Bhi05G000455 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000455
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	membrane steroid-binding protein 2
Location	chr5: 17325176 .. 17327857 (-)
RNA-Seq Expression	Bhi05G000455
Synteny	Bhi05G000455

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTAAATGTTGTCCACGGACAAGGACAGCCGTTGATTAAAGAGCTTTTAGGTTGGTAGCAAAAAGCTGCTTCCCTTTTCCTTCTCTCACCCTCCTTCCTCTCTCTTTCTCTCTGTCTTCTCTCTATCCGCCATGGCTCTGCAAGTCTGGGAGACGCTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCCGCCACCTTCTTCACAGTCTTGGCTCTCGGTATTGCCATTTACTATCTCATTTCAAGTTTCTTCGCTCCCTCCGACTACGGAACCCACACTAGGGACTTGGGTGAGCAACTACAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAAATTTCTGAGGATGACTTGAAGCAATACGATGGCTCCGATCCCAAGAAGCCTCTTCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGGTCCGTTGAGGGTTTTCTTTCTTTCGAATTTTTTCACTCTGTTTTAGGTATTTTGTGTTTCGATGATCGTGATAAGTTTTGTTTCGGTTTCAATTTGTTTTTGAGTCAACGTGTTTGTTCAGCTCTTTGATGGCATGTGGAACATGAATATCAAGCTGATTTTAGGTTTACAGAAGATTTAATTGATTTTGGTGCTTCGTTTTGCCATCTATAGTTCTTCTTTTCGTTCAAATAGCTACTGATTCCTTCCAAATGAAACTTAATTTGGTCCTACTGCATTGAACCGCTATTGATATTTTTTAGCCTATTTCTAATTCCCTGTTTCTTATACTAGGAGCCATGCATTGTCTGTTTGATGCTAGTTATAGTAAAAGAAGATTATTAATAGTTTCTATAAAAATTGTGCTTTCTGTTACGAGTTTATAAAACTAGGGGTGCGAGCTTAAAACGGAATTTACACTTTTTGGTTGATTTGGTTTACATGGGTCTTGCGGATTTCTGTGCTGACCTGCTTACAAGTTACACCCCTATCTTTAATGGTGTTCTATGCTTGTCATAATATATATGCTCCCACGACCAGATACTTCAATTCAAACTTTCAATCTCTTTAGTGGTGGATGATAAAACCTCTCTAAATATATTGGTGAGATCAAACTTTTTGTTATTTGTTCATCTGAATTTTAACTTCGATAGTTCAACATTTCTTCATTCCTTCTTATAGCTGCGATTTGCTATGTATCTAGTTTTGAAAATATTCTGTACTGACTGTCATTTTTTACTTTCAAATTACGGCCATGATGTTTCCAGCATATAGAATTCGTATAGTACTACTGTACATGTAGTTCACATAGGCAAGAGATTGGTGTTCCACAAGTTATTCTGAACTGCTATGTTTTAAGAGCTATTTTTTGTGGTGGCTTGTAGATAAACCAATCCTTCTTTTATTTGGGATCGGAAAAGATTCTGTTACATGTAAAATCATTTTTACAAATTACAAGCTCATGATTTCAGATCACTTTTTACAAGCGTTTGATTTGAAAGACAGAATAAGTTTAATAGAAAACAATGCAGAAACCTCCTCTCATGATGTGCTAAAAAGTAGATAGTTTCTTTTTGAAAAACATTATAAGAACAGGTCCCTTGTTTTCATGATTAGGTACACTCTTGGACATGCATTCAACTGTTTTAGAACATCTTTGGCTATCTGACGAGTTTTTCTGATTGTGTATTATTTACTACGCTAATGAAGCATGTTTGAGAGTGATTTTAAAATTATTAAAATCACTTTATCATTTTCTAAATTATTAGAAAAATGTTTTAAGCATTCCAAATTAATTTCAGTAGTACCAACATGCGATTAAAAGTGTAAAATTAGATATTGAAGTGAATTTGAATGATTAAAAGTGTGTTTCGAAGTGATTTTAAACATGAAAAATTAGACATGAAGAGAGTGATTTCACCATTTCAAAATCTCTCCTAAATATGCGTACTAAGGCAAGTCAAATATCCAATACTTTGCAGTGTCTTGCTCTCTTGTGTGTGCGTCCTCTTAAAGGAAAATTAAAATTGGAGAAATTGCACTTTGTATCCGTCAGGTTTTAGTCTTGTATACGGCCATAGATAGTGCTTCAATTTTCTATATTTCTCTTAATCAAGCTTTCATATTTCTTTGCGATTATCTCTTCTAAACGGTTCTACTTGCAGAATGTTCTATGGACCTGGGGGGCCCTATGCGCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCTTTTGAAGAAAAAGATCTGACTGGCGATATCTCCGGTCTCGGTCCATTCGAACTCGAGGCCTTGCAGGATTGGGAATATAAATTCATGAGTAAATATGTTAAAGTTGGGACAGTTCAGAAGTCAGTTGCTGATGGTGATGCTGCAGCTGGTGAGCCTGCAGAATCAAGCATTGCTGAAGTTAGTAAGCCTGCCGAAGAAAGTCACTCGGAATCTCGAGATGATAAAGTTGAGGAAACCCCTGCTGCTGCAAATGTCGATTAATATTCTTCTGTCTACAGATGCCCCTTTCGACGGATATAGGAAAAGAGAACAATGTATGTGTGCTCATGTTTCAGTAATCTTGTTTCATAGATAAGTGAAATCTAGTTGTGTAGGGTGATGATGTTACAGGTGGAGTTAAGGCTTGAGTTTATGTTTACTATTGTTCAATAAACATCTGACAATAAGATAATTAGTGTTTGAATTT

mRNA sequence

GTTAAATGTTGTCCACGGACAAGGACAGCCGTTGATTAAAGAGCTTTTAGGTTGGTAGCAAAAAGCTGCTTCCCTTTTCCTTCTCTCACCCTCCTTCCTCTCTCTTTCTCTCTGTCTTCTCTCTATCCGCCATGGCTCTGCAAGTCTGGGAGACGCTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCCGCCACCTTCTTCACAGTCTTGGCTCTCGGTATTGCCATTTACTATCTCATTTCAAGTTTCTTCGCTCCCTCCGACTACGGAACCCACACTAGGGACTTGGGTGAGCAACTACAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAAATTTCTGAGGATGACTTGAAGCAATACGATGGCTCCGATCCCAAGAAGCCTCTTCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGAATGTTCTATGGACCTGGGGGGCCCTATGCGCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCTTTTGAAGAAAAAGATCTGACTGGCGATATCTCCGGTCTCGGTCCATTCGAACTCGAGGCCTTGCAGGATTGGGAATATAAATTCATGAGTAAATATGTTAAAGTTGGGACAGTTCAGAAGTCAGTTGCTGATGGTGATGCTGCAGCTGGTGAGCCTGCAGAATCAAGCATTGCTGAAGTTAGTAAGCCTGCCGAAGAAAGTCACTCGGAATCTCGAGATGATAAAGTTGAGGAAACCCCTGCTGCTGCAAATGTCGATTAATATTCTTCTGTCTACAGATGCCCCTTTCGACGGATATAGGAAAAGAGAACAATGTATGTGTGCTCATGTTTCAGTAATCTTGTTTCATAGATAAGTGAAATCTAGTTGTGTAGGGTGATGATGTTACAGGTGGAGTTAAGGCTTGAGTTTATGTTTACTATTGTTCAATAAACATCTGACAATAAGATAATTAGTGTTTGAATTT

Coding sequence (CDS)

