Bhi04G001320 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G001320
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	vesicle-associated membrane protein 727
Location	chr4: 41678286 .. 41681618 (+)
RNA-Seq Expression	Bhi04G001320
Synteny	Bhi04G001320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAGAGACTTGTTCCTAAACGGAGACGGACGGTTAAACTGATCGAGGTTCGGCTTCCTTTATATATATGACCCCTCCCTTTTTCTTTCCCCAGTTTCATGAATTCTTCAAACTGATCGATCTTTTAGCTTCCATCTTACGTGAAGTTGACTAGAGATTTCCAGTTTAAGGTTCGCAATTTCCTCTTTGTTCTTCGATTTTTACTTTCTGATTGGATCGTCGGATTTAATTTTGTTTTTTGTGAGTAATTCTGGAAGAATCTGTTTAGGGTTTGATTTGTTTCCCGTACTCTTTCGTAATCAGCATTTGTTTCATCTCCTCTTCGTTCGTCGTAATGTACAATTATTGTTTTCTTTGTGATTTGGAGTTATTGATTATCTGACGGAATAATTGGGGTTTTTCGTATGCGGTTTGGTGTATTTGATGCAATTGTGTATTGGGTAGTACTTCATTTGATTAATTAGTTATTGTTTTTGATTTCAACTTTACTGCCATAGAAAACAAAATATACAAGTTCCTGGTTCTTTGATTGGCCTTTGTTAATTTGGGCTCAGTTTGTATATACTGCTAGTATGCAAACTTTACAAACAATAGGAATTAACTTAGCTGAATAACCTTCACCCAAGATCTAGGAATTTAAATGATAAGTGTTCATACGAGATTTCAAAAAGAGAAGACCCAATAGGCAACAGTTATAGGTAACTGAGCTTAGTTGCAAATTGAATGAGAATAGATTAACGGAAAGGGTAGCCACGTATCATGTTATTAACTAAAATTTTGGGAATTTTCTCATGTTTCCTACCTCTAGGTTGAGAATGAAATTCAATTAACTATGTTCGTTTAATTTTTTTGTAGGTTACTGCTGCAGTTTGTGAGTGCTACTACTTTGATTCTTCTCAGATAACAGTGAGAGATGAGTCAGAAAGGCCTAATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCAGAACACACATCATTCTCAGGGAATTTTAGTACCATTGCTGTTCAATGCTTACAGAGATTGCCTTCTAATAGCAGCAAGTGCACATACTCATGTGATGGGCATACATTCAACTTTCTTCTTGACAGTGGATTTGGTATTGTGTTTTCTTAAGTTCGGCAATGTACTTGTTTTGACTTCACTGGAACATGTTGCTGAAAATGTTTGATTGATTTGCAGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATATGCCATTTGTATTTCTTGAGCGTGTGAAAGATGACTTTAAACAGCGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAGGATGATGATGATTTATTTCTAGACCGATTCAGTGTTGCATACACTCTTGACAGAGAATTTGGGTATGCCTATGTTTATTATCAATGTTTGAGCCTGTGTCGTACATTTTACCTAACTACCTCTAATTTTCATGATAGGCCAAGGCTTAAGGAGCACATGCAGTACTGCATGAACCACCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAGGTGAAAGGAATTATGATGGATAACATTGAAAAGGTTAGGCTTCTTGTTTTATGAAATCATGCAATTTCTGCTCAAGATTTTCTATTGAGGTTTTGAGAGAGTTTCTTTTGCATAATCACTATTGTGCATAATGTATAAACTTGAAAGTACTTTGCACTCTCTTGGCAAATGTATCGAATCTCATTAAACATGTATCTAGGCATACCCCTGTTGCATTTTGGTATCCTGGTAGGTACATCAGCCAAGTAGCATATTAGCCAAGAGCATGGAACATAGTTTTGCTCCCTTGAAGTAATACAGGCAATATTTTAGATTTCAGTGATTATGTTGCAAGGAAATGAAAAAATAAAAATAAAAAAAATATGACACTAGTGTGTTGCTTGCTTGTTTTCAAAGGATCGGATTTTCATCTGGACCAGTTGAAGAATAATTATGTGAAGAATTTGGTATAGGTCAGTTATGGATAAAGTGGACTGGAATTGATCAAATATCTCACATTGCCCCCTTTTCTGCCCTTCCTTGTTCTCAATACAGCCGTTGGAAATGATTGTGGTGATTTTGGGCAATGTAGCATCACTTTGGCATTTAGAATATGATTAAAATATGAATATTTGATAGCTGACATGAGAACTTGATCTTTTCAATTTTTTAACCGATGAACAAAAGATTTTTCATTACTCGATGTTTTATTTTTGATCAAGTAAAGGAAATTGTTGGACCACCTATGCCGATTAAAGTCACTGAATTGAGATCTTGCTTGGGAGATTGGCAAAAGGAGAAAAAACCATGCCATATTTTTTTGTGCAGTTTAAGTATATATGACTACTTTTGTTTCCTGTAATAAAGAGGGAGAGAGAGATGCTTAGAAGTTTCTTCTGCAAATAAACTGAAAATGAGGAGTTTTCTTCCATTTAGCCGCACTCGCCGCCACTAAGTTGTTCGTACCTAAGACGAGGGAGATTTGGGGATTGGAAATCTGGAACTTATCTTTCTCAGCTGTTCTAAATAGATATGATATCAATTTCCTGCTTCTCTTGCAGGTTTTGGATCGTGGTGAACGGATCGAGCTTCTGGTGGACAAAACAGAAAACCTACAATTTCAGGTATGCATCATTTAAACTATCAGTTGAGAACTTTGAATGGGAACTAGGAAGTTGAAGATAATATAAATCATATACCTTTCTTGTTTAACATTCGGATTTCTGTTTAAATTTAGGCTGATAACTTTCATCGACAAGGAAGGCAACTTCGCCGAAAAATGTGGCTTCAGAGTTTACAAATGAAGCTGATGGTTGGGGGAGGAATCTTGATCTTGATCATCATACTTTGGTTTATTGTATGCGGTGGTTTCAAATGTTGATTTGGTAAAGTTAGTAACAGACGAGTGTTTTCAGTTGGCTATGTATGCAAGGAAACTGTTACCAAATCTATATATATATGATATGCCTTGTCTCTTATTCTAATTTGTAATTAGCATTCTATAATTAAATCACCTGTTATTTTTTTTTTTTGTTGGGTTTCTTCTATCTGTTTTGTGTTGAAGAATCTGTGTTGAAAATGAAATTACCTTACACTCTGTTTAATTATACGGTCAATTTGGTTGGCGTTACTATTA

mRNA sequence

TTCAGAGACTTGTTCCTAAACGGAGACGGACGGTTAAACTGATCGAGTTGACTAGAGATTTCCAGTTTAAGGTTACTGCTGCAGTTTGTGAGTGCTACTACTTTGATTCTTCTCAGATAACAGTGAGAGATGAGTCAGAAAGGCCTAATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCAGAACACACATCATTCTCAGGGAATTTTAGTACCATTGCTGTTCAATGCTTACAGAGATTGCCTTCTAATAGCAGCAAGTGCACATACTCATGTGATGGGCATACATTCAACTTTCTTCTTGACAGTGGATTTGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATATGCCATTTGTATTTCTTGAGCGTGTGAAAGATGACTTTAAACAGCGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAGGATGATGATGATTTATTTCTAGACCGATTCAGTGTTGCATACACTCTTGACAGAGAATTTGGGCCAAGGCTTAAGGAGCACATGCAGTACTGCATGAACCACCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAGGTGAAAGGAATTATGATGGATAACATTGAAAAGGTTTTGGATCGTGGTGAACGGATCGAGCTTCTGGTGGACAAAACAGAAAACCTACAATTTCAGGCTGATAACTTTCATCGACAAGGAAGGCAACTTCGCCGAAAAATGTGGCTTCAGAGTTTACAAATGAAGCTGATGGTTGGGGGAGGAATCTTGATCTTGATCATCATACTTTGGTTTATTGTATGCGGTGGTTTCAAATGTTGATTTGGTAAAGTTAGTAACAGACGAGTGTTTTCAGTTGGCTATGTATGCAAGGAAACTGTTACCAAATCTATATATATATGATATGCCTTGTCTCTTATTCTAATTTGTAATTAGCATTCTATAATTAAATCACCTGTTATTTTTTTTTTTTGTTGGGTTTCTTCTATCTGTTTTGTGTTGAAGAATCTGTGTTGAAAATGAAATTACCTTACACTCTGTTTAATTATACGGTCAATTTGGTTGGCGTTACTATTA

Coding sequence (CDS)

ATGAGTCAGAAAGGCCTAATATATAGCTTTGTTGCTAAAGGAACTGTTGTTTTAGCAGAACACACATCATTCTCAGGGAATTTTAGTACCATTGCTGTTCAATGCTTACAGAGATTGCCTTCTAATAGCAGCAAGTGCACATACTCATGTGATGGGCATACATTCAACTTTCTTCTTGACAGTGGATTTGTTTTTCTGGCTGTTGCGGATGAATCAGTTGGAAGGAATATGCCATTTGTATTTCTTGAGCGTGTGAAAGATGACTTTAAACAGCGTTATGGTTCAAGTATCAAGGATGAGAACCCTCACCCACTTGCAGATGATGAGGATGATGATGATTTATTTCTAGACCGATTCAGTGTTGCATACACTCTTGACAGAGAATTTGGGCCAAGGCTTAAGGAGCACATGCAGTACTGCATGAACCACCCAGAAGAAATGAGTAAACTTTCCAAGCTGAAGGCTCAAATAACAGAGGTGAAAGGAATTATGATGGATAACATTGAAAAGGTTTTGGATCGTGGTGAACGGATCGAGCTTCTGGTGGACAAAACAGAAAACCTACAATTTCAGGCTGATAACTTTCATCGACAAGGAAGGCAACTTCGCCGAAAAATGTGGCTTCAGAGTTTACAAATGAAGCTGATGGTTGGGGGAGGAATCTTGATCTTGATCATCATACTTTGGTTTATTGTATGCGGTGGTTTCAAATGTTGA

Protein sequence

MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLDSGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFSVAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIELLVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC

Homology

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BLAST of Bhi04G001320 vs. TAIR 10
Match: AT3G54300.1 (vesicle-associated membrane protein 727 )

HSP 1 Score: 402.5 bits (1033), Expect = 2.3e-112
Identity = 198/238 (83.19%), Postives = 220/238 (92.44%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQ+LP+NSSK TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR++PFVFLERVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W + CGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

[Close]

BLAST of Bhi04G001320 vs. TAIR 10
Match: AT3G54300.2 (vesicle-associated membrane protein 727 )

HSP 1 Score: 402.5 bits (1033), Expect = 2.3e-112
Identity = 198/238 (83.19%), Postives = 220/238 (92.44%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQ+LP+NSSK TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR++PFVFLERVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W + CGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

[Close]

BLAST of Bhi04G001320 vs. TAIR 10
Match: AT2G32670.1 (vesicle-associated membrane protein 725 )

HSP 1 Score: 276.9 bits (707), Expect = 1.5e-74
Identity = 136/238 (57.14%), Postives = 182/238 (76.47%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+L E+T F GNF+ +A QCLQ+LPS+++K TY+CDGHTFN+L++
Sbjct: 66  MGQQNLIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 125

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA ESVGR +P  FLERVK+DF +RYG                         +
Sbjct: 126 NGFTYCVVAVESVGRQIPMAFLERVKEDFNKRYGGGKATT-------------------A 185

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L+REFG +LKEHMQYC++HP+E+SKL+K+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 186 QANSLNREFGSKLKEHMQYCVDHPDEISKLAKVKAQVTEVKGVMMENIEKVLDRGEKIEL 245

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG ++RRKMW +++++KL+V G I+ LI+I+   VCGGFKC
Sbjct: 246 LVDKTENLRSQAQDFRTQGTKIRRKMWFENMKIKLIVLGIIITLILIIILSVCGGFKC 284

[Close]

BLAST of Bhi04G001320 vs. TAIR 10
Match: AT1G04760.1 (vesicle-associated membrane protein 726 )

HSP 1 Score: 271.9 bits (694), Expect = 4.7e-73
Identity = 134/238 (56.30%), Postives = 179/238 (75.21%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+LAE+T F GNF+++A QCLQ+LPS+++K TY+CDGHTFN+L D
Sbjct: 1   MGQQSLIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLAD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  V  ES GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVVIESAGRQIPMAFLERVKEDFNKRYGGGKAST-------------------A 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYC +HPEE+SKLSK+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 KANSLNKEFGSKLKEHMQYCADHPEEISKLSKVKAQVTEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG +++RK+W +++++KL+V G I+ LI+I+   VC GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTQGTKMKRKLWFENMKIKLIVFGIIVALILIIILSVCHGFKC 219

[Close]

BLAST of Bhi04G001320 vs. TAIR 10
Match: AT2G33120.1 (synaptobrevin-related protein 1 )

HSP 1 Score: 271.2 bits (692), Expect = 8.1e-73
Identity = 133/238 (55.88%), Postives = 180/238 (75.63%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQ+LPS+++K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA +S GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVAVDSAGRQIPMAFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM+HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG Q+RRKMW Q++++KL+V   I+ LI+I+   +CGGF C
Sbjct: 181 LVDKTENLRSQAQDFRTQGTQMRRKMWFQNMKIKLIVLAIIIALILIIILSICGGFNC 219

[Close]

BLAST of Bhi04G001320 vs. ExPASy Swiss-Prot
Match: Q9M376 (Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP727 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 3.3e-111
Identity = 198/238 (83.19%), Postives = 220/238 (92.44%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHT +SGNFSTIAVQCLQ+LP+NSSK TYSCDGHTFNFL+D
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GFVFL VADES GR++PFVFLERVK+DFK+RY +SIK++  HPLAD+++DDDLF DRFS
Sbjct: 61  NGFVFLVVADESTGRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAY LDREFGP LKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGE+IEL
Sbjct: 121 VAYNLDREFGPILKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQAD+F RQGRQLRRKMWLQSLQMKLMV G +   I+I+W + CGGFKC
Sbjct: 181 LVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVWVVACGGFKC 238

[Close]

BLAST of Bhi04G001320 vs. ExPASy Swiss-Prot
Match: O48850 (Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP725 PE=2 SV=2)

HSP 1 Score: 276.9 bits (707), Expect = 2.1e-73
Identity = 136/238 (57.14%), Postives = 182/238 (76.47%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+L E+T F GNF+ +A QCLQ+LPS+++K TY+CDGHTFN+L++
Sbjct: 66  MGQQNLIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 125

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA ESVGR +P  FLERVK+DF +RYG                         +
Sbjct: 126 NGFTYCVVAVESVGRQIPMAFLERVKEDFNKRYGGGKATT-------------------A 185

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L+REFG +LKEHMQYC++HP+E+SKL+K+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 186 QANSLNREFGSKLKEHMQYCVDHPDEISKLAKVKAQVTEVKGVMMENIEKVLDRGEKIEL 245

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG ++RRKMW +++++KL+V G I+ LI+I+   VCGGFKC
Sbjct: 246 LVDKTENLRSQAQDFRTQGTKIRRKMWFENMKIKLIVLGIIITLILIIILSVCGGFKC 284

[Close]

BLAST of Bhi04G001320 vs. ExPASy Swiss-Prot
Match: Q9MAS5 (Putative vesicle-associated membrane protein 726 OS=Arabidopsis thaliana OX=3702 GN=VAMP726 PE=2 SV=2)

HSP 1 Score: 271.9 bits (694), Expect = 6.7e-72
Identity = 134/238 (56.30%), Postives = 179/238 (75.21%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           M Q+ LIYSFVA+GTV+LAE+T F GNF+++A QCLQ+LPS+++K TY+CDGHTFN+L D
Sbjct: 1   MGQQSLIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLAD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  V  ES GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVVIESAGRQIPMAFLERVKEDFNKRYGGGKAST-------------------A 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYC +HPEE+SKLSK+KAQ+TEVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 KANSLNKEFGSKLKEHMQYCADHPEEISKLSKVKAQVTEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG +++RK+W +++++KL+V G I+ LI+I+   VC GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTQGTKMKRKLWFENMKIKLIVFGIIVALILIIILSVCHGFKC 219

[Close]

BLAST of Bhi04G001320 vs. ExPASy Swiss-Prot
Match: P47192 (Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP722 PE=2 SV=2)

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-71
Identity = 133/238 (55.88%), Postives = 180/238 (75.63%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQ+LPS+++K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           +GF +  VA +S GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  NGFTYCVVAVDSAGRQIPMAFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM+HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F  QG Q+RRKMW Q++++KL+V   I+ LI+I+   +CGGF C
Sbjct: 181 LVDKTENLRSQAQDFRTQGTQMRRKMWFQNMKIKLIVLAIIIALILIIILSICGGFNC 219

[Close]

BLAST of Bhi04G001320 vs. ExPASy Swiss-Prot
Match: Q9ZTW3 (Vesicle-associated membrane protein 721 OS=Arabidopsis thaliana OX=3702 GN=VAMP721 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 7.4e-71
Identity = 134/238 (56.30%), Postives = 179/238 (75.21%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           M+Q+ LIYSFVA+GTV+L E T F GNF++IA QCLQ+LPS+++K TY+CDGHTFN+L++
Sbjct: 1   MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVE 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
            GF +  VA +S GR +P  FLERVK+DF +RYG                         +
Sbjct: 61  DGFTYCVVAVDSAGRQIPMSFLERVKEDFNKRYGGG-------------------KAATA 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
            A +L++EFG +LKEHMQYCM+HP+E+SKL+K+KAQ++EVKG+MM+NIEKVLDRGE+IEL
Sbjct: 121 QANSLNKEFGSKLKEHMQYCMDHPDEISKLAKVKAQVSEVKGVMMENIEKVLDRGEKIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENL+ QA +F   G Q+RRKMWLQ++++KL+V   I+ LI+I+   VC GFKC
Sbjct: 181 LVDKTENLRSQAQDFRTTGTQMRRKMWLQNMKIKLIVLAIIIALILIIVLSVCHGFKC 219

[Close]

BLAST of Bhi04G001320 vs. ExPASy TrEMBL
Match: A0A5D3CIL2 (Vesicle-associated membrane protein 727 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G00880 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 3.6e-129
Identity = 235/238 (98.74%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGIL+L IILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

[Close]

BLAST of Bhi04G001320 vs. ExPASy TrEMBL
Match: A0A1S3C3Q2 (vesicle-associated membrane protein 727 OS=Cucumis melo OX=3656 GN=LOC103496513 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 3.6e-129
Identity = 235/238 (98.74%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPRLKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGIL+L IILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFIILWFIVCGGFKC 238

[Close]

BLAST of Bhi04G001320 vs. ExPASy TrEMBL
Match: A0A6J1FGZ7 (vesicle-associated membrane protein 727-like OS=Cucurbita moschata OX=3662 GN=LOC111445601 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 8.1e-129
Identity = 233/238 (97.90%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDED+DDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDEDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPR+KEHMQYCMNHPEEM+KLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRIKEHMQYCMNHPEEMNKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGIL+LIIILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLIIILWFIVCGGFKC 238

[Close]

BLAST of Bhi04G001320 vs. ExPASy TrEMBL
Match: A0A0A0KE69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G045200 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 6.8e-128
Identity = 231/238 (97.06%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKG+VVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGSVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFL+RVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLDRVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGP+LKEHMQYCM+HPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPKLKEHMQYCMSHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGIL+L +ILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILVLFVILWFIVCGGFKC 238

[Close]

BLAST of Bhi04G001320 vs. ExPASy TrEMBL
Match: A0A6J1JYK7 (vesicle-associated membrane protein 727-like OS=Cucurbita maxima OX=3661 GN=LOC111489462 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 8.9e-128
Identity = 231/238 (97.06%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQRLPSNSSKCTYSCDGHTFNFLLD 60
           MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQ+LPSN+SKCTYSCDGHTFNFLLD
Sbjct: 1   MSQKGLIYSFVAKGTVVLAEHTSFSGNFSTIAVQCLQKLPSNNSKCTYSCDGHTFNFLLD 60

Query: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDDDDLFLDRFS 120
           SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDED+DDLFLDRFS
Sbjct: 61  SGFVFLAVADESVGRNMPFVFLERVKDDFKQRYGSSIKDENPHPLADDEDEDDLFLDRFS 120

Query: 121 VAYTLDREFGPRLKEHMQYCMNHPEEMSKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180
           VAYTLDREFGPR+KEHMQYCMNHPEEM+KLSKLKAQITEVKGIMMDNIEKVLDRGERIEL
Sbjct: 121 VAYTLDREFGPRIKEHMQYCMNHPEEMNKLSKLKAQITEVKGIMMDNIEKVLDRGERIEL 180

Query: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGGILILIIILWFIVCGGFKC 239
           LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGG IL+LIIILWFIVCGGFKC
Sbjct: 181 LVDKTENLQFQADNFHRQGRQLRRKMWLQSLQMKLMVGGAILVLIIILWFIVCGGFKC 238

The following BLAST results are available for this feature:

BLAST of Bhi04G001320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54300.1	2.3e-112	83.19	vesicle-associated membrane protein 727	[more]
AT3G54300.2	2.3e-112	83.19	vesicle-associated membrane protein 727	[more]
AT2G32670.1	1.5e-74	57.14	vesicle-associated membrane protein 725	[more]
AT1G04760.1	4.7e-73	56.30	vesicle-associated membrane protein 726	[more]
AT2G33120.1	8.1e-73	55.88	synaptobrevin-related protein 1	[more]

BLAST of Bhi04G001320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M376	3.3e-111	83.19	Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]
O48850	2.1e-73	57.14	Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]
Q9MAS5	6.7e-72	56.30	Putative vesicle-associated membrane protein 726 OS=Arabidopsis thaliana OX=3702...	[more]
P47192	1.1e-71	55.88	Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]
Q9ZTW3	7.4e-71	56.30	Vesicle-associated membrane protein 721 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]

BLAST of Bhi04G001320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CIL2	3.6e-129	98.74	Vesicle-associated membrane protein 727 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3C3Q2	3.6e-129	98.74	vesicle-associated membrane protein 727 OS=Cucumis melo OX=3656 GN=LOC103496513 ...	[more]
A0A6J1FGZ7	8.1e-129	97.90	vesicle-associated membrane protein 727-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0KE69	6.8e-128	97.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G045200 PE=3 SV=1	[more]
A0A6J1JYK7	8.9e-128	97.06	vesicle-associated membrane protein 727-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001388	Synaptobrevin	PRINTS	PR00219	SYNAPTOBREVN	coord: 154..173 score: 50.41 coord: 174..193 score: 42.43 coord: 210..229 score: 26.22
IPR001388	Synaptobrevin	PFAM	PF00957	Synaptobrevin	coord: 148..234 e-value: 7.9E-32 score: 108.9
IPR001388	Synaptobrevin	PROSITE	PS00417	SYNAPTOBREVIN	coord: 167..186
IPR010908	Longin domain	SMART	SM01270	Longin_2	coord: 31..111 e-value: 1.0E-21 score: 88.2
IPR010908	Longin domain	PFAM	PF13774	Longin	coord: 33..100 e-value: 5.6E-18 score: 64.8
IPR010908	Longin domain	PROSITE	PS50859	LONGIN	coord: 6..133 score: 23.71977
IPR010908	Longin domain	CDD	cd14824	Longin	coord: 7..140 e-value: 6.68287E-32 score: 111.194
None	No IPR available	GENE3D	3.30.450.50	Longin domain	coord: 1..111 e-value: 1.1E-38 score: 134.7
None	No IPR available	GENE3D	1.20.5.110		coord: 123..233 e-value: 1.9E-34 score: 119.8
None	No IPR available	PANTHER	PTHR21136	SNARE PROTEINS	coord: 1..238
None	No IPR available	PANTHER	PTHR21136:SF169	VESICLE-ASSOCIATED MEMBRANE PROTEIN 727	coord: 1..238
None	No IPR available	CDD	cd15843	R-SNARE	coord: 149..207 e-value: 4.20942E-29 score: 102.195
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 144..209
IPR042855	v-SNARE, coiled-coil homology domain	PROSITE	PS50892	V_SNARE	coord: 149..209 score: 19.343924
IPR011012	Longin-like domain superfamily	SUPERFAMILY	64356	SNARE-like	coord: 6..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M001320	Bhi04M001320	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle