Bhi05G001095 (gene) Wax gourd (B227) v1

Overview
NameBhi05G001095
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
Locationchr5: 43987474 .. 43991847 (+)
RNA-Seq ExpressionBhi05G001095
SyntenyBhi05G001095
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACCGGAAATCGGAACTGTTTCAGAGACTCCTGCGATCACAAACCTCAGTAGTTCGTGCATAGAGAAGAAGCATTTTGGAATCCCCACTGTGAGTAGCGGTGAAGAACATTGGCTTACACCATTTCCCATGGCTGCGATCTTAGCTCGGAAATCCCTTCTCGCTCTTCGTGCTCGTCAGCTTGTAAGCAATCTCCTCTTCTTTGCCCTATTTTTTCTACTCTTCCCCTCTCACCCTTTTCCCGGCGATGGACTTGTAAGAGCCCATTGCTTCAATATGGACGATTCTGAGATTTTCTTCTGTTTTGGGATTTCTAGTGTTTGTAATTCAATCGAGGTTTTCTTGGTGCTGTTTTTTCTTATTAGTTTTTGATATTCGAGTCGTAGAACAGATGGGGTCTTCGTTTCCCCTTTTTTTTTTTTTTTTTTTTTGAATCATACAATTATGGTTATTGGGAATTGTTGTGTGTAATTTATTTTTTCCGTTGGGTCAACATTTTTTATATGGAAATGGTATTGCGTGTCCTTTCTTTTCCCTAACTTGGAAGGATTTGATGTTTTTAATCGTCCCATTTTGAGGGAGATTATGTTAGAACGAGGAACTTGAAGTACTAAATGCTTGCAGACTAGGTAGTTGATGGTTAGGGGGTTTATACAATGCAATATCCTGAACTTTTCTGGAACAACGTAGGCTGTAATCTAGATATGTCGTAAAGATCTGTAGATGGTTTTCGGAATTTTGGGGGAGATGTTCAAGACTGTGAATTATGCGTTGTATGTTTTTTCCTTGAATTATATCAATGTGTTCACGGTTGGGGGAGAAAAAGAGAGATTAGTAGTATGGTGTTGTGCGTCTTATTTTTACTGAATAACCAATTACTTAGTCTGTAATCTTTATTATTTATCTATTTCTACGTTGGGGTGGGAGGGTTCTGTTCAATGTTCACCTTTGAAGGCCTTTTAGTGTTATTTTCTTTGATCTGAATATTTCACTTTGACATTTGATGATAAATATTATCTCCTAAGATTTAGTTATCATGGAGGTATTTACAATATTGATTGAAAACTTGATGGGAATTATGCCATAGATATCGTATTCCTCGATTCTGATTGTTGTAACAACTTTTTGTTACCCTTTACTTCATTTATCATGTGTATAATCTACTGTTGTTAGGCCGTCTCAGGACAAGCACTGCAAGGTTCTCCTTTTCATGGTCTCCGGTCAAGTGCACACTTGTACAGTACCAAAAAAGGTTTTGTTTCTAGTTATCATCATCTTAACAAAGATTTTGTATTACCATATTGGCATAGCAGCATAATTAACGGTTGAAGCTGAACTAAGCAGTTTGTAGTTGATCTGCGAGACACATACTTATGCGTTTTATTTCTGCCAGAATGGTGCTATAGATTTTTTTTGTTTGCTTAAAATTATTTTCCATATCATTACTCCTGCAGAAGATGAAGAAAGAGAGCAGCTTGCCAAGGAGATCTCGAAGGATTGGAGTTCTAGTATTTTTTTATTTCCTTCCATCGTTTCTTCACTATTTCCCTTTAAATATTTTTCGGTTGTTTGTTCTATTATATTTCGTTCTTATGAAATTAATTTCCTGGCTATTTTTAGTTTTTGAGCGTAGCATAAACACACTATTTCTCACTGAAATGGTCCGGGGTCTGATGCTGACTCTTAAATACTTCTTTGAGAAAAAAGTCACTGTAAGTGTCTTCCAATCAACTTTCTTTGGCTCTAAGCTAAAATTCTTGGTGATCCTGATTTCTGATTATTGTAAATGTGTCATGCAGATCAATTATCCATTTGAGAAAGGCCCTCTAAGTCCTCGTTTTCGGGGAGAGCATGCACTCCGACGATATCCAACTGGAGAAGAACGATGCATTGCATGCAAACTTTGTGAAGCTGTAAGAATCTGAATCTATATTTAACTTTTTGTTTGAACATGTAGTTCTACAACTTTATGTTAGAGAAATCTTTATATTTCTTGCCATCATTGATTTTAGGTTTAATTACAAGTTTAGTCCCTAAGGGGTTGTTTGAGGCGCTGAGTTGGTTATTGTAGTTAGTGGGTTATAATAGTTTGTAGGTTATAATAGTTTGCACATAATAGTCTGTGTTTGGGGTGTAGACTATTTTAATCTGGATAGGAAATGGTAAACATTGTAAGAGAGAGAGGATGAGTAGGAAATAGTAAACAAGGTAACAAAGAGGGGTTTGAAATAGTAATACTGTAGTTAAATTGTAGGTTATATAGTTGGAAACACTAATTATTATAATTGGGCCCCCAAACATGGTGTTAGCTATAATAGCTCACTCCATCCACTTGAAGTTGGGACCCAAACACCTTTAAGGTTTGTATCTATTGGATCATTGAACTTCTTTCAATTTATCCCTTGACTTTAAAAAGTGCCTAATAGATTTTTAAAATTTCGACCATTAAGTTTTTGAACTTCAAAAAGTATCTAATGAATTCTTAAGCTTTTAATTTTGTGTCCAATAGGTCCTTGACCTATTCGACATATTTTTTTAATTTCACGTATCTAGTATCTACTTAACATAAAATGAAATTCTAGGATTCCATCAGAGATAAAATTCAAGTTTATATCTAATTGATCAATTATCAATAATTTTTTTTATATGAAGGTCCTATTAGATACAAAATTGATGTTCAAGGACTTGTTAGCGCACTATAGCTTGGTTGATATTTTACTGTCCCTTGATGTTTTGAAGTGCATATTGTAATATTTTGTCGAAGTTGAAATCTTACTAGGATAGAATGTATCTAAGGTGATCGTGCATGGCTTTAATCCTTGACTGAAGAGTGTATTTGAGAGGGGTTCTCTAAGAGATGCAACTTTGACAGCTATTTGTGCTTATATTTCTCATCATTGGTTTATTAAGATCGACTGCATCTGCATGGTAGTTTTGCTTTGTAACCTCGTAACACAGTGGATAATAACATATTCACATTTTAAATTTTATGCAGAACTTTGATAATGTTACATAAACCATAATAGTTTAGCAGGCCATGCTTAAAATATTTATGGTTTTGATCTCGTGATAAATTAAATACTTCGAAATGATACATTTGAGTGTACACTTATCTGATCATTACTTTGAGTTGTGGCTTTGAACTTATGTCTGTTATTCTATTTTGATTTTCTTGTTAGATTTGTCCTGCTCAAGCAATCACAATCGAAGCTGAGGAACGGGAAGATGGTAGCCGTAGAACCACAAGGTGATCACACCTCCAAGCCATCTCTTTATTGTTATTTCTGTGTCCCTAACTAGTTTTAATTTTTAGGTATGACATTGACATGACCAAATGCATCTACTGTGGGTTCTGTCAAGAGGCATGCCCTGTTGACGCGATTGTTGAAGGACCCAACTTTGAGTTTGCTACAGAGACTCACGAGGTTGATCTTATTATGTTTTGAATATTATAATAAGCTTCTAACCTTGCTGAAGTATGTTAGCCTCATAGTTCTGTGATGTTGTAGGAGCTTCTCTATGACAAAGAAAAGTTGCTCGAGAATGGAGACCGCTGGGAGACCGAGATCGCAGAAAACCTCAGATCTGAAAGCCTCTATCGCTGATACATTTTCCCATGGTGCACTCTGGATATGACTTTTCTTAGTGCGTTCATAATAAGATAAACCTGAGCATGGTGTTGAAATTTTTTTTGTATTCTTGGTGTGCATGAGTCGAGAACATAATTCTTGCTGCTTACTACGGCCCAAGTTTGGGATTTTTCTCGGTATCTTTTGTTTTTCTTCCCAATAAAAATGGGAATGTATGGTTTCTCATGCGGTTGTAATGTAGGTAAAAATATGAACAATACCATCGATATATCTTCAATGTTCTTCCCGACTTCATGCTCTGGTATTGAGTTTGGAAAGAGAAAAAAAAAAAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGACCAGAAAATTTCATAGAATGCCCATTTGAATTCCCTTTTATTGAGTTCGAGTATTCCAATATACATTTGAATTTAAGATAAATGAAGATGGATATTGTTAGAATTAAGATAGAGTAACACTTGAAAGGTACTAAATTAGAAGAGATGTCTATTTTCTCTAGGGAAGTGGTGCTTTGTGGAGACATGTCCCGAATAGGGTAGAGAATTCTTGTTTAGGTGAAGGGGGTAGGAGAAATTTTTATTTTTTGCAAGCTAAAAGGGGATGGGGAATCTCTGTCTTGATTCTCGTGAAATTAAATGGAAAGGTTTATGAGAGTGGAGAATGAAATAAGAGGTGAGGATGGGGTCGGAACCCATCCCTGCCTCTAGGGTTGTTTATAGTACAATATTTCACTTTTGCATTCTTTATGAATATTTA

mRNA sequence

CGACCGGAAATCGGAACTGTTTCAGAGACTCCTGCGATCACAAACCTCAGTAGTTCGTGCATAGAGAAGAAGCATTTTGGAATCCCCACTGTGAGTAGCGGTGAAGAACATTGGCTTACACCATTTCCCATGGCTGCGATCTTAGCTCGGAAATCCCTTCTCGCTCTTCGTGCTCGTCAGCTTGCCGTCTCAGGACAAGCACTGCAAGGTTCTCCTTTTCATGGTCTCCGGTCAAGTGCACACTTGTACAGTACCAAAAAAGAAGATGAAGAAAGAGAGCAGCTTGCCAAGGAGATCTCGAAGGATTGGAGTTCTATTTTTGAGCGTAGCATAAACACACTATTTCTCACTGAAATGGTCCGGGGTCTGATGCTGACTCTTAAATACTTCTTTGAGAAAAAAGTCACTATCAATTATCCATTTGAGAAAGGCCCTCTAAGTCCTCGTTTTCGGGGAGAGCATGCACTCCGACGATATCCAACTGGAGAAGAACGATGCATTGCATGCAAACTTTGTGAAGCTATTTGTCCTGCTCAAGCAATCACAATCGAAGCTGAGGAACGGGAAGATGGTAGCCGTAGAACCACAAGGTATGACATTGACATGACCAAATGCATCTACTGTGGGTTCTGTCAAGAGGCATGCCCTGTTGACGCGATTGTTGAAGGACCCAACTTTGAGTTTGCTACAGAGACTCACGAGGAGCTTCTCTATGACAAAGAAAAGTTGCTCGAGAATGGAGACCGCTGGGAGACCGAGATCGCAGAAAACCTCAGATCTGAAAGCCTCTATCGCTGATACATTTTCCCATGGTGCACTCTGGATATGACTTTTCTTAGTGCGTTCATAATAAGATAAACCTGAGCATGGTGTTGAAATTTTTTTTGTATTCTTGGTGTGCATGAGTCGAGAACATAATTCTTGCTGCTTACTACGGCCCAAGTTTGGGATTTTTCTCGGTATCTTTTGTTTTTCTTCCCAATAAAAATGGGAATGTATGGTTTCTCATGCGGTTGTAATGTAGGTAAAAATATGAACAATACCATCGATATATCTTCAATGTTCTTCCCGACTTCATGCTCTGGTATTGAGTTTGGAAAGAGAAAAAAAAAAAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGACCAGAAAATTTCATAGAATGCCCATTTGAATTCCCTTTTATTGAGTTCGAGTATTCCAATATACATTTGAATTTAAGATAAATGAAGATGGATATTGTTAGAATTAAGATAGAGTAACACTTGAAAGGTACTAAATTAGAAGAGATGTCTATTTTCTCTAGGGAAGTGGTGCTTTGTGGAGACATGTCCCGAATAGGGTAGAGAATTCTTGTTTAGGTGAAGGGGGTAGGAGAAATTTTTATTTTTTGCAAGCTAAAAGGGGATGGGGAATCTCTGTCTTGATTCTCGTGAAATTAAATGGAAAGGTTTATGAGAGTGGAGAATGAAATAAGAGGTGAGGATGGGGTCGGAACCCATCCCTGCCTCTAGGGTTGTTTATAGTACAATATTTCACTTTTGCATTCTTTATGAATATTTA

Coding sequence (CDS)

ATGGCTGCGATCTTAGCTCGGAAATCCCTTCTCGCTCTTCGTGCTCGTCAGCTTGCCGTCTCAGGACAAGCACTGCAAGGTTCTCCTTTTCATGGTCTCCGGTCAAGTGCACACTTGTACAGTACCAAAAAAGAAGATGAAGAAAGAGAGCAGCTTGCCAAGGAGATCTCGAAGGATTGGAGTTCTATTTTTGAGCGTAGCATAAACACACTATTTCTCACTGAAATGGTCCGGGGTCTGATGCTGACTCTTAAATACTTCTTTGAGAAAAAAGTCACTATCAATTATCCATTTGAGAAAGGCCCTCTAAGTCCTCGTTTTCGGGGAGAGCATGCACTCCGACGATATCCAACTGGAGAAGAACGATGCATTGCATGCAAACTTTGTGAAGCTATTTGTCCTGCTCAAGCAATCACAATCGAAGCTGAGGAACGGGAAGATGGTAGCCGTAGAACCACAAGGTATGACATTGACATGACCAAATGCATCTACTGTGGGTTCTGTCAAGAGGCATGCCCTGTTGACGCGATTGTTGAAGGACCCAACTTTGAGTTTGCTACAGAGACTCACGAGGAGCTTCTCTATGACAAAGAAAAGTTGCTCGAGAATGGAGACCGCTGGGAGACCGAGATCGCAGAAAACCTCAGATCTGAAAGCCTCTATCGCTGA

Protein sequence

MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Homology
BLAST of Bhi05G001095 vs. TAIR 10
Match: AT1G16700.1 (Alpha-helical ferredoxin )

HSP 1 Score: 394.4 bits (1012), Expect = 5.9e-110
Identity = 195/222 (87.84%), Postives = 206/222 (92.79%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MA++LAR+S  ALRAR LA SGQ LQGS   GL+S A  Y + K+DEE EQLAKEISKDW
Sbjct: 1   MASLLARRSFSALRARHLAFSGQGLQGSHLCGLQSRAISYGSNKDDEEAEQLAKEISKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           S++FERS+NTLFLTEMVRGL LTLKYFF+ KVTINYPFEKGPLSPRFRGEHALRRYPTGE
Sbjct: 61  STVFERSMNTLFLTEMVRGLSLTLKYFFDPKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. TAIR 10
Match: AT1G79010.1 (Alpha-helical ferredoxin )

HSP 1 Score: 390.6 bits (1002), Expect = 8.5e-109
Identity = 193/222 (86.94%), Postives = 205/222 (92.34%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MA+ILAR+SL  LRAR L +SGQALQGS    L+S    Y + K+DEE EQL+KEISKDW
Sbjct: 1   MASILARRSLNTLRARHLVLSGQALQGSHLSRLQSRGISYGSNKDDEEAEQLSKEISKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           +++FERSINTLFLTEMVRGL LTLKYFF+ KVTINYPFEKGPLSPRFRGEHALRRYPTGE
Sbjct: 61  NTVFERSINTLFLTEMVRGLSLTLKYFFDPKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. TAIR 10
Match: ATCG01090.1 (NADPH dehydrogenases )

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-15
Identity = 43/121 (35.54%), Postives = 63/121 (52.07%), Query Frame = 0

Query: 78  RGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQA 137
           +G M+TL +     VTI YP+EK   S RFRG     R     ++CIAC++C  +CP   
Sbjct: 24  QGFMITLSHTNRLPVTIQYPYEKLITSERFRG-----RIHFEFDKCIACEVCVRVCPIDL 83

Query: 138 ITIEAE-EREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEELLYD 197
             ++ + E     +R   Y ID   CI+CG C E CP + +     +EF+T    EL Y+
Sbjct: 84  PVVDWKLETNIRKKRLLNYSIDFGICIFCGNCVEYCPTNCLSMTEEYEFSTYDRHELNYN 139

BLAST of Bhi05G001095 vs. ExPASy Swiss-Prot
Match: Q9FX83 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g16700 PE=1 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 8.3e-109
Identity = 195/222 (87.84%), Postives = 206/222 (92.79%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MA++LAR+S  ALRAR LA SGQ LQGS   GL+S A  Y + K+DEE EQLAKEISKDW
Sbjct: 1   MASLLARRSFSALRARHLAFSGQGLQGSHLCGLQSRAISYGSNKDDEEAEQLAKEISKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           S++FERS+NTLFLTEMVRGL LTLKYFF+ KVTINYPFEKGPLSPRFRGEHALRRYPTGE
Sbjct: 61  STVFERSMNTLFLTEMVRGLSLTLKYFFDPKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. ExPASy Swiss-Prot
Match: P80269 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 392.9 bits (1008), Expect = 2.4e-108
Identity = 199/229 (86.90%), Postives = 211/229 (92.14%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFH-----GLRSSA--HLYSTKKEDEEREQLA 60
           MAAILARKSL ALR+RQL ++G  ++G+  +     G RS A  H +ST K+DEEREQLA
Sbjct: 1   MAAILARKSLSALRSRQLVLAGHTIEGTNGYNRTLLGTRSFATKHSFSTDKDDEEREQLA 60

Query: 61  KEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHAL 120
           KE+SKDW+S+FERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHAL
Sbjct: 61  KELSKDWNSVFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHAL 120

Query: 121 RRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACP 180
           RRY TGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACP
Sbjct: 121 RRYATGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACP 180

Query: 181 VDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           VDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 VDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 229

BLAST of Bhi05G001095 vs. ExPASy Swiss-Prot
Match: Q42599 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g79010 PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.2e-107
Identity = 193/222 (86.94%), Postives = 205/222 (92.34%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MA+ILAR+SL  LRAR L +SGQALQGS    L+S    Y + K+DEE EQL+KEISKDW
Sbjct: 1   MASILARRSLNTLRARHLVLSGQALQGSHLSRLQSRGISYGSNKDDEEAEQLSKEISKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           +++FERSINTLFLTEMVRGL LTLKYFF+ KVTINYPFEKGPLSPRFRGEHALRRYPTGE
Sbjct: 61  NTVFERSINTLFLTEMVRGLSLTLKYFFDPKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEA+CPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAVCPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. ExPASy Swiss-Prot
Match: O24143 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.2e-104
Identity = 195/230 (84.78%), Postives = 205/230 (89.13%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQ------GSPFHGLRSSA--HLYSTKKEDEEREQL 60
           MAAILARKSL ALR+RQL ++GQA Q           G R+ A    +ST K+DEEREQL
Sbjct: 1   MAAILARKSLSALRSRQLVLAGQAWQQGANTSNGTLLGTRTFATNDSFSTDKDDEEREQL 60

Query: 61  AKEISKDWSSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHA 120
           AKE+SKDW+S+FE+ INTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGE  
Sbjct: 61  AKELSKDWNSVFEQQINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEQP 120

Query: 121 LRRYPTGEERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEAC 180
           LRRYPTGEERCIACKLCEAICPAQAITIEAE REDGSRRTTRYDIDMTKCIYCGFCQEAC
Sbjct: 121 LRRYPTGEERCIACKLCEAICPAQAITIEAEAREDGSRRTTRYDIDMTKCIYCGFCQEAC 180

Query: 181 PVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PVDAIVEGPNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 230

BLAST of Bhi05G001095 vs. ExPASy Swiss-Prot
Match: O21233 (NADH-ubiquinone oxidoreductase subunit 8 OS=Reclinomonas americana OX=48483 GN=NAD8 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 5.3e-79
Identity = 138/161 (85.71%), Postives = 149/161 (92.55%), Query Frame = 0

Query: 62  SIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGEE 121
           +I  ++  TLFLTE+V+G+ LTL YFF KKVT+NYPFEKGPLSPRFRGEHALRRY TGEE
Sbjct: 2   TIINKTAQTLFLTELVKGMSLTLDYFFRKKVTLNYPFEKGPLSPRFRGEHALRRYQTGEE 61

Query: 122 RCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGP 181
           RCIACKLCEAICPAQAITIE+E R DGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGP
Sbjct: 62  RCIACKLCEAICPAQAITIESEPRIDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGP 121

Query: 182 NFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           NFEFATETHEELLYDKEKLL+NGDRWETEIA NL +E+LYR
Sbjct: 122 NFEFATETHEELLYDKEKLLQNGDRWETEIAANLANEALYR 162

BLAST of Bhi05G001095 vs. ExPASy TrEMBL
Match: A0A6J1C2E8 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111007833 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 7.1e-119
Identity = 218/222 (98.20%), Postives = 220/222 (99.10%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MAAILARKSLLALRAR LAVSGQALQGSPFHGLRSSAHL+STKK+DEEREQLAKEISKDW
Sbjct: 1   MAAILARKSLLALRARHLAVSGQALQGSPFHGLRSSAHLFSTKKDDEEREQLAKEISKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRY TGE
Sbjct: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYATGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. ExPASy TrEMBL
Match: A0A6J1I8H7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470147 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 216/222 (97.30%), Postives = 220/222 (99.10%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MAAILARKSLLALRARQ A+SGQALQGSPFHGLRSSAHLYSTK+EDEEREQLAKE+SKDW
Sbjct: 1   MAAILARKSLLALRARQFAISGQALQGSPFHGLRSSAHLYSTKREDEEREQLAKEMSKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           +SIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRY TGE
Sbjct: 61  NSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYATGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. ExPASy TrEMBL
Match: A0A6J1F0I7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111441225 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 216/222 (97.30%), Postives = 220/222 (99.10%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MAAILARKSLLALRARQ A+SGQALQGSPFHGLRSSAHLYSTK+EDEEREQLAKE+SKDW
Sbjct: 1   MAAILARKSLLALRARQFAISGQALQGSPFHGLRSSAHLYSTKREDEEREQLAKEMSKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           +SIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRY TGE
Sbjct: 61  NSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYATGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. ExPASy TrEMBL
Match: A0A6J1GJX8 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111455001 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.3e-117
Identity = 216/222 (97.30%), Postives = 219/222 (98.65%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MAAILARKSLLALRARQLA+SGQALQGS FH LRSSAHLYSTK+EDEEREQLAKE+SKDW
Sbjct: 1   MAAILARKSLLALRARQLAISGQALQGSRFHDLRSSAHLYSTKREDEEREQLAKEMSKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRY TGE
Sbjct: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYTTGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

BLAST of Bhi05G001095 vs. ExPASy TrEMBL
Match: A0A0A0K5Z9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G268510 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 6.6e-117
Identity = 216/222 (97.30%), Postives = 219/222 (98.65%), Query Frame = 0

Query: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLRSSAHLYSTKKEDEEREQLAKEISKDW 60
           MAAILARKSLLALRARQLAVSGQALQGSPFHGL+S+ HLYST K+DEEREQLAKEISKDW
Sbjct: 1   MAAILARKSLLALRARQLAVSGQALQGSPFHGLQST-HLYSTSKDDEEREQLAKEISKDW 60

Query: 61  SSIFERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120
           SSIFERSINTLFLTEMVRGL LTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE
Sbjct: 61  SSIFERSINTLFLTEMVRGLSLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYPTGE 120

Query: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180
           ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
Sbjct: 121 ERCIACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG 180

Query: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 223
           PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR
Sbjct: 181 PNFEFATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G16700.15.9e-11087.84Alpha-helical ferredoxin [more]
AT1G79010.18.5e-10986.94Alpha-helical ferredoxin [more]
ATCG01090.11.9e-1535.54NADPH dehydrogenases [more]
Match NameE-valueIdentityDescription
Q9FX838.3e-10987.84NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-B, mitochondrial OS=Arabid... [more]
P802692.4e-10886.90NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Solanum ... [more]
Q425991.2e-10786.94NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial OS=Arabid... [more]
O241431.2e-10484.78NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Nicotian... [more]
O212335.3e-7985.71NADH-ubiquinone oxidoreductase subunit 8 OS=Reclinomonas americana OX=48483 GN=N... [more]
Match NameE-valueIdentityDescription
A0A6J1C2E87.1e-11998.20NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Momordic... [more]
A0A6J1I8H71.2e-11897.30NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbit... [more]
A0A6J1F0I71.2e-11897.30NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial OS=Cucurbit... [more]
A0A6J1GJX81.3e-11797.30NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like OS=Cuc... [more]
A0A0A0K5Z96.6e-11797.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G268510 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010226NADH-quinone oxidoreductase, chain ITIGRFAMTIGR01971TIGR01971coord: 79..200
e-value: 1.8E-52
score: 174.0
IPR010226NADH-quinone oxidoreductase, chain IPANTHERPTHR10849NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIALcoord: 1..222
IPR010226NADH-quinone oxidoreductase, chain IHAMAPMF_01351NDH1_NuoIcoord: 70..206
score: 29.414911
IPR0178964Fe-4S ferredoxin-type, iron-sulphur binding domainPFAMPF12838Fer4_7coord: 122..176
e-value: 4.6E-13
score: 49.5
IPR0178964Fe-4S ferredoxin-type, iron-sulphur binding domainPROSITEPS513794FE4S_FER_2coord: 114..143
score: 9.994982
IPR0178964Fe-4S ferredoxin-type, iron-sulphur binding domainPROSITEPS513794FE4S_FER_2coord: 153..182
score: 10.742653
NoneNo IPR availableGENE3D3.30.70.3270coord: 96..221
e-value: 1.5E-46
score: 160.0
NoneNo IPR availablePANTHERPTHR10849:SF30SUBFAMILY NOT NAMEDcoord: 1..222
NoneNo IPR availableSUPERFAMILY548624Fe-4S ferredoxinscoord: 96..206
IPR0179004Fe-4S ferredoxin, iron-sulphur binding, conserved sitePROSITEPS001984FE4S_FER_1coord: 123..134
IPR0179004Fe-4S ferredoxin, iron-sulphur binding, conserved sitePROSITEPS001984FE4S_FER_1coord: 162..173

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M001095Bhi05M001095mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006120 mitochondrial electron transport, NADH to ubiquinone
biological_process GO:0032981 mitochondrial respiratory chain complex I assembly
cellular_component GO:0005747 mitochondrial respiratory chain complex I
cellular_component GO:0016020 membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003954 NADH dehydrogenase activity
molecular_function GO:0016651 oxidoreductase activity, acting on NAD(P)H