Bhi05G000543 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000543
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr5: 21859678 .. 21860088 (+)
RNA-Seq ExpressionBhi05G000543
SyntenyBhi05G000543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTACAATTATGCCCTTACTCAATGCATTGCACAGCTGTGGCATGGGCTGTCGAAGACGCTAGCAGAGAAGACCGCGTGGGCATTAGCTATGGACAGAGGAGTCAATATGGTATCGGTCAACGCAGGGCTATTGGTGGGCCCTGGTTTGACCATTGATCATCCGTATTTGAATGGAGCGGCTGAGATGTACGAGGGTGGATTATTTGTGACCGTTGATTTGAATTACTTGGTGGACGCTCACATTCGCGTTTTCAAAGATCTCTCATCCTACGGTCGTTATATATGCTTCAATCACATCATTAATACGCATGATGATGCAGTTGATCTCGCCCGCAAATTGACGCCTTTGCCCCTAAGGTTATTGTCCTCGTCATGGATTTTTTTTTCTCACTTTTACTTCTGTTAG

mRNA sequence

ATGACTTACAATTATGCCCTTACTCAATGCATTGCACAGCTGTGGCATGGGCTGTCGAAGACGCTAGCAGAGAAGACCGCGTGGGCATTAGCTATGGACAGAGGAGTCAATATGGTATCGGTCAACGCAGGGCTATTGGTGGGCCCTGGTTTGACCATTGATCATCCGTATTTGAATGGAGCGGCTGAGATGTACGAGGGTGGATTATTTGTGACCGTTGATTTGAATTACTTGGTGGACGCTCACATTCGCGTTTTCAAAGATCTCTCATCCTACGGTCGTTATATATGCTTCAATCACATCATTAATACGCATGATGATGCAGTTGATCTCGCCCGCAAATTGACGCCTTTGCCCCTAAGGTTATTGTCCTCGTCATGGATTTTTTTTTCTCACTTTTACTTCTGTTAG

Coding sequence (CDS)

ATGACTTACAATTATGCCCTTACTCAATGCATTGCACAGCTGTGGCATGGGCTGTCGAAGACGCTAGCAGAGAAGACCGCGTGGGCATTAGCTATGGACAGAGGAGTCAATATGGTATCGGTCAACGCAGGGCTATTGGTGGGCCCTGGTTTGACCATTGATCATCCGTATTTGAATGGAGCGGCTGAGATGTACGAGGGTGGATTATTTGTGACCGTTGATTTGAATTACTTGGTGGACGCTCACATTCGCGTTTTCAAAGATCTCTCATCCTACGGTCGTTATATATGCTTCAATCACATCATTAATACGCATGATGATGCAGTTGATCTCGCCCGCAAATTGACGCCTTTGCCCCTAAGGTTATTGTCCTCGTCATGGATTTTTTTTTCTCACTTTTACTTCTGTTAG

Protein sequence

MTYNYALTQCIAQLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLNGAAEMYEGGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPLPLRLLSSSWIFFSHFYFC
Homology
BLAST of Bhi05G000543 vs. TAIR 10
Match: AT2G23910.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 4.4e-31
Identity = 61/111 (54.95%), Postives = 80/111 (72.07%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHP-----YLNGAAEMYEG 72
           +LWH L+KT +EK AWALAMDR VNMVSVN GL+VGP +   +P     YL GAA+MYE 
Sbjct: 158 KLWHALAKTQSEKAAWALAMDRMVNMVSVNPGLIVGPSVAQHNPRPTMSYLKGAAQMYEN 217

Query: 73  GLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPL 119
           G+   VD+ ++ D HIR F+D S+ GRY CFN I+NT ++A+ L + L+PL
Sbjct: 218 GVLAYVDVEFVADVHIRAFEDTSACGRYFCFNQIVNTEEEALKLVQTLSPL 268

BLAST of Bhi05G000543 vs. TAIR 10
Match: AT4G30470.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 2.8e-30
Identity = 59/111 (53.15%), Postives = 79/111 (71.17%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDH-----PYLNGAAEMYEG 72
           +LWH L+K L+EK AWALAMDR +NMVS+N GL+VGP +   +      YL GAA+MYE 
Sbjct: 157 KLWHALAKMLSEKAAWALAMDRRLNMVSINPGLVVGPSVAQHNARPTMSYLKGAAQMYEN 216

Query: 73  GLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPL 119
           G+   VD+ +L D HIR F+D+S+ GRY CFN I+NT ++A+ L   L+PL
Sbjct: 217 GVLAYVDVKFLADVHIRAFEDVSACGRYFCFNQIVNTEEEALKLVESLSPL 267

BLAST of Bhi05G000543 vs. TAIR 10
Match: AT5G14700.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 2.6e-15
Identity = 42/111 (37.84%), Postives = 65/111 (58.56%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGL-----TIDHPYLNGAAEMYEG 72
           +LW+ L K  AEK AW +A  +G+ + ++   L+ GP       T    YL GA EMY  
Sbjct: 213 KLWYALGKLKAEKAAWRIADSKGLKLATICPALITGPDFFNRNSTSTLAYLKGAKEMYSN 272

Query: 73  GLFVTVDLNYLVDAHIRVFKDL---SSYGRYICFNHIINTHDDAVDLARKL 116
           GL  T+D+N L  AH+ +++ L   +++GRYICF+ I+ + D A  LA+ +
Sbjct: 273 GLLATMDVNRLAKAHVCLWEGLGNKTAFGRYICFDTIL-SRDGAEKLAKDI 322

BLAST of Bhi05G000543 vs. TAIR 10
Match: AT5G19440.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 7.8e-12
Identity = 40/120 (33.33%), Postives = 70/120 (58.33%), Query Frame = 0

Query: 12  AQLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLN----------GA 71
           +++W+ LSKTLAE  AW LA ++G+++V++N  +++GP L    P LN            
Sbjct: 161 SKMWYVLSKTLAEDAAWKLAKEKGLDIVTINPAMVIGPLL---QPTLNTSAAAILNLING 220

Query: 72  AEMYEGGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTP-LPL 121
           A+ +    F  V++  + +AHI+ F+  S+ GRY     +++ H + V++ R+L P LPL
Sbjct: 221 AKTFPNLSFGWVNVKDVANAHIQAFEVPSANGRYCLVERVVH-HSEIVNILRELYPNLPL 276

BLAST of Bhi05G000543 vs. TAIR 10
Match: AT5G58490.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 5.0e-11
Identity = 40/110 (36.36%), Postives = 57/110 (51.82%), Query Frame = 0

Query: 14  LWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLN-----------GAA 73
           LW+ LSKTLAEK AW  A ++G+++V VN G ++GP   +  P LN           G  
Sbjct: 161 LWYPLSKTLAEKAAWEFAEEKGLDVVVVNPGTVMGP---VIPPSLNASMHMLLRLLQGCT 220

Query: 74  EMYEGGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLA 113
           E YE     +V    +  AHI V++D  S GR++C   I +  D    +A
Sbjct: 221 ETYENFFMGSVHFKDVALAHILVYEDPYSKGRHLCVEAISHYGDFVAKVA 267

BLAST of Bhi05G000543 vs. ExPASy Swiss-Prot
Match: Q500U8 (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.2e-10
Identity = 38/117 (32.48%), Postives = 63/117 (53.85%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTID--------HPYLNGAAEM 72
           Q+W+ LSKTLAE+ AW  + + G+++V+V    LVGP L  D           L G  E 
Sbjct: 159 QVWYALSKTLAEQAAWKFSEENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEK 218

Query: 73  YE-GGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPLPL 121
           ++  G    V ++ +   HI VF+  ++ GRYIC +++I+  +    L+ +   LP+
Sbjct: 219 FQWHGQMGYVHIDDVARTHIVVFEHEAAQGRYICSSNVISLEELVSFLSARYPSLPI 275

BLAST of Bhi05G000543 vs. ExPASy Swiss-Prot
Match: A0A0B6VQ48 (Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.7e-08
Identity = 34/116 (29.31%), Postives = 61/116 (52.59%), Query Frame = 0

Query: 12  AQLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLNGA---------- 71
           ++LW+ LSKTLAE  AW    ++G++MV++N  +++GP L    P LN +          
Sbjct: 157 SKLWYVLSKTLAEDAAWKFVKEKGIDMVTINPAMVIGPLL---QPTLNTSAAAILNIIKG 216

Query: 72  AEMYEGGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTP 118
           A  Y    F  +++  + +AH++ F+  S+ GRY C    +    + + +  +L P
Sbjct: 217 ARTYPNASFGWINVKDVANAHVQAFEIPSASGRY-CLVERVAHFTEVLQIIHELYP 268

BLAST of Bhi05G000543 vs. ExPASy Swiss-Prot
Match: A0A059TC02 (Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.3e-08
Identity = 34/111 (30.63%), Postives = 60/111 (54.05%), Query Frame = 0

Query: 15  WHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGL------TIDH--PYLNGAAEMYE 74
           W+   K +AE+ AW  A ++GV++V +N  L+ GP L      ++ H   YL G+A+ Y 
Sbjct: 156 WYCYGKMVAEQAAWEEAKEKGVDLVVINPVLVQGPLLQTTVNASVLHILKYLTGSAKTYA 215

Query: 75  GGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTP 118
             +   VD+  +  AHI +++   + GRY+C   +++   D V++  K  P
Sbjct: 216 NSVQAYVDVKDVALAHILLYETPEASGRYLCAESVLH-RGDVVEILSKFFP 265

BLAST of Bhi05G000543 vs. ExPASy Swiss-Prot
Match: Q9SAH9 (Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 5.6e-07
Identity = 36/114 (31.58%), Postives = 59/114 (51.75%), Query Frame = 0

Query: 15  WHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGL------TIDH--PYLNGAAEMYE 74
           W+   K LAE++AW  A  +GV++V +N  L++GP L      ++ H   YL G+A+ Y 
Sbjct: 155 WYCYGKMLAEQSAWETAKAKGVDLVVLNPVLVLGPPLQSAINASLVHILKYLTGSAKTYA 214

Query: 75  GGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPLPL 121
               V VD+  +   H+ V++  S+ GRYI     ++  +    LA+     PL
Sbjct: 215 NLTQVYVDVRDVALGHVLVYEAPSASGRYILAETALHRGEVVEILAKFFPEYPL 268

BLAST of Bhi05G000543 vs. ExPASy Swiss-Prot
Match: Q9S9N9 (Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.3e-06
Identity = 34/111 (30.63%), Postives = 58/111 (52.25%), Query Frame = 0

Query: 15  WHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGL------TIDH--PYLNGAAEMYE 74
           W+   K +AE+ AW  A ++GV++V +N  L++GP L      ++ H   YL G+A+ Y 
Sbjct: 160 WYCYGKMVAEQAAWETAKEKGVDLVVLNPVLVLGPPLQPTINASLYHVLKYLTGSAKTYA 219

Query: 75  GGLFVTVDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTP 118
                 VD+  +  AH+ V++  S+ GRY+          + V++  KL P
Sbjct: 220 NLTQAYVDVRDVALAHVLVYEAPSASGRYL-LAESARHRGEVVEILAKLFP 269

BLAST of Bhi05G000543 vs. ExPASy TrEMBL
Match: A0A6J1EJE4 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111434692 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-47
Identity = 94/107 (87.85%), Postives = 101/107 (94.39%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLNGAAEMYEGGLFVT 72
           +LWHGL+KTLAEKTAWALA DRGVNMVSVNAGLLVGP LTID+PYL GAAEMYE GLFVT
Sbjct: 161 KLWHGLAKTLAEKTAWALARDRGVNMVSVNAGLLVGPDLTIDNPYLKGAAEMYESGLFVT 220

Query: 73  VDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPLP 120
           VDLNYLV+AHIRVF+DLSSYGRYICFNH+INT+DDA DLARKLT LP
Sbjct: 221 VDLNYLVEAHIRVFQDLSSYGRYICFNHVINTNDDAADLARKLTSLP 267

BLAST of Bhi05G000543 vs. ExPASy TrEMBL
Match: A0A6J1JN40 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111486702 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.5e-47
Identity = 95/107 (88.79%), Postives = 100/107 (93.46%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLNGAAEMYEGGLFVT 72
           +LWHGLSKTLAEKTAWALA DRGVNMVSVNAGLLVGP LTID+PYL GAAEMYE GLFVT
Sbjct: 161 KLWHGLSKTLAEKTAWALARDRGVNMVSVNAGLLVGPDLTIDNPYLKGAAEMYESGLFVT 220

Query: 73  VDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPLP 120
           VDLNYLV+AHIRVF+DLSSYGRYICFNH INT+DDA DLARKLT LP
Sbjct: 221 VDLNYLVEAHIRVFQDLSSYGRYICFNHAINTNDDAADLARKLTSLP 267

BLAST of Bhi05G000543 vs. ExPASy TrEMBL
Match: A0A6J1DUU8 (cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111023700 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 8.3e-46
Identity = 93/107 (86.92%), Postives = 100/107 (93.46%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLNGAAEMYEGGLFVT 72
           +LWHGLSKTLAEKTAWALAMDRGVNMVSV AGLLVGP LTID+PYL GAAEMYE GLFVT
Sbjct: 109 KLWHGLSKTLAEKTAWALAMDRGVNMVSVKAGLLVGPDLTIDNPYLKGAAEMYERGLFVT 168

Query: 73  VDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTPLP 120
           VDL+YLV+AHI VF+D SSYGRYICFN+IINTH+DAVDLARKLT LP
Sbjct: 169 VDLSYLVEAHICVFQDASSYGRYICFNNIINTHEDAVDLARKLTSLP 215

BLAST of Bhi05G000543 vs. ExPASy TrEMBL
Match: A0A0B2P6M0 (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Glycine soja OX=3848 GN=D0Y65_000168 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 3.3e-42
Identity = 82/105 (78.10%), Postives = 96/105 (91.43%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLNGAAEMYEGGLFVT 72
           +LWHG+SKT+AEK+AWALAMDRGVNMVS+NAGLL+   L++ HPYL GAAEMYE G+FVT
Sbjct: 160 KLWHGVSKTMAEKSAWALAMDRGVNMVSINAGLLMAHDLSVKHPYLRGAAEMYEDGVFVT 219

Query: 73  VDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTP 118
           VDL +LVDAHI V++D+SSYGRY+CFNHIINTHDDAV LARKLTP
Sbjct: 220 VDLGFLVDAHICVYEDVSSYGRYLCFNHIINTHDDAVQLARKLTP 264

BLAST of Bhi05G000543 vs. ExPASy TrEMBL
Match: I1J4U4 (3Beta_HSD domain-containing protein OS=Glycine max OX=3847 GN=100790333 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 3.3e-42
Identity = 82/105 (78.10%), Postives = 96/105 (91.43%), Query Frame = 0

Query: 13  QLWHGLSKTLAEKTAWALAMDRGVNMVSVNAGLLVGPGLTIDHPYLNGAAEMYEGGLFVT 72
           +LWHG+SKT+AEK+AWALAMDRGVNMVS+NAGLL+   L++ HPYL GAAEMYE G+FVT
Sbjct: 160 KLWHGVSKTMAEKSAWALAMDRGVNMVSINAGLLMAHDLSVKHPYLRGAAEMYEDGVFVT 219

Query: 73  VDLNYLVDAHIRVFKDLSSYGRYICFNHIINTHDDAVDLARKLTP 118
           VDL +LVDAHI V++D+SSYGRY+CFNHIINTHDDAV LARKLTP
Sbjct: 220 VDLGFLVDAHICVYEDVSSYGRYLCFNHIINTHDDAVQLARKLTP 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G23910.14.4e-3154.95NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G30470.12.8e-3053.15NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G14700.12.6e-1537.84NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G19440.17.8e-1233.33NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G58490.15.0e-1136.36NAD(P)-binding Rossmann-fold superfamily protein [more]
Match NameE-valueIdentityDescription
Q500U84.2e-1032.48Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE... [more]
A0A0B6VQ481.7e-0829.31Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1[more]
A0A059TC022.3e-0830.63Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1[more]
Q9SAH95.6e-0731.58Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1[more]
Q9S9N91.3e-0630.63Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EJE41.2e-4787.85cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111434692 ... [more]
A0A6J1JN401.5e-4788.79cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111486702 PE... [more]
A0A6J1DUU88.3e-4686.92cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111023700... [more]
A0A0B2P6M03.3e-4278.10Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Glycine soja O... [more]
I1J4U43.3e-4278.103Beta_HSD domain-containing protein OS=Glycine max OX=3847 GN=100790333 PE=4 SV=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 4..125
e-value: 6.1E-19
score: 70.3
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 12..119
NoneNo IPR availablePANTHERPTHR10366:SF740SUBFAMILY NOT NAMEDcoord: 12..119
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 12..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000543Bhi05M000543mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity