Bhi05G000615 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000615
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionUDP-glycosyltransferase 74E2-like
Locationchr5: 25021112 .. 25021453 (+)
RNA-Seq ExpressionBhi05G000615
SyntenyBhi05G000615
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGCCATAGAAATAATGTAGATGAGGAAGGTAGAAGAGTTCATATTGTAGTGGTTCCATCGCCAGCCCAAGGCCACATAAATCCACTTCTCCAATTCTCCAAGCACCTTGCAGCTCGTCCAGGTCTCACCGTAACATTCCCCACCGTCTTCGGCGACACCGCCGTCGTCAATCTACCGGCATCAACGGTCCCCTCACTCACCCTTCAACAAGTATCTCTATCGCCCTACAATGGAACCGAGCCGGAGACTCCCGTAGGTTTCTGGGAACGGACTCAAACGTCCATCCGTTTGCATCTCATAGAACTCATTGCTCGACACGAACGTAGCAAGGACTGA

mRNA sequence

ATGGAAAGCCATAGAAATAATGTAGATGAGGAAGGTAGAAGAGTTCATATTGTAGTGGTTCCATCGCCAGCCCAAGGCCACATAAATCCACTTCTCCAATTCTCCAAGCACCTTGCAGCTCGTCCAGGTCTCACCGTAACATTCCCCACCGTCTTCGGCGACACCGCCGTCGTCAATCTACCGGCATCAACGGTCCCCTCACTCACCCTTCAACAAGTATCTCTATCGCCCTACAATGGAACCGAGCCGGAGACTCCCGTAGGTTTCTGGGAACGGACTCAAACGTCCATCCGTTTGCATCTCATAGAACTCATTGCTCGACACGAACGTAGCAAGGACTGA

Coding sequence (CDS)

ATGGAAAGCCATAGAAATAATGTAGATGAGGAAGGTAGAAGAGTTCATATTGTAGTGGTTCCATCGCCAGCCCAAGGCCACATAAATCCACTTCTCCAATTCTCCAAGCACCTTGCAGCTCGTCCAGGTCTCACCGTAACATTCCCCACCGTCTTCGGCGACACCGCCGTCGTCAATCTACCGGCATCAACGGTCCCCTCACTCACCCTTCAACAAGTATCTCTATCGCCCTACAATGGAACCGAGCCGGAGACTCCCGTAGGTTTCTGGGAACGGACTCAAACGTCCATCCGTTTGCATCTCATAGAACTCATTGCTCGACACGAACGTAGCAAGGACTGA

Protein sequence

MESHRNNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNLPASTVPSLTLQQVSLSPYNGTEPETPVGFWERTQTSIRLHLIELIARHERSKD
Homology
BLAST of Bhi05G000615 vs. TAIR 10
Match: AT4G01070.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 3.1e-06
Identity = 28/74 (37.84%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 10 EEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTF-------PTVFGDTAVVNLPA 69
          EE +  H+ ++PSP  GH+ PL++F+K L    GLTVTF       P+    T + +LP 
Sbjct: 2  EESKTPHVAIIPSPGMGHLIPLVEFAKRLVHLHGLTVTFVIAGEGPPSKAQRTVLDSLP- 61

Query: 70 STVPSLTLQQVSLS 77
          S++ S+ L  V L+
Sbjct: 62 SSISSVFLPPVDLT 74

BLAST of Bhi05G000615 vs. TAIR 10
Match: AT4G01070.2 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 3.1e-06
Identity = 28/74 (37.84%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 10 EEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTF-------PTVFGDTAVVNLPA 69
          EE +  H+ ++PSP  GH+ PL++F+K L    GLTVTF       P+    T + +LP 
Sbjct: 2  EESKTPHVAIIPSPGMGHLIPLVEFAKRLVHLHGLTVTFVIAGEGPPSKAQRTVLDSLP- 61

Query: 70 STVPSLTLQQVSLS 77
          S++ S+ L  V L+
Sbjct: 62 SSISSVFLPPVDLT 74

BLAST of Bhi05G000615 vs. TAIR 10
Match: AT3G02100.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 4.1e-06
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 6  NNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTF-PTVFGDTAVV-NLPAS 65
          NN ++   R H+VV+P PAQGH+ PL+ FS++L A+ G+ +TF  T F    ++ +LP S
Sbjct: 3  NNSNKRMGRPHVVVIPYPAQGHVLPLISFSRYL-AKQGIQITFINTEFNHNRIISSLPNS 62

Query: 66 TVPSLTLQQVSL 76
                  Q++L
Sbjct: 63 PHEDYVGDQINL 73

BLAST of Bhi05G000615 vs. TAIR 10
Match: AT1G22340.1 (UDP-glucosyl transferase 85A7 )

HSP 1 Score: 45.1 bits (105), Expect = 4.5e-05
Identity = 25/48 (52.08%), Postives = 32/48 (66.67%), Query Frame = 0

Query: 1  MESHRNNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTF 49
          MESH   V    ++ H+V VP PAQGHINP+L+ +K L A+ G  VTF
Sbjct: 1  MESH---VVHNAQKPHVVCVPYPAQGHINPMLKVAKLLYAK-GFHVTF 44

BLAST of Bhi05G000615 vs. TAIR 10
Match: AT1G05680.1 (Uridine diphosphate glycosyltransferase 74E2 )

HSP 1 Score: 45.1 bits (105), Expect = 4.5e-05
Identity = 33/92 (35.87%), Postives = 50/92 (54.35%), Query Frame = 0

Query: 16  HIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNLPASTV-PSLTLQQVS 75
           H++V+P P QGHI P+ QF K LA++ GL +T   V    +    P  T   S+T+  +S
Sbjct: 6   HLIVLPFPGQGHITPMSQFCKRLASK-GLKLTLVLVSDKPSP---PYKTEHDSITVFPIS 65

Query: 76  LSPYNGTEPETPV-GFWERTQTSIRLHLIELI 106
                G EP   +  + ER +TSI+  L +L+
Sbjct: 66  NGFQEGEEPLQDLDDYMERVETSIKNTLPKLV 93

BLAST of Bhi05G000615 vs. ExPASy Swiss-Prot
Match: Q9M156 (UDP-glycosyltransferase 72B1 OS=Arabidopsis thaliana OX=3702 GN=UGT72B1 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 4.4e-05
Identity = 28/74 (37.84%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 10 EEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTF-------PTVFGDTAVVNLPA 69
          EE +  H+ ++PSP  GH+ PL++F+K L    GLTVTF       P+    T + +LP 
Sbjct: 2  EESKTPHVAIIPSPGMGHLIPLVEFAKRLVHLHGLTVTFVIAGEGPPSKAQRTVLDSLP- 61

Query: 70 STVPSLTLQQVSLS 77
          S++ S+ L  V L+
Sbjct: 62 SSISSVFLPPVDLT 74

BLAST of Bhi05G000615 vs. ExPASy Swiss-Prot
Match: Q9SGA8 (UDP-glycosyltransferase 83A1 OS=Arabidopsis thaliana OX=3702 GN=UGT83A1 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 5.7e-05
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 6  NNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTF-PTVFGDTAVV-NLPAS 65
          NN ++   R H+VV+P PAQGH+ PL+ FS++L A+ G+ +TF  T F    ++ +LP S
Sbjct: 3  NNSNKRMGRPHVVVIPYPAQGHVLPLISFSRYL-AKQGIQITFINTEFNHNRIISSLPNS 62

Query: 66 TVPSLTLQQVSL 76
                  Q++L
Sbjct: 63 PHEDYVGDQINL 73

BLAST of Bhi05G000615 vs. ExPASy Swiss-Prot
Match: Q6X1C0 (Crocetin glucosyltransferase 2 OS=Crocus sativus OX=82528 GN=GLT2 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 7.4e-05
Identity = 22/41 (53.66%), Postives = 28/41 (68.29%), Query Frame = 0

Query: 12 GRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVF 53
          G + HI+++P PAQGHINP+LQF K LA+   LT    T F
Sbjct: 4  GNKCHILLLPCPAQGHINPILQFGKRLASHNLLTTLVNTRF 44

BLAST of Bhi05G000615 vs. ExPASy Swiss-Prot
Match: K4CWS6 (UDP-glycosyltransferase 75C1 OS=Solanum lycopersicum OX=4081 GN=UGT75C1 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.7e-04
Identity = 26/52 (50.00%), Postives = 33/52 (63.46%), Query Frame = 0

Query: 16 HIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPT-VFGDTAVVNLPASTVP 67
          H+++V  PAQGHINP LQF+K L    G+ VTF T VF    +  + AST P
Sbjct: 5  HVLLVTFPAQGHINPSLQFAKRL-IEMGIEVTFTTSVFAHRRMAKIAASTAP 55

BLAST of Bhi05G000615 vs. ExPASy Swiss-Prot
Match: Q6VAA6 (UDP-glycosyltransferase 74G1 OS=Stevia rebaudiana OX=55670 GN=UGT74G1 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 4.8e-04
Identity = 20/59 (33.90%), Postives = 34/59 (57.63%), Query Frame = 0

Query: 16 HIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNLPASTVPSLTLQQVS 75
          H++++P P QGHINP +QF K L ++   T    T+    + +N   +T  S+ +Q +S
Sbjct: 12 HVLLIPFPLQGHINPFIQFGKRLISKGVKTTLVTTIHTLNSTLNHSNTTTTSIEIQAIS 70

BLAST of Bhi05G000615 vs. ExPASy TrEMBL
Match: A0A6J1HJ92 (UDP-glycosyltransferase 74E2-like OS=Cucurbita moschata OX=3662 GN=LOC111464072 PE=4 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 4.4e-32
Identity = 79/113 (69.91%), Postives = 88/113 (77.88%), Query Frame = 0

Query: 1   MESHRNNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNL 60
           M SH  N D+EGRRVHIVVVP+P QGHINPLLQFSKHLA R GLTVTF  VF + A ++L
Sbjct: 7   MGSH-GNEDKEGRRVHIVVVPTPTQGHINPLLQFSKHLAHR-GLTVTFLAVFTNVATIDL 66

Query: 61  PASTVPSLTLQQVSLSPYNGTEPETPVGFWERTQTSIRLHLIELIARHERSKD 114
           PA    S+TLQQVS+ PY+GT+ ETP   WER QTSIRLHLIELIARHE   D
Sbjct: 67  PA----SVTLQQVSVLPYDGTDAETPKAIWERIQTSIRLHLIELIARHENDSD 113

BLAST of Bhi05G000615 vs. ExPASy TrEMBL
Match: A0A6J1HVH9 (UDP-glycosyltransferase 74E2-like OS=Cucurbita maxima OX=3661 GN=LOC111467045 PE=4 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.8e-31
Identity = 77/113 (68.14%), Postives = 87/113 (76.99%), Query Frame = 0

Query: 1   MESHRNNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNL 60
           M SH  N D+EGRRVH+VVVP+P QGHINPLLQFSKHL A  GLTVTF  VF + A ++L
Sbjct: 1   MGSH-GNEDKEGRRVHVVVVPTPTQGHINPLLQFSKHL-AHCGLTVTFLAVFTNVAAIDL 60

Query: 61  PASTVPSLTLQQVSLSPYNGTEPETPVGFWERTQTSIRLHLIELIARHERSKD 114
           PA    S+TLQQVS+ PY+GT+ ETP   WER QTSIRLHLIELIARHE   D
Sbjct: 61  PA----SVTLQQVSVLPYDGTDAETPKAIWERIQTSIRLHLIELIARHENGSD 107

BLAST of Bhi05G000615 vs. ExPASy TrEMBL
Match: A0A6J1DTN1 (UDP-glycosyltransferase 74E2-like OS=Momordica charantia OX=3673 GN=LOC111024302 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.5e-24
Identity = 65/113 (57.52%), Postives = 75/113 (66.37%), Query Frame = 0

Query: 1   MESHRNNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNL 60
           M SH       GR VH+VVVP P QGHINPLLQF+KHL  R GL VT PT+  ++   +L
Sbjct: 1   MGSHEEG-QRRGREVHVVVVPCPTQGHINPLLQFAKHLTHR-GLNVTLPTILTNSNPTHL 60

Query: 61  PASTVPSLTLQQVSLSPYNGTEPETPVGFWERTQTSIRLHLIELIARHERSKD 114
              T  SLT+Q VSL PY GTEPE P+ FW+R Q SIRLHL EL+ R E S D
Sbjct: 61  ALHT--SLTIQHVSLLPYQGTEPENPLAFWDRRQASIRLHLTELLTRRESSGD 109

BLAST of Bhi05G000615 vs. ExPASy TrEMBL
Match: A0A5A7UV08 (Glycosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00290 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.5e-20
Identity = 56/108 (51.85%), Postives = 72/108 (66.67%), Query Frame = 0

Query: 1   MESHRNNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNL 60
           M S+     E G  VH+VV+P P QGHINPLLQF+KHL    GL VT PT+  +++ ++ 
Sbjct: 1   MGSNFEERKENGNEVHVVVIPYPTQGHINPLLQFAKHL-HHEGLKVTIPTILTNSSSLH- 60

Query: 61  PASTVPSLTLQQVSLSPYNGTEPETPVGFWERTQTSIRLHLIELIARH 109
               +P+LT+Q VSL PY GT+PET   F ER Q SIRLHL +L+ RH
Sbjct: 61  ---DLPNLTIQNVSLFPYQGTDPETLHSFSERRQASIRLHLTQLLTRH 103

BLAST of Bhi05G000615 vs. ExPASy TrEMBL
Match: A0A1S3BMV2 (Glycosyltransferase OS=Cucumis melo OX=3656 GN=LOC103491292 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.5e-20
Identity = 56/108 (51.85%), Postives = 72/108 (66.67%), Query Frame = 0

Query: 1   MESHRNNVDEEGRRVHIVVVPSPAQGHINPLLQFSKHLAARPGLTVTFPTVFGDTAVVNL 60
           M S+     E G  VH+VV+P P QGHINPLLQF+KHL    GL VT PT+  +++ ++ 
Sbjct: 1   MGSNFEERKENGNEVHVVVIPYPTQGHINPLLQFAKHL-HHEGLKVTIPTILTNSSSLH- 60

Query: 61  PASTVPSLTLQQVSLSPYNGTEPETPVGFWERTQTSIRLHLIELIARH 109
               +P+LT+Q VSL PY GT+PET   F ER Q SIRLHL +L+ RH
Sbjct: 61  ---DLPNLTIQNVSLFPYQGTDPETLHSFSERRQASIRLHLTQLLTRH 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G01070.13.1e-0637.84UDP-Glycosyltransferase superfamily protein [more]
AT4G01070.23.1e-0637.84UDP-Glycosyltransferase superfamily protein [more]
AT3G02100.14.1e-0638.89UDP-Glycosyltransferase superfamily protein [more]
AT1G22340.14.5e-0552.08UDP-glucosyl transferase 85A7 [more]
AT1G05680.14.5e-0535.87Uridine diphosphate glycosyltransferase 74E2 [more]
Match NameE-valueIdentityDescription
Q9M1564.4e-0537.84UDP-glycosyltransferase 72B1 OS=Arabidopsis thaliana OX=3702 GN=UGT72B1 PE=1 SV=... [more]
Q9SGA85.7e-0538.89UDP-glycosyltransferase 83A1 OS=Arabidopsis thaliana OX=3702 GN=UGT83A1 PE=2 SV=... [more]
Q6X1C07.4e-0553.66Crocetin glucosyltransferase 2 OS=Crocus sativus OX=82528 GN=GLT2 PE=1 SV=1[more]
K4CWS61.7e-0450.00UDP-glycosyltransferase 75C1 OS=Solanum lycopersicum OX=4081 GN=UGT75C1 PE=1 SV=... [more]
Q6VAA64.8e-0433.90UDP-glycosyltransferase 74G1 OS=Stevia rebaudiana OX=55670 GN=UGT74G1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HJ924.4e-3269.91UDP-glycosyltransferase 74E2-like OS=Cucurbita moschata OX=3662 GN=LOC111464072 ... [more]
A0A6J1HVH92.8e-3168.14UDP-glycosyltransferase 74E2-like OS=Cucurbita maxima OX=3661 GN=LOC111467045 PE... [more]
A0A6J1DTN11.5e-2457.52UDP-glycosyltransferase 74E2-like OS=Momordica charantia OX=3673 GN=LOC111024302... [more]
A0A5A7UV088.5e-2051.85Glycosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G... [more]
A0A1S3BMV28.5e-2051.85Glycosyltransferase OS=Cucumis melo OX=3656 GN=LOC103491292 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 3..113
e-value: 1.5E-17
score: 65.9
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 13..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000615Bhi05M000615mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0035251 UDP-glucosyltransferase activity