Bhi05G000547 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000547
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr5: 22016005 .. 22016232 (-)
RNA-Seq ExpressionBhi05G000547
SyntenyBhi05G000547
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAAGATGGGTTAATTCCTAATGTCTTTACATATAATATACTTTTGAAGGCATTGTGCAAGAATGACCGTGTTGATGCTGCACACAAGCTGTTTGTCGAAATGTCAAGTAACGGCTGCCCACCTTACGTGGTTAACTATACGACTATGGTGTCTTCACTGTGTAAAGCAGGTAAGATTGATGATGCTAGAGAGCTAGCGGGAAGATTTAATGGAAATGTCTAG

mRNA sequence

ATGAAGAAAGATGGGTTAATTCCTAATGTCTTTACATATAATATACTTTTGAAGGCATTGTGCAAGAATGACCGTGTTGATGCTGCACACAAGCTGTTTGTCGAAATGTCAAGTAACGGCTGCCCACCTTACGTGGTTAACTATACGACTATGGTGTCTTCACTGTGTAAAGCAGGTAAGATTGATGATGCTAGAGAGCTAGCGGGAAGATTTAATGGAAATGTCTAG

Coding sequence (CDS)

ATGAAGAAAGATGGGTTAATTCCTAATGTCTTTACATATAATATACTTTTGAAGGCATTGTGCAAGAATGACCGTGTTGATGCTGCACACAAGCTGTTTGTCGAAATGTCAAGTAACGGCTGCCCACCTTACGTGGTTAACTATACGACTATGGTGTCTTCACTGTGTAAAGCAGGTAAGATTGATGATGCTAGAGAGCTAGCGGGAAGATTTAATGGAAATGTCTAG

Protein sequence

MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGKIDDARELAGRFNGNV
Homology
[Close]
BLAST of Bhi05G000547 vs. TAIR 10
Match: AT3G48810.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 2.0e-22
Identity = 45/71 (63.38%), Postives = 57/71 (80.28%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MK+DG  PNVFTYN+LLKALCKN++VD A KL VEMS+ GC P  V+YTT++SS+C+ G 
Sbjct: 172 MKRDGFEPNVFTYNVLLKALCKNNKVDGAKKLLVEMSNKGCCPDAVSYTTVISSMCEVGL 231

Query: 61  IDDARELAGRF 72
           + + RELA RF
Sbjct: 232 VKEGRELAERF 242

[Close]
BLAST of Bhi05G000547 vs. TAIR 10
Match: AT5G46100.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 6.0e-14
Identity = 35/72 (48.61%), Postives = 46/72 (63.89%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MK  G+ PNVFTY+ L+  LCK+ R   A +LF  M + GC P +V YTT+++ LCK  K
Sbjct: 253 MKSKGIEPNVFTYSSLMDGLCKDGRSLQAMELFEMMMARGCRPNMVTYTTLITGLCKEQK 312

Query: 61  IDDARELAGRFN 73
           I +A EL  R N
Sbjct: 313 IQEAVELLDRMN 324

[Close]
BLAST of Bhi05G000547 vs. TAIR 10
Match: AT3G04760.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 6.6e-13
Identity = 30/70 (42.86%), Postives = 47/70 (67.14%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MK+ GL P+ ++Y+ L+ A C+  R+D A +    M S+GC P +VNY T++++LCK GK
Sbjct: 359 MKEKGLTPDAYSYDPLIAAFCREGRLDVAIEFLETMISDGCLPDIVNYNTVLATLCKNGK 418

Query: 61  IDDARELAGR 71
            D A E+ G+
Sbjct: 419 ADQALEIFGK 428

[Close]
BLAST of Bhi05G000547 vs. TAIR 10
Match: AT1G63070.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 66.6 bits (161), Expect = 9.5e-12
Identity = 30/67 (44.78%), Postives = 41/67 (61.19%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           M  DG+ P++ TYNILL  LC N  V+ A  +F  M        +V YTTM+ +LCKAGK
Sbjct: 417 MVSDGVHPDIMTYNILLDGLCNNGNVETALVVFEYMQKRDMKLDIVTYTTMIEALCKAGK 476

Query: 61  IDDAREL 68
           ++D  +L
Sbjct: 477 VEDGWDL 483

[Close]
BLAST of Bhi05G000547 vs. TAIR 10
Match: AT5G65820.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 9.5e-12
Identity = 31/62 (50.00%), Postives = 40/62 (64.52%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           M++ G  PN   Y +L++ALCK DR++ A K+FVEM    C   VV YT +VS  CK GK
Sbjct: 313 MRRRGFEPNANCYTVLIQALCKVDRMEEAMKVFVEMERYECEADVVTYTALVSGFCKWGK 372

Query: 61  ID 63
           ID
Sbjct: 373 ID 374

[Close]
BLAST of Bhi05G000547 vs. ExPASy Swiss-Prot
Match: Q9M302 (Pentatricopeptide repeat-containing protein At3g48810 OS=Arabidopsis thaliana OX=3702 GN=At3g48810 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.9e-21
Identity = 45/71 (63.38%), Postives = 57/71 (80.28%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MK+DG  PNVFTYN+LLKALCKN++VD A KL VEMS+ GC P  V+YTT++SS+C+ G 
Sbjct: 172 MKRDGFEPNVFTYNVLLKALCKNNKVDGAKKLLVEMSNKGCCPDAVSYTTVISSMCEVGL 231

Query: 61  IDDARELAGRF 72
           + + RELA RF
Sbjct: 232 VKEGRELAERF 242

[Close]
BLAST of Bhi05G000547 vs. ExPASy Swiss-Prot
Match: Q9FNL2 (Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX=3702 GN=At5g46100 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 8.4e-13
Identity = 35/72 (48.61%), Postives = 46/72 (63.89%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MK  G+ PNVFTY+ L+  LCK+ R   A +LF  M + GC P +V YTT+++ LCK  K
Sbjct: 253 MKSKGIEPNVFTYSSLMDGLCKDGRSLQAMELFEMMMARGCRPNMVTYTTLITGLCKEQK 312

Query: 61  IDDARELAGRFN 73
           I +A EL  R N
Sbjct: 313 IQEAVELLDRMN 324

[Close]
BLAST of Bhi05G000547 vs. ExPASy Swiss-Prot
Match: Q9SR00 (Pentatricopeptide repeat-containing protein At3g04760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g04760 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 9.3e-12
Identity = 30/70 (42.86%), Postives = 47/70 (67.14%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MK+ GL P+ ++Y+ L+ A C+  R+D A +    M S+GC P +VNY T++++LCK GK
Sbjct: 359 MKEKGLTPDAYSYDPLIAAFCREGRLDVAIEFLETMISDGCLPDIVNYNTVLATLCKNGK 418

Query: 61  IDDARELAGR 71
            D A E+ G+
Sbjct: 419 ADQALEIFGK 428

[Close]
BLAST of Bhi05G000547 vs. ExPASy Swiss-Prot
Match: Q9FIX3 (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX=3702 GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 30/67 (44.78%), Postives = 40/67 (59.70%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           M +  + PNVFTYNIL++  C    +D A  LF +M + GC P VV Y T++   CK  K
Sbjct: 196 MLESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRK 255

Query: 61  IDDAREL 68
           IDD  +L
Sbjct: 256 IDDGFKL 262

[Close]
BLAST of Bhi05G000547 vs. ExPASy Swiss-Prot
Match: Q9FH87 (Putative pentatricopeptide repeat-containing protein At5g65820 OS=Arabidopsis thaliana OX=3702 GN=At5g65820 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 31/62 (50.00%), Postives = 40/62 (64.52%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           M++ G  PN   Y +L++ALCK DR++ A K+FVEM    C   VV YT +VS  CK GK
Sbjct: 313 MRRRGFEPNANCYTVLIQALCKVDRMEEAMKVFVEMERYECEADVVTYTALVSGFCKWGK 372

Query: 61  ID 63
           ID
Sbjct: 373 ID 374

[Close]
BLAST of Bhi05G000547 vs. ExPASy TrEMBL
Match: A0A6J1EVI3 (pentatricopeptide repeat-containing protein At3g48810 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436965 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 5.4e-31
Identity = 68/75 (90.67%), Postives = 70/75 (93.33%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSS GCPP  V+YTTMVSSLCKAGK
Sbjct: 170 MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSKGCPPDAVSYTTMVSSLCKAGK 229

Query: 61  IDDARELAGRFNGNV 76
           IDDARELAGRF  +V
Sbjct: 230 IDDARELAGRFKSSV 244

[Close]
BLAST of Bhi05G000547 vs. ExPASy TrEMBL
Match: A0A6J1EX52 (pentatricopeptide repeat-containing protein At3g48810 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436965 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 5.4e-31
Identity = 68/75 (90.67%), Postives = 70/75 (93.33%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSS GCPP  V+YTTMVSSLCKAGK
Sbjct: 170 MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSKGCPPDAVSYTTMVSSLCKAGK 229

Query: 61  IDDARELAGRFNGNV 76
           IDDARELAGRF  +V
Sbjct: 230 IDDARELAGRFKSSV 244

[Close]
BLAST of Bhi05G000547 vs. ExPASy TrEMBL
Match: A0A6J1KE18 (pentatricopeptide repeat-containing protein At3g48810 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494828 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 9.3e-31
Identity = 68/75 (90.67%), Postives = 70/75 (93.33%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSS GCPP  V+YTTMVSSLCKAGK
Sbjct: 170 MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSKGCPPDAVSYTTMVSSLCKAGK 229

Query: 61  IDDARELAGRFNGNV 76
           IDDARELAGRF  +V
Sbjct: 230 IDDARELAGRFKPSV 244

[Close]
BLAST of Bhi05G000547 vs. ExPASy TrEMBL
Match: A0A6J1KN20 (pentatricopeptide repeat-containing protein At3g48810 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111494828 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 9.3e-31
Identity = 68/75 (90.67%), Postives = 70/75 (93.33%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSS GCPP  V+YTTMVSSLCKAGK
Sbjct: 170 MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSKGCPPDAVSYTTMVSSLCKAGK 229

Query: 61  IDDARELAGRFNGNV 76
           IDDARELAGRF  +V
Sbjct: 230 IDDARELAGRFKPSV 244

[Close]
BLAST of Bhi05G000547 vs. ExPASy TrEMBL
Match: A0A0A0KCK1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G324890 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.7e-30
Identity = 67/75 (89.33%), Postives = 69/75 (92.00%), Query Frame = 0 

Query: 1   MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSSNGCPPYVVNYTTMVSSLCKAGK 60
           MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMS+ GCPP  V YTTMVSSLCKAGK
Sbjct: 170 MKKDGLIPNVFTYNILLKALCKNDRVDAAHKLFVEMSNKGCPPDAVTYTTMVSSLCKAGK 229

Query: 61  IDDARELAGRFNGNV 76
           IDDARELAGRF  +V
Sbjct: 230 IDDARELAGRFKPSV 244

The following BLAST results are available for this feature:
BLAST of Bhi05G000547 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G48810.12.0e-2263.38Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G46100.16.0e-1448.61Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G04760.16.6e-1342.86Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT1G63070.19.5e-1244.78pentatricopeptide (PPR) repeat-containing protein [more]
AT5G65820.19.5e-1250.00Pentatricopeptide repeat (PPR) superfamily protein [more]
BLAST of Bhi05G000547 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M3022.9e-2163.38Pentatricopeptide repeat-containing protein At3g48810 OS=Arabidopsis thaliana OX... [more]
Q9FNL28.4e-1348.61Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX... [more]
Q9SR009.3e-1242.86Pentatricopeptide repeat-containing protein At3g04760, chloroplastic OS=Arabidop... [more]
Q9FIX31.3e-1044.78Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX... [more]
Q9FH871.3e-1050.00Putative pentatricopeptide repeat-containing protein At5g65820 OS=Arabidopsis th... [more]
BLAST of Bhi05G000547 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EVI35.4e-3190.67pentatricopeptide repeat-containing protein At3g48810 isoform X1 OS=Cucurbita mo... [more]
A0A6J1EX525.4e-3190.67pentatricopeptide repeat-containing protein At3g48810 isoform X2 OS=Cucurbita mo... [more]
A0A6J1KE189.3e-3190.67pentatricopeptide repeat-containing protein At3g48810 isoform X1 OS=Cucurbita ma... [more]
A0A6J1KN209.3e-3190.67pentatricopeptide repeat-containing protein At3g48810 isoform X2 OS=Cucurbita ma... [more]
A0A0A0KCK12.7e-3089.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G324890 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..72
e-value: 2.3E-23
score: 84.7
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 11..43
e-value: 8.0E-12
score: 42.6
coord: 48..67
e-value: 1.9E-5
score: 22.5
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 8..57
e-value: 1.4E-16
score: 60.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 9..43
score: 13.822257
NoneNo IPR availablePANTHERPTHR46128MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1coord: 1..72
NoneNo IPR availablePANTHERPTHR46128:SF195PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 1..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000547Bhi05M000547mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity