Bhi04G001654 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G001654
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	nigrin b-like
Location	chr4: 55650799 .. 55651916 (-)
RNA-Seq Expression	Bhi04G001654
Synteny	Bhi04G001654

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAATAATACTATGTCCACAGCAGACGTTGGATATGTTATTCACTGCTGATAGTTTTACAATACCATGGCCATTGCTATTACTATAAAAACATAGTGCAAGCTCAGGGATGGATCACACAATCACAAGATAGACGATGGAGATCAAGAAGATGACAATAGTTTTTCTTGTTGTAGTTTTAGCTGCTTCATTCACGGGGAACCACGATGTCTCAGCAGCTCGCACGATTGTCCTCAGTAGATCGACACATCTTGTTGGCCGAGACGGGCTATGTCTCGACGTGATCGGAGGGTACAGCGACAGCCATATTCCAACTCAACTATGGCCATGTGGACCTCAAAACAACCAGCTATGGACAATTCAAGTTGATGGAACAATTCGAACGATGGGAAAATGCTTAGTTCCCAATGGCCACGAACCTGGATCCTACACCATGATTGATGACTGCAACAAGGTTGATACACAGGACAAGACATGGAAATTATATCCCGATGGCACCCTCGTTCACGTATGGTCCGGCCTCGTCTTGACGGGTCAAGGAACGGGACCACACGCCATAACCACAATAGAAACTAACACATCCGCCCCAACACAAAGCTGGGGAACGGCGGACGATGGATCGCCCATTGTCGTCGCCATTGTAGGGCTTCGTCACATGTGCTTACAAGCGCAGAATGACAACGTCCATGTCTGGATGAATAGCTGCGTCAAAAACAACAGACAACAATATTGGGCAGTTTATAATGACGGTACAATTCGAACCAACTACAACACAAGCTTTTGCTTGAGTTCCACAGGCAAAGGTTTAAATGACGCTATTGGTTTAGCTAAATGTCAAGGGTTGCCTCAACAGCGGTGGGTGGCTAAGGAGGATGGCACCATCTTAAACCCCATCAATAATATGGTGATGGATGTGAAAGGGAGTGATCCTAATCTTCGCAAAATTATTCTCTTTGCTCCTACGGGTAACCCTAATCAGCAGTGGGTGTTCGTTACTCAATATAAACCCTAAATATGTGTAATGCAATGCATTGCATGCTTTAAATAATACTTGTATTGTATGGCGTAGTGTTGCTTACAAATAAAGAATAAATAAATGTGTGGTTTTTTTAATAACG

mRNA sequence

AATAATAATACTATGTCCACAGCAGACGTTGGATATGTTATTCACTGCTGATAGTTTTACAATACCATGGCCATTGCTATTACTATAAAAACATAGTGCAAGCTCAGGGATGGATCACACAATCACAAGATAGACGATGGAGATCAAGAAGATGACAATAGTTTTTCTTGTTGTAGTTTTAGCTGCTTCATTCACGGGGAACCACGATGTCTCAGCAGCTCGCACGATTGTCCTCAGTAGATCGACACATCTTGTTGGCCGAGACGGGCTATGTCTCGACGTGATCGGAGGGTACAGCGACAGCCATATTCCAACTCAACTATGGCCATGTGGACCTCAAAACAACCAGCTATGGACAATTCAAGTTGATGGAACAATTCGAACGATGGGAAAATGCTTAGTTCCCAATGGCCACGAACCTGGATCCTACACCATGATTGATGACTGCAACAAGGTTGATACACAGGACAAGACATGGAAATTATATCCCGATGGCACCCTCGTTCACGTATGGTCCGGCCTCGTCTTGACGGGTCAAGGAACGGGACCACACGCCATAACCACAATAGAAACTAACACATCCGCCCCAACACAAAGCTGGGGAACGGCGGACGATGGATCGCCCATTGTCGTCGCCATTGTAGGGCTTCGTCACATGTGCTTACAAGCGCAGAATGACAACGTCCATGTCTGGATGAATAGCTGCGTCAAAAACAACAGACAACAATATTGGGCAGTTTATAATGACGGTACAATTCGAACCAACTACAACACAAGCTTTTGCTTGAGTTCCACAGGCAAAGGTTTAAATGACGCTATTGGTTTAGCTAAATGTCAAGGGTTGCCTCAACAGCGGTGGGTGGCTAAGGAGGATGGCACCATCTTAAACCCCATCAATAATATGGTGATGGATGTGAAAGGGAGTGATCCTAATCTTCGCAAAATTATTCTCTTTGCTCCTACGGGTAACCCTAATCAGCAGTGGGTGTTCGTTACTCAATATAAACCCTAAATATGTGTAATGCAATGCATTGCATGCTTTAAATAATACTTGTATTGTATGGCGTAGTGTTGCTTACAAATAAAGAATAAATAAATGTGTGGTTTTTTTAATAACG

Coding sequence (CDS)

ATGGAGATCAAGAAGATGACAATAGTTTTTCTTGTTGTAGTTTTAGCTGCTTCATTCACGGGGAACCACGATGTCTCAGCAGCTCGCACGATTGTCCTCAGTAGATCGACACATCTTGTTGGCCGAGACGGGCTATGTCTCGACGTGATCGGAGGGTACAGCGACAGCCATATTCCAACTCAACTATGGCCATGTGGACCTCAAAACAACCAGCTATGGACAATTCAAGTTGATGGAACAATTCGAACGATGGGAAAATGCTTAGTTCCCAATGGCCACGAACCTGGATCCTACACCATGATTGATGACTGCAACAAGGTTGATACACAGGACAAGACATGGAAATTATATCCCGATGGCACCCTCGTTCACGTATGGTCCGGCCTCGTCTTGACGGGTCAAGGAACGGGACCACACGCCATAACCACAATAGAAACTAACACATCCGCCCCAACACAAAGCTGGGGAACGGCGGACGATGGATCGCCCATTGTCGTCGCCATTGTAGGGCTTCGTCACATGTGCTTACAAGCGCAGAATGACAACGTCCATGTCTGGATGAATAGCTGCGTCAAAAACAACAGACAACAATATTGGGCAGTTTATAATGACGGTACAATTCGAACCAACTACAACACAAGCTTTTGCTTGAGTTCCACAGGCAAAGGTTTAAATGACGCTATTGGTTTAGCTAAATGTCAAGGGTTGCCTCAACAGCGGTGGGTGGCTAAGGAGGATGGCACCATCTTAAACCCCATCAATAATATGGTGATGGATGTGAAAGGGAGTGATCCTAATCTTCGCAAAATTATTCTCTTTGCTCCTACGGGTAACCCTAATCAGCAGTGGGTGTTCGTTACTCAATATAAACCCTAA

Protein sequence

MEIKKMTIVFLVVVLAASFTGNHDVSAARTIVLSRSTHLVGRDGLCLDVIGGYSDSHIPTQLWPCGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGSYTMIDDCNKVDTQDKTWKLYPDGTLVHVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRHMCLQAQNDNVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCLSSTGKGLNDAIGLAKCQGLPQQRWVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPTGNPNQQWVFVTQYKP

Homology

[Close]

BLAST of Bhi04G001654 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 6.3e-64
Identity = 118/262 (45.04%), Postives = 156/262 (59.54%), Query Frame = 0


Query: 30  TIVLSRSTHLVGRDGLCLDVIGGYSDSHIPTQLWPCGPQNNQLWTIQVDGTIRTMGKCLV 89
           T+  S + ++VGRDGLC+DV  GY     P QLWPCG Q NQ WT   D TIR+MGKC+ 
Sbjct: 303 TLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRSMGKCMT 362

Query: 90  PNGHEPGSYTMIDDCNKVDTQDKTWKLYPDGTLVHVWSGLVLTGQGTGPHAITTIETNTS 149
            NG   GS  +I +C+        W++  DG++++  SGLV+T        I  +E N  
Sbjct: 363 ANGLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIY 422

Query: 150 APTQSWGTADDGSPIVVAIVGLRHMCLQAQNDNVHVWMNSCVKNNRQQYWAVYNDGTIRT 209
           A +Q W   ++  PIV +IVG + MCLQ+  +N  VWM  C   + QQ WA+Y D TIR 
Sbjct: 423 AASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRTIRV 482

Query: 210 NYNTSFCLSSTGKGLNDAIGLAKCQGLPQQRWVAKEDGTILNPINNMVMDVKGSDPNLRK 269
           N     C+++ G    D I + KCQGLP QRW    DG I+NP +  VMDV+ S+ +LR+
Sbjct: 483 NSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVSLRE 542

Query: 270 IILFAPTGNPNQQWVFVTQYKP 292
           II+F  TGNPNQQW  VTQ  P
Sbjct: 543 IIIFPATGNPNQQW--VTQVLP 562

[Close]

BLAST of Bhi04G001654 vs. ExPASy Swiss-Prot
Match: Q9M653 (Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.2e-62
Identity = 118/254 (46.46%), Postives = 155/254 (61.02%), Query Frame = 0


Query: 41  GRDGLCLDVIGGYSDSHIPTQLWPCGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGSYTM 100
           G DGLC+DV    ++  IP QLWPCG Q NQ WT   DGTI++MGKC+  NG+ PG Y M
Sbjct: 346 GLDGLCMDVRNESNNDGIPIQLWPCGAQRNQQWTFHTDGTIQSMGKCMTSNGYHPGDYVM 405

Query: 101 IDDCNKVDTQDKT-WKLYPDGTLVHVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGTAD 160
           I +C+     D T W +  DG++ +  SGLVLT        I  +  NT +  Q     D
Sbjct: 406 IFNCSTAPVPDATKWVVSIDGSITNPHSGLVLTAPQAAQTTILLVVRNTHSAKQGRSVGD 465

Query: 161 DGSPIVVAIVGLRHMCLQAQND-NVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCLS 220
           D  PIV  IVG ++MCLQ  N+ N  VW+  C  +  QQ+WA+Y+DGTIR + + S C++
Sbjct: 466 DVEPIVTYIVGFKYMCLQGNNENNTRVWLEDCAVDRPQQWWALYSDGTIRVDSDRSLCVT 525

Query: 221 STGKGLNDAIGLAKCQGLPQQRWVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPTGN 280
           S G    DAI +  C G   QR V   DGTILNP   +VMDV+ S+  LR+IIL+ PTGN
Sbjct: 526 SDGHSSRDAIIILTCDGGINQRLVFNTDGTILNPNAQLVMDVRQSNVALRQIILYQPTGN 585

Query: 281 PNQQWV-FVTQYKP 292
           PNQQW+  +T+ +P
Sbjct: 586 PNQQWMTMITRTRP 599

[Close]

BLAST of Bhi04G001654 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.7e-58
Identity = 116/256 (45.31%), Postives = 148/256 (57.81%), Query Frame = 0


Query: 32  VLSRSTHLVGRDGLCLDVIGGYSDSHIPTQLWPCGPQNNQLWTIQVDGTIRTMGKCLVPN 91
           V+  +  + G DGLC+DV  G+     P QL PCG + NQLWT + DGTIR +GKCL  +
Sbjct: 318 VVEVTRRISGWDGLCVDVRYGHYIDGNPVQLRPCGNECNQLWTFRTDGTIRWLGKCLTAS 377

Query: 92  GHEPGSYTMIDDCNKVDTQDKTWKLYPDGTLVHVWSGLVLTGQGTGPHAITTIETNTSAP 151
                S  MI DCN V  +   W +  DGT+ +  SGLVLT          ++E N  A 
Sbjct: 378 -----SSVMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAA 437

Query: 152 TQSWGTADDGSPIVVAIVGLRHMCLQAQNDNVHVWMNSCVKNNRQQYWAVYNDGTIRTNY 211
            Q W T  D  P+V  IVG + MCL+   +N  VW+  CV N  QQ WA+Y DGTIR N 
Sbjct: 438 RQGW-TVGDVEPLVTFIVGYKQMCLRENGENNFVWLEDCVLNRVQQEWALYGDGTIRVNS 497

Query: 212 NTSFCLSSTGKGLNDAIGLAKCQGLPQQRWVAKEDGTILNPINNMVMDVKGSDPNLRKII 271
           N S C++S     +D I + KC+G   QRWV   +GTI NP   ++MDV   D +LRKII
Sbjct: 498 NRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLLMDVAQRDVSLRKII 557

Query: 272 LFAPTGNPNQQWVFVT 288
           L+ PTGNPNQQW+  T
Sbjct: 558 LYRPTGNPNQQWITTT 567

[Close]

BLAST of Bhi04G001654 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 8.8e-58
Identity = 116/257 (45.14%), Postives = 148/257 (57.59%), Query Frame = 0


Query: 32  VLSRSTHLVGRDGLCLDVIGGYSDSHIPTQLWPCGPQNNQLWTIQVDGTIRTMGKCLVPN 91
           V+  +  + G DGLC+DV  G+       QL PCG + NQLWT + DGTIR +GKCL  +
Sbjct: 318 VVEVTRRISGWDGLCVDVRDGHYIDGNTVQLGPCGNECNQLWTFRTDGTIRWLGKCLTTS 377

Query: 92  GHEPGSYTMIDDCNKVDTQDKTWKLYPDGTLVHVWSGLVLTGQGTGPHAITTIETNTSAP 151
                S  MI DCN V  +   W +  DGT+ +  SGLVLT          ++E N  A 
Sbjct: 378 -----SSVMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSLENNIHAA 437

Query: 152 TQSWGTADDGSPIVVAIVGLRHMCLQAQNDNVHVWMNSCVKNNRQQYWAVYNDGTIRTNY 211
            Q W T  D  P+V  IVG + MCL    +N  VW+  CV N  +Q WA+Y DGTIR N 
Sbjct: 438 RQGW-TVGDVEPLVTFIVGYKQMCLTENGENNFVWLEDCVLNRVEQEWALYGDGTIRVNS 497

Query: 212 NTSFCLSSTGKGLNDAIGLAKCQGLPQQRWVAKEDGTILNPINNMVMDVKGSDPNLRKII 271
           N S C++S     +D I + KC+G   QRWV   +GTI NP   +VMDV  S+ +LRKII
Sbjct: 498 NRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLVMDVAQSNVSLRKII 557

Query: 272 LFAPTGNPNQQWVFVTQ 289
           L+ PTGNPNQQW+  TQ
Sbjct: 558 LYPPTGNPNQQWITTTQ 568

[Close]

BLAST of Bhi04G001654 vs. ExPASy Swiss-Prot
Match: P11140 (Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 5.0e-53
Identity = 106/248 (42.74%), Postives = 150/248 (60.48%), Query Frame = 0


Query: 41  GRDGLCLDVI-GGYSDSHIPTQLWPCGP--QNNQLWTIQVDGTIRTMGKCLVPNGHEPGS 100
           GRDG+C+DV   GY + +    +W C    + NQLWT++ D TIR+ GKCL   G+ PGS
Sbjct: 281 GRDGMCVDVYDNGYHNGN-RIIMWKCKDRLEENQLWTLKSDKTIRSNGKCLTTYGYAPGS 340

Query: 101 YTMIDDCNKVDTQDKTWKLYPDGTLVHVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGT 160
           Y MI DC     +   W+++ +GT+++  S LVL+ + +      T++TN     Q W T
Sbjct: 341 YVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQGWRT 400

Query: 161 ADDGSPIVVAIVGLRHMCLQAQNDNVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCL 220
            ++ SP V +I G   +C+QAQ  N  VWM  C  N ++Q WA+Y DG+IR+  NT+ CL
Sbjct: 401 GNNTSPFVTSISGYSDLCMQAQGSN--VWMADCDSNKKEQQWALYTDGSIRSVQNTNNCL 460

Query: 221 SSTGKGLNDAIGLAKC-QGLPQQRWVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPT 280
           +S        I L  C  G   QRWV K DG+I +  ++MVMDVKGSDP+L++IIL+  T
Sbjct: 461 TSKDHKQGSTILLMGCSNGWASQRWVFKNDGSIYSLYDDMVMDVKGSDPSLKQIILWPYT 520

Query: 281 GNPNQQWV 285
           G PNQ W+
Sbjct: 521 GKPNQIWL 525

[Close]

BLAST of Bhi04G001654 vs. ExPASy TrEMBL
Match: A0A5A7UV90 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00550 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.7e-150
Identity = 253/291 (86.94%), Postives = 268/291 (92.10%), Query Frame = 0


Query: 1   MEIKKMTIVFLVVVLAASFTGNHDVSAARTIVLSRSTHLVGRDGLCLDVIGGYSDSHIPT 60
           MEIK  TI+F+ VVLAAS TG H+VSAAR IVLSRSTHLVG+DGLCLDVIGGYSD+H+PT
Sbjct: 6   MEIKSTTIIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPT 65

Query: 61  QLWPCGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGSYTMIDDCNKVDTQDKTWKLYPDG 120
           QLWPCGPQNNQLWTIQ DGTIRTMGKCLVPNGH+PGSYTMIDDCNK D  DKTWKLYPDG
Sbjct: 66  QLWPCGPQNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDG 125

Query: 121 TLVHVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRHMCLQAQN 180
           TL HV S LVLT QGTG +AITTIETNTSAPTQSWGTADDGSPIVVAIVGLR MCLQAQN
Sbjct: 126 TLTHVRSSLVLTSQGTGAYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQN 185

Query: 181 DNVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCLSSTGKGLNDAIGLAKCQGLPQQR 240
           DN+HVWMNSCVKNNRQQYWAV++DGTIR NYNTSFCLSSTGKG NDAI LAKC+GLPQQR
Sbjct: 186 DNIHVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQR 245

Query: 241 WVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPTGNPNQQWVFVTQYKP 292
           WVAKEDGTIL+   N+VMDVKGSDPNLRKIILFAPTGNPNQ W+FVTQYKP
Sbjct: 246 WVAKEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMFVTQYKP 296

[Close]

BLAST of Bhi04G001654 vs. ExPASy TrEMBL
Match: A0A1S4E5V9 (nigrin b-like OS=Cucumis melo OX=3656 GN=LOC107992255 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.7e-150
Identity = 253/291 (86.94%), Postives = 268/291 (92.10%), Query Frame = 0


Query: 1   MEIKKMTIVFLVVVLAASFTGNHDVSAARTIVLSRSTHLVGRDGLCLDVIGGYSDSHIPT 60
           MEIK  TI+F+ VVLAAS TG H+VSAAR IVLSRSTHLVG+DGLCLDVIGGYSD+H+PT
Sbjct: 1   MEIKSTTIIFVCVVLAASLTGIHNVSAARAIVLSRSTHLVGQDGLCLDVIGGYSDNHVPT 60

Query: 61  QLWPCGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGSYTMIDDCNKVDTQDKTWKLYPDG 120
           QLWPCGPQNNQLWTIQ DGTIRTMGKCLVPNGH+PGSYTMIDDCNK D  DKTWKLYPDG
Sbjct: 61  QLWPCGPQNNQLWTIQADGTIRTMGKCLVPNGHDPGSYTMIDDCNKADPNDKTWKLYPDG 120

Query: 121 TLVHVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRHMCLQAQN 180
           TL HV S LVLT QGTG +AITTIETNTSAPTQSWGTADDGSPIVVAIVGLR MCLQAQN
Sbjct: 121 TLTHVRSSLVLTSQGTGAYAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRRMCLQAQN 180

Query: 181 DNVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCLSSTGKGLNDAIGLAKCQGLPQQR 240
           DN+HVWMNSCVKNNRQQYWAV++DGTIR NYNTSFCLSSTGKG NDAI LAKC+GLPQQR
Sbjct: 181 DNIHVWMNSCVKNNRQQYWAVFSDGTIRANYNTSFCLSSTGKGSNDAIVLAKCEGLPQQR 240

Query: 241 WVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPTGNPNQQWVFVTQYKP 292
           WVAKEDGTIL+   N+VMDVKGSDPNLRKIILFAPTGNPNQ W+FVTQYKP
Sbjct: 241 WVAKEDGTILSLSTNLVMDVKGSDPNLRKIILFAPTGNPNQHWMFVTQYKP 291

[Close]

BLAST of Bhi04G001654 vs. ExPASy TrEMBL
Match: A0A6J1DVT2 (nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111023562 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 6.4e-128
Identity = 222/285 (77.89%), Postives = 240/285 (84.21%), Query Frame = 0


Query: 1   MEIKKMTIVFLVVVLAASFTGNHDVSAARTIVLSRSTHLVGRDGLCLDVIGGYSDSHIPT 60
           ME K MTIV L +VLAAS  G H VSAA TIV SRSTH+VGRDGLCLDVIGGY+D+HIPT
Sbjct: 1   MEKKMMTIV-LCLVLAASLVGIHHVSAAHTIVFSRSTHIVGRDGLCLDVIGGYNDNHIPT 60

Query: 61  QLWPCGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGSYTMIDDCNKVDTQDKTWKLYPDG 120
           QLW C PQN+QLWTIQ DGTIR+MGKCLVPNG  PGSYTMIDDCN  +  DKTWKLYPDG
Sbjct: 61  QLWTCNPQNHQLWTIQTDGTIRSMGKCLVPNGRSPGSYTMIDDCNDANPDDKTWKLYPDG 120

Query: 121 TLVHVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGTADDGSPIVVAIVGLRHMCLQAQN 180
           TL HV SGLVLT Q TGPH ITTIETNT A  QSW T DDG+PIV  IVGLR MCLQAQN
Sbjct: 121 TLAHVRSGLVLTAQATGPHTITTIETNTYAAMQSWTTGDDGTPIVTTIVGLRRMCLQAQN 180

Query: 181 DNVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCLSSTGKGLNDAIGLAKCQGLPQQR 240
           DNVHV MNSC+KNNRQQYWA+Y DGTIR N+NTS C+S TGKG NDAI LAKC+G PQQR
Sbjct: 181 DNVHVSMNSCMKNNRQQYWALYGDGTIRANFNTSLCMSGTGKGSNDAIVLAKCEGSPQQR 240

Query: 241 WVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPTGNPNQQWVF 286
           W+AKEDGTILN   N+VMDVKGSDPNLR+IILF  TGNPNQQW++
Sbjct: 241 WLAKEDGTILNLNTNLVMDVKGSDPNLRQIILFGRTGNPNQQWIW 284

[Close]

BLAST of Bhi04G001654 vs. ExPASy TrEMBL
Match: A0A6A4L4D0 (Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_15736 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 8.2e-67
Identity = 126/269 (46.84%), Postives = 165/269 (61.34%), Query Frame = 0


Query: 18  SFTGNHDVSAARTIVLSRSTHLVGRDGLCLDVIGGYSDSHIPTQLWPCG-PQNNQLWTIQ 77
           S  G+HD       +L+ + HL GRDG C+DV  G  +   P QLWPCG  Q+NQ WT +
Sbjct: 79  STVGDHD----ECTILNSTRHLSGRDGFCMDVRDGRDNDGNPIQLWPCGQQQSNQQWTFR 138

Query: 78  VDGTIRTMGKCLVPNGHEPGSYTMIDDCNKVDTQDKTWKLYPDGTLVHVWSGLVLTGQGT 137
            DGTIR++GKC+  +G+ PG Y MI DC         W L  DGT+ +  SGLVLT    
Sbjct: 139 TDGTIRSLGKCMTASGYSPGHYVMIFDCETAGQDATKWSLSTDGTITNPHSGLVLTATNE 198

Query: 138 GPHAIT-TIETNTSAPTQSWGTADDGSPIVVAIVGLRHMCLQAQNDNVHVWMNSCVKNNR 197
                T T+ETN  A +Q W   ++  P V  I+G   +C+QA NDN  VW+ SCV + +
Sbjct: 199 HVQGNTLTVETNIHAASQGWRVVEEVYPTVTTIMGFNDLCMQANNDNTRVWLESCVDSRQ 258

Query: 198 QQYWAVYNDGTIRTNYNTSFCLSSTGKGLNDAIGLAKCQGLPQQRWVAKEDGTILNPINN 257
           QQ WA+Y DGTIR N + + CL+S G    D I + +CQG   QRWV   DGTILNP   
Sbjct: 259 QQQWALYGDGTIRVNSDHNLCLTSDGHSSLDVIIVRRCQGWGNQRWVFHTDGTILNPNAK 318

Query: 258 MVMDVKGSDPNLRKIILFAPTGNPNQQWV 285
           +VMDV+ SD +L++IIL+ PTGNPNQ+W+
Sbjct: 319 LVMDVRQSDVSLKQIILYQPTGNPNQRWL 343

[Close]

BLAST of Bhi04G001654 vs. ExPASy TrEMBL
Match: Q945S4 (Lectin OS=Sambucus nigra OX=4202 GN=AIVl1 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 4.2e-63
Identity = 117/254 (46.06%), Postives = 155/254 (61.02%), Query Frame = 0


Query: 38  HLVGRDGLCLDVIGGYSDSHIPTQLWPCGPQNNQLWTIQVDGTIRTMGKCLVPNGHEPGS 97
           ++VGRDGLC+DV  GY     P QLWPCG Q NQ WT   D TIR+MGKC+  NG   GS
Sbjct: 64  NIVGRDGLCVDVRNGYDTDGTPIQLWPCGTQRNQQWTFHTDDTIRSMGKCMTANGLNNGS 123

Query: 98  YTMIDDCNKVDTQDKTWKLYPDGTLVHVWSGLVLTGQGTGPHAITTIETNTSAPTQSWGT 157
             MI +C+        W++  DG++++ +SGLV+T        I  ++ N  A +Q W  
Sbjct: 124 NIMIFNCSTAVENAIKWEVTIDGSIINPFSGLVITAPSAASRTILLLQNNIYAASQGWTV 183

Query: 158 ADDGSPIVVAIVGLRHMCLQAQNDNVHVWMNSCVKNNRQQYWAVYNDGTIRTNYNTSFCL 217
           +++  PIV +IVG R MCLQA  +N  VWM  C   + QQ WA++ D TIR N N   C+
Sbjct: 184 SNNVQPIVASIVGFREMCLQANGENNGVWMEDCEATSLQQQWALFGDRTIRVNSNRGLCV 243

Query: 218 SSTGKGLNDAIGLAKCQGLPQQRWVAKEDGTILNPINNMVMDVKGSDPNLRKIILFAPTG 277
           ++ G    D I + KCQGLP QRW    DG I+NP + +VMDVK S+ +LR+II++  TG
Sbjct: 244 TTNGYNSRDLIIILKCQGLPSQRWFFNSDGAIVNPKSKLVMDVKSSNVSLREIIIYPATG 303

Query: 278 NPNQQWVFVTQYKP 292
            PNQQW  VTQ  P
Sbjct: 304 RPNQQW--VTQVLP 315

The following BLAST results are available for this feature:

BLAST of Bhi04G001654 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi04G001654 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33183	6.3e-64	45.04	Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2	[more]
Q9M653	1.2e-62	46.46	Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt...	[more]
Q41358	6.7e-58	45.31	Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1	[more]
O22415	8.8e-58	45.14	Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=...	[more]
P11140	5.0e-53	42.74	Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2	[more]

BLAST of Bhi04G001654 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UV90	2.7e-150	86.94	Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00550 ...	[more]
A0A1S4E5V9	2.7e-150	86.94	nigrin b-like OS=Cucumis melo OX=3656 GN=LOC107992255 PE=3 SV=1	[more]
A0A6J1DVT2	6.4e-128	77.89	nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111023562 PE=3 SV=1	[more]
A0A6A4L4D0	8.2e-67	46.84	Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3...	[more]
Q945S4	4.2e-63	46.06	Lectin OS=Sambucus nigra OX=4202 GN=AIVl1 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 162..286 e-value: 3.0E-26 score: 103.2 coord: 29..158 e-value: 1.1E-13 score: 61.5
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 37..155 e-value: 1.9E-18 score: 66.9 coord: 167..283 e-value: 4.2E-22 score: 78.8
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 162..286 score: 31.199999
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 29..158 score: 25.386
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 168..285 e-value: 1.41837E-22 score: 88.7166
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 38..155 e-value: 7.72994E-16 score: 70.6122
None	No IPR available	GENE3D	2.80.10.50		coord: 26..162 e-value: 1.2E-29 score: 105.0
None	No IPR available	GENE3D	2.80.10.50		coord: 163..287 e-value: 2.1E-40 score: 139.3
None	No IPR available	PANTHER	PTHR33453:SF23	RRNA N-GLYCOSIDASE	coord: 36..285
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 36..285
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 37..155
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 162..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M001654	Bhi04M001654	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity