGAGGATAGTGGGCAAGGTTTTCTTTAACAGTATGAAACATTTCCTGGCGTTCATCATGTAGGTTTTAGGTTTTGTCCAGCAAATGTGTTATTATACAAAACCAGCTAAAGCTATGGTTTCGAATCATTTATGAAATATGTATTCTGAAATCATTTGCAAACTTAGTTTTAAGTTATTCTCCCACTATGCTGGTGCTTTCAATGATTTTGTTCACCATTCTTAGATATATTGATGCAGCAATGTTAGCATACAAGACAGTTTCAGGAACCAAAAACTTCAAAAAACTAGTTCATCTTGCACTCCAATTTGTGGCATTCTTTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTCCACAATGACAAAGGAATCGATAACTTTTACAGTCTTCATTCATGGTTGGGCTTAGTTTCTATCTTTCTGTTTGGCATCCAGGTTCGTAAATCGCAATATCTGCTCATAAATCGTAATACTTGTCTTAATCGGAATGTTTTTATTTCTTCAGTTTAATCATGTAGTCATGACATTTTGTAGCTCATGAATAGTTGTATTTTGTATTTGGATGCGATGAGGGTACTATGAGTGTATTGGTCTACTTTAGATATCCGAGTGCAACTGCTGATTCTTAGCTTCTTAGTATTTGAGTTTCTATGCTTATTATGTCTCATTGTATTTTGAGTAGTAGTCTCTTTTCATTCTATTTTTTTTTTAAATCATAATATTTTATTGTATGATTTACTTTCTAGCCTGCCTGTTGAGCTACATGATTCTAATTCATTAGGACGACTCCATTCGAACAGTGGTCTGCAGGTTTTGTAACCTTTTGGTACCCAGGTGGGTCTAGAAACAGCAGAGCTACTCTGTTGCCATGGCATGTTTTCTTTGGCGTTTACATATATGGCCTTTCTGTCGCTACAACTGTAACTGGATTGTTGGAAAAAGCTACATTTCTTCAAACCAGCAAGGTAATATTACGCTATTCGAACGAGGCTTTGCTGATAAATTCATTGGGTGTCCTCATTGTTCTCTTGGGTGGTCTCGTAATTCTGGCGGTTATCGCTCCGTCGAATGCCAAAGGTGATATTAACAGAGGGCTAGCAGAATAGGATGAAAACATGATGCTGCTTTGAAATGGAACATCAGCGAGACAGATCTTGTTGTAACTTGTAAGCATGTCAATTGCTCAAAATCAAACTTGCCTTTATTTTTACTCGAGACTGTTGATTCATCCTTAACATAGAAATCACATTATAGGTTGAGAAGCAAACGGATTCTTTAGTTGAAAATAAGATGGTCATGTTTGTGGATTGGAACCGATTTTATGACTTCAGTGCCATAAGAAGTAAACAGTTTTAATTGAAAATTTTCGATTTGGTTCAGTTCGTCTACCAAACAGAATCAAGCCGTGGGCAGAGTCAAATAAGAATGCATTGTACAAGCTGATGAAATAAAATACGGAGCTCCCACACCGTATTTTTTTGGTCAACTTTTATTTCTTGATTTCTCTCACCCTTTTTATAGGAACTTTGACTGTCATTGTATCGTCAACTAATTAAACGTAGGAAAAGTCTATCCATCTCCGATGCTTTATTCGGTGGCAAATAACTGGCAAAAGGTAATCATCTCTCAATACACTAAAAAGCA

mRNA sequence

AAAAGAAAAAGAAAAAGGCATCTCTCTTACGTCTTAATTTCCAAATATTCTGTAATGAAAAATTAAGCTTAAATTTTCCATTTCAACTCTCCATGGCGATGACGGAGATCCACGTCTGAAACTCCATTGGAAGATCAGTATTTCTCTTTAACTCGGCTGAAATTCCTCTCAAATTTCGCTACGATTTCCACCATTGAAGATCCTAAAACCCCAATTCTTTCCTCCATCGTCTCTTCCGCTGAAGAATCAATGGCGGTTCCCGTGGTTCAGTTCCCGATCTTCATGGCTGTCAGAGTTCTCGGAATGCTCGTGGCAGCTCTTCTTTTCATTTGGACCTTGCATTTCCGTGGAGGATTGGCACTTATCTCAGATAACAAGGATCATATCTTCAATGTCCATCCAGTTCTTATGGTGATTGGACTTATTCTTCTAAATGGTGAAGCAATGTTAGCATACAAGACAGTTTCAGGAACCAAAAACTTCAAAAAACTAGTTCATCTTGCACTCCAATTTGTGGCATTCTTTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTCCACAATGACAAAGGAATCGATAACTTTTACAGTCTTCATTCATGGTTGGGCTTAGTTTCTATCTTTCTGTTTGGCATCCAGTGGTCTGCAGGTTTTGTAACCTTTTGGTACCCAGGTGGGTCTAGAAACAGCAGAGCTACTCTGTTGCCATGGCATGTTTTCTTTGGCGTTTACATATATGGCCTTTCTGTCGCTACAACTGTAACTGGATTGTTGGAAAAAGCTACATTTCTTCAAACCAGCAAGGTAATATTACGCTATTCGAACGAGGCTTTGCTGATAAATTCATTGGGTGTCCTCATTGTTCTCTTGGGTGGTCTCGTAATTCTGGCGGTTATCGCTCCGTCGAATGCCAAAGGTGATATTAACAGAGGGCTAGCAGAATAGGATGAAAACATGATGCTGCTTTGAAATGGAACATCAGCGAGACAGATCTTGTTGTAACTTGTTGAGAAGCAAACGGATTCTTTAGTTGAAAATAAGATGGTCATGTTTGTGGATTGGAACCGATTTTATGACTTCAGTGCCATAAGAAGTAAACAGTTTTAATTGAAAATTTTCGATTTGGTTCAGTTCGTCTACCAAACAGAATCAAGCCGTGGGCAGAGTCAAATAAGAATGCATTGTACAAGCTGATGAAATAAAATACGGAGCTCCCACACCGTATTTTTTTGGTCAACTTTTATTTCTTGATTTCTCTCACCCTTTTTATAGGAACTTTGACTGTCATTGTATCGTCAACTAATTAAACGTAGGAAAAGTCTATCCATCTCCGATGCTTTATTCGGTGGCAAATAACTGGCAAAAGGTAATCATCTCTCAATACACTAAAAAGCA

Coding sequence (CDS)

ATGGCGGTTCCCGTGGTTCAGTTCCCGATCTTCATGGCTGTCAGAGTTCTCGGAATGCTCGTGGCAGCTCTTCTTTTCATTTGGACCTTGCATTTCCGTGGAGGATTGGCACTTATCTCAGATAACAAGGATCATATCTTCAATGTCCATCCAGTTCTTATGGTGATTGGACTTATTCTTCTAAATGGTGAAGCAATGTTAGCATACAAGACAGTTTCAGGAACCAAAAACTTCAAAAAACTAGTTCATCTTGCACTCCAATTTGTGGCATTCTTTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTCCACAATGACAAAGGAATCGATAACTTTTACAGTCTTCATTCATGGTTGGGCTTAGTTTCTATCTTTCTGTTTGGCATCCAGTGGTCTGCAGGTTTTGTAACCTTTTGGTACCCAGGTGGGTCTAGAAACAGCAGAGCTACTCTGTTGCCATGGCATGTTTTCTTTGGCGTTTACATATATGGCCTTTCTGTCGCTACAACTGTAACTGGATTGTTGGAAAAAGCTACATTTCTTCAAACCAGCAAGGTAATATTACGCTATTCGAACGAGGCTTTGCTGATAAATTCATTGGGTGTCCTCATTGTTCTCTTGGGTGGTCTCGTAATTCTGGCGGTTATCGCTCCGTCGAATGCCAAAGGTGATATTAACAGAGGGCTAGCAGAATAG

Protein sequence

MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLILLNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWLGLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKATFLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE

Homology

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BLAST of Bhi06G000064 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 332.0 bits (850), Expect = 3.8e-91
Identity = 161/227 (70.93%), Postives = 191/227 (84.14%), Query Frame = 0


Query: 1   MAVPVV-QFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLI 60
           MAVPV+  FPIFM VRVLG ++AAL+  WT+H+RGGLAL SDNKDHIFNVHPV+MVIGLI
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 61  LLNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSW 120
           L NGEAMLAYK+V GTKN KKLVHL LQ  AF LSLIG+WAALKFH DKGI+NFYSLHSW
Sbjct: 61  LFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSW 120

Query: 121 LGLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKA 180
           LGL  +FLF  QW+AGFVT+WYPGGSRNSRA+L+PWHVF G+ IY L++ T  TG+LEK 
Sbjct: 121 LGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKV 180

Query: 181 TFLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDI 227
           TFLQ ++VI RYS EA+L+N++GVLI++LGG VIL V+ P + K  +
Sbjct: 181 TFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTPVSGKDQV 227

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BLAST of Bhi06G000064 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 186.8 bits (473), Expect = 2.0e-47
Identity = 95/220 (43.18%), Postives = 139/220 (63.18%), Query Frame = 0


Query: 1   MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLIL 60
           MAV +    +      L ++ A ++ +W++ +RGGLA  + NK+ IFN+HPVLM+IG I+
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFII 60

Query: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120
           L GEA+++YK++   K  KKL+HL L  +A  L + GI AA K HN+  I N YSLHSW+
Sbjct: 61  LGGEAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWI 120

Query: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKAT 180
           G+  I L+G QW   F+ F++PGGS N ++ LLPWH   G+++Y L+V     G LEK T
Sbjct: 121 GIGVISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLT 180

Query: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPS 221
           FL+    + +Y +EA LIN   ++ +L G  V+L   A S
Sbjct: 181 FLENGG-LDKYGSEAFLINFTAIITILFGAFVVLTASAES 219

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BLAST of Bhi06G000064 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 177.2 bits (448), Expect = 1.6e-44
Identity = 98/211 (46.45%), Postives = 138/211 (65.40%), Query Frame = 0


Query: 10  IFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLILLNGEAMLAY 69
           + +  R+ G++VA  +  W L F   L L          +HP+LMVIG IL++GEA+L +
Sbjct: 11  LVLFARLSGLVVAVSVLYWAL-FLPNLGL------SYSTLHPLLMVIGFILVSGEAILIH 70

Query: 70  KTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGI-DNFYSLHSWLGLVSIFLF 129
           + + G++  KK VHL LQ +A   ++ GIW   KFH  +G+  NFYSLHSW+GL+S+ LF
Sbjct: 71  RWLPGSRKTKKAVHLWLQGMALASAVFGIWT--KFHYQRGVFANFYSLHSWMGLLSVSLF 130

Query: 130 GIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKATFLQTSKVI 189
             QW  GF++FW+ G  R +R T LPWHVF G+Y YGL++AT  TGLLEK TFLQT + +
Sbjct: 131 AAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNV 190

Query: 190 LRYSNEALLINSLGVLIVLLGGLVILAVIAP 220
            R  +E++ +N LG+ + LLG +VI A I P
Sbjct: 191 PRRGSESMTVNGLGLGLALLGCIVITAAILP 212

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BLAST of Bhi06G000064 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 144.8 bits (364), Expect = 8.5e-35
Identity = 77/205 (37.56%), Postives = 114/205 (55.61%), Query Frame = 0


Query: 12  MAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLILLNGEAMLAYKT 71
           +     G+L   L+ IW LH+R G+   SDN   + NVHP LM  G + L G+AM+ YKT
Sbjct: 19  LVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQAMMTYKT 78

Query: 72  VSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWLGLVSIFLFGIQ 131
              +   +K+VH  L  +   L ++GI AA +FH+   + +  SLHSW+GL +  L G+Q
Sbjct: 79  AYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGVQ 138

Query: 132 WSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKATFLQTSKVILRY 191
           W  G  TF  P  S  +R  ++PWHV  G  +  + +   +TGL+++AT L  S      
Sbjct: 139 WLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQST----- 198

Query: 192 SNEALLINSLGVLIVLLGGLVILAV 217
           + E+ LIN LG+ I+L G  V  +V
Sbjct: 199 NAESRLINFLGLAILLFGVSVDFSV 218

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BLAST of Bhi06G000064 vs. ExPASy Swiss-Prot
Match: Q9SWS1 (Transmembrane ascorbate ferrireductase 2 OS=Arabidopsis thaliana OX=3702 GN=CYB561B PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 5.3e-90
Identity = 161/227 (70.93%), Postives = 191/227 (84.14%), Query Frame = 0


Query: 1   MAVPVV-QFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLI 60
           MAVPV+  FPIFM VRVLG ++AAL+  WT+H+RGGLAL SDNKDHIFNVHPV+MVIGLI
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 61  LLNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSW 120
           L NGEAMLAYK+V GTKN KKLVHL LQ  AF LSLIG+WAALKFH DKGI+NFYSLHSW
Sbjct: 61  LFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSW 120

Query: 121 LGLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKA 180
           LGL  +FLF  QW+AGFVT+WYPGGSRNSRA+L+PWHVF G+ IY L++ T  TG+LEK 
Sbjct: 121 LGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKV 180

Query: 181 TFLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDI 227
           TFLQ ++VI RYS EA+L+N++GVLI++LGG VIL V+ P + K  +
Sbjct: 181 TFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTPVSGKDQV 227

[Close]

BLAST of Bhi06G000064 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-50
Identity = 102/216 (47.22%), Postives = 143/216 (66.20%), Query Frame = 0


Query: 9   PIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLILLNGEAMLA 68
           P       L +  A ++ +W + FRGGLA  +DNK+ IFNVHPVLM+IG I+L  EA++ 
Sbjct: 11  PFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSEAIMI 70

Query: 69  YKTVSG-TKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWLGLVSIFL 128
           YK       +  KL+HL L  +A  L  +GI+ A KFHN+ GI N YSLHSWLG+ +I L
Sbjct: 71  YKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIGTISL 130

Query: 129 FGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKATFLQTSKV 188
           +GIQW  GFV F+YPG + + R   LPWHV FG+++Y L++AT   GLLEK TFLQ+S  
Sbjct: 131 YGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQSSG- 190

Query: 189 ILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAK 224
           + +Y  EA L+N  G+++ L G  V++A +AP++ +
Sbjct: 191 LDKYGAEAFLVNFTGLVVALFGAAVVVAAVAPAHVE 225

[Close]

BLAST of Bhi06G000064 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-49
Identity = 98/212 (46.23%), Postives = 145/212 (68.40%), Query Frame = 0


Query: 9   PIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLILLNGEAMLA 68
           P   A   L +  AA++ +W++ FRGGLA+ S NK+ IFNVHPVLM+IG +++ GEA++ 
Sbjct: 11  PFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGEAIMV 70

Query: 69  YKTV-SGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWLGLVSIFL 128
           Y+ + +   +  KL+HL L  +A  L  +GI+ A K HN+ GI N YSLHSW+G+ +I L
Sbjct: 71  YRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITL 130

Query: 129 FGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKATFLQTSKV 188
           +GIQW  GFVTF++PG + N +  +LPWHV FG+++Y L++A    G LEK TFL++S  
Sbjct: 131 YGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLESSG- 190

Query: 189 ILRYSNEALLINSLGVLIVLLGGLVILAVIAP 220
           + +Y  EA L+N   +++VL G  V++A IAP
Sbjct: 191 LDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

[Close]

BLAST of Bhi06G000064 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-49
Identity = 97/212 (45.75%), Postives = 144/212 (67.92%), Query Frame = 0


Query: 9   PIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLILLNGEAMLA 68
           P   A   L +  AA++ +W ++FRGGLA+ + NK+ IFNVHPVLM+IG I++ GEA++ 
Sbjct: 11  PFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGEAIMV 70

Query: 69  YKTV-SGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWLGLVSIFL 128
           Y+ + +      KL+HL L  +A  L  +GI+ A K HN+ GI N YSLHSW+G+ +I L
Sbjct: 71  YRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITL 130

Query: 129 FGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKATFLQTSKV 188
           +GIQW  GFVTF++PG + N +  +LPWH+ FG+++Y L++A    G LEK TFL++S  
Sbjct: 131 YGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLESSG- 190

Query: 189 ILRYSNEALLINSLGVLIVLLGGLVILAVIAP 220
           + +Y  EA L+N   +++VL G  V++A IAP
Sbjct: 191 LDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

[Close]

BLAST of Bhi06G000064 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.3e-48
Identity = 100/224 (44.64%), Postives = 145/224 (64.73%), Query Frame = 0


Query: 1   MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLIL 60
           M + V   P       L +  A ++ +W +HFRGGLA  + NK+ IFNVHPVLM+IG I+
Sbjct: 1   MGLGVRAAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYII 60

Query: 61  LNGEAMLAYKTVSGTK-NFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSW 120
           L  EA++ YK +   K +  KL+HL L  +A     +GI+ A KFHN+ GI N YSLHSW
Sbjct: 61  LGSEAIMVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSW 120

Query: 121 LGLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKA 180
           LG+ +I L+GIQW  GFV F++P  S + R  +LPWH+ FG+++Y L++AT   G LEK 
Sbjct: 121 LGIGTICLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKL 180

Query: 181 TFLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAK 224
           TFLQ+S  + +Y  EA L+N   +++VL G  V++A ++P+  +
Sbjct: 181 TFLQSSG-LDKYGAEAFLVNFTALIVVLFGASVVVAAVSPARVE 223

[Close]

BLAST of Bhi06G000064 vs. ExPASy TrEMBL
Match: Q3LGX4 (Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561B PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.7e-121
Identity = 227/232 (97.84%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLIL 60
           MAVP+VQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALIS+NKDHIFNVHPVLMVIGLIL
Sbjct: 1   MAVPLVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISENKDHIFNVHPVLMVIGLIL 60

Query: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKAT 180
           GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFG+YIYGLS+ATTVTGLLEKAT
Sbjct: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGIYIYGLSIATTVTGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE 233
           FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGD+NRGLAE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDVNRGLAE 232

[Close]

BLAST of Bhi06G000064 vs. ExPASy TrEMBL
Match: A0A5A7TD04 (Putative transmembrane ascorbate ferrireductase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001910 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 7.9e-121
Identity = 227/232 (97.84%), Postives = 230/232 (99.14%), Query Frame = 0


Query: 1   MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLIL 60
           MAVPVVQFPIF+AVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMV GLIL
Sbjct: 1   MAVPVVQFPIFLAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVTGLIL 60

Query: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKTVSGTKNFKKLVHL LQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLTLQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKAT 180
           GLVS+FLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLS+ATTVTGLLEKAT
Sbjct: 121 GLVSVFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSIATTVTGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE 233
           FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE 232

[Close]

BLAST of Bhi06G000064 vs. ExPASy TrEMBL
Match: A0A1S3CA72 (probable transmembrane ascorbate ferrireductase 2 OS=Cucumis melo OX=3656 GN=LOC103498586 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.3e-120
Identity = 227/232 (97.84%), Postives = 230/232 (99.14%), Query Frame = 0


Query: 1   MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLIL 60
           MAVPVVQFPIF+AVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMV GLIL
Sbjct: 1   MAVPVVQFPIFLAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVTGLIL 60

Query: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKTVSGTKNFKKLVHL LQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLTLQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKAT 180
           GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLS+ATTVTGLLEKAT
Sbjct: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSIATTVTGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE 233
           FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDI+RGLAE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDIDRGLAE 232

[Close]

BLAST of Bhi06G000064 vs. ExPASy TrEMBL
Match: A0A0A0KVW9 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G643950 PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.4e-117
Identity = 222/232 (95.69%), Postives = 227/232 (97.84%), Query Frame = 0


Query: 1   MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLIL 60
           MAVPVVQFPIF+AVRVLGMLVAALLF+WTLHFRGGLALISDNKDHIFNVHPVLMV GLIL
Sbjct: 1   MAVPVVQFPIFLAVRVLGMLVAALLFMWTLHFRGGLALISDNKDHIFNVHPVLMVTGLIL 60

Query: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKTVSGTKNFKKLVHL LQFVAFFLSLIGIWAALKFHNDKGI+NFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLTLQFVAFFLSLIGIWAALKFHNDKGIENFYSLHSWL 120

Query: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKAT 180
           GLVSIFLFG+QWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLS+ATTVTGLLEKAT
Sbjct: 121 GLVSIFLFGVQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSIATTVTGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE 233
           FLQTSKVI RYSNEALLINSLGVLIVLLGGLVILAVIAPS AKGDINRG AE
Sbjct: 181 FLQTSKVISRYSNEALLINSLGVLIVLLGGLVILAVIAPSYAKGDINRGPAE 232

[Close]

BLAST of Bhi06G000064 vs. ExPASy TrEMBL
Match: A0A6J1K1L7 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489314 PE=4 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.0e-112
Identity = 212/232 (91.38%), Postives = 222/232 (95.69%), Query Frame = 0


Query: 1   MAVPVVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVIGLIL 60
           MAVP VQFPIFMAVRVLGM+VAAL+FIWTLHFRGGLAL SDNKD IFNVHPVLMVIGLIL
Sbjct: 1   MAVPAVQFPIFMAVRVLGMVVAALVFIWTLHFRGGLALSSDNKDLIFNVHPVLMVIGLIL 60

Query: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKTVSGTKNFKKLVHLALQFVA FLSLIGIWAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVALFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSVATTVTGLLEKAT 180
           GLVS+FLFGIQW AGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGL +ATTVTGLLEKAT
Sbjct: 121 GLVSVFLFGIQWGAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLCIATTVTGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE 233
           FLQTSKVILRYSNEALLINSLG+++V+LGGL+ILAVIA SN KGD+ RG AE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGIVVVVLGGLIILAVIARSNNKGDVYRGPAE 232

The following BLAST results are available for this feature:

BLAST of Bhi06G000064 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G38630.1	3.8e-91	70.93	cytochrome B561-1	[more]
AT4G25570.1	2.0e-47	43.18	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT1G26100.1	1.6e-44	46.45	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT1G14730.1	8.5e-35	37.56	Cytochrome b561/ferric reductase transmembrane protein family	[more]

BLAST of Bhi06G000064 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SWS1	5.3e-90	70.93	Transmembrane ascorbate ferrireductase 2 OS=Arabidopsis thaliana OX=3702 GN=CYB5...	[more]
Q7XMK3	1.4e-50	47.22	Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa...	[more]
Q6I681	1.6e-49	46.23	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z...	[more]
C4IYS8	1.6e-49	45.75	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2...	[more]
A3A9H6	1.3e-48	44.64	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]

BLAST of Bhi06G000064 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q3LGX4	2.7e-121	97.84	Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561B PE=2 SV=1	[more]
A0A5A7TD04	7.9e-121	97.84	Putative transmembrane ascorbate ferrireductase 2 OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3CA72	2.3e-120	97.84	probable transmembrane ascorbate ferrireductase 2 OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A0A0KVW9	1.4e-117	95.69	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G64...	[more]
A0A6J1K1L7	1.0e-112	91.38	probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 49..175 e-value: 1.3E-53 score: 194.1
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 49..181 e-value: 1.3E-46 score: 158.2
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 13..217 score: 46.681896
None	No IPR available	GENE3D	1.20.120.1770		coord: 1..229 e-value: 1.1E-88 score: 298.2
None	No IPR available	PANTHER	PTHR10106:SF49	B561, PUTATIVE-RELATED	coord: 1..229
None	No IPR available	CDD	cd08766	Cyt_b561_ACYB-1_like	coord: 40..183 e-value: 9.70349E-74 score: 218.715
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 1..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000064	Bhi06M000064	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity