Bhi05G000509 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000509
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPhloem filament protein
Locationchr5: 20156371 .. 20157259 (+)
RNA-Seq ExpressionBhi05G000509
SyntenyBhi05G000509
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGGAAGAAGCATCACCACACAAAACTTACTTTCTCTAAATACTAAAGATCATTTATTAAAGAGAGATAGCAAGATGGTTTGTCCAGACAAGAACGTGTGGTACCCATTGCCTGATCTTTCTTTAGATTGTGTGCAAGAAATTGCGAAATTTGCAGTGAAAAAAGGCAGTGACCCAAACAACCCGCTGAATTTGAAGAAAATTACAGACGGATGGTATTTGGAGTTGGATGATGACAGAGTAATCTATCGTTTACGTATCATAGCAACCAACGAGAAAGGAGAAGAAGGGCTATATCAAACAGAGGTTCTTGATGAGTATGTAGATGAGAAAAAAAGACAGTCACTCATTTATTTCCGTCCTGGGGTTATACTTGACGGTGAATTCTGGTTTATAGTTCCTGATGTTGACGATTCTCGTATCCAAGAGATTGCGAAGTTTGCAGTGGCTGAGCATAACAAGAAGGCTAATGATAATCTCGTATACATTTCAACTACTCGCGGCTACTATTTTGATGTGGATGAAATTACTAGAAGGTATAAACTTGTTCTCAAGGCGAAGGATTGTTTGGGACGTGTGCGGGACTATGATGCTTTGGTGCTTGAAGAGAAAACGGATTCTGGGACGACTTTAAAATTTGAATCTTTAAAGATTATCCCATGGAAATGTTGAAAAGACTTGCTATCTAAGTGAGTTTTGTTATGCTATGTGTACGTCCTTGACTATAACTATATATAAATAGTCAAGTGCTTAATAAATAAAGCATTCTAAAATATATCATGCTCTATGACTATGTTTTGTTTTGTTTTGTTTTTTTTTGAAACCGAAATATCGATTGATCGAGCTAAACTATGAGATTAATGAAAACGTAAAAATGTAGAAGTTTTCG

mRNA sequence

AAGGGAAGAAGCATCACCACACAAAACTTACTTTCTCTAAATACTAAAGATCATTTATTAAAGAGAGATAGCAAGATGGTTTGTCCAGACAAGAACGTGTGGTACCCATTGCCTGATCTTTCTTTAGATTGTGTGCAAGAAATTGCGAAATTTGCAGTGAAAAAAGGCAGTGACCCAAACAACCCGCTGAATTTGAAGAAAATTACAGACGGATGGTATTTGGAGTTGGATGATGACAGAGTAATCTATCGTTTACGTATCATAGCAACCAACGAGAAAGGAGAAGAAGGGCTATATCAAACAGAGGTTCTTGATGAGTATGTAGATGAGAAAAAAAGACAGTCACTCATTTATTTCCGTCCTGGGGTTATACTTGACGGTGAATTCTGGTTTATAGTTCCTGATGTTGACGATTCTCGTATCCAAGAGATTGCGAAGTTTGCAGTGGCTGAGCATAACAAGAAGGCTAATGATAATCTCGTATACATTTCAACTACTCGCGGCTACTATTTTGATGTGGATGAAATTACTAGAAGGTATAAACTTGTTCTCAAGGCGAAGGATTGTTTGGGACGTGTGCGGGACTATGATGCTTTGGTGCTTGAAGAGAAAACGGATTCTGGGACGACTTTAAAATTTGAATCTTTAAAGATTATCCCATGGAAATGTTGAAAAGACTTGCTATCTAAGTGAGTTTTGTTATGCTATGTGTACGTCCTTGACTATAACTATATATAAATAGTCAAGTGCTTAATAAATAAAGCATTCTAAAATATATCATGCTCTATGACTATGTTTTGTTTTGTTTTGTTTTTTTTTGAAACCGAAATATCGATTGATCGAGCTAAACTATGAGATTAATGAAAACGTAAAAATGTAGAAGTTTTCG

Coding sequence (CDS)

AAGGGAAGAAGCATCACCACACAAAACTTACTTTCTCTAAATACTAAAGATCATTTATTAAAGAGAGATAGCAAGATGGTTTGTCCAGACAAGAACGTGTGGTACCCATTGCCTGATCTTTCTTTAGATTGTGTGCAAGAAATTGCGAAATTTGCAGTGAAAAAAGGCAGTGACCCAAACAACCCGCTGAATTTGAAGAAAATTACAGACGGATGGTATTTGGAGTTGGATGATGACAGAGTAATCTATCGTTTACGTATCATAGCAACCAACGAGAAAGGAGAAGAAGGGCTATATCAAACAGAGGTTCTTGATGAGTATGTAGATGAGAAAAAAAGACAGTCACTCATTTATTTCCGTCCTGGGGTTATACTTGACGGTGAATTCTGGTTTATAGTTCCTGATGTTGACGATTCTCGTATCCAAGAGATTGCGAAGTTTGCAGTGGCTGAGCATAACAAGAAGGCTAATGATAATCTCGTATACATTTCAACTACTCGCGGCTACTATTTTGATGTGGATGAAATTACTAGAAGGTATAAACTTGTTCTCAAGGCGAAGGATTGTTTGGGACGTGTGCGGGACTATGATGCTTTGGTGCTTGAAGAGAAAACGGATTCTGGGACGACTTTAAAATTTGAATCTTTAAAGATTATCCCATGGAAATGTTGA

Protein sequence

KGRSITTQNLLSLNTKDHLLKRDSKMVCPDKNVWYPLPDLSLDCVQEIAKFAVKKGSDPNNPLNLKKITDGWYLELDDDRVIYRLRIIATNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGEFWFIVPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRDYDALVLEEKTDSGTTLKFESLKIIPWKC
Homology
BLAST of Bhi05G000509 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 3.6e-06
Identity = 23/71 (32.39%), Postives = 44/71 (61.97%), Query Frame = 0

Query: 133 VPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGR 192
           + +V D  +  +AK+A+ EHNK++ + LV++    G    V     +Y L + AKD  G+
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS--GTKYDLKIAAKDGGGK 94

Query: 193 VRDYDALVLEE 204
           +++Y+A+V+E+
Sbjct: 95  IKNYEAVVVEK 103

BLAST of Bhi05G000509 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-06
Identity = 30/74 (40.54%), Postives = 48/74 (64.86%), Query Frame = 0

Query: 130 WFIVPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDC 189
           W  + +++ + +Q++A+FAV+EHNK+AND L Y S  RGY   V      Y+LV+ AKD 
Sbjct: 32  WRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVV--AGTNYRLVIAAKDG 91

Query: 190 LGRVRDYDALVLEE 204
              V +Y+A+V ++
Sbjct: 92  -AVVGNYEAVVWDK 102

BLAST of Bhi05G000509 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.1e-06
Identity = 30/74 (40.54%), Postives = 47/74 (63.51%), Query Frame = 0

Query: 130 WFIVPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDC 189
           W  +  ++ + +Q++A+FAV+EHNK+AND L Y S  RGY   V      Y+LV+ AKD 
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVV--AGTNYRLVIAAKDG 91

Query: 190 LGRVRDYDALVLEE 204
              V +Y+A+V ++
Sbjct: 92  -AVVGNYEAVVWDK 102

BLAST of Bhi05G000509 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 5.1e-05
Identity = 23/71 (32.39%), Postives = 44/71 (61.97%), Query Frame = 0

Query: 133 VPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGR 192
           + +V D  +  +AK+A+ EHNK++ + LV++    G    V     +Y L + AKD  G+
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS--GTKYDLKIAAKDGGGK 94

Query: 193 VRDYDALVLEE 204
           +++Y+A+V+E+
Sbjct: 95  IKNYEAVVVEK 103

BLAST of Bhi05G000509 vs. ExPASy TrEMBL
Match: A0A0A0K3W6 (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G222880 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.8e-29
Identity = 75/198 (37.88%), Postives = 112/198 (56.57%), Query Frame = 0

Query: 26  MVCPDKNVWYPLPDLSLDCVQEIAKFAVKKGSDPN-NPLNLKKITDGWYLELDDDRVIYR 85
           M C  K  W  +PD +  CV E+AK  V + +  + + L  K++  GW+ E+DD  + +R
Sbjct: 1   MSCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHR 60

Query: 86  LRIIATNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRI 145
           L I     KG   +Y+  ++    D K+ ++LI F PG + +     FW  +PDV  S +
Sbjct: 61  LIIEVVTSKGVVLIYELIIVVVQKDGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSCV 120

Query: 146 QEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRDYDALVL 205
           Q+ AK+AV EHNK  +D L Y ST  G+Y +V+     +KL +KAK+C+GRVRDY A+V 
Sbjct: 121 QDCAKWAVTEHNKITHDYLAYDSTVEGWYHEVNPFVIIFKLHIKAKECIGRVRDYIAVVK 180

Query: 206 EEKTDSGTTLKFESLKII 220
           EEK  +    K E   +I
Sbjct: 181 EEKPLTEKIKKLEDFYLI 198

BLAST of Bhi05G000509 vs. ExPASy TrEMBL
Match: A0A0A0LYF4 (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_1G710160 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 2.3e-29
Identity = 79/199 (39.70%), Postives = 121/199 (60.80%), Query Frame = 0

Query: 26  MVCPDKNVWYPLPDLSLDCVQEIAKFAV-KKGSDPNNPLNLKKITDGWYLELDDDRVIYR 85
           M C DK  W  +P  +  CV +IA FAV +   +    L LK +  GW+LEL D ++ +R
Sbjct: 1   MSCVDKGEWSKIPVCTTPCVLDIANFAVTEHNKETGEKLTLKSVIKGWFLELGDVKLKFR 60

Query: 86  LRIIATNEKGEEGLYQTEV-LDEYVDEKKRQSLIYFRPGVILDGE-FWFIVPDVDDSRIQ 145
           L I+A N+KG    Y+  V + E  D K+ + L+ F  G + + + FW ++ D   S +Q
Sbjct: 61  LYILAANDKGVVLTYEAVVCVTEIGDFKRVKKLLSFNVGYLDEKDVFWIVIVDTKASCVQ 120

Query: 146 EIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITR-RYKLVLKAKDCLGRVRDYDALVL 205
           ++AKFAVA+HN+  +D+LVY+S T+G+Y ++D      +++ L  KDC GRVR++ ALVL
Sbjct: 121 DVAKFAVAKHNEDEHDSLVYLSITKGWYRELDPYNAIFFEIHLTTKDCFGRVREFKALVL 180

Query: 206 EEK--TDSGTTLK-FESLK 218
           E+K   +   TLK FE +K
Sbjct: 181 EDKPQKEKIRTLKYFEVIK 199

BLAST of Bhi05G000509 vs. ExPASy TrEMBL
Match: A0A5A7TIS8 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1814G00070 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 4.0e-29
Identity = 80/200 (40.00%), Postives = 121/200 (60.50%), Query Frame = 0

Query: 26  MVCPDKNVWYPLPDLSLDCVQEIAKFAVKK-GSDPNNPLNLKKITDGWYLELDDDRVIYR 85
           M C +K  W  +PD+S  CV +IA FAV +  ++    L LK +  GW+LEL  DR+ +R
Sbjct: 1   MSCLEKGEWIKIPDVSTPCVHDIATFAVNQHNNESGEKLTLKSVVKGWFLELSYDRLKHR 60

Query: 86  LRIIATNEKGEEGLYQTEV-LDEYVDEKKRQSLIYFRPGVILDGE--FWFIVPDVDDSRI 145
           L + ATN+KG    Y+  V + E    ++ + L+ F  G + +    FW  + D++   +
Sbjct: 61  LILTATNDKGVVKTYEAIVCVIEKSGFQRVRKLLSFNAGYLDENGDLFWIEILDINQPCV 120

Query: 146 QEIAKFAVAEHNKKANDNLVYISTTRGYYFDVD-EITRRYKLVLKAKDCLGRVRDYDALV 205
           Q++AK AVA+HN+K +D+LVYIS T+G+Y ++D      + + L  KDC GRVR++ ALV
Sbjct: 121 QDVAKAAVAQHNEKEHDSLVYISITQGWYRELDVNYAILFNIHLITKDCFGRVREFKALV 180

Query: 206 LEEK--TDSGTTLK-FESLK 218
           LE+K   D   TLK FE +K
Sbjct: 181 LEDKHGKDKIRTLKSFEVIK 200

BLAST of Bhi05G000509 vs. ExPASy TrEMBL
Match: A0A1S3BHQ3 (uncharacterized protein LOC103489701 OS=Cucumis melo OX=3656 GN=LOC103489701 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 9.8e-28
Identity = 80/199 (40.20%), Postives = 110/199 (55.28%), Query Frame = 0

Query: 31  KNVWYPLPDLSLDCVQEIAKFAV-KKGSDPNNPLNLKKITDGWYLELDDDRVIYRLRIIA 90
           K  W  + D +  CV    K AV     +  + L  KK+  GWYLE+D ++   RL I  
Sbjct: 7   KEQWIQIHDTTTPCVINFGKHAVDDHNKEKGDNLVFKKVIIGWYLEIDYNKTKLRLIIEV 66

Query: 91  TNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRIQEIAK 150
            N KGE   ++  ++ +  D K  +++I F PG I +     FW  + +V +  +QEIAK
Sbjct: 67  VNSKGEVITFEVVIVVDDKDGKTVKTVISFVPGHIDEKNPWTFWIKIHNVTEPHVQEIAK 126

Query: 151 FAVAEHNKKANDNLVYISTTRGYYF--DVDEITRRYKLVLKAKDCLGRVRDYDALVLEEK 210
           FAV EHNK  +DNL+YI    G+Y    +  +T  +KL LKAKDCLGRVR+Y+ALV EEK
Sbjct: 127 FAVEEHNKITHDNLIYICIIEGWYHVSTIPPVT-LWKLHLKAKDCLGRVREYEALVKEEK 186

Query: 211 TDSGTTLKFESLKIIPWKC 224
                 LK E   +IP  C
Sbjct: 187 LLPKHVLKLEHFIVIPKIC 204

BLAST of Bhi05G000509 vs. ExPASy TrEMBL
Match: A0A5A7TFI4 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G001740 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.9e-26
Identity = 79/199 (39.70%), Postives = 108/199 (54.27%), Query Frame = 0

Query: 31  KNVWYPLPDLSLDCVQEIAKFAV-KKGSDPNNPLNLKKITDGWYLELDDDRVIYRLRIIA 90
           K  W  + D +  CV    K AV     +  + L  KK+  GWYLE+D ++   RL I  
Sbjct: 7   KEQWIQIHDTTTPCVINFGKHAVDDHNKEKGDNLVFKKVIIGWYLEIDYNKTKLRLIIEV 66

Query: 91  TNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRIQEIAK 150
            N KGE   ++  ++ +  D K  +++I F PG I +     FW  + +V +  +QEIAK
Sbjct: 67  VNSKGEVITFEVVIVVDDKDGKTVKTVISFVPGHIDEKNPWTFWIKIHNVTEPHVQEIAK 126

Query: 151 FAVAEHNKKANDNLVYISTTRG-YYFDVDEITRRYKLVLKAKDCLGRVRDYDALVLEEK- 210
           FAV EHNK  +DNL+YI    G Y+     +   +KL LKAKDCLGRVR+Y+ALV EEK 
Sbjct: 127 FAVEEHNKITHDNLIYICIIEGWYHVPTIPLVTLWKLHLKAKDCLGRVREYEALVKEEKL 186

Query: 211 TDSGTTLKFESLKIIPWKC 224
                 LK E   +IP  C
Sbjct: 187 LLPKHVLKLEHFIVIPKIC 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G16500.13.6e-0632.39Cystatin/monellin superfamily protein [more]
Match NameE-valueIdentityDescription
P864721.6e-0640.54Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841... [more]
Q6TPK42.1e-0640.54Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q84WT85.1e-0532.39Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0K3W61.8e-2937.88Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G222880 PE=4 SV=1[more]
A0A0A0LYF42.3e-2939.70Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_1G710160 PE=4 SV=1[more]
A0A5A7TIS84.0e-2940.00Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3BHQ39.8e-2840.20uncharacterized protein LOC103489701 OS=Cucumis melo OX=3656 GN=LOC103489701 PE=... [more]
A0A5A7TFI41.9e-2639.70Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 134..209
e-value: 8.9E-17
score: 61.2
IPR000010Cystatin domainCDDcd00042CYcoord: 132..221
e-value: 2.25958E-5
score: 40.15
IPR009994Phloem filament PP1PFAMPF07430PP1coord: 44..112
e-value: 2.9E-7
score: 30.4
NoneNo IPR availableGENE3D3.10.450.10coord: 127..217
e-value: 4.1E-13
score: 51.3
coord: 31..121
e-value: 2.6E-7
score: 32.7
NoneNo IPR availablePANTHERPTHR47116:SF5PHLOEM FILAMENT PROTEINcoord: 31..120
NoneNo IPR availablePANTHERPTHR47116:SF5PHLOEM FILAMENT PROTEINcoord: 130..218
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 131..207
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 43..112
IPR027214CystatinPANTHERPTHR47116PHLOEM FILAMENT PROTEINcoord: 130..218
IPR027214CystatinPANTHERPTHR47116PHLOEM FILAMENT PROTEINcoord: 31..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000509Bhi05M000509mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity