Bhi05G000538 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000538
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDUF4283 domain-containing protein
Locationchr5: 21653258 .. 21654502 (-)
RNA-Seq ExpressionBhi05G000538
SyntenyBhi05G000538
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCTCCCATCGGCCCTCCCTTCCAAGTCTAATTCAGCCGTCGAAGTTATAAGTTGTAGGATTAATGACAGCCATTTCTGTGCCCTCCTCCAAGCTGGTTCTGTTATCATAGAAGACATGAAAGCCAAGAAGCAAATCTCCTTGGCAAAATCGCAGCTACTTTGGTTTGCGGAAAACATTTCAGCCCTTTCGAAGCTTCCAACCTCCAGCTTCTTTGTCAAAAGCGGTCAAGGTGCATTCGGGGTAACCAAGATATCAAAGATCAGAGTCTACTCAAAATGGTTTTTAAGATGTGTCGTGTGGCCTGCAGTTGGAGGATGTTTCTTCATCCATGTTCCATCAGGAACCCTTAATTTAGGTTGGCATTCCTTCTTTAACATGTTAAGTGATGCATTGTCTTTATTTGAAGCTGGTCGGGAGACAAAAATCCCCCTCATCCCGAACTCCTATGCAGACATAGCAAGGGGTAGAAAATCCTTGGATAAGTCAGCCTTTTCAGACAAACAGACTCCCCTGCCCCCTCAGATCACTAAAAAACAGAGCAAAACTGATTTTTGGGTTAAAAAAAACCAAGATGTCCTCAAGGAAGATTTCAGCCATTTGTGGGTAGTCAGTCGGTTTTGTGAATTCAATGAATGGTCAGAAGTCTCAAAGGAAGTGAAACTTTGGTCAAATTCTAAAACTATCATCAATCCTCTTTCAGCGGAATCAACCTTAATAAAGTTGCAAAAGGGGGAATTGGAAGATTTGATTGAACATCCTGGAGTGTGGCAAAAGATTGGACAGCTTCATCTAAAATTTGAAAGGTGGAACACCACCTTACACAGCCGCCCAAGGTTAATTAAAGGTTATGGAGGCTGGATTTCAATTAAAAACCTGCCACTAGAGTTTTGGTGCAAGCAAATTTTCGAAGCAATAGGTTCATATTTTGGAGGATTAGAAGCCATTGCTATAGAAACTTTAAACCTCCTAAATGTGACAGAAGCTAGAATTCAAGTCAAGAAGAATTTATGTGGGTTTTTACCAACAACTTTAGAAGTTTCAGATGAAAACAGGGGAAGGATTTTTCTACATTTTGGTGATATTGAGTTCCTAGACCCTCTAAAGAAGGCCAAGAATTTTTTTAATGAAAAGAACCTCTCAAATCCGGTAGACAAGGCTAGATTTAATCAAGTTTTGATAGATGAAGGATTAATAAATTCAAATGAGTTTGAAGAATGGTCCTTTCAAGTCAACAGTTAA

mRNA sequence

ATGGCCCTCCCATCGGCCCTCCCTTCCAAGTCTAATTCAGCCGTCGAAGTTATAAGTTGTAGGATTAATGACAGCCATTTCTGTGCCCTCCTCCAAGCTGGTTCTGTTATCATAGAAGACATGAAAGCCAAGAAGCAAATCTCCTTGGCAAAATCGCAGCTACTTTGGTTTGCGGAAAACATTTCAGCCCTTTCGAAGCTTCCAACCTCCAGCTTCTTTGTCAAAAGCGGTCAAGGTGCATTCGGGGTAACCAAGATATCAAAGATCAGAGTCTACTCAAAATGGTTTTTAAGATGTGTCGTGTGGCCTGCAGTTGGAGGATGTTTCTTCATCCATGTTCCATCAGGAACCCTTAATTTAGGTTGGCATTCCTTCTTTAACATGTTAAGTGATGCATTGTCTTTATTTGAAGCTGGTCGGGAGACAAAAATCCCCCTCATCCCGAACTCCTATGCAGACATAGCAAGGGGTAGAAAATCCTTGGATAAGTCAGCCTTTTCAGACAAACAGACTCCCCTGCCCCCTCAGATCACTAAAAAACAGAGCAAAACTGATTTTTGGGTTAAAAAAAACCAAGATGTCCTCAAGGAAGATTTCAGCCATTTGTGGGTAGTCAGTCGGTTTTGTGAATTCAATGAATGGTCAGAAGTCTCAAAGGAAGTGAAACTTTGGTCAAATTCTAAAACTATCATCAATCCTCTTTCAGCGGAATCAACCTTAATAAAGTTGCAAAAGGGGGAATTGGAAGATTTGATTGAACATCCTGGAGTGTGGCAAAAGATTGGACAGCTTCATCTAAAATTTGAAAGGTGGAACACCACCTTACACAGCCGCCCAAGGTTAATTAAAGGTTATGGAGGCTGGATTTCAATTAAAAACCTGCCACTAGAGTTTTGGTGCAAGCAAATTTTCGAAGCAATAGGTTCATATTTTGGAGGATTAGAAGCCATTGCTATAGAAACTTTAAACCTCCTAAATGTGACAGAAGCTAGAATTCAAGTCAAGAAGAATTTATGTGGGTTTTTACCAACAACTTTAGAAGTTTCAGATGAAAACAGGGGAAGGATTTTTCTACATTTTGGTGATATTGAGTTCCTAGACCCTCTAAAGAAGGCCAAGAATTTTTTTAATGAAAAGAACCTCTCAAATCCGGTAGACAAGGCTAGATTTAATCAAGTTTTGATAGATGAAGGATTAATAAATTCAAATGAGTTTGAAGAATGGTCCTTTCAAGTCAACAGTTAA

Coding sequence (CDS)

ATGGCCCTCCCATCGGCCCTCCCTTCCAAGTCTAATTCAGCCGTCGAAGTTATAAGTTGTAGGATTAATGACAGCCATTTCTGTGCCCTCCTCCAAGCTGGTTCTGTTATCATAGAAGACATGAAAGCCAAGAAGCAAATCTCCTTGGCAAAATCGCAGCTACTTTGGTTTGCGGAAAACATTTCAGCCCTTTCGAAGCTTCCAACCTCCAGCTTCTTTGTCAAAAGCGGTCAAGGTGCATTCGGGGTAACCAAGATATCAAAGATCAGAGTCTACTCAAAATGGTTTTTAAGATGTGTCGTGTGGCCTGCAGTTGGAGGATGTTTCTTCATCCATGTTCCATCAGGAACCCTTAATTTAGGTTGGCATTCCTTCTTTAACATGTTAAGTGATGCATTGTCTTTATTTGAAGCTGGTCGGGAGACAAAAATCCCCCTCATCCCGAACTCCTATGCAGACATAGCAAGGGGTAGAAAATCCTTGGATAAGTCAGCCTTTTCAGACAAACAGACTCCCCTGCCCCCTCAGATCACTAAAAAACAGAGCAAAACTGATTTTTGGGTTAAAAAAAACCAAGATGTCCTCAAGGAAGATTTCAGCCATTTGTGGGTAGTCAGTCGGTTTTGTGAATTCAATGAATGGTCAGAAGTCTCAAAGGAAGTGAAACTTTGGTCAAATTCTAAAACTATCATCAATCCTCTTTCAGCGGAATCAACCTTAATAAAGTTGCAAAAGGGGGAATTGGAAGATTTGATTGAACATCCTGGAGTGTGGCAAAAGATTGGACAGCTTCATCTAAAATTTGAAAGGTGGAACACCACCTTACACAGCCGCCCAAGGTTAATTAAAGGTTATGGAGGCTGGATTTCAATTAAAAACCTGCCACTAGAGTTTTGGTGCAAGCAAATTTTCGAAGCAATAGGTTCATATTTTGGAGGATTAGAAGCCATTGCTATAGAAACTTTAAACCTCCTAAATGTGACAGAAGCTAGAATTCAAGTCAAGAAGAATTTATGTGGGTTTTTACCAACAACTTTAGAAGTTTCAGATGAAAACAGGGGAAGGATTTTTCTACATTTTGGTGATATTGAGTTCCTAGACCCTCTAAAGAAGGCCAAGAATTTTTTTAATGAAAAGAACCTCTCAAATCCGGTAGACAAGGCTAGATTTAATCAAGTTTTGATAGATGAAGGATTAATAAATTCAAATGAGTTTGAAGAATGGTCCTTTCAAGTCAACAGTTAA

Protein sequence

MALPSALPSKSNSAVEVISCRINDSHFCALLQAGSVIIEDMKAKKQISLAKSQLLWFAENISALSKLPTSSFFVKSGQGAFGVTKISKIRVYSKWFLRCVVWPAVGGCFFIHVPSGTLNLGWHSFFNMLSDALSLFEAGRETKIPLIPNSYADIARGRKSLDKSAFSDKQTPLPPQITKKQSKTDFWVKKNQDVLKEDFSHLWVVSRFCEFNEWSEVSKEVKLWSNSKTIINPLSAESTLIKLQKGELEDLIEHPGVWQKIGQLHLKFERWNTTLHSRPRLIKGYGGWISIKNLPLEFWCKQIFEAIGSYFGGLEAIAIETLNLLNVTEARIQVKKNLCGFLPTTLEVSDENRGRIFLHFGDIEFLDPLKKAKNFFNEKNLSNPVDKARFNQVLIDEGLINSNEFEEWSFQVNS
Homology
BLAST of Bhi05G000538 vs. ExPASy TrEMBL
Match: A0A5A7V878 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold828G00460 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 4.6e-39
Identity = 106/330 (32.12%), Postives = 159/330 (48.18%), Query Frame = 0

Query: 82  GVTKISKIRVYSKWFLRCVVWPAVGGCFFIHVPSGTLNLGWHSFFNMLSDALSLFEAGRE 141
           G++K   +  Y  W LRC VWP  GG F++H+P G    GW SF NM+ D LS+  +  +
Sbjct: 3   GLSKSPNLLFYG-WVLRCTVWPESGGRFYLHLPVGPNQQGWISFANMIKDFLSVSTSKED 62

Query: 142 TKIPLIPNSYADIARGRKSLDKSAFSDKQTPLPPQITKKQSKTDFWVKKNQDVLKEDFSH 201
            ++           +G   L+  AFS      P      + K+  WV KN +VL+EDF  
Sbjct: 63  IRM-----------KGVDMLELPAFSQSMLEFP----AFKGKSSSWVHKNSEVLREDF-- 122

Query: 202 LWVVSRFCEFNEWSEVSKEVKLWSNSKTIINPLSAESTLIKLQKGELEDLIEHPGVWQKI 261
                                     K+I++                             
Sbjct: 123 --------------------------KSILD----------------------------- 182

Query: 262 GQLHLKFERWNTTLHSRPRLIKGYGGWISIKNLPLEFWCKQIFEAIGSYFGGLEAIAIET 321
                              ++KGYGGWISIKNLPL++W   +++AIG +FGG E+I+++T
Sbjct: 183 -------------------VVKGYGGWISIKNLPLDYWSIDVYKAIGGFFGGFESISLKT 240

Query: 322 LNLLNVTEARIQVKKNLCGFLPTTLEVSDENRGRIFLHFGDIEFLDPLKKAKNFFNEKNL 381
           +NL+N +EA+I+V +NLCGFLP  +E+ D  R  IFL+FGDI+ L+  K  +       +
Sbjct: 243 MNLINCSEAKIKVSQNLCGFLPAMVELRDAFRDNIFLNFGDIKVLEAPKVIEEALFISYV 240

Query: 382 SNPVDKARFNQVLIDEGLINSNEFE-EWSF 411
           +N +D  R NQVL+DEG  + +  + EW+F
Sbjct: 303 NNLIDLLRINQVLLDEGGESPDMMKLEWAF 240

BLAST of Bhi05G000538 vs. ExPASy TrEMBL
Match: A0A5D3BI91 (Putative 3,4-dihydroxy-2-butanone kinase isoform X5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00280 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 6.3e-36
Identity = 70/121 (57.85%), Postives = 87/121 (71.90%), Query Frame = 0

Query: 253 EHPGVWQKIGQLHLKFERWNTTLHSRPRLIKGYGGWISIKNLPLEFWCKQIFEAIGSYFG 312
           +  G WQ  G  HLK ERW+   HS P + KGYGGW+ IKNLPL++WC+   E IG +F 
Sbjct: 184 DQQGKWQTWGNFHLKIERWDNLKHSWPLVQKGYGGWLKIKNLPLDYWCRSTLEVIGDHFR 243

Query: 313 GLEAIAIETLNLLNVTEARIQVKKNLCGFLPTTLEVSDENRGRIFLHFGDIEFLDPLKKA 372
           GL  IA ETLNL NV+EARIQVKKNLCGF+P+T+E++D     I+LHFGD EFL+P   +
Sbjct: 244 GLTDIAFETLNLTNVSEARIQVKKNLCGFVPSTIEITDLKCRNIYLHFGDFEFLNPTGPS 303

Query: 373 K 374
           K
Sbjct: 304 K 304

BLAST of Bhi05G000538 vs. ExPASy TrEMBL
Match: A0A5A7U128 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1280G00090 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.8e-35
Identity = 82/224 (36.61%), Postives = 123/224 (54.91%), Query Frame = 0

Query: 175 PQITKKQSKTDFWVKKNQDVLKEDFSHLWVVSRFCEFNEWSEVSKEVKLWSNSKTIINPL 234
           P       K   WV +N +V+  +F +LW++++   F++  ++ K ++ +  +K +INPL
Sbjct: 39  PSSNLNTDKQKQWVIRNSEVISTNFENLWIITKLFAFDDRRKIRKMLENYFQAKIVINPL 98

Query: 235 SAESTLIKLQKGELEDLIEHPGVWQKIGQLHLKFERWNTTLHSRPRLIKGYGGWISIKNL 294
             E+ LI L +G ++DLI + G WQ +G  +LKFE+W+   +SRP  +KGYGGW+ IKNL
Sbjct: 99  FDENALINLDEGSIKDLICNEGKWQVLGSFYLKFEKWDKHKYSRPLTMKGYGGWLKIKNL 158

Query: 295 PLEFWCKQIFEAIGSYFGGLEAIAIETLNLLNVTEARIQVKKNLCGFLPTTLEVSDENRG 354
               W     E                      +EARIQVK NLCGF+P+T+E+ D  RG
Sbjct: 159 ---LWITGAGEP--------------------SSEARIQVKHNLCGFVPSTIEIIDPKRG 218

Query: 355 RIFLHFGDIEFLDPLKKAKNFFNEKNLSNPVDKARFNQVLIDEG 399
            IFL+FGD EFL+P     +     +    +   R  +VL DEG
Sbjct: 219 NIFLNFGDFEFLNPPFPTSDTIMVSDFKKSIHLLRIMEVLQDEG 239

BLAST of Bhi05G000538 vs. ExPASy TrEMBL
Match: A0A5D3DVS9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold325G00720 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 2.7e-31
Identity = 66/128 (51.56%), Postives = 88/128 (68.75%), Query Frame = 0

Query: 272 NTTLHSRPRLIKGYGGWISIKNLPLEFWCKQIFEAIGSYFGGLEAIAIETLNLLNVTEAR 331
           N   HSR  ++KGY GW+ IKNLPL+ WC+  FE IG++  GL  IAIETLNL N ++AR
Sbjct: 35  NNIKHSRSLVLKGYDGWLKIKNLPLDDWCRSTFEVIGNHLRGLIDIAIETLNLTNCSDAR 94

Query: 332 IQVKKNLCGFLPTTLEVSDENRGRIFLHFGDIEFLDPLKKAKNFFNEKNLSNPVDKARFN 391
           IQV KNLCGF+ +T E++D  RG I+LHFGD EFL+P + +K      +  N +D+ +  
Sbjct: 95  IQVDKNLCGFVLSTFEITDLKRGNIYLHFGDFEFLNPPRPSKVLILLDDFKNSIDRLKIR 154

Query: 392 QVLIDEGL 400
            VL+DE L
Sbjct: 155 DVLLDEDL 162

BLAST of Bhi05G000538 vs. ExPASy TrEMBL
Match: A0A5D3E3A5 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00110 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.3e-30
Identity = 90/269 (33.46%), Postives = 132/269 (49.07%), Query Frame = 0

Query: 73  FVKSGQGAFGVTKISKIRVYSKWFLRCVVWPAVGGCFFIHVPSGTLNLGWHSFFNMLSDA 132
           F ++G+ A G T+++K +  S W LRCV     G   F+HV SGT   GW S        
Sbjct: 67  FSENGKDASGATRLTKFKSPSGWILRCVTRDFSGHLSFVHVFSGTAKQGWIS-------- 126

Query: 133 LSLFEAGRETKIPLIPNSYADIARGR---KSLDKSAFSDKQTPLPPQITKKQSKTDFWVK 192
                              A   R R   +S++ S    K      ++ K  +K +    
Sbjct: 127 -------------------AQATRTRWSHESINSSVLHFKNRASRHRLLKSSAKIN---- 186

Query: 193 KNQDVLKEDFSHLWVVSRFCEFNEWSEVSKEVKLWSNSKTIINPLSAESTLIKLQKGELE 252
               +L    + + ++  F E  +  EV          K  INP        K+  G++E
Sbjct: 187 ---TLLLTSKTSIRIIIGFLE--DLFEV----------KITINPFFDNRATNKINHGKIE 246

Query: 253 DLIEHPGVWQKIGQLHLKFERWNTTLHSRPRLIKGYGGWISIKNLPLEFWCKQIFEAIGS 312
           +L+     W   G+ HL FE+W+   HSRP  IKG+ GW+SIKNLPL+ W + IFE IG+
Sbjct: 247 NLVVTQDKWFNYGKFHLLFEKWDVINHSRPSSIKGFRGWLSIKNLPLDLWRRAIFEVIGA 289

Query: 313 YFGGLEAIAIETLNLLNVTEARIQVKKNL 339
           +FGGLE+ A++TLNL+  T+ARIQV+KN+
Sbjct: 307 HFGGLESFALDTLNLIRCTDARIQVRKNV 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V8784.6e-3932.12DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5D3BI916.3e-3657.85Putative 3,4-dihydroxy-2-butanone kinase isoform X5 OS=Cucumis melo var. makuwa ... [more]
A0A5A7U1281.8e-3536.61Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3DVS92.7e-3151.56Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3E3A52.3e-3033.46Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold8466... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025558Domain of unknown function DUF4283PFAMPF14111DUF4283coord: 201..334
e-value: 1.6E-10
score: 40.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000538Bhi05M000538mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process
biological_process GO:0071704 organic substance metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:0016301 kinase activity