Bhi05G000571 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000571
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	HECT-type E3 ubiquitin transferase
Location	chr5: 23164559 .. 23165080 (+)
RNA-Seq Expression	Bhi05G000571
Synteny	Bhi05G000571

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATAATATTTATGACCTATTTTATTTTATTTTTAATATGCAAGGAGATTTCCATCATTGGGTTGACCTCTTTAATCATTTCAATTCATTTTTTGAGAAGCATATAAAGAGAAGGAAGGATTTGCAGGTTGAGGATAATTTTGTGGGCTCCGATCCTCCATTTCCAAGAGATGTGCCAACTAGTTTGCGATTTTCTCTTCTAACAAGGATGCGATTTGCAAGAGCATTTCATTCTCTATCTGCTCGGCAGCAATATACCTGCATTCGCTTGTATGCCTTCATTGTTCTTGTTCAAGCAAGTGGCGATGCTGATGATCTAGTTAGTTTCTTTAACTCTGAGCCTGAGTTTGTAAATGAGTTAGTTGGCTTATTAAGCTATGAGGATGAGGTTCCTGTAAAAATACGAATCCTTTGTCTATTATCATTGGTTGCACTTTGTTTGGACCGGTTGCGCCAACCAACTGTGTTGTCTGCAGTGACTTCTGGTTGGCACTGTGGCATCTTGTCCAGCCTCATGTAG

mRNA sequence

ATGCATAATATTTATGACCTATTTTATTTTATTTTTAATATGCAAGGAGATTTCCATCATTGGGTTGACCTCTTTAATCATTTCAATTCATTTTTTGAGAAGCATATAAAGAGAAGGAAGGATTTGCAGGTTGAGGATAATTTTGTGGGCTCCGATCCTCCATTTCCAAGAGATGTGCCAACTAGTTTGCGATTTTCTCTTCTAACAAGGATGCGATTTGCAAGAGCATTTCATTCTCTATCTGCTCGGCAGCAATATACCTGCATTCGCTTGTATGCCTTCATTGTTCTTGTTCAAGCAAGTGGCGATGCTGATGATCTAGTTAGTTTCTTTAACTCTGAGCCTGAGTTTGTAAATGAGTTAGTTGGCTTATTAAGCTATGAGGATGAGGTTCCTGTAAAAATACGAATCCTTTGTCTATTATCATTGGTTGCACTTTGTTTGGACCGGTTGCGCCAACCAACTGTGTTGTCTGCAGTGACTTCTGGTTGGCACTGTGGCATCTTGTCCAGCCTCATGTAG

Coding sequence (CDS)

ATGCATAATATTTATGACCTATTTTATTTTATTTTTAATATGCAAGGAGATTTCCATCATTGGGTTGACCTCTTTAATCATTTCAATTCATTTTTTGAGAAGCATATAAAGAGAAGGAAGGATTTGCAGGTTGAGGATAATTTTGTGGGCTCCGATCCTCCATTTCCAAGAGATGTGCCAACTAGTTTGCGATTTTCTCTTCTAACAAGGATGCGATTTGCAAGAGCATTTCATTCTCTATCTGCTCGGCAGCAATATACCTGCATTCGCTTGTATGCCTTCATTGTTCTTGTTCAAGCAAGTGGCGATGCTGATGATCTAGTTAGTTTCTTTAACTCTGAGCCTGAGTTTGTAAATGAGTTAGTTGGCTTATTAAGCTATGAGGATGAGGTTCCTGTAAAAATACGAATCCTTTGTCTATTATCATTGGTTGCACTTTGTTTGGACCGGTTGCGCCAACCAACTGTGTTGTCTGCAGTGACTTCTGGTTGGCACTGTGGCATCTTGTCCAGCCTCATGTAG

Protein sequence

MHNIYDLFYFIFNMQGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRDVPTSLRFSLLTRMRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEFVNELVGLLSYEDEVPVKIRILCLLSLVALCLDRLRQPTVLSAVTSGWHCGILSSLM

Homology

[Close]

BLAST of Bhi05G000571 vs. TAIR 10
Match: AT1G70320.1 (ubiquitin-protein ligase 2 )

HSP 1 Score: 184.1 bits (466), Expect = 9.5e-47
Identity = 122/310 (39.35%), Postives = 139/310 (44.84%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRD---------------- 74
           +GDFHHWVDLF HF++FFEKH+K RKDL++E+ F  SDPPFP+D                
Sbjct: 43  KGDFHHWVDLFYHFDTFFEKHVKVRKDLRIEEEFDESDPPFPKDAVLQVLRVIRLVLENC 102

Query: 75  ------------------------------------------------------------ 134
                                                                       
Sbjct: 103 TNKQFYTSYEQHLSLLLASTDADVVEACLQTLAAFLKRPTGKYSIRDASLNLKLFSLAQG 162

Query: 135 ------------------------------------------------------------ 174
                                                                       
Sbjct: 163 WGGKEEGLGLTSCATEHSCDQLFLQLGCTLLFEFYASDESPSELPGGLQVIHVPDVSMRS 222

[Close]

BLAST of Bhi05G000571 vs. TAIR 10
Match: AT1G55860.1 (ubiquitin-protein ligase 1 )

HSP 1 Score: 176.0 bits (445), Expect = 2.6e-44
Identity = 91/116 (78.45%), Postives = 102/116 (87.93%), Query Frame = 0


Query: 58  DVPTSLRFSLLTRMRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF 117
           +VP SLRF+LLTRMRFARAF SL+ RQQ+TCIRLYAF+VLVQASGD +++VSFFN EPEF
Sbjct: 486 NVPPSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQASGDTENVVSFFNGEPEF 545

Query: 118 VNELVGLLSYEDEVPVKIRILCLLSLVALCLDRLRQPTVLSAVTSGWHCGILSSLM 174
           VNELV L+SYED VP KIRILCLLSLVAL  DR RQPTVL+AVTSG H G+LS LM
Sbjct: 546 VNELVTLVSYEDTVPEKIRILCLLSLVALSQDRTRQPTVLTAVTSGGHRGLLSGLM 601

HSP 2 Score: 84.0 bits (206), Expect = 1.3e-16
Identity = 44/115 (38.26%), Postives = 66/115 (57.39%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRDVPTSLRFSLLTRMRFA 74
           +GDFHHWVDLFN+F++FFEKH++ RKDL +E+NF  SDPPFP+D    +   +   +   
Sbjct: 292 KGDFHHWVDLFNYFDTFFEKHVQVRKDLHIEENFEESDPPFPKDAVLQVLRVIRVVLENC 351

Query: 75  RAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEFVNELVGLLSYED 130
              H  S+ +Q+        + L+ AS DAD + +   +   F+   +G  S  D
Sbjct: 352 TNKHFYSSYEQH--------LSLLLASTDADVVEACLQTLAAFLKRQIGKYSIRD 398

[Close]

BLAST of Bhi05G000571 vs. TAIR 10
Match: AT1G55860.2 (ubiquitin-protein ligase 1 )

HSP 1 Score: 176.0 bits (445), Expect = 2.6e-44
Identity = 91/116 (78.45%), Postives = 102/116 (87.93%), Query Frame = 0


Query: 58  DVPTSLRFSLLTRMRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF 117
           +VP SLRF+LLTRMRFARAF SL+ RQQ+TCIRLYAF+VLVQASGD +++VSFFN EPEF
Sbjct: 237 NVPPSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQASGDTENVVSFFNGEPEF 296

Query: 118 VNELVGLLSYEDEVPVKIRILCLLSLVALCLDRLRQPTVLSAVTSGWHCGILSSLM 174
           VNELV L+SYED VP KIRILCLLSLVAL  DR RQPTVL+AVTSG H G+LS LM
Sbjct: 297 VNELVTLVSYEDTVPEKIRILCLLSLVALSQDRTRQPTVLTAVTSGGHRGLLSGLM 352

HSP 2 Score: 84.0 bits (206), Expect = 1.3e-16
Identity = 44/115 (38.26%), Postives = 66/115 (57.39%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRDVPTSLRFSLLTRMRFA 74
           +GDFHHWVDLFN+F++FFEKH++ RKDL +E+NF  SDPPFP+D    +   +   +   
Sbjct: 43  KGDFHHWVDLFNYFDTFFEKHVQVRKDLHIEENFEESDPPFPKDAVLQVLRVIRVVLENC 102

Query: 75  RAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEFVNELVGLLSYED 130
              H  S+ +Q+        + L+ AS DAD + +   +   F+   +G  S  D
Sbjct: 103 TNKHFYSSYEQH--------LSLLLASTDADVVEACLQTLAAFLKRQIGKYSIRD 149

[Close]

BLAST of Bhi05G000571 vs. ExPASy Swiss-Prot
Match: Q8H0T4 (E3 ubiquitin-protein ligase UPL2 OS=Arabidopsis thaliana OX=3702 GN=UPL2 PE=1 SV=3)

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-45
Identity = 122/310 (39.35%), Postives = 139/310 (44.84%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRD---------------- 74
           +GDFHHWVDLF HF++FFEKH+K RKDL++E+ F  SDPPFP+D                
Sbjct: 43  KGDFHHWVDLFYHFDTFFEKHVKVRKDLRIEEEFDESDPPFPKDAVLQVLRVIRLVLENC 102

Query: 75  ------------------------------------------------------------ 134
                                                                       
Sbjct: 103 TNKQFYTSYEQHLSLLLASTDADVVEACLQTLAAFLKRPTGKYSIRDASLNLKLFSLAQG 162

Query: 135 ------------------------------------------------------------ 174
                                                                       
Sbjct: 163 WGGKEEGLGLTSCATEHSCDQLFLQLGCTLLFEFYASDESPSELPGGLQVIHVPDVSMRS 222

[Close]

BLAST of Bhi05G000571 vs. ExPASy Swiss-Prot
Match: Q8GY23 (E3 ubiquitin-protein ligase UPL1 OS=Arabidopsis thaliana OX=3702 GN=UPL1 PE=1 SV=3)

HSP 1 Score: 176.0 bits (445), Expect = 3.6e-43
Identity = 91/116 (78.45%), Postives = 102/116 (87.93%), Query Frame = 0


Query: 58  DVPTSLRFSLLTRMRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF 117
           +VP SLRF+LLTRMRFARAF SL+ RQQ+TCIRLYAF+VLVQASGD +++VSFFN EPEF
Sbjct: 237 NVPPSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQASGDTENVVSFFNGEPEF 296

Query: 118 VNELVGLLSYEDEVPVKIRILCLLSLVALCLDRLRQPTVLSAVTSGWHCGILSSLM 174
           VNELV L+SYED VP KIRILCLLSLVAL  DR RQPTVL+AVTSG H G+LS LM
Sbjct: 297 VNELVTLVSYEDTVPEKIRILCLLSLVALSQDRTRQPTVLTAVTSGGHRGLLSGLM 352

HSP 2 Score: 84.0 bits (206), Expect = 1.9e-15
Identity = 44/115 (38.26%), Postives = 66/115 (57.39%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRDVPTSLRFSLLTRMRFA 74
           +GDFHHWVDLFN+F++FFEKH++ RKDL +E+NF  SDPPFP+D    +   +   +   
Sbjct: 43  KGDFHHWVDLFNYFDTFFEKHVQVRKDLHIEENFEESDPPFPKDAVLQVLRVIRVVLENC 102

Query: 75  RAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEFVNELVGLLSYED 130
              H  S+ +Q+        + L+ AS DAD + +   +   F+   +G  S  D
Sbjct: 103 TNKHFYSSYEQH--------LSLLLASTDADVVEACLQTLAAFLKRQIGKYSIRD 149

[Close]

BLAST of Bhi05G000571 vs. ExPASy TrEMBL
Match: A0A5D3CU92 (HECT-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00300 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 7.8e-57
Identity = 148/314 (47.13%), Postives = 153/314 (48.73%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRD---------------- 74
           +GDFHHWVDLFNHF+SFFEKHIK RKDLQVEDNF+GSDPPFPR+                
Sbjct: 40  KGDFHHWVDLFNHFDSFFEKHIKTRKDLQVEDNFLGSDPPFPREAVLQILRVIRIILGNC 99

Query: 75  ------------------------------------------------------------ 134
                                                                       
Sbjct: 100 TNKHFYSSYEQHLSCLLASTDADIVEACLQTLSAFLKKSIGKYSIRDASLNSKLFALAQG 159

Query: 135 ------------------------------------------------------------ 174
                                                                       
Sbjct: 160 WGGKEEGLGLIACALQNGCSQVTHELGCTLHFEFYALNESGSDSTEQSAKGLQIIHLPNV 219

[Close]

BLAST of Bhi05G000571 vs. ExPASy TrEMBL
Match: A0A6J1EWH8 (HECT-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111438749 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 4.3e-55
Identity = 145/313 (46.33%), Postives = 150/313 (47.92%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRD---------------- 74
           +GDFHHW DLFNHF+SFFEKHIK RKDLQVEDNF+GSD PFPR+                
Sbjct: 43  KGDFHHWADLFNHFDSFFEKHIKFRKDLQVEDNFLGSDSPFPREAVLQILRVIRIILENC 102

Query: 75  ------------------------------------------------------------ 134
                                                                       
Sbjct: 103 TNKQFYSSYEQHLSCLLASTDADIVEACLQTLAAFLKKSIGKHTIRDASLNSKLFSLAQG 162

Query: 135 ------------------------------------------------------------ 174
                                                                       
Sbjct: 163 WGGKEEGLGLIACALQNGCSQVTHELGCTLHFEFYAINESGSEIEQLAKGLQIIHLSNVS 222

[Close]

BLAST of Bhi05G000571 vs. ExPASy TrEMBL
Match: A0A2N9HIV7 (HECT-type E3 ubiquitin transferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS39513 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 3.1e-53
Identity = 136/291 (46.74%), Postives = 148/291 (50.86%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRD---------------- 74
           +GDFHHWVDLFNHF+SFFEKHIK RKDLQ+ED+F+ SDPPFPR+                
Sbjct: 43  KGDFHHWVDLFNHFDSFFEKHIKLRKDLQIEDDFLDSDPPFPREAQHLSLLLASTDPDVI 102

Query: 75  ------------------------------------------------------------ 134
                                                                       
Sbjct: 103 EACLQTLAAFLKKTIGKYSIRDASLNSKLFALAQGWGGKEEGLGLIATAVQDSCDPIAYD 162

Query: 135 --------------------------------------------------------VPTS 174
                                                                   VPTS
Sbjct: 163 LGCTLHFEFYASSDLSCELPAAEHSTQGLQTIHLPDINARLETDLELLSNLVAEYKVPTS 222

[Close]

BLAST of Bhi05G000571 vs. ExPASy TrEMBL
Match: A0A1S3BL62 (HECT-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491036 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 5.4e-50
Identity = 109/116 (93.97%), Postives = 111/116 (95.69%), Query Frame = 0


Query: 58  DVPTSLRFSLLTRMRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF 117
           +VPTSLRFSLLTR+RFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF
Sbjct: 241 NVPTSLRFSLLTRLRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF 300

Query: 118 VNELVGLLSYEDEVPVKIRILCLLSLVALCLDRLRQPTVLSAVTSGWHCGILSSLM 174
           VNELVGLLSYEDEVPVKIRI CLLSLVALC DR RQPTVLSAVTSG H GILSSLM
Sbjct: 301 VNELVGLLSYEDEVPVKIRIHCLLSLVALCQDRSRQPTVLSAVTSGGHRGILSSLM 356

[Close]

BLAST of Bhi05G000571 vs. ExPASy TrEMBL
Match: A0A1S3BL62 (HECT-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491036 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.8e-16
Identity = 52/115 (45.22%), Postives = 68/115 (59.13%), Query Frame = 0


Query: 15  QGDFHHWVDLFNHFNSFFEKHIKRRKDLQVEDNFVGSDPPFPRDVPTSLRFSLLTRMRFA 74
           +GDFHHWVDLFNHF+SFFEKHIK RKDLQVEDNF+GSDPPFPR+    +   +   +   
Sbjct: 43  KGDFHHWVDLFNHFDSFFEKHIKTRKDLQVEDNFLGSDPPFPREAVLQILRVIRIILGNC 102

Query: 75  RAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEFVNELVGLLSYED 130
              H  S+ +Q+    L        AS DAD + +   +   F+ + +G  S  D
Sbjct: 103 TNKHFYSSYEQHLSCLL--------ASTDADIVEACLQTLSAFLKKSIGKYSIRD 149

HSP 2 Score: 207.2 bits (526), Expect = 5.4e-50
Identity = 109/116 (93.97%), Postives = 111/116 (95.69%), Query Frame = 0


Query: 58  DVPTSLRFSLLTRMRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF 117
           +VPTSLRFSLLTR+RFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF
Sbjct: 237 NVPTSLRFSLLTRLRFARAFHSLSARQQYTCIRLYAFIVLVQASGDADDLVSFFNSEPEF 296

Query: 118 VNELVGLLSYEDEVPVKIRILCLLSLVALCLDRLRQPTVLSAVTSGWHCGILSSLM 174
           VNELVGLLSYEDEVPVKIRI CLLSLVALC DR RQPTVLSAVTSG H GILSSLM
Sbjct: 297 VNELVGLLSYEDEVPVKIRIHCLLSLVALCQDRSRQPTVLSAVTSGGHRGILSSLM 352

The following BLAST results are available for this feature:

BLAST of Bhi05G000571 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70320.1	9.5e-47	39.35	ubiquitin-protein ligase 2	[more]
AT1G55860.1	2.6e-44	78.45	ubiquitin-protein ligase 1	[more]
AT1G55860.2	2.6e-44	78.45	ubiquitin-protein ligase 1	[more]

BLAST of Bhi05G000571 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H0T4	1.3e-45	39.35	E3 ubiquitin-protein ligase UPL2 OS=Arabidopsis thaliana OX=3702 GN=UPL2 PE=1 SV...	[more]
Q8GY23	3.6e-43	78.45	E3 ubiquitin-protein ligase UPL1 OS=Arabidopsis thaliana OX=3702 GN=UPL1 PE=1 SV...	[more]

BLAST of Bhi05G000571 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CU92	7.8e-57	47.13	HECT-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1EWH8	4.3e-55	46.33	HECT-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111438749...	[more]
A0A2N9HIV7	3.1e-53	46.74	HECT-type E3 ubiquitin transferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS39513...	[more]
A0A1S3BL62	5.4e-50	93.97	HECT-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491036 PE=4 ...	[more]
A0A1S3BL62	1.8e-16	45.22	HECT-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491036 PE=4 ...	[more]

Pages

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010309	E3 ubiquitin ligase, domain of unknown function DUF908	PFAM	PF06012	DUF908	coord: 56..172 e-value: 2.1E-21 score: 76.8
None	No IPR available	PANTHER	PTHR11254	HECT DOMAIN UBIQUITIN-PROTEIN LIGASE	coord: 58..147 coord: 15..57
None	No IPR available	PANTHER	PTHR11254:SF398	E3 UBIQUITIN-PROTEIN LIGASE UPL2-LIKE ISOFORM X1	coord: 58..147 coord: 15..57

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000571	Bhi05M000571	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045732	positive regulation of protein catabolic process
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity