Bhi05G000035 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000035
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionGRAM domain-containing protein
Locationchr5: 1122707 .. 1126994 (+)
RNA-Seq ExpressionBhi05G000035
SyntenyBhi05G000035
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAATCACCTTGACACCAAAGACAATCCCTATGTTCATTATTCCTCTGCTTCCACATCTCCAAATGGGAAATGTTTGTTTTCTTCTGTTCTGCACTTAACGTGTTTGTAAAAATTCCTCTGTGGATATTGATACTCTTTCTTTTTTTCTTTTCTTTTTTTTTTCTTTTGCATATGTTCAGCAGATAAGATGTCGGAAGTGTTGGATCGGTGTGGGAAGAGGTTTGCAGATTGTTCTAAGATGGTAGAGGCTGCAGCAGATGGCATCTGGAATCACTGTTAGTTCTTTCCTCTATTCAAGTTTCTTTCTTTAAGGTGTTTGTTTTCACCTAGAATTCTAACAAGTTTATGGTAGGTAGGAATGCTATTAGGATGTCAGAGGGATATTAAGGTATACTAGTTATTAGTTAGGGGAGTTGGTTATGGAATTTAGTTATAAATAGTGATAAGGAGAATGGGAAGATAATCAATATTTTGGTTAATGAATTAGGGCTTGAGAGTATTTCAAAATTGGGAGGGTCCAAGTACATCGAACACTTGGTTTATCTTGTATTTCTATTATCTTTTATGTCTCTATTTATTTGGGTTCTATTAATTTAGTTCTCTCCATCTTTCTAATATGATTGGTCGAGAGCTTTCAACGAGTCAGTTCTGAATAATTGGAAGAGTTTAGGTCTGTCATGAAATGGTTTCAGGCTTGTCATGAGTTATCATTGATTCCAAATAATCACTGATGTTTATTAATTTAATAGGTACAGCTTTCAATGATTAGTTTCAAACGATTAGAACTGTTTCTTTCTGTAAACAAATGATAACTTCAAATAACAAATGGCATGCTAAAAGTTAAAAACAGAACCAACTTTTCTTGGCAAGGATTTGCTTGGTTCCTTTTGGTTCTTTCTGGAATAATGAAATTCGGACCTGGATTTATATACCTGTTCGATGTCTATCGAGCTTATTAGCGTCTAACCTGCAATGCAATATATGCTTTTCCCAACAGTGAAACTTAGTTCTAGTGTCACTGATGCGGCCATGGCCAGGCTTCATCAAGGAACGAAGTTAGTTACAGAAGGTGGCTATAAAAAAGTATTCCAACAGACGTTTGGTTTCGTCGACGGAGACAAATACTTGGACTTCTTTGCTTGCTATCTGTCCACTTCCTCTGGGCCTGTGAATGGGACTTTGTACATATCCACAAAAAGGGTGGCCTTTTGTAGTGAGTTTCCTCTTTGTTACTATCCATCTCCTGGCGAGCCACAGTGGATCCTTTACAAGGTATTTCTCACCCTTTGTTGAAGATATTTTGTTCTGATGGATTTGAAATAGGATTTTTAAGAAATAAAACTTAGCTGTTTACTGATAAGTAGTTCATTGAGTTTATTTAAGACCGGTTCGATAATCATTTGGCTTTGGATTCTCAAGTTGTGTTTGATTTTACTATTCAAGTGTTTAATTTTGAAAATAAGTCATTTTATAAAAAAATAATAATAATAAAGTATTTGGTAACCATTTAAAATAGTTTTTGAAATGTTTTCGAAGTTTATTTTAAACAATTTTTATCAAAAGAGTTTAAATAAATATGACTCTTTCTGAAAAACATCTTTTTTCTCTAGTCAATCCATATGGGCCCTTAGTTTTTTTAAAAATTAAGCTTTTAAACACTTTTTCGACCTATGGAGTTTCTTTGTTTTGGTATCTACTTCATAAGAATGTTCTCAAAATTCATGTCAAGTTTTGAAGACTAGAAAAAAGTAGGCTCTGTTTGATAACTATATATATATTTTTTTATTTTAATTTTCAAAATTTAACTGGTTAAAAAATCATCAGTAAAAGTAGTGTTTGTAAGCATAATTTTAAAAAATAGAAAACAAAGACAAAACGGTTTTCAACTTTTTTATACTTTCTGAATTTAGCTAGGAATTCAAATGTTACTTTAAAAAAAGATGAAAATCATTATAAAGAAATTGGGAGAAAACAATCCCAGTCCGACCGAACAAGGCCTTAGATATATTGAAGGAAAGACAAAACAGAACGGATACAAGACTTCTCATATGTTTGATGAGAGGTCTGAGACACTTCACAAAAGATTCTACTGACTTAACTCTCTCTATTAACGACCCCACTCACATATACTTCTCTTATCTAACACGTGCGAGGGCCCTGCTGCCTTCCCTCTCTAAGATACTCTATTACAGTTACCAATTCTTTCTCCCTCTAACTTCCATTTGTATTTCTCACCCTTTCTTCTTCGTACACGTGATATGCATAGGTGGCTTCTTGAGTGAAAGAAAAATTTTAGTGCTATGGTACCTCAGCTCATTGTTTGTCATAGAAGAAGCATTTAAAAATGGTGGGTCCATGAAATAAAGTAATATATCATTTAGTGATGTCTTGAATGTTGAATGGAAGGAGTATGTACTACTGTAGTACTATACATTTCTGCTTTTCTTCTCTTAGTTTACGACGAGAGACACAAACAAGAAGAAATAGTTATTAATAGTTGGTTGCCAAGTTTTCTATAAAATTTTTCTATAAACTTGCTTTAGTATGATTATGTTTAATGTTTTGATAGGAAAGGTATTAGCGGGATACCAAGGGTATATTCATAATTAGTTAAGTAGTTTATAAGGAAGGTGTAGTTATAAATATAGTGAGCAGGGAAGGGAGGAAGGAGGAAATACTATGGTGAGTGTGTTAGGACTTAAAGAGTGATCTCAAGAGATTGGGAGGGTCCAAGTACCTCGAATTACTTGGTTTATCTTATAGTTTCATTACCAATCAAATTCAATATATATTTAGGTTCTATCATGTTTTTCCGAATTTAAAGCATGGTGGATCTGCAGTTTGGCAAATAACTATATGATGTACTGCAACAGTTTAATTTTGATCCAATGAATCACAATTTCACTAGGTTTCTCCTGTGAAATTCAATATGAAAATTTTTCCAATTGTTTTTTTTCCTTATCGTGGTAGAAAATAATTGAAGTTCAAATCCATAAGATTAAAATTTAAAAGGCATAATATAAATAGAAATATCTAGTTTTATAGTATAGTTCATAATCTCTCAATGGAGTAAAATAGTCACTCTCCTTCACTGTTTAATGAATGAAGAGATAGTGTATTGGGACTATACTCTTCACTAGGAAAGTATGTGAGCCCATAATTTAATACCATTTAATATACTCATTAAATAGCCTAACATAATTGTCCTTTATTTATTAGCCACTATGAATTAATTTAATAAACTGGGCACCATGGAGTCACTAGCAATGGGTGGAGGAACTGGAAATGCATGAATGCAGGGTCTGATCTTCCTGTAGGACACCATTGATGGATTGTCAGTCCACAGAAACTATAAGCTGTTGTCCAAGAACCTGTTTCTGGCTAGAAGAATAACTTTCTTGGACATCCATCAATTAATATATCCAGAATAGCCCCCCACCCCCCAATCACAATTTTAAAAGATCCACAAAGCAATTGTGTTCCCGCCCACGAACAAGTCAACTGATTCAAATTCAAATAGCCAATGACACAACGGGCGTATCTCGCAACCTCTACCCCGTTTAAAGAAAATTGTTTTAGAAAACGGGGTTTATGAAAAAGGTTTTTGCAAATGTTTGAAAACATGTTTGAGAAAATGGTTATCGTAGGCTAGAAAGCAATGAGAAACAACAACGGCTTAGTAGCTTACAACTCCATGTATGAAGGTAATCTTCCTCTGGCATCTCTAGATGATTATTGAAAAGTAAGTTTAGTGAAATTTGTTCTTCCTCCTAGTTTGGTTTGTTATCGCGTCTGTGCATAAGAGAGTTTATGAAACTATGCTGAAGTCAATGTTTGCATTAAGAATCTCCAATCTCCTATCTCCATCTTTGTGTTTCCATGAAGTATAGTTTAATGGCCCAAATCTGATCTTGAAAACTATGCTTGTGTTAGTATCTAAAATAATTGGTAGTTATGTAGATTCAACAGGATTAATTAGAAAAACGAATCGCCGAGTCTCGTCGAGTTTCTTTGTCCCTTTATCTATCTTTTTCGCTCGTGTTTTCTTCTGTTAATGTCTTTAGTTGCATTCTTTTTCATGACTATGATGAATATGTATGTAGGTGATGATAGCGGTTGATCAGTTGGGGAAAATCAATGCTTCTTCAAACCTTATGGACCCCTCACAAAAATACATCCAAGTAATCACGAGGGACTCTCATGAATTCTGGTTCATGGGATTCATATCATATAACAAGGCTGTCAAGACTTTAACTGATGCATTGCAGCGATCCCGTGCCTAG

mRNA sequence

ATGATCAATCACCTTGACACCAAAGACAATCCCTATGTTCATTATTCCTCTGCTTCCACATCTCCAAATGGGAAATCAGATAAGATGTCGGAAGTGTTGGATCGGTGTGGGAAGAGGTTTGCAGATTGTTCTAAGATGGTAGAGGCTGCAGCAGATGGCATCTGGAATCACTTGAAACTTAGTTCTAGTGTCACTGATGCGGCCATGGCCAGGCTTCATCAAGGAACGAAGTTAGTTACAGAAGGTGGCTATAAAAAAGTATTCCAACAGACGTTTGGTTTCGTCGACGGAGACAAATACTTGGACTTCTTTGCTTGCTATCTGTCCACTTCCTCTGGGCCTGTGAATGGGACTTTGTACATATCCACAAAAAGGGTGGCCTTTTGTAGTGAGTTTCCTCTTTGTTACTATCCATCTCCTGGCGAGCCACAGTGGATCCTTTACAAGGTGATGATAGCGGTTGATCAGTTGGGGAAAATCAATGCTTCTTCAAACCTTATGGACCCCTCACAAAAATACATCCAAGTAATCACGAGGGACTCTCATGAATTCTGGTTCATGGGATTCATATCATATAACAAGGCTGTCAAGACTTTAACTGATGCATTGCAGCGATCCCGTGCCTAG

Coding sequence (CDS)

ATGATCAATCACCTTGACACCAAAGACAATCCCTATGTTCATTATTCCTCTGCTTCCACATCTCCAAATGGGAAATCAGATAAGATGTCGGAAGTGTTGGATCGGTGTGGGAAGAGGTTTGCAGATTGTTCTAAGATGGTAGAGGCTGCAGCAGATGGCATCTGGAATCACTTGAAACTTAGTTCTAGTGTCACTGATGCGGCCATGGCCAGGCTTCATCAAGGAACGAAGTTAGTTACAGAAGGTGGCTATAAAAAAGTATTCCAACAGACGTTTGGTTTCGTCGACGGAGACAAATACTTGGACTTCTTTGCTTGCTATCTGTCCACTTCCTCTGGGCCTGTGAATGGGACTTTGTACATATCCACAAAAAGGGTGGCCTTTTGTAGTGAGTTTCCTCTTTGTTACTATCCATCTCCTGGCGAGCCACAGTGGATCCTTTACAAGGTGATGATAGCGGTTGATCAGTTGGGGAAAATCAATGCTTCTTCAAACCTTATGGACCCCTCACAAAAATACATCCAAGTAATCACGAGGGACTCTCATGAATTCTGGTTCATGGGATTCATATCATATAACAAGGCTGTCAAGACTTTAACTGATGCATTGCAGCGATCCCGTGCCTAG

Protein sequence

MINHLDTKDNPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRVAFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWFMGFISYNKAVKTLTDALQRSRA
Homology
BLAST of Bhi05G000035 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 197.2 bits (500), Expect = 1.3e-50
Identity = 96/207 (46.38%), Postives = 143/207 (69.08%), Query Frame = 0

Query: 3   NHLDTKDNPYVHYSSASTSPNGKS-DKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLS 62
           +H    DNPYVH +S +++ + +S DK+ EVL+RCGK+  D ++  EA   G+ +HLK S
Sbjct: 22  SHGGRYDNPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFS 81

Query: 63  SSVTDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYI 122
            S++DAAMARL QGTK++ EGG ++VFQ+ FG +  +K LD F CY+ST+SGPV G +YI
Sbjct: 82  PSISDAAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYI 141

Query: 123 STKRVAFCSEFPLCYYPSP-GEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRD 182
           S +R+AFCS++ +    S  G      YKV++  +++  I++S+N++ PS++Y+ ++TRD
Sbjct: 142 SNRRIAFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRD 201

Query: 183 SHEFWFMGFISYNKAVKTLTDALQRSR 208
             EFWFMGF+SY  A   L  AL  SR
Sbjct: 202 GFEFWFMGFVSYIDAFNCLNKALLNSR 228

BLAST of Bhi05G000035 vs. TAIR 10
Match: AT2G22475.1 (GRAM domain family protein )

HSP 1 Score: 191.0 bits (484), Expect = 9.3e-49
Identity = 95/198 (47.98%), Postives = 135/198 (68.18%), Query Frame = 0

Query: 10  NPYVHYSSASTSPNGKSDKMSE---VLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTD 69
           NPY+  S A TS     D M     VL R GKR A+ +K  E+ A   W HL+ + S  D
Sbjct: 96  NPYIARSPAETSDASLKDTMETVKGVLGRWGKRVAEAAKKTESLAGNTWQHLRTAPSFAD 155

Query: 70  AAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRV 129
           AAM R+ Q TK+  EGGY+K+F+QTF     ++ L+ FACYLSTS+GPV G LYIS+ ++
Sbjct: 156 AAMGRIAQSTKVFAEGGYEKIFRQTFETDPEEQLLNSFACYLSTSAGPVMGVLYISSAKL 215

Query: 130 AFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWF 189
           A+CS+ PL  Y +  + +W  YKV+I + QL  +N S+++++P++KYIQVI+ D+HEFWF
Sbjct: 216 AYCSDNPLS-YKNGDQTEWSYYKVVIPLHQLKAVNPSASIVNPAEKYIQVISVDNHEFWF 275

Query: 190 MGFISYNKAVKTLTDALQ 205
           MGF++Y+ AV +L D+LQ
Sbjct: 276 MGFLNYDGAVTSLQDSLQ 292

BLAST of Bhi05G000035 vs. TAIR 10
Match: AT1G28200.1 (FH interacting protein 1 )

HSP 1 Score: 188.7 bits (478), Expect = 4.6e-48
Identity = 93/200 (46.50%), Postives = 132/200 (66.00%), Query Frame = 0

Query: 5   LDTKDNPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLSSSV 64
           +  + NPYV   S S +P    D + + L + GK  AD +K  E  A   W HLK   SV
Sbjct: 60  MPAESNPYV---SPSPAPRNTMDSVKDTLGKWGKMAADATKKAEDLAGNFWQHLKTGPSV 119

Query: 65  TDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTK 124
            DAA++R+ QGTK++ EGGY+KVF+QTF  +  +K L  +ACYLSTS+GPV G +Y+ST 
Sbjct: 120 ADAAVSRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKTYACYLSTSAGPVLGVMYLSTH 179

Query: 125 RVAFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEF 184
           ++AF S+ PL Y     +  W  YKV++  +QL  +N S++ ++ S KYIQVI+ D+HEF
Sbjct: 180 KLAFSSDNPLSYKEGE-QTLWSYYKVVLPANQLKAVNPSTSRVNTSDKYIQVISIDNHEF 239

Query: 185 WFMGFISYNKAVKTLTDALQ 205
           WFMGF++Y  AVK+L +A+Q
Sbjct: 240 WFMGFVTYESAVKSLQEAVQ 255

BLAST of Bhi05G000035 vs. TAIR 10
Match: AT4G01600.2 (GRAM domain family protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.1e-46
Identity = 93/207 (44.93%), Postives = 139/207 (67.15%), Query Frame = 0

Query: 3   NHLDTKDNPYVHYSSASTSPNGKS-DKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLS 62
           +H    DNPYVH +S +++ + +S DK+ EVL+RCGK+  D ++  EA        +K S
Sbjct: 22  SHGGRYDNPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVG-----VKFS 81

Query: 63  SSVTDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYI 122
            S++DAAMARL QGTK++ EGG ++VFQ+ FG +  +K LD F CY+ST+SGPV G +YI
Sbjct: 82  PSISDAAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYI 141

Query: 123 STKRVAFCSEFPLCYYPSP-GEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRD 182
           S +R+AFCS++ +    S  G      YKV++  +++  I++S+N++ PS++Y+ ++TRD
Sbjct: 142 SNRRIAFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRD 201

Query: 183 SHEFWFMGFISYNKAVKTLTDALQRSR 208
             EFWFMGF+SY  A   L  AL  SR
Sbjct: 202 GFEFWFMGFVSYIDAFNCLNKALLNSR 223

BLAST of Bhi05G000035 vs. TAIR 10
Match: AT5G13200.1 (GRAM domain family protein )

HSP 1 Score: 137.9 bits (346), Expect = 9.3e-33
Identity = 69/193 (35.75%), Postives = 108/193 (55.96%), Query Frame = 0

Query: 7   TKDNPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTD 66
           T+  PYV YS        +    +  L+     F   S+  E  A  +W++LK   S+++
Sbjct: 70  TQYQPYVIYSPV------EHPTTNNPLEPVIGMFHTWSRKAETVARNLWHNLKTGPSMSE 129

Query: 67  AAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRV 126
            A  +++   K +T+GG++ +F+Q FG    +     FACYLST++GPV GT+Y+S  RV
Sbjct: 130 TAWGKVNLTAKAITKGGFESLFRQIFGTEPNETLKKTFACYLSTTTGPVAGTVYLSNARV 189

Query: 127 AFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWF 186
           AFCS+ PL +    G+  W  Y+V++ +  +  +N       P +KYIQ+ T D H+FWF
Sbjct: 190 AFCSDRPLYFTAPSGQESWSYYRVVVPLANVATVNPVVVKETPPEKYIQLTTVDGHDFWF 249

Query: 187 MGFISYNKAVKTL 200
           MGF++Y KA   L
Sbjct: 250 MGFVNYEKATHHL 256

BLAST of Bhi05G000035 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.8e-49
Identity = 96/207 (46.38%), Postives = 143/207 (69.08%), Query Frame = 0

Query: 3   NHLDTKDNPYVHYSSASTSPNGKS-DKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLS 62
           +H    DNPYVH +S +++ + +S DK+ EVL+RCGK+  D ++  EA   G+ +HLK S
Sbjct: 22  SHGGRYDNPYVHITSPTSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFS 81

Query: 63  SSVTDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYI 122
            S++DAAMARL QGTK++ EGG ++VFQ+ FG +  +K LD F CY+ST+SGPV G +YI
Sbjct: 82  PSISDAAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYI 141

Query: 123 STKRVAFCSEFPLCYYPSP-GEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRD 182
           S +R+AFCS++ +    S  G      YKV++  +++  I++S+N++ PS++Y+ ++TRD
Sbjct: 142 SNRRIAFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRD 201

Query: 183 SHEFWFMGFISYNKAVKTLTDALQRSR 208
             EFWFMGF+SY  A   L  AL  SR
Sbjct: 202 GFEFWFMGFVSYIDAFNCLNKALLNSR 228

BLAST of Bhi05G000035 vs. ExPASy Swiss-Prot
Match: Q8S8F8 (GLABRA2 expression modulator OS=Arabidopsis thaliana OX=3702 GN=GEM PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-47
Identity = 95/198 (47.98%), Postives = 135/198 (68.18%), Query Frame = 0

Query: 10  NPYVHYSSASTSPNGKSDKMSE---VLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTD 69
           NPY+  S A TS     D M     VL R GKR A+ +K  E+ A   W HL+ + S  D
Sbjct: 96  NPYIARSPAETSDASLKDTMETVKGVLGRWGKRVAEAAKKTESLAGNTWQHLRTAPSFAD 155

Query: 70  AAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRV 129
           AAM R+ Q TK+  EGGY+K+F+QTF     ++ L+ FACYLSTS+GPV G LYIS+ ++
Sbjct: 156 AAMGRIAQSTKVFAEGGYEKIFRQTFETDPEEQLLNSFACYLSTSAGPVMGVLYISSAKL 215

Query: 130 AFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWF 189
           A+CS+ PL  Y +  + +W  YKV+I + QL  +N S+++++P++KYIQVI+ D+HEFWF
Sbjct: 216 AYCSDNPLS-YKNGDQTEWSYYKVVIPLHQLKAVNPSASIVNPAEKYIQVISVDNHEFWF 275

Query: 190 MGFISYNKAVKTLTDALQ 205
           MGF++Y+ AV +L D+LQ
Sbjct: 276 MGFLNYDGAVTSLQDSLQ 292

BLAST of Bhi05G000035 vs. ExPASy Swiss-Prot
Match: Q9SE96 (GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 6.5e-47
Identity = 93/200 (46.50%), Postives = 132/200 (66.00%), Query Frame = 0

Query: 5   LDTKDNPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLSSSV 64
           +  + NPYV   S S +P    D + + L + GK  AD +K  E  A   W HLK   SV
Sbjct: 60  MPAESNPYV---SPSPAPRNTMDSVKDTLGKWGKMAADATKKAEDLAGNFWQHLKTGPSV 119

Query: 65  TDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTK 124
            DAA++R+ QGTK++ EGGY+KVF+QTF  +  +K L  +ACYLSTS+GPV G +Y+ST 
Sbjct: 120 ADAAVSRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKTYACYLSTSAGPVLGVMYLSTH 179

Query: 125 RVAFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEF 184
           ++AF S+ PL Y     +  W  YKV++  +QL  +N S++ ++ S KYIQVI+ D+HEF
Sbjct: 180 KLAFSSDNPLSYKEGE-QTLWSYYKVVLPANQLKAVNPSTSRVNTSDKYIQVISIDNHEF 239

Query: 185 WFMGFISYNKAVKTLTDALQ 205
           WFMGF++Y  AVK+L +A+Q
Sbjct: 240 WFMGFVTYESAVKSLQEAVQ 255

BLAST of Bhi05G000035 vs. ExPASy Swiss-Prot
Match: Q9M063 (Putative GEM-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g40100 PE=3 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 2.6e-35
Identity = 82/200 (41.00%), Postives = 116/200 (58.00%), Query Frame = 0

Query: 8   KDNPYVHYSSASTSPNGKSDKMSE---VLDRCGKRFADCSKMVEAAADGIWNHLKLSSSV 67
           + NPYV  +   TS     + M     VL R G+R  + +   E+ A   W H       
Sbjct: 53  RSNPYVARAPTETSDASLKETMESVKGVLGRWGRRVGEAAMKAESLAGNTWQH------P 112

Query: 68  TDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTK 127
             AAM R+ Q TK++ EGGY+K+F+QTF  V  ++  + FACYLSTS+GPV G LY+ST 
Sbjct: 113 LRAAMGRIAQSTKVLAEGGYEKIFRQTFETVPEEQLQNSFACYLSTSAGPVMGVLYVSTA 172

Query: 128 RVAFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEF 187
           ++A+CS+  L               V+I + QL  +N S + ++P++KYIQVI+ D HEF
Sbjct: 173 KLAYCSDTSL---------------VVIPLHQLKSVNPSISTVNPAEKYIQVISVDDHEF 231

Query: 188 WFMGFISYNKAVKTLTDALQ 205
           WFMGF++Y  AV +L D LQ
Sbjct: 233 WFMGFLNYEGAVTSLQDTLQ 231

BLAST of Bhi05G000035 vs. ExPASy Swiss-Prot
Match: Q9LYV6 (GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.3e-31
Identity = 69/193 (35.75%), Postives = 108/193 (55.96%), Query Frame = 0

Query: 7   TKDNPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTD 66
           T+  PYV YS        +    +  L+     F   S+  E  A  +W++LK   S+++
Sbjct: 70  TQYQPYVIYSPV------EHPTTNNPLEPVIGMFHTWSRKAETVARNLWHNLKTGPSMSE 129

Query: 67  AAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRV 126
            A  +++   K +T+GG++ +F+Q FG    +     FACYLST++GPV GT+Y+S  RV
Sbjct: 130 TAWGKVNLTAKAITKGGFESLFRQIFGTEPNETLKKTFACYLSTTTGPVAGTVYLSNARV 189

Query: 127 AFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWF 186
           AFCS+ PL +    G+  W  Y+V++ +  +  +N       P +KYIQ+ T D H+FWF
Sbjct: 190 AFCSDRPLYFTAPSGQESWSYYRVVVPLANVATVNPVVVKETPPEKYIQLTTVDGHDFWF 249

Query: 187 MGFISYNKAVKTL 200
           MGF++Y KA   L
Sbjct: 250 MGFVNYEKATHHL 256

BLAST of Bhi05G000035 vs. ExPASy TrEMBL
Match: A0A1S3CCD7 (GEM-like protein 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499226 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.2e-97
Identity = 178/208 (85.58%), Postives = 192/208 (92.31%), Query Frame = 0

Query: 1   MINHLDTKDNPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKL 60
           M N LD+++N YV  SSASTSPNGK  KM EVLDRCGK+F DCSK VEAAADG+WNH+KL
Sbjct: 7   MSNCLDSQENHYVQCSSASTSPNGKY-KMWEVLDRCGKKFEDCSKTVEAAADGVWNHMKL 66

Query: 61  SSSVTDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLY 120
           SSSVTDAAMARLHQGTKL+TEGGYKKVFQQTFGFVDGDKYLD FACYLSTSSGPVNGTLY
Sbjct: 67  SSSVTDAAMARLHQGTKLLTEGGYKKVFQQTFGFVDGDKYLDCFACYLSTSSGPVNGTLY 126

Query: 121 ISTKRVAFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRD 180
           ISTKRVAFCS FPLCYYPSPG+PQWILYK++IAVDQ+ KINASSN MDPSQKYIQ+IT D
Sbjct: 127 ISTKRVAFCSAFPLCYYPSPGQPQWILYKMVIAVDQVEKINASSNPMDPSQKYIQLITWD 186

Query: 181 SHEFWFMGFISYNKAVKTLTDALQRSRA 209
           SHEFWFMGFISYNKA+KTLT+ LQRSRA
Sbjct: 187 SHEFWFMGFISYNKALKTLTNTLQRSRA 213

BLAST of Bhi05G000035 vs. ExPASy TrEMBL
Match: A0A1S3CCH4 (GEM-like protein 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499226 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 8.7e-95
Identity = 179/237 (75.53%), Postives = 193/237 (81.43%), Query Frame = 0

Query: 1   MINHLDTKDNPYVHYSSASTSPNGKS-----------------------------DKMSE 60
           M N LD+++N YV  SSASTSPNGK                              DKM E
Sbjct: 7   MSNCLDSQENHYVQCSSASTSPNGKCLLFSTLNVFVKTPQWYLLPFFFFFVICPIDKMWE 66

Query: 61  VLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTDAAMARLHQGTKLVTEGGYKKVFQQT 120
           VLDRCGK+F DCSK VEAAADG+WNH+KLSSSVTDAAMARLHQGTKL+TEGGYKKVFQQT
Sbjct: 67  VLDRCGKKFEDCSKTVEAAADGVWNHMKLSSSVTDAAMARLHQGTKLLTEGGYKKVFQQT 126

Query: 121 FGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRVAFCSEFPLCYYPSPGEPQWILYKVM 180
           FGFVDGDKYLD FACYLSTSSGPVNGTLYISTKRVAFCS FPLCYYPSPG+PQWILYK++
Sbjct: 127 FGFVDGDKYLDCFACYLSTSSGPVNGTLYISTKRVAFCSAFPLCYYPSPGQPQWILYKMV 186

Query: 181 IAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWFMGFISYNKAVKTLTDALQRSRA 209
           IAVDQ+ KINASSN MDPSQKYIQ+IT DSHEFWFMGFISYNKA+KTLT+ LQRSRA
Sbjct: 187 IAVDQVEKINASSNPMDPSQKYIQLITWDSHEFWFMGFISYNKALKTLTNTLQRSRA 243

BLAST of Bhi05G000035 vs. ExPASy TrEMBL
Match: A0A6J1E018 (GEM-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111026148 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.2e-91
Identity = 167/208 (80.29%), Postives = 184/208 (88.46%), Query Frame = 0

Query: 1   MINHLDTKDNPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKL 60
           M N LD ++NPYV +SSAS SPNGK +KM EVL RCGKR  DCS+ VE AAD + +HL+L
Sbjct: 1   MSNRLDDRENPYVQFSSASASPNGK-NKMWEVLGRCGKRLEDCSRKVEVAADSVRHHLRL 60

Query: 61  SSSVTDAAMARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLY 120
           S SVTDAAMARLHQGTKL+TEGGY+KVFQQTFGF+ GDKY+D FACYLSTSSGPVNGTLY
Sbjct: 61  SPSVTDAAMARLHQGTKLLTEGGYEKVFQQTFGFIHGDKYVDSFACYLSTSSGPVNGTLY 120

Query: 121 ISTKRVAFCSEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRD 180
           ISTKRVAFCSEFPLCYYPSPG+ QWI YKV++AVDQLG IN SSN +DPSQKYIQVITRD
Sbjct: 121 ISTKRVAFCSEFPLCYYPSPGQQQWIHYKVVVAVDQLGTINTSSNHLDPSQKYIQVITRD 180

Query: 181 SHEFWFMGFISYNKAVKTLTDALQRSRA 209
            HEFWFMGFISYNKAVKTLTDA Q+SRA
Sbjct: 181 XHEFWFMGFISYNKAVKTLTDAFQQSRA 207

BLAST of Bhi05G000035 vs. ExPASy TrEMBL
Match: A0A1S3CCV4 (GEM-like protein 2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103499226 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 8.4e-90
Identity = 161/181 (88.95%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 28  KMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTDAAMARLHQGTKLVTEGGYKKV 87
           KM EVLDRCGK+F DCSK VEAAADG+WNH+KLSSSVTDAAMARLHQGTKL+TEGGYKKV
Sbjct: 9   KMWEVLDRCGKKFEDCSKTVEAAADGVWNHMKLSSSVTDAAMARLHQGTKLLTEGGYKKV 68

Query: 88  FQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRVAFCSEFPLCYYPSPGEPQWIL 147
           FQQTFGFVDGDKYLD FACYLSTSSGPVNGTLYISTKRVAFCS FPLCYYPSPG+PQWIL
Sbjct: 69  FQQTFGFVDGDKYLDCFACYLSTSSGPVNGTLYISTKRVAFCSAFPLCYYPSPGQPQWIL 128

Query: 148 YKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWFMGFISYNKAVKTLTDALQRSR 207
           YK++IAVDQ+ KINASSN MDPSQKYIQ+IT DSHEFWFMGFISYNKA+KTLT+ LQRSR
Sbjct: 129 YKMVIAVDQVEKINASSNPMDPSQKYIQLITWDSHEFWFMGFISYNKALKTLTNTLQRSR 188

Query: 208 A 209
           A
Sbjct: 189 A 189

BLAST of Bhi05G000035 vs. ExPASy TrEMBL
Match: A0A6J1DUB7 (GEM-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111023171 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 6.7e-63
Identity = 116/199 (58.29%), Postives = 153/199 (76.88%), Query Frame = 0

Query: 10  NPYVHYSSASTSPNGKSDKMSEVLDRCGKRFADCSKMVEAAADGIWNHLKLSSSVTDAAM 69
           NPY+ Y +++ +  GK DK+   L RCGK   +  K+ E AAD + +H+K+S S+ DAA 
Sbjct: 10  NPYIQYCASAPAQPGKKDKIRGALGRCGKMLEEYGKIAEEAADDVLHHIKVSPSIGDAAK 69

Query: 70  ARLHQGTKLVTEGGYKKVFQQTFGFVDGDKYLDFFACYLSTSSGPVNGTLYISTKRVAFC 129
           ARL QGTKLVTEGG +K+FQ TFG + GDKYL  +ACYLSTSSGPVNGTLYISTKRVAF 
Sbjct: 70  ARLIQGTKLVTEGGPEKLFQHTFGVIPGDKYLHSYACYLSTSSGPVNGTLYISTKRVAFG 129

Query: 130 SEFPLCYYPSPGEPQWILYKVMIAVDQLGKINASSNLMDPSQKYIQVITRDSHEFWFMGF 189
           SE PLCY  SPG+P+W+ YKV+I ++Q+  IN S NL++PS+K I ++TRD HEFWFMGF
Sbjct: 130 SESPLCYSSSPGQPEWMYYKVVIELNQVAMINPSPNLLNPSEKDIHIVTRDGHEFWFMGF 189

Query: 190 ISYNKAVKTLTDALQRSRA 209
           I +++A+K+L +AL+ + A
Sbjct: 190 ICFSRALKSLNEALKHAHA 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G01600.11.3e-5046.38GRAM domain family protein [more]
AT2G22475.19.3e-4947.98GRAM domain family protein [more]
AT1G28200.14.6e-4846.50FH interacting protein 1 [more]
AT4G01600.21.1e-4644.93GRAM domain family protein [more]
AT5G13200.19.3e-3335.75GRAM domain family protein [more]
Match NameE-valueIdentityDescription
Q9M1221.8e-4946.38GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1[more]
Q8S8F81.3e-4747.98GLABRA2 expression modulator OS=Arabidopsis thaliana OX=3702 GN=GEM PE=1 SV=1[more]
Q9SE966.5e-4746.50GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1[more]
Q9M0632.6e-3541.00Putative GEM-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g40100 PE=3 SV... [more]
Q9LYV61.3e-3135.75GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CCD73.2e-9785.58GEM-like protein 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499226 PE=3 SV=1[more]
A0A1S3CCH48.7e-9575.53GEM-like protein 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499226 PE=3 SV=1[more]
A0A6J1E0181.2e-9180.29GEM-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111026148 PE=3 SV=1[more]
A0A1S3CCV48.4e-9088.95GEM-like protein 2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103499226 PE=3 SV=1[more]
A0A6J1DUB76.7e-6358.29GEM-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111023171 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 86..164
e-value: 2.8E-10
score: 50.2
IPR004182GRAM domainPFAMPF02893GRAMcoord: 86..205
e-value: 7.4E-19
score: 67.7
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 84..205
e-value: 2.3E-19
score: 71.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..27
NoneNo IPR availablePANTHERPTHR31969:SF9GEM-LIKE PROTEIN 2coord: 8..206
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 8..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000035Bhi05M000035mRNA