Bhi05G001426 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G001426
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	O-methyltransferase family protein
Location	chr5: 54126604 .. 54129400 (-)
RNA-Seq Expression	Bhi05G001426
Synteny	Bhi05G001426

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGGTCATTCCATTGATCGAAACTCATTTTGGTACTATCTTCCTTCGTTACTATATATATATGGGAACATTAGAATAAGAACTTGCAAGAACAAGTCAAGACAATATCGATCGATCATGTAAATAATGGATGAAAATGGTAGCAAGGAGTCCAATAAATTGAAGATAGAAGAAGAAGAGAGTGATTTGAAAGTAGAAGTATGGAAATACATATTTGGTTTCGTTGAAATGGCAGTAATAAAATGTGCGATTGAGCTCAAAATAGGAGACACCATTGAAAGCAATGGAGGTTCCATGACACTCTCCGAGCTATCTTCTTCTTTAAACTCTTCTCCTTTGCTTCTACACCGAGTCTTGCGGTTCCTTGTTCATCGAGGCATTTTCAAAGAAGAAAAAGCCACACAAAGCTACTCTCAAACGCCCATGTCTCGGCTGCTCACAACCACTGGACCAAATAGCTTGGCTCCCATACATCTCTTAGAGAGCAGCCCTGCGATGGTTGCACCATGGCACTACTTGAGTGCATGTCTTAAAGCCAATGATCATGAGAATGAGAGCCAGCCTTTTGAGTTGGCTCATGGGATGGACCTGTGGAGTTATGCTTCAGCCAATCCATCGCACAACAAGTTGGTGAATGAGGCCATGGCTTGCTTTGCTAGGGTGGTCATTTTGCCTGCAGTGCTTCAAAGGTCTCATAATATCCACCCGAGTTTCAGATAAATTAAGATTAAAATGATAGAACTAGTATTTTTGGCTCCCGTTTACATTTTTTTAAAAATTATTTTAGTGATGTAATTTTTTTATTTAGAAAATTTATCAAATATTCAACTAGTTTTTGCTTTGAAATTTACATGCATTTAGTAATGATTTTAGATAATAATCTGAGTAATTCGGATGTTTACAGGTGTGGGGAGATTTTTGATGGAGTTGGAAGTTTGGTTGATGTTGGAGGAGGAAATGGGACATGTTTGAGTATTTTAGTGAAGGCTTGTCCTTGGATTAAAGGCATTAATTTTGATCTTCCTCATGTTGTGTCTGGTTCACAAGAGTATGAAGGTGTTCAACATGTTGGTGGCAATATGTTTGATTCTATCCCTAAGGCTGATGCTGCTTTCATCATGGTACGTTATTTAATTTGGGTGTGTTTGTGGATGGTAAATTTGTTACTTGATATTGGTCTGATCTCAAAACTAATTATGACAATAAGTAGAGTAACTCATGTATCTTATAAAGATGGTAAGATCTCTTGATATATCTAATATTTTAAGGTTTTAAGTTTTGTTTAATTTAGTACAACTTTTAAGTTGGCCTTAAAGTTTCTTTAACAATCATTTTGTTTTTTTGTTTTTTAAAATGGTATTTATTTTCATACAAATTTGGTATAGTGGTTTTTTTTTTTTTTTAACTCTTAATCAAATTCCAAGCCTTAACCAAGAAAATTTAACTTCGCTTGGATATTGAAAATATTATAAAATATAGAAAACAAAATCAATAAATCCAAATGAAATTACATTGCTTATAATTTTTAAAAGTAAAATGGTTACTACACAAGGTTCAAATTTTCATGTCGGTTCTATTTTAGACTCTAAATTTTTAAGACGGTTTATTTTTGTCCTTAAACTTTTCAAGTTTATTGTAATTAGTTTTTAACTTTCAAAATATTTGAATCCTTAAAAAAACAATCATTTTGGTCATCTTTACTATGTTACATTTTTATCTTGAAATTTGTTGTTGAATGTTTGTCTCTTACATGTTTTATTTGAATCATGACATGGTAACATCCTAGGGCATAGGCACCAGAAGTTACTTCATTAATTTGTGAAATAAAAAAAATAAAAGTAAAAAATCAAAATAGCTAGTTATTTTTTAAAAGTTCAGATATTAAAATAAATATTTTAAAAGTCTAAGCACCAAAATAGAACAACCTAAAAAGCTTAAGAGCAGTTTAGTATTTTAATAAGCAAAACACACACATAAATATGCATTATTTGGAAGTCTACTATAATTTTGGGACAGTTGCAAATATAGTAATGAAGTCCAAAATATTTGTAAATCTAGCACAATGCAAAATTTTTTATAGATATAGCAAAATTTATATTTTACTTTCGAAATTTATCGGTAATAGACCATATTGGTAGTTAGTGTAAATCAGTGATAGATCATATCGTAGATAGAAGTCTATTAACAACAGAGCCTATCATTTATATATTTTACTATATTTGTAATTTTTTAAAAATGTTCCACTTAATTATTATTTCCATACAATTATCTTATAATTTTTATTGTTTTGAGGCTCACTAACAACAAATAATGCAACAAATATCATTGCAGGAGGTTCTACATGATTGGGATGATGAAGAATGCATAAAAATTTTGAAAAATGTCAAAGAGGCCATTCCTAAAAAGACGGGAAAAGTGATAATTGTTGAAGTGGTGATTGATGAAAATGAAAAAATTAGCCAATTATCAGATATAAGGTTAATGATGGATATGATAATGATGGCTCATACCATTAAGGGTAAAGAAAGAACAAATGTGGAATGGGCCAATGTTCTTCAAAAGGCTGGTTTTAGTCACTATACAATCACTCCTACTACAAGCATCCAATCTGTTATTCAAGCTTTTCCTTGATATCAATCAAATAAATTTCATGTATTGGTAATTATTGCTTCTATTTCATGTCATATTTCATTATATTGCTGATCTGTCTTTTTATTTTAAAAGTTTGTAATAATTGATTCAAAGAACAGGTATGTTTCTATTAAATACAAAATTGTGTTGAATACTTTTTTT

mRNA sequence

ATGAAAAGGTCATTCCATTGATCGAAACTCATTTTGGTACTATCTTCCTTCGTTACTATATATATATGGGAACATTAGAATAAGAACTTGCAAGAACAAGTCAAGACAATATCGATCGATCATGTAAATAATGGATGAAAATGGTAGCAAGGAGTCCAATAAATTGAAGATAGAAGAAGAAGAGAGTGATTTGAAAGTAGAAGTATGGAAATACATATTTGGTTTCGTTGAAATGGCAGTAATAAAATGTGCGATTGAGCTCAAAATAGGAGACACCATTGAAAGCAATGGAGGTTCCATGACACTCTCCGAGCTATCTTCTTCTTTAAACTCTTCTCCTTTGCTTCTACACCGAGTCTTGCGGTTCCTTGTTCATCGAGGCATTTTCAAAGAAGAAAAAGCCACACAAAGCTACTCTCAAACGCCCATGTCTCGGCTGCTCACAACCACTGGACCAAATAGCTTGGCTCCCATACATCTCTTAGAGAGCAGCCCTGCGATGGTTGCACCATGGCACTACTTGAGTGCATGTCTTAAAGCCAATGATCATGAGAATGAGAGCCAGCCTTTTGAGTTGGCTCATGGGATGGACCTGTGGAGTTATGCTTCAGCCAATCCATCGCACAACAAGTTGGTGAATGAGGCCATGGCTTGCTTTGCTAGGGTGGTCATTTTGCCTGCAGTGCTTCAAAGGTGTGGGGAGATTTTTGATGGAGTTGGAAGTTTGGTTGATGTTGGAGGAGGAAATGGGACATGTTTGAGTATTTTAGTGAAGGCTTGTCCTTGGATTAAAGGCATTAATTTTGATCTTCCTCATGTTGTGTCTGGTTCACAAGAGTATGAAGGTGTTCAACATGTTGGTGGCAATATGTTTGATTCTATCCCTAAGGCTGATGCTGCTTTCATCATGGAGGTTCTACATGATTGGGATGATGAAGAATGCATAAAAATTTTGAAAAATGTCAAAGAGGCCATTCCTAAAAAGACGGGAAAAGTGATAATTGTTGAAGTGGTGATTGATGAAAATGAAAAAATTAGCCAATTATCAGATATAAGGTTAATGATGGATATGATAATGATGGCTCATACCATTAAGGGTAAAGAAAGAACAAATGTGGAATGGGCCAATGTTCTTCAAAAGGCTGGTTTTAGTCACTATACAATCACTCCTACTACAAGCATCCAATCTGTTATTCAAGCTTTTCCTTGATATCAATCAAATAAATTTCATGTATTGGTAATTATTGCTTCTATTTCATGTCATATTTCATTATATTGCTGATCTGTCTTTTTATTTTAAAAGTTTGTAATAATTGATTCAAAGAACAGGTATGTTTCTATTAAATACAAAATTGTGTTGAATACTTTTTTT

Coding sequence (CDS)

ATGGATGAAAATGGTAGCAAGGAGTCCAATAAATTGAAGATAGAAGAAGAAGAGAGTGATTTGAAAGTAGAAGTATGGAAATACATATTTGGTTTCGTTGAAATGGCAGTAATAAAATGTGCGATTGAGCTCAAAATAGGAGACACCATTGAAAGCAATGGAGGTTCCATGACACTCTCCGAGCTATCTTCTTCTTTAAACTCTTCTCCTTTGCTTCTACACCGAGTCTTGCGGTTCCTTGTTCATCGAGGCATTTTCAAAGAAGAAAAAGCCACACAAAGCTACTCTCAAACGCCCATGTCTCGGCTGCTCACAACCACTGGACCAAATAGCTTGGCTCCCATACATCTCTTAGAGAGCAGCCCTGCGATGGTTGCACCATGGCACTACTTGAGTGCATGTCTTAAAGCCAATGATCATGAGAATGAGAGCCAGCCTTTTGAGTTGGCTCATGGGATGGACCTGTGGAGTTATGCTTCAGCCAATCCATCGCACAACAAGTTGGTGAATGAGGCCATGGCTTGCTTTGCTAGGGTGGTCATTTTGCCTGCAGTGCTTCAAAGGTGTGGGGAGATTTTTGATGGAGTTGGAAGTTTGGTTGATGTTGGAGGAGGAAATGGGACATGTTTGAGTATTTTAGTGAAGGCTTGTCCTTGGATTAAAGGCATTAATTTTGATCTTCCTCATGTTGTGTCTGGTTCACAAGAGTATGAAGGTGTTCAACATGTTGGTGGCAATATGTTTGATTCTATCCCTAAGGCTGATGCTGCTTTCATCATGGAGGTTCTACATGATTGGGATGATGAAGAATGCATAAAAATTTTGAAAAATGTCAAAGAGGCCATTCCTAAAAAGACGGGAAAAGTGATAATTGTTGAAGTGGTGATTGATGAAAATGAAAAAATTAGCCAATTATCAGATATAAGGTTAATGATGGATATGATAATGATGGCTCATACCATTAAGGGTAAAGAAAGAACAAATGTGGAATGGGCCAATGTTCTTCAAAAGGCTGGTTTTAGTCACTATACAATCACTCCTACTACAAGCATCCAATCTGTTATTCAAGCTTTTCCTTGA

Protein sequence

MDENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLSELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLESSPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVVILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGVQHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENEKISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP

Homology

[Close]

BLAST of Bhi05G001426 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 342.8 bits (878), Expect = 3.3e-94
Identity = 180/371 (48.52%), Postives = 250/371 (67.39%), Query Frame = 0


Query: 3   ENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGS--MTLS 62
           +    E NK K+ +EE+   +++WKY+FGF ++A  KCAI+LKI + IE++  S  +TL+
Sbjct: 11  DRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLA 70

Query: 63  ELSSSLNSSPLLLHRVLRFLVHRGIFKE----EKATQSYSQTPMSR--LLTTTGPNSLAP 122
           ELSS++++SP  L R++RFLVH+GIFKE    +     Y  TP+SR  ++T     SLAP
Sbjct: 71  ELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAP 130

Query: 123 IHLLESSPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMA 182
             L E++P M+APW  LS+ + +  + +   PF+  HG D+WS+A  NP  + ++NEAMA
Sbjct: 131 FVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMA 190

Query: 183 CFARVVILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGS 242
           C AR V+ P V   C  +FDGV ++VDVGGG G  + +LVK  PWIKG NFDLPHV+  +
Sbjct: 191 CDARRVV-PRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 250

Query: 243 QEYEGVQHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEV 302
           +  +GV++V G+MFDSIP  DA FI  VLHDW D++CIKILKN KEA+P   GKV+IVE 
Sbjct: 251 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 310

Query: 303 VIDENEKI-------SQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITP 359
           VI EN+K         +L  +RLM+DM+MMAHT  GKERT  EW  VL++AGF+ Y +  
Sbjct: 311 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 370

[Close]

BLAST of Bhi05G001426 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 288.1 bits (736), Expect = 9.7e-78
Identity = 160/364 (43.96%), Postives = 220/364 (60.44%), Query Frame = 0


Query: 8   ESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGS--MTLSELSSS 67
           E +K  + +EE+   +++W+Y+FGF ++A  KCAI+LKI + IE++  S  +TLSELSS+
Sbjct: 2   EESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSA 61

Query: 68  LNSSPLLLHRVLRFLVHRGIFKE----EKATQSYSQTPMSRLLTTTGPNSLAPIHLLESS 127
           +++SP  L R++RFLVH+G+FKE    +     Y+ TP+SR +  T              
Sbjct: 62  VSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMIT-------------- 121

Query: 128 PAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVVI 187
                                        HG DLW++A  N  H++L+NEAMAC AR V+
Sbjct: 122 ---------------------------KLHGKDLWAFAQDNLCHSQLINEAMACDARRVV 181

Query: 188 LPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGVQ 247
            P V   C  +FDGV ++VDVGGG G  + ILVK  PWIKG NFDLPHV+  +Q  +GV+
Sbjct: 182 -PRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVE 241

Query: 248 HVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDE--- 307
           +V G+MFDSIP +DA  I  VLHDW D++CIKILKN KEA+    GKV+IVE VI E   
Sbjct: 242 NVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKN 301

Query: 308 ----NEKISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSV 359
                E+  +L  +RL +DM+MM HT  GKERT  EW  VL +AGF+ Y +     +QS+
Sbjct: 302 TMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSL 323

[Close]

BLAST of Bhi05G001426 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 180.6 bits (457), Expect = 2.2e-45
Identity = 106/315 (33.65%), Postives = 172/315 (54.60%), Query Frame = 0


Query: 38  IKCAIELKIGDTIESNGGSMTLSELSSSLNS----SPLLLHRVLRFLVHRGI-------F 97
           +K A+EL + + +  NG  M+ +E++S L +    +P++L R+LR L    +        
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 98  KEEKATQSYSQTPMSRLLTTTGPN-SLAPIHLLESSPAMVAPWHYLSACLKANDHENESQ 157
             +   + Y   P+ + LT      S+A + L+     ++  W++L   +      +   
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAIL-----DGGI 155

Query: 158 PFELAHGMDLWSYASANPSHNKLVNEAMACFARVVILPAVLQRCGEIFDGVGSLVDVGGG 217
           PF  A+GM  + Y   +P  NK+ N  M+  + + +   +    G  F+G+ SLVDVGGG
Sbjct: 156 PFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG--FEGLTSLVDVGGG 215

Query: 218 NGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGVQHVGGNMFDSIPKADAAFIMEVLHD 277
            G  L ++V   P +KGINFDLPHV+  +  + G++HVGG+MF S+PK DA F+  + HD
Sbjct: 216 IGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHD 275

Query: 278 WDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENEKISQLSDIRLMMDMIMMAHTIKGKE 337
           W DE C+K LKN  E++P + GKVI+ E ++ E    S  +   + +D IM+AH   GKE
Sbjct: 276 WSDEHCVKFLKNCYESLP-EDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKE 335

Query: 338 RTNVEWANVLQKAGF 341
           RT  E+  + + +GF
Sbjct: 336 RTEKEFEALAKASGF 342

[Close]

BLAST of Bhi05G001426 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 130/368 (35.33%), Postives = 198/368 (53.80%), Query Frame = 0


Query: 15  EEEESDLK---VEVWKYIFGFVEMAVIKCAIELKIGDTI--ESNGGSMTLSELSSSLNS- 74
           EE+E D K   ++    +       V+K A+EL + DTI   SNG  ++ SE++ SL + 
Sbjct: 20  EEQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNK 79

Query: 75  -----SPLLLHRVLRFLVHRGIFK-----------EEKATQSYSQTPMSR--LLTTTGPN 134
                +P+LL R+LR LV   I K             K  + Y+  P+ +  L  + G  
Sbjct: 80  PTNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSG 139

Query: 135 SLAPIHLLESSPAMVAPWHYL-SACLKANDHENESQPFELAHGMDLWSYASANPSHNKLV 194
           SL+ + LL  S  ++  W  L    L+  D       F  AH M L+ Y S++   +KL 
Sbjct: 140 SLSSLLLLLHSQVILKTWTNLKDVILEGKD------AFSSAHDMRLFEYISSDDQFSKLF 199

Query: 195 NEAMACFARVVILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPH 254
           + AM+  + +V+   + +  G  F+ V +LVDVGGG GT L ++    P IKG+NFDL  
Sbjct: 200 HRAMSESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQ 259

Query: 255 VVSGSQEYEGVQHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKV 314
           V++ +  Y GV+HV G+MF  +PK DA F+  +LHDW DE+CIKILKN  +++P+K GKV
Sbjct: 260 VLTQAPFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GKV 319

Query: 315 IIVEVVIDENEKISQLS-DIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPT 357
           IIVE++     K +  S +  L MD++M+     GKER+  ++ N+   +GF    I   
Sbjct: 320 IIVEMITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICL 378

[Close]

BLAST of Bhi05G001426 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 170.2 bits (430), Expect = 2.9e-42
Identity = 121/343 (35.28%), Postives = 181/343 (52.77%), Query Frame = 0


Query: 37  VIKCAIELKIGDTIESNGGSMTLS--ELSSSLNS------SPLLLHRVLRFLVHRGIFK- 96
           V+K A EL + DTI + G    LS  E++ SL +      +P+LL R+L  LV   I K 
Sbjct: 45  VLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILKC 104

Query: 97  ----------EEKATQSYSQTPMSR--LLTTTGPNSLAPIHLLESSPAMVAPWHYL-SAC 156
                       K  + Y+  P+ +  L  + G  SL P+ +L  +      W  L    
Sbjct: 105 RMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDVI 164

Query: 157 LKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVVILPAVLQRCGEIFD 216
           L+  D       F  AHGM ++ Y +++    +L N AM+  + +++   +    G  F+
Sbjct: 165 LEGRD------AFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYRG--FE 224

Query: 217 GVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGVQHVGGNMFDSIPKA 276
            V +LVDVGGGNGT L ++    P IKG+NFDL  V++ +  Y GV+HV G+MF  +PK 
Sbjct: 225 DVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKG 284

Query: 277 DAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENEKISQLSDIRLM-MD 336
           DA F+  +LHDW DE+CIKILKN  +++P+K GK+IIVE V  +  K   LS   +  MD
Sbjct: 285 DAVFMKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMD 344

Query: 337 MIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQ 357
           ++M+     GKER+  ++ N+   +GF    I       SVI+
Sbjct: 345 LLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

[Close]

BLAST of Bhi05G001426 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 4.6e-93
Identity = 180/371 (48.52%), Postives = 250/371 (67.39%), Query Frame = 0


Query: 3   ENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGS--MTLS 62
           +    E NK K+ +EE+   +++WKY+FGF ++A  KCAI+LKI + IE++  S  +TL+
Sbjct: 11  DRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLA 70

Query: 63  ELSSSLNSSPLLLHRVLRFLVHRGIFKE----EKATQSYSQTPMSR--LLTTTGPNSLAP 122
           ELSS++++SP  L R++RFLVH+GIFKE    +     Y  TP+SR  ++T     SLAP
Sbjct: 71  ELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAP 130

Query: 123 IHLLESSPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMA 182
             L E++P M+APW  LS+ + +  + +   PF+  HG D+WS+A  NP  + ++NEAMA
Sbjct: 131 FVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMA 190

Query: 183 CFARVVILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGS 242
           C AR V+ P V   C  +FDGV ++VDVGGG G  + +LVK  PWIKG NFDLPHV+  +
Sbjct: 191 CDARRVV-PRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 250

Query: 243 QEYEGVQHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEV 302
           +  +GV++V G+MFDSIP  DA FI  VLHDW D++CIKILKN KEA+P   GKV+IVE 
Sbjct: 251 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 310

Query: 303 VIDENEKI-------SQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITP 359
           VI EN+K         +L  +RLM+DM+MMAHT  GKERT  EW  VL++AGF+ Y +  
Sbjct: 311 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 370

[Close]

BLAST of Bhi05G001426 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-71
Identity = 153/358 (42.74%), Postives = 217/358 (60.61%), Query Frame = 0


Query: 16  EEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLSELSSSL-----NSSP 75
           EE    + E+W+++F FV+   +KCA+EL I D I S+G  +T+SE+  SL     +SSP
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 76  LL--LHRVLRFLVHRGIFKEEKATQS----YSQTPMSRLLTTTGPNSLAPIHLLESSPAM 135
            +  L R++R LVH+ +F  E   +S    Y+ T  S+ L      +L+P+ L E++P +
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 136 VAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVVILPA 195
           + PW YL  C      + +S PFE AHG ++W  A A+P  N  +N AM C    +I   
Sbjct: 125 LKPWQYLGKCA-----QEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEM 184

Query: 196 VLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGVQHVG 255
           +L+         GSLVDVGGG G+ ++ +VKA P I+GINFDLPHVV+ + E+ GV+HVG
Sbjct: 185 LLEYKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVG 244

Query: 256 GNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAI-PKKTGKVIIVEVVI--DENEK 315
           G+MF  IP+ADA  +  +LHDW DE+C  ILKN   AI  KK GKVIIV+ V+  D N+ 
Sbjct: 245 GDMFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDL 304

Query: 316 ISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP 360
             ++    L+ D++MMAHT  GKERT  EW  +L  AGF  Y +  T +   +I+AFP
Sbjct: 305 FDKMG---LIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

[Close]

BLAST of Bhi05G001426 vs. ExPASy Swiss-Prot
Match: Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 6.1e-69
Identity = 152/352 (43.18%), Postives = 216/352 (61.36%), Query Frame = 0


Query: 13  KIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLSELSSSLN-SSPL 72
           KI+++    + ++WK I+GF E  V+KCA++L+I +T+ +N   M+LSEL+S L  + P+
Sbjct: 6   KIDQQN---QAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPV 65

Query: 73  ---LLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLESSPAMVAPW 132
               L R++R+LVH  +FK +  TQ YS  P ++ L      S+    L  +    +APW
Sbjct: 66  NEDRLFRIMRYLVHMELFKIDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPW 125

Query: 133 HYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVVILPAVLQR 192
           H+L   L  N        FE A G  +W Y S NP  N+L N AMAC  R+V   A+   
Sbjct: 126 HHLGDGLTGN-----CDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLV-TSALANE 185

Query: 193 CGEIF-DGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGVQHVGGNM 252
           C  IF DG+ +LVDVGGG GT +  + KA P IK   +DLPHV++ S E   +  + G+M
Sbjct: 186 CKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDM 245

Query: 253 FDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENEKISQLSD 312
           F SIP ADA F+  +LHDW+D+ECI+ILK  KEA+P K GKVIIV+VVID  +     + 
Sbjct: 246 FKSIPSADAIFMKCILHDWNDDECIQILKRCKEALP-KGGKVIIVDVVID-MDSTHPYAK 305

Query: 313 IRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP 360
           IRL +D+ MM +T  GKERT  EW  +   AGF+ + +T  +++QSVI+A+P
Sbjct: 306 IRLTLDLDMMLNT-GGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345

[Close]

BLAST of Bhi05G001426 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.0e-68
Identity = 144/353 (40.79%), Postives = 216/353 (61.19%), Query Frame = 0


Query: 12  LKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLSELSSSLNSSPL 71
           ++++++    + ++W +I+GF E  V+KCA++L + + I ++G SMTLSELSS L S P+
Sbjct: 1   MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPV 60

Query: 72  ---LLHRVLRFLVHRGIFKEEK--ATQSYSQTPMSRLLTTTGPNSLAPIHLLESSPAMVA 131
               L+RV+R+LVH  +F +        Y   P ++ L       +    L  +    +A
Sbjct: 61  NEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 120

Query: 132 PWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVVILPAVL 191
           PWHYL   L           FE A G ++W Y + +P  N+L NEAMA  +R +I+ A++
Sbjct: 121 PWHYLKDGLSG----ESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSR-LIMSALV 180

Query: 192 QRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGVQHVGGN 251
           + CG IF+G+ +LVDVGGG GT +  +  A P IK   +DLPHV++ S  Y  V  V G+
Sbjct: 181 KECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGD 240

Query: 252 MFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENEKISQLS 311
           MF  IPKADA  +  +LHDWDD+ECI+ILK  KEA+P K GKVIIV++V++   +    +
Sbjct: 241 MFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSE-HPYT 300

Query: 312 DIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP 360
            +RL +D+ MM +T  GKERT  EW  ++  AG+  + IT  T++QSVI+A+P
Sbjct: 301 KMRLTLDLDMMLNT-GGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

[Close]

BLAST of Bhi05G001426 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.2e-68
Identity = 156/361 (43.21%), Postives = 221/361 (61.22%), Query Frame = 0


Query: 15  EEEESDLKVE--VWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLSELSSSLNSSPLL 74
           ++ E+ L+ E  VWK I G  +  V+KCA+EL+I D + S+   +TL++++SS+  SP L
Sbjct: 7   DQTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDSPSL 66

Query: 75  ----LHRVLRFLVHRGIFKEEKATQS----YSQTPMSRLL--TTTGPN--SLAPIHLLES 134
               L R++R LV R IF + K+       Y  T  SRLL   TT P+  +LAP     +
Sbjct: 67  NLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMT 126

Query: 135 SPAMVAPWHYLSACLKANDHENESQPFELAH-GMDLWSYASANPSHNKLVNEAMACFARV 194
            P + APW  L+ C+K          FE+ H G  LW  +  NP  NK+ N+ MA  AR+
Sbjct: 127 HPYLSAPWSCLARCVKEG-----GNGFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARI 186

Query: 195 VILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEG 254
             + A+L    ++F G+ SLVDVGG  G  +S +VK+ P IKGIN+DLPHVV+ +  Y G
Sbjct: 187 TTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYTG 246

Query: 255 -VQHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDE 314
            V HVGGNMF+ IP A A F+  +LHDW DE+C+KILKN + A+P+K GK+IIV++V+ E
Sbjct: 247 LVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVL-E 306

Query: 315 NEKISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAF 360
            E      D  +M+D+ +MA T +GKERT  EW  VL++ GF  Y I    ++ SVI+A+
Sbjct: 307 PEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAY 359

[Close]

BLAST of Bhi05G001426 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.2e-178
Identity = 308/359 (85.79%), Postives = 339/359 (94.43%), Query Frame = 0


Query: 1   MDENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLS 60
           MD+  SK SNKLKIEEE+SD+KV++WKYIFGFVEMAVIKCAIELKIGD IES+G SMTLS
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLS 60

Query: 61  ELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLES 120
           +LSSSLN SPLLLHR+LRFLVHRGIFKEEK T+ YS TPMSRLLTTTGPNS+AP+HLLES
Sbjct: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTKCYSHTPMSRLLTTTGPNSMAPLHLLES 120

Query: 121 SPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVV 180
           SP M+APWH LSAC+KA+DHEN SQPFE+AHGMDLW+YA+ANPSH+ L+NE+MACFAR+V
Sbjct: 121 SPVMIAPWHNLSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLV 180

Query: 181 ILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGV 240
           ILPAVL+RCGEIFDGVG LVDVGGGNGTCLSILVKACPWIKGINFDLPHVVS S+EYEG+
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGI 240

Query: 241 QHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENE 300
           QHVGGNMFDS+PKADAAFIMEVLHDWDDEECIKILKN KEAIP+KTGKVIIVEVVIDE E
Sbjct: 241 QHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKE 300

Query: 301 KISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP 360
           +IS+ SD RLMMDMIMMAHTIKGKERTN EWANVLQKAGFS+YTITPT++IQSVIQAFP
Sbjct: 301 EISKYSDARLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359

[Close]

BLAST of Bhi05G001426 vs. ExPASy TrEMBL
Match: A0A1S3BHY1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490251 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.9e-175
Identity = 304/360 (84.44%), Postives = 339/360 (94.17%), Query Frame = 0


Query: 1   MDENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLS 60
           MD+ G+KESNKLKIEEE SD+KV+VWKYIFGFVEMAV+KCAIELKIGD IES+G SMTLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60

Query: 61  ELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLES 120
           +LSSSL+ SPLLLHR+LRFLVHRGIFKEEK+++SYS TPMSRLLTTTGPNS+APIHLLES
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLES 120

Query: 121 SPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVV 180
           SP M+APWH LSAC+KAN HEN SQPFE+AHGMDLW+YA+ANPSH+ L+NE MACFAR+ 
Sbjct: 121 SPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLF 180

Query: 181 ILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGV 240
           ILPAVL+RCGEIFDGVG LVDVGGGNGTCLSILVKACPWIKGINFDLPHVVS S+EYEGV
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGV 240

Query: 241 QHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENE 300
           QHVGG+MFDS+PKADAAFIMEVLHDWDDEECIKIL+N KEAIP+KTGKVIIVE+VIDE E
Sbjct: 241 QHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKE 300

Query: 301 -KISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP 360
            +IS+ SD++L MDMIMMAHTIKGKERTN EWAN+LQKAGFS+YTITPTT+IQSVIQAFP
Sbjct: 301 DQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

[Close]

BLAST of Bhi05G001426 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 303/360 (84.17%), Postives = 339/360 (94.17%), Query Frame = 0


Query: 1   MDENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLS 60
           MD+ G+KESNKLKIEEE SD+KV+VWKYIFGFVEMAV+KCAIELKIGD IES+G S+TLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLS 60

Query: 61  ELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLES 120
           +LSSSL+ SPLLLHR+LRFLVHRGIFKEEK+++SYS TPMSRLLTTTGPNS+APIHLLES
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLES 120

Query: 121 SPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVV 180
           SP M+APWH LSAC+KAN HEN SQPFE+AHGMDLW+YA+ANPSH+ L+NE MACFAR+ 
Sbjct: 121 SPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLF 180

Query: 181 ILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGV 240
           ILPAVL+RCGEIFDGVG LVDVGGGNGTCLSILVKACPWIKGINFDLPHVVS S+EYEGV
Sbjct: 181 ILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGV 240

Query: 241 QHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENE 300
           QHVGG+MFDS+PKADAAFIMEVLHDWDDEECIKIL+N KEAIP+KTGKVIIVE+VIDE E
Sbjct: 241 QHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKE 300

Query: 301 -KISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAFP 360
            +IS+ SD++L MDMIMMAHTIKGKERTN EWAN+LQKAGFS+YTITPTT+IQSVIQAFP
Sbjct: 301 DQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

[Close]

BLAST of Bhi05G001426 vs. ExPASy TrEMBL
Match: A0A6J1GLV0 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455162 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.6e-152
Identity = 279/359 (77.72%), Postives = 321/359 (89.42%), Query Frame = 0


Query: 1   MDENGSKESNKLKIEEEE-SDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTL 60
           MD  GSK+S KLKIEEEE +D+KV+VWKYIFGFVEMAVIKCAIELKIGDTIE++GG MTL
Sbjct: 1   MDNIGSKDS-KLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTL 60

Query: 61  SELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLE 120
           ++LSSSLN SPLLLHR+LRFL+HRGIFKEE  T+SY+QTPMSRLLT++G +SLAP+H+LE
Sbjct: 61  TDLSSSLNCSPLLLHRILRFLIHRGIFKEE-PTKSYAQTPMSRLLTSSGAHSLAPMHVLE 120

Query: 121 SSPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARV 180
           SS  M+APWH LSA LKAND    SQPFE+AHG DLW Y +AN SHNK++NE MA FARV
Sbjct: 121 SSHVMLAPWHNLSAYLKAND--GGSQPFEMAHGKDLWRYCAANSSHNKVMNEGMASFARV 180

Query: 181 VILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEG 240
           VI+PAVL+RCG +F+GVGSLVDVGGGNG+CLS+LVKA PWI+GINFDLPHVVS SQEYEG
Sbjct: 181 VIVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEG 240

Query: 241 VQHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDEN 300
           V+HVGGNMFD +PKADAAFIM  LHDWDDE+CIKILKN KEAIP+KTGKVIIVEVVIDE 
Sbjct: 241 VRHVGGNMFDFVPKADAAFIMNALHDWDDEDCIKILKNCKEAIPEKTGKVIIVEVVIDEK 300

Query: 301 EKISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAF 359
           ++ S +SDIRLM+DMIMMAHTIKGKERT  EWA VLQ+AGFS +TITPT++IQS+IQ F
Sbjct: 301 DE-SMVSDIRLMLDMIMMAHTIKGKERTREEWAYVLQQAGFSRHTITPTSTIQSIIQGF 354

[Close]

BLAST of Bhi05G001426 vs. ExPASy TrEMBL
Match: A0A6J1HWX9 ((RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468232 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.6e-147
Identity = 270/358 (75.42%), Postives = 313/358 (87.43%), Query Frame = 0


Query: 1   MDENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLS 60
           MD  GSK+S K K EEE +D+K++VWKYIFGFVEMAVIKCAIELKI DTI+++GG MTL 
Sbjct: 1   MDNIGSKDS-KFKREEEGNDIKIQVWKYIFGFVEMAVIKCAIELKIADTIDAHGGLMTLP 60

Query: 61  ELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLES 120
           ELSSSLN SPLLL R+LRFL+HRGIFKEE   +SY+QTPMSRLLT++G +SLAPIH+LES
Sbjct: 61  ELSSSLNCSPLLLRRILRFLIHRGIFKEE-PIKSYAQTPMSRLLTSSGAHSLAPIHVLES 120

Query: 121 SPAMVAPWHYLSACLKANDHENESQPFELAHGMDLWSYASANPSHNKLVNEAMACFARVV 180
           S  M+APWH LSA LKAN+    +QPFE+AHG DLWSY +AN SHN ++NE MA F+RVV
Sbjct: 121 SLVMLAPWHNLSAYLKANN--GGTQPFEMAHGKDLWSYCAANSSHNMVMNEGMASFSRVV 180

Query: 181 ILPAVLQRCGEIFDGVGSLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSGSQEYEGV 240
           I+PAVL+RCG +F+GVGSLVDVGGGNG+CLS+LVKA PWI+GINFDLPHVVS SQEYEGV
Sbjct: 181 IVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEGV 240

Query: 241 QHVGGNMFDSIPKADAAFIMEVLHDWDDEECIKILKNVKEAIPKKTGKVIIVEVVIDENE 300
           +HVGGNMFD +PKADAAFIM  LHDWDDEECIKILKN KEAIP+KTGKVIIVEVVIDE E
Sbjct: 241 RHVGGNMFDFVPKADAAFIMNALHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKE 300

Query: 301 KISQLSDIRLMMDMIMMAHTIKGKERTNVEWANVLQKAGFSHYTITPTTSIQSVIQAF 359
           + S +SD+RLM+DMIM+AHTIKGKERT  EWA VLQ AGFS +TITPT++IQS+IQ F
Sbjct: 301 E-SMVSDVRLMLDMIMIAHTIKGKERTREEWAYVLQGAGFSRHTITPTSTIQSIIQGF 353

The following BLAST results are available for this feature:

BLAST of Bhi05G001426 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	3.3e-94	48.52	O-methyltransferase family protein	[more]
AT4G35150.1	9.7e-78	43.96	O-methyltransferase family protein	[more]
AT5G54160.1	2.2e-45	33.65	O-methyltransferase 1	[more]
AT1G77530.1	1.7e-42	35.33	O-methyltransferase family protein	[more]
AT1G77520.1	2.9e-42	35.28	O-methyltransferase family protein	[more]

BLAST of Bhi05G001426 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	4.6e-93	48.52	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
Q6WUC2	1.7e-71	42.74	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]
Q6WUC1	6.1e-69	43.18	(RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT P...	[more]
Q9LEL6	3.0e-68	40.79	(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1	[more]
B0ZB56	5.2e-68	43.21	Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1	[more]

BLAST of Bhi05G001426 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K0Q2	2.2e-178	85.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1	[more]
A0A1S3BHY1	3.9e-175	84.44	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5D3DI94	1.1e-174	84.17	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1GLV0	4.6e-152	77.72	xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HWX9	2.6e-147	75.42	(RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 37..81 e-value: 7.8E-10 score: 38.6
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 106..359 e-value: 8.0E-78 score: 263.1
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 36..357 e-value: 2.4E-87 score: 290.5
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 10..357
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 199..296 e-value: 0.0044306 score: 34.7131
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 3..359 e-value: 7.4E-118 score: 391.5
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 10..357
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 26..359 score: 77.176323
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 128..341 e-value: 4.5E-53 score: 179.7
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 7..104 e-value: 2.9E-25 score: 89.9
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 14..107
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 110..355

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M001426	Bhi05M001426	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity