Bhi04G000874 (gene) Wax gourd (B227) v1

Overview
NameBhi04G000874
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCaM_binding domain-containing protein
Locationchr4: 26859665 .. 26862558 (+)
RNA-Seq ExpressionBhi04G000874
SyntenyBhi04G000874
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAACTGAAAAAGGAAAAGAAAGAAAGAAAGAACAAAAAAGAAACTCTCAATTTCTTTCAAACTGATTCTTTTTCCTTCCTGTTTTATCATCATTCCCATTTCTTCACTCCATGGTTTCTTTCTTTCCTTCCTCAAGGTATGTGTTTCTTGCTTCCTCTCTCTCTCGAATTTCAATTCCTCGTCTTTCATTTTTCGAGAAAGGTAGAGGTGAGGATCGAACTTTCGACTTCAAGAAAGATAATAGGGTATCATATGATCTACTTCGATTTGTTTTCAATCTTTTGCTTTTTTTATGAGTTTTTTCATCGAGACGTTTCCCAAATCTAAGTTGTACATACGTTTCTTTCTTAAAATAAGATATCGAAGCTACCATTTTGTACATTCAAATAGTGGATAGCTTGATCCTTATGTGCAATTGTTGTACTAAATAAGAAAAGAAGAAATCCAATATACCAAATATAAATTTAACTTCTAAATCTTTAATTGCATAACCCAATCCATGAACCAAATATCATCTAAATTCTCCCATGAGATGGAAGGCTTCAAATTTTATGTTAGTTTTCTCCTTTTCTATATTTTTTTTCAACATGAATAAGTGTATTATAGATTATGGCAGTTGTTTTGTGAAGAATAGGATTTGTTAGAAGGAAACTTTTGATGCCTCAATGATCATTTGAAGTTAAGATATGATATGAAAATGCACTCAGATCTTCATTTTGTTGTTTAAGAACAAATATTGATGTTTTTTTTCCCTTATATTATCAAGAACTTACAACCATGGGTATAATTTGGAAGGGTATCTATTCTTTTCTATGAGATCAAGGGTCAAAAATTCAAATTGTCATTTTAATGTATAGATTTTAAGAAAGTAAAACATGTTTTTTTTTTTTTTTTTTGACAATATATGTGGTGAGGGAATCAAACCTCTGATCTCGAGATCAATAATATAAACTTTATTTTAGTTGTGCTAGGTTCATTTTGGCAGATTAAAATGTTTTATAAACCATTTTTCAAAATTTTCTTAGAATGCTTTTACTTCAAGGCACTTTTGTCAAAAAGTGTTTTCCCCCTCTTCATGCTGTTATTGAAACGCAAGGAATGGCGTTTGAAAATGCAGGTAAATGCTAGAACTCTAAAAAAGGCCAAAGTAGCTGAGCTGAATGGCAACAACTAAAGCAAAAGAAAACAGTGGTTTGGGGAAGGAAAGAAGGATCACAGCTCCAAGTTCTGTCACAATTCCTACTCATACTCCAACCAAAAGAACTCCAAAATCATCCCCTAGAGGCCTTATCTCTTCCACTGATCCAAAACTTTCTTCTTCTTCTCCTTCTCCTTCTTCCTCTTCTTCTTCCTCTACTCTTTCTCTTTCCGTTCTCCCCGAAAAACACATTCCGAATTATCTCAAGTCGACAGCCAGCTCCCGCCATGACCATAACTTTAAGCCAGTAACCAGGAGTAAGTCTGGCCCAGCACCAATACCTGAGGGCAAACCTAGTTTGAATAGAAGAAGATCTTTCGATAAACCTCCCTCGACCGCACCACGTTTGGGGAAACCGTTTCGATCGCCTGGACCGAGGAATAGGGCTGCTCATGTTCCGGTTCGGTCGTCCTCTTTTGGGGCTAAACCTTCCACCACCACAATTGTCAATTCTACAAAGCCTGGTTTTCTGGATAGATCATCATCTAAAATCTCTAAGGTGGGAGGGAAACCACAGCAACCAATTCATAGTTTGAAAAGTAGTAATAGTAATGTAAAGAAAAGCTTGAAAAGAGAATCTAGCAATGCTGCTGCTTCTGCTTCTGTTTCTGCTTCTGCTTCTGCTTCTGCTTCAGCTTCAGCAGAAATCGTTCCCAAGACTAAGAATGCTGTTGAACATGTAGATCACAGTCCATCTTTCGTTCTCGGTGTTAACGAAGAGGATTTGAAAAAGATCGAGTGCGAACTCGATCCTTATCTGCCTGATCCAATGCCTGAATTGGACAAACAACTTAGAATCGATCAGGTGGAAAAGAAGGCAGTTTTGGATAAAGATGAGGCTGACACAGAAACCTTCAAAGTAGTTGTTGCTAATAAAGAAGTGGAAGAAATTCCAGAGGCAGCCGAGACTGAAATTTCATTAACGAATCGAGAACGAGACGAAGTGGAAGGAGAAGAGGAGAAACCAGAGAGAATTGCAGTGGAAAAAGAGGAAGTTGTTGGATCAGAGGAAATTAACACAAACAACAAGAATGATCAAGAGAAGAGTGAAACAGATCCTGTTTTAGATGGGGAGAGTTACAAGGAAGAAAATGATCACAAGGCGGCCGAGACAACAGAGAAAGAGGCCGAAGCGACCGAGAAAGCGGCAGCGGCAGTGGTAGCAAAACCGCGACAAGGAGGACCAGGGAGAAAAGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGTAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGATTATGAATCTTCATCTAAATGATTCATAAATGCCTCCCATTTTCTCATTCTTCATTGATTAATTTAACTTACCCATAAAAACTCATTATGGAAAATTTAACAGTACAAGGTCATTTATACACAACAATGTAGAGTTGAGGTTTTTGTTCTTCCTAATTTTCCTTCTTGGTGGTGGGGTATTTTTTTTTTTTTCTTTTTTTCATTTTTTCTTTTTCACAAGGGATTGATCTTTTCTGAATTTCTTATCTTACTTGTATAATAAAATGACACAATTTGGAGAGAAGATTGTATCAGTTTGTTGTTTTTTCTTTTCTTTCTATTCCAGCTGTTGATATTGGGATGTTGTATAAGTGTGAGGTTTAATTATCAAAATACCATAAACTACGAG

mRNA sequence

CAAAAACTGAAAAAGGAAAAGAAAGAAAGAAAGAACAAAAAAGAAACTCTCAATTTCTTTCAAACTGATTCTTTTTCCTTCCTGTTTTATCATCATTCCCATTTCTTCACTCCATGGTTTCTTTCTTTCCTTCCTCAAGGTAAATGCTAGAACTCTAAAAAAGGCCAAAGTAGCTGAGCTGAATGGCAACAACTAAAGCAAAAGAAAACAGTGGTTTGGGGAAGGAAAGAAGGATCACAGCTCCAAGTTCTGTCACAATTCCTACTCATACTCCAACCAAAAGAACTCCAAAATCATCCCCTAGAGGCCTTATCTCTTCCACTGATCCAAAACTTTCTTCTTCTTCTCCTTCTCCTTCTTCCTCTTCTTCTTCCTCTACTCTTTCTCTTTCCGTTCTCCCCGAAAAACACATTCCGAATTATCTCAAGTCGACAGCCAGCTCCCGCCATGACCATAACTTTAAGCCAGTAACCAGGAGTAAGTCTGGCCCAGCACCAATACCTGAGGGCAAACCTAGTTTGAATAGAAGAAGATCTTTCGATAAACCTCCCTCGACCGCACCACGTTTGGGGAAACCGTTTCGATCGCCTGGACCGAGGAATAGGGCTGCTCATGTTCCGGTTCGGTCGTCCTCTTTTGGGGCTAAACCTTCCACCACCACAATTGTCAATTCTACAAAGCCTGGTTTTCTGGATAGATCATCATCTAAAATCTCTAAGGTGGGAGGGAAACCACAGCAACCAATTCATAGTTTGAAAAGTAGTAATAGTAATGTAAAGAAAAGCTTGAAAAGAGAATCTAGCAATGCTGCTGCTTCTGCTTCTGTTTCTGCTTCTGCTTCTGCTTCTGCTTCAGCTTCAGCAGAAATCGTTCCCAAGACTAAGAATGCTGTTGAACATGTAGATCACAGTCCATCTTTCGTTCTCGGTGTTAACGAAGAGGATTTGAAAAAGATCGAGTGCGAACTCGATCCTTATCTGCCTGATCCAATGCCTGAATTGGACAAACAACTTAGAATCGATCAGGTGGAAAAGAAGGCAGTTTTGGATAAAGATGAGGCTGACACAGAAACCTTCAAAGTAGTTGTTGCTAATAAAGAAGTGGAAGAAATTCCAGAGGCAGCCGAGACTGAAATTTCATTAACGAATCGAGAACGAGACGAAGTGGAAGGAGAAGAGGAGAAACCAGAGAGAATTGCAGTGGAAAAAGAGGAAGTTGTTGGATCAGAGGAAATTAACACAAACAACAAGAATGATCAAGAGAAGAGTGAAACAGATCCTGTTTTAGATGGGGAGAGTTACAAGGAAGAAAATGATCACAAGGCGGCCGAGACAACAGAGAAAGAGGCCGAAGCGACCGAGAAAGCGGCAGCGGCAGTGGTAGCAAAACCGCGACAAGGAGGACCAGGGAGAAAAGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGTAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGATTATGAATCTTCATCTAAATGATTCATAAATGCCTCCCATTTTCTCATTCTTCATTGATTAATTTAACTTACCCATAAAAACTCATTATGGAAAATTTAACAGTACAAGGTCATTTATACACAACAATGTAGAGTTGAGGTTTTTGTTCTTCCTAATTTTCCTTCTTGGTGGTGGGGTATTTTTTTTTTTTTCTTTTTTTCATTTTTTCTTTTTCACAAGGGATTGATCTTTTCTGAATTTCTTATCTTACTTGTATAATAAAATGACACAATTTGGAGAGAAGATTGTATCAGTTTGTTGTTTTTTCTTTTCTTTCTATTCCAGCTGTTGATATTGGGATGTTGTATAAGTGTGAGGTTTAATTATCAAAATACCATAAACTACGAG

Coding sequence (CDS)

ATGGCAACAACTAAAGCAAAAGAAAACAGTGGTTTGGGGAAGGAAAGAAGGATCACAGCTCCAAGTTCTGTCACAATTCCTACTCATACTCCAACCAAAAGAACTCCAAAATCATCCCCTAGAGGCCTTATCTCTTCCACTGATCCAAAACTTTCTTCTTCTTCTCCTTCTCCTTCTTCCTCTTCTTCTTCCTCTACTCTTTCTCTTTCCGTTCTCCCCGAAAAACACATTCCGAATTATCTCAAGTCGACAGCCAGCTCCCGCCATGACCATAACTTTAAGCCAGTAACCAGGAGTAAGTCTGGCCCAGCACCAATACCTGAGGGCAAACCTAGTTTGAATAGAAGAAGATCTTTCGATAAACCTCCCTCGACCGCACCACGTTTGGGGAAACCGTTTCGATCGCCTGGACCGAGGAATAGGGCTGCTCATGTTCCGGTTCGGTCGTCCTCTTTTGGGGCTAAACCTTCCACCACCACAATTGTCAATTCTACAAAGCCTGGTTTTCTGGATAGATCATCATCTAAAATCTCTAAGGTGGGAGGGAAACCACAGCAACCAATTCATAGTTTGAAAAGTAGTAATAGTAATGTAAAGAAAAGCTTGAAAAGAGAATCTAGCAATGCTGCTGCTTCTGCTTCTGTTTCTGCTTCTGCTTCTGCTTCTGCTTCAGCTTCAGCAGAAATCGTTCCCAAGACTAAGAATGCTGTTGAACATGTAGATCACAGTCCATCTTTCGTTCTCGGTGTTAACGAAGAGGATTTGAAAAAGATCGAGTGCGAACTCGATCCTTATCTGCCTGATCCAATGCCTGAATTGGACAAACAACTTAGAATCGATCAGGTGGAAAAGAAGGCAGTTTTGGATAAAGATGAGGCTGACACAGAAACCTTCAAAGTAGTTGTTGCTAATAAAGAAGTGGAAGAAATTCCAGAGGCAGCCGAGACTGAAATTTCATTAACGAATCGAGAACGAGACGAAGTGGAAGGAGAAGAGGAGAAACCAGAGAGAATTGCAGTGGAAAAAGAGGAAGTTGTTGGATCAGAGGAAATTAACACAAACAACAAGAATGATCAAGAGAAGAGTGAAACAGATCCTGTTTTAGATGGGGAGAGTTACAAGGAAGAAAATGATCACAAGGCGGCCGAGACAACAGAGAAAGAGGCCGAAGCGACCGAGAAAGCGGCAGCGGCAGTGGTAGCAAAACCGCGACAAGGAGGACCAGGGAGAAAAGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGTAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGATTATGAATCTTCATCTAAATGA

Protein sequence

MATTKAKENSGLGKERRITAPSSVTIPTHTPTKRTPKSSPRGLISSTDPKLSSSSPSPSSSSSSSTLSLSVLPEKHIPNYLKSTASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRRSFDKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFGAKPSTTTIVNSTKPGFLDRSSSKISKVGGKPQQPIHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKTKNAVEHVDHSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEKKAVLDKDEADTETFKVVVANKEVEEIPEAAETEISLTNRERDEVEGEEEKPERIAVEKEEVVGSEEINTNNKNDQEKSETDPVLDGESYKEENDHKAAETTEKEAEATEKAAAAVVAKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Homology
BLAST of Bhi04G000874 vs. TAIR 10
Match: AT5G24880.1 (BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (TAIR:AT5G10660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 67.0 bits (162), Expect = 4.4e-11
Identity = 141/465 (30.32%), Postives = 204/465 (43.87%), Query Frame = 0

Query: 32  TKRTPKSSPRGLISSTDPKLSSSSPSPSSSSSSSTLSLSVLPEKHIPNYLKSTASSRHDH 91
           T  T K+ P     S    L      P S++SS   S    PEK +PNYLK T SSR D 
Sbjct: 4   TSSTTKAKPDNQNLSRTKSLGRKPKQPLSTASSEDGSSDQKPEKPLPNYLKPTISSRPDA 63

Query: 92  NFKPVTRSKSGPAPIPEGKPSLNRRRSFDKPPST--------APRLGKPFR-SPGPRNRA 151
             K + ++ +      +    L RRRSFD+PPS+        +PR+ K    SP      
Sbjct: 64  G-KLLKKNNNS----LDDNQKLLRRRSFDRPPSSLTSPSTSASPRIQKSLNVSPSRSRDR 123

Query: 152 AHVP-------VRSSSF--------GAKPSTTTIVNSTKPGFLDRSSSKISKVGGKPQQP 211
             VP       +RSSSF        G   + +  V   K G    S+S  SK  G     
Sbjct: 124 PAVPREKPVTALRSSSFHGSRNVPKGGNTAKSPPVAPKKSGLNSSSTSSKSKKEG----- 183

Query: 212 IHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKTKNAVEHVDH---SP 271
                S N  +KK+  +E +  +AS S     SA      EI+    + ++  DH    P
Sbjct: 184 -----SENVRIKKASDKEIALDSASMS-----SAQEDHQEEILKVESDHLQVSDHDIEEP 243

Query: 272 SF----------VLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEKKAVLDKDEAD 331
            +          V+  NE   +K E      +  P+ + D    + ++E+K + ++D+ +
Sbjct: 244 KYEKEEKEVQEKVVQANESVEEKAESSGPTPVASPVGK-DCNAVVAELEEKLIKNEDDIE 303

Query: 332 TETFKVV-----VANKEVEEIPEAAETEISLTNRERDEVEGEEEKPERIAVEKEEVVGSE 391
            +T ++       ANK  EE     + + + T  + D    E E  E    EKEE V  E
Sbjct: 304 EKTEEMKEQDNNQANKSEEEEDVKKKIDENETPEKVDTESKEVESVEETTQEKEEEVKEE 363

Query: 392 EINTNNKNDQEKSETDPVLDGESYKEENDHKAAETTEKEAEATEKAAAAVVAKPRQGGPG 451
                 + ++EK +       E  +EE   K     EKE    E++A     K ++   G
Sbjct: 364 GKERVEEEEKEKEKVKEDDQKEKVEEEEKEKVKGDEEKEKVKEEESAE---GKKKEVVKG 423

Query: 452 RKESPAMYNHVIEETASKLLEK-RKNKVRALAGAFQTVIDYESSS 454
           +KESP+ YN VI   ASK+ E  RKNKV ALAGAFQTVIDYE+++
Sbjct: 424 KKESPSAYNDVI---ASKMQENPRKNKVLALAGAFQTVIDYETAA 441

BLAST of Bhi04G000874 vs. TAIR 10
Match: AT5G10660.1 (calmodulin-binding protein-related )

HSP 1 Score: 60.8 bits (146), Expect = 3.2e-09
Identity = 137/448 (30.58%), Postives = 194/448 (43.30%), Query Frame = 0

Query: 25  TIPTHTPTKRTPKSSPRGLISSTDPKLSSSSPSPSSSSSSSTLSLSVLPEKHIPNYLKST 84
           T  + T TKR  K+  R       PK  SSS   +++  S   ++     K +PNYLK T
Sbjct: 3   TTSSTTKTKRDNKNLTRSKSLGRKPKPVSSSEPEANADGSDRKTIG----KPLPNYLKPT 62

Query: 85  ASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRRSFDKPPS--TAPRLGKPFRSPGPRNRA 144
            SSR D    PV   +   A   E    L RRRSFD PPS  T+P      RS       
Sbjct: 63  ISSRPD----PVKFLRKNNA--VEENQKLLRRRSFDHPPSSLTSPSTSSAHRSLNTSPAH 122

Query: 145 AHVPVRSSSFGAKPST----TTIVNSTKPGFLDRSSSKISKVGGKPQQPIHSLKSSNSNV 204
            H+  + +    KP T    T+   S++ G    S+ K   V  +    +     S ++ 
Sbjct: 123 PHLRDKPAVPREKPVTGLRSTSFHGSSRGGLRGSSTVKSPPVASRGSSGVKKSGLSGNSS 182

Query: 205 KKSLKRESSNAAASASVSASASASASASAEIVPKTKNAVEHVDHSPSFVLGVNEEDLKKI 264
            KS K  S N               S+  EI P         D SP      +EE++ K+
Sbjct: 183 SKSKKEGSGNV-----------PKKSSGKEISP---------DSSPLASAHEDEEEIVKV 242

Query: 265 ECE--LDPYLPDPMPE-LDKQLRIDQVEKKAVLDKDEADTETFK--VVVANKEVEEIPEA 324
           E +  +  +  +P  E  D+  + D+  ++       A TE  K  ++  +K  E+I E 
Sbjct: 243 ETDVHISDHGEEPKEEDKDQFAQPDESGEEKETSPVAASTEEQKGELIDEDKSTEQIEEP 302

Query: 325 AETEISLTNRERDEVE-----GEEEKPERIAVEKEEVVGSEEINTNNKNDQEKSETDPVL 384
            E E    N   +E E      +EE  E +A   +    +E +N     ++EK E     
Sbjct: 303 KEPENIEENNSEEEEEVKKKSDDEENSETVATTTDM---NEAVNVEESKEEEKEE----- 362

Query: 385 DGESYKEENDHKAA--ETTEKEAEATEKAAAAVVAKPRQGGPGRKESPAMYNHVIEETAS 444
             E  +EE +  AA  ETTE  A+  E        +  Q   G+KESP  YN VI   AS
Sbjct: 363 -AEVKEEEGESSAAKEETTETMAQVEELPEEGTKNEVVQ---GKKESPTAYNDVI---AS 405

Query: 445 KLLE-KRKNKVRALAGAFQTVIDYESSS 454
           K+ E  +KNKV ALAGAFQTVIDYE+++
Sbjct: 423 KMQENSKKNKVLALAGAFQTVIDYETAA 405

BLAST of Bhi04G000874 vs. ExPASy TrEMBL
Match: A0A0A0LS85 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 9.5e-179
Identity = 378/468 (80.77%), Postives = 407/468 (86.97%), Query Frame = 0

Query: 1   MATTKAKENSGLGKERRITAPSSVTIPTHTPTKRTPKSSPRGLISSTDPKLSSSSPSPS- 60
           MATT++KE+ GLGKER+IT PSSVTIP++T TKRTPKSSPRG+ISSTDPK  S SPS + 
Sbjct: 1   MATTRSKESCGLGKERKITPPSSVTIPSNTSTKRTPKSSPRGIISSTDPKFPSFSPSSAP 60

Query: 61  SSSSSSTLSLSVLPEKHIPNYLKSTASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRRSF 120
           SS SS+  SLS+LPEKH+PNYLKSTASSR+DHNFKP+ RSKSGPAPIPEG+PSLNRRRSF
Sbjct: 61  SSPSSAPSSLSILPEKHVPNYLKSTASSRNDHNFKPIARSKSGPAPIPEGRPSLNRRRSF 120

Query: 121 DKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFGAKPS--TTTIVNSTKPGFLDRSSSKI 180
           DKPP T PRLGKPFRSPGPRNR  HVPVRSSSFGAKPS  TTTI +STKPGFL+RSSSK 
Sbjct: 121 DKPPPTVPRLGKPFRSPGPRNRTPHVPVRSSSFGAKPSTTTTTIGHSTKPGFLERSSSKS 180

Query: 181 SKVGGKPQQPIHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKTKNAV 240
           SKVGGKPQ PI SLK+S+S VKKSL+RESSNAAASAS SASASASASASAEIVPK+KNAV
Sbjct: 181 SKVGGKPQPPIQSLKTSSSYVKKSLRRESSNAAASASASASASASASASAEIVPKSKNAV 240

Query: 241 EHVDHSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEKKAVLDKDEADTET 300
           EHVD SPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+R+DQVEKKAVLDKDE DTE 
Sbjct: 241 EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQIRLDQVEKKAVLDKDETDTEI 300

Query: 301 FKVVVANKEVE---EIPEAAETEISLTNRERDEVEGEEEK----PERIAVEKEEVVGSEE 360
            KVVV NKEVE   E P   ETEISL N+E++E EGEE K    P+RI VEKEEVV  EE
Sbjct: 301 LKVVVTNKEVEEQKETPRTVETEISLANQEQNEREGEEPKEEDNPKRIEVEKEEVVALEE 360

Query: 361 INTNNKNDQEKSETDPVLDGESYKEENDH----KAAETTEKEAEATEKAAAAVVAKPRQG 420
            + NNKNDQEKSETD VLDGES KEE+D     KAAETTEKEAE+TEK      AKPRQG
Sbjct: 361 -SINNKNDQEKSETDSVLDGESCKEESDQNSTPKAAETTEKEAESTEK-----TAKPRQG 420

Query: 421 GPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
           GPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 GPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 462

BLAST of Bhi04G000874 vs. ExPASy TrEMBL
Match: A0A5A7VBK4 (Serine/arginine repetitive matrix protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003220 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 2.0e-176
Identity = 376/469 (80.17%), Postives = 405/469 (86.35%), Query Frame = 0

Query: 1   MATTKAKENSGLGKERRITAPSSVTIPTHTPTKRTPKSSPRGLISSTDPKL---SSSSPS 60
           MATT++KE+ GLGKERRIT PSSVTIP +TPTKRTPKSSPRG ISSTDPK+   SSSSPS
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  PSSSSSSSTLSLSVLPEKHIPNYLKSTASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRR 120
           PSS+ +    SLS+LPEK +PNYLKSTASSR+DHNFKP+TRSKSGPAPIPEGKPSLNRRR
Sbjct: 61  PSSAPT----SLSILPEKPVPNYLKSTASSRNDHNFKPITRSKSGPAPIPEGKPSLNRRR 120

Query: 121 SFDKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFGAKPSTTTIV--NSTKPGFLDRSSS 180
           SFDKPP TAPRL KPFRSPGPRNRA HVPVRSSSFGAKPST+T +  +STKPGFL+RSSS
Sbjct: 121 SFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTTIIGHSTKPGFLERSSS 180

Query: 181 KISKVGGKPQQPIHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKTKN 240
           KISKVGGKPQ PI SLK+S+S+VKKSL+RESSN      V+ASASASASASAEIVPKTKN
Sbjct: 181 KISKVGGKPQPPIQSLKTSSSHVKKSLRRESSN------VAASASASASASAEIVPKTKN 240

Query: 241 AVEHVDHSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEKKAVLDKDEADT 300
           AVEHVD SPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+RIDQVEKKA LDKDE DT
Sbjct: 241 AVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQVRIDQVEKKAALDKDETDT 300

Query: 301 ETFKVVVANKEVE--EIPEAAETEISLTNRERDEVE----GEEEKPERIAVEKEEVVGSE 360
           E  KVVV NKEVE  E P  AETEISL NRE++E E     EE+KP+RI VEKEEV+  E
Sbjct: 301 EILKVVVTNKEVEEKETPGTAETEISLANREQNEGEEEEPKEEDKPKRIGVEKEEVLALE 360

Query: 361 EINTNNKNDQEKSETDPVLDGESYKEENDH----KAAETTEKEAEATEKAAAAVVAKPRQ 420
           E + NNKNDQEKSETD VLDGES KEEND     KAAETTEKEAE+TEK      AKPRQ
Sbjct: 361 E-SINNKNDQEKSETDSVLDGESCKEENDQNSTPKAAETTEKEAESTEK-----TAKPRQ 420

Query: 421 GGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
           G PGRKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 GAPGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 453

BLAST of Bhi04G000874 vs. ExPASy TrEMBL
Match: A0A1S3BBU4 (serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488229 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.9e-175
Identity = 374/469 (79.74%), Postives = 402/469 (85.71%), Query Frame = 0

Query: 1   MATTKAKENSGLGKERRITAPSSVTIPTHTPTKRTPKSSPRGLISSTDPKL---SSSSPS 60
           MATT++KE+ GLGKERRIT PSSVTIP +TPTKRTPKSSPRG ISSTDPK+   SSSSPS
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  PSSSSSSSTLSLSVLPEKHIPNYLKSTASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRR 120
           PSS+ +    SLS+LPEK +PNYLKSTASSR+DHNFKP+TRSKSGPAPIPEGKPSLNRRR
Sbjct: 61  PSSAPT----SLSILPEKPVPNYLKSTASSRNDHNFKPITRSKSGPAPIPEGKPSLNRRR 120

Query: 121 SFDKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFGAKPSTTTIV--NSTKPGFLDRSSS 180
           SFDKPP TAPRL KPFRSPGPRNRA HVPVRSSSFGAKPST+T +  +STKPGFL+RSSS
Sbjct: 121 SFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTTIIGHSTKPGFLERSSS 180

Query: 181 KISKVGGKPQQPIHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKTKN 240
           KISKVGGKPQ PI SLK+S+S+VKKSL+RESSN A        ASASASASAEIVPKTKN
Sbjct: 181 KISKVGGKPQPPIQSLKTSSSHVKKSLRRESSNVA--------ASASASASAEIVPKTKN 240

Query: 241 AVEHVDHSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEKKAVLDKDEADT 300
           AVEHVD SPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+RIDQVEKKA LDKDE DT
Sbjct: 241 AVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQVRIDQVEKKAALDKDETDT 300

Query: 301 ETFKVVVANKEVE--EIPEAAETEISLTNRERDEVE----GEEEKPERIAVEKEEVVGSE 360
           E  KVVV NKEVE  E P  AETEISL NRE++E E     EE+KP+RI VEKEEV+  E
Sbjct: 301 EILKVVVTNKEVEEKETPGTAETEISLANREQNEGEEEEPKEEDKPKRIGVEKEEVLALE 360

Query: 361 EINTNNKNDQEKSETDPVLDGESYKEENDH----KAAETTEKEAEATEKAAAAVVAKPRQ 420
           E + NNKNDQEKSETD VLDGES KEEND     KAAETTEKEAE+TEK      AKPRQ
Sbjct: 361 E-SINNKNDQEKSETDSVLDGESCKEENDQNSTPKAAETTEKEAESTEK-----TAKPRQ 420

Query: 421 GGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
           G PGRKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 GAPGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 451

BLAST of Bhi04G000874 vs. ExPASy TrEMBL
Match: A0A6J1HE16 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462632 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 1.2e-141
Identity = 331/484 (68.39%), Postives = 371/484 (76.65%), Query Frame = 0

Query: 1   MATTKAKENSGLGKERRITAPSSVTIPT-HTPTKRTPKSSPRGLISSTDPKLSSSSPSPS 60
           MAT++AKE+ GLGKER+ITAPSSVTIP  +T TKRTPKSSPRG+  STD KLSS   SPS
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGI--STDQKLSSFPSSPS 60

Query: 61  SSSSSSTLSLSVLPEKHIPNYLKSTASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRRSF 120
           SSSSSST SLS+LP+KH+PNYLKSTASSRHDHNFKP+ +S  GPAPIPEGKPSLNRRRSF
Sbjct: 61  SSSSSSTHSLSILPQKHVPNYLKSTASSRHDHNFKPIKKS-GGPAPIPEGKPSLNRRRSF 120

Query: 121 DKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFGAKPSTTTIVNSTKPGFLDRSSSKISK 180
           DKPP    RLGKPFRSP PRNRAAH PVRSSSFGAKPS T+IV+STK GFL+RS SKI K
Sbjct: 121 DKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPS-TSIVSSTKTGFLERSGSKIYK 180

Query: 181 VGGKPQQPIHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKTKNAVEH 240
           VGGK QQ + +LKSS SNV+KSLKRESSNAA            +++SAE+VPKTKNAVEH
Sbjct: 181 VGGKTQQSVQTLKSS-SNVRKSLKRESSNAA------------SASSAEMVPKTKNAVEH 240

Query: 241 VDHSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQL-RIDQVEKKAVLDKDEADTETF 300
           V  SP  VLG NEEDLKKIECELD  LPDP PELDK+L   DQVEK+    +   +T+ F
Sbjct: 241 VGQSPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLHHHQPIQETKIF 300

Query: 301 KVVVANKEVEEIPEAAETEISLT------NRERDEVEG-----EEEKPERIAVEKEEVVG 360
           KVV +NK+VEE P AAET+ + +      NRE++E EG     EEEKPERI VEKEEV G
Sbjct: 301 KVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKPERIEVEKEEVFG 360

Query: 361 --SEEINTNNKNDQEKSETDPVL------------DGESYKEEN---DHKAAETTEKEAE 420
             + E +TNNKN++EKS T P+             DG+S KEEN     K  E TEK A 
Sbjct: 361 VVASEESTNNKNEEEKSHTVPIQEGVIVLEHKEAEDGQSSKEENHTSTEKETEATEKVAA 420

Query: 421 ATEKAAAAVVAKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYE 455
           A   AA AV AKPRQGGPGRKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTVIDYE
Sbjct: 421 AATSAAEAVAAKPRQGGPGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYE 467

BLAST of Bhi04G000874 vs. ExPASy TrEMBL
Match: A0A6J1K599 (microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492415 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 6.0e-141
Identity = 333/488 (68.24%), Postives = 377/488 (77.25%), Query Frame = 0

Query: 1   MATTKAKENSGLGKERRITAPSSVTIPT-HTPTKRTPKSSPRGLISSTDPKLSS--SSPS 60
           MAT++AKE+ GLGKER+IT PSSVTIP  +T TKRTPKSSPRG+  STD KLSS  SSPS
Sbjct: 1   MATSRAKESCGLGKERKITTPSSVTIPNPNTSTKRTPKSSPRGI--STDQKLSSFPSSPS 60

Query: 61  PSSSSSSSTLSLSVLPEKHIPNYLKSTASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRR 120
           P   SSSST SLS+LP+KH+PNYLKSTASSRHDHNFKP+ +S +GPAPIPEGKPSLNRRR
Sbjct: 61  P---SSSSTHSLSILPQKHVPNYLKSTASSRHDHNFKPIKKS-AGPAPIPEGKPSLNRRR 120

Query: 121 SFDKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFGAKPSTTTIVNSTKPGFLDRSSSKI 180
           SFDKPP    RLGKPFRSP PRNRAAH PVRSSSFGAKPS TT+V+STK GFL+RS SKI
Sbjct: 121 SFDKPPPAMQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPS-TTLVSSTKTGFLERSGSKI 180

Query: 181 SKVGGKPQQPIHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKTKNAV 240
            KVGGK QQ + + KSS SN++KSLKRESSNAA            +++SAE+VPKTKNAV
Sbjct: 181 YKVGGKTQQSVQTFKSS-SNLRKSLKRESSNAA------------SASSAEMVPKTKNAV 240

Query: 241 EHVDHSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQL-RIDQVEKKAVLDKDEADTE 300
           EHV  SP  VLGVNEEDLKKIECELD  LPDP PELDK+L   DQVEK+    +   DT+
Sbjct: 241 EHVGQSPVLVLGVNEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLHHHQPIQDTK 300

Query: 301 TFKVVVANKEVEEIPEAAETEISLT------NRERDEVEG-----EEEKPERIAVEKEEV 360
            FKVV +NK+VEE P AAET+ + +      N+E++E EG     EEEKPERI VEKEEV
Sbjct: 301 IFKVVGSNKKVEETPGAAETDNAKSSIWEDENQEQNEGEGEEEAKEEEKPERIEVEKEEV 360

Query: 361 VG--SEEINTNNKNDQEKSETDPVL------------DGESYKEENDHKAAETTEKEAEA 420
            G  + E +TNNKN++EKS TDP+             DG+S KEEN +    +TEKEAEA
Sbjct: 361 YGVVASEESTNNKNEEEKSHTDPIQEGVIVLEHKEAEDGQSSKEENHN----STEKEAEA 420

Query: 421 TEK-----AAAAVVAKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTV 455
           TEK     AAAA  AKPRQGGPGRKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTV
Sbjct: 421 TEKVAAAAAAAAEAAKPRQGGPGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTV 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G24880.14.4e-1130.32BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (... [more]
AT5G10660.13.2e-0930.58calmodulin-binding protein-related [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LS859.5e-17980.77CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000... [more]
A0A5A7VBK42.0e-17680.17Serine/arginine repetitive matrix protein 1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BBU42.9e-17579.74serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488... [more]
A0A6J1HE161.2e-14168.39neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462... [more]
A0A6J1K5996.0e-14168.24microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 329..448
e-value: 0.0014
score: 16.0
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 354..447
e-value: 7.6E-16
score: 58.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 187..229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..393
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..417
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..72
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..229
NoneNo IPR availablePANTHERPTHR33349:SF20CALMODULIN-BINDING PROTEIN-LIKE PROTEINcoord: 1..451
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 1..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M000874Bhi04M000874mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding