Bhi05G000823 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000823
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionHemK methyltransferase family member 2
Locationchr5: 34609533 .. 34613479 (+)
RNA-Seq ExpressionBhi05G000823
SyntenyBhi05G000823
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAACCTAACCCGTTCCTTTTCCTATCTCTCTCCACAGCCCCAAAAATTCTCTTTCCCCATTCTCTTTCTTCCCATCTCCACCAGTTCGCCGCCGGCACCCTTTTTGCCGCAGCCGCAGCCGCCGGGTGGCCCAACCACACGTCGGTGCCATTATATGTGTCTCTTTATCGCAACTGCACTGCCACGCCGCCCCTCATTTACCTCCGCCACATGATTCGCTGCATTCTTCTGCCGGCCCAACTCTCAATCTTCCTCAATCTTCTGCAACTTTCTGTCGTCCGCCATTAATCACTTTCTTCAGCCTTCCAGTTGGTTTCCCCATTTTCTCGTAGGAGTTTTCTCGACCCTCCGTTCTCCTTTCCTGGTATTGTATCCGTTTCTTTTACTTTCTTTTGATTTCAATTGCTTTCTTTCCATTCTGATTGATTTTGGGTTTAATCGCTTAATTCCACTAATTTGTTTCGTAACGAAACGTGTTTGTGCACAATTATGTGGCTGTCGCTTTTTACTCATGTCGGAATCCGAATCGAGTTGTTTGATTTGCTTGATGGTTTCGAAGAAAATTGAATTGAGGGGTCTTTTATGGTGATTTGTTCTCGTTAATTTTGCTTCCGTTTTACTTCTTGACTTGTTTTACCTGTCTCTTTTCTCATGTTCTTATAAAAATGAGAAATCGGGGTCCCATTGTTGATGGAAGTGAAGGTTCTGATCCCGCCTTGTAGGAACATATTATTATACCGTGAACGAATTGCTTGGAGATTGTCTGGGAACCATCTGAGGAAAGAACTACTGAGAGTTGTGCACTCAGAAGTTGTGGTTGGGTTTTTTAAGAAAAAAAATTCAATGGTTCTTATATAAAAGATTGTTTCATCCCGCCACCACCAACCGCTTCCTATTAATCAGTGGAGTCCCTTCTGATATTGATTCCCATCCCCCATCCTTCTCCATGTTAGAACGTTGTTCATTCATTTATTATTGCACCTGGTTGCCTCTCCCCTTCAGGTGATCAAGAGACTTTGTCTTCTTGAGGTGTGCATTGCGCTTTGAAAATACTGGACATAGAGAGGGTTTTTTCTTTCTTTCTTCTTCATTATTATTATCTTTTAATCTTGCTTTGTGTTTCCTTCGCACATTTCACTCTTAGATACTTTCCTTGTTATAGAGAGAGTGCACTCAGCTATCTCCCTTGCTTGGATTTGGGTTTGACTTGCTGCGGTGTTTTCTTTTGGTTACAATTGTTTCTTATAATACACACACACATACGTATATGTAGAAAATCACTCATCTCTTATTATTCATATTTTCATGACAGGTCCGGCACATGTCCTCTTTCAGAATTGCACAAATTCACCTAGTGAGTTCTCATCGTGAGGTTTATGAACCGTGTGATGATTCGTTTGCTTTAGTTGATGCACTTTTAGCTGACCGAGTGAAATTGTTAAATCATCATCCAACATTGTGCTTGGAAGTTGGCTGTGGTAGTGGCTATGTTATAACATCTCTTGCTCTTATTCTGGGAAAGGAAGCTTCTGGAACCCATTATATTGCTACAGATATCAATCCGCATGCAATCAGAGTCACCCAGGAGACTCTAGAAGCACATGGGGTTCATGCAGAGTTGGTATCCACTGACATTGCATCTGGTTTGGAGCAGCGACTGGCTGGATCAGTTGATGTGTTGGTTGTGAATCCACCCTACGTTCCAACGCCAGAGGATGAAGTGGGTCGGGATGGAATTGCCTCTGCTTGGGCGGGAGGGGAAAATGGCCGAAGTGTCATCGATAGGATATTGCCAGTTGCAGATGTTCTTTTGTCTGAAAAGGGCTGGCTGTACATGGTTACTCTTACAGCGAATAATCCCTCTCAGATATGCCTTCAAATGAGAGAAAAGGGCTATGCTAGTAGAATCATTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTGATAAAGTTTTGGAAGGATGCTGATCTTCAAGTTGATGGAAAAGATTCAACACATAAAACAGGCCCTGGAAGGGTTGTGGAGTCTCTAATTTCACAACTTCCTCGTCTCTCTTTTTGGAGAAACAACAGCAACACTAACAGATGAATGAGTTATCAGAGTACGTTTCAAGACTTACTTGTCTCATCTTGTGATCCATTGTTTTTATTTTAATTTTCTGCGCTCTTGTGCTTTCCCTTTTACACTCGACCAAGTAGCTGTCACTGAAGGTTTCATTTTAGTGAAAGCAATAAGTTTTCATCCCTTAGTTTGCCGTCTTTCTTGTCCCATTATTGATGTCGTCTTTCTTATCCCATTATTGATGTCGTCTTTCTTATCCCATTATTGATGTCGTCTTTCTTATCCCATTATTGATGTTTCTCCTCACTGCCCGTTTACGCTAACTAGTGAAGCATCAAGTTCAATGGTGTTAACCTAATTATCTTGTCTAGGTTAGCTCATTCTTCCTTGTTTTAATTTCTTTCATATTATCTAACCATGCACGGAGACTTCGAAAGAAAAATTGCTTATATGTAGGCTTCATTTGATAACAATTTCATTTTTTGTTTTGTTTTTTTTTTTTTGCATTTTTTTGAAAATTTAAAAATTAAGTTTATAAATATACTACTTTGACCAATGAATTTCTCTGTTTTGTTATTCACTTTTTACTTATGTATTAAAAAACCAAGTCAAATTTTGATAGTTAAAAAAAATAGTTTTTAAAAAACTTTTTTGTTTTCAGAATTCGACAAAGAATTTAAATGGTGTTCTATATAAAGATGAAAACCATAGTAAGAAAATTAGTTAGAGACGAGCATAATTTTCAGAATCTAAAAGCAAGAAGTCAAATGGATATCAAATGGGGGTTGTAGTGATTCAATTTCAACCTCTAATCATCATGGGCTGAACTAGCGGTCAATAAGGGTCATAGGTTTAACAAAGAACTCATCTACTTAGGATTTAACTTATGAGTTTTCTTGACACTTAAATGTTGTAGTACAGATTCTCCCATGAGATTAGTTGAGGTGTATGCAAGCGGGTCTGCACAGACACTCCTTACCCCAAGAAAAAGAAAAAAAAAGTGATCTTTTAATTCCACACATTTGCAGCCTAAACTTTCACAGCCTTTTGTTACCTGCATGCAAGGGCCTTATATACCAATTGACCTCCAAAGTTTTGAAGTTAAAGCCAGTAGACGCATGCATACTTTTGCTTTGTGTATAATATTAGTTTTAACGTGGTAGATTGAAACTTCTATGGCGTATAGATTAATTTGATTAGCCTTCTTGGTTGTGCCTCTCTTGCAGGACGTGAGACTCATCTGCTCATCCTCCATTGTTACAAACAACAAGTCTAACCTTCCAACTTTGTGCATTCCACATGTGCATAGTGTTCGTTTATGAAGCACAGATATATATACGATTATACGATACAGATACGATCGGATACGACGATTTGTTAATTTCTTAAAAACTAAGATACAGATACGTCTAAGAATACGTCATTATATATATAATATAATAGTATATTATATATATAATATATTATTTCTAAAAAAAATGTTAAAGATATATTTTTTTTTCTTAAAAAAATCTAGGATATAAATTTAGTATACAAGTTAATAACAATAGCACAAAATAGAATACATATACTGAAATATAATAAAAAAAAGTAACATCTAAGATGTTGAAATACAATAGTTAATAAAATGCAATAGAAAAGTGATTCATATTACAAAGAAGAAGAAGATTAGAGGGAGAAATATGATGATAAATGAAGAACTTCTTAATTAAATTGTTGAAGATATTCAAATGATATATATTTTGATGGATTTTGTGGGGATTCAAATGTGAAGATGAATATTAGATGATTCTAGGGTTTGATCTTTTATTAATTTTTTTCTTTTTAGTTTTGGATTCGAGTAGTGAGCTTTTTAAATAGGTTGCCTAGTTTTAGATTGGG

mRNA sequence

TTAACCTAACCCGTTCCTTTTCCTATCTCTCTCCACAGCCCCAAAAATTCTCTTTCCCCATTCTCTTTCTTCCCATCTCCACCAGTTCGCCGCCGGCACCCTTTTTGCCGCAGCCGCAGCCGCCGGGTGGCCCAACCACACGTCGGTGCCATTATATGTGTCTCTTTATCGCAACTGCACTGCCACGCCGCCCCTCATTTACCTCCGCCACATGATTCGCTGCATTCTTCTGCCGGCCCAACTCTCAATCTTCCTCAATCTTCTGCAACTTTCTGTCGTCCGCCATTAATCACTTTCTTCAGCCTTCCAGTTGGTTTCCCCATTTTCTCGTAGGAGTTTTCTCGACCCTCCGTTCTCCTTTCCTGGTCCGGCACATGTCCTCTTTCAGAATTGCACAAATTCACCTAGTGAGTTCTCATCGTGAGGTTTATGAACCGTGTGATGATTCGTTTGCTTTAGTTGATGCACTTTTAGCTGACCGAGTGAAATTGTTAAATCATCATCCAACATTGTGCTTGGAAGTTGGCTGTGGTAGTGGCTATGTTATAACATCTCTTGCTCTTATTCTGGGAAAGGAAGCTTCTGGAACCCATTATATTGCTACAGATATCAATCCGCATGCAATCAGAGTCACCCAGGAGACTCTAGAAGCACATGGGGTTCATGCAGAGTTGGTATCCACTGACATTGCATCTGGTTTGGAGCAGCGACTGGCTGGATCAGTTGATGTGTTGGTTGTGAATCCACCCTACGTTCCAACGCCAGAGGATGAAGTGGGTCGGGATGGAATTGCCTCTGCTTGGGCGGGAGGGGAAAATGGCCGAAGTGTCATCGATAGGATATTGCCAGTTGCAGATGTTCTTTTGTCTGAAAAGGGCTGGCTGTACATGGTTACTCTTACAGCGAATAATCCCTCTCAGATATGCCTTCAAATGAGAGAAAAGGGCTATGCTAGTAGAATCATTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTGATAAAGTTTTGGAAGGATGCTGATCTTCAAGTTGATGGAAAAGATTCAACACATAAAACAGGCCCTGGAAGGGTTGTGGAGTCTCTAATTTCACAACTTCCTCGTCTCTCTTTTTGGAGAAACAACAGCAACACTAACAGATGAATGAGTTATCAGAGACGTGAGACTCATCTGCTCATCCTCCATTGTTACAAACAACAAGTCTAACCTTCCAACTTTGTGCATTCCACATGTGCATAGTGTTCGTTTATGAAGCACAGATATATATACGATTATACGATACAGATACGATCGGATACGACGATTTGTTAATTTCTTAAAAACTAAGATACAGATACGTCTAAGAATACGTCATTATATATATAATATAATAGTATATTATATATATAATATATTATTTCTAAAAAAAATGTTAAAGATATATTTTTTTTTCTTAAAAAAATCTAGGATATAAATTTAGTATACAAGTTAATAACAATAGCACAAAATAGAATACATATACTGAAATATAATAAAAAAAAGTAACATCTAAGATGTTGAAATACAATAGTTAATAAAATGCAATAGAAAAGTGATTCATATTACAAAGAAGAAGAAGATTAGAGGGAGAAATATGATGATAAATGAAGAACTTCTTAATTAAATTGTTGAAGATATTCAAATGATATATATTTTGATGGATTTTGTGGGGATTCAAATGTGAAGATGAATATTAGATGATTCTAGGGTTTGATCTTTTATTAATTTTTTTCTTTTTAGTTTTGGATTCGAGTAGTGAGCTTTTTAAATAGGTTGCCTAGTTTTAGATTGGG

Coding sequence (CDS)

ATGTCCTCTTTCAGAATTGCACAAATTCACCTAGTGAGTTCTCATCGTGAGGTTTATGAACCGTGTGATGATTCGTTTGCTTTAGTTGATGCACTTTTAGCTGACCGAGTGAAATTGTTAAATCATCATCCAACATTGTGCTTGGAAGTTGGCTGTGGTAGTGGCTATGTTATAACATCTCTTGCTCTTATTCTGGGAAAGGAAGCTTCTGGAACCCATTATATTGCTACAGATATCAATCCGCATGCAATCAGAGTCACCCAGGAGACTCTAGAAGCACATGGGGTTCATGCAGAGTTGGTATCCACTGACATTGCATCTGGTTTGGAGCAGCGACTGGCTGGATCAGTTGATGTGTTGGTTGTGAATCCACCCTACGTTCCAACGCCAGAGGATGAAGTGGGTCGGGATGGAATTGCCTCTGCTTGGGCGGGAGGGGAAAATGGCCGAAGTGTCATCGATAGGATATTGCCAGTTGCAGATGTTCTTTTGTCTGAAAAGGGCTGGCTGTACATGGTTACTCTTACAGCGAATAATCCCTCTCAGATATGCCTTCAAATGAGAGAAAAGGGCTATGCTAGTAGAATCATTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTGATAAAGTTTTGGAAGGATGCTGATCTTCAAGTTGATGGAAAAGATTCAACACATAAAACAGGCCCTGGAAGGGTTGTGGAGTCTCTAATTTCACAACTTCCTCGTCTCTCTTTTTGGAGAAACAACAGCAACACTAACAGATGA

Protein sequence

MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITSLALILGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVLVVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNPSQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLISQLPRLSFWRNNSNTNR
Homology
BLAST of Bhi05G000823 vs. TAIR 10
Match: AT3G13440.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 1.8e-94
Identity = 163/245 (66.53%), Postives = 202/245 (82.45%), Query Frame = 0

Query: 5   RIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITSLALI 64
           RIA I LVSSHREVYEPCDDSFALVDALLADR  L+ H+P +C+E+GCGSGYVITSL L+
Sbjct: 36  RIAAIRLVSSHREVYEPCDDSFALVDALLADRTNLIEHNPKICMEIGCGSGYVITSLILL 95

Query: 65  LGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVLVVNP 124
           L  E  G HY+A D NP A RVT+ETLEAHGV+A+++  D+A+GLE+RLAGSVDV+VVNP
Sbjct: 96  LQNEVPGVHYLAIDTNPIATRVTKETLEAHGVNADVICADLATGLEKRLAGSVDVIVVNP 155

Query: 125 PYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNPSQIC 184
           PYVPTPE EVG +GIASAWAGGENGR+VID+ILPV D+LLSEKGW Y+VTLT+N P++IC
Sbjct: 156 PYVPTPEYEVGMEGIASAWAGGENGRTVIDKILPVVDLLLSEKGWFYLVTLTSNFPAEIC 215

Query: 185 LQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLISQLPR 244
           L MR+KGYASRI+VQRSTEEE+L ++KFW+D D +   K+++  +   +  +SL S + +
Sbjct: 216 LMMRKKGYASRIVVQRSTEEENLVILKFWRDKDEESQDKETSSSSFVSQFSKSLSSLMEK 275

Query: 245 LSFWR 250
              WR
Sbjct: 276 Q--WR 278

BLAST of Bhi05G000823 vs. ExPASy Swiss-Prot
Match: Q6SKR2 (Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 9.2e-43
Identity = 94/199 (47.24%), Postives = 122/199 (61.31%), Query Frame = 0

Query: 16  REVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITSLALILGKEASGTHYI 75
           R+VYEP +D+F L+DAL A   +L      +CLEVG GSG V   LA ++G  A    Y+
Sbjct: 20  RDVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGAGSGVVSAFLASMIGPRAL---YM 79

Query: 76  ATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVLVVNPPYVPTPEDEVG 135
            TDINP A   T ET   + VH + V TD+  GL  RL G VD+LV NPPYV TP +EVG
Sbjct: 80  CTDINPEAAACTLETARCNRVHVQPVITDLVHGLLPRLKGKVDLLVFNPPYVVTPPEEVG 139

Query: 136 RDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNPSQICLQMREKGYASR 195
             GI +AWAGG NGR V+DR  P+A  LLS +G  Y+VT+  NNP +I   M+ +G    
Sbjct: 140 SRGIEAAWAGGRNGREVMDRFFPLAPELLSPRGLFYLVTVKENNPEEIFKTMKTRGLQGT 199

Query: 196 IIVQRSTEEESLHVIKFWK 215
             + R   +E+L V++F K
Sbjct: 200 TALCRQAGQEALSVLRFSK 213

BLAST of Bhi05G000823 vs. ExPASy Swiss-Prot
Match: Q9Y5N5 (Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4)

HSP 1 Score: 172.6 bits (436), Expect = 5.9e-42
Identity = 93/198 (46.97%), Postives = 119/198 (60.10%), Query Frame = 0

Query: 17  EVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITSLALILGKEASGTHYIA 76
           +VYEP +D+F L+DAL A   +L      +CLEVG GSG V   LA ++G +A    Y+ 
Sbjct: 21  DVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGSGSGVVSAFLASMIGPQAL---YMC 80

Query: 77  TDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVLVVNPPYVPTPEDEVGR 136
           TDINP A   T ET   + VH + V TD+  GL  RL   VD+LV NPPYV TP  EVG 
Sbjct: 81  TDINPEAAACTLETARCNKVHIQPVITDLVKGLLPRLTEKVDLLVFNPPYVVTPPQEVGS 140

Query: 137 DGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNPSQICLQMREKGYASRI 196
            GI +AWAGG NGR V+DR  P+   LLS +G  Y+VT+  NNP +I   M+ KG     
Sbjct: 141 HGIEAAWAGGRNGREVMDRFFPLVPDLLSPRGLFYLVTIKENNPEEILKIMKTKGLQGTT 200

Query: 197 IVQRSTEEESLHVIKFWK 215
            + R   +E+L V+KF K
Sbjct: 201 ALSRQAGQETLSVLKFTK 213

BLAST of Bhi05G000823 vs. ExPASy Swiss-Prot
Match: Q9UT94 (eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mtq2 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.1e-22
Identity = 70/211 (33.18%), Postives = 110/211 (52.13%), Query Frame = 0

Query: 16  REVYEPCDDSFALVDALLADRVKL---LNHHPTLCLEVGCGSGYVITSLALILGKEASGT 75
           ++VYEP +D+FAL+DAL  D  KL         L  E+GCGSG   + L   + K     
Sbjct: 16  QDVYEPAEDTFALLDALEKDAKKLRQMAEMKNLLTAEIGCGSGCASSFLKSGILKNKPIV 75

Query: 76  HYIATDINPHAIRVTQET-LEAHGVHAE------LVSTDIASGLEQRLAGSVDVLVVNPP 135
           H++ +DI+  A R ++ T L    ++ +       V T    G+  RL   VD+L+ NPP
Sbjct: 76  HFM-SDISNSACRASKITALNNRELYKDDNGLFITVQTSFLDGI--RLGNGVDILIFNPP 135

Query: 136 YVPTPEDEVGRDG--IASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNPSQI 195
           YVPT  +E+  +   IASAWAGG +G  V   +L     +LS+ G  YMV +  N    I
Sbjct: 136 YVPTEFEEIPSEAATIASAWAGGTDGMDVTSTLLNQLKDILSQDGVFYMVAVARNKLHSI 195

Query: 196 CLQMREKGYASRIIVQRSTEEESLHVIKFWK 215
           C  +++ G+     ++R    E+L +++ ++
Sbjct: 196 CEILQKDGFIVNETLKRKAGRETLSILRIYR 223

BLAST of Bhi05G000823 vs. ExPASy Swiss-Prot
Match: Q58338 (Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0928 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.1e-17
Identity = 54/200 (27.00%), Postives = 98/200 (49.00%), Query Frame = 0

Query: 15  HREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITSLALILGKEASGTHY 74
           H EVYEP +DS  L+  L+  + K +       LE+G G+G +  + A     +      
Sbjct: 13  HPEVYEPAEDSILLLKNLVDVKNKDV-------LEIGVGTGLISIACA-----KKGAKKI 72

Query: 75  IATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVLVVNPPYVPTPEDEV 134
           +  DINP+A+++ +E  + + V+     +D    L + + G  DV++ NPPY+PT EDE 
Sbjct: 73  VGVDINPYAVKLAKENAKLNNVNISFFESD----LFENVTGKFDVILFNPPYLPTSEDEK 132

Query: 135 GRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNPSQICLQMREKGYAS 194
               +  A+ GG++GR ++DR +      L + G + ++  +     +   +++  G+  
Sbjct: 133 IDSYLNFAFDGGKDGREILDRFIYELPNYLKKGGVVQILQSSLTGEKETINKLKPLGFKV 192

Query: 195 RIIVQRSTEEESLHVIKFWK 215
            I  +     E L VI  W+
Sbjct: 193 EISARLKVPFEELMVINAWR 196

BLAST of Bhi05G000823 vs. ExPASy Swiss-Prot
Match: O28490 (Putative protein N5-glutamine methyltransferase AF_1784 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1784 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.8e-14
Identity = 61/199 (30.65%), Postives = 98/199 (49.25%), Query Frame = 0

Query: 18  VYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITSLALILGKEASGTHYIAT 77
           +YEP +DS  L++A L +            +EVG GSG+V   L      +      + T
Sbjct: 2   IYEPAEDSELLLEAALREL-----KPDDEVIEVGAGSGFVAERL------KGKCKCILTT 61

Query: 78  DINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVLVVNPPYVPTPEDEVGR- 137
           DI+P A       +E      ++V TDIA G+ ++ +    +++ NPPYV   EDE+ R 
Sbjct: 62  DISPFA------AMELRRKGLDVVMTDIAKGIRKKFS----LVLFNPPYVEL-EDELRRG 121

Query: 138 DGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNPSQICLQMREKGYASRI 197
           D +  A  GG+ G  VI + L   D +++E+G   ++  + N P    L + E+G+   I
Sbjct: 122 DWLDVAIDGGKKGVKVIKKFLDSLDEIMAERGRAILIASSQNEPDVFDL-IDERGFRYEI 177

Query: 198 IVQRSTEEESLHVIKFWKD 216
           + +R    E L  IK WK+
Sbjct: 182 VGERGLFFEKLFAIKIWKE 177

BLAST of Bhi05G000823 vs. ExPASy TrEMBL
Match: A0A5D3C4M9 (HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001750 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.6e-138
Identity = 245/256 (95.70%), Postives = 252/256 (98.44%), Query Frame = 0

Query: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITS 60
           MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNH+PTLCLE GCGSGYVITS
Sbjct: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVL 120
           LALILGKEAS THYIATDINPHAIRVTQETLEAHG+HAELV TD+ASGLE+RLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVG+DGIASAWAGGENGRSVIDRILPVADVLLS+KGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240
            QICLQMREKGYASRI+VQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTNR 257
           QLPRLSFWRNNSNTNR
Sbjct: 241 QLPRLSFWRNNSNTNR 256

BLAST of Bhi05G000823 vs. ExPASy TrEMBL
Match: A0A5A7SL12 (HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001760 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.7e-137
Identity = 244/256 (95.31%), Postives = 251/256 (98.05%), Query Frame = 0

Query: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITS 60
           MSSFR AQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNH+PTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVL 120
           LALILGKEAS THYIATDINPHAIRVTQETLEAHG+HAELV TD+ASGLE+RLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVG+DGIASAWAGGENGRSVIDRILPVADVLLS+KGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240
            QICLQMREKGYASRI+VQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTNR 257
           QLPRLSFWRNNSNTNR
Sbjct: 241 QLPRLSFWRNNSNTNR 256

BLAST of Bhi05G000823 vs. ExPASy TrEMBL
Match: E5GCR1 (N6-DNA-methyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.7e-137
Identity = 244/256 (95.31%), Postives = 251/256 (98.05%), Query Frame = 0

Query: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITS 60
           MSSFR AQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNH+PTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVL 120
           LALILGKEAS THYIATDINPHAIRVTQETLEAHG+HAELV TD+ASGLE+RLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVG+DGIASAWAGGENGRSVIDRILPVADVLLS+KGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240
            QICLQMREKGYASRI+VQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTNR 257
           QLPRLSFWRNNSNTNR
Sbjct: 241 QLPRLSFWRNNSNTNR 256

BLAST of Bhi05G000823 vs. ExPASy TrEMBL
Match: A0A1S3C053 (hemK methyltransferase family member 2 OS=Cucumis melo OX=3656 GN=LOC103495363 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.7e-137
Identity = 244/256 (95.31%), Postives = 251/256 (98.05%), Query Frame = 0

Query: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITS 60
           MSSFR AQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNH+PTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVL 120
           LALILGKEAS THYIATDINPHAIRVTQETLEAHG+HAELV TD+ASGLE+RLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVG+DGIASAWAGGENGRSVIDRILPVADVLLS+KGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240
            QICLQMREKGYASRI+VQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTNR 257
           QLPRLSFWRNNSNTNR
Sbjct: 241 QLPRLSFWRNNSNTNR 256

BLAST of Bhi05G000823 vs. ExPASy TrEMBL
Match: A0A6J1I5Y4 (hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470989 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 3.9e-137
Identity = 244/256 (95.31%), Postives = 250/256 (97.66%), Query Frame = 0

Query: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITS 60
           MSSFR AQIHLVSSHREVYEPCDDSFALVDALLA+RV LLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALILGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVL 120
           LAL+LGKEASG HYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLE+RLAGSVDVL
Sbjct: 61  LALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRIL VADVLLS+KGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILLVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240
           SQICLQMREKGYASRI+VQRSTEEESLHVIKFWKDADLQVDGK+STHKTGP RVVESLIS
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKESTHKTGPARVVESLIS 240

Query: 241 QLPRLSFWRNNSNTNR 257
           Q PRLSFWRNNSNTNR
Sbjct: 241 QFPRLSFWRNNSNTNR 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G13440.11.8e-9466.53S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
Match NameE-valueIdentityDescription
Q6SKR29.2e-4347.24Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1[more]
Q9Y5N55.9e-4246.97Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4[more]
Q9UT943.1e-2233.18eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (stra... [more]
Q583385.1e-1727.00Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jan... [more]
O284901.8e-1430.65Putative protein N5-glutamine methyltransferase AF_1784 OS=Archaeoglobus fulgidu... [more]
Match NameE-valueIdentityDescription
A0A5D3C4M94.6e-13895.70HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5A7SL121.7e-13795.31HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
E5GCR11.7e-13795.31N6-DNA-methyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3C0531.7e-13795.31hemK methyltransferase family member 2 OS=Cucumis melo OX=3656 GN=LOC103495363 P... [more]
A0A6J1I5Y43.9e-13795.31hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 16..221
e-value: 8.4E-44
score: 151.2
NoneNo IPR availablePANTHERPTHR45875METHYLTRANSFERASE N6AMT1coord: 4..221
NoneNo IPR availablePANTHERPTHR45875:SF5BNAC01G37640D PROTEINcoord: 4..221
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 48..132
e-value: 5.74781E-8
score: 47.8099
IPR004557Eukaryotic/archaeal PrmC-relatedTIGRFAMTIGR00537TIGR00537coord: 18..213
e-value: 4.6E-28
score: 96.1
IPR007848Methyltransferase small domainPFAMPF05175MTScoord: 43..128
e-value: 1.9E-10
score: 40.6
IPR002052DNA methylase, N-6 adenine-specific, conserved sitePROSITEPS00092N6_MTASEcoord: 120..126
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 26..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000823Bhi05M000823mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006479 protein methylation
biological_process GO:0032259 methylation
cellular_component GO:0035657 eRF1 methyltransferase complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008276 protein methyltransferase activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity