Bhi05G001477 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G001477
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Beta-galactosidase
Location	chr5: 55621239 .. 55623278 (+)
RNA-Seq Expression	Bhi05G001477
Synteny	Bhi05G001477

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACCAATGGGACTATTTTACGTTTGCAATACCATAGTGGACCAAACTTCGGTAGAATAAGTGTTTTCTTTACAACAACGAGGTACTATGATGAAGCACCACTCGATGAATATGGTAAGTGGGTTGCATGTCTAAGTACGATCAATCATTGACCTTGAAGAACGTGCTTGAAAGCGCACTCAATTGTCTAAAATTTTGCACTGTTATGATGTGAAAGGCTTACAAAGGGAACCGAAATGGGGTCATTTCAAGGACGTGCACAAGGCATTGAATCTGTGCAAGAAACCTCTCCTTTGGGGAACTCCTGAAACCCAAGTGATGGGCAAGGGTTTAGAGGTACAATGATCATTTTTATACATTCGATGTAATGTTTTATGTGAAGGCACTTGGCTGATCCTTCAAAACCACCAAGTTATGGCCAATTGATCAACTTCTTGTACCATGTATAGGCTCGTTTTTATGAGAAGCCAGGAACTAATATTTGTGCAACTTTCTTGGCCAATAATGACACAAAGAGTGCACAAACTATTACCTTCAGAGGACGGAAATATCTTCTTCCACCTCGATCAATTAGCATACTTTCTGACTCTAACACTGTTATCTAAAATACTAAGACGGTAAAACTCCTGTCTCATGAAAACTTAGTGATGGAAAGTTTAATAAAATAGGTTATGTAGGCTGTTAAAGTATAAATCTTACCGCAATTCAGGTTCATGGTTTCTCGAGACTCTAATTGACTCTTCCATAACCATTTGCCAAAAAAAAAAAATACAAATTGTATCACAACACAATGATTTTGGACAAATAATCACTACTTTCTTTTATCAATAATACAGGATAGTGAAGCATACATGGAACACAGATTTGCAGGGCCTCGATATATAACAATCCTTGGTTTAAATACCGGAACACTTGATATTTCAAAAAATTGTTAGGGACACCAGGTAATATTACAAGCAATCCCTTCACACCCAACTAATCAAGTAAATGAAGTTGATTGGCAAAACATACAAGTCATAGGTTGTCCGACTTAAATACTCACACTCTTGCAGGTTGGTCTGAATGGAGAAAAAGTTAAAGCTTTCACTCAAGGAGGATCACATCGAGTCGACTGGAGCAAAATCAATGAAGAAAAAACTGCTCTCACATGGTTCAAGGTAATGATAATAAGCAGTTTTGTCGACTCTTTTCCCAGTTTTGGCATTGGAGATAAAATGTACCAACTCCATGAAGATACTATGATTCAACTAAACTCACTCATTAATCTTGCTGACATATTTTGATGCTCCAGAAGCAAATGGTCTTGTGGTTATTAGAATGAATGGAATGGGGAAGGGATAGATTTGGGTGAATGGTAAAAGCATCGGTCAGTATTGGATGTCCTACTTATCCCCACTAAAAATGGCAACTCAATCAGAGTAAACAAACACCCTTCTCCTTTTCCCTACTCATTAACATCTCTTTAAACTCAATACTAAAAAAAGTTTTGTCCAAGAAAACAAATGTTGCTCTTTCTTAGGTACCACATTCCTAGAACCTTCATAAAGCCGTCAGAAAATCTACTGGTAATATTGGAAGAAGAGGATCACACATCTGAAAAGGTTGAAATAGTACTCGTGAACAGAAACACAATTTACAGCTTCATTACCAAATATCATCCACCAAATGTCAAGTCTTGGGAAAGGAAACACAGGCAATTCAGAGCTGTAGTAGATGATTTGACAACAGGTGCCCACCTCCAGTGTCCCGACGAAAAAAAGATCGCCTCCATTGAATTTGTTAGCTACGGTGATCCTTCTGATGTTTATGGAAACTATCAACATGGGAAATGCCACTCATCGTCCGAGACACAAAACTTGTCGAACAGGTAAAAGAAACTAATACAAATACAAACCCCTTGTTTCTGTTTGATTATCCTTTTCAAATGGTTTTTTTTTTTTTTTTTTTTTTGCAGCATTGTATGTGTAATAAGAACTTTTCAGTGCCAATGGATACATTCAACAACTTCAAGAATGAATGTGATGAGAAGACACTAG

mRNA sequence

ATGGCAACCAATGGGACTATTTTACGTTTGCAATACCATAGTGGACCAAACTTCGGTAGAATAAGTGTTTTCTTTACAACAACGAGGTACTATGATGAAGCACCACTCGATGAATATGGCTTACAAAGGGAACCGAAATGGGGTCATTTCAAGGACGTGCACAAGGCATTGAATCTGTGCAAGAAACCTCTCCTTTGGGGAACTCCTGAAACCCAAGTGATGGGCAAGGGTTTAGAGGTTGGTCTGAATGGAGAAAAAGTTAAAGCTTTCACTCAAGGAGGATCACATCGAGTCGACTGGAGCAAAATCAATGAAGAAAAAACTGCTCTCACATGGTTCAAGAATGAATGGAATGGGGAAGGGATAGATTTGGGTGAATGGTACCACATTCCTAGAACCTTCATAAAGCCGTCAGAAAATCTACTGGTAATATTGGAAGAAGAGGATCACACATCTGAAAAGGTTGAAATAGTACTCGTGAACAGAAACACAATTTACAGCTTCATTACCAAATATCATCCACCAAATGTCAAGTCTTGGGAAAGGAAACACAGGCAATTCAGAGCTGTAGTAGATGATTTGACAACAGGTGCCCACCTCCAGTGTCCCGACGAAAAAAAGATCGCCTCCATTGAATTTGTTAGCTACGGTGATCCTTCTGATGTTTATGGAAACTATCAACATGGGAAATGCCACTCATCGTCCGAGACACAAAACTTGTCGAACAGCATTGTATGTGTAATAAGAACTTTTCAGTGCCAATGGATACATTCAACAACTTCAAGAATGAATGTGATGAGAAGACACTAG

Coding sequence (CDS)

ATGGCAACCAATGGGACTATTTTACGTTTGCAATACCATAGTGGACCAAACTTCGGTAGAATAAGTGTTTTCTTTACAACAACGAGGTACTATGATGAAGCACCACTCGATGAATATGGCTTACAAAGGGAACCGAAATGGGGTCATTTCAAGGACGTGCACAAGGCATTGAATCTGTGCAAGAAACCTCTCCTTTGGGGAACTCCTGAAACCCAAGTGATGGGCAAGGGTTTAGAGGTTGGTCTGAATGGAGAAAAAGTTAAAGCTTTCACTCAAGGAGGATCACATCGAGTCGACTGGAGCAAAATCAATGAAGAAAAAACTGCTCTCACATGGTTCAAGAATGAATGGAATGGGGAAGGGATAGATTTGGGTGAATGGTACCACATTCCTAGAACCTTCATAAAGCCGTCAGAAAATCTACTGGTAATATTGGAAGAAGAGGATCACACATCTGAAAAGGTTGAAATAGTACTCGTGAACAGAAACACAATTTACAGCTTCATTACCAAATATCATCCACCAAATGTCAAGTCTTGGGAAAGGAAACACAGGCAATTCAGAGCTGTAGTAGATGATTTGACAACAGGTGCCCACCTCCAGTGTCCCGACGAAAAAAAGATCGCCTCCATTGAATTTGTTAGCTACGGTGATCCTTCTGATGTTTATGGAAACTATCAACATGGGAAATGCCACTCATCGTCCGAGACACAAAACTTGTCGAACAGCATTGTATGTGTAATAAGAACTTTTCAGTGCCAATGGATACATTCAACAACTTCAAGAATGAATGTGATGAGAAGACACTAG

Protein sequence

MATNGTILRLQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKKPLLWGTPETQVMGKGLEVGLNGEKVKAFTQGGSHRVDWSKINEEKTALTWFKNEWNGEGIDLGEWYHIPRTFIKPSENLLVILEEEDHTSEKVEIVLVNRNTIYSFITKYHPPNVKSWERKHRQFRAVVDDLTTGAHLQCPDEKKIASIEFVSYGDPSDVYGNYQHGKCHSSSETQNLSNSIVCVIRTFQCQWIHSTTSRMNVMRRH

Homology

[Close]

BLAST of Bhi05G001477 vs. TAIR 10
Match: AT4G35010.1 (beta-galactosidase 11 )

HSP 1 Score: 139.0 bits (349), Expect = 5.4e-33
Identity = 115/498 (23.09%), Postives = 165/498 (33.13%), Query Frame = 0


Query: 4   NGT-ILRLQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NGT +    YH G NFGR S  + TTRYYD+APLDEYGL++EPK+GH K +H ALNLCKK
Sbjct: 300 NGTHVNYYMYHGGTNFGRTSAHYVTTRYYDDAPLDEYGLEKEPKYGHLKHLHNALNLCKK 359

Query: 64  PLLWGTPETQVMGK---------------------------------------------- 123
           PLLWG P+T+  GK                                              
Sbjct: 360 PLLWGQPKTEKPGKDTEIRYYEQPGTKTCAAFLANNNTEAAETIKFKGREYVIAPRSISI 419

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 420 LPDCKTVVYNTAQIVSQHTSRNFMKSKKANKKFDFKVFTETLPSKLEGNSYIPVELYGLT 479

Query: 184 ------------------------------------------------------------ 239
                                                                       
Sbjct: 480 KDKTDYGWYTTSFKVHKNHLPTKKGVKTFVRIASLGHALHAWLNGEYLGSGHGSHEEKSF 539

[Close]

BLAST of Bhi05G001477 vs. TAIR 10
Match: AT2G16730.1 (glycosyl hydrolase family 35 protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.5e-30
Identity = 113/493 (22.92%), Postives = 166/493 (33.67%), Query Frame = 0


Query: 4   NGT-ILRLQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NGT +    YH G NFGR S  + TTRYYD+APLDE+GL+REPK+GH K +H ALNLCKK
Sbjct: 303 NGTHVNYYMYHGGTNFGRTSAHYVTTRYYDDAPLDEFGLEREPKYGHLKHLHNALNLCKK 362

Query: 64  PLLWGTP-------ETQVM----------------------------------------- 123
            LLWG P       ET++                                          
Sbjct: 363 ALLWGQPRVEKPSNETEIRYYEQPGTKVCAAFLANNNTEAAEKIKFRGKEYLIPHRSISI 422

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 423 LPDCKTVVYNTGEIISHHTSRNFMKSKKANKNFDFKVFTESVPSKIKGDSFIPVELYGLT 482

Query: 184 -------------------------GK--------------------------------- 234
                                    GK                                 
Sbjct: 483 KDESDYGWYTTSFKIDDNDLSKKKGGKPNLRIASLGHALHVWLNGEYLGNGHGSHEEKSF 542

[Close]

BLAST of Bhi05G001477 vs. TAIR 10
Match: AT4G38590.1 (beta-galactosidase 14 )

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-24
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 81  GLNGEKVKAFTQGGSHRVDWSKINEEKTALTWFKNEW----------------------- 140
           GL GEK + +T+ GS +V W K  + K  LTW+K  +                       
Sbjct: 533 GLEGEKKEVYTEEGSKKVKWEKDGKRK-PLTWYKTYFETPEGVNAVAIRMKAMGKGLIWV 592

Query: 141 NGEGI---------DLGE----WYHIPRTFIK--PSENLLVILEEEDHTS-EKVEIVLVN 200
           NG G+          LGE     YHIPR+F+K    +N+LVILEEE     E ++ VLVN
Sbjct: 593 NGIGVGRYWMSFLSPLGEPTQTEYHIPRSFMKGEKKKNMLVILEEEPGVKLESIDFVLVN 652

Query: 201 RNTIYSFITKYHPPNVKSWERKHRQFRAVVDDLTTGAHLQCPDEKKIASIEFVSYGDPSD 239
           R+TI S + + +P +VKSW+R+  +  +   D+   A ++CP EK++  ++F S+GDP+ 
Sbjct: 653 RDTICSNVGEDYPVSVKSWKREGPKIVSRSKDMRLKAVMRCPPEKQMVEVQFASFGDPTG 712

HSP 2 Score: 100.1 bits (248), Expect = 2.8e-21
Identity = 42/69 (60.87%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 12  YHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKKPLLWGTPET 71
           YH G NFGR S  F TTRYYD+APLDE+GL++ PK+GH K VH+AL LCKK L WG    
Sbjct: 239 YHGGTNFGRTSAHFVTTRYYDDAPLDEFGLEKAPKYGHLKHVHRALRLCKKALFWGQLRA 298

Query: 72  QVMGKGLEV 81
           Q +G   EV
Sbjct: 299 QTLGPDTEV 307

[Close]

BLAST of Bhi05G001477 vs. TAIR 10
Match: AT4G38590.2 (beta-galactosidase 14 )

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-24
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 81  GLNGEKVKAFTQGGSHRVDWSKINEEKTALTWFKNEW----------------------- 140
           GL GEK + +T+ GS +V W K  + K  LTW+K  +                       
Sbjct: 597 GLEGEKKEVYTEEGSKKVKWEKDGKRK-PLTWYKTYFETPEGVNAVAIRMKAMGKGLIWV 656

Query: 141 NGEGI---------DLGE----WYHIPRTFIK--PSENLLVILEEEDHTS-EKVEIVLVN 200
           NG G+          LGE     YHIPR+F+K    +N+LVILEEE     E ++ VLVN
Sbjct: 657 NGIGVGRYWMSFLSPLGEPTQTEYHIPRSFMKGEKKKNMLVILEEEPGVKLESIDFVLVN 716

Query: 201 RNTIYSFITKYHPPNVKSWERKHRQFRAVVDDLTTGAHLQCPDEKKIASIEFVSYGDPSD 239
           R+TI S + + +P +VKSW+R+  +  +   D+   A ++CP EK++  ++F S+GDP+ 
Sbjct: 717 RDTICSNVGEDYPVSVKSWKREGPKIVSRSKDMRLKAVMRCPPEKQMVEVQFASFGDPTG 776

HSP 2 Score: 100.1 bits (248), Expect = 2.8e-21
Identity = 42/69 (60.87%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 12  YHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKKPLLWGTPET 71
           YH G NFGR S  F TTRYYD+APLDE+GL++ PK+GH K VH+AL LCKK L WG    
Sbjct: 307 YHGGTNFGRTSAHFVTTRYYDDAPLDEFGLEKAPKYGHLKHVHRALRLCKKALFWGQLRA 366

Query: 72  QVMGKGLEV 81
           Q +G   EV
Sbjct: 367 QTLGPDTEV 375

[Close]

BLAST of Bhi05G001477 vs. TAIR 10
Match: AT1G77410.1 (beta-galactosidase 16 )

HSP 1 Score: 97.1 bits (240), Expect = 2.4e-20
Identity = 67/200 (33.50%), Postives = 93/200 (46.50%), Query Frame = 0


Query: 77  GLEVGLNGEKVKAFTQGGSHRVDWSKINEEKT-ALTWFKNEWN---GE---GIDLGE--- 136
           G +VGL GEK   +T+ GS +V W +  + K+  LTW+K  ++   GE    ++LG    
Sbjct: 574 GYQVGLKGEKFHVYTEDGSAKVQWKQYRDSKSQPLTWYKASFDTPEGEDPVALNLGSMGK 633

Query: 137 ---------------------------WYHIPRTFIKPSENLLVILEEEDHTSE---KVE 196
                                      WYHIPR+F+KP+ NLLVILEEE   +     ++
Sbjct: 634 GEAWVNGQSIGRYWVSFHTYKGNPSQIWYHIPRSFLKPNSNLLVILEEEREGNPLGITID 693

Query: 197 IVLVNRNTIYSFITKYHP---PNVKSWERKHRQFRAVVDDLTTGAHLQCPDEKKIASIEF 234
            V V     +   T  HP   P  K   RK+  +R    D      LQCP  +KI+ I F
Sbjct: 694 TVSVTEVCGHVSNTNPHPVISPRKKGLNRKNLTYRY---DRKPKVQLQCPTGRKISKILF 753

HSP 2 Score: 92.0 bits (227), Expect = 7.6e-19
Identity = 41/77 (53.25%), Postives = 53/77 (68.83%), Query Frame = 0


Query: 1   MATNGTILR-LQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNL 60
           +A NG+ +    YH G NFGR +  F  T YYD+APLDEYGL R+PKWGH K++H A+ L
Sbjct: 281 IAKNGSFVNYYMYHGGTNFGRNASQFVITSYYDQAPLDEYGLLRQPKWGHLKELHAAVKL 340

Query: 61  CKKPLLWGTPETQVMGK 77
           C++PLL G   T  +GK
Sbjct: 341 CEEPLLSGLQTTISLGK 357

[Close]

BLAST of Bhi05G001477 vs. ExPASy Swiss-Prot
Match: Q9SCV1 (Beta-galactosidase 11 OS=Arabidopsis thaliana OX=3702 GN=BGAL11 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 7.6e-32
Identity = 115/498 (23.09%), Postives = 165/498 (33.13%), Query Frame = 0


Query: 4   NGT-ILRLQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NGT +    YH G NFGR S  + TTRYYD+APLDEYGL++EPK+GH K +H ALNLCKK
Sbjct: 300 NGTHVNYYMYHGGTNFGRTSAHYVTTRYYDDAPLDEYGLEKEPKYGHLKHLHNALNLCKK 359

Query: 64  PLLWGTPETQVMGK---------------------------------------------- 123
           PLLWG P+T+  GK                                              
Sbjct: 360 PLLWGQPKTEKPGKDTEIRYYEQPGTKTCAAFLANNNTEAAETIKFKGREYVIAPRSISI 419

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 420 LPDCKTVVYNTAQIVSQHTSRNFMKSKKANKKFDFKVFTETLPSKLEGNSYIPVELYGLT 479

Query: 184 ------------------------------------------------------------ 239
                                                                       
Sbjct: 480 KDKTDYGWYTTSFKVHKNHLPTKKGVKTFVRIASLGHALHAWLNGEYLGSGHGSHEEKSF 539

[Close]

BLAST of Bhi05G001477 vs. ExPASy Swiss-Prot
Match: Q9SCU9 (Beta-galactosidase 13 OS=Arabidopsis thaliana OX=3702 GN=BGAL13 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-29
Identity = 113/493 (22.92%), Postives = 166/493 (33.67%), Query Frame = 0


Query: 4   NGT-ILRLQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NGT +    YH G NFGR S  + TTRYYD+APLDE+GL+REPK+GH K +H ALNLCKK
Sbjct: 303 NGTHVNYYMYHGGTNFGRTSAHYVTTRYYDDAPLDEFGLEREPKYGHLKHLHNALNLCKK 362

Query: 64  PLLWGTP-------ETQVM----------------------------------------- 123
            LLWG P       ET++                                          
Sbjct: 363 ALLWGQPRVEKPSNETEIRYYEQPGTKVCAAFLANNNTEAAEKIKFRGKEYLIPHRSISI 422

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 423 LPDCKTVVYNTGEIISHHTSRNFMKSKKANKNFDFKVFTESVPSKIKGDSFIPVELYGLT 482

Query: 184 -------------------------GK--------------------------------- 234
                                    GK                                 
Sbjct: 483 KDESDYGWYTTSFKIDDNDLSKKKGGKPNLRIASLGHALHVWLNGEYLGNGHGSHEEKSF 542

[Close]

BLAST of Bhi05G001477 vs. ExPASy Swiss-Prot
Match: Q9SCU8 (Beta-galactosidase 14 OS=Arabidopsis thaliana OX=3702 GN=BGAL14 PE=2 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 2.2e-23
Identity = 69/197 (35.03%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 81  GLNGEKVKAFTQGGSHRVDWSKINEEKTALTWFKNEW----------------------- 140
           GL GEK + +T+ GS +V W K  + K  LTW+K  +                       
Sbjct: 603 GLEGEKKEVYTEEGSKKVKWEKDGKRK-PLTWYKTYFETPEGVNAVAIRMKAMGKGLIWV 662

Query: 141 NGEGI---------DLGE----WYHIPRTFIK--PSENLLVILEEEDHTS-EKVEIVLVN 200
           NG G+          LGE     YHIPR+F+K    +N+LVILEEE     E ++ VLVN
Sbjct: 663 NGIGVGRYWMSFLSPLGEPTQTEYHIPRSFMKGEKKKNMLVILEEEPGVKLESIDFVLVN 722

Query: 201 RNTIYSFITKYHPPNVKSWERKHRQFRAVVDDLTTGAHLQCPDEKKIASIEFVSYGDPSD 239
           R+TI S + + +P +VKSW+R+  +  +   D+   A ++CP EK++  ++F S+GDP+ 
Sbjct: 723 RDTICSNVGEDYPVSVKSWKREGPKIVSRSKDMRLKAVMRCPPEKQMVEVQFASFGDPTG 782

HSP 2 Score: 100.1 bits (248), Expect = 3.9e-20
Identity = 42/69 (60.87%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 12  YHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKKPLLWGTPET 71
           YH G NFGR S  F TTRYYD+APLDE+GL++ PK+GH K VH+AL LCKK L WG    
Sbjct: 309 YHGGTNFGRTSAHFVTTRYYDDAPLDEFGLEKAPKYGHLKHVHRALRLCKKALFWGQLRA 368

Query: 72  QVMGKGLEV 81
           Q +G   EV
Sbjct: 369 QTLGPDTEV 377

[Close]

BLAST of Bhi05G001477 vs. ExPASy Swiss-Prot
Match: Q0IZZ8 (Beta-galactosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0539200 PE=2 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 4.6e-21
Identity = 63/204 (30.88%), Postives = 93/204 (45.59%), Query Frame = 0


Query: 67  GTPETQVMGKGLEVGLNGEKVKAFTQGGSHRVDWSKINEEKTALTWFKNEW--------- 126
           GT + Q  G G +  L GE  + +T+ G  +  W K  E    +TW+K  +         
Sbjct: 584 GTLDLQGNGWGHKARLEGEDKEIYTEKGMAQFQW-KPAENDLPITWYKRYFDEPDGDDPI 643

Query: 127 --------------NGEGIDLGEW--------------YHIPRTFIKPSENLLVILEEED 186
                         NGEGI    W              YHIPR F+KP  NLL+I EEE 
Sbjct: 644 VVDMSSMSKGMIYVNGEGIG-RYWTSFITLAGHPSQSVYHIPRAFLKPKGNLLIIFEEEL 703

Query: 187 HTSEKVEIVLVNRNTIYSFITKYHPPNVKSWERKHRQFRAVVDDLTTGAHLQCPDEKKIA 234
                + I  V R+ I  FI++++P  +K+WE    Q + + +D +T   L CP ++ I 
Sbjct: 704 GKPGGILIQTVRRDDICVFISEHNPAQIKTWESDGGQIKLIAEDTSTRGTLNCPPKRTIQ 763

HSP 2 Score: 84.7 bits (208), Expect = 1.7e-15
Identity = 35/79 (44.30%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 2   ATNGTILR-LQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLC 61
           A  GT++    YH G NFGR    +  T YYDEAP+DEYG+ +EPK+GH +D+H  +   
Sbjct: 292 AKGGTLVNYYMYHGGTNFGRTGASYVLTGYYDEAPMDEYGMCKEPKFGHLRDLHNVIKSY 351

Query: 62  KKPLLWGTPETQVMGKGLE 80
            K  LWG    +++G G E
Sbjct: 352 HKAFLWGKQSFEILGHGYE 370

[Close]

BLAST of Bhi05G001477 vs. ExPASy Swiss-Prot
Match: Q6ZJJ0 (Beta-galactosidase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0549200 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.1e-20
Identity = 66/210 (31.43%), Postives = 100/210 (47.62%), Query Frame = 0


Query: 67  GTPETQVMGKGLEVGLNGEKVKAFTQGGSHRVDWSKINEEKTALTWFKNEW--------- 126
           GT +    G G  VGL+GE+ +  ++ G   V W K  ++   LTW++  +         
Sbjct: 583 GTLDLTTNGWGHVVGLDGERRRVHSEQGMGAVAW-KPGKDNQPLTWYRRRFDPPSGTDPV 642

Query: 127 --------------NGEGID---------LGE----WYHIPRTFIKPSENLLVILEEEDH 186
                         NGEG+          LG+     YH+PR+ ++P  N L+  EEE  
Sbjct: 643 VIDLTPMGKGFLFVNGEGLGRYWVSYHHALGKPSQYLYHVPRSLLRPKGNTLMFFEEEGG 702

Query: 187 TSEKVEIVLVNRNTIYSFITKYHPPNVK-SWERKHRQFRAV------VDDLTTGAHLQCP 234
             + + I+ V R+ I +F+T+ +P +V+ SWE K  Q +AV         L   A L CP
Sbjct: 703 KPDAIMILTVKRDNICTFMTEKNPAHVRWSWESKDSQPKAVAGAGAGAGGLKPTAVLSCP 762

HSP 2 Score: 99.4 bits (246), Expect = 6.7e-20
Identity = 41/65 (63.08%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 12  YHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKKPLLWGTPET 71
           YH G NFGR    F   RYYDEAPLDE+GL +EPKWGH +D+H AL  CKK LLWG P  
Sbjct: 301 YHGGTNFGRNGAAFVMPRYYDEAPLDEFGLYKEPKWGHLRDLHHALRHCKKALLWGNPSV 360

Query: 72  QVMGK 77
           Q +GK
Sbjct: 361 QPLGK 365

[Close]

BLAST of Bhi05G001477 vs. ExPASy TrEMBL
Match: A0A5A7U834 (Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00630 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.9e-70
Identity = 180/499 (36.07%), Postives = 206/499 (41.28%), Query Frame = 0


Query: 4   NGTILR-LQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NG+++    YH G NFGR S  FTTTRYYDEAPLDE+GLQREPKWGH +DVHKALNLCKK
Sbjct: 292 NGSLVNYYMYHGGTNFGRTSAVFTTTRYYDEAPLDEFGLQREPKWGHLRDVHKALNLCKK 351

Query: 64  PLLWGTPETQVMGKGLE------------------------------------------- 123
           PLLWGTP TQVMGKGLE                                           
Sbjct: 352 PLLWGTPGTQVMGKGLEARFYEKPGTNICAAFLANNDTKNAQTINFRGREYLLPPRSISI 411

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 412 LPDCKTVVFNTETIVSQHNARNFVPSKVANNLKWKMSPETIPTVQQVPVNNKIPLELYSL 471

Query: 184 ------------------------------------------------------------ 241
                                                                       
Sbjct: 472 LKDTTDYGWYTTSIELEKEDVSKRPDILPVLRIASLGHAILVFVNGEYIGTAHGSHEEKN 531

[Close]

BLAST of Bhi05G001477 vs. ExPASy TrEMBL
Match: A0A1S3BIM9 (Beta-galactosidase OS=Cucumis melo OX=3656 GN=LOC103490291 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.9e-70
Identity = 180/499 (36.07%), Postives = 206/499 (41.28%), Query Frame = 0


Query: 4   NGTILR-LQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NG+++    YH G NFGR S  FTTTRYYDEAPLDE+GLQREPKWGH +DVHKALNLCKK
Sbjct: 292 NGSLVNYYMYHGGTNFGRTSAVFTTTRYYDEAPLDEFGLQREPKWGHLRDVHKALNLCKK 351

Query: 64  PLLWGTPETQVMGKGLE------------------------------------------- 123
           PLLWGTP TQVMGKGLE                                           
Sbjct: 352 PLLWGTPGTQVMGKGLEARFYEKPGTNICAAFLANNDTKNAQTINFRGREYLLPPRSISI 411

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 412 LPDCKTVVFNTETIVSQHNARNFVPSKVANNLKWKMSPETIPTVQQVPVNNKIPLELYSL 471

Query: 184 ------------------------------------------------------------ 241
                                                                       
Sbjct: 472 LKDTTDYGWYTTSIELEKEDVSKRPDILPVLRIASLGHAILVFVNGEYIGTAHGSHEEKN 531

[Close]

BLAST of Bhi05G001477 vs. ExPASy TrEMBL
Match: A0A0A0K5Y3 (Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_7G011860 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 6.2e-69
Identity = 178/499 (35.67%), Postives = 204/499 (40.88%), Query Frame = 0


Query: 4   NGTILR-LQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NG+++    YH G NFGR S  FTTTRYYDEAPLDE+GLQREPKWGH +DVHKALNLCKK
Sbjct: 292 NGSLVNYYMYHGGTNFGRTSAVFTTTRYYDEAPLDEFGLQREPKWGHLRDVHKALNLCKK 351

Query: 64  PLLWGTPETQVMGKGLE------------------------------------------- 123
           PLLWGTP  QV+GKGLE                                           
Sbjct: 352 PLLWGTPGIQVIGKGLEARFYEKPGTNICAAFLANNDTKSAQTINFRGREFLLPPRSISI 411

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 412 LPDCKTVVFNTETIVSQHNARNFIPSKNANKLKWKMSPESIPTVEQVPVNNKIPLELYSL 471

Query: 184 ------------------------------------------------------------ 241
                                                                       
Sbjct: 472 LKDTTDYGWYTTSIELDKEDVSKRPDILPVLRIASLGHAMLVFVNGEYIGTAHGSHEEKN 531

[Close]

BLAST of Bhi05G001477 vs. ExPASy TrEMBL
Match: A0A6J1HWJ1 (Beta-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111468497 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 9.3e-65
Identity = 166/499 (33.27%), Postives = 203/499 (40.68%), Query Frame = 0


Query: 4   NGTILR-LQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NG+++    YH G NFGR S  FTTTRYYDEAPLDE+GLQREPKWGH +DVHKALNLCKK
Sbjct: 287 NGSLVNYYMYHGGTNFGRTSAVFTTTRYYDEAPLDEFGLQREPKWGHLRDVHKALNLCKK 346

Query: 64  PLLWGTPETQVMGKGLE------------------------------------------- 123
           PLLWGTP TQVMG+GLE                                           
Sbjct: 347 PLLWGTPRTQVMGQGLEARIYEKPGTNICAAFLANNDTKFAQTVNFGGRDYLLPPRSISV 406

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 407 LPDCTTVVYNTQTIVAQHNARNFVPSKVATNFQWKMYSEPIPTVEQIPVNNKIPLELYTL 466

Query: 184 ------------------------------------------------------------ 241
                                                                       
Sbjct: 467 LKDTTDYGWYTTSIELDIEDVSKRPDILPVIRIASLGHAMLVYVNGEYIGGAHGSHEEKN 526

[Close]

BLAST of Bhi05G001477 vs. ExPASy TrEMBL
Match: A0A6J1GKJ1 (Beta-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111455157 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 1.2e-64
Identity = 167/499 (33.47%), Postives = 202/499 (40.48%), Query Frame = 0


Query: 4   NGTILR-LQYHSGPNFGRISVFFTTTRYYDEAPLDEYGLQREPKWGHFKDVHKALNLCKK 63
           NG+++    YH G NFGR S  FTTTRYYDEAPLDE+GLQREPKWGH +DVHKALNLCKK
Sbjct: 287 NGSLVNYYMYHGGTNFGRTSAVFTTTRYYDEAPLDEFGLQREPKWGHLRDVHKALNLCKK 346

Query: 64  PLLWGTPETQVMGKGLE------------------------------------------- 123
           PLLWGTP TQVMGKGLE                                           
Sbjct: 347 PLLWGTPRTQVMGKGLEARIYEKPGTNICAAFLANNDTKFAQTVNFGGRDYLLPPRSISV 406

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 407 LPDCTTVVYNTQTIVSQHNARNFVPSKVATNFQWKMYSEPVPTVEHVPVNNKIPLELYTL 466

Query: 184 ------------------------------------------------------------ 241
                                                                       
Sbjct: 467 LKDTTDYGWYTTSIELDIEDVSKRPDILPVIRIASLGHAMLVYVNGEYIGGAHGSHEEKN 526

The following BLAST results are available for this feature:

BLAST of Bhi05G001477 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35010.1	5.4e-33	23.09	beta-galactosidase 11	[more]
AT2G16730.1	1.5e-30	22.92	glycosyl hydrolase family 35 protein	[more]
AT4G38590.1	1.6e-24	35.03	beta-galactosidase 14	[more]
AT4G38590.2	1.6e-24	35.03	beta-galactosidase 14	[more]
AT1G77410.1	2.4e-20	33.50	beta-galactosidase 16	[more]

BLAST of Bhi05G001477 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCV1	7.6e-32	23.09	Beta-galactosidase 11 OS=Arabidopsis thaliana OX=3702 GN=BGAL11 PE=2 SV=1	[more]
Q9SCU9	2.1e-29	22.92	Beta-galactosidase 13 OS=Arabidopsis thaliana OX=3702 GN=BGAL13 PE=2 SV=1	[more]
Q9SCU8	2.2e-23	35.03	Beta-galactosidase 14 OS=Arabidopsis thaliana OX=3702 GN=BGAL14 PE=2 SV=2	[more]
Q0IZZ8	4.6e-21	30.88	Beta-galactosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0539200 P...	[more]
Q6ZJJ0	5.1e-20	31.43	Beta-galactosidase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0549200 P...	[more]

BLAST of Bhi05G001477 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U834	1.9e-70	36.07	Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G0...	[more]
A0A1S3BIM9	1.9e-70	36.07	Beta-galactosidase OS=Cucumis melo OX=3656 GN=LOC103490291 PE=3 SV=1	[more]
A0A0A0K5Y3	6.2e-69	35.67	Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_7G011860 PE=3 SV=1	[more]
A0A6J1HWJ1	9.3e-65	33.27	Beta-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111468497 PE=3 SV=1	[more]
A0A6J1GKJ1	1.2e-64	33.47	Beta-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111455157 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 2..60 e-value: 9.2E-6 score: 27.2
None	No IPR available	PANTHER	PTHR23421:SF52	BETA-GALACTOSIDASE 14	coord: 127..238 coord: 4..80
None	No IPR available	PANTHER	PTHR23421:SF52	BETA-GALACTOSIDASE 14	coord: 79..118
IPR031330	Glycoside hydrolase 35, catalytic domain	PFAM	PF01301	Glyco_hydro_35	coord: 12..56 e-value: 5.1E-7 score: 29.6
IPR001944	Glycoside hydrolase, family 35	PANTHER	PTHR23421	BETA-GALACTOSIDASE RELATED	coord: 79..118
IPR001944	Glycoside hydrolase, family 35	PANTHER	PTHR23421	BETA-GALACTOSIDASE RELATED	coord: 4..80
IPR001944	Glycoside hydrolase, family 35	PANTHER	PTHR23421	BETA-GALACTOSIDASE RELATED	coord: 127..238
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 12..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M001477	Bhi05M001477	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds