Bhi05G000002 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G000002
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	4-coumarate-CoA ligase
Location	chr5: 143523 .. 148611 (-)
RNA-Seq Expression	Bhi05G000002
Synteny	Bhi05G000002

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACCGCTATTAAACGATCACTTCAGTTATCAAACGCCAGAAAATGAGACTGTTATCACTAAAAAAATTCCTCGGTCTGAACAAACCAACAAATATCCTAATTGGTATTCACCAGATACAGGAATTTGCCGTAGTGTTCATGCTTCTAGAGGAGTTCCCTCTGATCCATTTCTTGATGTTGTCTCCTTCATTTTCTCCTTCAAACACAATGGAAATTCAGCTATCATTGATTCCTCAACTGGGAGTTCAATATCTTACAAAGAAATGTTTCATATGGTCAAATCCATGGCTTCTGGACTCCAAAAATTCGGTATATCACAAGGGGATGTAGTTTTGCTTGTGTTACCAAATTCCATTTTCTATCCCATTATTCTTTTAGGTGTTCTGTATTTAGGAGCAGTTGTTACAACCATGTTTCCTCAAAGCAGTTCTTCAGAAATCAAGAAACGAATCAATGATTGCAATGTTCGATTGGCTTTTACAATAACTCAGAAAATCAAGAATTTTGAGTCATTGGGAATTCAGGCAATTGGGGTACCAGAAATTACCAATTTCGACTTGATGCAGCCTATGGGATTTTCTTATTTTCATGAGCTTATTTCAAGTGGTTCTGATTTGAAACAAAGACCAGTGATTAGACAAGAAGATACAGCTGCTCTATTGTTTTCTTCAGGTACTACTGGAGTAAGCAAAGGGGTTATGTTAACACATAGAAATTTTATATCTACGATCGAGCTTTTTGTTCGGTTTGAAGCTTCACAGTACGAATATTTGCCTACAGAGAATGTGTATTTGGCTGCTATACCAATGTTTCATATATATGGTCTTTCCATTTTTGTGATGGGATTACTGTCATTGGGATCTAGTGTTGTTATAATGAGCAAATTTGATGTGAAAGAGGTAGTCAAGGCTATTGACAGATTTAAAGTCACACATTTTCCAGTTGTTCCACCTATAATGACAGCTATGGCTAGAACTGCAGAGAAAATTGGTGTGCATAGATTTAGAAGTTTGAAACAAGTTTCTTGTGGAGCTGCTTCTTTGAACAAGAAGACCATTGATGATTTTGTTAAGGCTTTCCCTCATGTTGACTTCATTCAGGTATTCTACTTGACACTTATATTACATTAAACTTAAGAAACACGGACACTGTAGTTTGGCTAGTGTGTCCATGTTCTGACACATGATGGATACTTAGATACTTTAACACTAGTTGGAGATATATCAAACACTTGTTAGTTCAATAAATATGTTAGATCTATGCATAGAACACTTGTTGAGTACACTAAAAATAAACAAAAAAGACACATAAATAATAATAAATTTTGAGTGTGTAATACATCAAACTAATTTTTTAAGCATTTAAATGCATCAATGCACTTACTTTGAATTAACATTTAAATGCATCAACCAACTTTGAATTTTCTTCTAATATAAAAAGGATATATATTTTAATAGACGTATCCTTATTATGTTGTATCCTAGATTATTAAAAAGCAATGTGCCCTTGTGTCTATGTCGTGTCGTGTCCGATGTCTGTATCAAAGCATCAAAAGCAATGTTTTGAGGAATTTTAGCTCGTAATGTTGATGGGCCAAGTTGTTAATTTGAACAATGCTTGGTTTCGGTGTATTTGTTGTTAGGGATATGGCATGACTGAGTCCACAGCTATAGGAACTCGCGGCTTCAATACCAAAAATGCTAGAAATTATACGTCAGTTGGACTTTTAGCTCCAAACACAGAAGCTAAAGTAGTTGATTGGGTTTCCGGATCTTTCATGCCTCCTGGTAAAACCGGCGAACTTTTGTTACGCGGACCCGGTTCAATGAAAGGTTTGAAGGATTCTTTCTAAAACAATCCTTGCATTGCCCTAAGATCTTGTTAAACTACCATTAACTCAAAAGTTTATATTGATAGGTTACGATATACTTACGACTTTTATTTTTAATTCGGTAAGCAAAATGATGATCAATATTAATGGAGGAAATGACATGATAGGATTCGGACTCTAGACTTTATGACTTGAAACCATTTCAAACTACTGCTGAAATCAAAGGTTTAAGTTGATAGGTTAATACTTTATTTCTATTCTTAACCTATCTATTTGTTGGCTTTAGTGAATTTCACATCACTCTTATCTGCTTCACTTCTGTTATAAATCCTCAATTTGAAAAAATTTTATTCTGTAAATCTGGACGAAACTATGTCTGTCATTTTCTTTATAGATCAAACTCAGCCTATGAAGTTGGAAAGCTTCTTTTGATGTATTGTTTTTTTTGGTTTTTTTTTTTTTTTTGGCCTTTACAGGGTACTTGAATAATCCAGAGGCAACAATGTCCACAATAGATAAAGAAAATTGGCTACATACTGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTATTGATCGTTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGGTACAATATAATAAGGGACACATTCATGTTTTTTACACATCCAAATGGTTACCAACGACGCCTAGAGTGAAATACTTTTGTTTTTAGCTTCGCAATATAAGCACTATATAACTGGCGTTAACTCTTTAGCCAAGAAAGTAAGTTAGAATCTAAAAAAAATTCAATTTCTTAGCCTTTGTTAAACATTCGGGTTCTATTTGGTAATCATTTGGTTTTTTGTTTTTGAAAGTTAAGCATATTCCTCTCTATTTCCTACCATGATTTTCATCTTCTTAAGTAAAAGAGTTGAATTATTAGTCAAATTACAAAAACAAAAACAAGTTTTTAAAAACTATTTTTAGTTTTCAAAACTTGGCTTGGTTTTTAAAAACATTGGTAGAAAGTAGATAATAAAACAAGAAATTTAAATATGTAATGTATATCTATAGGCTTAATTTTTTAAAACTAAAAATCAAATGGTTATCAAATAGGATTTTAATTTTTTTGTTTTTGCATTCTTAGTTTTTGAAAATTAAATCTATATACACTCTTTCTACCTCTAAGTTTTTTGCTTTGTTATCTACATTTTACTAATGTTTTCAAAAAACTAAGCCAAATTTTGGAAACAAAAAATATAGTTTTTAAAAATTTATTTTTGTTTTTAGAATTACTTAAAATAGAGAGGAAAGATGCTTGAATTTCAAAAACCAAAACAAAAAACAAAATGGTTTTCGAGTGAGGTCTTATATATAATTTTAATCTTATAGGAGTTAAGTCTTGTTACTTCTTCACTATACCCCTGCTTATGATGTGTCAATGCTTTGAATGAAGGCCTAGTTTACCACTCTTTAATCAAATTGCTGCAATCATAATAGATTTTTTTTTTATTATTCATAATTCACATAATATTATTAATTAATAATTTACAAATGTATTTAAATGGACACACTTTTTATATTAAGATTGATTGATTACGTTTACAGTAAAACTATCCGAAGTTCATGAGTAATTATGATAGATAAAAGAGTTACACTTGCTTTATCTGTTATTTTGTAGATTGCTCCTACTGATTTAGAAGCTGTGGTGATGACACATCCAGAAGTTCTCGATACTGCGGTAACAGCGTAAGTATTCTTCTAACCATACATTTAGATACAGAGTAAACATCTTTTAGTTAGTTACTCTAATCATTTCAATCAAAGCCAATACACTTGTTAGTTTTTCAATGCATGCGTTAATACATGCAACATTGAGAATGGCACAAGTATTATGTGTTTTGTCTAGTAAAGAAGAAAGGAGTTATCTCCAAAAGTTTCGGATAAATTATGAACTGACTGTCAACTAGGAATTATGTACAAGAAAACGCAGCCAATTCATGAAAAATGTGTTTGTTATTTAATTACCTTTTTAGTCCCTAGGTTTCGAATAATATGTGTGTTTGGTTTTTAAGTTTTAAATATATACTTTTTTAGTCCTCGAATTTTTAAGCATAGGTGCACTTGGTTCATGAGTTTTTAAAATAGACCTTTTTAACCCTTGAATTTGTAAGAATAGATTTTTAGAAGGTTCTTGAAGTACTTAAAAAATATTTTTCTAATTTTGAATATCGAATGATTATTTAAAAATAATTAAAAAAAATACTTCAAGAACCTTCTAAAATAATAATGATTTATTTGATGGACCTTCTAAACCTATTCTTAAAAACTTGATGACTAAAAAGGTACATTTTGAAAACTTAAGGGTCGTTTGGGCCACCGAGTGGAGTCAATATGTCTGGAGTTAATATGTTAAAGTATATTTGTGTTTGATGGGTAGAGTTGTAAGATGAAGTTTTTTGATAAATATGAGAAGTAAAGAAAGAAGAGAATGTGGAATTTCTCGATAATCGATGTGAAAAGTAGAGATAGATGGGAAGATGAAGTTCCTTGTGAAAAGTTGAAAAAGATGGGAAGATGAAATTATTTGATAAATGTGCAAATTTCAATATTGTTTACCATTGAAGTTGAGTTGTTTACACCAATTTAAGAAGTTGGTGGGCCAAATGACCATTAGGGATTAAACACACTTATTCTTAAAAATCTGAAGACTAAAAAGGTATATTTTGAATAGGACCAAATGCACCTATTCTTCGAAACTCGAGGACTAAAAAGTCACATTTTGAAAACCTAACGACACATCTTGAGGTCATGCTTAGAACATGCTTAGTACAACCTGTGTCCATCCATATAAATTCTGATCACCAACCAAGTTAAGTTTTATGCTGTTTTTAAGATTATTTCAGATCATATCTTAGACCCTTTTTTTCCATACTTCGGTTTTTTTCAGGGCCAAAGATGAAGAATGTGGGGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGCAAACTCTCACAGAAAGATGTTATTGATTATGTTGCTCAACAGGTTTCCCATATTTCAAGTCTATGCATTTTTTACCATTAAAAATGTAAAAAAAAAAAAAAAAATTGCCACCTCACATTGAATATCTGGTATCATTTTTGGCTTTTGCTACAGGTTGCACCATATAAAAAAGTAAGAAAAGTGGTTTTTACAGAATCAATACCAAAATCTGCTGCTGGCAAAGTACTTCGTAGAGAACTTGGGAAGCACTTGCCTTCTAAACTGTGA

mRNA sequence

ATGGCACCGCTATTAAACGATCACTTCAGTTATCAAACGCCAGAAAATGAGACTGTTATCACTAAAAAAATTCCTCGGTCTGAACAAACCAACAAATATCCTAATTGGTATTCACCAGATACAGGAATTTGCCGTAGTGTTCATGCTTCTAGAGGAGTTCCCTCTGATCCATTTCTTGATGTTGTCTCCTTCATTTTCTCCTTCAAACACAATGGAAATTCAGCTATCATTGATTCCTCAACTGGGAGTTCAATATCTTACAAAGAAATGTTTCATATGGTCAAATCCATGGCTTCTGGACTCCAAAAATTCGGTATATCACAAGGGGATGTAGTTTTGCTTGTGTTACCAAATTCCATTTTCTATCCCATTATTCTTTTAGGTGTTCTGTATTTAGGAGCAGTTGTTACAACCATGTTTCCTCAAAGCAGTTCTTCAGAAATCAAGAAACGAATCAATGATTGCAATGTTCGATTGGCTTTTACAATAACTCAGAAAATCAAGAATTTTGAGTCATTGGGAATTCAGGCAATTGGGGTACCAGAAATTACCAATTTCGACTTGATGCAGCCTATGGGATTTTCTTATTTTCATGAGCTTATTTCAAGTGGTTCTGATTTGAAACAAAGACCAGTGATTAGACAAGAAGATACAGCTGCTCTATTGTTTTCTTCAGGTACTACTGGAGTAAGCAAAGGGGTTATGTTAACACATAGAAATTTTATATCTACGATCGAGCTTTTTGTTCGGTTTGAAGCTTCACAGTACGAATATTTGCCTACAGAGAATGTGTATTTGGCTGCTATACCAATGTTTCATATATATGGTCTTTCCATTTTTGTGATGGGATTACTGTCATTGGGATCTAGTGTTGTTATAATGAGCAAATTTGATGTGAAAGAGGTAGTCAAGGCTATTGACAGATTTAAAGTCACACATTTTCCAGTTGTTCCACCTATAATGACAGCTATGGCTAGAACTGCAGAGAAAATTGGTGTGCATAGATTTAGAAGTTTGAAACAAGTTTCTTGTGGAGCTGCTTCTTTGAACAAGAAGACCATTGATGATTTTGTTAAGGCTTTCCCTCATGTTGACTTCATTCAGGGATATGGCATGACTGAGTCCACAGCTATAGGAACTCGCGGCTTCAATACCAAAAATGCTAGAAATTATACGTCAGTTGGACTTTTAGCTCCAAACACAGAAGCTAAAGTAGTTGATTGGGTTTCCGGATCTTTCATGCCTCCTGGTAAAACCGGCGAACTTTTGTTACGCGGACCCGGTTCAATGAAAGGGTACTTGAATAATCCAGAGGCAACAATGTCCACAATAGATAAAGAAAATTGGCTACATACTGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTATTGATCGTTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGATTGCTCCTACTGATTTAGAAGCTGTGGTGATGACACATCCAGAAGTTCTCGATACTGCGGTAACAGCGGCCAAAGATGAAGAATGTGGGGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGCAAACTCTCACAGAAAGATGTTATTGATTATGTTGCTCAACAGGTTGCACCATATAAAAAAGTAAGAAAAGTGGTTTTTACAGAATCAATACCAAAATCTGCTGCTGGCAAAGTACTTCGTAGAGAACTTGGGAAGCACTTGCCTTCTAAACTGTGA

Coding sequence (CDS)

ATGGCACCGCTATTAAACGATCACTTCAGTTATCAAACGCCAGAAAATGAGACTGTTATCACTAAAAAAATTCCTCGGTCTGAACAAACCAACAAATATCCTAATTGGTATTCACCAGATACAGGAATTTGCCGTAGTGTTCATGCTTCTAGAGGAGTTCCCTCTGATCCATTTCTTGATGTTGTCTCCTTCATTTTCTCCTTCAAACACAATGGAAATTCAGCTATCATTGATTCCTCAACTGGGAGTTCAATATCTTACAAAGAAATGTTTCATATGGTCAAATCCATGGCTTCTGGACTCCAAAAATTCGGTATATCACAAGGGGATGTAGTTTTGCTTGTGTTACCAAATTCCATTTTCTATCCCATTATTCTTTTAGGTGTTCTGTATTTAGGAGCAGTTGTTACAACCATGTTTCCTCAAAGCAGTTCTTCAGAAATCAAGAAACGAATCAATGATTGCAATGTTCGATTGGCTTTTACAATAACTCAGAAAATCAAGAATTTTGAGTCATTGGGAATTCAGGCAATTGGGGTACCAGAAATTACCAATTTCGACTTGATGCAGCCTATGGGATTTTCTTATTTTCATGAGCTTATTTCAAGTGGTTCTGATTTGAAACAAAGACCAGTGATTAGACAAGAAGATACAGCTGCTCTATTGTTTTCTTCAGGTACTACTGGAGTAAGCAAAGGGGTTATGTTAACACATAGAAATTTTATATCTACGATCGAGCTTTTTGTTCGGTTTGAAGCTTCACAGTACGAATATTTGCCTACAGAGAATGTGTATTTGGCTGCTATACCAATGTTTCATATATATGGTCTTTCCATTTTTGTGATGGGATTACTGTCATTGGGATCTAGTGTTGTTATAATGAGCAAATTTGATGTGAAAGAGGTAGTCAAGGCTATTGACAGATTTAAAGTCACACATTTTCCAGTTGTTCCACCTATAATGACAGCTATGGCTAGAACTGCAGAGAAAATTGGTGTGCATAGATTTAGAAGTTTGAAACAAGTTTCTTGTGGAGCTGCTTCTTTGAACAAGAAGACCATTGATGATTTTGTTAAGGCTTTCCCTCATGTTGACTTCATTCAGGGATATGGCATGACTGAGTCCACAGCTATAGGAACTCGCGGCTTCAATACCAAAAATGCTAGAAATTATACGTCAGTTGGACTTTTAGCTCCAAACACAGAAGCTAAAGTAGTTGATTGGGTTTCCGGATCTTTCATGCCTCCTGGTAAAACCGGCGAACTTTTGTTACGCGGACCCGGTTCAATGAAAGGGTACTTGAATAATCCAGAGGCAACAATGTCCACAATAGATAAAGAAAATTGGCTACATACTGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTATTGATCGTTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGATTGCTCCTACTGATTTAGAAGCTGTGGTGATGACACATCCAGAAGTTCTCGATACTGCGGTAACAGCGGCCAAAGATGAAGAATGTGGGGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGCAAACTCTCACAGAAAGATGTTATTGATTATGTTGCTCAACAGGTTGCACCATATAAAAAAGTAAGAAAAGTGGTTTTTACAGAATCAATACCAAAATCTGCTGCTGGCAAAGTACTTCGTAGAGAACTTGGGAAGCACTTGCCTTCTAAACTGTGA

Protein sequence

MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL

Homology

[Close]

BLAST of Bhi05G000002 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 644.8 bits (1662), Expect = 6.5e-185
Identity = 318/552 (57.61%), Postives = 429/552 (77.72%), Query Frame = 0


Query: 25  PRSEQTNKYPN-WYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGS 84
           P+++ +++ P  W+S  TGI  S   S  +P DP LD VS +FS KH+G++A+IDS TG 
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGF 71

Query: 85  SISYKEMFHMVKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQ 144
           SIS+ E+  MV+SMA+G+    G+ QGDVV LVLPNS+++P+I L ++ LGA+VTTM P 
Sbjct: 72  SISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPS 131

Query: 145 SSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELIS 204
           SS  EIKK++++C+V LAFT T+ ++   SLG+  I V E  +FD ++ +    F+ ++ 
Sbjct: 132 SSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMK 191

Query: 205 SGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTE 264
                  +P+I+Q+D AA+++SSGTTG SKGV+LTHRN I+++ELFVRFEASQYEY  + 
Sbjct: 192 ESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSS 251

Query: 265 NVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMT 324
           NVYLAA+P+ HIYGLS+FVMGLLSLGS++V+M +FD  +VV  I+RFK+THFPVVPP++ 
Sbjct: 252 NVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLM 311

Query: 325 AMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRG 384
           A+ + A+ +    F+SLKQVS GAA L++K I+DF++  PHVD IQGYGMTESTA+GTRG
Sbjct: 312 ALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRG 371

Query: 385 FNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMS 444
           FN++    Y+SVGLLAPN +AKVVDW SGSF+PPG  GEL ++GPG MKGYLNNP+AT  
Sbjct: 372 FNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQM 431

Query: 445 TIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVT 504
           +I +++WL TGDI YFD DGYL+++DR+KE+IKYKGFQIAP DLEAV+++HP ++D AVT
Sbjct: 432 SIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVT 491

Query: 505 AAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVL 564
           AA +EECGEIPVAFVV++  + LS++DVI YVA QVAPY+KVRKVV   SIPKS  GK+L
Sbjct: 492 AAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKIL 551

Query: 565 RRELGKHLPSKL 575
           R+EL + L + +
Sbjct: 552 RKELKRILTNSV 562

[Close]

BLAST of Bhi05G000002 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 449.1 bits (1154), Expect = 5.2e-126
Identity = 237/548 (43.25%), Postives = 358/548 (65.33%), Query Frame = 0


Query: 26  RSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSI 85
           RS   N    +YS  T I         +P +P LDV +FI S  H G  A ID+STG ++
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNL 66

Query: 86  SYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSS 145
           ++ E++  V+S+A  L + GI +G VVLL+ PNSI +P++ L V+ LGA++TT  P ++S
Sbjct: 67  TFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTS 126

Query: 146 SEIKKRINDCNVRLAFTITQKIKNF----ESLGIQAIGVPEITNFDLMQPMGFSYFHELI 205
           +EI K+I D N  LAFT +Q +       + L I  +    + +   ++ +      E  
Sbjct: 127 NEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE-- 186

Query: 206 SSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPT 265
            SG+ +K+R  + Q+DTA LL+SSGTTG+SKGV+ +HRN I+ ++  V    S       
Sbjct: 187 PSGNRVKER--VDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSD----DG 246

Query: 266 ENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIM 325
           E  ++  +PMFHIYGL+ F  GLL+ GS+++++SKF++ E++ AI +++ T  P+VPPI+
Sbjct: 247 EQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPIL 306

Query: 326 TAMARTAEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGT 385
            AM   A++I   +   S+  V CG A L+K+  + F + +P V  +QGYG+TEST IG 
Sbjct: 307 VAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGA 366

Query: 386 RGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEAT 445
                + +R Y + G L+ + E ++VD V+G  + P +TGEL L+GP  MKGY +N EAT
Sbjct: 367 STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEAT 426

Query: 446 MSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTA 505
            ST+D E WL TGD+ Y D DG+++V+DRLKE+IKYKG+Q+AP +LEA+++THPE+ D A
Sbjct: 427 SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAA 486

Query: 506 VTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGK 565
           V    D+E G+ P+A+VV+K GS LS+K ++++VA+QVAPYK++RKV F  SIPK+ +GK
Sbjct: 487 VIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGK 538

Query: 566 VLRRELGK 569
           +LR++L K
Sbjct: 547 ILRKDLIK 538

[Close]

BLAST of Bhi05G000002 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 420.6 bits (1080), Expect = 2.0e-117
Identity = 231/549 (42.08%), Postives = 341/549 (62.11%), Query Frame = 0


Query: 37  YSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKS 96
           +   T I  S      +P + FLDV SFI S  H G +  +D+ TG  +S+ E++  V+ 
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPELWLGVER 86

Query: 97  MASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCN 156
           +A  L   G+ +G+VV+++ PNSI +PI+ L V+ LGA++TT  P ++S EI K+I D  
Sbjct: 87  VAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGDSR 146

Query: 157 VRLAFTITQKIK--------NFESLGIQAIGVPEITNFDLMQPMG-FSYFHELISSGSDL 216
             LAFT  + +         N   + +    VP  +  D ++ +G      E   S S +
Sbjct: 147 PVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSESRV 206

Query: 217 KQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELF-VRFEASQYEYLPTENVYL 276
           KQR  + Q+DTAALL+SSGTTG SKGVML+HRN I+ ++ +  RF   Q          +
Sbjct: 207 KQR--VNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT--------I 266

Query: 277 AAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMAR 336
             IPM HI+G   F  GL++LG ++V++ KFD+ +++ A++  + ++  +VPPI+ AM  
Sbjct: 267 CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 326

Query: 337 TAEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNT 396
            A +I   +   SL  V  G A L+++  + FV+ +P V  +QGYG+TESTAI    FN 
Sbjct: 327 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 386

Query: 397 KNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTID 456
           +  + Y + GLLAPN E K+VD  +G  +   +TGEL +R P  MKGY  N EAT STID
Sbjct: 387 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 446

Query: 457 KENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAK 516
            E WL TGD+ Y D DG+++V+DRLKE+IK  G+Q+AP +LEA+++ HPE+ D AV    
Sbjct: 447 SEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIP 506

Query: 517 DEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRE 575
           D + G+ P+A++V+K GS LS+ +++ +VA+QV+PYKK+RKV F  SIPK+ +GK+LRRE
Sbjct: 507 DMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRE 565

[Close]

BLAST of Bhi05G000002 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 416.8 bits (1070), Expect = 2.9e-116
Identity = 223/540 (41.30%), Postives = 338/540 (62.59%), Query Frame = 0


Query: 42  GICRSVHASRGVPSDPFLDVVSFIF--SFKHNGNSAIIDSSTGSSISYKEMFHMVKSMAS 101
           GI RS+  +  +P DP   +VSF+F  S  +    AI DS TG S+++ ++   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 102 GLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRL 161
           G  + GI + DVVL+  PNS  +P+  L V  +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 162 AFTITQ---KIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPV--IR 221
             ++ Q   KIK F+ L +  +G  +              F  ++     + + P   I+
Sbjct: 130 IISVNQLFDKIKGFD-LPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIK 189

Query: 222 QEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHI 281
           Q DTAALL+SSGTTG SKGV LTH NFI+   +    +    EY     V+L  +PMFH+
Sbjct: 190 QSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFLPMFHV 249

Query: 282 YGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVH 341
           +GL++     L  G+++V M++F+++ V+K I++F+VTH  VVPP+  A+++ +  +   
Sbjct: 250 FGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQS-IVKKF 309

Query: 342 RFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSV 401
              SLK +  GAA L K  +++  +  P+V  +QGYGMTE+  I +        RN  S 
Sbjct: 310 DLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNSGSA 369

Query: 402 GLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGD 461
           G+LAP  EA++V   +G   PP + GE+ +RGP  MKGYLNNP+AT  TIDK++W+HTGD
Sbjct: 370 GMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGD 429

Query: 462 IVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPV 521
           + YF+ DG LYV+DR+KE+IKYKGFQ+AP +LE ++++HP++LD  V    DEE GE+P+
Sbjct: 430 LGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPI 489

Query: 522 AFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           AFVV+ P S ++++D+  ++A+QVAPYK++R+V F   +PKSAAGK+LRREL + + SK+
Sbjct: 490 AFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSKM 544

[Close]

BLAST of Bhi05G000002 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 2.7e-114
Identity = 225/548 (41.06%), Postives = 345/548 (62.96%), Query Frame = 0


Query: 39  PDTGICRS---VHASR---GVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISYKEMFH 98
           P +G C+S    ++ R    +P +   DV +FI S  H G +A ID++TG  +++ +++ 
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDLWR 71

Query: 99  MVKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKR 158
            V  +A  L  + GI +GDVVL++ PNSIF P++ L V+ LGAV TT    ++S EI K+
Sbjct: 72  AVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQ 131

Query: 159 INDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMG-FSYFHELIS---SGSDL 218
           I D N  L FT T+++     + I  +   +    +L   +       E++    SG  +
Sbjct: 132 IADSNPTLVFT-TRQLAPKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRV 191

Query: 219 KQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLA 278
           + R  + Q+DTA +L+SSGTTG SKGV+ +HRN  + +  F+       + L  +++++ 
Sbjct: 192 RDR--VNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKRDDIFIC 251

Query: 279 AIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMART 338
            +PMFH YGL  F MG ++LGS+VVI+ +F + +++ A+++ + T   + PP++ AM   
Sbjct: 252 TVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMIND 311

Query: 339 AEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTK 398
           A+ I   +   SLK V CG A L+K+  + F++ +P VD +QGY +TES   G    + +
Sbjct: 312 ADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAE 371

Query: 399 NARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDK 458
            +R Y + G L  + EA++VD  +G FM   +TGEL L+GP   KGY  N EAT  TI+ 
Sbjct: 372 ESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINL 431

Query: 459 ENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKD 518
           E WL TGD+ Y D DG+L+V+DRLKE+IKYKG+Q+ P +LEA+++THP++LD AV    D
Sbjct: 432 EGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPD 491

Query: 519 EECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRREL 575
           +E G+ P+A+VV+K  S LS+K VID++++QVAPYKK+RKV F  SIPK+A+GK LR++L
Sbjct: 492 KEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDL 550

[Close]

BLAST of Bhi05G000002 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 644.8 bits (1662), Expect = 9.1e-184
Identity = 318/552 (57.61%), Postives = 429/552 (77.72%), Query Frame = 0


Query: 25  PRSEQTNKYPN-WYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGS 84
           P+++ +++ P  W+S  TGI  S   S  +P DP LD VS +FS KH+G++A+IDS TG 
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGF 71

Query: 85  SISYKEMFHMVKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQ 144
           SIS+ E+  MV+SMA+G+    G+ QGDVV LVLPNS+++P+I L ++ LGA+VTTM P 
Sbjct: 72  SISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPS 131

Query: 145 SSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELIS 204
           SS  EIKK++++C+V LAFT T+ ++   SLG+  I V E  +FD ++ +    F+ ++ 
Sbjct: 132 SSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMK 191

Query: 205 SGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTE 264
                  +P+I+Q+D AA+++SSGTTG SKGV+LTHRN I+++ELFVRFEASQYEY  + 
Sbjct: 192 ESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSS 251

Query: 265 NVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMT 324
           NVYLAA+P+ HIYGLS+FVMGLLSLGS++V+M +FD  +VV  I+RFK+THFPVVPP++ 
Sbjct: 252 NVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLM 311

Query: 325 AMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRG 384
           A+ + A+ +    F+SLKQVS GAA L++K I+DF++  PHVD IQGYGMTESTA+GTRG
Sbjct: 312 ALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRG 371

Query: 385 FNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMS 444
           FN++    Y+SVGLLAPN +AKVVDW SGSF+PPG  GEL ++GPG MKGYLNNP+AT  
Sbjct: 372 FNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQM 431

Query: 445 TIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVT 504
           +I +++WL TGDI YFD DGYL+++DR+KE+IKYKGFQIAP DLEAV+++HP ++D AVT
Sbjct: 432 SIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVT 491

Query: 505 AAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVL 564
           AA +EECGEIPVAFVV++  + LS++DVI YVA QVAPY+KVRKVV   SIPKS  GK+L
Sbjct: 492 AAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKIL 551

Query: 565 RRELGKHLPSKL 575
           R+EL + L + +
Sbjct: 552 RKELKRILTNSV 562

[Close]

BLAST of Bhi05G000002 vs. ExPASy Swiss-Prot
Match: Q6YYZ2 (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 9.5e-149
Identity = 288/549 (52.46%), Postives = 372/549 (67.76%), Query Frame = 0


Query: 34  PNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFS---FKHNGNSAIIDSSTGSSISYKEM 93
           P +YS  TGI  S+H    +P+DP L +V+ +F+       G   ++D++T S++S  ++
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 94  FHMVKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIK 153
             +V S+A+GL ++ G+ +G VVLL+LPNS+ +P+  L VL  GAV TTM P SS +EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 154 KRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMG------FSYFHELISS 213
            +       L             L    + VPEI +       G      F+ F  ++  
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 214 GSDLKQR---PVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYL- 273
           G         PV+ Q+D  A+L+SSGT+G SKGV+LTHRN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 274 PTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPP 333
             ENVY+AA+PM H+YGLS+F +GLLS+G++VV+M +FD  + V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 334 IMTAMARTAEKIGV--HRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTA 393
           IM AM R A   GV   +  SL QVSCGAA +    I +F++AFPHVDFIQGYGMTESTA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 394 IGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNP 453
           +GTRGFNT   + YTSVGLLAPN  AK+V   S S +PPG +GEL L GPG MKGYL++ 
Sbjct: 385 VGTRGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDD 444

Query: 454 EATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVL 513
           +   +   K+ WL TGDI YFD DGYLY++ RLK+ IKYKGFQIAP DLE V++ HPE+L
Sbjct: 445 DDACTR--KDGWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEIL 504

Query: 514 DTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSA 567
           D AVT+A+DEE GEIPVAFVV++ GS LS K V++YVA+QVAPYK+VRKVVF E+IPKS 
Sbjct: 505 DVAVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKSP 564

[Close]

BLAST of Bhi05G000002 vs. ExPASy Swiss-Prot
Match: Q336M7 (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 472.2 bits (1214), Expect = 8.1e-132
Identity = 257/514 (50.00%), Postives = 344/514 (66.93%), Query Frame = 0


Query: 35  NWYSPDTGICRSVHASRGVPSDPFLDVVSFIFS-FKHNGNSAIIDSSTGSSISYKEMFHM 94
           ++YS  TG+  S+H    +PSDP L +V  +FS    + +S ++D+ T +++S  +   +
Sbjct: 14  SFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHHHSLLVDAPTAATLSCADFRRL 73

Query: 95  VKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRI 154
           V S+A+GL ++  I++G +VLL+LPNS+ +P+  L VL  GAV TTM P S+ +EI  R+
Sbjct: 74  VSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAARL 133

Query: 155 NDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELISSGSD--LKQR 214
            D    L    T        L    + VP+             +FH L+ +  +  ++  
Sbjct: 134 RDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEMG 193

Query: 215 PVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLAAIP 274
             + Q+D AA+L+SSGT+G SKGV++THRN I+ +ELFVRFEASQY     +NVYLAA+P
Sbjct: 194 VGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAALP 253

Query: 275 MFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEK 334
           MFH+YGLS+F +GLLSLG +VV+M +F+V + VKAI ++KVTH P+VPPIM+A+ R    
Sbjct: 254 MFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANPP 313

Query: 335 IGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKNARN 394
           +      SL QVS GAA LN   I  F+ AFPHVDFIQGYGMTESTA+GTRGFNT   + 
Sbjct: 314 L---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKHKK 373

Query: 395 YTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMK------GYLNNPEATMSTI 454
           Y SVGLLAPN  AK+V   SGS +PPG  GEL L GP  MK      GYLN+ +   +  
Sbjct: 374 YASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDDDAFTR- 433

Query: 455 DKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAA 514
            K+ WL TGDI YFD DGYL+++ RLK+ IKYKGFQIAP DLEAV++ HPE++D AVT+ 
Sbjct: 434 -KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVTSD 493

Query: 515 KDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQV 539
           +DEE GEIPVAFVV+K GS LS   V++YVA+QV
Sbjct: 494 EDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQV 522

[Close]

BLAST of Bhi05G000002 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 449.1 bits (1154), Expect = 7.4e-125
Identity = 237/548 (43.25%), Postives = 358/548 (65.33%), Query Frame = 0


Query: 26  RSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSI 85
           RS   N    +YS  T I         +P +P LDV +FI S  H G  A ID+STG ++
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNL 66

Query: 86  SYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSS 145
           ++ E++  V+S+A  L + GI +G VVLL+ PNSI +P++ L V+ LGA++TT  P ++S
Sbjct: 67  TFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTS 126

Query: 146 SEIKKRINDCNVRLAFTITQKIKNF----ESLGIQAIGVPEITNFDLMQPMGFSYFHELI 205
           +EI K+I D N  LAFT +Q +       + L I  +    + +   ++ +      E  
Sbjct: 127 NEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE-- 186

Query: 206 SSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPT 265
            SG+ +K+R  + Q+DTA LL+SSGTTG+SKGV+ +HRN I+ ++  V    S       
Sbjct: 187 PSGNRVKER--VDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSD----DG 246

Query: 266 ENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIM 325
           E  ++  +PMFHIYGL+ F  GLL+ GS+++++SKF++ E++ AI +++ T  P+VPPI+
Sbjct: 247 EQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPIL 306

Query: 326 TAMARTAEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGT 385
            AM   A++I   +   S+  V CG A L+K+  + F + +P V  +QGYG+TEST IG 
Sbjct: 307 VAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGA 366

Query: 386 RGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEAT 445
                + +R Y + G L+ + E ++VD V+G  + P +TGEL L+GP  MKGY +N EAT
Sbjct: 367 STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEAT 426

Query: 446 MSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTA 505
            ST+D E WL TGD+ Y D DG+++V+DRLKE+IKYKG+Q+AP +LEA+++THPE+ D A
Sbjct: 427 SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAA 486

Query: 506 VTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGK 565
           V    D+E G+ P+A+VV+K GS LS+K ++++VA+QVAPYK++RKV F  SIPK+ +GK
Sbjct: 487 VIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGK 538

Query: 566 VLRRELGK 569
           +LR++L K
Sbjct: 547 ILRKDLIK 538

[Close]

BLAST of Bhi05G000002 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 7.4e-125
Identity = 229/530 (43.21%), Postives = 352/530 (66.42%), Query Frame = 0


Query: 53  VPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVV 112
           +P +  +DV +FI S  H+G  A ID++TG  +++ +++  V S+A+ L   GI +GDV+
Sbjct: 31  LPQNHSVDVTTFISSRAHHGKIAFIDAATGRHLTFPQLWRAVDSVATCLSAMGIRKGDVI 90

Query: 113 LLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFES 172
           LL+ PNSI++P++ L V+ LGA++TT  P ++  EI K+I D    LAFTI Q +     
Sbjct: 91  LLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITDSKPVLAFTIPQLVSKIAG 150

Query: 173 LGIQAIGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPV-------IRQEDTAALLFSS 232
             +  + + +     L + +       + S G  +++ P        + QEDTA LL+SS
Sbjct: 151 SNLPIVIIDDEVKSSLEKTLNI-----VSSLGEMMRKEPSPNRIGYRVNQEDTATLLYSS 210

Query: 233 GTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLL 292
           GTTG SKGV+ +H+N I+ ++  +    S++     E+ ++  +PMFHIYGL+ F MGLL
Sbjct: 211 GTTGASKGVVSSHKNLIAMVQTIL----SRFGTEDGEHTFICTVPMFHIYGLAAFAMGLL 270

Query: 293 SLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKI-GVHRFRSLKQVSC 352
           S GS++VI+SKF++ E++ AI++++ T+ P+VPPI+ A+ + A  I   +   SL+ V  
Sbjct: 271 SSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIRAKYDLSSLQSVLS 330

Query: 353 GAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAK 412
           G A L+K+ I+ FV+ +P V  +QGYG+TEST IG      + +R Y + G+L+P+ EAK
Sbjct: 331 GGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESRRYGTAGMLSPSMEAK 390

Query: 413 VVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYL 472
           +V+  +G  +   +TGEL LRGP  MKGY +N EAT STID E WL TGD+ Y D DG++
Sbjct: 391 IVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGWLRTGDLCYIDEDGFI 450

Query: 473 YVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSK 532
           +V+DRLKE+IKYKG+Q+AP +LEA++++HPE+ D AV    D+E G+ P+A+VV+K GS 
Sbjct: 451 FVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKEAGQFPMAYVVRKGGSN 510

Query: 533 LSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           LS+  V+D++A+ VAPYK++RKV F  SIPK+ +GK+LR++L K   SKL
Sbjct: 511 LSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIKLATSKL 551

[Close]

BLAST of Bhi05G000002 vs. ExPASy TrEMBL
Match: A0A0A0L8X1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 4.1e-288
Identity = 499/574 (86.93%), Postives = 542/574 (94.43%), Query Frame = 0


Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MA L+N+ FS++TPENE VITKKIPRSE+TNKYPNWYSPDTGICRSVH  R +PSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFS--FKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPN 120
           VVSFIFS  F+HNG+SA+IDSSTG+SISYKE+F MVKSMASGL   GISQGDVVLL+LPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAI 180
           SIF+PII+LG LYLGAVVTTMFPQSSSSEIKKRI DCNVRLAF ITQKIKNFE+LGI+ I
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTH 240
           GVPE TNF+LM+ MGFS F+ELIS GSDL ++PVIRQEDTAA+LFSSGTTGVSKGVML+H
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFD 300
           RNFISTIELFVRFEASQYEYLPT+NVYLAA+PMFHIYGLSIFVMGL+SLGSSVV+MSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFV 360
           VK+VVKAIDRFKVTHFPVVPPI+TA+ARTAEKIGVHRFRSLKQVSCGAA+ +KKTID+FV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 KAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGK 420
            A PHVDFIQGYGMTESTA+GTRGFNTKNARNY SVGLLAPNTEAKVVDWV+GS MPPGK
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEAT  T+D+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQV 540
           FQIAPTDLEAVV+THPEVLDTAV AAKDEECGEIPVAFVVKKPGS L+QKDV+DYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVVFTESIPKSAAGKVLRRELGKHLPS 573
           APYKKVRKV+FTESIPKSAAGKVLRREL KH P+
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPA 574

[Close]

BLAST of Bhi05G000002 vs. ExPASy TrEMBL
Match: A0A5D3DC80 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00450 PE=4 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 1.3e-286
Identity = 502/575 (87.30%), Postives = 538/575 (93.57%), Query Frame = 0


Query: 1   MAPLL-NDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFL 60
           MA LL ND FS++TPENE  ITKKIP SE+TNKYPNWYSPDTGIC SVH  R +PSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFS--FKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLP 120
           DVVSFIFS  F+HNG+SA+IDS TG+SISYKE+F MVKSMASGL   GISQGDVVLL+LP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQA 180
           NSIFYPII+LG LYLGAV+TTMFPQSSSSEIKKRI DCNVRLAFTITQKIKNFE+LGI+ 
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLT 240
           IGVPE TNFDLM+PMGFS F+ELISSGSDL +RPVIRQEDTAA+LFSSGTTGVSKGVML+
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKF 300
           HRNFISTIELFVRFEASQYEYLPT+NVYLAAIPMFHIYGLSIFVMGL+SLGSSVV+MSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDF 360
           DVK+VVKAIDRFKVTHFPVVPPI+ A++RTAEKIGV RFRSLKQVSCGAA+ +KKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VKAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPG 420
           V+A  HVDFIQGYGMTESTAIGTRGFN K  RNY SVGLLAPNTEAKVVDWV+GS MPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQ 540
           GFQIAPTDLEAVV+ HPEVLDTAV AAKDEECGEIPVAFVVKKPGS LSQKDV+DYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVVFTESIPKSAAGKVLRRELGKHLPS 573
           VAPYKKVRKV+FTESIPKSAAGKVLRREL KHLP+
Sbjct: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPA 575

[Close]

BLAST of Bhi05G000002 vs. ExPASy TrEMBL
Match: A0A6J1HGK9 (4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464107 PE=4 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.3e-281
Identity = 486/574 (84.67%), Postives = 528/574 (91.99%), Query Frame = 0


Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MAPLLND FS+QTPE++TV TKKIP     NKYP WYSPDTGICRSVHASR +P+DPFLD
Sbjct: 1   MAPLLNDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 60

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           V S+I SF+HNG SA+IDSSTG SISY+E++ MV SMASGLQK G+SQGDVVLL+LPNSI
Sbjct: 61  VASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSI 120

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           FYPI+LLGVLYLGAV+TTMFPQSSS EIKKRI++CNVRLAF   Q + NFE+ G+QAIGV
Sbjct: 121 FYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAIGV 180

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE TN DLM+PMGFS F+ELIS G DL +RPVIRQEDTAA+LFSSGTTGVSKGV+LTHRN
Sbjct: 181 PENTNLDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRN 240

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FIST+ELFVRFEASQYEYL TENVYLAAIPMFH+YGLSIFVMGLLSLGSS+V+MSKFDVK
Sbjct: 241 FISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVK 300

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EVV AIDRFKVTHFPVVPPIM  MARTA K G H+FRSLKQVSCGAASL+KK I DFV+A
Sbjct: 301 EVVVAIDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQA 360

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            PHVDFIQGYGMTE+TA+GTRGFNTK A+NY SVGLLAPN EAKVVDWVSGS MPPGKTG
Sbjct: 361 LPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTG 420

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           ELLLRGPG MKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKGFQ
Sbjct: 421 ELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQ 480

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAPTDLE+VV+THPEVLDTAV AA+DEECGEIPVAFVVKKPGS LSQKDVIDYVAQQVAP
Sbjct: 481 IAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAP 540

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKK+RKVVFTESIPKSAAGKVLR+ELGKHLPSKL
Sbjct: 541 YKKIRKVVFTESIPKSAAGKVLRKELGKHLPSKL 569

[Close]

BLAST of Bhi05G000002 vs. ExPASy TrEMBL
Match: A0A6J1HHI8 (4-coumarate--CoA ligase-like 6 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464107 PE=4 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.3e-281
Identity = 486/574 (84.67%), Postives = 528/574 (91.99%), Query Frame = 0


Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MAPLLND FS+QTPE++TV TKKIP     NKYP WYSPDTGICRSVHASR +P+DPFLD
Sbjct: 5   MAPLLNDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 64

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           V S+I SF+HNG SA+IDSSTG SISY+E++ MV SMASGLQK G+SQGDVVLL+LPNSI
Sbjct: 65  VASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSI 124

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           FYPI+LLGVLYLGAV+TTMFPQSSS EIKKRI++CNVRLAF   Q + NFE+ G+QAIGV
Sbjct: 125 FYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAIGV 184

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE TN DLM+PMGFS F+ELIS G DL +RPVIRQEDTAA+LFSSGTTGVSKGV+LTHRN
Sbjct: 185 PENTNLDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRN 244

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FIST+ELFVRFEASQYEYL TENVYLAAIPMFH+YGLSIFVMGLLSLGSS+V+MSKFDVK
Sbjct: 245 FISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVK 304

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EVV AIDRFKVTHFPVVPPIM  MARTA K G H+FRSLKQVSCGAASL+KK I DFV+A
Sbjct: 305 EVVVAIDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQA 364

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            PHVDFIQGYGMTE+TA+GTRGFNTK A+NY SVGLLAPN EAKVVDWVSGS MPPGKTG
Sbjct: 365 LPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTG 424

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           ELLLRGPG MKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKGFQ
Sbjct: 425 ELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQ 484

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAPTDLE+VV+THPEVLDTAV AA+DEECGEIPVAFVVKKPGS LSQKDVIDYVAQQVAP
Sbjct: 485 IAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAP 544

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKK+RKVVFTESIPKSAAGKVLR+ELGKHLPSKL
Sbjct: 545 YKKIRKVVFTESIPKSAAGKVLRKELGKHLPSKL 573

[Close]

BLAST of Bhi05G000002 vs. ExPASy TrEMBL
Match: A0A6J1HUG7 (4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466820 PE=4 SV=1)

HSP 1 Score: 963.4 bits (2489), Expect = 4.3e-277
Identity = 481/574 (83.80%), Postives = 522/574 (90.94%), Query Frame = 0


Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MAPLLND FS+QTPE++ V TKKIP     NKYP WYSPDTGICRSVHASR +P+DPFLD
Sbjct: 1   MAPLLNDQFSFQTPESQAVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 60

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           V S+I SF+HNG SA+IDSSTG SISY+E++ MV SMASGLQK G+SQGDVVLL+LPNSI
Sbjct: 61  VASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSI 120

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           FYPI+LLGVLYLGAV+TTMFPQSSS EIKKRI++CNVRLAF   Q + NFE+LG+QAIGV
Sbjct: 121 FYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGV 180

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE TN DLM+PMGFS F+ELISSG DL +R VIRQEDTAA+LFSSGTTGVSKGV+LTHRN
Sbjct: 181 PENTNLDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRN 240

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FISTIELFVRFEASQYEYL TENVYLAAIPMFH+YGLSIFVMGLLSLGSS+V+MSKFDVK
Sbjct: 241 FISTIELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVK 300

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EVV AIDRFKVTHFPVVPPIM  MAR A K G HRFRSLKQVSCGAASL+ K + DFV+A
Sbjct: 301 EVVMAIDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQA 360

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            PHVDFIQGYGMTE+TA+GTRGFNTK A+N+ SVGLLAPN EAKVVDWVS S MPPGKTG
Sbjct: 361 LPHVDFIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTG 420

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           ELLLRGPG MKGYLNNPEATMSTID+ENWLHTGDI YFDRDGYLYV+DRLKEVIKYKGFQ
Sbjct: 421 ELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ 480

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAPTDLE+VV+THPEVLDTAV AA DEECGEIPVAFVVKKPGS LSQKDVIDYVAQQVAP
Sbjct: 481 IAPTDLESVVITHPEVLDTAVAAAVDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAP 540

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKK+RKVVFTESIPKSAAGKVLR+EL KHLPSKL
Sbjct: 541 YKKIRKVVFTESIPKSAAGKVLRKELVKHLPSKL 569

The following BLAST results are available for this feature:

BLAST of Bhi05G000002 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19010.1	6.5e-185	57.61	AMP-dependent synthetase and ligase family protein	[more]
AT1G20510.1	5.2e-126	43.25	OPC-8:0 CoA ligase1	[more]
AT1G20480.1	2.0e-117	42.08	AMP-dependent synthetase and ligase family protein	[more]
AT4G05160.1	2.9e-116	41.30	AMP-dependent synthetase and ligase family protein	[more]
AT1G20500.1	2.7e-114	41.06	AMP-dependent synthetase and ligase family protein	[more]

BLAST of Bhi05G000002 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84P24	9.1e-184	57.61	4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=...	[more]
Q6YYZ2	9.5e-149	52.46	4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3...	[more]
Q336M7	8.1e-132	50.00	4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2...	[more]
Q84P21	7.4e-125	43.25	4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=...	[more]
M4IQR7	7.4e-125	43.21	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]

BLAST of Bhi05G000002 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8X1	4.1e-288	86.93	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1	[more]
A0A5D3DC80	1.3e-286	87.30	4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1HGK9	1.3e-281	84.67	4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1HHI8	1.3e-281	84.67	4-coumarate--CoA ligase-like 6 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1HUG7	4.3e-277	83.80	4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.300.30		coord: 472..571 e-value: 1.1E-29 score: 105.1
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 28..572
None	No IPR available	PANTHER	PTHR24096:SF261	4-COUMARATE--COA LIGASE-LIKE 6	coord: 28..572
None	No IPR available	CDD	cd05904	4CL	coord: 53..566 e-value: 0.0 score: 743.67
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 69..570
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 56..471 e-value: 4.2E-117 score: 393.4
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 70..476 e-value: 3.9E-102 score: 342.0
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 486..560 e-value: 1.3E-16 score: 61.4
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 221..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000002	Bhi05M000002	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006744	ubiquinone biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0016405	CoA-ligase activity