Bhi05G000180 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000180
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Subtilisin inhibitor
Location	chr5: 5016739 .. 5018945 (+)
RNA-Seq Expression	Bhi05G000180
Synteny	Bhi05G000180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATATTCTCTTAACAATAAGTTAATCTAAAATCGCCTATATATAATCATTTCATGAACTTAAACAAGTATGCTTACAAACATAGAGGTGCGAATTCGCAACAAAAACAATGACAGGTATTCTCCATAAATCCTAATGTATGTGTTGTTGTTGTTGGTTTTTTTTTTTTTCTGAAAAAAAAACCTTATTTACACTAAATATGTATTTTAGTTCTTAATTTTTTTTAATAATTTTTTAAGATTCAATTTTCATATATTGATTTTTTAAAATTAAGTTCTTGACATCATATGTTATATATATATATATAAACATTATGAGGTTGCAGTCAATTATTTTGTGCAACTCTGATTTTAAACTCAACCATTTATTGTTATTAATTTAATAAGTGTTGATATATTTTGATATAATTAGTTTGTTAGTTAAATTATAAATTTAGTCGTTAAATTTAAGTGTACTCTTATGAATTGAATTTTTAGTTTTGGATTGAACAGGACTTTGATTTATTCAAACTTAAAAAAAAAAAAAATCATAAAGATGTACTAGATCCATTCATTTTAATTTTTTAATATGTCAGATATTTATTAGACACTAAGTTGAAAGTTAAGAAATCTATTAGATATTTTTTTAAATTTAATACTTATTGTACATAAAATTGAAAGATTATGGATTTATTAAACACTTTATATAAAGTTTACCAATGAAAGAGATAAAGATTTCACGATCCCATTTACTAACCATTTGATTTCTTATTTTTTAAAATTGTGTTTGTTTTGTCACTATCCTTTACCATAGTTTTTATCTTTACTAAATTTGAGATCACATTAAAATTCGTAGCCAAATTTCGAAAATAAACCTAAGTTCCTACCTAATTTTCCAAACATGCATTTTATGAACTAAAAATTCACGAATTAACTCATGATGATTTTTCTAATATACTGGTCTGTGAATAGAATTTTAAATAATTATTCTCTAATGAGTCAACTTTTCTTAGCAAGTACTCTTAAATCAACCACAACCAGTCTCAACAAAAAAGAAGAATAGATAAGAGTTTTAAAATAAAAGTACTCTCGTACCTCTTAATATAAGGCTCTTATCCCAGCAAGATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCATCTATCGGTTCAGAAAAATTCATTATATCTAATAAAAAAAGAGATAAATAAATCTAAATATTTCTCATAACGGACCGTTAACTGAGTATTTGGTTCTAGTCCTTACAAATTACAAATTTGATAAAAGTCTTGTTATGAAATCGACAATTAAATAGCACAAAAAAGAACGTAGAGATAGAGAAGATCGACACACAGATATACGTAGTTCACTAACAGTGTGTTAGCTACGTCCACGGACAGAGGAAGAACAATATTATTTGAGAGAATGTTTTTAGAAATTACATCAAAACGACACTTCTAGGGTTAGAGAGTTTATATAGCGCACTACTCTAAACCCTAAGGTTATAATCGTAAATAACTTCAAATAAATTCAGCAAACAGATTCAAGGCATTTCAACAAATCTCATACATGGTCTTTTTAGTTTATAACATCCTCATAAACAATCCCTATTTTAGGAATTTTTTTATGATTTTGTTCGTTGATTTTTTATTTTTTTTTGTAAACTATAGGGACCGAATTCCTTAGTGTATACATATATATATATATATANATAAGTTTAAAAAATGATTGGTATTGTTTTTGAATGGAATGATTTTGTTTAGGAAAGTCATCTTGGCCAGAGCTTGTAGGGGTACGTGGAGATGAAGCAGTGAAGGCAATAGAGAAAGAAAGGCCAGACGTGAAAGCCATTATAATACCACCATGCCAACCGGTTATAACTTTAGACTATGTGTCCAATCGAGTTCGAGTCGTCGTCGGTGGTCATGGGAAGGTGTCTCACGTACCTGTCGTCGGCTAATCAACTCTTCCCTCACTTTGTAATGTCACACCTTAAATTGCACTACGACAAAATAAAATTAAGGTGTGTATTTTCCACACCCAACTTAATCATAATCCTTCCTTTTTTTTTTTTGAGTACATAAACACATGGATTTCTTTCTCAAAATTAGTTGACAATCTACGAGAGAAGTAATGATATATTCCATGTTTTCTTAGTTCTTCATTTATGAGGTTTTTTTCATCTTTCTAATGTGAGATCCTCAACATGTTTGCTTGTT

mRNA sequence

ATATATTCTCTTAACAATAAGTTAATCTAAAATCGCCTATATATAATCATTTCATGAACTTAAACAAGTATGCTTACAAACATAGAGGTGCGAATTCGCAACAAAAACAATGACAGGAAAGTCATCTTGGCCAGAGCTTGTAGGGGTACGTGGAGATGAAGCAGTGAAGGCAATAGAGAAAGAAAGGCCAGACGTGAAAGCCATTATAATACCACCATGCCAACCGGTTATAACTTTAGACTATGTGTCCAATCGAGTTCGAGTCGTCGTCGGTGGTCATGGGAAGGTGTCTCACGTACCTGTCGTCGGCTAATCAACTCTTCCCTCACTTTGTAATGTCACACCTTAAATTGCACTACGACAAAATAAAATTAAGGTGTGTATTTTCCACACCCAACTTAATCATAATCCTTCCTTTTTTTTTTTTGAGTACATAAACACATGGATTTCTTTCTCAAAATTAGTTGACAATCTACGAGAGAAGTAATGATATATTCCATGTTTTCTTAGTTCTTCATTTATGAGGTTTTTTTCATCTTTCTAATGTGAGATCCTCAACATGTTTGCTTGTT

Coding sequence (CDS)

ATGACAGGAAAGTCATCTTGGCCAGAGCTTGTAGGGGTACGTGGAGATGAAGCAGTGAAGGCAATAGAGAAAGAAAGGCCAGACGTGAAAGCCATTATAATACCACCATGCCAACCGGTTATAACTTTAGACTATGTGTCCAATCGAGTTCGAGTCGTCGTCGGTGGTCATGGGAAGGTGTCTCACGTACCTGTCGTCGGCTAA

Protein sequence

MTGKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSHVPVVG

Homology

[Close]

BLAST of Bhi05G000180 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.7 bits (130), Expect = 3.4e-08
Identity = 28/65 (43.08%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 3  GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSH 62
          GKSSWPEL+G +G++A + IE+E P +KA+II     V+   ++ +RV V V   G V  
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILD-GTVVPEIFICSRVYVWVNDCGIVVQ 95

Query: 63 VPVVG 68
          +P++G
Sbjct: 96 IPIIG 99

[Close]

BLAST of Bhi05G000180 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 47.8 bits (112), Expect = 4.1e-06
Identity = 26/64 (40.62%), Postives = 39/64 (60.94%), Query Frame = 0


Query: 4  KSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSHV 63
          K  WPELVG  G  A   IE+E P+V AI++   +  I  ++  NRV V +G +G+V+  
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIE-NFCCNRVFVYLGSNGQVADA 82

Query: 64 PVVG 68
          P++G
Sbjct: 83 PMIG 85

[Close]

BLAST of Bhi05G000180 vs. TAIR 10
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 44.3 bits (103), Expect = 4.5e-05
Identity = 26/62 (41.94%), Postives = 35/62 (56.45%), Query Frame = 0


Query: 4  KSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSHV 63
          KSSWPELVG RG+E  + I++E   V A II     V+    + +RV V V   G V+  
Sbjct: 14 KSSWPELVGRRGEEVKEIIDRENTKVTAKIISE-NAVVLAVVICDRVYVRVNDQGIVTRT 73

Query: 64 PV 66
          P+
Sbjct: 74 PI 74

[Close]

BLAST of Bhi05G000180 vs. TAIR 10
Match: AT3G50020.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 41.6 bits (96), Expect = 2.9e-04
Identity = 29/68 (42.65%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 4  KSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNR--VRVVVGG--HGK 63
          K  WPEL GV+G EA + IE + P V  +IIP    V+ ++   NR  VRV V    +G 
Sbjct: 30 KVDWPELNGVKGLEAKRIIEHDNPKVVVVIIPDDVAVLAVN-CCNRVIVRVPVNNCPNGP 89

Query: 64 VSHVPVVG 68
          VS+ P +G
Sbjct: 90 VSNFPHIG 96

[Close]

BLAST of Bhi05G000180 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 7.3e-08
Identity = 32/65 (49.23%), Postives = 40/65 (61.54%), Query Frame = 0


Query: 3  GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSH 62
          GKSSWP LVGV G  A   IE++ P+VKA+I+    PV T D+  NRVR+ V   G V  
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPV-TKDFRCNRVRIWVNKRGLVVS 64

Query: 63 VPVVG 68
           P +G
Sbjct: 65 PPRIG 68

[Close]

BLAST of Bhi05G000180 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.1e-06
Identity = 30/65 (46.15%), Postives = 39/65 (60.00%), Query Frame = 0


Query: 3  GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSH 62
          GK SWP+LVG  G  A   IE+E P V+A+I+    PV T D+  +RVRV V   G V+ 
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPV-TADFRCDRVRVWVTERGIVAR 64

Query: 63 VPVVG 68
           P +G
Sbjct: 65 PPAIG 68

[Close]

BLAST of Bhi05G000180 vs. ExPASy Swiss-Prot
Match: P86971 (Trypsin inhibitor OS=Fagopyrum tataricum OX=62330 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.1e-06
Identity = 29/69 (42.03%), Postives = 39/69 (56.52%), Query Frame = 0


Query: 3  GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVI---TLDYVSNRVRVVVGG-HG 62
          GK SWPELVG +G  A   I+KE   V A++ PP   +    T D+  +RVRV++    G
Sbjct: 18 GKQSWPELVGTKGKTAAATIDKENTHVTAVLCPPLTTLAACRTFDFRCDRVRVLINRIGG 77

Query: 63 KVSHVPVVG 68
           V+  P VG
Sbjct: 78 VVTKTPTVG 86

[Close]

BLAST of Bhi05G000180 vs. ExPASy Swiss-Prot
Match: P81712 (Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 6.8e-06
Identity = 29/66 (43.94%), Postives = 40/66 (60.61%), Query Frame = 0


Query: 2  TGKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVS 61
          T K+SWPELVGV  +EA K I++E   V+  ++PP    +T DY   RVR+ V    KV+
Sbjct: 2  TRKTSWPELVGVTAEEAEK-IKEEMSGVEIQVVPP-GSFVTADYKPQRVRLYVDESNKVT 61

Query: 62 HVPVVG 68
            P +G
Sbjct: 62 RTPGIG 65

[Close]

BLAST of Bhi05G000180 vs. ExPASy Swiss-Prot
Match: P16064 (Subtilisin inhibitor 1 OS=Phaseolus angularis OX=3914 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.2e-05
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 4  KSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSHV 63
          K+SWPELVGV  ++A   I++E  DV+ I + P    +T DY   RVR+ V    KV+  
Sbjct: 30 KTSWPELVGVTAEQAETKIKEEMVDVQ-IQVSPHDSFVTADYNPKRVRLYVDESNKVTRT 89

Query: 64 PVVG 68
          P +G
Sbjct: 90 PSIG 92

[Close]

BLAST of Bhi05G000180 vs. ExPASy TrEMBL
Match: A0A4D8ZAU2 (Uncharacterized protein OS=Salvia splendens OX=180675 GN=Saspl_043672 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.3e-11
Identity = 39/65 (60.00%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 3  GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSH 62
          GK SWPELVGV G+EAVK IEKE   V AII+PP Q  I LDY  +RV V V   G V+ 
Sbjct: 6  GKDSWPELVGVSGEEAVKVIEKENSFVNAIIVPPTQSFIPLDYRCDRVWVFVDDDGIVTR 65

Query: 63 VPVVG 68
          VP++G
Sbjct: 66 VPLIG 70

[Close]

BLAST of Bhi05G000180 vs. ExPASy TrEMBL
Match: A0A4D8Y452 (Uncharacterized protein OS=Salvia splendens OX=180675 GN=Saspl_053977 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 6.2e-10
Identity = 38/65 (58.46%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 3  GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSH 62
          GK SWPELVGV G+EAVK IEKE   V AII+PP Q  I  DY  +RV V V   G V+ 
Sbjct: 6  GKDSWPELVGVSGEEAVKVIEKENSFVNAIIVPPTQSFIPGDYRCDRVWVFVDDDGIVTR 65

Query: 63 VPVVG 68
          VP++G
Sbjct: 66 VPLIG 70

[Close]

BLAST of Bhi05G000180 vs. ExPASy TrEMBL
Match: A0A251VLD2 (Uncharacterized protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr01g0007291 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.0e-09
Identity = 36/65 (55.38%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 3   GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSH 62
           GKSSWPELVG +G+ A   IEKE P V AIII    P   + +VSNRVRVVV   G V+ 
Sbjct: 65  GKSSWPELVGEKGEVAKAIIEKENPLVNAIIISGNSPTTDMMFVSNRVRVVVNAEGLVTK 124

Query: 63  VPVVG 68
            P++G
Sbjct: 125 TPIIG 129

[Close]

BLAST of Bhi05G000180 vs. ExPASy TrEMBL
Match: W9R3U3 (Glu S.griseus protease inhibitor OS=Morus notabilis OX=981085 GN=L484_017423 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.2e-08
Identity = 37/66 (56.06%), Postives = 45/66 (68.18%), Query Frame = 0


Query: 2  TGKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVS 61
          +GK+SWPELVGV+G+ A K IEKE P V AII+     V+T DY  +RVRV V  HG V 
Sbjct: 6  SGKNSWPELVGVQGEVAEKIIEKENPAVDAIIVTE-GSVVTADYRCDRVRVWVNNHGIVY 65

Query: 62 HVPVVG 68
           VP +G
Sbjct: 66 KVPTIG 70

[Close]

BLAST of Bhi05G000180 vs. ExPASy TrEMBL
Match: A0A5J5AD86 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035405 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.2e-08
Identity = 38/65 (58.46%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 3  GKSSWPELVGVRGDEAVKAIEKERPDVKAIIIPPCQPVITLDYVSNRVRVVVGGHGKVSH 62
          GKSSWPELVG +G+ AVK IEKE   V A I+    P ITLD+  NRVRV+V   G V  
Sbjct: 8  GKSSWPELVGTKGETAVKTIEKENHKVNAQIVLDGSP-ITLDFRCNRVRVIVDKKGIVLQ 67

Query: 63 VPVVG 68
          VPV+G
Sbjct: 68 VPVIG 71

The following BLAST results are available for this feature:

BLAST of Bhi05G000180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	3.4e-08	43.08	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	4.1e-06	40.63	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43570.1	4.5e-05	41.94	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G50020.1	2.9e-04	42.65	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

BLAST of Bhi05G000180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	7.3e-08	49.23	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P24076	1.1e-06	46.15	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P86971	3.1e-06	42.03	Trypsin inhibitor OS=Fagopyrum tataricum OX=62330 PE=1 SV=1	[more]
P81712	6.8e-06	43.94	Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1	[more]
P16064	1.2e-05	42.19	Subtilisin inhibitor 1 OS=Phaseolus angularis OX=3914 PE=1 SV=1	[more]

BLAST of Bhi05G000180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A4D8ZAU2	7.3e-11	60.00	Uncharacterized protein OS=Salvia splendens OX=180675 GN=Saspl_043672 PE=3 SV=1	[more]
A0A4D8Y452	6.2e-10	58.46	Uncharacterized protein OS=Salvia splendens OX=180675 GN=Saspl_053977 PE=3 SV=1	[more]
A0A251VLD2	4.0e-09	55.38	Uncharacterized protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr01g0007291 PE...	[more]
W9R3U3	1.2e-08	56.06	Glu S.griseus protease inhibitor OS=Morus notabilis OX=981085 GN=L484_017423 PE=...	[more]
A0A5J5AD86	1.2e-08	58.46	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035405 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 4..67 e-value: 1.2E-21 score: 77.1
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 2..67
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 7..18
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..67 e-value: 1.3E-23 score: 84.9
None	No IPR available	PANTHER	PTHR33091:SF69	SUBFAMILY NOT NAMED	coord: 2..67
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 3..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000180	Bhi05M000180	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity