Bhi05G000040 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000040
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNinja-family protein AFP2-like
Locationchr5: 1269971 .. 1271494 (+)
RNA-Seq ExpressionBhi05G000040
SyntenyBhi05G000040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAATGGCGTACCCGCAGGCTTCGGTTTCGCTAAAAATATCTTTGCTTCTTCTTCTTCCTTCTCCCTCCTACGTGTTTCCTTCTTAGCCCCACCGCCCCTTTCCTCTTCTCCCTTGCCGCCACGTCGCCGCTTCTCCTTAACCCACAATCCCTCCATCCATATAATCCAAACCCACAAATCAAATTCATTCCCACACTTATTCTTCAGAGATGAAATCAATGGAGCTTCATGAAGAAAACAACGATCTACACCTCGATTTAGCCCTTTCAATCGGCGGAACTTTCAGAAATTCACCGGAAAAATCAAATGGGTCTGATGAAGAAAGCGGAGTTACAGATGCTAAAAACAGAGGCGAGATCCGGCGACGACAGTGTAAGAGGAAACGAGAAGAAGATCGGAACAATCCGCCATTCAAGAAGGAAAAATACCTTGAAGGTAATTTGAAAAACAATGTAGATCTTCATAAACCAAATCCTGCGGTGCAATTTCCGTATCCTTACTCGTTGTTGCAGTACGTTCCGTTTCTCAATGGCTATGGGTATGCGTTTCCCTGTTTTGTACCGTACTTTGCTGCCGGAGAGTCGCCGGTGGGTTGCCGGAGTGGTTCGGATGTTGGGTTTTGGAATAAGAAATCTGGGTCTTGTGGATCTTCCCCTGTATGCAGTTCTTCTGTTGTTTCTGATAATCATCATAGTTCTTCCTCTCATGAAGGTACTTTTCAATTTCTTTCAAACCCTAATGAAGAAAACCTTAATCTGGGTTTTGTTTAATGGGTTTCAATTAGATGGTTCTGGTTTTATCATTATGAGAATTTGAGATGTTAATTAGTTTTCTTGTTGTTTTTGTTGTTCATCTTCTTTCAGGAGGTAGTAGCAATTCATGTAATTCATCAATATCAATGAATCATGTTAAACCAAATCCTTCCAGTGAGAAATTTGTTGAAGAATCATCCAAATTCAAGAAATCTTGTTCATCATCTTCATCAGTGGAGAATGGGAAACCTCCAAAGCCTAAAATTCAAAGCCAAAATGTGGCAAGTTTTGATCAAATGCCTTGTGTTTCGACCACAGGGTTTGGTCCTAATGGTACTAAAATCATAACTACTGGCTTCTTGTATAGATACTCAAACTTGGAGGTTAGTATTTTGTGTGTCTGCCATGGACAATCCTTTTCGCCGGCCGAGTTCGTTAAGCACGGCGGCGGCGGAGAAGTTTCTCACCCTTTGAAGCACATTACTGTTCTTCCACCTAATGGAATTTTACCTTCTTCAATCAGATGAAACCCCATAGATTTGATTTAGATCTTTACAGCTTAACATTATTCTTAGAGTTTCGAATTGCGTCACAATGTGGCTGGCGATTTTGCTCTGATAGAGGTGTGTAAATTCTTTTAATAATTATTAAGAGCCAAGGATTGCTAATAAATATAGCAACCATGTCACTAGCTTTATTTACTAGAATGGCTTAAAATGGAGAAGAAAATATGGAATGGTAAGTTGAAGATGAATACTTCTTTACTCAAA

mRNA sequence

ATTAATGGCGTACCCGCAGGCTTCGGTTTCGCTAAAAATATCTTTGCTTCTTCTTCTTCCTTCTCCCTCCTACGTGTTTCCTTCTTAGCCCCACCGCCCCTTTCCTCTTCTCCCTTGCCGCCACGTCGCCGCTTCTCCTTAACCCACAATCCCTCCATCCATATAATCCAAACCCACAAATCAAATTCATTCCCACACTTATTCTTCAGAGATGAAATCAATGGAGCTTCATGAAGAAAACAACGATCTACACCTCGATTTAGCCCTTTCAATCGGCGGAACTTTCAGAAATTCACCGGAAAAATCAAATGGGTCTGATGAAGAAAGCGGAGTTACAGATGCTAAAAACAGAGGCGAGATCCGGCGACGACAGTGTAAGAGGAAACGAGAAGAAGATCGGAACAATCCGCCATTCAAGAAGGAAAAATACCTTGAAGGTAATTTGAAAAACAATGTAGATCTTCATAAACCAAATCCTGCGGTGCAATTTCCGTATCCTTACTCGTTGTTGCAGTACGTTCCGTTTCTCAATGGCTATGGGTATGCGTTTCCCTGTTTTGTACCGTACTTTGCTGCCGGAGAGTCGCCGGTGGGTTGCCGGAGTGGTTCGGATGTTGGGTTTTGGAATAAGAAATCTGGGTCTTGTGGATCTTCCCCTGTATGCAGTTCTTCTGTTGTTTCTGATAATCATCATAGTTCTTCCTCTCATGAAGGAGGTAGTAGCAATTCATGTAATTCATCAATATCAATGAATCATGTTAAACCAAATCCTTCCAGTGAGAAATTTGTTGAAGAATCATCCAAATTCAAGAAATCTTGTTCATCATCTTCATCAGTGGAGAATGGGAAACCTCCAAAGCCTAAAATTCAAAGCCAAAATGTGGCAAGTTTTGATCAAATGCCTTGTGTTTCGACCACAGGGTTTGGTCCTAATGGTACTAAAATCATAACTACTGGCTTCTTGTATAGATACTCAAACTTGGAGGTTAGTATTTTGTGTGTCTGCCATGGACAATCCTTTTCGCCGGCCGAGTTCGTTAAGCACGGCGGCGGCGGAGAAGTTTCTCACCCTTTGAAGCACATTACTGTTCTTCCACCTAATGGAATTTTACCTTCTTCAATCAGATGAAACCCCATAGATTTGATTTAGATCTTTACAGCTTAACATTATTCTTAGAGTTTCGAATTGCGTCACAATGTGGCTGGCGATTTTGCTCTGATAGAGGTGTGTAAATTCTTTTAATAATTATTAAGAGCCAAGGATTGCTAATAAATATAGCAACCATGTCACTAGCTTTATTTACTAGAATGGCTTAAAATGGAGAAGAAAATATGGAATGGTAAGTTGAAGATGAATACTTCTTTACTCAAA

Coding sequence (CDS)

ATGAAATCAATGGAGCTTCATGAAGAAAACAACGATCTACACCTCGATTTAGCCCTTTCAATCGGCGGAACTTTCAGAAATTCACCGGAAAAATCAAATGGGTCTGATGAAGAAAGCGGAGTTACAGATGCTAAAAACAGAGGCGAGATCCGGCGACGACAGTGTAAGAGGAAACGAGAAGAAGATCGGAACAATCCGCCATTCAAGAAGGAAAAATACCTTGAAGGTAATTTGAAAAACAATGTAGATCTTCATAAACCAAATCCTGCGGTGCAATTTCCGTATCCTTACTCGTTGTTGCAGTACGTTCCGTTTCTCAATGGCTATGGGTATGCGTTTCCCTGTTTTGTACCGTACTTTGCTGCCGGAGAGTCGCCGGTGGGTTGCCGGAGTGGTTCGGATGTTGGGTTTTGGAATAAGAAATCTGGGTCTTGTGGATCTTCCCCTGTATGCAGTTCTTCTGTTGTTTCTGATAATCATCATAGTTCTTCCTCTCATGAAGGAGGTAGTAGCAATTCATGTAATTCATCAATATCAATGAATCATGTTAAACCAAATCCTTCCAGTGAGAAATTTGTTGAAGAATCATCCAAATTCAAGAAATCTTGTTCATCATCTTCATCAGTGGAGAATGGGAAACCTCCAAAGCCTAAAATTCAAAGCCAAAATGTGGCAAGTTTTGATCAAATGCCTTGTGTTTCGACCACAGGGTTTGGTCCTAATGGTACTAAAATCATAACTACTGGCTTCTTGTATAGATACTCAAACTTGGAGGTTAGTATTTTGTGTGTCTGCCATGGACAATCCTTTTCGCCGGCCGAGTTCGTTAAGCACGGCGGCGGCGGAGAAGTTTCTCACCCTTTGAAGCACATTACTGTTCTTCCACCTAATGGAATTTTACCTTCTTCAATCAGATGA

Protein sequence

MKSMELHEENNDLHLDLALSIGGTFRNSPEKSNGSDEESGVTDAKNRGEIRRRQCKRKREEDRNNPPFKKEKYLEGNLKNNVDLHKPNPAVQFPYPYSLLQYVPFLNGYGYAFPCFVPYFAAGESPVGCRSGSDVGFWNKKSGSCGSSPVCSSSVVSDNHHSSSSHEGGSSNSCNSSISMNHVKPNPSSEKFVEESSKFKKSCSSSSSVENGKPPKPKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPNGILPSSIR
Homology
BLAST of Bhi05G000040 vs. TAIR 10
Match: AT3G07250.1 (nuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-containing protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-24
Identity = 103/308 (33.44%), Postives = 142/308 (46.10%), Query Frame = 0

Query: 12  DLHLDLALSIGGTFRNSP-------------EKSNGSDEESGVTDAKNRGEIRRRQCKRK 71
           +L L+L LS+GG F+ +              EK NG + +   T+     E R+++  ++
Sbjct: 14  ELELELCLSLGGPFKKTEKSKTFGQSNAVGFEKDNGVNLDGRTTNVTRIKETRKKREAKQ 73

Query: 72  REEDRNNPPFKKEKYLEGNLKNNVDLHKPNPAVQFPYPYSLLQYVPFLNGYGYAFPCFVP 131
           ++        K+ +     + N  D+               L  +   NGYG        
Sbjct: 74  QQRSGEEGECKRIRTEYNGVSNGDDMD--------------LSSINMCNGYG-------- 133

Query: 132 YFAAGESPVGCRSGSDVGFWNKKSGSCGSSPVCSSSVVSDNHHSSSSHEGGSSNSCNSSI 191
              +G       S  DV            SP+C+SS VSD   SSS  EGGS +    S 
Sbjct: 134 ---SGRLK---ESSKDVTI---------GSPICTSSSVSD-PSSSSRQEGGSGDIGAQSG 193

Query: 192 SMNHVKPN-----PSSEKFVEESSKFKKS-----CSSSSSVENGKPPKPKIQSQNVAS-- 251
               V+         +E+ V  +   K +      +SS + E+GKPPKP   S    S  
Sbjct: 194 QTKQVRSPVNNILTGTEQTVHNTDGSKDAETQEWSNSSVTKESGKPPKPHTNSNGNGSLL 253

Query: 252 -FDQMPCVSTTGFGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVS 294
            F QMPCV++TG GP G  +   GFLYRYS  E+SI+CVCHG SFSPAEF+ H GG  VS
Sbjct: 254 PFAQMPCVTSTGNGPEGKTV--NGFLYRYSKSEISIICVCHGTSFSPAEFIVHAGGTNVS 281

BLAST of Bhi05G000040 vs. TAIR 10
Match: AT3G29575.1 (ABI five binding protein 3 )

HSP 1 Score: 97.1 bits (240), Expect = 2.7e-20
Identity = 56/97 (57.73%), Postives = 63/97 (64.95%), Query Frame = 0

Query: 201 KSCSSSSSVENGKPPK-PKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRY-SNLE 260
           KS  +S S    +P    K  SQN+   + MP VSTTG GPNG KI   GFLYRY    E
Sbjct: 135 KSVETSPSSAQSQPENLGKEASQNI--IEDMPFVSTTGDGPNGKKI--NGFLYRYRKGEE 194

Query: 261 VSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLP 296
           V I+CVCHG   SPAEFVKH GGG+V+HPLKHI V P
Sbjct: 195 VRIVCVCHGSFLSPAEFVKHAGGGDVAHPLKHIVVNP 227

BLAST of Bhi05G000040 vs. TAIR 10
Match: AT3G29575.3 (ABI five binding protein 3 )

HSP 1 Score: 97.1 bits (240), Expect = 2.7e-20
Identity = 56/97 (57.73%), Postives = 63/97 (64.95%), Query Frame = 0

Query: 201 KSCSSSSSVENGKPPK-PKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRY-SNLE 260
           KS  +S S    +P    K  SQN+   + MP VSTTG GPNG KI   GFLYRY    E
Sbjct: 135 KSVETSPSSAQSQPENLGKEASQNI--IEDMPFVSTTGDGPNGKKI--NGFLYRYRKGEE 194

Query: 261 VSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLP 296
           V I+CVCHG   SPAEFVKH GGG+V+HPLKHI V P
Sbjct: 195 VRIVCVCHGSFLSPAEFVKHAGGGDVAHPLKHIVVNP 227

BLAST of Bhi05G000040 vs. TAIR 10
Match: AT3G29575.4 (ABI five binding protein 3 )

HSP 1 Score: 97.1 bits (240), Expect = 2.7e-20
Identity = 56/97 (57.73%), Postives = 63/97 (64.95%), Query Frame = 0

Query: 201 KSCSSSSSVENGKPPK-PKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRY-SNLE 260
           KS  +S S    +P    K  SQN+   + MP VSTTG GPNG KI   GFLYRY    E
Sbjct: 135 KSVETSPSSAQSQPENLGKEASQNI--IEDMPFVSTTGDGPNGKKI--NGFLYRYRKGEE 194

Query: 261 VSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLP 296
           V I+CVCHG   SPAEFVKH GGG+V+HPLKHI V P
Sbjct: 195 VRIVCVCHGSFLSPAEFVKHAGGGDVAHPLKHIVVNP 227

BLAST of Bhi05G000040 vs. TAIR 10
Match: AT1G13740.1 (ABI five binding protein 2 )

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-14
Identity = 59/156 (37.82%), Postives = 78/156 (50.00%), Query Frame = 0

Query: 152 SSSVVSDNHHSSSSHEGGSSNSCN-------------SSISMNHVKPNPSSEKFVEESSK 211
           SSS +S+     + ++ GSSNSCN             +S S    +P+ +    V E+  
Sbjct: 197 SSSSLSE---LDNKNQQGSSNSCNDERSPKIVAGCSSNSGSQGTERPSVTRANKVNENEN 256

Query: 212 FKKSCSSSSSVENGKPPKPKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRY-SNL 271
            K+  S  S    GK                MPCV T G GPNG ++   G LY+Y    
Sbjct: 257 EKRVRSEDSVDRKGK------GMATSTGLVDMPCVFTKGDGPNGRRV--DGILYKYGKGE 316

Query: 272 EVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITV 294
           EV I+C+CHG   +PAEFVKHGGGG+V  PL+HI V
Sbjct: 317 EVRIMCICHGSFLTPAEFVKHGGGGDVDRPLRHIVV 341

BLAST of Bhi05G000040 vs. ExPASy Swiss-Prot
Match: Q94F39 (Ninja-family protein AFP3 OS=Arabidopsis thaliana OX=3702 GN=AFP3 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.8e-19
Identity = 56/97 (57.73%), Postives = 63/97 (64.95%), Query Frame = 0

Query: 201 KSCSSSSSVENGKPPK-PKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRY-SNLE 260
           KS  +S S    +P    K  SQN+   + MP VSTTG GPNG KI   GFLYRY    E
Sbjct: 135 KSVETSPSSAQSQPENLGKEASQNI--IEDMPFVSTTGDGPNGKKI--NGFLYRYRKGEE 194

Query: 261 VSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLP 296
           V I+CVCHG   SPAEFVKH GGG+V+HPLKHI V P
Sbjct: 195 VRIVCVCHGSFLSPAEFVKHAGGGDVAHPLKHIVVNP 227

BLAST of Bhi05G000040 vs. ExPASy Swiss-Prot
Match: Q10JI3 (Ninja-family protein Os03g0419100 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0419100 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.5e-15
Identity = 43/67 (64.18%), Postives = 50/67 (74.63%), Query Frame = 0

Query: 230 MPCVSTTGFGPNGTKIITTGFLYRYSNLE-VSILCVCHGQSFSPAEFVKHGGGGEVSHPL 289
           MP VS+   GPNG KI   GFLY+Y   E VSI+CVCHG+  SPAEFVKH GGG+VS+PL
Sbjct: 237 MPMVSSKVEGPNGRKI--DGFLYKYRKGEDVSIMCVCHGKFHSPAEFVKHAGGGDVSNPL 296

Query: 290 KHITVLP 296
           +HI V P
Sbjct: 297 RHIVVNP 301

BLAST of Bhi05G000040 vs. ExPASy Swiss-Prot
Match: Q6YVY6 (Ninja-family protein Os07g0602900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0602900 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.4e-15
Identity = 40/73 (54.79%), Postives = 50/73 (68.49%), Query Frame = 0

Query: 228 DQMPCVSTTGFGPNGTKIITTGFLYRY-SNLEVSILCVCHGQSFSPAEFVKHGGGGEVSH 287
           + MP VS+   GPNG KI   GFLY+Y    EV I+CVCHG   +PAEFVKH GGG+V++
Sbjct: 247 EDMPMVSSRVDGPNGRKI--DGFLYKYRKREEVRIVCVCHGNFLTPAEFVKHAGGGDVTN 306

Query: 288 PLKHITVLPPNGI 300
           PL+HI V P   +
Sbjct: 307 PLRHIVVNPSRSV 317

BLAST of Bhi05G000040 vs. ExPASy Swiss-Prot
Match: Q9LMX5 (Ninja-family protein AFP2 OS=Arabidopsis thaliana OX=3702 GN=AFP2 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.4e-13
Identity = 59/156 (37.82%), Postives = 78/156 (50.00%), Query Frame = 0

Query: 152 SSSVVSDNHHSSSSHEGGSSNSCN-------------SSISMNHVKPNPSSEKFVEESSK 211
           SSS +S+     + ++ GSSNSCN             +S S    +P+ +    V E+  
Sbjct: 197 SSSSLSE---LDNKNQQGSSNSCNDERSPKIVAGCSSNSGSQGTERPSVTRANKVNENEN 256

Query: 212 FKKSCSSSSSVENGKPPKPKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRY-SNL 271
            K+  S  S    GK                MPCV T G GPNG ++   G LY+Y    
Sbjct: 257 EKRVRSEDSVDRKGK------GMATSTGLVDMPCVFTKGDGPNGRRV--DGILYKYGKGE 316

Query: 272 EVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITV 294
           EV I+C+CHG   +PAEFVKHGGGG+V  PL+HI V
Sbjct: 317 EVRIMCICHGSFLTPAEFVKHGGGGDVDRPLRHIVV 341

BLAST of Bhi05G000040 vs. ExPASy Swiss-Prot
Match: Q9LQ98 (Ninja-family protein AFP1 OS=Arabidopsis thaliana OX=3702 GN=AFP1 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.0e-13
Identity = 56/146 (38.36%), Postives = 74/146 (50.68%), Query Frame = 0

Query: 162 SSSSHEGGSSNSCNSSISMNHVKPNPSSEKFVEESSKFKKSCSSSSSVENGKPPKPKIQS 221
           S  S  GG +   +SS+          SE   + SS   +S  S++  +     KP  + 
Sbjct: 203 SGESLGGGRAAGSSSSV----------SELETKASSDEARSLPSTTQPQQETTTKPTNRL 262

Query: 222 QNVASFD-------------QMPCVSTTGFGPNGTKIITTGFLYRY-SNLEVSILCVCHG 281
           + ++S D             +MPCV T G GPNG ++   G LYRY S  EV I+CVCHG
Sbjct: 263 RRLSSVDMNMKMEPQGKGKSEMPCVFTKGDGPNGKRV--DGILYRYGSGEEVRIMCVCHG 322

Query: 282 QSFSPAEFVKHGGGGEVSHPLKHITV 294
              SPA+FVKH GG  V HPL+HI V
Sbjct: 323 DFLSPADFVKHAGGPHVDHPLRHIVV 336

BLAST of Bhi05G000040 vs. ExPASy TrEMBL
Match: A0A5D3DQY3 (Ninja-family protein AFP2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1185G00180 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.1e-117
Identity = 237/309 (76.70%), Postives = 256/309 (82.85%), Query Frame = 0

Query: 1   MKSMELHEENNDLHLDLALSIGGTFRNSPEKSNGSDEESGVTDAKNRGEIRRRQCKRKRE 60
           MKSM LH++N    LDL LSIGG F N PEK NGSD+ESGVTD  NR +IRRR  KRKRE
Sbjct: 1   MKSMNLHQQN----LDLRLSIGGPFNNLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKRE 60

Query: 61  ED-RNNPPFKKEKYLEGNLKNNVDLHKPNPAVQFPYPYSLLQYVPFLNGYGYAFPCFVPY 120
           ED  NNPPFK  +   GN  NNVDL+KP+P +Q  YPYS LQYVPF NGYGYA+PC VPY
Sbjct: 61  EDPNNNPPFKNRR---GNFTNNVDLNKPDPPLQISYPYSSLQYVPFFNGYGYAYPCLVPY 120

Query: 121 FAAGESPVGCRSGSDVGFWNKKSGSCGSSPVCSSSVVSDN-HHSSSSHEGGSSNSCNSSI 180
           +   ESPVGCR  SD G  NKKSGSCGSSPVCSSSVVSDN HHSSSS EGG SNS NSSI
Sbjct: 121 WPPDESPVGCRIVSDDGVSNKKSGSCGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSI 180

Query: 181 SMNHVKPNPSSEKFVE-ESSKFKKSCSSSSSVENGKPPKPKIQSQ-NVASFDQMPCVSTT 240
           SM HVKP P ++KFVE ESS+ KK  SSSSS+ENGKPPKP+IQ+Q NVASF+QMPCVSTT
Sbjct: 181 SMKHVKPKPCNKKFVEDESSQVKK--SSSSSMENGKPPKPQIQTQNNVASFEQMPCVSTT 240

Query: 241 GFGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPP 300
           G GPNGTKI+TTGFLYRYSNLEVSILCVCHG+SFSPAEFVKHGGGGEVSHPLKHITVLPP
Sbjct: 241 GIGPNGTKIVTTGFLYRYSNLEVSILCVCHGRSFSPAEFVKHGGGGEVSHPLKHITVLPP 300

Query: 301 NGILPSSIR 306
           NG  PSSIR
Sbjct: 301 NGFFPSSIR 300

BLAST of Bhi05G000040 vs. ExPASy TrEMBL
Match: A0A0A0L8T7 (TDBD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G633290 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 7.4e-111
Identity = 228/303 (75.25%), Postives = 248/303 (81.85%), Query Frame = 0

Query: 7   HEENNDLHLDLALSIGGTFRNSPEKSNGSDEESGVTDAKNRGEIRRRQCKRKREED-RNN 66
           H++N    LDL LSIGG F + PEK NGSD+ESGVTD  NR +IRRR  KRKREED  NN
Sbjct: 3   HQQN----LDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNN 62

Query: 67  PPFKKEKYLEGNLKNNVDLHKPNPAVQFPYPYSLLQYVPFLNGYGYAFPCFVPYFAAGES 126
           PPFK  K   GNL NNVDL+KP+P +   YPYS  QY+PF N YGYA+PC VPY+  GES
Sbjct: 63  PPFKNRK---GNLTNNVDLNKPDPPLPISYPYSSFQYLPFFNAYGYAYPCLVPYWPPGES 122

Query: 127 PVGCRSGSDVGFWNKKSGSCGSSPVCSSSVVSDN-HHSSSSHEGGSSNSCNSSISMNHVK 186
           PV CRS SD G  NKKSGS GSSPVCSSSVVSDN HHSSSS EGG SNS NSSISM HVK
Sbjct: 123 PVRCRSVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVK 182

Query: 187 PNPSSEKFVE-ESSKFKKSCSSSSSVENGKPPKPKIQSQ-NVASFDQMPCVSTTGFGPNG 246
           P P ++KFVE +SS+ KK  S SSS+ENGKPPKP+IQ+Q N+ASF+QMPCVSTTG GPNG
Sbjct: 183 PKPCNKKFVEDQSSQVKK--SFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNG 242

Query: 247 TKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPNGILPS 306
           TKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPP GI PS
Sbjct: 243 TKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTGIFPS 296

BLAST of Bhi05G000040 vs. ExPASy TrEMBL
Match: A0A6J1HHX8 (ninja-family protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111463802 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 9.1e-101
Identity = 222/322 (68.94%), Postives = 242/322 (75.16%), Query Frame = 0

Query: 1   MKSMELHEENNDLHLDLALSIGGTFRNSPEK----SNGSDEESGVTDAKNRGEIRRRQCK 60
           MKS  + EE  DL LDL LSIGG FRN PEK    +NGSDEESGVTD K R  IRR + K
Sbjct: 1   MKSAVIREEGGDLDLDLGLSIGGAFRNLPEKLTLINNGSDEESGVTDEKIRSAIRRGEAK 60

Query: 61  RKREEDRNNPPFKKEKYLEG-----NLKNNVDLHKPNPAVQFPYPYSLLQYVPFLNGYGY 120
           RKREE+RN  P K+EK LEG     +L NNVDL       Q PYPYS LQYVPFLNGYGY
Sbjct: 61  RKREEERNR-PLKREKKLEGLGSECDLMNNVDL-------QNPYPYSSLQYVPFLNGYGY 120

Query: 121 AFPCFVPYFAAGE-----SPVGCRSGSDV-GFWNKKSGSCGSSPVCSSSVVSDNHHSSSS 180
            FPC  PY+A GE       V CRS  DV G  N+K+GS GSSPVCSSSV+SDN+HSSSS
Sbjct: 121 PFPCLTPYWAVGERTLHQPAVACRSVPDVGGSKNRKTGSSGSSPVCSSSVISDNYHSSSS 180

Query: 181 HEGGS--SNSCNSSISMNHVKPNPSSEKFVEESSKFKKSCSSSSSVENGKPPKPKIQSQN 240
           HEG S  SNSCNSS S NHVKP   SEKFVE  S   K    +SS+ NGKPPKP+IQS++
Sbjct: 181 HEGVSSRSNSCNSS-SKNHVKPKACSEKFVE--SHVSKPRIKNSSMVNGKPPKPEIQSRH 240

Query: 241 VASFDQMPCVSTTGFGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGE 300
           V SF+QMPCVSTTG  PNGTK ITTGFLYRYSN EVSILCVCHG+SFSPAEFV+HGGGGE
Sbjct: 241 VQSFEQMPCVSTTGIAPNGTKTITTGFLYRYSNSEVSILCVCHGESFSPAEFVRHGGGGE 300

Query: 301 VSHPLKHITVLPPNGILPSSIR 306
           VSHPLKHITVLP NGI+PSSIR
Sbjct: 301 VSHPLKHITVLPTNGIVPSSIR 311

BLAST of Bhi05G000040 vs. ExPASy TrEMBL
Match: A0A6J1HQF5 (ninja-family protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111466860 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.6e-100
Identity = 222/322 (68.94%), Postives = 244/322 (75.78%), Query Frame = 0

Query: 1   MKSMELHEENNDLHLDLALSIGGTFRNSPEK----SNGSDEESGVTDAKNRGEIRRRQCK 60
           MK   + EE+ DL LDL LSIGG FRN PEK    +NGSDEESGVTD K R  IRR + K
Sbjct: 1   MKLAVIREEDGDLDLDLGLSIGGGFRNLPEKLTLINNGSDEESGVTDEKIRSAIRRGEAK 60

Query: 61  RKREEDRNNPPFKKEKYLEG-----NLKNNVDLHKPNPAVQFPYPYSLLQYVPFLNGYGY 120
           RKREE+RN  P K+EK LEG     +L NNVDL       Q PYPYS LQYVPF+NGYGY
Sbjct: 61  RKREEERNR-PLKREKKLEGLGSECDLMNNVDL-------QNPYPYSSLQYVPFINGYGY 120

Query: 121 AFPCFVPYFAAGE-----SPVGCRSGSDV-GFWNKKSGSCGSSPVCSSSVVSDNHHSSSS 180
           AFPC  PY+AAGE       V CRS SDV G  N+K+GS GSSPVCSSSV+SDN+HSSSS
Sbjct: 121 AFPCLTPYWAAGERILHQPAVACRSVSDVGGSKNRKTGSSGSSPVCSSSVISDNYHSSSS 180

Query: 181 HEGGS--SNSCNSSISMNHVKPNPSSEKFVEESSKFKKSCSSSSSVENGKPPKPKIQSQN 240
           HEG S  SNSCNSS S NHVK  P SEK VE  S   K    +SS+ NGKPPKP+IQS++
Sbjct: 181 HEGVSSRSNSCNSS-SKNHVKSKPCSEKLVE--SHVSKPRIKNSSMVNGKPPKPEIQSRH 240

Query: 241 VASFDQMPCVSTTGFGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGE 300
           V SF+QMPCVSTTG  PNGTK ITTGFLYRYSNLEVSILC CHG+SFSPAEFVKHGGGG+
Sbjct: 241 VQSFEQMPCVSTTGIAPNGTKTITTGFLYRYSNLEVSILCGCHGESFSPAEFVKHGGGGQ 300

Query: 301 VSHPLKHITVLPPNGILPSSIR 306
           VSHPLKHITVLP NGI+PSSIR
Sbjct: 301 VSHPLKHITVLPTNGIVPSSIR 311

BLAST of Bhi05G000040 vs. ExPASy TrEMBL
Match: A0A6J1K6T7 (ninja-family protein AFP2-like OS=Cucurbita maxima OX=3661 GN=LOC111491188 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 2.0e-76
Identity = 208/390 (53.33%), Postives = 238/390 (61.03%), Query Frame = 0

Query: 1   MKSMELHEE-NNDLHLDLALSIGGTFRNSPEKS------------NGSDEESGVTDAKNR 60
           MKS+ L +E NND+ +DLALSIGG FR  PEKS            +G +E+SGVTDAKNR
Sbjct: 1   MKSVVLPDENNNDVDVDLALSIGGVFRTLPEKSTASGSSFTLWINDGDEEDSGVTDAKNR 60

Query: 61  GEIRRRQCKRKR-EEDRNNPPFKKEKYLEG-NLKNNVDLH--KPNPAVQFPYPYSLLQYV 120
           G+IRRR+ KRKR EED  NPP K+EK L+G   + NVD    +   A   PYPYS LQYV
Sbjct: 61  GQIRRRETKRKRDEEDERNPPLKREKKLQGVGYECNVDREECRNETAELMPYPYSSLQYV 120

Query: 121 PFLNGYGYAFPCFVPYFAAG--------ESPVGCRS------------GSDVGFWNKKSG 180
           PF+N  G+  PC VPY+AA           PV CRS            G   G  N+K+G
Sbjct: 121 PFMN--GFVLPCVVPYWAAAGHGGERTVHQPVACRSFRPFETDRRGLDGFSNGRTNRKTG 180

Query: 181 SCGSSPVCSSSVVSDNHHSSSSHEG-GSS----NSCNSSI------------------SM 240
           SCGSSPVCSSSV+SDN + SSSHEG GSS    NSCNSS                   SM
Sbjct: 181 SCGSSPVCSSSVISDN-YQSSSHEGCGSSYTRNNSCNSSSDRHQMRCPNSNSNPNTNDSM 240

Query: 241 NHVKPNPSSEKFVE------ESSKFKK---SCSSSSSV----------------ENGKPP 300
           NH KP P SE   +      ES   KK   + SS+ SV                EN + P
Sbjct: 241 NHDKPKPCSENRTQCEEKSGESHVHKKEPITASSNKSVFNSQRSPSMSIKNMKMENEELP 300

Query: 301 KPKIQSQNVASFDQMPCVSTTGFGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEF 306
           KP+I S+NV S  QMPCVSTTG GPNG  I  TGFLYRYSNLEVSILCVCHG+SFSPAEF
Sbjct: 301 KPQILSRNVPSLQQMPCVSTTGVGPNGKTI--TGFLYRYSNLEVSILCVCHGESFSPAEF 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G07250.11.1e-2433.44nuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-c... [more]
AT3G29575.12.7e-2057.73ABI five binding protein 3 [more]
AT3G29575.32.7e-2057.73ABI five binding protein 3 [more]
AT3G29575.42.7e-2057.73ABI five binding protein 3 [more]
AT1G13740.11.7e-1437.82ABI five binding protein 2 [more]
Match NameE-valueIdentityDescription
Q94F393.8e-1957.73Ninja-family protein AFP3 OS=Arabidopsis thaliana OX=3702 GN=AFP3 PE=1 SV=1[more]
Q10JI31.5e-1564.18Ninja-family protein Os03g0419100 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q6YVY67.4e-1554.79Ninja-family protein Os07g0602900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q9LMX52.4e-1337.82Ninja-family protein AFP2 OS=Arabidopsis thaliana OX=3702 GN=AFP2 PE=1 SV=1[more]
Q9LQ984.0e-1338.36Ninja-family protein AFP1 OS=Arabidopsis thaliana OX=3702 GN=AFP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DQY33.1e-11776.70Ninja-family protein AFP2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0L8T77.4e-11175.25TDBD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G633290 PE=3 S... [more]
A0A6J1HHX89.1e-10168.94ninja-family protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111463802 PE=3 S... [more]
A0A6J1HQF51.6e-10068.94ninja-family protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111466860 PE=3 SV=... [more]
A0A6J1K6T72.0e-7653.33ninja-family protein AFP2-like OS=Cucurbita maxima OX=3661 GN=LOC111491188 PE=3 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032308Jas TPL-binding domainPFAMPF16135TDBDcoord: 259..292
e-value: 1.4E-8
score: 34.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 156..191
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..221
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 156..187
NoneNo IPR availablePANTHERPTHR31413:SF15NUCLEAR TRANSPORT FACTOR 2/RNA RECOGNITION MOTIF PROTEINcoord: 185..295
coord: 5..179
IPR031307Ninja familyPANTHERPTHR31413AFP HOMOLOG 2coord: 185..295
coord: 5..179

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000040Bhi05M000040mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045892 negative regulation of transcription, DNA-templated
biological_process GO:0009737 response to abscisic acid
biological_process GO:0007165 signal transduction
cellular_component GO:0005634 nucleus