Bhi04G001651 (gene) Wax gourd (B227) v1

Overview
NameBhi04G001651
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionrRNA N-glycosidase
Locationchr4: 55559796 .. 55561382 (+)
RNA-Seq ExpressionBhi04G001651
SyntenyBhi04G001651
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGTTTTAGTAGCTTTGATTATAGCATTATGTATTGCCATTGATGGCATTACAGCAGCAAAAACACCCATTAGTGATCGTTACAAAACCTACATCCAAAATGTTCGCCAAAAATTTGCATCTCAAACTCATAAACAACTCTATGGCATTCCAATGTTGCAGCACTCTTTATCGAATTCAGATCGTTTCCTTCTCATCGATACCGCAAACGTCGCCGGCGACACCATCACTCTTGCAGTCGATGCACAGGACATGAGTGTTGTAGCGTATCTTGCTGGAGACAATGATTCTTACTTTTTCTCAAATGCTCCACTGTTTACTTTTGATATTCTCTTCCCCAACACTCATCAAACACTTTTGAGTTACGACAATACCTTTGAATCCATTGAAAATGCAGCTAATACTACCAGACAAGCAATTCCCCTTGGTCTCAACCCATCAAACTCAGCCATCTCCAACTTGTTTCATTACCGTCCCGATCTCGCCGCTGTTTCGTTTCTCGTCGTCTTTCAAATGATCTTCGAAGCCGCCAAATTCAAGTTCATCGAGCAAACCATTGTTAACTGCATCAAGAATGGAGAAAGCTTCACACCTAACCTTGCCATTGTGAGCTTAGAGGACAATTGGTCTCAACTTTCTTTACAAATCCAATCATCAACTTCTTTACAAGGACTATTTGGGAGTAGTGTAATGCTGCACAACTCGAAAAATGAAGCTATTGAAGTGGATAGTATATACTTTCCAATCATACTTAATAATCTTGCCTTGCAATTATACCATTGCAATACCCAAGATTACATCAAGCTGCCTAGTCTTGTCGAGAATTCTCGATGTTACGTCGAAGAACATACCACACGTATTAGCGGTCGAGATGGACTTTGTGCCGATGTAGCTGATGGTTCTCATGTTATTTCGTTCCCATGTCGACAACAAGCAAACCAACAATGGACGTTTTATAGCGATCGTACGATTCGATCCTTAGGCAAATGTTTGATCCCAAGTAAGTCCTCTTCCAATCCTCTGGCTGTGATTTACGATTGTAGTAAGGTCTCTCAACAAGATGCTACTTGGGAGGTTTCTCTTAGTGGGACAATCATGAACACATATAATGACAGTGTTTTGACATCAAACAATGGAAGCACTTTGACAATGGAGGATAATGCATATGATGGAAATCAAGGGTGGAGAGTTGGGAATTTTGTGGATCCAATTGTAACTTCAATTATTGGGGTTAAAGAGATGTGTTTGGAAGCTACAGAGGGAAATACAAATATTTGGTTAGAAGAATGTGTGAAGGACAAAATAGAGCAATCTTGGACACTTTATAGTGATGGAAGTATTAGAGTGAATAATAATCATAGTCTATGTGTAACAGCTTCAATTCATTCAAGACAGCGTATTGTAATTCTTAATTGCAATGGCTTAGCTACACAACGTTGGGTTTTCAAGGCTGATGGCACAATCTCAACCCCAAAAGATGACGGTTTGGTTATGGATGTTGCACAAAATGATGTCGATCTTAAGAGAATTGTTTTGTATCCTCGACATGGTGGGGTTAATCAACAATGGGTTGTTTTCTACTAA

mRNA sequence

ATGAGAGTTTTAGTAGCTTTGATTATAGCATTATGTATTGCCATTGATGGCATTACAGCAGCAAAAACACCCATTAGTGATCGTTACAAAACCTACATCCAAAATGTTCGCCAAAAATTTGCATCTCAAACTCATAAACAACTCTATGGCATTCCAATGTTGCAGCACTCTTTATCGAATTCAGATCGTTTCCTTCTCATCGATACCGCAAACGTCGCCGGCGACACCATCACTCTTGCAGTCGATGCACAGGACATGAGTGTTGTAGCGTATCTTGCTGGAGACAATGATTCTTACTTTTTCTCAAATGCTCCACTGTTTACTTTTGATATTCTCTTCCCCAACACTCATCAAACACTTTTGAGTTACGACAATACCTTTGAATCCATTGAAAATGCAGCTAATACTACCAGACAAGCAATTCCCCTTGGTCTCAACCCATCAAACTCAGCCATCTCCAACTTGTTTCATTACCGTCCCGATCTCGCCGCTGTTTCGTTTCTCGTCGTCTTTCAAATGATCTTCGAAGCCGCCAAATTCAAGTTCATCGAGCAAACCATTGTTAACTGCATCAAGAATGGAGAAAGCTTCACACCTAACCTTGCCATTGTGAGCTTAGAGGACAATTGGTCTCAACTTTCTTTACAAATCCAATCATCAACTTCTTTACAAGGACTATTTGGGAGTAGTGTAATGCTGCACAACTCGAAAAATGAAGCTATTGAAGTGGATAGTATATACTTTCCAATCATACTTAATAATCTTGCCTTGCAATTATACCATTGCAATACCCAAGATTACATCAAGCTGCCTAGTCTTGTCGAGAATTCTCGATGTTACGTCGAAGAACATACCACACGTATTAGCGGTCGAGATGGACTTTGTGCCGATGTAGCTGATGGTTCTCATGTTATTTCGTTCCCATGTCGACAACAAGCAAACCAACAATGGACGTTTTATAGCGATCGTACGATTCGATCCTTAGGCAAATGTTTGATCCCAAGTAAGTCCTCTTCCAATCCTCTGGCTGTGATTTACGATTGTAGTAAGGTCTCTCAACAAGATGCTACTTGGGAGGTTTCTCTTAGTGGGACAATCATGAACACATATAATGACAGTGTTTTGACATCAAACAATGGAAGCACTTTGACAATGGAGGATAATGCATATGATGGAAATCAAGGGTGGAGAGTTGGGAATTTTGTGGATCCAATTGTAACTTCAATTATTGGGGTTAAAGAGATGTGTTTGGAAGCTACAGAGGGAAATACAAATATTTGGTTAGAAGAATGTGTGAAGGACAAAATAGAGCAATCTTGGACACTTTATAGTGATGGAAGTATTAGAGTGAATAATAATCATAGTCTATGTGTAACAGCTTCAATTCATTCAAGACAGCGTATTGTAATTCTTAATTGCAATGGCTTAGCTACACAACGTTGGGTTTTCAAGGCTGATGGCACAATCTCAACCCCAAAAGATGACGGTTTGGTTATGGATGTTGCACAAAATGATGTCGATCTTAAGAGAATTGTTTTGTATCCTCGACATGGTGGGGTTAATCAACAATGGGTTGTTTTCTACTAA

Coding sequence (CDS)

ATGAGAGTTTTAGTAGCTTTGATTATAGCATTATGTATTGCCATTGATGGCATTACAGCAGCAAAAACACCCATTAGTGATCGTTACAAAACCTACATCCAAAATGTTCGCCAAAAATTTGCATCTCAAACTCATAAACAACTCTATGGCATTCCAATGTTGCAGCACTCTTTATCGAATTCAGATCGTTTCCTTCTCATCGATACCGCAAACGTCGCCGGCGACACCATCACTCTTGCAGTCGATGCACAGGACATGAGTGTTGTAGCGTATCTTGCTGGAGACAATGATTCTTACTTTTTCTCAAATGCTCCACTGTTTACTTTTGATATTCTCTTCCCCAACACTCATCAAACACTTTTGAGTTACGACAATACCTTTGAATCCATTGAAAATGCAGCTAATACTACCAGACAAGCAATTCCCCTTGGTCTCAACCCATCAAACTCAGCCATCTCCAACTTGTTTCATTACCGTCCCGATCTCGCCGCTGTTTCGTTTCTCGTCGTCTTTCAAATGATCTTCGAAGCCGCCAAATTCAAGTTCATCGAGCAAACCATTGTTAACTGCATCAAGAATGGAGAAAGCTTCACACCTAACCTTGCCATTGTGAGCTTAGAGGACAATTGGTCTCAACTTTCTTTACAAATCCAATCATCAACTTCTTTACAAGGACTATTTGGGAGTAGTGTAATGCTGCACAACTCGAAAAATGAAGCTATTGAAGTGGATAGTATATACTTTCCAATCATACTTAATAATCTTGCCTTGCAATTATACCATTGCAATACCCAAGATTACATCAAGCTGCCTAGTCTTGTCGAGAATTCTCGATGTTACGTCGAAGAACATACCACACGTATTAGCGGTCGAGATGGACTTTGTGCCGATGTAGCTGATGGTTCTCATGTTATTTCGTTCCCATGTCGACAACAAGCAAACCAACAATGGACGTTTTATAGCGATCGTACGATTCGATCCTTAGGCAAATGTTTGATCCCAAGTAAGTCCTCTTCCAATCCTCTGGCTGTGATTTACGATTGTAGTAAGGTCTCTCAACAAGATGCTACTTGGGAGGTTTCTCTTAGTGGGACAATCATGAACACATATAATGACAGTGTTTTGACATCAAACAATGGAAGCACTTTGACAATGGAGGATAATGCATATGATGGAAATCAAGGGTGGAGAGTTGGGAATTTTGTGGATCCAATTGTAACTTCAATTATTGGGGTTAAAGAGATGTGTTTGGAAGCTACAGAGGGAAATACAAATATTTGGTTAGAAGAATGTGTGAAGGACAAAATAGAGCAATCTTGGACACTTTATAGTGATGGAAGTATTAGAGTGAATAATAATCATAGTCTATGTGTAACAGCTTCAATTCATTCAAGACAGCGTATTGTAATTCTTAATTGCAATGGCTTAGCTACACAACGTTGGGTTTTCAAGGCTGATGGCACAATCTCAACCCCAAAAGATGACGGTTTGGTTATGGATGTTGCACAAAATGATGTCGATCTTAAGAGAATTGTTTTGTATCCTCGACATGGTGGGGTTAATCAACAATGGGTTGTTTTCTACTAA

Protein sequence

MRVLVALIIALCIAIDGITAAKTPISDRYKTYIQNVRQKFASQTHKQLYGIPMLQHSLSNSDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILFPNTHQTLLSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQMIFEAAKFKFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEAIEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLVENSRCYVEEHTTRISGRDGLCADVADGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQDATWEVSLSGTIMNTYNDSVLTSNNGSTLTMEDNAYDGNQGWRVGNFVDPIVTSIIGVKEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTASIHSRQRIVILNCNGLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQWVVFY
Homology
BLAST of Bhi04G001651 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 583.9 bits (1504), Expect = 1.8e-165
Identity = 300/516 (58.14%), Postives = 382/516 (74.03%), Query Frame = 0

Query: 26  SDRYKTYIQNVRQKFASQTHKQLYGIPMLQHSLSNSDRFLLIDTANVAGDTITLAVDAQD 85
           S  YKT+I  VR++  S+T++ LYGIP+L+HSLSNS+RF L+   +   ++ITLA+D +D
Sbjct: 13  SGTYKTFIGRVREELGSETYR-LYGIPVLKHSLSNSNRFYLLTLTSNQDESITLAIDVED 72

Query: 86  MSVVAYL-AGDNDSYFFSNAPLFTFDILFPNTHQTLLSYDNTFESIENAANTTRQAIPLG 145
           M  VAY  AG ++SYFF NAP   F  LF +THQ +L++DNTF+S+ENAA TTRQ I LG
Sbjct: 73  MVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAGTTRQTIVLG 132

Query: 146 LNPSNSAISNLFHYRPDLAAVSFLVVFQMIFEAAKFKFIEQTIVNCIKNGESFTPNLAIV 205
           ++P + AISNLF+  P L  +SFLV+ QM+ EA+KF+FIEQ++    KN ++F P+LAIV
Sbjct: 133 VDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEKTFLPDLAIV 192

Query: 206 SLEDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEAIEVDSIYFPIILNNLALQLYHCNT 265
           SLEDNWS++SLQIQ+STSLQGLFGS V L+NS NE IEVDSIY+PIIL N+ALQLYHC  
Sbjct: 193 SLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANVALQLYHCQV 252

Query: 266 QDYIKLPSLVENSRCYVEEHTTRISGRDGLCADVA-----DGSHVISFPCRQQANQQWTF 325
                      ++ C VE  TTRISGRD LC DVA     DGS +I +PC QQ NQ+WTF
Sbjct: 253 S--------TGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILYPCGQQVNQKWTF 312

Query: 326 YSDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQDATWEVSLSGTIMN-TYNDSVLTSN 385
           +SD T+RSLGKCL  + S    L VIYDCSK++ +D +W+VS+ GTIMN  Y D  LTSN
Sbjct: 313 HSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSN 372

Query: 386 ---NGSTLTMEDNAYDGNQGWRVGNFVDPIVTSIIGVKEMCLEATEGNTNIWLEECVKDK 445
                + LTME N Y  +QGWRVGN+V PI+ SI+G+ +MCLEAT+GNTN+WLEECV ++
Sbjct: 373 KATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECVPNQ 432

Query: 446 IEQSWTLYSDGSIRVNNNHSLCVTASIHSRQR---IVILNCNGLATQRWVFKADGTISTP 505
            EQSW LYSDG+IRV++N  LCVTAS  +      I ILNC+G   QRWVF ADG+ISTP
Sbjct: 433 REQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSISTP 492

Query: 506 KDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQWVVFY 529
            +  L MDVA++DVDLK+I+L+  HG +NQQWV+FY
Sbjct: 493 GNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

BLAST of Bhi04G001651 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 350.5 bits (898), Expect = 3.3e-95
Identity = 210/562 (37.37%), Postives = 326/562 (58.01%), Query Frame = 0

Query: 1   MRVLVA-------LIIALC-IAIDGITAAKTPI------SDRYKTYIQNVRQKFASQTHK 60
           MRV+ A       +++A+C + I GI             S  Y+ ++ N+R+  A+ T+ 
Sbjct: 1   MRVVAAAMLYFYIVVLAICSVGIQGIDYPSVSFNLDGAKSATYRDFLSNLRKTVATGTY- 60

Query: 61  QLYGIPML--QHSLSNSDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNA 120
           ++ G+P+L  +  +    RF+L+   N  G+T+TLAVD  ++ VVA+ +G+ +SYFF +A
Sbjct: 61  EVNGLPVLRRESEVQVKSRFVLVPLTNYNGNTVTLAVDVTNLYVVAF-SGNANSYFFKDA 120

Query: 121 PLFTFDILFPNTHQTLLSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAA 180
                  LF  T Q  LS+   ++++E AANT R++I LG +P + AI++L+H   D  A
Sbjct: 121 TEVQKSNLFVGTKQNTLSFTGNYDNLETAANTRRESIELGPSPLDGAITSLYH--GDSVA 180

Query: 181 VSFLVVFQMIFEAAKFKFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQ 240
            S LVV QM+ EAA+F++IEQ +   ++   SFTPN  ++S+E+NWS +SL+IQ + +  
Sbjct: 181 RSLLVVIQMVSEAARFRYIEQEVRRSLQQATSFTPNALMLSMENNWSSMSLEIQQAGNNV 240

Query: 241 GLFGSSVMLHNSKNEAIEVDSIYFPIILNNLALQLYHCNT---QDYIKLP--------SL 300
             F  +V L N  +    VD+      +  +A+ L+ C++    + I++P          
Sbjct: 241 SPFFGTVQLLNYDHTHRLVDNFEELYKITGIAILLFRCSSPSNDNAIRMPLDLAGEDNKY 300

Query: 301 VENSRCYVEEHTTR-ISGRDGLCADV-----ADGSHVISFPCRQQANQQWTFYSDRTIRS 360
            +   C +    TR I GRDGLC DV      DG+ +  +PC  Q NQ+WTF SD TIRS
Sbjct: 301 NDGETCTLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRS 360

Query: 361 LGKCLIPSKSSSNPLAVIYDCSKVSQQDATWEVSLSGTIMNTYNDSVLTSNNGST---LT 420
           +GKC+  +  ++    VI++CS  ++    WEV + G+I+N  +  V+T+   ++   L 
Sbjct: 361 MGKCMTANGLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILL 420

Query: 421 MEDNAYDGNQGWRVGNFVDPIVTSIIGVKEMCLEATEGNTNIWLEECVKDKIEQSWTLYS 480
           +EDN Y  +QGW V N V PIV SI+G KEMCL++   N  +W+E+C    ++Q W LY 
Sbjct: 421 LEDNIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYG 480

Query: 481 DGSIRVNNNHSLCVTAS-IHSRQRIVILNCNGLATQRWVFKADGTISTPKDDGLVMDVAQ 526
           D +IRVN+   LCVT +  +S+  I+IL C GL +QRW F +DG I  PK    VMDV  
Sbjct: 481 DRTIRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRH-VMDVRA 540

BLAST of Bhi04G001651 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 8.1e-94
Identity = 214/531 (40.30%), Postives = 304/531 (57.25%), Query Frame = 0

Query: 26  SDRYKTYIQNVRQKFASQTHKQLYGIPML--QHSLSNSDRFLLIDTANVAGDTITLAVDA 85
           +D Y+ +++ +++K     H   + +P+L  +  +S+S+RF+L+   N +GDT+TLA+D 
Sbjct: 44  ADTYEPFLRALQEKVILGNH-TAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDV 103

Query: 86  QDMSVVAYLAGDNDSYFFSNAPLFTFDILFPNTHQTLLSYDNTFESIENAANTTRQAIPL 145
            ++ VVA+ + +  SYFFS +     D LF +T Q  L++   + S+E      R  IPL
Sbjct: 104 VNLYVVAF-SSNGKSYFFSGSTAVQRDNLFVDTTQEELNFTGNYTSLERQVGFGRVYIPL 163

Query: 146 GLNPSNSAISNLFHYRPDLA-----AVSFLVVFQMIFEAAKFKFIEQTIVNCIKNGESFT 205
           G    + AIS+L  Y          A   LVV QM+ EAA+F++IE  I   I +   FT
Sbjct: 164 GPKSLDQAISSLRTYTLTAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFT 223

Query: 206 PNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEAIEVDSIYFPIILNNLALQ 265
           P+L ++S+E+NWS +S +IQ +    G+F   V L + +N +IEV +      L  +A+ 
Sbjct: 224 PDLLMLSMENNWSSMSSEIQQAQP-GGIFAGVVQLRDERNNSIEVTNFRRLFELTYIAVL 283

Query: 266 LYHC----------NTQD--YIKLPSL---VENSRCYVEEHTTRISGRDGLCADV----- 325
           LY C          N  D   IK+P          C V E T RISG DGLC DV     
Sbjct: 284 LYGCAPVTSSSYSNNAIDAQIIKMPVFRGGEYEKVCSVVEVTRRISGWDGLCVDVRYGHY 343

Query: 326 ADGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQDATW 385
            DG+ V   PC  + NQ WTF +D TIR LGKCL  S S      +IYDC+ V  +   W
Sbjct: 344 IDGNPVQLRPCGNECNQLWTFRTDGTIRWLGKCLTASSS-----VMIYDCNTVPPEATKW 403

Query: 386 EVSLSGTIMNTYNDSVLT---SNNGSTLTMEDNAYDGNQGWRVGNFVDPIVTSIIGVKEM 445
            VS+ GTI N ++  VLT   +  G+ L++E+N +   QGW VG+ V+P+VT I+G K+M
Sbjct: 404 VVSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQM 463

Query: 446 CLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTASIHS-RQRIVILNCN 505
           CL     N  +WLE+CV ++++Q W LY DG+IRVN+N SLCVT+  H     IVIL C 
Sbjct: 464 CLRENGENNFVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCE 523

Query: 506 GLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQWV 526
           G   QRWVF  +GTIS P +  L+MDVAQ DV L++I+LY   G  NQQW+
Sbjct: 524 GSGNQRWVFNTNGTISNP-NAKLLMDVAQRDVSLRKIILYRPTGNPNQQWI 564

BLAST of Bhi04G001651 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 4.4e-92
Identity = 222/569 (39.02%), Postives = 316/569 (55.54%), Query Frame = 0

Query: 2   RVLVALIIALC-IAIDGI---TAAKTPI----------SDRYKTYIQNVRQKFASQTHKQ 61
           ++L  +++A+C + I G    T    P+          +D Y  +++ +++K     H  
Sbjct: 6   KLLYLVVLAICGLGIHGALTHTRVTPPVYPSVSFNLTGADTYGPFLRALQEKVILGNH-T 65

Query: 62  LYGIPML--QHSLSNSDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAP 121
            + +P+L  +  +S+S+RF+L+   N +GDT+TLA+D  ++ VVA+ + +  SYFFS + 
Sbjct: 66  AFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAF-SSNGRSYFFSGST 125

Query: 122 LFTFDILFPNTHQTLLSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLA-- 181
               D LF +T Q  L++   + S+E      R  IPLG      AIS+L  Y       
Sbjct: 126 AVQRDNLFVDTTQEELNFTGNYISLERQVGFGRVYIPLGPKSLAQAISSLRTYTLSAGDT 185

Query: 182 ---AVSFLVVFQMIFEAAKFKFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSS 241
              A   LVV QM+ EAA+F++IE  I   I +   FTP+L ++S+E+NWS +S +IQ +
Sbjct: 186 KPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSEIQQA 245

Query: 242 TSLQGLFGSSVMLHNSKNEAIEVDSIYFPIILNNLALQLYHC----------NTQD--YI 301
               G+F   V L + +N  IEV +      L  +A+ LY C          N  D   I
Sbjct: 246 QP-GGIFPGVVQLRDERNNPIEVTNFRRLFELTYIAVLLYGCAPVTSNSYTNNAIDAQII 305

Query: 302 KLPSLVENSR---CYVEEHTTRISGRDGLCADVADGSHVIS-----FPCRQQANQQWTFY 361
           K+P          C V E T RISG DGLC DV DG ++        PC  + NQ WTF 
Sbjct: 306 KMPVFRGGGYEKVCSVVEVTRRISGWDGLCVDVRDGHYIDGNTVQLGPCGNECNQLWTFR 365

Query: 362 SDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQDATWEVSLSGTIMNTYNDSVLT---S 421
           +D TIR LGKCL  S S      +IYDC+ V  +   W VS  GTI N  +  VLT   +
Sbjct: 366 TDGTIRWLGKCLTTSSS-----VMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQA 425

Query: 422 NNGSTLTMEDNAYDGNQGWRVGNFVDPIVTSIIGVKEMCLEATEGNTNIWLEECVKDKIE 481
             G+ L++E+N +   QGW VG+ V+P+VT I+G K+MCL     N  +WLE+CV +++E
Sbjct: 426 AEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLTENGENNFVWLEDCVLNRVE 485

Query: 482 QSWTLYSDGSIRVNNNHSLCVTASIHS-RQRIVILNCNGLATQRWVFKADGTISTPKDDG 526
           Q W LY DG+IRVN+N SLCVT+  H     IVIL C G   QRWVF  +GTIS P +  
Sbjct: 486 QEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNP-NAK 545

BLAST of Bhi04G001651 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.3e-88
Identity = 211/530 (39.81%), Postives = 304/530 (57.36%), Query Frame = 0

Query: 26  SDRYKTYIQNVRQKFASQTHKQLYGIPML--QHSLSNSDRFLLIDTANVAGDTITLAVDA 85
           S+ Y+ ++Q +R K    +H+  + +P+L  +  +S+SDRF+L+   N +   +TLA+D 
Sbjct: 46  SNEYRHFLQELRGKVILGSHR-AFDLPVLNPESKVSDSDRFVLVRLTNPSRKKVTLAIDV 105

Query: 86  QDMSVVAYLAGDNDSYFFSNAPLFTFDILFPNTHQTLLSYDNTFESIENAANTTRQAIPL 145
               VVA+   D  SYFFS +     + LF +T Q  L++   + S+E+     R  IPL
Sbjct: 106 VTFYVVAFAQNDR-SYFFSGSSEVQRENLFVDTTQEDLNFKGDYTSLEHQVGFGRVYIPL 165

Query: 146 GLNPSNSAISNLFHYRPDLA-----AVSFLVVFQMIFEAAKFKFIEQTIVNCIKNGESFT 205
           G      +IS+L  Y+         A S LVV QM+ EAA+F++I+  I   I + + FT
Sbjct: 166 GPKSLAQSISSLSTYKSSAGDNKRLARSLLVVIQMVSEAARFRYIQLRIQASITDAKEFT 225

Query: 206 PNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEAIEVDSIYFPIILNNLALQ 265
           P+L ++S+E+ WS +S +IQ +    G F   V L + +N  I+V +      L ++A+ 
Sbjct: 226 PDLLMLSMENKWSSMSSEIQQAQP-GGAFAQVVKLLDQRNHPIDVTNFRRLFQLTSVAVL 285

Query: 266 LYHCNTQDYIKLPSLV----------ENSRC-YVEEHTTRISGRDGLCADV-----ADGS 325
           L+ C T    K+P+ +          +  RC  VEE T RI GRDG CA+V      DG+
Sbjct: 286 LHGCPT--VTKMPAYIIKMPVFNGGEDEERCSVVEEVTRRIGGRDGFCAEVKNGDEKDGT 345

Query: 326 HVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQDATWEVSL 385
            V    C +Q+NQQWTF +D TI+SLGKCL  S S      +IY+C  V  +   W VS+
Sbjct: 346 PVQLSSCGEQSNQQWTFSTDGTIQSLGKCLTTSSS-----VMIYNCKVVPPESTKWVVSI 405

Query: 386 SGTIMNTYNDSVLT---SNNGSTLTMEDNAYDGNQGWRVGNFVDPIVTSIIGVKEMCLEA 445
            GTI N  +  VLT   +  G+ +++E N +   QGW VGN V+P+VT I+G ++MCLE 
Sbjct: 406 DGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQGWIVGN-VEPLVTFIVGYEQMCLET 465

Query: 446 TEGNTNIWLEEC-VK--DKIEQSWTLYSDGSIRVNNNHSLCVTA-SIHSRQRIVILNCNG 505
             GN ++ L +C VK   K++Q W LY DG+IRVNN+ SLCVT+    S + I+IL C G
Sbjct: 466 NPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNNDRSLCVTSEGKSSNEPIIILKCLG 525

Query: 506 LATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQWV 526
            A QRWVF  DGTIS P D  LVM V QNDV L++I+L    G  NQQW+
Sbjct: 526 WANQRWVFNTDGTISNP-DSKLVMHVDQNDVPLRKIILSHPSGTSNQQWI 563

BLAST of Bhi04G001651 vs. ExPASy TrEMBL
Match: A0A0A0KN71 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 9.2e-218
Identity = 390/537 (72.63%), Postives = 456/537 (84.92%), Query Frame = 0

Query: 1   MRVLVALIIALCIAIDGITAAKTPISDRYKTYIQNVRQKFASQTHKQLYGIPMLQHSLSN 60
           MRVL+A I+ L IA + +  +K P+ D Y++YIQ +RQKF S+THK LYGIP+L HSLSN
Sbjct: 1   MRVLLAFIVTLSIATNAL--SKRPVVDVYQSYIQTIRQKFGSKTHK-LYGIPVLHHSLSN 60

Query: 61  SDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILFPNTHQTL 120
           SDRF LID  N  GDTIT AVDAQDMSVVAYLAGDNDSYFFSNAP F FDILFP T+Q L
Sbjct: 61  SDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNL 120

Query: 121 LSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQMIFEAAKF 180
           L++DN+F+SIE AANTTR+A PLGL PSN+AI+NLFHY P LA VSFL+VFQM+FE+AKF
Sbjct: 121 LNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKF 180

Query: 181 KFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEA 240
           KFIEQ IVN I NGE+FTP+LA++SLEDNWS+LSL+IQ+S SLQGLFGSSV L+NSKNEA
Sbjct: 181 KFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEA 240

Query: 241 IEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLV----ENSRCYVEEHTTRISGRDGLCA 300
           +EVDSIY+P+IL+NLALQLYHCN QDYIK+P+      +N RCYV+E T RISG+DGLCA
Sbjct: 241 VEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQDGLCA 300

Query: 301 DVA-DGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQD 360
           DVA DGSHVIS PC QQANQQWTF+ D TIRS  KCLIP+KS +NPLAVI +C+KVSQ+D
Sbjct: 301 DVARDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQED 360

Query: 361 ATWEVSLSGTIMNTYNDSVLTSNNG---STLTMEDNAYDGNQGWRVGNFVDPIVTSIIGV 420
           ATW+VS+SGTIMN   D VLTS NG   ++L+M+ N Y GNQGWRVGN+V+PI+ SIIGV
Sbjct: 361 ATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGV 420

Query: 421 KEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTA-SIHSRQRIVIL 480
           K+MCLEATE NTNIWLEECVK+KIEQSW ++SDGSIRVNN+HSLC+TA SI S+QRIVI 
Sbjct: 421 KQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQRIVIA 480

Query: 481 NCNGLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQWVVFY 529
            CNGLA+QRWV KADGTISTPK +GLVMDVAQ++VDLK IVLYPR   V+Q WV  Y
Sbjct: 481 KCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 534

BLAST of Bhi04G001651 vs. ExPASy TrEMBL
Match: A0A5A7V3Q8 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00010 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.1e-215
Identity = 390/537 (72.63%), Postives = 452/537 (84.17%), Query Frame = 0

Query: 1   MRVLVALIIALCIAIDGITAAKTPISDRYKTYIQNVRQKFASQTHKQLYGIPMLQHSLSN 60
           MRVL+A ++ L  A + +  +K P+ D Y++YIQ +RQKF S+THK LYGIP+LQHSLSN
Sbjct: 1   MRVLLAFVVTLSFATNAL--SKRPVVDVYQSYIQTIRQKFGSKTHK-LYGIPVLQHSLSN 60

Query: 61  SDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILFPNTHQTL 120
           SDRF LIDT N  GDTITLAVDAQDMSVVAYLAG++DSYFFSNAP F FDILFPNT+Q L
Sbjct: 61  SDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNTNQNL 120

Query: 121 LSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQMIFEAAKF 180
           L++D++F+SIE AANTTRQA PLG   SN+AI+NLFHY   LA VSFL+VFQMIFE+AKF
Sbjct: 121 LNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFESAKF 180

Query: 181 KFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEA 240
            FIEQ IVN IK GE+FTP+LA++SLEDNWS+LSLQIQ+S SLQGLFGSSV L+NSKNEA
Sbjct: 181 NFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNSKNEA 240

Query: 241 IEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLV----ENSRCYVEEHTTRISGRDGLCA 300
           IEVDSIY+P+IL+NLALQLYHCN QDYIK+P+      +N RCYV+E T RISGRDGLC 
Sbjct: 241 IEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGRDGLCV 300

Query: 301 DVA-DGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQD 360
           DVA DGS VIS PC QQANQ+WTF  D TIRS  KCLIP+KS +NP AVI DC+KVSQQD
Sbjct: 301 DVARDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVILDCNKVSQQD 360

Query: 361 ATWEVSLSGTIMNTYNDSVLTSNNG---STLTMEDNAYDGNQGWRVGNFVDPIVTSIIGV 420
           ATW+VS+SGTIMN   D VLTSNNG   ++L+M+ N Y GNQGWRVGNFV+P++ SIIGV
Sbjct: 361 ATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVEPLIVSIIGV 420

Query: 421 KEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTA-SIHSRQRIVIL 480
           K+MCLEATE NTNIWLEECVK+KIEQSW +YSDGSIRVNN+HSLCVTA SI  +QRIVI 
Sbjct: 421 KQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSIEPKQRIVIG 480

Query: 481 NCNGLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQWVVFY 529
            CNGLA+QRWV  ADGTISTPK++GLVMDVAQ++VDLK IVLYPR   V+QQWV  Y
Sbjct: 481 KCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQQWVALY 534

BLAST of Bhi04G001651 vs. ExPASy TrEMBL
Match: A0A1S3CIM5 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.1e-215
Identity = 390/537 (72.63%), Postives = 452/537 (84.17%), Query Frame = 0

Query: 1   MRVLVALIIALCIAIDGITAAKTPISDRYKTYIQNVRQKFASQTHKQLYGIPMLQHSLSN 60
           MRVL+A ++ L  A + +  +K P+ D Y++YIQ +RQKF S+THK LYGIP+LQHSLSN
Sbjct: 1   MRVLLAFVVTLSFATNAL--SKRPVVDVYQSYIQTIRQKFGSKTHK-LYGIPVLQHSLSN 60

Query: 61  SDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILFPNTHQTL 120
           SDRF LIDT N  GDTITLAVDAQDMSVVAYLAG++DSYFFSNAP F FDILFPNT+Q L
Sbjct: 61  SDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNTNQNL 120

Query: 121 LSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQMIFEAAKF 180
           L++D++F+SIE AANTTRQA PLG   SN+AI+NLFHY   LA VSFL+VFQMIFE+AKF
Sbjct: 121 LNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFESAKF 180

Query: 181 KFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEA 240
            FIEQ IVN IK GE+FTP+LA++SLEDNWS+LSLQIQ+S SLQGLFGSSV L+NSKNEA
Sbjct: 181 NFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNSKNEA 240

Query: 241 IEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLV----ENSRCYVEEHTTRISGRDGLCA 300
           IEVDSIY+P+IL+NLALQLYHCN QDYIK+P+      +N RCYV+E T RISGRDGLC 
Sbjct: 241 IEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGRDGLCV 300

Query: 301 DVA-DGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQD 360
           DVA DGS VIS PC QQANQ+WTF  D TIRS  KCLIP+KS +NP AVI DC+KVSQQD
Sbjct: 301 DVARDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVILDCNKVSQQD 360

Query: 361 ATWEVSLSGTIMNTYNDSVLTSNNG---STLTMEDNAYDGNQGWRVGNFVDPIVTSIIGV 420
           ATW+VS+SGTIMN   D VLTSNNG   ++L+M+ N Y GNQGWRVGNFV+P++ SIIGV
Sbjct: 361 ATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVEPLIVSIIGV 420

Query: 421 KEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTA-SIHSRQRIVIL 480
           K+MCLEATE NTNIWLEECVK+KIEQSW +YSDGSIRVNN+HSLCVTA SI  +QRIVI 
Sbjct: 421 KQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSIEPKQRIVIG 480

Query: 481 NCNGLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQWVVFY 529
            CNGLA+QRWV  ADGTISTPK++GLVMDVAQ++VDLK IVLYPR   V+QQWV  Y
Sbjct: 481 KCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQQWVALY 534

BLAST of Bhi04G001651 vs. ExPASy TrEMBL
Match: B7X8M2 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 5.4e-133
Identity = 270/548 (49.27%), Postives = 361/548 (65.88%), Query Frame = 0

Query: 1   MRVL-VALIIALCIAIDGI-----TAAKTPISDRYKTYIQNVRQKFASQTHKQLYGIPML 60
           MRVL V +++AL + I+GI      +     +D YK++I+N+R++          GIP+L
Sbjct: 3   MRVLAVYIVVALSLTINGIECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGIPIL 62

Query: 61  QHSLSNSDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILFP 120
           +HS+   +RFLL+D  N   +TITLA++ +D    AY A D  SYFF NAP     ++F 
Sbjct: 63  KHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADR-SYFFQNAPPIASYVIFT 122

Query: 121 NTHQTLLSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQMI 180
           +T+Q +++++NTFESIE    TTR   PLG+    ++I +LF +  +    SFLV+ QM+
Sbjct: 123 DTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLIQMV 182

Query: 181 FEAAKFKFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLH 240
            EAAKFKFIEQ +++ I + E FTP LA++SLE+NW+QLSLQ+Q+S SL G+FG SV L+
Sbjct: 183 LEAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSVSLY 242

Query: 241 NSKNEAIEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLV----ENSRCYVEEHTTRISG 300
           NS +E I VDS+Y+PI+  N+A QLY C       +P++      N +C  ++ TTRISG
Sbjct: 243 NSMDEPIGVDSMYYPILTANMAFQLYQCPYGVIRMMPTITMPNQNNEQCSPQQRTTRISG 302

Query: 301 RDGLCADV-----ADGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSSNPLAVI 360
           RDGLC DV     ADGS VI +PC QQ NQQWTFY D TIRSLGKCL  S  SS    VI
Sbjct: 303 RDGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSNVVI 362

Query: 361 YDCSKVSQQDATWEVSLSGTIMNTYNDSVLTSN---NGSTLTMEDNAYDGNQGWRVGNFV 420
            +C  +   D  W VS SGT+MN  +  VLT+N   + + LT E+N +   Q WR+GN+V
Sbjct: 363 TNCDYLRYDDG-WMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRIGNYV 422

Query: 421 DPIVTSIIGVKEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTASI 480
           +PIVT+IIG++ MCLEAT+ +TN+WLE CVK+K +Q W LYSD +IRVNNN +LCV++S 
Sbjct: 423 EPIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCVSSST 482

Query: 481 HSRQR-IVILNCNGLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVL-YPRHGGV 529
            S  + IVI  C+G   QRWVF   GTIS P  +  VMDVAQNDV LK+IVL      G 
Sbjct: 483 DSSSKLIVIRRCDGSINQRWVFTPQGTISNPGYEA-VMDVAQNDVYLKKIVLSSATDKGN 542

BLAST of Bhi04G001651 vs. ExPASy TrEMBL
Match: A0A5D3D0Q4 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G00170 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 4.9e-126
Identity = 244/513 (47.56%), Postives = 346/513 (67.45%), Query Frame = 0

Query: 27  DRYKTYIQNVRQKFASQTHKQLYGIPMLQHSLSNSDRFLLIDTANVAGDTITLAVDAQDM 86
           D YK +I  +RQ+    T K LY IP+L+ SL  + RF+ I+  N   +TI+LA+D  ++
Sbjct: 34  DGYKNFINVIRQRLTENTSK-LYDIPILKSSLPPTQRFMTINIVNQNDETISLAIDKVNL 93

Query: 87  SVVAYLAGDNDSYFFSNAPLFTFDILFPNTHQTLLSYDNTFESIENAANTTRQAIPLGLN 146
            V+ Y   +N SY F +APL  FDI+FP T + LL +++ +ESIE A+ TTR    LGL 
Sbjct: 94  GVLGY-RSNNTSYIFLDAPLVAFDIVFPKTCRVLLGFNSNYESIEKASGTTRLQTLLGLE 153

Query: 147 PSNSAISNLFHYRPDLAAVSFLVVFQMIFEAAKFKFIEQTIVNCIKNGESFTPNLAIVSL 206
           P NSAISNLFHY       SFLV+ QM+ E +KFKFIE++++  +K G +F P LAIVSL
Sbjct: 154 PLNSAISNLFHYHRASIPESFLVILQMVLEGSKFKFIERSVIQSLKYGYNFKPGLAIVSL 213

Query: 207 EDNWSQLSLQIQSSTSLQGLFGSSVMLHNSKNEAIEVDSIYFPIILNNLALQLYHCN-TQ 266
           EDNW +LS Q+Q+S SLQGLFG ++ L++S N+ IEVDSIY+ II  N+A QL+HCN + 
Sbjct: 214 EDNWEKLSSQVQASPSLQGLFGEAIKLYDSNNKVIEVDSIYYAIITTNIAFQLHHCNIST 273

Query: 267 DYIKLPSLVENSRCYVEEHTTRISGRDGLCADVADGSH-----VISFPCRQQANQQWTFY 326
           ++I++PS V +S C V+  T  I+G++G C D ++G H     +I + C  Q+NQ+WTF 
Sbjct: 274 NFIRMPSDVGDS-CNVQTRTATITGQNGFCVDASNGLHQDDNSIILYRCGYQSNQEWTFL 333

Query: 327 SDRTIRSLGKCLIPSKSSSNPLAVIYDCSKVSQQ-DATWEVSLSGTIMNTYNDSVLTSN- 386
           SD+TIR   KCL      ++   V+Y+CS+V ++ +  W V++ GTI N  +  VLT+N 
Sbjct: 334 SDKTIRYSNKCL---TFDTSRFVVLYNCSEVEKKSNIRWNVAIDGTISNPSSGLVLTTNP 393

Query: 387 --NGSTLTMEDNAYDGNQGWRVGNFVDPIVTSIIGVKEMCLEATEGNTNIWLEECVKDKI 446
             N S L +E N +  +QGWRVGN+V+P+  SIIG++EMCLEAT  NTN+WLE+CVK+K 
Sbjct: 394 STNSSQLIVEVNKFTTSQGWRVGNYVEPLTGSIIGLEEMCLEATNNNTNVWLEKCVKNKA 453

Query: 447 EQSWTLYSDGSIRVNNNHSLCVTASIHSRQRIVIL-NCNGLATQRWVFKADGTISTPKDD 506
           EQ W +Y DGSIRVN+  +LCV++S +    ++I+  CNG + QRW F A+G I  P + 
Sbjct: 454 EQYWAVYGDGSIRVNSKRNLCVSSSSNRILALIIIEECNGTSNQRWNFLANGAILNP-ET 513

Query: 507 GLVMDVAQNDVDLKRIVLYPRHGGVNQQWVVFY 529
            +VMDV +  V  K+I+LY + G  NQ+W +FY
Sbjct: 514 KMVMDVYRFMVSRKKIILYRKTGERNQEWALFY 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
U3KRF81.8e-16558.14Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2[more]
P331833.3e-9537.37Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2[more]
Q413588.1e-9440.30Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1[more]
O224154.4e-9239.02Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=... [more]
P935432.3e-8839.81Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KN719.2e-21872.63rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1[more]
A0A5A7V3Q81.1e-21572.63rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G0... [more]
A0A1S3CIM51.1e-21572.63rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1[more]
B7X8M25.4e-13349.27rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1[more]
A0A5D3D0Q44.9e-12647.56rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 138..152
score: 43.86
coord: 167..187
score: 48.5
coord: 27..40
score: 23.12
coord: 76..91
score: 41.45
coord: 201..218
score: 45.18
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 283..399
e-value: 1.3E-13
score: 61.2
coord: 403..527
e-value: 3.2E-22
score: 89.8
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 409..524
e-value: 2.6E-20
score: 73.0
coord: 286..396
e-value: 1.1E-14
score: 54.7
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 402..527
score: 32.627998
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 282..399
score: 23.626499
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 287..396
e-value: 2.56801E-13
score: 64.8342
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 409..524
e-value: 1.07779E-19
score: 82.9386
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 181..266
e-value: 6.2E-24
score: 85.7
NoneNo IPR availableGENE3D2.80.10.50coord: 277..403
e-value: 3.5E-25
score: 90.8
NoneNo IPR availableGENE3D6.10.250.2640coord: 25..58
e-value: 1.3E-11
score: 45.9
NoneNo IPR availableGENE3D2.80.10.50coord: 404..528
e-value: 4.1E-42
score: 144.8
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 358..518
coord: 19..358
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 62..180
e-value: 3.8E-26
score: 93.6
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 27..217
e-value: 1.8E-25
score: 89.7
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 358..518
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 19..358
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 27..262
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 404..525
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 279..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M001651Bhi04M001651mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0035821 modulation of process of other organism
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity