Bhi05G000437 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000437
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionphospholipase A1-Ibeta2, chloroplastic
Locationchr5: 16027742 .. 16029803 (-)
RNA-Seq ExpressionBhi05G000437
SyntenyBhi05G000437
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAAATCTTTCAACTATATAAGTCTATATATGAATCTCCTCCTCCATTTTCATTCACTTTCATTTGCGTCTTCTAAAACATCATCGCTGCGTCCCACAGCGCCGCCGCCTCTCTCTCTCTCTCTAAAAACCATGCAAATAATAAGTTCTACAATCCCGGCTCAAAATCTCTCCCAATTTCAGGTCCGCCGGTCGAGTTTCAAATACCAAATGTCGCCGTTGAACCCCTCAGCGGCGCAGATTTCTTCCTTTAAACCCGCCACGTCGACGGTTGATGGAGCTCGCCGGCATCTGGCAAATCTGGACAAGTTGTTGAAGAAAACCGATCCGCCGGTCAAGGTCGACCCGGAACCGGTTCACAAGGACCCGAATAAGGGATCGGTGATGCCGATTAAAGGAAAGGGGCTTTTAGAAGGGCTGAACCTGGCCAGAATTTGGCCGGAGGTAAAAGCGGTGGAGGAAATGTCGCCGAGAAATCTCAACAGGCTGCAGAGGCTACTGTCGAAAACGATGGAGTATTCACCGAGAAACGCCCTCGGCCAGCGGTGGCGCGAGTACCACGGTTGCAACGATTGGTCCGGTTTACTCGACCCGCTCGATGAAAATCTCCGCCGTGAAGTCGTCCGGTACGGGGAGTTCGTACAAGCCGCATACCATGCCTTCCACTCAAACCCAGCCACGTCACCCAACGAGGCCCCACTCCCTCGCCACGTGGCACTTCCGGATAGATCTTACAAAGTCACAAAGAGTCTCTACGCCACGTCATCAGTCGGGCTACCCGGTTGGGTGGACGAGGTCGCTCCGGATCTCGGCTGGATGACGCAGCGGTCCAGTTGGGTCGGATACGTCGCCGTTTGCGACGATCGCCGTGAAATCGCACGAATGGGACGACGCGATATCGTCATCGCACTTCGTGGCACCGCCACCTGTCTGGAATGGGCCGAAAATGTTAGAGCTCAACTCATCAACATCCCCGCTGACGCTGACGCTGCCGCGGAAGACGGTGGCGATCCGAAAGTGGAGTGCGGATTTCTAAGCCTGTATAAAACTGCTGGAGCACACGTGAAGAGCTTATCAGAGTCGGTGGTGGAGGAAATCCGACGATTAACGGAGATGTACAAAGGAGAAAGGTTGAGCATCACAGTGACCGGACACAGCCTAGGCGCGGCATTGGCTGTCCTGGTAGCGGACGAGATCAGCGTTTGTTCACCGGAGGTTCCGCCAGTGGCAGTGTTCTCATTCGGCGGACCGCGCGTGGGGAACAAAATCTTCGCAAATCGAATAAAATCTCGAAACGTGAAGGTATTGCGGATTGTAAACTCACAAGACGTGATAACACAGGTACCGCCAGTGACATACTCCCACGTGGGAACCGAGCTCCGAGTGGAGACAAAGATGTCGCCGTTCTTGAAACCTAACGCCGACGTAGCTTGTTGCCATGATTTGGAAGCGTATTTACATCTGGTCGACGGATTCTTGTCGTCTAAATGTCCGTTCCGACCGAATGCGAAACGGAGTTTAGTGCGACTGGTGCAAGATCAGAGGGGGAACGTGAAGAAATTGTATATGAGGAAAGTGAAGGATTTGGGCTTCAATCCAGAGCTGCAGACGGCGGGGTGTTTGCCGAGTCCCTCTTGATTTCATCACAATTTTACTAAATCTGGGGAGTTTGGTTGAACATGGTTGGATTCTAGGCATTAGATTTCATGAACATACACAATTTCAATGGGGCAGAAAAGATTCTTCCCTTTGTTAATTACTTCTACTTTTTCATTTCTTTATTTTTGTGAGGGCGGAGATTAATCTCCTTCTCTCATCACAACCTTCCCTTTTTACCGCCTCATCTGATGAATTATGTTCAGATTGGCGTGTTATATACCTTGTATTAAATTAATTAATCTATGGTTCTTTTTAATGTTTTTTTCAACAATCCAATTTATATCACAAGTTATCAACTTTTTGTATCTTTAAAAATATATCACCTAAGTTTGTCGATGCTTTATGTAACTATCAACACACTTGCAATATTGTGGTGTCAATATCATGTTTGCTTTGAATTAAA

mRNA sequence

CCCAAATCTTTCAACTATATAAGTCTATATATGAATCTCCTCCTCCATTTTCATTCACTTTCATTTGCGTCTTCTAAAACATCATCGCTGCGTCCCACAGCGCCGCCGCCTCTCTCTCTCTCTCTAAAAACCATGCAAATAATAAGTTCTACAATCCCGGCTCAAAATCTCTCCCAATTTCAGGTCCGCCGGTCGAGTTTCAAATACCAAATGTCGCCGTTGAACCCCTCAGCGGCGCAGATTTCTTCCTTTAAACCCGCCACGTCGACGGTTGATGGAGCTCGCCGGCATCTGGCAAATCTGGACAAGTTGTTGAAGAAAACCGATCCGCCGGTCAAGGTCGACCCGGAACCGGTTCACAAGGACCCGAATAAGGGATCGGTGATGCCGATTAAAGGAAAGGGGCTTTTAGAAGGGCTGAACCTGGCCAGAATTTGGCCGGAGGTAAAAGCGGTGGAGGAAATGTCGCCGAGAAATCTCAACAGGCTGCAGAGGCTACTGTCGAAAACGATGGAGTATTCACCGAGAAACGCCCTCGGCCAGCGGTGGCGCGAGTACCACGGTTGCAACGATTGGTCCGGTTTACTCGACCCGCTCGATGAAAATCTCCGCCGTGAAGTCGTCCGGTACGGGGAGTTCGTACAAGCCGCATACCATGCCTTCCACTCAAACCCAGCCACGTCACCCAACGAGGCCCCACTCCCTCGCCACGTGGCACTTCCGGATAGATCTTACAAAGTCACAAAGAGTCTCTACGCCACGTCATCAGTCGGGCTACCCGGTTGGGTGGACGAGGTCGCTCCGGATCTCGGCTGGATGACGCAGCGGTCCAGTTGGGTCGGATACGTCGCCGTTTGCGACGATCGCCGTGAAATCGCACGAATGGGACGACGCGATATCGTCATCGCACTTCGTGGCACCGCCACCTGTCTGGAATGGGCCGAAAATGTTAGAGCTCAACTCATCAACATCCCCGCTGACGCTGACGCTGCCGCGGAAGACGGTGGCGATCCGAAAGTGGAGTGCGGATTTCTAAGCCTGTATAAAACTGCTGGAGCACACGTGAAGAGCTTATCAGAGTCGGTGGTGGAGGAAATCCGACGATTAACGGAGATGTACAAAGGAGAAAGGTTGAGCATCACAGTGACCGGACACAGCCTAGGCGCGGCATTGGCTGTCCTGGTAGCGGACGAGATCAGCGTTTGTTCACCGGAGGTTCCGCCAGTGGCAGTGTTCTCATTCGGCGGACCGCGCGTGGGGAACAAAATCTTCGCAAATCGAATAAAATCTCGAAACGTGAAGGTATTGCGGATTGTAAACTCACAAGACGTGATAACACAGGTACCGCCAGTGACATACTCCCACGTGGGAACCGAGCTCCGAGTGGAGACAAAGATGTCGCCGTTCTTGAAACCTAACGCCGACGTAGCTTGTTGCCATGATTTGGAAGCGTATTTACATCTGGTCGACGGATTCTTGTCGTCTAAATGTCCGTTCCGACCGAATGCGAAACGGAGTTTAGTGCGACTGGTGCAAGATCAGAGGGGGAACGTGAAGAAATTGTATATGAGGAAAGTGAAGGATTTGGGCTTCAATCCAGAGCTGCAGACGGCGGGGTGTTTGCCGAGTCCCTCTTGATTTCATCACAATTTTACTAAATCTGGGGAGTTTGGTTGAACATGGTTGGATTCTAGGCATTAGATTTCATGAACATACACAATTTCAATGGGGCAGAAAAGATTCTTCCCTTTGTTAATTACTTCTACTTTTTCATTTCTTTATTTTTGTGAGGGCGGAGATTAATCTCCTTCTCTCATCACAACCTTCCCTTTTTACCGCCTCATCTGATGAATTATGTTCAGATTGGCGTGTTATATACCTTGTATTAAATTAATTAATCTATGGTTCTTTTTAATGTTTTTTTCAACAATCCAATTTATATCACAAGTTATCAACTTTTTGTATCTTTAAAAATATATCACCTAAGTTTGTCGATGCTTTATGTAACTATCAACACACTTGCAATATTGTGGTGTCAATATCATGTTTGCTTTGAATTAAA

Coding sequence (CDS)

CCCAAATCTTTCAACTATATAAGTCTATATATGAATCTCCTCCTCCATTTTCATTCACTTTCATTTGCGTCTTCTAAAACATCATCGCTGCGTCCCACAGCGCCGCCGCCTCTCTCTCTCTCTCTAAAAACCATGCAAATAATAAGTTCTACAATCCCGGCTCAAAATCTCTCCCAATTTCAGGTCCGCCGGTCGAGTTTCAAATACCAAATGTCGCCGTTGAACCCCTCAGCGGCGCAGATTTCTTCCTTTAAACCCGCCACGTCGACGGTTGATGGAGCTCGCCGGCATCTGGCAAATCTGGACAAGTTGTTGAAGAAAACCGATCCGCCGGTCAAGGTCGACCCGGAACCGGTTCACAAGGACCCGAATAAGGGATCGGTGATGCCGATTAAAGGAAAGGGGCTTTTAGAAGGGCTGAACCTGGCCAGAATTTGGCCGGAGGTAAAAGCGGTGGAGGAAATGTCGCCGAGAAATCTCAACAGGCTGCAGAGGCTACTGTCGAAAACGATGGAGTATTCACCGAGAAACGCCCTCGGCCAGCGGTGGCGCGAGTACCACGGTTGCAACGATTGGTCCGGTTTACTCGACCCGCTCGATGAAAATCTCCGCCGTGAAGTCGTCCGGTACGGGGAGTTCGTACAAGCCGCATACCATGCCTTCCACTCAAACCCAGCCACGTCACCCAACGAGGCCCCACTCCCTCGCCACGTGGCACTTCCGGATAGATCTTACAAAGTCACAAAGAGTCTCTACGCCACGTCATCAGTCGGGCTACCCGGTTGGGTGGACGAGGTCGCTCCGGATCTCGGCTGGATGACGCAGCGGTCCAGTTGGGTCGGATACGTCGCCGTTTGCGACGATCGCCGTGAAATCGCACGAATGGGACGACGCGATATCGTCATCGCACTTCGTGGCACCGCCACCTGTCTGGAATGGGCCGAAAATGTTAGAGCTCAACTCATCAACATCCCCGCTGACGCTGACGCTGCCGCGGAAGACGGTGGCGATCCGAAAGTGGAGTGCGGATTTCTAAGCCTGTATAAAACTGCTGGAGCACACGTGAAGAGCTTATCAGAGTCGGTGGTGGAGGAAATCCGACGATTAACGGAGATGTACAAAGGAGAAAGGTTGAGCATCACAGTGACCGGACACAGCCTAGGCGCGGCATTGGCTGTCCTGGTAGCGGACGAGATCAGCGTTTGTTCACCGGAGGTTCCGCCAGTGGCAGTGTTCTCATTCGGCGGACCGCGCGTGGGGAACAAAATCTTCGCAAATCGAATAAAATCTCGAAACGTGAAGGTATTGCGGATTGTAAACTCACAAGACGTGATAACACAGGTACCGCCAGTGACATACTCCCACGTGGGAACCGAGCTCCGAGTGGAGACAAAGATGTCGCCGTTCTTGAAACCTAACGCCGACGTAGCTTGTTGCCATGATTTGGAAGCGTATTTACATCTGGTCGACGGATTCTTGTCGTCTAAATGTCCGTTCCGACCGAATGCGAAACGGAGTTTAGTGCGACTGGTGCAAGATCAGAGGGGGAACGTGAAGAAATTGTATATGAGGAAAGTGAAGGATTTGGGCTTCAATCCAGAGCTGCAGACGGCGGGGTGTTTGCCGAGTCCCTCTTGA

Protein sequence

PKSFNYISLYMNLLLHFHSLSFASSKTSSLRPTAPPPLSLSLKTMQIISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKLLKKTDPPVKVDPEPVHKDPNKGSVMPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNRLQRLLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFLSLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPEVPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLYMRKVKDLGFNPELQTAGCLPSPS
Homology
BLAST of Bhi05G000437 vs. TAIR 10
Match: AT4G16820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.6e-153
Identity = 298/523 (56.98%), Postives = 353/523 (67.50%), Query Frame = 0

Query: 56  NLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKLLKKTDPPVKVD 115
           N   F  +R  F            +  S  PA  T    + HL NL+ +L+ +     +D
Sbjct: 7   NADIFHAKRRRFTCNTHTSTLIPTKPLSVSPARKT---NKEHLRNLENVLRTSSN--SID 66

Query: 116 PEPVHKDPNKGSVMPIKGKGLLEGLNLARIWPEVK-AVEEMSPRNLNRLQRLLSKTM-EY 175
                    + +        LL GLNLARIWP++K AV+EMSP+NL RLQRLLSK+  E 
Sbjct: 67  HIENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEER 126

Query: 176 SPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSPNEAP 235
           SP++ LG +WRE HG N+W+GLLDPLDENLRRE+VRYGEFVQAAYHAFHS+P  S     
Sbjct: 127 SPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGS----- 186

Query: 236 LPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDRREIA 295
            PRHVALPD S+KVTKSLYATSSV LP W+D+VAPDL WMT+++SWVGYVAVCDD REI 
Sbjct: 187 -PRHVALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDPREIR 246

Query: 296 RMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFLSLYKTAGA 355
           RMGRR+IVIALRGTAT LEW+EN R  L+++P +      D   PKVECGF SLY T   
Sbjct: 247 RMGRREIVIALRGTATLLEWSENFRPNLVSMP-EPKPDQSDPTRPKVECGFNSLYTTGDQ 306

Query: 356 HVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPEVPPVAVFS 415
           H  SL+ES+V EI RL E+Y GE LSI+VTGHSLGAA+A+L AD+I+   P  PPVAVFS
Sbjct: 307 HAPSLAESLVGEISRLVELYAGEELSISVTGHSLGAAIALLAADDIAERVPHAPPVAVFS 366

Query: 416 FGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP------------------------ 475
           FGGPRVGN+ FA+R+ S+ VKVLR+VNSQDV+T+VP                        
Sbjct: 367 FGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNGRSPGGIME 426

Query: 476 ------PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNA 535
                 P  YSHVG ELRV+ KMSP+LKPNADVACCHDLEAYLHLVDGFL+S CPFR NA
Sbjct: 427 MVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFLASNCPFRANA 486

Query: 536 KRSLVRLVQDQRGNVKKLYMRKVKDLGFNPELQTAG-CLPSPS 546
           KRSL +L+ +QR NVK LY    K L  N      G  LPSPS
Sbjct: 487 KRSLRKLLDEQRSNVKVLYTG--KSLRLNRTFHDNGDVLPSPS 515

BLAST of Bhi05G000437 vs. TAIR 10
Match: AT2G44810.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 4.1e-88
Identity = 178/364 (48.90%), Postives = 229/364 (62.91%), Query Frame = 0

Query: 185 EYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATS-------PNEAPLPRH 244
           EY G  +W GLLDPLD+NLRRE++RYG+FV++AY AF  +P++        P    L R 
Sbjct: 2   EYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPSSPTYGTCRFPRSTLLERS 61

Query: 245 VALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDRREIARMGR 304
             LP+  Y++TK+L ATS + LP W+ E AP   WM  +SSW+GYVAVC D+ EI+R+GR
Sbjct: 62  -GLPNSGYRLTKNLRATSGINLPRWI-EKAP--SWMATQSSWIGYVAVCQDKEEISRLGR 121

Query: 305 RDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGD--PKVECGFLSLYKTAGAHV 364
           RD+VI+ RGTATCLEW EN+RA L ++P     A  +G +  P VE GFLSLY T+G H 
Sbjct: 122 RDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLY-TSGVH- 181

Query: 365 KSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPEVPPVAVFSFG 424
            SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A +I       P V V SFG
Sbjct: 182 -SLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYDIKTTFKRAPMVTVISFG 241

Query: 425 GPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP-------------------------- 484
           GPRVGN+ F   ++ +  KVLRIVNS DVIT+VP                          
Sbjct: 242 GPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQDNVKMTASIMPSWIQRR 301

Query: 485 ----PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKR 510
               P  Y+ +G ELR+ ++ SP L  + +VA CH+L+ YLHLVDGF+SS CPFR  A+R
Sbjct: 302 VEETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLHLVDGFVSSTCPFRETARR 357

BLAST of Bhi05G000437 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 1.5e-66
Identity = 150/360 (41.67%), Postives = 205/360 (56.94%), Query Frame = 0

Query: 178 ALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATS--------P 237
           +L + WRE  GCN+W G LDP++ +LRRE++RYGEF QA Y +F  +P +         P
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 238 NEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDR 297
           ++  L   + L  + Y +T+ LYATS++ LP +  +      W +Q ++W+G+VAV  D 
Sbjct: 146 SDFFLNLDLHL-HKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVAVATDE 205

Query: 298 REIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDP--KVECGFLSL 357
            E++R+GRRDIVIA RGT T LEW  +++        D   +A  G DP  K+E GF  L
Sbjct: 206 EEVSRLGRRDIVIAWRGTVTYLEWIYDLK--------DILCSANFGDDPSIKIELGFHDL 265

Query: 358 YKTAGAHVK----SLSESVVEEIRRLTEMY----KGERLSITVTGHSLGAALAVLVADEI 417
           Y       K    S  E V+ E++RL E Y    +G + SITVTGHSLGA+LA++ A +I
Sbjct: 266 YTKKEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDI 325

Query: 418 SVCS-PEVP------PVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP--- 477
           +  +   VP      P+ VFSF GPRVGN  F  R     VKVLR+VN  D +  VP   
Sbjct: 326 AELNLNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIF 385

Query: 478 -----------------PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGF 493
                            P +Y+HVG EL ++ K SPFLKP  D+ C H+LEA LHLVDG+
Sbjct: 386 TNEKFQFQKYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGY 435

BLAST of Bhi05G000437 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 3.4e-66
Identity = 147/366 (40.16%), Postives = 212/366 (57.92%), Query Frame = 0

Query: 174 SPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP------AT 233
           SPR  + + WRE HG N+W  LLDPL   LRREV +YGEFV++ Y +   +P      ++
Sbjct: 109 SPREKISKMWRELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSS 168

Query: 234 SPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCD 293
             N   L   + L    YKVTK +YA S V +P W    A    W ++ S+W+G+VAV  
Sbjct: 169 RYNRNKLFEELGLTRHGYKVTKYIYAMSRVDVPQWFLSSALGETW-SKDSNWMGFVAVSG 228

Query: 294 DRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGD-PKVECGFLS 353
           D RE  R+GRRDIV+A RGT T  EW  ++R  +       D   + G    KV+ GFLS
Sbjct: 229 D-RESLRIGRRDIVVAWRGTVTPTEWFMDLRTSM----EPFDCEGKHGKTVVKVQSGFLS 288

Query: 354 LYKTAGA----HVKSLSESVVEEIRRLTEMYK--GERLSITVTGHSLGAALAVLVADEIS 413
           +Y +       + +S SE  ++E++RL   +K  GE +S+T+TGHSLG ALA++ A E +
Sbjct: 289 IYNSKSELTRYNKESASEQTMDEVKRLVNFFKDRGEEVSLTITGHSLGGALALMNAYEAA 348

Query: 414 VCSPEVP-PVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP---------- 473
              P +   ++V SFG PRVGN  F  ++ S  VKVLR+VN QD++ ++P          
Sbjct: 349 RDVPALSGNISVISFGAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLNK 408

Query: 474 --PVT------YSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRP 508
             P+T      Y HVGT+L+++   SP++K ++D+   H+LE YLH++DGF   K  FR 
Sbjct: 409 LNPITSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFRV 468

BLAST of Bhi05G000437 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 5.6e-61
Identity = 140/354 (39.55%), Postives = 196/354 (55.37%), Query Frame = 0

Query: 179 LGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSP------NEA 238
           L   WR+  G +DW+GL+DP+D  LR E++RYGE  QA Y AF  +PA+           
Sbjct: 101 LRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRL 160

Query: 239 PLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDRREI 298
                + + D  Y+V + LYATS++ LP +  +      W ++ ++W+GYVAV DD    
Sbjct: 161 EFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSR 220

Query: 299 ARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFLSLYKTAG 358
            R+GRRDI IA RGT T LEW  +++  L  +  +     +     KVE GFL LY    
Sbjct: 221 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPA--VKVESGFLDLYTDKD 280

Query: 359 AHVK----SLSESVVEEIRRLTEMYKGE---RLSITVTGHSLGAALAVLVADEISVC--- 418
              K    S  E ++ E++RL E +  +    LSITVTGHSLG ALA+L A +I+     
Sbjct: 281 TTCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLN 340

Query: 419 ---SPEVPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP---------- 478
                +V PV V ++GGPRVGN  F  R++   VKV+R+VN  DV+ + P          
Sbjct: 341 RSKKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPH 400

Query: 479 ---------PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGFLS 495
                    P  YSHVG EL ++ + SPFLKP+ DV+  H+LEA LHL+DG++S
Sbjct: 401 ALMKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYVS 451

BLAST of Bhi05G000437 vs. ExPASy Swiss-Prot
Match: O23522 (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 540.4 bits (1391), Expect = 2.3e-152
Identity = 298/523 (56.98%), Postives = 353/523 (67.50%), Query Frame = 0

Query: 56  NLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKLLKKTDPPVKVD 115
           N   F  +R  F            +  S  PA  T    + HL NL+ +L+ +     +D
Sbjct: 7   NADIFHAKRRRFTCNTHTSTLIPTKPLSVSPARKT---NKEHLRNLENVLRTSSN--SID 66

Query: 116 PEPVHKDPNKGSVMPIKGKGLLEGLNLARIWPEVK-AVEEMSPRNLNRLQRLLSKTM-EY 175
                    + +        LL GLNLARIWP++K AV+EMSP+NL RLQRLLSK+  E 
Sbjct: 67  HIENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEER 126

Query: 176 SPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSPNEAP 235
           SP++ LG +WRE HG N+W+GLLDPLDENLRRE+VRYGEFVQAAYHAFHS+P  S     
Sbjct: 127 SPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGS----- 186

Query: 236 LPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDRREIA 295
            PRHVALPD S+KVTKSLYATSSV LP W+D+VAPDL WMT+++SWVGYVAVCDD REI 
Sbjct: 187 -PRHVALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDPREIR 246

Query: 296 RMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFLSLYKTAGA 355
           RMGRR+IVIALRGTAT LEW+EN R  L+++P +      D   PKVECGF SLY T   
Sbjct: 247 RMGRREIVIALRGTATLLEWSENFRPNLVSMP-EPKPDQSDPTRPKVECGFNSLYTTGDQ 306

Query: 356 HVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPEVPPVAVFS 415
           H  SL+ES+V EI RL E+Y GE LSI+VTGHSLGAA+A+L AD+I+   P  PPVAVFS
Sbjct: 307 HAPSLAESLVGEISRLVELYAGEELSISVTGHSLGAAIALLAADDIAERVPHAPPVAVFS 366

Query: 416 FGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP------------------------ 475
           FGGPRVGN+ FA+R+ S+ VKVLR+VNSQDV+T+VP                        
Sbjct: 367 FGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNGRSPGGIME 426

Query: 476 ------PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNA 535
                 P  YSHVG ELRV+ KMSP+LKPNADVACCHDLEAYLHLVDGFL+S CPFR NA
Sbjct: 427 MVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFLASNCPFRANA 486

Query: 536 KRSLVRLVQDQRGNVKKLYMRKVKDLGFNPELQTAG-CLPSPS 546
           KRSL +L+ +QR NVK LY    K L  N      G  LPSPS
Sbjct: 487 KRSLRKLLDEQRSNVKVLYTG--KSLRLNRTFHDNGDVLPSPS 515

BLAST of Bhi05G000437 vs. ExPASy Swiss-Prot
Match: Q948R1 (Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 3.3e-90
Identity = 185/382 (48.43%), Postives = 239/382 (62.57%), Query Frame = 0

Query: 172 EYSPRNA-----LGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPA 231
           E+SP  +     LG+RW EY G  +W GLLDPLD+NLRRE++RYG+FV++AY AF  +P+
Sbjct: 74  EFSPTGSSIPVKLGRRWMEYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPS 133

Query: 232 TS-------PNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSW 291
           +        P    L R   LP+  Y++TK+L ATS + LP W+ E AP   WM  +SSW
Sbjct: 134 SPTYGTCRFPRSTLLERS-GLPNSGYRLTKNLRATSGINLPRWI-EKAP--SWMATQSSW 193

Query: 292 VGYVAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGD-- 351
           +GYVAVC D+ EI+R+GRRD+VI+ RGTATCLEW EN+RA L ++P     A  +G +  
Sbjct: 194 IGYVAVCQDKEEISRLGRRDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSG 253

Query: 352 PKVECGFLSLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVAD 411
           P VE GFLSLY T+G H  SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A 
Sbjct: 254 PMVESGFLSLY-TSGVH--SLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAY 313

Query: 412 EISVCSPEVPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP-------- 471
           +I       P V V SFGGPRVGN+ F   ++ +  KVLRIVNS DVIT+VP        
Sbjct: 314 DIKTTFKRAPMVTVISFGGPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENRE 373

Query: 472 ----------------------PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLH 510
                                 P  Y+ +G ELR+ ++ SP L  + +VA CH+L+ YLH
Sbjct: 374 QDNVKMTASIMPSWIQRRVEETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLH 433

BLAST of Bhi05G000437 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.1e-65
Identity = 150/360 (41.67%), Postives = 205/360 (56.94%), Query Frame = 0

Query: 178 ALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATS--------P 237
           +L + WRE  GCN+W G LDP++ +LRRE++RYGEF QA Y +F  +P +         P
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 238 NEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDR 297
           ++  L   + L  + Y +T+ LYATS++ LP +  +      W +Q ++W+G+VAV  D 
Sbjct: 146 SDFFLNLDLHL-HKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVAVATDE 205

Query: 298 REIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDP--KVECGFLSL 357
            E++R+GRRDIVIA RGT T LEW  +++        D   +A  G DP  K+E GF  L
Sbjct: 206 EEVSRLGRRDIVIAWRGTVTYLEWIYDLK--------DILCSANFGDDPSIKIELGFHDL 265

Query: 358 YKTAGAHVK----SLSESVVEEIRRLTEMY----KGERLSITVTGHSLGAALAVLVADEI 417
           Y       K    S  E V+ E++RL E Y    +G + SITVTGHSLGA+LA++ A +I
Sbjct: 266 YTKKEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDI 325

Query: 418 SVCS-PEVP------PVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP--- 477
           +  +   VP      P+ VFSF GPRVGN  F  R     VKVLR+VN  D +  VP   
Sbjct: 326 AELNLNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIF 385

Query: 478 -----------------PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGF 493
                            P +Y+HVG EL ++ K SPFLKP  D+ C H+LEA LHLVDG+
Sbjct: 386 TNEKFQFQKYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGY 435

BLAST of Bhi05G000437 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 145/378 (38.36%), Postives = 200/378 (52.91%), Query Frame = 0

Query: 154 EMSPRNLNRLQRLLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEF 213
           E     L   + +  +  E      L   WR+  G +DW+GL+DP+D  LR E++RYGE 
Sbjct: 60  EQESYGLTTAEDIRRRDGEAKESKRLRDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEM 119

Query: 214 VQAAYHAFHSNP------ATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVA 273
            QA Y AF  +P      +       L   + + D  Y+V + LYATS++ LP +  +  
Sbjct: 120 AQACYDAFDFDPFSRYCGSCRFTRRHLFDSLGIIDSGYEVARYLYATSNINLPNFFSKSR 179

Query: 274 PDLGWMTQRSSWVGYVAVCDDRREI-ARMGRRDIVIALRGTATCLEWAENVRAQLINIPA 333
               W ++ ++W+GYVAV DD      R+GRRDI IA RGT T LEW  +++  L  +  
Sbjct: 180 WSKVW-SKNANWMGYVAVSDDNEATRCRLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSG 239

Query: 334 DADAAAEDGGDPKVECGFLSLY--KTAGAHVKSLS--ESVVEEIRRLTEMY---KGERLS 393
           +     +     K E GFL LY  K    +    S  E V+ E++RL E Y   +GE LS
Sbjct: 240 NGFRCPDPA--VKAESGFLDLYTDKDTSCNFSKFSAREQVLTEVKRLVERYGDEEGEELS 299

Query: 394 ITVTGHSLGAALAVLVADEIS------VCSPEVPPVAVFSFGGPRVGNKIFANRIKSRNV 453
           ITVTGHSLG ALAVL A +++          +V PV  F++GGPRVGN  F  RI+   V
Sbjct: 300 ITVTGHSLGGALAVLSAYDVAEMGVNRTRKGKVIPVTAFTYGGPRVGNIRFKERIEKLGV 359

Query: 454 KVLRIVNSQDVITQVP-------------------PVTYSHVGTELRVETKMSPFLKPNA 493
           KVLR+VN  DV+ + P                   P  YSHVG  L ++ + SPFLKP  
Sbjct: 360 KVLRVVNEHDVVAKSPGLFLNERAPQALMKLAGGLPWCYSHVGEMLPLDHQKSPFLKPTV 419

BLAST of Bhi05G000437 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 139/352 (39.49%), Postives = 194/352 (55.11%), Query Frame = 0

Query: 179 LGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSP------NEA 238
           L   WR+  G +DW+GL+DP+D  LR E++RYGE  QA Y AF  +PA+           
Sbjct: 101 LRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRL 160

Query: 239 PLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCDDRREI 298
                + + D  Y+V + LYATS++ LP +  +      W ++ ++W+GYVAV DD    
Sbjct: 161 EFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSR 220

Query: 299 ARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFLSLYKTAG 358
            R+GRRDI IA RGT T LEW  +++  L  +  +     +     KVE GFL LY    
Sbjct: 221 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPA--VKVESGFLDLYTDKD 280

Query: 359 AHVK----SLSESVVEEIRRLTEMYKGE---RLSITVTGHSLGAALAVLVADEISVC--- 418
              K    S  E ++ E++RL E +  +    LSITVTGHSLG ALA+L A +I+     
Sbjct: 281 TTCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLN 340

Query: 419 ---SPEVPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP---------- 478
                +V PV V ++GGPRVGN  F  R++   VKV+R+VN  DV+ + P          
Sbjct: 341 RSKKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPH 400

Query: 479 ---------PVTYSHVGTELRVETKMSPFLKPNADVACCHDLEAYLHLVDGF 493
                    P  YSHVG EL ++ + SPFLKP+ DV+  H+LEA LHL+DG+
Sbjct: 401 ALMKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGY 449

BLAST of Bhi05G000437 vs. ExPASy TrEMBL
Match: A0A0A0LEL7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914060 PE=4 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.6e-265
Identity = 453/503 (90.06%), Postives = 476/503 (94.63%), Query Frame = 0

Query: 45  MQIISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKL 104
           MQ+I+STIP QNLSQF +RRSSFKYQ+SPLNPSA +I+SF+ A   V   RRHLANLDKL
Sbjct: 1   MQVINSTIPPQNLSQFHLRRSSFKYQISPLNPSAPKITSFRSAAVAVHVTRRHLANLDKL 60

Query: 105 LKKTDPPVKVDPEPVHKDPNKGSVMPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNRLQ 164
           L KTDPP+KVDP+PVHKDPNK S MPIKGKGLLE LNLAR+WPEVKA E+MSPRNLNRLQ
Sbjct: 61  LNKTDPPLKVDPQPVHKDPNKVSPMPIKGKGLLESLNLARLWPEVKAAEDMSPRNLNRLQ 120

Query: 165 RLLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 224
           RLLSKT+EYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN
Sbjct: 121 RLLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 180

Query: 225 PATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVA 284
           P TSPNE PLPRHVALPDRSYKVTKSLYATSSVGLPGW+DEVAPDLGWMTQRSSWVGYVA
Sbjct: 181 PTTSPNEPPLPRHVALPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVA 240

Query: 285 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGF 344
           VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQL N+PAD D   +DGGDPKVECGF
Sbjct: 241 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNVPADVD--TKDGGDPKVECGF 300

Query: 345 LSLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSP 404
           LSLYKTAGAHVKSLSESVVEEIRRLTE+YKGE LSITVTGHSLGAALA+LVADEISVCS 
Sbjct: 301 LSLYKTAGAHVKSLSESVVEEIRRLTELYKGETLSITVTGHSLGAALAILVADEISVCSA 360

Query: 405 EVPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRV 464
           EVPPVAVFSFGGPRVGNKIFA+RIKSRNVKVLRIVNSQD+ITQVP  P+TYSHVGTELRV
Sbjct: 361 EVPPVAVFSFGGPRVGNKIFADRIKSRNVKVLRIVNSQDLITQVPPNPMTYSHVGTELRV 420

Query: 465 ETKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLY 524
           ETKMSPFLKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSLVRLVQDQRGN+KKLY
Sbjct: 421 ETKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLY 480

Query: 525 MRKVKDLGFNPELQTAGCLPSPS 546
           MRKVKDLGFNPELQT GCLPSPS
Sbjct: 481 MRKVKDLGFNPELQTVGCLPSPS 501

BLAST of Bhi05G000437 vs. ExPASy TrEMBL
Match: A0A5D3E612 (Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003630 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.7e-263
Identity = 452/502 (90.04%), Postives = 475/502 (94.62%), Query Frame = 0

Query: 47  IISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKLLK 106
           +I+STIP QNLS F +RRSSFKYQ+SPLNPSA +ISSF+ A   V   RRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 107 KTDPPVKVDPEPVHKDPNKGSV-MPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNRLQR 166
           KTDPP+KVDP+PVHKDPNKGS+ MPIKGKGLLEGLNL+R+WPEVKA EEMSPRNLNRLQR
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQR 123

Query: 167 LLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 226
           LLSKT+EYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP
Sbjct: 124 LLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 183

Query: 227 ATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAV 286
            TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLPGW+DEVAPDLGWMTQRSSWVGYVAV
Sbjct: 184 TTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAV 243

Query: 287 CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFL 346
           CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLI+IPAD D   +D GDPKVECGFL
Sbjct: 244 CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVD--TKDCGDPKVECGFL 303

Query: 347 SLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPE 406
           SLYKTAGAHV+SLSESVVEEIRRLTEMYKGE LSITVTGHSLGAALA+LVADEISVCS E
Sbjct: 304 SLYKTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAE 363

Query: 407 VPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRVE 466
           VPPVAVFSFGGPRVGNK+FA+RIKSRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRVE
Sbjct: 364 VPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVE 423

Query: 467 TKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLYM 526
           TKMSPFLKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSLVRLVQDQRGN+KKLYM
Sbjct: 424 TKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYM 483

Query: 527 RKVKDLGFNPELQTAGCLPSPS 546
           RKVKDLGFNPELQT GCLPSPS
Sbjct: 484 RKVKDLGFNPELQTVGCLPSPS 503

BLAST of Bhi05G000437 vs. ExPASy TrEMBL
Match: A0A1S3CPU9 (phospholipase A1-Ibeta2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503457 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.7e-263
Identity = 452/502 (90.04%), Postives = 475/502 (94.62%), Query Frame = 0

Query: 47  IISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKLLK 106
           +I+STIP QNLS F +RRSSFKYQ+SPLNPSA +ISSF+ A   V   RRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 107 KTDPPVKVDPEPVHKDPNKGSV-MPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNRLQR 166
           KTDPP+KVDP+PVHKDPNKGS+ MPIKGKGLLEGLNL+R+WPEVKA EEMSPRNLNRLQR
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQR 123

Query: 167 LLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 226
           LLSKT+EYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP
Sbjct: 124 LLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 183

Query: 227 ATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAV 286
            TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLPGW+DEVAPDLGWMTQRSSWVGYVAV
Sbjct: 184 TTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAV 243

Query: 287 CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFL 346
           CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLI+IPAD D   +D GDPKVECGFL
Sbjct: 244 CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVD--TKDCGDPKVECGFL 303

Query: 347 SLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPE 406
           SLYKTAGAHV+SLSESVVEEIRRLTEMYKGE LSITVTGHSLGAALA+LVADEISVCS E
Sbjct: 304 SLYKTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAE 363

Query: 407 VPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRVE 466
           VPPVAVFSFGGPRVGNK+FA+RIKSRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRVE
Sbjct: 364 VPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVE 423

Query: 467 TKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLYM 526
           TKMSPFLKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSLVRLVQDQRGN+KKLYM
Sbjct: 424 TKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYM 483

Query: 527 RKVKDLGFNPELQTAGCLPSPS 546
           RKVKDLGFNPELQT GCLPSPS
Sbjct: 484 RKVKDLGFNPELQTVGCLPSPS 503

BLAST of Bhi05G000437 vs. ExPASy TrEMBL
Match: A0A5A7T4U1 (Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00160 PE=4 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.7e-262
Identity = 451/502 (89.84%), Postives = 475/502 (94.62%), Query Frame = 0

Query: 47  IISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKLLK 106
           +I+STIP QNLS F +RRSSFKYQ+SPLNPSA +ISSF+ A   V   RRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 107 KTDPPVKVDPEPVHKDPNKGSV-MPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNRLQR 166
           KTDPP+KVDP+PVHKDPNKGS+ MPIKGKGLLEGLNL+R+WPEVKA EEMSPRNLNRLQR
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQR 123

Query: 167 LLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 226
           LLSKT+EYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP
Sbjct: 124 LLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 183

Query: 227 ATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAV 286
            TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLPGW+DEVAPDLGWMTQRSSWVGYVAV
Sbjct: 184 TTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAV 243

Query: 287 CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFL 346
           CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLI+IPAD D   +D GDPKVECGFL
Sbjct: 244 CDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVD--TKDCGDPKVECGFL 303

Query: 347 SLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPE 406
           SLYKTAGAHV+SLSESVVEEIR+LTEMYKGE LSITVTGHSLGAALA+LVADEISVCS E
Sbjct: 304 SLYKTAGAHVRSLSESVVEEIRQLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAE 363

Query: 407 VPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRVE 466
           VPPVAVFSFGGPRVGNK+FA+RIKSRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRVE
Sbjct: 364 VPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVE 423

Query: 467 TKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLYM 526
           TKMSPFLKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSLVRLVQDQRGN+KKLYM
Sbjct: 424 TKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYM 483

Query: 527 RKVKDLGFNPELQTAGCLPSPS 546
           RKVKDLGFNPELQT GCLPSPS
Sbjct: 484 RKVKDLGFNPELQTVGCLPSPS 503

BLAST of Bhi05G000437 vs. ExPASy TrEMBL
Match: A0A6J1EV72 (phospholipase A1-Ibeta2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111438059 PE=4 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 9.1e-261
Identity = 448/501 (89.42%), Postives = 472/501 (94.21%), Query Frame = 0

Query: 45  MQIISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKL 104
           MQII+STIPAQNLS+FQVRRSSFKYQMSPLNPSAA+IS F+PAT+ VDG RRHLANLDKL
Sbjct: 1   MQIINSTIPAQNLSRFQVRRSSFKYQMSPLNPSAAKISYFRPATAAVDGTRRHLANLDKL 60

Query: 105 LKKTDPPVKVDPEPVHKDPNKGSVMPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNRLQ 164
           LKKTD P KV+PEPV KDPNKGSVMPIKGKGLLEGLNLARIWPE++A E+MSPRNLNRLQ
Sbjct: 61  LKKTDTPAKVEPEPVFKDPNKGSVMPIKGKGLLEGLNLARIWPEMRAAEDMSPRNLNRLQ 120

Query: 165 RLLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 224
           RLLSKT+EYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQ+AYH+FHSN
Sbjct: 121 RLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQSAYHSFHSN 180

Query: 225 PATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVA 284
           PA S NEAPLPRHVALPD+SYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSW+GYVA
Sbjct: 181 PAMSLNEAPLPRHVALPDKSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWMGYVA 240

Query: 285 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGF 344
           VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQL NIP D +++     DPKVECGF
Sbjct: 241 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNIPTDTNSS-----DPKVECGF 300

Query: 345 LSLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSP 404
           LSLYKTAGAHVKSLSESVVEEIRRL E+YKGE LSI+VTGHSLGAALA+LVADEISVCSP
Sbjct: 301 LSLYKTAGAHVKSLSESVVEEIRRLMEIYKGETLSISVTGHSLGAALALLVADEISVCSP 360

Query: 405 EVPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRVET 464
            VPPVAVFSFGGPRVGN  FANRI SRNVKVLRIVNSQD+ITQVPPVTYSHVGTELRVET
Sbjct: 361 TVPPVAVFSFGGPRVGNTAFANRITSRNVKVLRIVNSQDLITQVPPVTYSHVGTELRVET 420

Query: 465 KMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLYMR 524
           KMSP+LKPNADVACCHDLEAYLHLVDGFLSSKCPFR NAKRSLVRLV DQRGN+KKLYMR
Sbjct: 421 KMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRANAKRSLVRLVHDQRGNMKKLYMR 480

Query: 525 KVKDLGFNPELQTAGCLPSPS 546
           KVKDL  NPELQ  GCLPSPS
Sbjct: 481 KVKDLSLNPELQMLGCLPSPS 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G16820.11.6e-15356.98alpha/beta-Hydrolases superfamily protein [more]
AT2G44810.14.1e-8848.90alpha/beta-Hydrolases superfamily protein [more]
AT1G51440.11.5e-6641.67alpha/beta-Hydrolases superfamily protein [more]
AT1G30370.13.4e-6640.16alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.15.6e-6139.55alpha/beta-Hydrolases superfamily protein [more]
Match NameE-valueIdentityDescription
O235222.3e-15256.98Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16... [more]
Q948R13.3e-9048.43Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 P... [more]
Q9C8J62.1e-6541.67Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2... [more]
Q941F13.0e-5938.36Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4... [more]
Q3EBR63.0e-5939.49Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3... [more]
Match NameE-valueIdentityDescription
A0A0A0LEL73.6e-26590.06Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914060 PE... [more]
A0A5D3E6125.7e-26390.04Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CPU95.7e-26390.04phospholipase A1-Ibeta2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503457 P... [more]
A0A5A7T4U11.7e-26289.84Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1EV729.1e-26189.42phospholipase A1-Ibeta2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 182..528
e-value: 4.1E-102
score: 344.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 204..492
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 301..451
e-value: 2.0E-34
score: 118.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..130
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..122
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 450..532
coord: 45..450
NoneNo IPR availablePANTHERPTHR31403:SF37GLYCEROLIPASE A1coord: 450..532
coord: 45..450
NoneNo IPR availableCDDcd00519Lipase_3coord: 203..490
e-value: 3.7196E-44
score: 154.557

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000437Bhi05M000437mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004620 phospholipase activity