Bhi05G000577 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000577
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Very-long-chain 3-oxoacyl-CoA synthase
Location	chr5: 23431775 .. 23432596 (+)
RNA-Seq Expression	Bhi05G000577
Synteny	Bhi05G000577

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCTAAATATCATCGATAGCATCTATTACTGGCTTGTAAACCACCCCAAAATCGTCAATTTCTCATGGTCTCAAGGCGAAACCTTAGGCTCTTCCCCACTTTTCCTCACTCTCACAGTTCTCTCTTACCTCTCCCTTACCTTCCTCCTCTCCAACCTCTCTCTCCCTCTCCTCCCTCCCACCATTCTCAAACGAATCTCCGCCGTTCATAACCTTATCCTCCTCGCTCTCTCCTTCACAATGGCCCTTGGATGCACTCTCTCCACTTTCTCCCACGCGCCTCACCTCCACTACATTCTTTGCCTTCCACCCAAAACTCCTCCACGTGGGCCCCTTTTCTTTTGGGCTTACATATTCTACCTCTCGAAGATCGTGGAGTTCATCGACACGTTCTTGATCATCTTAAGCGGGTCGATCAAACGACTGACGTTCCTCCATGTGTACCACCACGCAACGGTGGTGATTATGTGTTACTTATGGCTTCATTCCTCACAGTCGTTGTTCCCGTTGGTTTTAGTAACGAACTCCTCGGTTCATGTGCTCATGTACACGTATTACTTGTCGTGTGCACTTGGGGTTCGGCCACGGTGGAAGAGGATTGTGACGGAGTGTCAAATTGTGCAGTTTCAATTTAGCTTTGTGGTGTTGGCTTTGATGCTATACTTTCACGTCACACATAAGGGGCCGGGATGTGCTGGTGTTTGGGGATGGTGCTTTAATGTCGTATTTTACTCCTCACTTCTGGCCTTGTTCTCGGATTTTCATGCTAAAAACTACGGCACAAATGGAAAGATGACGGTCCCCAAAAAGGTGGCGTGA

mRNA sequence

ATGAATCTAAATATCATCGATAGCATCTATTACTGGCTTGTAAACCACCCCAAAATCGTCAATTTCTCATGGTCTCAAGGCGAAACCTTAGGCTCTTCCCCACTTTTCCTCACTCTCACAGTTCTCTCTTACCTCTCCCTTACCTTCCTCCTCTCCAACCTCTCTCTCCCTCTCCTCCCTCCCACCATTCTCAAACGAATCTCCGCCGTTCATAACCTTATCCTCCTCGCTCTCTCCTTCACAATGGCCCTTGGATGCACTCTCTCCACTTTCTCCCACGCGCCTCACCTCCACTACATTCTTTGCCTTCCACCCAAAACTCCTCCACGTGGGCCCCTTTTCTTTTGGGCTTACATATTCTACCTCTCGAAGATCGTGGAGTTCATCGACACGTTCTTGATCATCTTAAGCGGGTCGATCAAACGACTGACGTTCCTCCATGTGTACCACCACGCAACGGTGGTGATTATGTGTTACTTATGGCTTCATTCCTCACAGTCGTTGTTCCCGTTGGTTTTAGTAACGAACTCCTCGGTTCATGTGCTCATGTACACGTATTACTTGTCGTGTGCACTTGGGGTTCGGCCACGGTGGAAGAGGATTGTGACGGAGTGTCAAATTGTGCAGTTTCAATTTAGCTTTGTGGTGTTGGCTTTGATGCTATACTTTCACGTCACACATAAGGGGCCGGGATGTGCTGGTGTTTGGGGATGGTGCTTTAATGTCGTATTTTACTCCTCACTTCTGGCCTTGTTCTCGGATTTTCATGCTAAAAACTACGGCACAAATGGAAAGATGACGGTCCCCAAAAAGGTGGCGTGA

Coding sequence (CDS)

ATGAATCTAAATATCATCGATAGCATCTATTACTGGCTTGTAAACCACCCCAAAATCGTCAATTTCTCATGGTCTCAAGGCGAAACCTTAGGCTCTTCCCCACTTTTCCTCACTCTCACAGTTCTCTCTTACCTCTCCCTTACCTTCCTCCTCTCCAACCTCTCTCTCCCTCTCCTCCCTCCCACCATTCTCAAACGAATCTCCGCCGTTCATAACCTTATCCTCCTCGCTCTCTCCTTCACAATGGCCCTTGGATGCACTCTCTCCACTTTCTCCCACGCGCCTCACCTCCACTACATTCTTTGCCTTCCACCCAAAACTCCTCCACGTGGGCCCCTTTTCTTTTGGGCTTACATATTCTACCTCTCGAAGATCGTGGAGTTCATCGACACGTTCTTGATCATCTTAAGCGGGTCGATCAAACGACTGACGTTCCTCCATGTGTACCACCACGCAACGGTGGTGATTATGTGTTACTTATGGCTTCATTCCTCACAGTCGTTGTTCCCGTTGGTTTTAGTAACGAACTCCTCGGTTCATGTGCTCATGTACACGTATTACTTGTCGTGTGCACTTGGGGTTCGGCCACGGTGGAAGAGGATTGTGACGGAGTGTCAAATTGTGCAGTTTCAATTTAGCTTTGTGGTGTTGGCTTTGATGCTATACTTTCACGTCACACATAAGGGGCCGGGATGTGCTGGTGTTTGGGGATGGTGCTTTAATGTCGTATTTTACTCCTCACTTCTGGCCTTGTTCTCGGATTTTCATGCTAAAAACTACGGCACAAATGGAAAGATGACGGTCCCCAAAAAGGTGGCGTGA

Protein sequence

MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLPPTILKRISAVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPPKTPPRGPLFFWAYIFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVHVLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGCAGVWGWCFNVVFYSSLLALFSDFHAKNYGTNGKMTVPKKVA

Homology

[Close]

BLAST of Bhi05G000577 vs. TAIR 10
Match: AT3G06470.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 309.3 bits (791), Expect = 3.1e-84
Identity = 163/265 (61.51%), Postives = 198/265 (74.72%), Query Frame = 0


Query: 4   NIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNL--SLPLLPP 63
           +I  S+ YWLVNHP I NF+W +GETLGS+  F+++ V  YLS TFLL +   SLP L P
Sbjct: 3   SIYSSLTYWLVNHPYISNFTWIEGETLGSTVFFVSVVVSVYLSATFLLRSAIDSLPSLSP 62

Query: 64  TILKRISAVHNLILLALSFTMALGCTLS-TFSHA---PHLHYI--LCLPPKTPPRGPLFF 123
            ILK I+AVH+LIL  LS  MA+GCTLS T SHA   P   ++  +C P    P GPLFF
Sbjct: 63  RILKPITAVHSLILCLLSLVMAVGCTLSITSSHASSDPMARFLHAICFPVDVKPNGPLFF 122

Query: 124 WAYIFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVT 183
           WA +FYLSKI+EF DT LIIL  SI+RL+FLHVYHHATVV+MCYLWL + QS+FP+ LVT
Sbjct: 123 WAQVFYLSKILEFGDTILIILGKSIQRLSFLHVYHHATVVVMCYLWLRTRQSMFPIALVT 182

Query: 184 NSSVHVLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGCAGV 243
           NS+VHV+MY YY  CA+G RP+WKR+VT+CQIVQF FSF +   ML  H+   G GC G+
Sbjct: 183 NSTVHVIMYGYYFLCAVGSRPKWKRLVTDCQIVQFVFSFGLSGWMLREHLF--GSGCTGI 242

Query: 244 WGWCFNVVFYSSLLALFSDFHAKNY 261
           WGWCFN  F +SLLALFS+FH+KNY
Sbjct: 243 WGWCFNAAFNASLLALFSNFHSKNY 265

[Close]

BLAST of Bhi05G000577 vs. TAIR 10
Match: AT3G06460.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 279.3 bits (713), Expect = 3.4e-75
Identity = 151/263 (57.41%), Postives = 191/263 (72.62%), Query Frame = 0


Query: 4   NIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNL--SLPLLPP 63
           ++  ++ YWLV+HP I NF+W++GETLGS+  F+ + V  YLS TFLL     SLP L P
Sbjct: 3   SVYSTLTYWLVHHPYIANFTWTEGETLGSTVFFVFVVVSLYLSATFLLRYTVDSLPTLGP 62

Query: 64  TILKRISAVHNLILLALSFTMALGCTLSTFSHA---PHLHYILCLPPKTPPRGPLFFWAY 123
            ILK I+AVH+LIL  LS TMA+GCTLS  S +     L   +C P    P+GPLFFWA 
Sbjct: 63  RILKPITAVHSLILFLLSLTMAVGCTLSLISSSDPKARLFDAVCFPLDVKPKGPLFFWAQ 122

Query: 124 IFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSS 183
           +FYLSKI+EF+DT LIIL+ SI+RL+FLHVYHHATVVI+CYLWL + QS+FP+ LV NS+
Sbjct: 123 VFYLSKILEFVDTLLIILNKSIQRLSFLHVYHHATVVILCYLWLRTRQSMFPVGLVLNST 182

Query: 184 VHVLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSF-VVLALMLYFHVTHKGPGCAGVWG 243
           VHV+MY YY  CA+G RP+WK++VT  Q+VQF F   +  A ML  H  + G GCAG+W 
Sbjct: 183 VHVIMYGYYFLCAIGSRPKWKKLVTNFQMVQFAFGMGLGAAWMLPEH--YFGSGCAGIWT 242

Query: 244 WCFNVVFYSSLLALFSDFHAKNY 261
             FN VF +SLLALF +FH+KNY
Sbjct: 243 VYFNGVFTASLLALFYNFHSKNY 263

[Close]

BLAST of Bhi05G000577 vs. TAIR 10
Match: AT4G36830.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 105.5 bits (262), Expect = 6.7e-23
Identity = 86/281 (30.60%), Postives = 138/281 (49.11%), Query Frame = 0


Query: 1   MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYL----SLTFLL----- 60
           M+  +I+SI Y+L  HP IV F WS  ++ GS+  FL  ++  Y+    SL  LL     
Sbjct: 1   MSTALINSITYFLSEHPYIVGFRWSNSQSWGSTWSFLFTSISLYIAVSSSLHILLSAVRR 60

Query: 61  SNLSLPLLPPTILKRISAVHNLILLALSFTMALGCTLSTFSH-------------APHLH 120
           SN S+P      L  I  +H+L++  LS T+  G  LS  +              A  L 
Sbjct: 61  SNRSVP------LGHIPEIHSLLMSILSATIFAGILLSAAAEIRDTRWLWRRSKTATPLQ 120

Query: 121 YILCLPPKTPPRGPLFFWAYIFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMC 180
           ++LC P  T P G +FFW+Y+FYL++ +    T   +L    +RL    ++ ++ +    
Sbjct: 121 WLLCFPLGTRPSGRVFFWSYVFYLTRFLHMFRTIFAVLRS--RRLAVSQLFCNSVMAFTS 180

Query: 181 YLWLHSSQSLFPLVLVTNSSVHVLMYTYYLSCALGV-RPRWKRIVTECQIVQFQFSFVVL 240
           +LWL  SQS   L +++ + V+ ++Y Y      G+    +   V  CQ+V    + V  
Sbjct: 181 FLWLEFSQSYQILAILSTTLVYSVVYGYRFWTGFGLPGSAFPSFVVNCQLVLVGCNLVSH 240

Query: 241 ALMLYFHVTHKGPGCAGVWGWCFNVVFYSSLLALFSDFHAK 259
           A +L  H+  KG GC G+  W  N V   ++L LF +F+ +
Sbjct: 241 AGVLTMHL-FKG-GCNGIGAWGLNSVLNGAILLLFLNFYVR 271

[Close]

BLAST of Bhi05G000577 vs. TAIR 10
Match: AT1G75000.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 97.4 bits (241), Expect = 1.8e-20
Identity = 74/266 (27.82%), Postives = 124/266 (46.62%), Query Frame = 0


Query: 11  YWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLPPTILKR---- 70
           Y+L  HP IVNF WS  ++  S+  FL   V SY+     L  L L  L  +  +R    
Sbjct: 6   YYLAEHPTIVNFRWSPTQSYASTWSFLFTAVSSYIIAAVTLHLLLLITLSLSNRRRGFSL 65

Query: 71  --ISAVHNLILLALSFTMALGCTLSTF------------SHAPHLHYILCLPPKTPPRGP 130
             I A+H+L +  +S  + +G  LS              +    L + LC P  T   G 
Sbjct: 66  GPIPALHSLTISIISAVIFVGILLSAAAEIRDTRWLWRRTRTTALQWFLCFPVGTRASGR 125

Query: 131 LFFWAYIFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLV 190
           +FFW+Y FYLS+ +    TF  ++    ++L+F  + + ++++ + +LWL  SQS   + 
Sbjct: 126 VFFWSYAFYLSRFLHLFRTFFSVIRR--RKLSFFQLINQSSLLCISFLWLEYSQSFQVVA 185

Query: 191 LVTNSSVHVLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGC 250
           ++  +  + ++Y Y     +G+R      V  CQ +      V    +L  H+  +G GC
Sbjct: 186 ILLTTVSYAVVYGYRFWTEIGLRGACFPFVGNCQAILLGCMTVCHVGVLCIHLVKRG-GC 245

Query: 251 AGVWGWCFNVVFYSSLLALFSDFHAK 259
            G+  W FN V  + +  L+  F+ K
Sbjct: 246 NGIGAWLFNSVLNAVITLLYLKFYCK 268

[Close]

BLAST of Bhi05G000577 vs. ExPASy Swiss-Prot
Match: Q9SYY4 (Elongation of fatty acids protein 3-like OS=Arabidopsis thaliana OX=3702 GN=HOS3 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 9.5e-22
Identity = 86/281 (30.60%), Postives = 138/281 (49.11%), Query Frame = 0


Query: 1   MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYL----SLTFLL----- 60
           M+  +I+SI Y+L  HP IV F WS  ++ GS+  FL  ++  Y+    SL  LL     
Sbjct: 1   MSTALINSITYFLSEHPYIVGFRWSNSQSWGSTWSFLFTSISLYIAVSSSLHILLSAVRR 60

Query: 61  SNLSLPLLPPTILKRISAVHNLILLALSFTMALGCTLSTFSH-------------APHLH 120
           SN S+P      L  I  +H+L++  LS T+  G  LS  +              A  L 
Sbjct: 61  SNRSVP------LGHIPEIHSLLMSILSATIFAGILLSAAAEIRDTRWLWRRSKTATPLQ 120

Query: 121 YILCLPPKTPPRGPLFFWAYIFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMC 180
           ++LC P  T P G +FFW+Y+FYL++ +    T   +L    +RL    ++ ++ +    
Sbjct: 121 WLLCFPLGTRPSGRVFFWSYVFYLTRFLHMFRTIFAVLRS--RRLAVSQLFCNSVMAFTS 180

Query: 181 YLWLHSSQSLFPLVLVTNSSVHVLMYTYYLSCALGV-RPRWKRIVTECQIVQFQFSFVVL 240
           +LWL  SQS   L +++ + V+ ++Y Y      G+    +   V  CQ+V    + V  
Sbjct: 181 FLWLEFSQSYQILAILSTTLVYSVVYGYRFWTGFGLPGSAFPSFVVNCQLVLVGCNLVSH 240

Query: 241 ALMLYFHVTHKGPGCAGVWGWCFNVVFYSSLLALFSDFHAK 259
           A +L  H+  KG GC G+  W  N V   ++L LF +F+ +
Sbjct: 241 AGVLTMHL-FKG-GCNGIGAWGLNSVLNGAILLLFLNFYVR 271

[Close]

BLAST of Bhi05G000577 vs. ExPASy Swiss-Prot
Match: Q86JM5 (Putative elongation of fatty acids protein DDB_G0272012 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0272012 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.2e-21
Identity = 83/266 (31.20%), Postives = 123/266 (46.24%), Query Frame = 0


Query: 20  VNFSWSQGETLGSS----PLFLTLTVLSYLSLTFLLSNLSLPLLPPTILKRISAVHNLIL 79
           VNF +  G T  S     P  ++L ++   S+ FL+ N       P  LK +S +HN IL
Sbjct: 37  VNFKFKFGTTPFSQFQILPSVISLYLVIIFSIKFLMRNRK-----PFSLKYVSILHNAIL 96

Query: 80  LALSFTMALGCTLSTFSH--APHLHYILCLPPKTPPRGPLFFWAYIFYLSKIVEFIDTFL 139
              S  M +G          A    + +C       +GP ++W+YIFY+SK  E +DT +
Sbjct: 97  CIWSLVMCVGILYEVIKRITAEGPLFTVCETVSGFDKGPAYYWSYIFYISKFYELLDTVI 156

Query: 140 IILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVHVLMYTYYLSCALG 199
           I+L    K L FLHVYHH  VV +C+ +++S  +L   V+  N+ VHV MY +Y     G
Sbjct: 157 IVLKK--KPLIFLHVYHHCIVVWLCWYFMYSGWNLQLWVVFLNTFVHVFMYYFYFQTGRG 216

Query: 200 VRPRWKRIVTECQIVQFQFSFVVLALMLYFHVT----HKGPGCAGVWGWCFNVVFYSSLL 259
               WK+ +T  QI+Q    F+ L +    H      +  P       +    +   S L
Sbjct: 217 KTVWWKKYITMIQIIQ----FICLGIAGLLHSAAINLNSSPCFTHYPAFISAYLINFSFL 276

Query: 260 ALFSDFHAKNYG----TNGKMTVPKK 272
            LFS F  K+Y     ++   T P K
Sbjct: 277 FLFSQFFVKSYSNKPTSSSSTTTPTK 291

[Close]

BLAST of Bhi05G000577 vs. ExPASy Swiss-Prot
Match: Q54TC9 (Elongation of fatty acids protein sre1 OS=Dictyostelium discoideum OX=44689 GN=sre1 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.8e-20
Identity = 81/250 (32.40%), Postives = 123/250 (49.20%), Query Frame = 0


Query: 21  NFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLPPTILKRISAVHNLILLALSF 80
           NF W  G T  SS +F   T + Y+ + F L  + +      +LK  S +HN+ L+ LSF
Sbjct: 17  NFRWESGVTPLSSYVFPFSTSVIYVLVIFALQAI-MKNKKGMVLKGFSIIHNINLIILSF 76

Query: 81  TMALGCTLSTFSH--APHLHYILCLPPKTPPRGPLFFWAYIFYLSKIVEFIDTFLIILSG 140
           +M  G   + +          ++C       +G + FW YIFYLSK  E +DT  +IL+ 
Sbjct: 77  SMMSGVMYAAYQQYLEQGAFSLVCEQSSQSVQGRIGFWIYIFYLSKYYELVDT--VILAL 136

Query: 141 SIKRLTFLHVYHHATVVIMCYLWLHSSQSLFP-LVLVTNSSVHVLMYTYYLSCALGVRPR 200
             K + FLH++HH  +V + + WLH    +      + NS +HVLMY YYL   LG  P 
Sbjct: 137 KKKPIIFLHIFHHMAMVPVTWQWLHDQWLVGSWWCTLVNSFIHVLMYYYYLQTTLG-NPC 196

Query: 201 W-KRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGC-AGVWGWCFNVVFYSSLLALFSDF 260
           W K+ +T+ QIVQF       A++ Y+ V      C A +     +    S  + LF  F
Sbjct: 197 WFKKYITKAQIVQF---LTGTAMVSYWFVIRDSEKCQAPLSPAIVSNTINSFFIILFGKF 256

Query: 261 HAKNYGTNGK 266
           +  +Y +N +
Sbjct: 257 YYDSYKSNSR 259

[Close]

BLAST of Bhi05G000577 vs. ExPASy Swiss-Prot
Match: P40319 (Elongation of fatty acids protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ELO3 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.0e-19
Identity = 72/226 (31.86%), Postives = 105/226 (46.46%), Query Frame = 0


Query: 61  PTILKRISAVHNLILLALSFTMALGCTLSTFSHAPH--LHYILCLPPKTPPRGPLFFWAY 120
           P   K +  +HNL L ++S  + L           H  L + +C      P+  L    Y
Sbjct: 101 PLKFKLLFEIHNLFLTSISLVLWLLMLEQLVPMVYHNGLFWSICSKEAFAPK--LVTLYY 160

Query: 121 IFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSS 180
           + YL+K VE IDT  ++L    K+L FLH YHH    ++CY  L    S+  +V++ N  
Sbjct: 161 LNYLTKFVELIDTVFLVLRR--KKLLFLHTYHHGATALLCYTQLIGRTSVEWVVILLNLG 220

Query: 181 VHVLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHK---------- 240
           VHV+MY YY   + G+R  WK+ VT  QI+QF    V +    Y    HK          
Sbjct: 221 VHVIMYWYYFLSSCGIRVWWKQWVTRFQIIQFLIDLVFVYFATYTFYAHKYLDGILPNKG 280

Query: 241 ---GPGCAGVWGWCFNVVFYSSLLALFSDFHAKNYGTNGKMTVPKK 272
              G   A  +G+    +  +S L LF  F+ ++Y   GK TV K+
Sbjct: 281 TCYGTQAAAAYGY----LILTSYLLLFISFYIQSYKKGGKKTVKKE 318

[Close]

BLAST of Bhi05G000577 vs. ExPASy Swiss-Prot
Match: P25358 (Elongation of fatty acids protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ELO2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.4e-19
Identity = 75/218 (34.40%), Postives = 104/218 (47.71%), Query Frame = 0


Query: 61  PTILKRISAVHNLILLALSFTMALGCT--LSTFSHAPHLHYILC-LPPKTPPRGPLFFWA 120
           P  L  +  +HNL+L +LS T+ L     L        L++ +C +   T P   L+   
Sbjct: 94  PFKLNGLFQLHNLVLTSLSLTLLLLMVEQLVPIIVQHGLYFAICNIGAWTQPLVTLY--- 153

Query: 121 YIFYLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNS 180
           Y+ Y+ K +EFIDTF ++L    K+LTFLH YHH    ++CY  L  + S+  + +  N 
Sbjct: 154 YMNYIVKFIEFIDTFFLVLKH--KKLTFLHTYHHGATALLCYTQLMGTTSISWVPISLNL 213

Query: 181 SVHVLMYTYYLSCALGVRPRWKRIVTECQIVQF-------QFSFVVLALMLYFHVTHKGP 240
            VHV+MY YY   A G+R  WK  VT  QI+QF        F+    A+ LYF +     
Sbjct: 214 GVHVVMYWYYFLAARGIRVWWKEWVTRFQIIQFVLDIGFIYFAVYQKAVHLYFPILPHCG 273

Query: 241 GCAGVWGWCF-NVVFYSSLLALFSDFHAKNYGTNGKMT 268
            C G     F      SS L LF  F+   Y   G  T
Sbjct: 274 DCVGSTTATFAGCAIISSYLVLFISFYINVYKRKGTKT 306

[Close]

BLAST of Bhi05G000577 vs. ExPASy TrEMBL
Match: A0A5D3CNK0 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00120 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.6e-144
Identity = 257/273 (94.14%), Postives = 264/273 (96.70%), Query Frame = 0


Query: 1   MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLP 60
           M+L I DS+YYWLVNHPKI+NFSWSQGETLGSSPLFLT+TV+SYLSLTFLLSNLSLP LP
Sbjct: 1   MSLTITDSLYYWLVNHPKILNFSWSQGETLGSSPLFLTVTVISYLSLTFLLSNLSLPPLP 60

Query: 61  PTILKRISAVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPPKTPPRGPLFFWAYIF 120
           PTILKRIS VHNLILLALSFTMALGCTLSTFSHAPHLHYILCLP KTPPRGPLFFWAYIF
Sbjct: 61  PTILKRISVVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPLKTPPRGPLFFWAYIF 120

Query: 121 YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180
           YLSKIVEFIDTFLIILSGS KRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH
Sbjct: 121 YLSKIVEFIDTFLIILSGSTKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180

Query: 181 VLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGCAGVWGWCF 240
           VLMYTYYLSCALG+RPRWKR+VTECQIVQFQFSFVVLALMLYFHVTHKG GCAGVWGWCF
Sbjct: 181 VLMYTYYLSCALGIRPRWKRLVTECQIVQFQFSFVVLALMLYFHVTHKGTGCAGVWGWCF 240

Query: 241 NVVFYSSLLALFSDFHAKNYGTNGKMTVPKKVA 274
           NVVFYSSLLALFSDFHAKNYG NGKMT PKKVA
Sbjct: 241 NVVFYSSLLALFSDFHAKNYGANGKMTAPKKVA 273

[Close]

BLAST of Bhi05G000577 vs. ExPASy TrEMBL
Match: A0A1S3BMM0 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103491306 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.6e-144
Identity = 257/273 (94.14%), Postives = 264/273 (96.70%), Query Frame = 0


Query: 1   MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLP 60
           M+L I DS+YYWLVNHPKI+NFSWSQGETLGSSPLFLT+TV+SYLSLTFLLSNLSLP LP
Sbjct: 1   MSLTITDSLYYWLVNHPKILNFSWSQGETLGSSPLFLTVTVISYLSLTFLLSNLSLPPLP 60

Query: 61  PTILKRISAVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPPKTPPRGPLFFWAYIF 120
           PTILKRIS VHNLILLALSFTMALGCTLSTFSHAPHLHYILCLP KTPPRGPLFFWAYIF
Sbjct: 61  PTILKRISVVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPLKTPPRGPLFFWAYIF 120

Query: 121 YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180
           YLSKIVEFIDTFLIILSGS KRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH
Sbjct: 121 YLSKIVEFIDTFLIILSGSTKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180

Query: 181 VLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGCAGVWGWCF 240
           VLMYTYYLSCALG+RPRWKR+VTECQIVQFQFSFVVLALMLYFHVTHKG GCAGVWGWCF
Sbjct: 181 VLMYTYYLSCALGIRPRWKRLVTECQIVQFQFSFVVLALMLYFHVTHKGTGCAGVWGWCF 240

Query: 241 NVVFYSSLLALFSDFHAKNYGTNGKMTVPKKVA 274
           NVVFYSSLLALFSDFHAKNYG NGKMT PKKVA
Sbjct: 241 NVVFYSSLLALFSDFHAKNYGANGKMTAPKKVA 273

[Close]

BLAST of Bhi05G000577 vs. ExPASy TrEMBL
Match: A0A0A0KHS0 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_6G411310 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.3e-143
Identity = 255/273 (93.41%), Postives = 264/273 (96.70%), Query Frame = 0


Query: 1   MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLP 60
           M+L I D +YYWLVNHPKI+NFSWSQGETLGSSPLFLT+TV++YLSLTFLLSNLSLPLLP
Sbjct: 1   MSLTITDGLYYWLVNHPKILNFSWSQGETLGSSPLFLTVTVIAYLSLTFLLSNLSLPLLP 60

Query: 61  PTILKRISAVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPPKTPPRGPLFFWAYIF 120
           PTILKRIS VHNLILLALSFTMALGCTLSTFSHAPHLHYILCLP KTPPRGPLFFWAYIF
Sbjct: 61  PTILKRISVVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPLKTPPRGPLFFWAYIF 120

Query: 121 YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180
           YLSKI+EFIDTFLIILSGS KRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH
Sbjct: 121 YLSKILEFIDTFLIILSGSTKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180

Query: 181 VLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGCAGVWGWCF 240
           VLMYTYYLSCALG+RPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKG GCAGV+GWCF
Sbjct: 181 VLMYTYYLSCALGIRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGSGCAGVYGWCF 240

Query: 241 NVVFYSSLLALFSDFHAKNYGTNGKMTVPKKVA 274
           NVVFYSSLLALFSDFHAKNYG NGKMT PKKVA
Sbjct: 241 NVVFYSSLLALFSDFHAKNYGANGKMTAPKKVA 273

[Close]

BLAST of Bhi05G000577 vs. ExPASy TrEMBL
Match: A0A6J1HGE1 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC111463845 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.6e-136
Identity = 242/273 (88.64%), Postives = 260/273 (95.24%), Query Frame = 0


Query: 1   MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLP 60
           M++ I D+++YWLVNHP I++FSWSQGETLGSSPLFLT+TV+SYLSLTFLLSNL+LP L 
Sbjct: 1   MSMQISDTLHYWLVNHPNILHFSWSQGETLGSSPLFLTITVVSYLSLTFLLSNLTLPHLR 60

Query: 61  PTILKRISAVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPPKTPPRGPLFFWAYIF 120
           P +LKRI+ VHNL+LL LSFTMALGCTL+TFSHAPHLHYILCLPPKTP +GPLFFWAY+F
Sbjct: 61  PAVLKRITVVHNLVLLVLSFTMALGCTLATFSHAPHLHYILCLPPKTPAQGPLFFWAYVF 120

Query: 121 YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180
           YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH
Sbjct: 121 YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180

Query: 181 VLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGCAGVWGWCF 240
           VLMYTYYLSCALGVRPRWKR+VTECQIVQFQFSFVVLALMLYFHVTHKG GCAGVWGWCF
Sbjct: 181 VLMYTYYLSCALGVRPRWKRLVTECQIVQFQFSFVVLALMLYFHVTHKGSGCAGVWGWCF 240

Query: 241 NVVFYSSLLALFSDFHAKNYGTNGKMTVPKKVA 274
           NVVFYSSLLALFSDFHAKNYG NGK T PKK+A
Sbjct: 241 NVVFYSSLLALFSDFHAKNYGGNGK-TAPKKLA 272

[Close]

BLAST of Bhi05G000577 vs. ExPASy TrEMBL
Match: A0A6J1HRB0 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111467063 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 1.7e-135
Identity = 241/273 (88.28%), Postives = 260/273 (95.24%), Query Frame = 0


Query: 1   MNLNIIDSIYYWLVNHPKIVNFSWSQGETLGSSPLFLTLTVLSYLSLTFLLSNLSLPLLP 60
           M++ I D+++YWLVNHP I++FSWSQGETLGSSPLFLT+TV+SYLSLTFLLSNL+LP L 
Sbjct: 1   MSMQISDTLHYWLVNHPNILHFSWSQGETLGSSPLFLTITVVSYLSLTFLLSNLTLPHLR 60

Query: 61  PTILKRISAVHNLILLALSFTMALGCTLSTFSHAPHLHYILCLPPKTPPRGPLFFWAYIF 120
           P +LK+I+ VHNL+LLALSFTMALGCTL+TFSHAPHLHYILCLP KTP +GPLFFWAY+F
Sbjct: 61  PAVLKQITIVHNLVLLALSFTMALGCTLATFSHAPHLHYILCLPQKTPAQGPLFFWAYVF 120

Query: 121 YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180
           YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH
Sbjct: 121 YLSKIVEFIDTFLIILSGSIKRLTFLHVYHHATVVIMCYLWLHSSQSLFPLVLVTNSSVH 180

Query: 181 VLMYTYYLSCALGVRPRWKRIVTECQIVQFQFSFVVLALMLYFHVTHKGPGCAGVWGWCF 240
           VLMYTYYLSCALGVRPRWKR+VTECQIVQFQFSFVVLALMLYFHVTHKG GCAGVWGWCF
Sbjct: 181 VLMYTYYLSCALGVRPRWKRLVTECQIVQFQFSFVVLALMLYFHVTHKGSGCAGVWGWCF 240

Query: 241 NVVFYSSLLALFSDFHAKNYGTNGKMTVPKKVA 274
           NVVFYSSLLALFSDFHAKNYG NGK T PKK+A
Sbjct: 241 NVVFYSSLLALFSDFHAKNYGGNGK-TAPKKLA 272

The following BLAST results are available for this feature:

BLAST of Bhi05G000577 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06470.1	3.1e-84	61.51	GNS1/SUR4 membrane protein family	[more]
AT3G06460.1	3.4e-75	57.41	GNS1/SUR4 membrane protein family	[more]
AT4G36830.1	6.7e-23	30.60	GNS1/SUR4 membrane protein family	[more]
AT1G75000.1	1.8e-20	27.82	GNS1/SUR4 membrane protein family	[more]

BLAST of Bhi05G000577 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYY4	9.5e-22	30.60	Elongation of fatty acids protein 3-like OS=Arabidopsis thaliana OX=3702 GN=HOS3...	[more]
Q86JM5	6.2e-21	31.20	Putative elongation of fatty acids protein DDB_G0272012 OS=Dictyostelium discoid...	[more]
Q54TC9	6.8e-20	32.40	Elongation of fatty acids protein sre1 OS=Dictyostelium discoideum OX=44689 GN=s...	[more]
P40319	2.0e-19	31.86	Elongation of fatty acids protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204...	[more]
P25358	3.4e-19	34.40	Elongation of fatty acids protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204...	[more]

BLAST of Bhi05G000577 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CNK0	1.6e-144	94.14	Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BMM0	1.6e-144	94.14	Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103491306 P...	[more]
A0A0A0KHS0	2.3e-143	93.41	Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_6G41131...	[more]
A0A6J1HGE1	1.6e-136	88.64	Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1HRB0	1.7e-135	88.28	Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC1114670...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002076	ELO family	PFAM	PF01151	ELO	coord: 32..263 e-value: 5.3E-51 score: 173.6
IPR002076	ELO family	PANTHER	PTHR11157	FATTY ACID ACYL TRANSFERASE-RELATED	coord: 6..266
None	No IPR available	PANTHER	PTHR11157:SF132	ELONGATION OF FATTY ACIDS PROTEIN 3-LIKE	coord: 6..266
IPR030457	ELO family, conserved site	PROSITE	PS01188	ELO	coord: 143..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000577	Bhi05M000577	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034625	fatty acid elongation, monounsaturated fatty acid
biological_process	GO:0034626	fatty acid elongation, polyunsaturated fatty acid
biological_process	GO:0019367	fatty acid elongation, saturated fatty acid
biological_process	GO:0030148	sphingolipid biosynthetic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0030176	integral component of endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009922	fatty acid elongase activity
molecular_function	GO:0102756	very-long-chain 3-ketoacyl-CoA synthase activity