Bhi05G000515 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000515
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Phloem filament protein
Location	chr5: 20409093 .. 20409767 (+)
RNA-Seq Expression	Bhi05G000515
Synteny	Bhi05G000515

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGAAGAAGCACATCACCACAAAAACTTGCTTTCTCCAAATAATCTAAAGAGTGATAGCAAGATGAGTTTTCAGGAAAAGGAGGAGTGGTTACCAATCCCTGATGTAACCACACCTTGTGTGAAAGAAATTGTAAATTTTGTGGTGAATCAACACAACAAAGAAAGTGGAGATGATCTCGTCCTCAAAGGTGTTATCAAAGGATGGTTTAAGGAGTTGAGTGATGTGAAAACAAAGCATCGTTTGTATATTGAAGCAACAAACAACAAAGGAGTTGTACAACTGTATGAAGCAATTGTATGTGTCATCGAAAAAGGTGATAAACAAAGAGTGAGGACACTCCTATCTTTCCATGTCGGTTATTTGGATGAGAATGAACAATTTTGGATTGAAATTCCAAATGTTGAAGAATCTTGTGTGCAAGAAGTTGCAAAGTTTGCTGTGGCAGAGTATAATAAGCAAGCTGATGATAATCTCGTCTACATTTGTACGACTCAGGGTTGGTATCAGGAGGTGAATGCAGAGTATGGCCTATTGTTCGATCTTCGTATTAAGACGAAGGATTGTTTCGGACGTGTTCGTGAATATAAGGCTTTGGTTTCGGAGGACAGACAACCCAATGAGAAAATTCGAATACTCATATCTTTCAAACTTGTACCCAAGAAAAGTTGA

mRNA sequence

ATGGATGAAGAAGCACATCACCACAAAAACTTGCTTTCTCCAAATAATCTAAAGAGTGATAGCAAGATGAGTTTTCAGGAAAAGGAGGAGTGGTTACCAATCCCTGATGTAACCACACCTTGTGTGAAAGAAATTGTAAATTTTGTGGTGAATCAACACAACAAAGAAAGTGGAGATGATCTCGTCCTCAAAGGTGTTATCAAAGGATGGTTTAAGGAGTTGAGTGATGTGAAAACAAAGCATCGTTTGTATATTGAAGCAACAAACAACAAAGGAGTTGTACAACTGTATGAAGCAATTGTATGTGTCATCGAAAAAGGTGATAAACAAAGAGTGAGGACACTCCTATCTTTCCATGTCGGTTATTTGGATGAGAATGAACAATTTTGGATTGAAATTCCAAATGTTGAAGAATCTTGTGTGCAAGAAGTTGCAAAGTTTGCTGTGGCAGAGTATAATAAGCAAGCTGATGATAATCTCGTCTACATTTGTACGACTCAGGGTTGGTATCAGGAGGTGAATGCAGAGTATGGCCTATTGTTCGATCTTCGTATTAAGACGAAGGATTGTTTCGGACGTGTTCGTGAATATAAGGCTTTGGTTTCGGAGGACAGACAACCCAATGAGAAAATTCGAATACTCATATCTTTCAAACTTGTACCCAAGAAAAGTTGA

Coding sequence (CDS)

ATGGATGAAGAAGCACATCACCACAAAAACTTGCTTTCTCCAAATAATCTAAAGAGTGATAGCAAGATGAGTTTTCAGGAAAAGGAGGAGTGGTTACCAATCCCTGATGTAACCACACCTTGTGTGAAAGAAATTGTAAATTTTGTGGTGAATCAACACAACAAAGAAAGTGGAGATGATCTCGTCCTCAAAGGTGTTATCAAAGGATGGTTTAAGGAGTTGAGTGATGTGAAAACAAAGCATCGTTTGTATATTGAAGCAACAAACAACAAAGGAGTTGTACAACTGTATGAAGCAATTGTATGTGTCATCGAAAAAGGTGATAAACAAAGAGTGAGGACACTCCTATCTTTCCATGTCGGTTATTTGGATGAGAATGAACAATTTTGGATTGAAATTCCAAATGTTGAAGAATCTTGTGTGCAAGAAGTTGCAAAGTTTGCTGTGGCAGAGTATAATAAGCAAGCTGATGATAATCTCGTCTACATTTGTACGACTCAGGGTTGGTATCAGGAGGTGAATGCAGAGTATGGCCTATTGTTCGATCTTCGTATTAAGACGAAGGATTGTTTCGGACGTGTTCGTGAATATAAGGCTTTGGTTTCGGAGGACAGACAACCCAATGAGAAAATTCGAATACTCATATCTTTCAAACTTGTACCCAAGAAAAGTTGA

Protein sequence

MDEEAHHHKNLLSPNNLKSDSKMSFQEKEEWLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHRLYIEATNNKGVVQLYEAIVCVIEKGDKQRVRTLLSFHVGYLDENEQFWIEIPNVEESCVQEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKDCFGRVREYKALVSEDRQPNEKIRILISFKLVPKKS

Homology

[Close]

BLAST of Bhi05G000515 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.5e-07
Identity = 27/71 (38.03%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 133 IPNVEESCVQEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKDCFG 192
           I NV +  V  VAK+A+ E+NK++ + LV++   +G  Q V+   G  +DL+I  KD  G
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS---GTKYDLKIAAKDGGG 94

Query: 193 RVREYKALVSE 204
           +++ Y+A+V E
Sbjct: 95  KIKNYEAVVVE 102

[Close]

BLAST of Bhi05G000515 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-05
Identity = 28/71 (39.44%), Postives = 40/71 (56.34%), Query Frame = 0


Query: 31  WLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHRLYIEATNN 90
           W PI +VT P V EI  F V+++NK S   L  + V+ G  + +S   T +RL + A + 
Sbjct: 31  WSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSG--TNYRLKVAANDG 90

Query: 91  KGVVQLYEAIV 102
            GV + Y AIV
Sbjct: 91  DGVSKNYLAIV 99

[Close]

BLAST of Bhi05G000515 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 3.5e-06
Identity = 27/71 (38.03%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 133 IPNVEESCVQEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKDCFG 192
           I NV +  V  VAK+A+ E+NK++ + LV++   +G  Q V+   G  +DL+I  KD  G
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS---GTKYDLKIAAKDGGG 94

Query: 193 RVREYKALVSE 204
           +++ Y+A+V E
Sbjct: 95  KIKNYEAVVVE 102

[Close]

BLAST of Bhi05G000515 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 1.9e-04
Identity = 36/91 (39.56%), Postives = 51/91 (56.04%), Query Frame = 0


Query: 130 WIEIPNVEESCVQEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKD 189
           W  I N+  + VQ+VA+FAV+E+NKQA+D L Y    +G+ Q V    G  + L I  KD
Sbjct: 32  WRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVV---AGTNYRLVIAAKD 91

Query: 190 CFGRVREYKALVSEDRQPNEKIRILISFKLV 221
               V  Y+A+V +  +P    R L SF+ V
Sbjct: 92  -GAVVGNYEAVVWD--KPWMHFRNLTSFRKV 116

[Close]

BLAST of Bhi05G000515 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 1.9e-04
Identity = 28/71 (39.44%), Postives = 40/71 (56.34%), Query Frame = 0


Query: 31  WLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHRLYIEATNN 90
           W PI +VT P V EI  F V+++NK S   L  + V+ G  + +S   T +RL + A + 
Sbjct: 31  WSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSG--TNYRLKVAANDG 90

Query: 91  KGVVQLYEAIV 102
            GV + Y AIV
Sbjct: 91  DGVSKNYLAIV 99

[Close]

BLAST of Bhi05G000515 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 7.3e-04
Identity = 35/91 (38.46%), Postives = 51/91 (56.04%), Query Frame = 0


Query: 130 WIEIPNVEESCVQEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKD 189
           W  I ++  + VQ+VA+FAV+E+NKQA+D L Y    +G+ Q V    G  + L I  KD
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVV---AGTNYRLVIAAKD 91

Query: 190 CFGRVREYKALVSEDRQPNEKIRILISFKLV 221
               V  Y+A+V +  +P    R L SF+ V
Sbjct: 92  -GAVVGNYEAVVWD--KPWMHFRNLTSFRKV 116

[Close]

BLAST of Bhi05G000515 vs. ExPASy TrEMBL
Match: A0A5A7TIS8 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1814G00070 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 3.8e-64
Identity = 122/201 (60.70%), Postives = 155/201 (77.11%), Query Frame = 0


Query: 23  MSFQEKEEWLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHR 82
           MS  EK EW+ IPDV+TPCV +I  F VNQHN ESG+ L LK V+KGWF ELS  + KHR
Sbjct: 1   MSCLEKGEWIKIPDVSTPCVHDIATFAVNQHNNESGEKLTLKSVVKGWFLELSYDRLKHR 60

Query: 83  LYIEATNNKGVVQLYEAIVCVIEKGDKQRVRTLLSFHVGYLDEN-EQFWIEIPNVEESCV 142
           L + ATN+KGVV+ YEAIVCVIEK   QRVR LLSF+ GYLDEN + FWIEI ++ + CV
Sbjct: 61  LILTATNDKGVVKTYEAIVCVIEKSGFQRVRKLLSFNAGYLDENGDLFWIEILDINQPCV 120

Query: 143 QEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKDCFGRVREYKALV 202
           Q+VAK AVA++N++  D+LVYI  TQGWY+E++  Y +LF++ + TKDCFGRVRE+KALV
Sbjct: 121 QDVAKAAVAQHNEKEHDSLVYISITQGWYRELDVNYAILFNIHLITKDCFGRVREFKALV 180

Query: 203 SEDRQPNEKIRILISFKLVPK 223
            ED+   +KIR L SF+++ K
Sbjct: 181 LEDKHGKDKIRTLKSFEVIKK 201

[Close]

BLAST of Bhi05G000515 vs. ExPASy TrEMBL
Match: A0A0A0LYF4 (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_1G710160 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 2.0e-60
Identity = 116/200 (58.00%), Postives = 148/200 (74.00%), Query Frame = 0


Query: 23  MSFQEKEEWLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHR 82
           MS  +K EW  IP  TTPCV +I NF V +HNKE+G+ L LK VIKGWF EL DVK K R
Sbjct: 1   MSCVDKGEWSKIPVCTTPCVLDIANFAVTEHNKETGEKLTLKSVIKGWFLELGDVKLKFR 60

Query: 83  LYIEATNNKGVVQLYEAIVCVIEKGDKQRVRTLLSFHVGYLDENEQFWIEIPNVEESCVQ 142
           LYI A N+KGVV  YEA+VCV E GD +RV+ LLSF+VGYLDE + FWI I + + SCVQ
Sbjct: 61  LYILAANDKGVVLTYEAVVCVTEIGDFKRVKKLLSFNVGYLDEKDVFWIVIVDTKASCVQ 120

Query: 143 EVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKDCFGRVREYKALVS 202
           +VAKFAVA++N+   D+LVY+  T+GWY+E++    + F++ + TKDCFGRVRE+KALV 
Sbjct: 121 DVAKFAVAKHNEDEHDSLVYLSITKGWYRELDPYNAIFFEIHLTTKDCFGRVREFKALVL 180

Query: 203 EDRQPNEKIRILISFKLVPK 223
           ED+   EKIR L  F+++ K
Sbjct: 181 EDKPQKEKIRTLKYFEVIKK 200

[Close]

BLAST of Bhi05G000515 vs. ExPASy TrEMBL
Match: A0A0A0K3W6 (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G222880 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 6.4e-51
Identity = 110/204 (53.92%), Postives = 139/204 (68.14%), Query Frame = 0


Query: 23  MSFQEKEEWLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHR 82
           MS   K+ W+ IPD TTPCV E+    VNQHNK SGD LV K VI GWF E+ D   KHR
Sbjct: 1   MSCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHR 60

Query: 83  LYIEATNNKGVVQLYEAIVCVIEKGDKQRVRTLLSFHVGYLDENEQ--FWIEIPNVEESC 142
           L IE   +KGVV +YE I+ V++K D +RVRTL+SF  GYLDE     FWI+IP+V++SC
Sbjct: 61  LIIEVVTSKGVVLIYELIIVVVQK-DGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSC 120

Query: 143 VQEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKDCFGRVREYKAL 202
           VQ+ AK+AV E+NK   D L Y  T +GWY EVN  + ++F L IK K+C GRVR+Y A+
Sbjct: 121 VQDCAKWAVTEHNKITHDYLAYDSTVEGWYHEVN-PFVIIFKLHIKAKECIGRVRDYIAV 180

Query: 203 VSEDRQPNEKIRILISFKLV-PKK 224
           V E++   EKI+ L  F L+ PKK
Sbjct: 181 VKEEKPLTEKIKKLEDFYLIRPKK 202

[Close]

BLAST of Bhi05G000515 vs. ExPASy TrEMBL
Match: A0A5D3D2J2 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G001660 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 3.5e-49
Identity = 104/204 (50.98%), Postives = 136/204 (66.67%), Query Frame = 0


Query: 23  MSFQEKEEWLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHR 82
           MS   K+EW+ IPD  TPCV E+    VNQHNK SGD+LV K VI GWF E+ D   KHR
Sbjct: 1   MSCGSKKEWIKIPDCNTPCVIEVAKDAVNQHNKASGDNLVFKRVIIGWFLEIDDHTIKHR 60

Query: 83  LYIEATNNKGVVQLYEAIVCVIEKGDKQRVRTLLSFHVGYLDENEQ--FWIEIPNVEESC 142
           LYIE  N+KGVV +   ++ V+ K D +RVRTL+S H GY DE +   FW +IP+V + C
Sbjct: 61  LYIEVINSKGVVLIISIVIVVVTK-DGKRVRTLVSSHHGYPDEKDLFIFWNKIPDVNQPC 120

Query: 143 VQEVAKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYG-LLFDLRIKTKDCFGRVREYKA 202
           VQ VAK+AV ++N    D+LVY+ T +GWY E     G L++ L IK K+C GRVR+Y A
Sbjct: 121 VQNVAKWAVNKHNTDTHDHLVYVSTVEGWYIEAADHSGTLVYKLHIKAKECIGRVRDYCA 180

Query: 203 LVSEDRQPNEKIRILISFKLVPKK 224
           +V E++   E+IR L+ F L+P K
Sbjct: 181 VVKEEKPLTERIRTLLEFFLIPPK 203

[Close]

BLAST of Bhi05G000515 vs. ExPASy TrEMBL
Match: A0A1S3BHQ3 (uncharacterized protein LOC103489701 OS=Cucumis melo OX=3656 GN=LOC103489701 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 4.1e-42
Identity = 94/198 (47.47%), Postives = 126/198 (63.64%), Query Frame = 0


Query: 27  EKEEWLPIPDVTTPCVKEIVNFVVNQHNKESGDDLVLKGVIKGWFKELSDVKTKHRLYIE 86
           +KE+W+ I D TTPCV       V+ HNKE GD+LV K VI GW+ E+   KTK RL IE
Sbjct: 6   KKEQWIQIHDTTTPCVINFGKHAVDDHNKEKGDNLVFKKVIIGWYLEIDYNKTKLRLIIE 65

Query: 87  ATNNKGVVQLYEAIVCVIEKGDKQRVRTLLSFHVGYLDENE--QFWIEIPNVEESCVQEV 146
             N+KG V  +E ++ V +K D + V+T++SF  G++DE     FWI+I NV E  VQE+
Sbjct: 66  VVNSKGEVITFEVVIVVDDK-DGKTVKTVISFVPGHIDEKNPWTFWIKIHNVTEPHVQEI 125

Query: 147 AKFAVAEYNKQADDNLVYICTTQGWYQEVNAEYGLLFDLRIKTKDCFGRVREYKALVSED 206
           AKFAV E+NK   DNL+YIC  +GWY         L+ L +K KDC GRVREY+ALV E+
Sbjct: 126 AKFAVEEHNKITHDNLIYICIIEGWYHVSTIPPVTLWKLHLKAKDCLGRVREYEALVKEE 185

Query: 207 RQPNEKIRILISFKLVPK 223
           +   + +  L  F ++PK
Sbjct: 186 KLLPKHVLKLEHFIVIPK 202

The following BLAST results are available for this feature:

BLAST of Bhi05G000515 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16500.1	2.5e-07	38.03	Cystatin/monellin superfamily protein	[more]
AT5G47550.1	1.4e-05	39.44	Cystatin/monellin superfamily protein	[more]

BLAST of Bhi05G000515 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WT8	3.5e-06	38.03	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]
P86472	1.9e-04	39.56	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q41916	1.9e-04	39.44	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
Q6TPK4	7.3e-04	38.46	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]

BLAST of Bhi05G000515 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TIS8	3.8e-64	60.70	Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A0A0LYF4	2.0e-60	58.00	Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_1G710160 PE=4 SV=1	[more]
A0A0A0K3W6	6.4e-51	53.92	Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G222880 PE=4 SV=1	[more]
A0A5D3D2J2	3.5e-49	50.98	Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3BHQ3	4.1e-42	47.47	uncharacterized protein LOC103489701 OS=Cucumis melo OX=3656 GN=LOC103489701 PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 26..120 e-value: 6.1E-14 score: 54.0
None	No IPR available	GENE3D	3.10.450.10		coord: 130..224 e-value: 3.5E-13 score: 51.4
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 27..119 coord: 127..222
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 30..109
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 130..221
IPR009994	Phloem filament PP1	PFAM	PF07430	PP1	coord: 40..118 e-value: 6.6E-13 score: 48.5 coord: 140..217 e-value: 4.8E-17 score: 61.8
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 27..119
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 127..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000515	Bhi05M000515	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity