Bhi05G000865 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000865
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	chr5: 36035196 .. 36036070 (+)
RNA-Seq Expression	Bhi05G000865
Synteny	Bhi05G000865

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTACCAGAAACGTCCACTCGATTATCCTTTGTCGTTTGATCACTCTACTTTGCTCCGGCAGCTGACCTCCGGTAAGAAGAATCGGAACGCCTTGTCGTGGAGTTTGAGTTCTCGAGCTCGCTCTCTCACTGACCCTTTCCTTCAACCTCAGCAGCTTGTTAATCAGGTTGATTGAGTTTTGAATTTGATTCTGTGACTGTGATTGGTATGGATGATCGAGGATTCGATGTTTGATTGTTGACGGATGAGTGTTGAATGAAATTTTTGTGTTGTGCATTGTGAAATTGTGTTATGGACTATTCGGGTTTGGGTCTTTCGAAAGGATTGCATGGTTAAATTCCTGACATTGATCAACTAGTTGAGGATGTTATAAAACAATACAAGAAGTTTAAAGGTTTGTTGGTGGGTTGATACTAACTGTGTATGCGGATAATTACTCTTACCATCATTTCTGAATCCTTTTTTATTACCGGATCTTTTATTTCAACTCGCAGGTAGACTAGAATTGAAAGGATTGCCTCATTTCATATTCGGACAGTCCATGGGGGAAGCTATTACTTTGATAATGTACTTGAAAGAACCAAAGCTATGGGATGGAGTGGTTCTTGTGGCTCTCATGTGTAAAGTAAGTTCAAACAATTCTGCAATGGAAAAAGTGTTTTTTATATTGTTTTAGCTTGTACAGGTATGTGAATAGGGATTTCATTGCTTTTTAAGAGTGCAGACGATGTGAAACCGCCAAAACCAGTTGTTAAGGTCTTAAATCTAATGTCTAATGTTGTGCCAAAAGCCAAGCATCTCCCAAAGGTCGATCTTGGTGAACTAGCTCTCAGAGAAATGAAGAAAAGGAAACTGGTTCGTATGCCTTAA

mRNA sequence

ATGCTTTACCAGAAACGTCCACTCGATTATCCTTTGTCGTTTGATCACTCTACTTTGCTCCGGCAGCTGACCTCCGGTAAGAAGAATCGGAACGCCTTGTCGTGGAGTTTGAGTTCTCGAGCTCGCTCTCTCACTGACCCTTTCCTTCAACCTCAGCAGCTTGTTAATCAGTCCATGGGGGAAGCTATTACTTTGATAATGTACTTGAAAGAACCAAAGCTATGGGATGGAGTGGTTCTTGTGGCTCTCATGTGTAAAAGTGCAGACGATGTGAAACCGCCAAAACCAGTTGTTAAGGTCTTAAATCTAATGTCTAATGTTGTGCCAAAAGCCAAGCATCTCCCAAAGGTCGATCTTGGTGAACTAGCTCTCAGAGAAATGAAGAAAAGGAAACTGGTTCGTATGCCTTAA

Coding sequence (CDS)

ATGCTTTACCAGAAACGTCCACTCGATTATCCTTTGTCGTTTGATCACTCTACTTTGCTCCGGCAGCTGACCTCCGGTAAGAAGAATCGGAACGCCTTGTCGTGGAGTTTGAGTTCTCGAGCTCGCTCTCTCACTGACCCTTTCCTTCAACCTCAGCAGCTTGTTAATCAGTCCATGGGGGAAGCTATTACTTTGATAATGTACTTGAAAGAACCAAAGCTATGGGATGGAGTGGTTCTTGTGGCTCTCATGTGTAAAAGTGCAGACGATGTGAAACCGCCAAAACCAGTTGTTAAGGTCTTAAATCTAATGTCTAATGTTGTGCCAAAAGCCAAGCATCTCCCAAAGGTCGATCTTGGTGAACTAGCTCTCAGAGAAATGAAGAAAAGGAAACTGGTTCGTATGCCTTAA

Protein sequence

MLYQKRPLDYPLSFDHSTLLRQLTSGKKNRNALSWSLSSRARSLTDPFLQPQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPKAKHLPKVDLGELALREMKKRKLVRMP

Homology

[Close]

BLAST of Bhi05G000865 vs. TAIR 10
Match: AT1G77420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 4.3e-18
Identity = 45/82 (54.88%), Postives = 60/82 (73.17%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P+ L+ QSMG A+ L ++LKEP+ WDG++LVA MCK ++DVKPP  V+K L LMS + PK
Sbjct: 196 PRFLLGQSMGGAVALKIHLKEPQAWDGLILVAPMCKISEDVKPPPLVLKTLILMSTLFPK 255

Query: 111 AKHLPKVDLGELALREMKKRKL 133
           AK  PK DL +   R++ KRKL
Sbjct: 256 AKLFPKRDLSDFFFRDLSKRKL 277

[Close]

BLAST of Bhi05G000865 vs. TAIR 10
Match: AT5G16120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 1.8e-13
Identity = 37/82 (45.12%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P  L  QSMG A++L ++LK+P  W G VL+A MCK ADD+ PP  + ++L  ++NV+PK
Sbjct: 157 PSFLFGQSMGGAVSLKIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPK 216

Query: 111 AKHLPKVDLGELALREMKKRKL 133
            K +P+ DL E   R+++KR +
Sbjct: 217 HKLVPQKDLAEAGFRDIRKRDM 238

[Close]

BLAST of Bhi05G000865 vs. TAIR 10
Match: AT5G16120.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 1.8e-13
Identity = 37/82 (45.12%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P  L  QSMG A++L ++LK+P  W G VL+A MCK ADD+ PP  + ++L  ++NV+PK
Sbjct: 175 PSFLFGQSMGGAVSLKIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPK 234

Query: 111 AKHLPKVDLGELALREMKKRKL 133
            K +P+ DL E   R+++KR +
Sbjct: 235 HKLVPQKDLAEAGFRDIRKRDM 256

[Close]

BLAST of Bhi05G000865 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-12
Identity = 35/81 (43.21%), Postives = 55/81 (67.90%), Query Frame = 0


Query: 54  LVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPKAKH 113
           L+ +SMG A+ L+++ K+P+ WDG VLVA MCK A+++KP   V+ +L  +S V+P  K 
Sbjct: 115 LLGESMGGAVLLLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKI 174

Query: 114 LPKVDLGELALREMKKRKLVR 135
           +P  D+ E A ++ + RK VR
Sbjct: 175 IPGQDIIETAFKQPEIRKQVR 195

[Close]

BLAST of Bhi05G000865 vs. TAIR 10
Match: AT2G39410.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-11
Identity = 32/81 (39.51%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 54  LVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPKAKH 113
           ++ +SMG A+ L++  K P  WDG +LVA MCK A+++KP   V+ +L  + +++PK K 
Sbjct: 115 MLGESMGGAVVLLLGRKNPDFWDGAILVAPMCKIAEEMKPSPFVISILTKLISIIPKWKI 174

Query: 114 LPKVDLGELALREMKKRKLVR 135
           +P  D+ E++ +E + RK VR
Sbjct: 175 IPSQDIIEISYKEPEIRKQVR 195

[Close]

BLAST of Bhi05G000865 vs. ExPASy TrEMBL
Match: A0A5A7VHL1 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold979G001030 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 7.3e-26
Identity = 66/83 (79.52%), Postives = 74/83 (89.16%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P  ++ QSMG A+TL ++LKEPKLWDGVVLVA MCK ADDVK P+PV+KVLNLMSNVVPK
Sbjct: 240 PHFILGQSMGGAVTLKIHLKEPKLWDGVVLVAPMCKIADDVKLPEPVLKVLNLMSNVVPK 299

Query: 111 AKHLPKVDLGELALREMKKRKLV 134
           AK LPK+DLGELALREMKKRKLV
Sbjct: 300 AKLLPKIDLGELALREMKKRKLV 322

[Close]

BLAST of Bhi05G000865 vs. ExPASy TrEMBL
Match: A0A0A0KTB4 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G365670 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 7.3e-26
Identity = 65/82 (79.27%), Postives = 73/82 (89.02%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P  ++ QSMG A+TL ++LKEPKLWDGVVLVA MCK ADDVKPP+PV+KVLNLMSNVVPK
Sbjct: 214 PHFILGQSMGGAVTLKIHLKEPKLWDGVVLVAPMCKIADDVKPPEPVLKVLNLMSNVVPK 273

Query: 111 AKHLPKVDLGELALREMKKRKL 133
           AK LPK+DLGELALRE KKRKL
Sbjct: 274 AKLLPKIDLGELALRETKKRKL 295

[Close]

BLAST of Bhi05G000865 vs. ExPASy TrEMBL
Match: A0A5D3BYP9 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2119G00520 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.1e-25
Identity = 65/82 (79.27%), Postives = 73/82 (89.02%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P  ++ QSMG A+TL ++LKEPKLWDGVVLVA MCK ADDVK P+PV+KVLNLMSNVVPK
Sbjct: 250 PHFILGQSMGGAVTLKIHLKEPKLWDGVVLVAPMCKIADDVKLPEPVLKVLNLMSNVVPK 309

Query: 111 AKHLPKVDLGELALREMKKRKL 133
           AK LPK+DLGELALREMKKRKL
Sbjct: 310 AKLLPKIDLGELALREMKKRKL 331

[Close]

BLAST of Bhi05G000865 vs. ExPASy TrEMBL
Match: A0A1S3CPM0 (caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103503381 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.1e-25
Identity = 65/82 (79.27%), Postives = 73/82 (89.02%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P  ++ QSMG A+TL ++LKEPKLWDGVVLVA MCK ADDVK P+PV+KVLNLMSNVVPK
Sbjct: 250 PHFILGQSMGGAVTLKIHLKEPKLWDGVVLVAPMCKIADDVKLPEPVLKVLNLMSNVVPK 309

Query: 111 AKHLPKVDLGELALREMKKRKL 133
           AK LPK+DLGELALREMKKRKL
Sbjct: 310 AKLLPKIDLGELALREMKKRKL 331

[Close]

BLAST of Bhi05G000865 vs. ExPASy TrEMBL
Match: A0A6J1IBP0 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111472103 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 5.8e-23
Identity = 60/82 (73.17%), Postives = 70/82 (85.37%), Query Frame = 0


Query: 51  PQQLVNQSMGEAITLIMYLKEPKLWDGVVLVALMCKSADDVKPPKPVVKVLNLMSNVVPK 110
           P  ++ QSMG A+TL ++LKEPKLWDGVVLVA MCK ADDVKPP+PV+K+L LMS+VVPK
Sbjct: 214 PHFILGQSMGGAVTLKIHLKEPKLWDGVVLVAPMCKIADDVKPPEPVLKLLKLMSHVVPK 273

Query: 111 AKHLPKVDLGELALREMKKRKL 133
           AK LP VDLGELA+RE  KRKL
Sbjct: 274 AKLLPNVDLGELAIRETNKRKL 295

The following BLAST results are available for this feature:

BLAST of Bhi05G000865 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77420.1	4.3e-18	54.88	alpha/beta-Hydrolases superfamily protein	[more]
AT5G16120.1	1.8e-13	45.12	alpha/beta-Hydrolases superfamily protein	[more]
AT5G16120.2	1.8e-13	45.12	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39420.1	2.7e-12	43.21	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39410.2	1.3e-11	39.51	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Bhi05G000865 vs. TAIR 10


Match Name	E-value	Identity	Description

BLAST of Bhi05G000865 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VHL1	7.3e-26	79.52	Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A0A0KTB4	7.3e-26	79.27	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G365670...	[more]
A0A5D3BYP9	2.1e-25	79.27	Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3CPM0	2.1e-25	79.27	caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103503381 PE=4 SV=1	[more]
A0A6J1IBP0	5.8e-23	73.17	caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111472103 PE=4...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 51..116 e-value: 2.3E-6 score: 27.1
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 51..133
None	No IPR available	PANTHER	PTHR11614:SF94	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 51..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000865	Bhi05M000865	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity