Bhi05G001223 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G001223
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	O-methyltransferase family protein
Location	chr5: 48537135 .. 48537431 (+)
RNA-Seq Expression	Bhi05G001223
Synteny	Bhi05G001223

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACATGGTTGGAGTGATGAAGAATGTGTGAAGATTCTGAAGAATTGCAAAACAGCCATAACGAGCAATGGTCACAAAGGAAAAGTGATTGTAATTGATATGGTGGTCGACAATAAAAGAGATGAGAACTCGATCAAAACTCAACTTTTCTTCGACATGTTGATGATGATTCTTGCTGGTGGAAAAGAGAGAGAAGAAGAGGAGTGGGCTCAATTGATCAAAGAAGCTGGATTTGGTGGCTACAACATTATCCCAATTATGGGCTTAAGGTCTATTATAGAGATTTACCCATAA

mRNA sequence

ATGTTACATGGTTGGAGTGATGAAGAATGTGTGAAGATTCTGAAGAATTGCAAAACAGCCATAACGAGCAATGGTCACAAAGGAAAAGTGATTGTAATTGATATGGTGGTCGACAATAAAAGAGATGAGAACTCGATCAAAACTCAACTTTTCTTCGACATGTTGATGATGATTCTTGCTGGTGGAAAAGAGAGAGAAGAAGAGGAGTGGGCTCAATTGATCAAAGAAGCTGGATTTGGTGGCTACAACATTATCCCAATTATGGGCTTAAGGTCTATTATAGAGATTTACCCATAA

Coding sequence (CDS)

ATGTTACATGGTTGGAGTGATGAAGAATGTGTGAAGATTCTGAAGAATTGCAAAACAGCCATAACGAGCAATGGTCACAAAGGAAAAGTGATTGTAATTGATATGGTGGTCGACAATAAAAGAGATGAGAACTCGATCAAAACTCAACTTTTCTTCGACATGTTGATGATGATTCTTGCTGGTGGAAAAGAGAGAGAAGAAGAGGAGTGGGCTCAATTGATCAAAGAAGCTGGATTTGGTGGCTACAACATTATCCCAATTATGGGCTTAAGGTCTATTATAGAGATTTACCCATAA

Protein sequence

MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSIKTQLFFDMLMMILAGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP

Homology

[Close]

BLAST of Bhi05G001223 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 78.6 bits (192), Expect = 3.2e-15
Identity = 44/106 (41.51%), Postives = 65/106 (61.32%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVV-DNK-------RDENSIKTQLFF 60
           +LH W D++C+KILKNCK A+  N   GKV++++ V+ +NK       RDE     +L  
Sbjct: 277 VLHDWGDKDCIKILKNCKEAVPPN--IGKVLIVESVIGENKKTMIVDERDEKLEHVRLML 336

Query: 61  DMLMMI-LAGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIY 98
           DM+MM   + GKER  +EW  ++KEAGF  Y +  I  ++S+I  Y
Sbjct: 337 DMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLIIAY 380

[Close]

BLAST of Bhi05G001223 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 77.8 bits (190), Expect = 5.4e-15
Identity = 46/101 (45.54%), Postives = 63/101 (62.38%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSI--KTQLFFDMLMMI 60
           +LH W+DE+CV+ILKNCK A+      G++IVI+M+V  +  E  +  K  L  D+ MM 
Sbjct: 265 ILHDWNDEKCVEILKNCKKALPET---GRIIVIEMIVPREVSETDLATKNSLSADLTMMS 324

Query: 61  L-AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
           L +GGKER ++E+  L KEAGF    II       IIE+YP
Sbjct: 325 LTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWIIELYP 362

[Close]

BLAST of Bhi05G001223 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 77.8 bits (190), Expect = 5.4e-15
Identity = 46/101 (45.54%), Postives = 63/101 (62.38%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSI--KTQLFFDMLMMI 60
           +LH W+DE+CV+ILKNCK A+      G++IVI+M+V  +  E  +  K  L  D+ MM 
Sbjct: 265 ILHDWNDEKCVEILKNCKKALPET---GRIIVIEMIVPREVSETDLATKNSLSADLTMMS 324

Query: 61  L-AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
           L +GGKER ++E+  L KEAGF    II       IIE+YP
Sbjct: 325 LTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWIIELYP 362

[Close]

BLAST of Bhi05G001223 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 4.6e-14
Identity = 40/106 (37.74%), Postives = 62/106 (58.49%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNK--------RDENSIKTQLFF 60
           +LH W D++C+KILKNCK A+  N   GKV++++ V+  K        RD+     +L  
Sbjct: 220 VLHDWGDKDCIKILKNCKEAVLPN--IGKVLIVECVIGEKKNTMIAEERDDKLEHVRLQL 279

Query: 61  DMLMMI-LAGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIY 98
           DM+MM+  + GKER  +EW  ++ EAGF  Y +     ++S+I  Y
Sbjct: 280 DMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

[Close]

BLAST of Bhi05G001223 vs. TAIR 10
Match: AT1G76790.1 (O-methyltransferase family protein )

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-12
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSIKTQLFFDM-LMMI- 60
           +LH W+DE+C KILKNC  A+  N   GKVIV+++V  ++ D   + + + FDM L+M+ 
Sbjct: 268 ILHDWTDEDCEKILKNCWKALPEN---GKVIVMEVVTPDEADNRDVISNIAFDMDLLMLT 327

Query: 61  -LAGGKEREEEEWAQLIKEAGFGGYNII 86
            L+GGKER   E+  +   +GF   N +
Sbjct: 328 QLSGGKERSRAEYVAMAANSGFPRCNFV 352

[Close]

BLAST of Bhi05G001223 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 7.6e-30
Identity = 64/101 (63.37%), Postives = 80/101 (79.21%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAIT-SNGHKGKVIVIDMVVDNKRDEN--SIKTQLFFDMLMM 60
           +LH W+DEE VKILK CK AI+ SN   GKVI+IDM V+N++DE+  S +TQLFFDMLMM
Sbjct: 277 ILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENEKDEDDESYETQLFFDMLMM 336

Query: 61  ILAGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
            L  G+ER E+EWA+L K+AGF  Y I PI+GLRS+IE+YP
Sbjct: 337 ALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

[Close]

BLAST of Bhi05G001223 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-28
Identity = 59/99 (59.60%), Postives = 75/99 (75.76%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMV-VDNKRDENSIKTQLFFDMLMMIL 60
           +LH WS+EECVKILK C+ AI S  + GKVI+IDM+ + N+ D  S +TQLFFDM MMI 
Sbjct: 259 ILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYKSTETQLFFDMTMMIF 318

Query: 61  AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
           A G+ER+E EW +L  +AGF  Y I PI+GLRS+IE+YP
Sbjct: 319 APGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

[Close]

BLAST of Bhi05G001223 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.6e-24
Identity = 54/100 (54.00%), Postives = 70/100 (70.00%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVD--NKRDENSIKTQLFFDMLMMI 60
           +LH W+DEEC+KILK CK AI S    GK+I+IDMVV+  NK    S +TQLF+D+ +M 
Sbjct: 273 ILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVNDHNKGSYESTETQLFYDLTLMA 332

Query: 61  LAGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
           L  G ER E EW +L   AGF  Y I P++GL+SIIE++P
Sbjct: 333 LLTGTERTETEWKKLFVAAGFTSYIISPVLGLKSIIEVFP 372

[Close]

BLAST of Bhi05G001223 vs. ExPASy Swiss-Prot
Match: O22308 (Isoflavone-7-O-methyltransferase 6 OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.8e-24
Identity = 52/99 (52.53%), Postives = 72/99 (72.73%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSI-KTQLFFDMLMMIL 60
           +LH W+D++C++ILK CK A+T++G +GKV +IDMV+D K+DEN + + +L  D+ M  L
Sbjct: 255 ILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMACL 314

Query: 61  AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
             GKER EEEW +L  EAGF  Y I P+ G  S+IEIYP
Sbjct: 315 -NGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 352

[Close]

BLAST of Bhi05G001223 vs. ExPASy Swiss-Prot
Match: O24529 (Isoflavone-7-O-methyltransferase 8 OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.8e-24
Identity = 52/99 (52.53%), Postives = 72/99 (72.73%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSI-KTQLFFDMLMMIL 60
           +LH W+D++C++ILK CK A+T++G +GKV +IDMV+D K+DEN + + +L  D+ M  L
Sbjct: 255 ILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDKKKDENQVTQIKLLMDVNMACL 314

Query: 61  AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
             GKER EEEW +L  EAGF  Y I P+ G  S+IEIYP
Sbjct: 315 -NGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 352

[Close]

BLAST of Bhi05G001223 vs. ExPASy TrEMBL
Match: A0A6J1C1F3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.3e-36
Identity = 75/98 (76.53%), Postives = 87/98 (88.78%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSIKTQLFFDMLMMILA 60
           +LH W+DEECV+ILK CK AIT+NG KGK+IVIDMVV NK+ ++SI+TQLFFDMLMM LA
Sbjct: 265 ILHDWNDEECVEILKKCKEAITNNGKKGKMIVIDMVVGNKQGDDSIETQLFFDMLMMNLA 324

Query: 61  GGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
           GGKER+E+EWAQLIKEAG  GY I PIMGLRS+IEIYP
Sbjct: 325 GGKERDEKEWAQLIKEAGLSGYKIFPIMGLRSLIEIYP 362

[Close]

BLAST of Bhi05G001223 vs. ExPASy TrEMBL
Match: A0A6J1GK49 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455045 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-35
Identity = 74/99 (74.75%), Postives = 90/99 (90.91%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNK-RDENSIKTQLFFDMLMMIL 60
           +LH WSD+ECVKILKNCK AITSNG+KGKV+VIDMVV  K ++ +S +TQLF++MLMMIL
Sbjct: 270 ILHDWSDQECVKILKNCKAAITSNGYKGKVMVIDMVVGYKGKESDSYETQLFYEMLMMIL 329

Query: 61  AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
            GGKEREE+EWA+LI+EAGF GYNI+PI+GLRS+IEIYP
Sbjct: 330 VGGKEREEKEWAKLIEEAGFSGYNILPIVGLRSLIEIYP 368

[Close]

BLAST of Bhi05G001223 vs. ExPASy TrEMBL
Match: A0A6J1HZ45 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468224 PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.8e-35
Identity = 73/99 (73.74%), Postives = 89/99 (89.90%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNK-RDENSIKTQLFFDMLMMIL 60
           +LH WSD ECV+ILKNCK AITS+G+KGKV+VIDMVV  K ++ +S +TQLF+DMLMMIL
Sbjct: 270 ILHDWSDPECVRILKNCKAAITSHGYKGKVMVIDMVVGYKGKESDSYETQLFYDMLMMIL 329

Query: 61  AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
            GGKEREE+EWA+LI+EAGF GYNI+PI+GLRS+IEIYP
Sbjct: 330 VGGKEREEKEWAKLIREAGFSGYNILPILGLRSLIEIYP 368

[Close]

BLAST of Bhi05G001223 vs. ExPASy TrEMBL
Match: A0A5D3DD88 (Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00380 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 8.1e-35
Identity = 73/99 (73.74%), Postives = 88/99 (88.89%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKR-DENSIKTQLFFDMLMMIL 60
           +LH WSDEECVKILK CK AITSNG+KGKV+VID+V+ NK+ D++SI+TQLF DMLMM++
Sbjct: 258 ILHDWSDEECVKILKKCKEAITSNGNKGKVMVIDLVLFNKKNDQDSIETQLFHDMLMMVV 317

Query: 61  AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
            GGKEREE+EWA+LIKEAGF  Y I PI+GLRS+IEIYP
Sbjct: 318 TGGKEREEKEWAKLIKEAGFSAYKIFPILGLRSLIEIYP 356

[Close]

BLAST of Bhi05G001223 vs. ExPASy TrEMBL
Match: A0A5A7UWR4 (Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G00130 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 8.1e-35
Identity = 73/99 (73.74%), Postives = 87/99 (87.88%), Query Frame = 0


Query: 1   MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDN-KRDENSIKTQLFFDMLMMIL 60
           +LH WSDEECVKILKNCK AITSNG KGKV+VID+V+ N K D++SI++QLFFDMLMM+ 
Sbjct: 228 ILHDWSDEECVKILKNCKEAITSNGKKGKVMVIDLVLFNTKNDQDSIESQLFFDMLMMVE 287

Query: 61  AGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP 99
            GGKEREE+EWA+L+KEAGF  Y I PI+GLRS+IEIYP
Sbjct: 288 VGGKEREEKEWAKLVKEAGFSAYKIFPILGLRSLIEIYP 326

The following BLAST results are available for this feature:

BLAST of Bhi05G001223 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	3.2e-15	41.51	O-methyltransferase family protein	[more]
AT1G51990.1	5.4e-15	45.54	O-methyltransferase family protein	[more]
AT1G51990.2	5.4e-15	45.54	O-methyltransferase family protein	[more]
AT4G35150.1	4.6e-14	37.74	O-methyltransferase family protein	[more]
AT1G76790.1	1.5e-12	40.91	O-methyltransferase family protein	[more]

BLAST of Bhi05G001223 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	7.6e-30	63.37	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	3.2e-28	59.60	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
A0A0N9HMN6	1.6e-24	54.00	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]
O22308	2.8e-24	52.53	Isoflavone-7-O-methyltransferase 6 OS=Medicago sativa OX=3879 PE=2 SV=1	[more]
O24529	2.8e-24	52.53	Isoflavone-7-O-methyltransferase 8 OS=Medicago sativa OX=3879 PE=1 SV=1	[more]

BLAST of Bhi05G001223 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1F3	3.3e-36	76.53	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE...	[more]
A0A6J1GK49	1.3e-35	74.75	trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1HZ45	4.8e-35	73.74	trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A5D3DD88	8.1e-35	73.74	Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5A7UWR4	8.1e-35	73.74	Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..98 e-value: 5.6E-32 score: 112.9
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 1..98
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 2..79 e-value: 8.1E-21 score: 74.3
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 1..98
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 1..98 score: 31.464968
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 1..96

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M001223	Bhi05M001223	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
cellular_component	GO:0048046	apoplast
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity