Bhi05G000648 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000648
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionENTH domain-containing protein
Locationchr5: 26897381 .. 26900036 (-)
RNA-Seq ExpressionBhi05G000648
SyntenyBhi05G000648
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCAAGCAAAATTAGAAGAGCATTAGGAGCTGTGAAAGACAAGACAAGCATAGGTTTGGCCAAAGTTGGAAGCAGCACTTCCTTGTCGGATTTGGAAGTAGCCATTGTTAAAGCTACAAGACATGAAGAATATCCAGCTGAAGAACGGCATGTGCGCGAGATTCTTAGTCTCACGTGCTATTCACGTGCGTATATAAGCGCGTGTGTTAACACGCTCTCAAGGAGATTGAACAAGACAAAGAATTGGACAGTAGCACTTAAAACTTTAATGTTAATACAACGTTTGGTGTCGGAAGGGGATCCAGCTTATGAACAAGAGATATTTTTTTCTACAAGACGTGGGACAAGATTCTTAAATATGTCTGATTTTAGAGATACTTCTCAATCAAATTCATGGGATTATTCTGCTTTTGTTCGTACTTTTGCTTTGTATCTTGATGAAAGGCTTGAGTTTAGGATGCAAAGTAGAAGGGGAAAAAGGAGTGCATTTGGATTGGATGATGAAGAAGGCCAACAACAACAAATTAATAATAATAATAATAATAATAATGATAATAAGAACGACGATGACAATAATGATGAGGATGACAATAGCAAAGCCATTGTCGTAAGAGCCACACCCATACGAGATATGACGTCTGAACAAATATTCTCGAGGACACAACATTTGCAACAGCTTCTTGAACGCTTTTTGGCTTGTCGTCCTACAGGTTCATTTCATTTTAATACTACTTTGATCTTTGTACGTATTTTTACTTTGGTTTATTTTGGTTAATGTACTTTCAAACTTTTTTCGTTCTTTGTTTTCTTTCACAATGTTCATTTTGGTGTCCTTATCATTTTTATTCTTATTTTCAATAATCAATTTTATACCTAATAGAAACTATTTAACACAACTTCATGGATACATTTTAAGAAATGTAGTGAATGAAGTTTGATATTAAAATTTTGATTGGAAAAAATAAAATAAAAATAGAAATAGAAATGAAAGACCAAAATGTCACTTTTTAAAGATACAAAATTAGAAATACATGTACCAAAATGAATCAAAGTTGAAAGTAGAGATATCAAAGTAGTATTTAAGCCTTTGAAATGAATATTCGTACTTCTTGAAATTCTATAACATAATCATTTTTTTCTTCTTTACTTTGCATCTAATATATGCTTACTTATATATGCTCATATTAGCACATTAATTAATGACATACTTGAAGTCACATATCCATTATTTATTAGAAGTAAACTGAATGTTAAGATCTAAAATGGTTACTTTTATTACATTTTCTTAAGTTTATAAACTCTAATAAACATTTTGAATGTTTGAGGCAAAATTAGCAAAATGGAATTCAATTTCACATCTTCGCTTATCTGTATTGATTTAAAAACTGTCTAAAAAGACCAAGAGAACTTGTCCCTCCAACAAAAATACAAGTAAAAATCAGTATTTAGCTACTAAATTTTTGTGAAATTGATTTATTATGTTGTTAATTGTGTGTTTATTTCTACATAAATATAGGTGCAGCGAAGAACAACAGGGTGGTATTAGTAGCTTTATACCCAATCGTAAGGGAAAGTTTCCAAATATATTATGACATAACAGAAATTATGGGGATCTTAATTGATCGTTTCATGGAATTAAACGTTCATGATTGTGTCAAAGTTTATGAGATTTTCTGTCGAGTTGGGAAGCAATTTGATGAACTAGAATTGTTCTATTCTTGGTGTAGAACAATTGGAATTGCTCGTTCTTCTGAATATCCTGATGTTGAAAAAATTACTCCTAAAAAACTTGAAGTAATGGATGAATTCATCAAAGACAAATCTGCCTTAGCTCAATGCCAAACGGGCAGAGGAAAAAATCAACTTCAAATAGAAATAACAGAGGAAGTAAAAAAAGAAGAAGAAATAGATATGAATTCAATTAAAGCACTTCCACCACCTGAGGATTTCAAAGAGGAAGACATTACAGCCATAGAAGTGAAAGAAGAAGAGAATGAAATTAAAGAAGATGTAATTAAGAAGGATGAAAATCCTTTAATTGGTGATTTGTTAAACCTTGGTGAAGAACATGCAATGATAAGAGAAGAAAATGCTGATAAATTAGCATTAGCATTATTTGATGGAATTGGTCCAGTTACTGATTCTTCTTCAGTAAAAACATTACCATGGCAAGCATTTAATGATGATTCACAAGATTGGGAAACAACTTTAGTTCAATCAGCAAGTAATTTATCAAATCAAAGAGCTGATCTTGGTGGAGGATTTGATATGCTTCTTTTGGATGGAATGTATAAACAAACAACAATGATGTCAACAATGGCTGGGTCAGGTTATGGAGTAAGTGGTAGTGCAAGTAGTATGGCACTTGGGTCTGCAGGGAGACCAGCAATGCTCGCTTTGCCAGCCCCTCCGACGATCACCGAAGGCGGTGGGGTGAGCTCGTCATCGGTCGTGAGTGACCCATTTGCTGCATCGATGGCGGTGGCACCTCCCCCGTACGTTCAGATGTCGGAAATGGAGAGGAAACAGAAGCTGTTGGTGGAGGAGCAACTCATGTGGCAGCAATATGCAAGAGATGGGAGACAGATACAGCATAACCCTTATACAGGAGGTTTCACACACAGTTACTAGAGTTTTGGCTTCACTTGTTT

mRNA sequence

ATGGCACCAAGCAAAATTAGAAGAGCATTAGGAGCTGTGAAAGACAAGACAAGCATAGGTTTGGCCAAAGTTGGAAGCAGCACTTCCTTGTCGGATTTGGAAGTAGCCATTGTTAAAGCTACAAGACATGAAGAATATCCAGCTGAAGAACGGCATGTGCGCGAGATTCTTAGTCTCACGTGCTATTCACGTGCGTATATAAGCGCGTGTGTTAACACGCTCTCAAGGAGATTGAACAAGACAAAGAATTGGACAGTAGCACTTAAAACTTTAATGTTAATACAACGTTTGGTGTCGGAAGGGGATCCAGCTTATGAACAAGAGATATTTTTTTCTACAAGACGTGGGACAAGATTCTTAAATATGTCTGATTTTAGAGATACTTCTCAATCAAATTCATGGGATTATTCTGCTTTTGTTCGTACTTTTGCTTTGTATCTTGATGAAAGGCTTGAGTTTAGGATGCAAAGTAGAAGGGGAAAAAGGAGTGCATTTGGATTGGATGATGAAGAAGGCCAACAACAACAAATTAATAATAATAATAATAATAATAATGATAATAAGAACGACGATGACAATAATGATGAGGATGACAATAGCAAAGCCATTGTCGTAAGAGCCACACCCATACGAGATATGACGTCTGAACAAATATTCTCGAGGACACAACATTTGCAACAGCTTCTTGAACGCTTTTTGGCTTGTCGTCCTACAGGTGCAGCGAAGAACAACAGGGTGGTATTAGTAGCTTTATACCCAATCGTAAGGGAAAGTTTCCAAATATATTATGACATAACAGAAATTATGGGGATCTTAATTGATCGTTTCATGGAATTAAACGTTCATGATTGTGTCAAAGTTTATGAGATTTTCTGTCGAGTTGGGAAGCAATTTGATGAACTAGAATTGTTCTATTCTTGGTGTAGAACAATTGGAATTGCTCGTTCTTCTGAATATCCTGATGTTGAAAAAATTACTCCTAAAAAACTTGAAGTAATGGATGAATTCATCAAAGACAAATCTGCCTTAGCTCAATGCCAAACGGGCAGAGGAAAAAATCAACTTCAAATAGAAATAACAGAGGAAGTAAAAAAAGAAGAAGAAATAGATATGAATTCAATTAAAGCACTTCCACCACCTGAGGATTTCAAAGAGGAAGACATTACAGCCATAGAAGTGAAAGAAGAAGAGAATGAAATTAAAGAAGATGTAATTAAGAAGGATGAAAATCCTTTAATTGGTGATTTGTTAAACCTTGGTGAAGAACATGCAATGATAAGAGAAGAAAATGCTGATAAATTAGCATTAGCATTATTTGATGGAATTGGTCCAGTTACTGATTCTTCTTCAGTAAAAACATTACCATGGCAAGCATTTAATGATGATTCACAAGATTGGGAAACAACTTTAGTTCAATCAGCAAGTAATTTATCAAATCAAAGAGCTGATCTTGGTGGAGGATTTGATATGCTTCTTTTGGATGGAATGTATAAACAAACAACAATGATGTCAACAATGGCTGGGTCAGGTTATGGAGTAAGTGGTAGTGCAAGTAGTATGGCACTTGGGTCTGCAGGGAGACCAGCAATGCTCGCTTTGCCAGCCCCTCCGACGATCACCGAAGGCGGTGGGGTGAGCTCGTCATCGGTCGTGAGTGACCCATTTGCTGCATCGATGGCGGTGGCACCTCCCCCGTACGTTCAGATGTCGGAAATGGAGAGGAAACAGAAGCTGTTGGTGGAGGAGCAACTCATGTGGCAGCAATATGCAAGAGATGGGAGACAGATACAGCATAACCCTTATACAGGAGGTTTCACACACAGTTACTAGAGTTTTGGCTTCACTTGTTT

Coding sequence (CDS)

ATGGCACCAAGCAAAATTAGAAGAGCATTAGGAGCTGTGAAAGACAAGACAAGCATAGGTTTGGCCAAAGTTGGAAGCAGCACTTCCTTGTCGGATTTGGAAGTAGCCATTGTTAAAGCTACAAGACATGAAGAATATCCAGCTGAAGAACGGCATGTGCGCGAGATTCTTAGTCTCACGTGCTATTCACGTGCGTATATAAGCGCGTGTGTTAACACGCTCTCAAGGAGATTGAACAAGACAAAGAATTGGACAGTAGCACTTAAAACTTTAATGTTAATACAACGTTTGGTGTCGGAAGGGGATCCAGCTTATGAACAAGAGATATTTTTTTCTACAAGACGTGGGACAAGATTCTTAAATATGTCTGATTTTAGAGATACTTCTCAATCAAATTCATGGGATTATTCTGCTTTTGTTCGTACTTTTGCTTTGTATCTTGATGAAAGGCTTGAGTTTAGGATGCAAAGTAGAAGGGGAAAAAGGAGTGCATTTGGATTGGATGATGAAGAAGGCCAACAACAACAAATTAATAATAATAATAATAATAATAATGATAATAAGAACGACGATGACAATAATGATGAGGATGACAATAGCAAAGCCATTGTCGTAAGAGCCACACCCATACGAGATATGACGTCTGAACAAATATTCTCGAGGACACAACATTTGCAACAGCTTCTTGAACGCTTTTTGGCTTGTCGTCCTACAGGTGCAGCGAAGAACAACAGGGTGGTATTAGTAGCTTTATACCCAATCGTAAGGGAAAGTTTCCAAATATATTATGACATAACAGAAATTATGGGGATCTTAATTGATCGTTTCATGGAATTAAACGTTCATGATTGTGTCAAAGTTTATGAGATTTTCTGTCGAGTTGGGAAGCAATTTGATGAACTAGAATTGTTCTATTCTTGGTGTAGAACAATTGGAATTGCTCGTTCTTCTGAATATCCTGATGTTGAAAAAATTACTCCTAAAAAACTTGAAGTAATGGATGAATTCATCAAAGACAAATCTGCCTTAGCTCAATGCCAAACGGGCAGAGGAAAAAATCAACTTCAAATAGAAATAACAGAGGAAGTAAAAAAAGAAGAAGAAATAGATATGAATTCAATTAAAGCACTTCCACCACCTGAGGATTTCAAAGAGGAAGACATTACAGCCATAGAAGTGAAAGAAGAAGAGAATGAAATTAAAGAAGATGTAATTAAGAAGGATGAAAATCCTTTAATTGGTGATTTGTTAAACCTTGGTGAAGAACATGCAATGATAAGAGAAGAAAATGCTGATAAATTAGCATTAGCATTATTTGATGGAATTGGTCCAGTTACTGATTCTTCTTCAGTAAAAACATTACCATGGCAAGCATTTAATGATGATTCACAAGATTGGGAAACAACTTTAGTTCAATCAGCAAGTAATTTATCAAATCAAAGAGCTGATCTTGGTGGAGGATTTGATATGCTTCTTTTGGATGGAATGTATAAACAAACAACAATGATGTCAACAATGGCTGGGTCAGGTTATGGAGTAAGTGGTAGTGCAAGTAGTATGGCACTTGGGTCTGCAGGGAGACCAGCAATGCTCGCTTTGCCAGCCCCTCCGACGATCACCGAAGGCGGTGGGGTGAGCTCGTCATCGGTCGTGAGTGACCCATTTGCTGCATCGATGGCGGTGGCACCTCCCCCGTACGTTCAGATGTCGGAAATGGAGAGGAAACAGAAGCTGTTGGTGGAGGAGCAACTCATGTGGCAGCAATATGCAAGAGATGGGAGACAGATACAGCATAACCCTTATACAGGAGGTTTCACACACAGTTACTAG

Protein sequence

MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFLNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNNNNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNPYTGGFTHSY
Homology
BLAST of Bhi05G000648 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 671.8 bits (1732), Expect = 5.3e-193
Identity = 374/605 (61.82%), Postives = 462/605 (76.36%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSTSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE+++REILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+ EGD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINN 180
           LNMSDFRD S+SNSWDYSAFVRT+ALYLDERL+FRMQ+R GKR  + +  E  +++Q   
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQ--- 180

Query: 181 NNNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
                        +    D S AIVVR+ PI +M +EQIF R QHLQQLL+RFLACRPTG
Sbjct: 181 -------------DQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            A+NNRVV+VALYPIV+ESFQIYYD+TEIMGILI+RFMEL++ D +KVY+IFCRV KQF+
Sbjct: 241 NARNNRVVIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFE 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIE- 360
           EL+ FYSWC+ +GIARSSEYP++EKIT KKL++MDEFI+DKSAL   +  +       E 
Sbjct: 301 ELDQFYSWCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADED 360

Query: 361 ----ITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIG 420
                TEEV +E+E DMN+IKALP P   +E+D+      + E E KE+VI + +   +G
Sbjct: 361 DDEARTEEVNEEQE-DMNAIKALPEPPPKEEDDV------KPEEEAKEEVIIEKKQEEMG 420

Query: 421 DLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLP-WQAFNDDSQDWETTLVQS 480
           DLL+LG  +     +  D LALALFD  GP    S  ++ P W+AF DDS DWET LVQ+
Sbjct: 421 DLLDLGNTNGGEAGQAGDSLALALFD--GPYASGSGSESGPGWEAFKDDSADWETALVQT 480

Query: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSASSMALGSAGRPA--M 540
           A+NLS Q+++LGGGFDMLLL+GMY+   + + +   + YG SGSASSMA GSAGRPA  M
Sbjct: 481 ATNLSGQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATM 540

Query: 541 LALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYA 596
           LALPAP T     G  +S V  DPFAAS+ VAPP YVQM++ME+KQ++L+EEQ+MW QY+
Sbjct: 541 LALPAPSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYS 580

BLAST of Bhi05G000648 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 651.4 bits (1679), Expect = 7.4e-187
Identity = 361/604 (59.77%), Postives = 464/604 (76.82%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-STSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE++++REIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+++GD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINN 180
           LNMSDFRD SQS+SWDYSAFVRT+ALYLDERL++RMQ RRGK+ + G    +G   + ++
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDD 180

Query: 181 NNNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
           +   +ND +           SKAIVV++ P+ +M +E+IF+R QHLQQLL+RFLACRPTG
Sbjct: 181 HRGTSNDIR-----------SKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            AKNNRVV+VA+YPIV+ESFQ+YY+ITEIMG+LI+RFMEL++HD +KVYEIFCRV KQFD
Sbjct: 241 NAKNNRVVIVAMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFD 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEI 360
           EL+ FY WC+ + +ARSSEYP++EKIT KKL++MDEFI+DKSALA  QT +  ++   + 
Sbjct: 301 ELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDEFIRDKSALA-AQTTKSSSKRSNKS 360

Query: 361 TEEVKKEEEI-----DMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIG 420
            EE  K E I     D+NSIKALP PE  +EE+    E  E + +++E V ++D+    G
Sbjct: 361 EEEESKTEYIQENQEDLNSIKALPAPEQKEEEE--EEEKMETKKDVEEVVSRQDQE---G 420

Query: 421 DLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSA 480
           DLL+L +E  +      D LALALFDG   V  + S     W+AFND+S DWET LV+SA
Sbjct: 421 DLLDLTDEAGVTAGTVGDSLALALFDG---VVGTESASGPGWEAFNDNSADWETDLVRSA 480

Query: 481 SNLSNQRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSASSMALGSAGRPA--ML 540
           + LS Q+++LGGGFD LLLDGMY+   + + +   + YG SGSASS+A GSAG PA  ML
Sbjct: 481 TRLSGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASML 540

Query: 541 ALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR 596
           ALPAPP    G    +S V+ DPFAAS+ VAPP YVQM++ME+KQ+LL+EEQ+MW QY R
Sbjct: 541 ALPAPPPTANGN--RNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNR 582

BLAST of Bhi05G000648 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 450.3 bits (1157), Expect = 2.5e-126
Identity = 291/656 (44.36%), Postives = 397/656 (60.52%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV S+ +  DLEVAIVKAT H++ PA E+++REIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL++EGDP +++EI +STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNN 180
           NMSDFRD + S+SWD+SAFVRT+A YLD+RLE  +  R+      G+    G     ++N
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKS-----GVSVNSGGNSSHHSN 180

Query: 181 NNNNNDNKNDD-----------------------DNNDED-------------------- 240
           N++      DD                       DNN                       
Sbjct: 181 NDDRYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGG 240

Query: 241 ----DNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVALYP 300
               D  K +    TP+R+MT E+IF +  HLQ+LL+RFL+ RPTG AKN+R++L+ALYP
Sbjct: 241 GGGRDEKKVV----TPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYP 300

Query: 301 IVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGI 360
           +VRESF++Y DI E++ +L+D+F ++   DCVK ++ +    KQ DEL  FY+WC+  G+
Sbjct: 301 VVRESFKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGV 360

Query: 361 ARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEITEEVKKEE--EIDM 420
           ARSSEYP+V++IT K LE ++EF++D++     + G+   + +IE    V +EE  E DM
Sbjct: 361 ARSSEYPEVQRITSKLLETLEEFVRDRA-----KRGKSPERKEIEAPPPVVEEEEPEPDM 420

Query: 421 NSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNLGEEHAMIREENA 480
           N IKALPPPE++        E + E+ +  ED++   E+ +  D             +  
Sbjct: 421 NEIKALPPPENYTPPPPPEPEPQPEKPQFTEDLVNLREDEVTAD-------------DQG 480

Query: 481 DKLALALFDGIGPVTD------SSSVKTLPWQ--AFNDDSQDWETTLVQSASNLSNQRAD 540
           +K ALALF G  P  +      SS+  T  WQ  A      DWE  LV++ SNL  Q A 
Sbjct: 481 NKFALALFAG-PPGNNGKWEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAA 540

Query: 541 LGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRP--AMLALPAPPTITE 598
           LGGGFD LLL+GMY Q  M+     +     GSASS+AL   G+    +LALPAP    E
Sbjct: 541 LGGGFDNLLLNGMYDQ-GMVRQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVE 600

BLAST of Bhi05G000648 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 445.7 bits (1145), Expect = 6.1e-125
Identity = 268/635 (42.20%), Postives = 394/635 (62.05%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNNNNNNN 185
           RD + S+SWD+SAFVRT+A YLD+RLE  +  RRG+          G     + +N ++ 
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNG--------GGSSSSHQSNGDDG 184

Query: 186 DNKNDDDNND--------EDDNSKAIVVRA------------------TPIRDMTSEQIF 245
            N++ DD           E  N   +  R+                  TP+R+MT E+IF
Sbjct: 185 YNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIF 244

Query: 246 SRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL 305
            +  HLQ+LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++
Sbjct: 245 GKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDM 304

Query: 306 NVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKD 365
              DCVK ++ +    KQ DEL  FY WC+  G+ARSSEYP+V++IT K LE ++EF++D
Sbjct: 305 EYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRD 364

Query: 366 KSALAQCQTGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENE 425
           ++  A+      + +++         EE +DMN IKALPPPE+       A E K ++ +
Sbjct: 365 RAKRAKSPE---RKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQ 424

Query: 426 IKEDVIKKDENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAF 485
           + +D++   E+ + GD             +  +K ALALF   GP  ++       W+AF
Sbjct: 425 VTDDLVNLREDDVSGD-------------DQGNKFALALF--AGPPANNGK-----WEAF 484

Query: 486 NDDSQ---------------DWETTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMS 545
           + D+                DWE  LV++ASNL +Q+A +GGG D LLL+GMY Q  +  
Sbjct: 485 SSDNNVTSAWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQ 544

Query: 546 TMAGSGYGVSGSASSMALGSAGR--PAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVA 598
            ++ S     GS+SS+AL   G+    +LALPAP    +        V  DPFAAS+ + 
Sbjct: 545 HVSTSEL-TGGSSSSVALPLPGKVNSHILALPAPDGTVQ-------KVNQDPFAASLTIP 599

BLAST of Bhi05G000648 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 445.7 bits (1145), Expect = 6.1e-125
Identity = 268/635 (42.20%), Postives = 394/635 (62.05%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNNNNNNN 185
           RD + S+SWD+SAFVRT+A YLD+RLE  +  RRG+          G     + +N ++ 
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNG--------GGSSSSHQSNGDDG 184

Query: 186 DNKNDDDNND--------EDDNSKAIVVRA------------------TPIRDMTSEQIF 245
            N++ DD           E  N   +  R+                  TP+R+MT E+IF
Sbjct: 185 YNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIF 244

Query: 246 SRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL 305
            +  HLQ+LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++
Sbjct: 245 GKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDM 304

Query: 306 NVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKD 365
              DCVK ++ +    KQ DEL  FY WC+  G+ARSSEYP+V++IT K LE ++EF++D
Sbjct: 305 EYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRD 364

Query: 366 KSALAQCQTGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENE 425
           ++  A+      + +++         EE +DMN IKALPPPE+       A E K ++ +
Sbjct: 365 RAKRAKSPE---RKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQ 424

Query: 426 IKEDVIKKDENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAF 485
           + +D++   E+ + GD             +  +K ALALF   GP  ++       W+AF
Sbjct: 425 VTDDLVNLREDDVSGD-------------DQGNKFALALF--AGPPANNGK-----WEAF 484

Query: 486 NDDSQ---------------DWETTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMS 545
           + D+                DWE  LV++ASNL +Q+A +GGG D LLL+GMY Q  +  
Sbjct: 485 SSDNNVTSAWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQ 544

Query: 546 TMAGSGYGVSGSASSMALGSAGR--PAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVA 598
            ++ S     GS+SS+AL   G+    +LALPAP    +        V  DPFAAS+ + 
Sbjct: 545 HVSTSEL-TGGSSSSVALPLPGKVNSHILALPAPDGTVQ-------KVNQDPFAASLTIP 599

BLAST of Bhi05G000648 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 7.4e-192
Identity = 374/605 (61.82%), Postives = 462/605 (76.36%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSTSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE+++REILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+ EGD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINN 180
           LNMSDFRD S+SNSWDYSAFVRT+ALYLDERL+FRMQ+R GKR  + +  E  +++Q   
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQ--- 180

Query: 181 NNNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
                        +    D S AIVVR+ PI +M +EQIF R QHLQQLL+RFLACRPTG
Sbjct: 181 -------------DQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            A+NNRVV+VALYPIV+ESFQIYYD+TEIMGILI+RFMEL++ D +KVY+IFCRV KQF+
Sbjct: 241 NARNNRVVIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFE 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIE- 360
           EL+ FYSWC+ +GIARSSEYP++EKIT KKL++MDEFI+DKSAL   +  +       E 
Sbjct: 301 ELDQFYSWCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADED 360

Query: 361 ----ITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIG 420
                TEEV +E+E DMN+IKALP P   +E+D+      + E E KE+VI + +   +G
Sbjct: 361 DDEARTEEVNEEQE-DMNAIKALPEPPPKEEDDV------KPEEEAKEEVIIEKKQEEMG 420

Query: 421 DLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLP-WQAFNDDSQDWETTLVQS 480
           DLL+LG  +     +  D LALALFD  GP    S  ++ P W+AF DDS DWET LVQ+
Sbjct: 421 DLLDLGNTNGGEAGQAGDSLALALFD--GPYASGSGSESGPGWEAFKDDSADWETALVQT 480

Query: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSASSMALGSAGRPA--M 540
           A+NLS Q+++LGGGFDMLLL+GMY+   + + +   + YG SGSASSMA GSAGRPA  M
Sbjct: 481 ATNLSGQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATM 540

Query: 541 LALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYA 596
           LALPAP T     G  +S V  DPFAAS+ VAPP YVQM++ME+KQ++L+EEQ+MW QY+
Sbjct: 541 LALPAPSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYS 580

BLAST of Bhi05G000648 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 651.4 bits (1679), Expect = 1.0e-185
Identity = 361/604 (59.77%), Postives = 464/604 (76.82%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-STSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE++++REIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+++GD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINN 180
           LNMSDFRD SQS+SWDYSAFVRT+ALYLDERL++RMQ RRGK+ + G    +G   + ++
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDD 180

Query: 181 NNNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
           +   +ND +           SKAIVV++ P+ +M +E+IF+R QHLQQLL+RFLACRPTG
Sbjct: 181 HRGTSNDIR-----------SKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            AKNNRVV+VA+YPIV+ESFQ+YY+ITEIMG+LI+RFMEL++HD +KVYEIFCRV KQFD
Sbjct: 241 NAKNNRVVIVAMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFD 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEI 360
           EL+ FY WC+ + +ARSSEYP++EKIT KKL++MDEFI+DKSALA  QT +  ++   + 
Sbjct: 301 ELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDEFIRDKSALA-AQTTKSSSKRSNKS 360

Query: 361 TEEVKKEEEI-----DMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIG 420
            EE  K E I     D+NSIKALP PE  +EE+    E  E + +++E V ++D+    G
Sbjct: 361 EEEESKTEYIQENQEDLNSIKALPAPEQKEEEE--EEEKMETKKDVEEVVSRQDQE---G 420

Query: 421 DLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSA 480
           DLL+L +E  +      D LALALFDG   V  + S     W+AFND+S DWET LV+SA
Sbjct: 421 DLLDLTDEAGVTAGTVGDSLALALFDG---VVGTESASGPGWEAFNDNSADWETDLVRSA 480

Query: 481 SNLSNQRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSASSMALGSAGRPA--ML 540
           + LS Q+++LGGGFD LLLDGMY+   + + +   + YG SGSASS+A GSAG PA  ML
Sbjct: 481 TRLSGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASML 540

Query: 541 ALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR 596
           ALPAPP    G    +S V+ DPFAAS+ VAPP YVQM++ME+KQ+LL+EEQ+MW QY R
Sbjct: 541 ALPAPPPTANGN--RNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNR 582

BLAST of Bhi05G000648 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 450.3 bits (1157), Expect = 3.5e-125
Identity = 291/656 (44.36%), Postives = 397/656 (60.52%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV S+ +  DLEVAIVKAT H++ PA E+++REIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL++EGDP +++EI +STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNN 180
           NMSDFRD + S+SWD+SAFVRT+A YLD+RLE  +  R+      G+    G     ++N
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKS-----GVSVNSGGNSSHHSN 180

Query: 181 NNNNNDNKNDD-----------------------DNNDED-------------------- 240
           N++      DD                       DNN                       
Sbjct: 181 NDDRYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGG 240

Query: 241 ----DNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVALYP 300
               D  K +    TP+R+MT E+IF +  HLQ+LL+RFL+ RPTG AKN+R++L+ALYP
Sbjct: 241 GGGRDEKKVV----TPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYP 300

Query: 301 IVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGI 360
           +VRESF++Y DI E++ +L+D+F ++   DCVK ++ +    KQ DEL  FY+WC+  G+
Sbjct: 301 VVRESFKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGV 360

Query: 361 ARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEITEEVKKEE--EIDM 420
           ARSSEYP+V++IT K LE ++EF++D++     + G+   + +IE    V +EE  E DM
Sbjct: 361 ARSSEYPEVQRITSKLLETLEEFVRDRA-----KRGKSPERKEIEAPPPVVEEEEPEPDM 420

Query: 421 NSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNLGEEHAMIREENA 480
           N IKALPPPE++        E + E+ +  ED++   E+ +  D             +  
Sbjct: 421 NEIKALPPPENYTPPPPPEPEPQPEKPQFTEDLVNLREDEVTAD-------------DQG 480

Query: 481 DKLALALFDGIGPVTD------SSSVKTLPWQ--AFNDDSQDWETTLVQSASNLSNQRAD 540
           +K ALALF G  P  +      SS+  T  WQ  A      DWE  LV++ SNL  Q A 
Sbjct: 481 NKFALALFAG-PPGNNGKWEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAA 540

Query: 541 LGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRP--AMLALPAPPTITE 598
           LGGGFD LLL+GMY Q  M+     +     GSASS+AL   G+    +LALPAP    E
Sbjct: 541 LGGGFDNLLLNGMYDQ-GMVRQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVE 600

BLAST of Bhi05G000648 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 445.7 bits (1145), Expect = 8.6e-124
Identity = 268/635 (42.20%), Postives = 394/635 (62.05%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNNNNNNN 185
           RD + S+SWD+SAFVRT+A YLD+RLE  +  RRG+          G     + +N ++ 
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNG--------GGSSSSHQSNGDDG 184

Query: 186 DNKNDDDNND--------EDDNSKAIVVRA------------------TPIRDMTSEQIF 245
            N++ DD           E  N   +  R+                  TP+R+MT E+IF
Sbjct: 185 YNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIF 244

Query: 246 SRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL 305
            +  HLQ+LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++
Sbjct: 245 GKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDM 304

Query: 306 NVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKD 365
              DCVK ++ +    KQ DEL  FY WC+  G+ARSSEYP+V++IT K LE ++EF++D
Sbjct: 305 EYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRD 364

Query: 366 KSALAQCQTGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENE 425
           ++  A+      + +++         EE +DMN IKALPPPE+       A E K ++ +
Sbjct: 365 RAKRAKSPE---RKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQ 424

Query: 426 IKEDVIKKDENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAF 485
           + +D++   E+ + GD             +  +K ALALF   GP  ++       W+AF
Sbjct: 425 VTDDLVNLREDDVSGD-------------DQGNKFALALF--AGPPANNGK-----WEAF 484

Query: 486 NDDSQ---------------DWETTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMS 545
           + D+                DWE  LV++ASNL +Q+A +GGG D LLL+GMY Q  +  
Sbjct: 485 SSDNNVTSAWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQ 544

Query: 546 TMAGSGYGVSGSASSMALGSAGR--PAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVA 598
            ++ S     GS+SS+AL   G+    +LALPAP    +        V  DPFAAS+ + 
Sbjct: 545 HVSTSEL-TGGSSSSVALPLPGKVNSHILALPAPDGTVQ-------KVNQDPFAASLTIP 599

BLAST of Bhi05G000648 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 7.0e-65
Identity = 140/346 (40.46%), Postives = 218/346 (63.01%), Query Frame = 0

Query: 3   PSKIRRALGAVKDKTSIGLAKVGSSTS----LSDLEVAIVKATRH-EEYPAEERHVREIL 62
           PSK+++A+GAVKD+TSI LAKV +  +    L+ LEVAI+KAT H EE P ++R V EIL
Sbjct: 2   PSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEIL 61

Query: 63  SLTCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGT 122
            +    +++ ++C   + RR+ +T+NW VALK+L+L+ R+  +GDP + +E+  + +RG 
Sbjct: 62  GIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGA 121

Query: 123 RFLNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQI 182
           + LN+S FRD S S  WD++AFVRTFALYLDERL+  +  +  +R          Q  +I
Sbjct: 122 KILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYT-----NREQTGRI 181

Query: 183 NNNNNNNNDNKNDDDNNDEDDNSKAIVVRATP-IRDMTSEQIFSRTQHLQQLLERFLACR 242
           + N+   +             N KA +    P +RDM    +  +  + Q+LL+R +A R
Sbjct: 182 STNSTTRS-----------RFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATR 241

Query: 243 PTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGK 302
           PTG AK NR+V ++LY +++ESF +Y DI++ + +L+D F  L    C+  ++   R  K
Sbjct: 242 PTGDAKANRLVKMSLYAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASK 301

Query: 303 QFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSA 343
           QF+EL  FY   ++IGI R+SEYP ++KI+ + LE + EF+KD+S+
Sbjct: 302 QFEELNAFYDLSKSIGIGRTSEYPSIQKISLELLETLQEFLKDQSS 331

BLAST of Bhi05G000648 vs. ExPASy TrEMBL
Match: A0A0A0K783 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G058550 PE=4 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.2e-293
Identity = 543/621 (87.44%), Postives = 577/621 (92.91%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDE-------EGQ 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLD+E       + Q
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHHQQQ 180

Query: 181 QQQINNNNNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFL 240
           QQ IN++++NNN++ ND+DNNDE+D+SKAI+VRATP+RDMTSEQIFSRTQHLQQLLERFL
Sbjct: 181 QQHINSSSSNNNEDNNDNDNNDEEDHSKAIIVRATPMRDMTSEQIFSRTQHLQQLLERFL 240

Query: 241 ACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCR 300
           ACRPTGAAKNNRVVLVALYP+VRESFQIYYDITEIMGILIDRFM+LNV DCVKVYEIFCR
Sbjct: 241 ACRPTGAAKNNRVVLVALYPVVRESFQIYYDITEIMGILIDRFMDLNVQDCVKVYEIFCR 300

Query: 301 VGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKN 360
           VGKQFDELE+FYSWCRTIGIARS+EYP+VEKITPKKLEVMDEFIKD+SALAQCQ  R K 
Sbjct: 301 VGKQFDELEMFYSWCRTIGIARSAEYPEVEKITPKKLEVMDEFIKDRSALAQCQNDREKT 360

Query: 361 QLQIEITEEVKK----EEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKD- 420
           Q Q+EITEE  K    EEE+D+NSIKALPPPED KEE+ITAIEV+EEE EIKED +KKD 
Sbjct: 361 QPQLEITEEEHKEEEEEEEVDVNSIKALPPPEDIKEEEITAIEVEEEEKEIKEDEMKKDN 420

Query: 421 ENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWET 480
           +N ++GDLLNLGEEHAMIRE NADKLA ALFDG GPV   SSVK LPWQAFNDDS DWET
Sbjct: 421 DNSMMGDLLNLGEEHAMIREANADKLATALFDGSGPV--DSSVKALPWQAFNDDSADWET 480

Query: 481 TLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRP 540
            LVQSAS+L NQ+ADLGGGFDMLLLDGMYKQT + STMAGSGYGVSGSASSMALGSAGRP
Sbjct: 481 ALVQSASHLPNQKADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRP 540

Query: 541 AMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQ 600
           AMLALPA PT  EG G SSSSVVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQ
Sbjct: 541 AMLALPA-PTTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQ 600

Query: 601 YARDGRQIQHNPYTGGFTHSY 610
           YARDGRQIQHNPYTGGFTHSY
Sbjct: 601 YARDGRQIQHNPYTGGFTHSY 618

BLAST of Bhi05G000648 vs. ExPASy TrEMBL
Match: A0A5A7SK49 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002810 PE=4 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 2.1e-290
Identity = 539/612 (88.07%), Postives = 565/612 (92.32%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNN 180
           NMSDFRD S SNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLD+E+        N
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 NNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           + ++N+N  D+DNNDE+DNSKAIV+RATPIRDMTS+QIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL + DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           LELFYSWC+TIGIARSSEYP+VEKITPKKLEVMDEFIKD+SALAQCQ  R K Q Q+EI 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EEVKK--EEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKD-ENPLIGDLL 420
           EE  K  EEE DMNS+KALPPPED KEE+ TA EVKEEE EIKED +KKD EN ++GDLL
Sbjct: 361 EEEHKVEEEEEDMNSVKALPPPEDVKEEETTATEVKEEEKEIKEDEMKKDNENSMMGDLL 420

Query: 421 NLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNL 480
           NLGEEHAMIREENADKLALALFDG GPV   SSVK LPWQAFND+S DWE+ LVQSASNL
Sbjct: 421 NLGEEHAMIREENADKLALALFDGSGPV--DSSVKALPWQAFNDESADWESALVQSASNL 480

Query: 481 SNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPP 540
           SNQRADLGGGFDMLLLDGMYKQT + STMAGSGYGVSGSASSMALGSAGRPAMLALPAPP
Sbjct: 481 SNQRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPP 540

Query: 541 TITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600
           T  EG G SSSSVVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ
Sbjct: 541 T-PEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600

Query: 601 HNPYTGGFTHSY 610
           HNPYTGGFTHSY
Sbjct: 601 HNPYTGGFTHSY 609

BLAST of Bhi05G000648 vs. ExPASy TrEMBL
Match: A0A1S3C1P9 (putative clathrin assembly protein At1g03050 OS=Cucumis melo OX=3656 GN=LOC103495455 PE=4 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 2.1e-290
Identity = 539/612 (88.07%), Postives = 565/612 (92.32%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNN 180
           NMSDFRD S SNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLD+E+        N
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 NNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           + ++N+N  D+DNNDE+DNSKAIV+RATPIRDMTS+QIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL + DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           LELFYSWC+TIGIARSSEYP+VEKITPKKLEVMDEFIKD+SALAQCQ  R K Q Q+EI 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EEVKK--EEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKD-ENPLIGDLL 420
           EE  K  EEE DMNS+KALPPPED KEE+ TA EVKEEE EIKED +KKD EN ++GDLL
Sbjct: 361 EEEHKVEEEEEDMNSVKALPPPEDVKEEETTATEVKEEEKEIKEDEMKKDNENSMMGDLL 420

Query: 421 NLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNL 480
           NLGEEHAMIREENADKLALALFDG GPV   SSVK LPWQAFND+S DWE+ LVQSASNL
Sbjct: 421 NLGEEHAMIREENADKLALALFDGSGPV--DSSVKALPWQAFNDESADWESALVQSASNL 480

Query: 481 SNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPP 540
           SNQRADLGGGFDMLLLDGMYKQT + STMAGSGYGVSGSASSMALGSAGRPAMLALPAPP
Sbjct: 481 SNQRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPP 540

Query: 541 TITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600
           T  EG G SSSSVVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ
Sbjct: 541 T-PEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600

Query: 601 HNPYTGGFTHSY 610
           HNPYTGGFTHSY
Sbjct: 601 HNPYTGGFTHSY 609

BLAST of Bhi05G000648 vs. ExPASy TrEMBL
Match: A0A6J1HJ28 (putative clathrin assembly protein At1g03050 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464026 PE=4 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 3.7e-263
Identity = 497/624 (79.65%), Postives = 544/624 (87.18%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNN 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLE+RMQSRRG+RSAFGL +EE QQQ  N N
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGLLEEEDQQQNSNRN 180

Query: 181 NNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           +N++N N +DDD+N   DN+K I++RATP+ +MT+EQIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 SNSSNTNNDDDDDN---DNNKTIMIRATPLHEMTTEQIFSKTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AK NRVVLVALYPIV+ESFQIYYDITE MGILIDRFM+L + DCV+VYEIFCRVGKQFDE
Sbjct: 241 AKVNRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           LE+FYSWCR IGIARS+EYP+VEKITPKKLEVMDEFIKDKSALAQC         ++EIT
Sbjct: 301 LEMFYSWCRNIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCLRN------EVEIT 360

Query: 361 EEVKKEE-------------EIDMNSIKALPPPEDFKEEDITAIEVKEEE--NEIKEDVI 420
           EE ++EE             E DMNSIKALPPPE F+EE  TA+ V EE   NE+K    
Sbjct: 361 EEEEEEEEEEEEDEDEEEEQEHDMNSIKALPPPESFEEEQ-TALLVNEENETNEMKAKEK 420

Query: 421 KKDENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQD 480
             +  P++GD LNL EEH M REENA+KLA ALFDG  P+ D +SV  LPW+AF+DD+ D
Sbjct: 421 SDEHYPMMGDFLNLDEEHQMTREENAEKLAFALFDGSAPIID-ASVPALPWEAFSDDAPD 480

Query: 481 WETTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSA 540
           WET LVQSASNLSNQR DLGGGFDMLLLDGMYKQTT MSTMAGSGYGVSGSASSMALGSA
Sbjct: 481 WETALVQSASNLSNQRTDLGGGFDMLLLDGMYKQTTTMSTMAGSGYGVSGSASSMALGSA 540

Query: 541 GRPAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLM 600
           GRPA+LALPAP T +EG   SSSSV+SDPFAAS+AVAPP YVQMSEMERK+KLLVEEQLM
Sbjct: 541 GRPALLALPAPST-SEGSVASSSSVMSDPFAASVAVAPPAYVQMSEMERKKKLLVEEQLM 600

Query: 601 WQQYARDGRQIQHNPYTGGFTHSY 610
           WQQYARDGRQIQ NPYTGG+THSY
Sbjct: 601 WQQYARDGRQIQPNPYTGGYTHSY 612

BLAST of Bhi05G000648 vs. ExPASy TrEMBL
Match: A0A6J1HSL3 (putative clathrin assembly protein At1g03050 OS=Cucurbita maxima OX=3661 GN=LOC111467038 PE=4 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.4e-262
Identity = 491/619 (79.32%), Postives = 545/619 (88.05%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEEGQQQQINNN 180
           NMSDFRDTS+SNSWDYSAFVRT+ALYLDERLE+RMQSRRG+RSAFGL +EE QQQ  N  
Sbjct: 121 NMSDFRDTSESNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGLQEEEDQQQNSNRK 180

Query: 181 NNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           +N++N N +DDD+N   DN+K IV+R TP+ +MT+EQIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 SNSSNTNNDDDDDN---DNNKVIVIRVTPLHEMTTEQIFSKTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AK NRVVLVALYPIV+ESFQIYYDITE MGILIDRFM+L + DCV+VYEIFCRVGKQFDE
Sbjct: 241 AKINRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           LE+FYSWCR+IGIARS+EYP+VEKITPKKLEVMDEFIKDKSALAQC    G    ++EIT
Sbjct: 301 LEIFYSWCRSIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCLRNGGD---EVEIT 360

Query: 361 EEVKKEE---------EIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDE-N 420
           EE + ++         E DMNSIKALPPPE FKEE I  +  +E +N   +   K+DE +
Sbjct: 361 EEDEDKDEDEEQEHKHEHDMNSIKALPPPESFKEEQIALLVNEENQNNEMKAKEKRDEHH 420

Query: 421 PLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTL 480
           P++GD LNL EEH M REENA++LA ALFDG  P+ D +SV  LPW+AF+DD+ DWET L
Sbjct: 421 PMMGDFLNLDEEHQMTREENAEQLAFALFDGSAPIID-ASVPALPWEAFSDDTPDWETAL 480

Query: 481 VQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAM 540
           VQSAS+LSNQR DLGGGFDMLLLDGMYKQTT M T+AGSGYGVSGSASSMALGSAGRPAM
Sbjct: 481 VQSASSLSNQRTDLGGGFDMLLLDGMYKQTTRMPTVAGSGYGVSGSASSMALGSAGRPAM 540

Query: 541 LALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYA 600
           LALPAP T +EGG VSSSSV+SDPFAAS+AVAPP YVQ+SEMERKQKLLVEE+LMWQQYA
Sbjct: 541 LALPAPST-SEGGVVSSSSVMSDPFAASVAVAPPAYVQISEMERKQKLLVEEKLMWQQYA 600

Query: 601 RDGRQIQHNPYTGGFTHSY 610
           RDGRQIQ NPYTGG+THSY
Sbjct: 601 RDGRQIQPNPYTGGYTHSY 611

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G03050.15.3e-19361.82ENTH/ANTH/VHS superfamily protein [more]
AT4G02650.17.4e-18759.77ENTH/ANTH/VHS superfamily protein [more]
AT2G25430.12.5e-12644.36epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT4G32285.16.1e-12542.20ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.26.1e-12542.20ENTH/ANTH/VHS superfamily protein [more]
Match NameE-valueIdentityDescription
Q9SA657.4e-19261.82Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX471.0e-18559.77Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LF203.5e-12544.36Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8S9J88.6e-12442.20Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9ZVN67.0e-6540.46Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0K7831.2e-29387.44ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G058550 PE=4 S... [more]
A0A5A7SK492.1e-29088.07Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C1P92.1e-29088.07putative clathrin assembly protein At1g03050 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1HJ283.7e-26379.65putative clathrin assembly protein At1g03050 isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1HSL31.4e-26279.32putative clathrin assembly protein At1g03050 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 31..161
e-value: 2.0E-47
score: 173.6
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 25..161
score: 34.806278
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 2..157
e-value: 1.4E-42
score: 146.9
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 31..154
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 32..339
e-value: 1.4E-97
score: 326.0
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 205..350
e-value: 1.6E-39
score: 136.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 164..198
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..187
NoneNo IPR availablePANTHERPTHR22951:SF66BNAC09G00100D PROTEINcoord: 1..597
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 1..597
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 33..154
e-value: 9.72248E-67
score: 211.714
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 209..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000648Bhi05M000648mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0042744 hydrogen peroxide catabolic process
biological_process GO:0006979 response to oxidative stress
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0048268 clathrin coat assembly
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0004601 peroxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0020037 heme binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005543 phospholipid binding