ATGGCTCTGCAAGTCTGGGAGACGCTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCCGCCACCTTCTTCACAGTCTTGGCTCTCGGTATTGCCATTTACTATCTCATTTCAAGTTTCTTCGCTCCCTCCGACTACGGAACCCACACTAGGGACTTGGGTGAGCAACTACAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAAATTTCTGAGGATGACTTGAAGCAATACGATGGCTCCGATCCCAAGAAGCCTCTTCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGAATGTTCTATGGACCTGGGGGGCCCTATGCGCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCTTTTGAAGAAAAAGATCTGACTGGCGATATCTCCGGTCTCGGTCCATTCGAACTCGAGGCCTTGCAGGATTGGGAATATAAATTCATGAGTAAATATGTTAAAGTTGGGACAGTTCAGAAGTCAGTTGCTGATGGTGATGCTGCAGCTGGTGAGCCTGCAGAATCAAGCATTGCTGAAGTTAGTAAGCCTGCCGAAGAAAGTCACTCGGAATCTCGAGATGATAAAGTTGAGGAAACCCCTGCTGCTGCAAATGTCGATTAA

Protein sequence

MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPSDYGTHTRDLGEQLQPLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEPAESSIAEVSKPAEESHSESRDDKVEETPAAANVD

Homology

[Close]

BLAST of Bhi05G000455 vs. TAIR 10
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3 )

HSP 1 Score: 264.6 bits (675), Expect = 6.8e-71
Identity = 142/224 (63.39%), Postives = 168/224 (75.00%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFF-APSDYGTHTRDLGEQLQ 60
           M  Q+WETLKE I AYTGLSPA FFTVLAL  A+Y ++S FF +P  +   + ++  Q +
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GEI+E++LK YDGSD KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEP 180
           SRALAKMSFE++DLTGDISGLG FELEALQDWEYKFMSKYVKVGT+QK   +G  ++ EP
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESS-EP 180

Query: 181 AESSIA-----------EVSKPAEESHSESRDDKVEETPAAANV 213
           +E+  A           E S    +  S S  +K+ ET    +V
Sbjct: 181 SEAKTASAEGLSTNTGEEASAITHDETSRSTGEKIAETTEKKDV 223

[Close]

BLAST of Bhi05G000455 vs. TAIR 10
Match: AT5G52240.1 (membrane steroid binding protein 1 )

HSP 1 Score: 253.4 bits (646), Expect = 1.6e-67
Identity = 140/218 (64.22%), Postives = 161/218 (73.85%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  AIY +IS +FA    D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGEI+E++LKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAA 180
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGTV+        A
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVK-------VA 180

Query: 181 AGEPAESSIAEVSKPAEESHSESRDDKVEETPAAANVD 214
             EP  +S++E ++  E+      D  V  TP    VD
Sbjct: 181 GSEPETASVSEPTENVEQ------DAHVTTTPGKTVVD 205

[Close]

BLAST of Bhi05G000455 vs. TAIR 10
Match: AT5G52240.2 (membrane steroid binding protein 1 )

HSP 1 Score: 252.7 bits (644), Expect = 2.7e-67
Identity = 128/171 (74.85%), Postives = 143/171 (83.63%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  AIY +IS +FA    D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGEI+E++LKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQ 167
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGTV+
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVK 171

[Close]

BLAST of Bhi05G000455 vs. TAIR 10
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2 )

HSP 1 Score: 108.6 bits (270), Expect = 6.2e-24
Identity = 49/97 (50.52%), Postives = 68/97 (70.10%), Query Frame = 0


Query: 69  EISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 128
           E + + L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 129 EEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTV 166
            E+D++  + GL   E+  L DWE KF +KY  VG V
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

[Close]

BLAST of Bhi05G000455 vs. TAIR 10
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4 )

HSP 1 Score: 73.6 bits (179), Expect = 2.2e-13
Identity = 43/140 (30.71%), Postives = 72/140 (51.43%), Query Frame = 0


Query: 71  SEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEE 130
           S ++L  Y+G+D   P+L+ I G ++DV++ +  YG GG Y  FAG+DASRA    +F  
Sbjct: 43  SAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTG 102

Query: 131 KDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTV-----------QKSVADGDAAAGEP 190
             LT  + GL   E++++ DW   +   Y  VG +            K +   +A A   
Sbjct: 103 DGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLVGRYYDSQGNPTKHLKGAEAKASRG 162

Query: 191 AESSIAEVSKPAEESHSESR 200
           A+    + ++ A++S+  SR
Sbjct: 163 AQLMEKQKTEEAKQSNCNSR 182

[Close]

BLAST of Bhi05G000455 vs. ExPASy Swiss-Prot
Match: Q9M2Z4 (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 9.5e-70
Identity = 142/224 (63.39%), Postives = 168/224 (75.00%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFF-APSDYGTHTRDLGEQLQ 60
           M  Q+WETLKE I AYTGLSPA FFTVLAL  A+Y ++S FF +P  +   + ++  Q +
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GEI+E++LK YDGSD KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEP 180
           SRALAKMSFE++DLTGDISGLG FELEALQDWEYKFMSKYVKVGT+QK   +G  ++ EP
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESS-EP 180

Query: 181 AESSIA-----------EVSKPAEESHSESRDDKVEETPAAANV 213
           +E+  A           E S    +  S S  +K+ ET    +V
Sbjct: 181 SEAKTASAEGLSTNTGEEASAITHDETSRSTGEKIAETTEKKDV 223

[Close]

BLAST of Bhi05G000455 vs. ExPASy Swiss-Prot
Match: Q9FVZ7 (Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP1 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.2e-67
Identity = 145/224 (64.73%), Postives = 168/224 (75.00%), Query Frame = 0


Query: 4   QVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFA--PSDYGTHTRDLGEQLQPL 63
           ++WETLK+AIVAYTGLSPA FFT +A   A+Y+++S  FA  P       RD  E  +PL
Sbjct: 7   ELWETLKQAIVAYTGLSPAAFFTAVAAAAALYHVVSGIFAGPPPPPPPRPRDEPE-AEPL 66

Query: 64  PPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR 123
           PPPVQ+GE+SE++L+QYDGSDPKKPLLMAIKGQIYDV+QSRMFYGPGGPYALFAGKDASR
Sbjct: 67  PPPVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDASR 126

Query: 124 ALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGD-------- 183
           ALAKMSFE +DLTGDISGLGPFEL+ALQDWEYKFM KYVKVGTV+K+V   D        
Sbjct: 127 ALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMGKYVKVGTVKKTVPVEDGAPSTSPE 186

Query: 184 ----AAAGEPAESSIAEVSKPAEESHSESRDDKVEETPAAANVD 214
               AAA EP E + A   KP E S  E ++   E+  AAA  D
Sbjct: 187 TTETAAAAEP-EKAPATEEKPREVSSEEVKEK--EDAVAAAAPD 226

[Close]

BLAST of Bhi05G000455 vs. ExPASy Swiss-Prot
Match: Q9XFM6 (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 253.4 bits (646), Expect = 2.2e-66
Identity = 140/218 (64.22%), Postives = 161/218 (73.85%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  AIY +IS +FA    D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGEI+E++LKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAA 180
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGTV+        A
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVK-------VA 180

Query: 181 AGEPAESSIAEVSKPAEESHSESRDDKVEETPAAANVD 214
             EP  +S++E ++  E+      D  V  TP    VD
Sbjct: 181 GSEPETASVSEPTENVEQ------DAHVTTTPGKTVVD 205

[Close]

BLAST of Bhi05G000455 vs. ExPASy Swiss-Prot
Match: Q9FVZ9 (Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP2 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 7.9e-48
Identity = 106/185 (57.30%), Postives = 125/185 (67.57%), Query Frame = 0


Query: 11  EAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPSDYGTHTR-DLGEQLQPLPPPVQVGE 70
           E   A    SP      +A    +Y + SS  AP       R +  E+  P P PVQVGE
Sbjct: 61  ELWAAARSASPVAVIAAVAGAAVVYKVGSSLLAPPPPPARPREEPSEEAPPPPEPVQVGE 120

Query: 71  ISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFE 130
           I+ ++L QYDGSDP+KPLLMAIKGQIYDVSQSR+FYGPGGPYALFAGKDASRALAKMSFE
Sbjct: 121 ITAEELLQYDGSDPEKPLLMAIKGQIYDVSQSRLFYGPGGPYALFAGKDASRALAKMSFE 180

Query: 131 EKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEPAESSIAEVSK 190
            +DLT DISGL   EL ALQDWEYKF SKYVKVGT++K + +     G+    +I E + 
Sbjct: 181 PQDLTDDISGLSLLELSALQDWEYKFSSKYVKVGTIKKVLVE---QGGDSTADAIEEAAV 240

Query: 191 PAEES 195
             E+S
Sbjct: 241 DGEDS 242

[Close]

BLAST of Bhi05G000455 vs. ExPASy Swiss-Prot
Match: Q9SK39 (Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.8e-23
Identity = 49/97 (50.52%), Postives = 68/97 (70.10%), Query Frame = 0


Query: 69  EISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 128
           E + + L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 129 EEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTV 166
            E+D++  + GL   E+  L DWE KF +KY  VG V
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

[Close]

BLAST of Bhi05G000455 vs. ExPASy TrEMBL
Match: A0A0A0LIG2 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G915110 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.7e-99
Identity = 190/213 (89.20%), Postives = 200/213 (93.90%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPSDYGTHTRDLGEQLQP 60
           MALQVW+TLKEAI+AYTGLSP+TFFTVLALG+AIYYLISSFFAPSDYGTH RDL +Q+ P
Sbjct: 1   MALQVWDTLKEAILAYTGLSPSTFFTVLALGLAIYYLISSFFAPSDYGTHPRDL-DQIHP 60

Query: 61  LPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GEISEDDLKQYDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEPA 180
           RALAKMSFEEKDLTGDISGLGP ELE LQDWEYKFMSKYVKVGTV+ SVA+GDAAAGEPA
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPSELEMLQDWEYKFMSKYVKVGTVKTSVAEGDAAAGEPA 180

Query: 181 ESSIAEVSKPAEESHSESRDDKVEETPAAANVD 214
           ESS AEVSKP EESHSE RDDK EE PAAAN D
Sbjct: 181 ESSTAEVSKPVEESHSEPRDDKAEEAPAAANAD 212

[Close]

BLAST of Bhi05G000455 vs. ExPASy TrEMBL
Match: A0A5A7TA90 (Membrane steroid-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6571G00110 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.6e-99
Identity = 193/214 (90.19%), Postives = 200/214 (93.46%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPS-DYGTHTRDLGEQLQ 60
           MALQVWETLKEAIVAYTGLSP+TFFTVLALG AIYYLISSFFAPS DYGTH RDL EQ+ 
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGFAIYYLISSFFAPSADYGTHARDL-EQIH 60

Query: 61  PLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GEISEDDLKQYDG+D KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEISEDDLKQYDGTDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEP 180
           SRALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGTV+K+VA+GDAAAGEP
Sbjct: 121 SRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKTVAEGDAAAGEP 180

Query: 181 AESSIAEVSKPAEESHSESRDDKVEETPAAANVD 214
           AESS AEVSKP EESHSE RDDK EE PAAANVD
Sbjct: 181 AESSSAEVSKPVEESHSEPRDDKAEEAPAAANVD 213

[Close]

BLAST of Bhi05G000455 vs. ExPASy TrEMBL
Match: A0A1S3CQ79 (membrane steroid-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103503452 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.6e-99
Identity = 193/214 (90.19%), Postives = 200/214 (93.46%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPS-DYGTHTRDLGEQLQ 60
           MALQVWETLKEAIVAYTGLSP+TFFTVLALG AIYYLISSFFAPS DYGTH RDL EQ+ 
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGFAIYYLISSFFAPSADYGTHARDL-EQIH 60

Query: 61  PLPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GEISEDDLKQYDG+D KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEISEDDLKQYDGTDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEP 180
           SRALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGTV+K+VA+GDAAAGEP
Sbjct: 121 SRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKTVAEGDAAAGEP 180

Query: 181 AESSIAEVSKPAEESHSESRDDKVEETPAAANVD 214
           AESS AEVSKP EESHSE RDDK EE PAAANVD
Sbjct: 181 AESSSAEVSKPVEESHSEPRDDKAEEAPAAANVD 213

[Close]

BLAST of Bhi05G000455 vs. ExPASy TrEMBL
Match: A0A6J1I394 (membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470596 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.0e-98
Identity = 192/215 (89.30%), Postives = 202/215 (93.95%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSP+TFFTVLALG+A YYLISSFF PSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SED+LKQYDGSDP+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQIGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDA--AAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGT++K VA+GDA  AAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSIAEV--SKPAEESHSESRDDK-VEETPAAA 211
           PAESS AEV  SKPAE+S SES  DK  EETPAAA
Sbjct: 181 PAESSTAEVSSSKPAEDSPSESHADKAAEETPAAA 215

[Close]

BLAST of Bhi05G000455 vs. ExPASy TrEMBL
Match: A0A6J1GMC2 (membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455271 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.6e-98
Identity = 193/217 (88.94%), Postives = 202/217 (93.09%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSP+TFFTVLALG+A YYLISSFF PSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGE+SED+LKQYDGSDP+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDA--AAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGT++K VA+GDA  AAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSIAEV----SKPAEESHSESRDDK-VEETPAAA 211
           PAESS AEV    SKPAE+S SES  DK  EETPAAA
Sbjct: 181 PAESSTAEVSSSSSKPAEDSPSESHADKAAEETPAAA 217

The following BLAST results are available for this feature:

BLAST of Bhi05G000455 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48890.1	6.8e-71	63.39	membrane-associated progesterone binding protein 3	[more]
AT5G52240.1	1.6e-67	64.22	membrane steroid binding protein 1	[more]
AT5G52240.2	2.7e-67	74.85	membrane steroid binding protein 1	[more]
AT2G24940.1	6.2e-24	50.52	membrane-associated progesterone binding protein 2	[more]
AT4G14965.1	2.2e-13	30.71	membrane-associated progesterone binding protein 4	[more]

BLAST of Bhi05G000455 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2Z4	9.5e-70	63.39	Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1...	[more]
Q9FVZ7	1.2e-67	64.73	Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9XFM6	2.2e-66	64.22	Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1...	[more]
Q9FVZ9	7.9e-48	57.30	Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9SK39	8.8e-23	50.52	Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 S...	[more]

BLAST of Bhi05G000455 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIG2	2.7e-99	89.20	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A5A7TA90	4.6e-99	90.19	Membrane steroid-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CQ79	4.6e-99	90.19	membrane steroid-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103503452 PE=4 ...	[more]
A0A6J1I394	5.0e-98	89.30	membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]
A0A6J1GMC2	6.6e-98	88.94	membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 70..166 e-value: 1.6E-23 score: 94.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 72..165 e-value: 2.8E-14 score: 53.0
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 68..166 e-value: 7.9E-37 score: 127.5
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 61..166
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 170..213
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 188..207
None	No IPR available	PANTHER	PTHR10281:SF90	STEROID-BINDING PROTEIN 3-RELATED	coord: 1..207
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 1..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000455	Bhi05M000455	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0019904	protein domain specific binding