Bhi04G000629 (gene) Wax gourd (B227) v1

BLAST of Bhi04G000629 vs. TAIR 10
Match: AT1G34770.1 (CONTAINS InterPro DOMAIN/s: MAGE protein (InterPro:IPR002190); Has 1274 Blast hits to 1260 proteins in 85 species: Archae - 0; Bacteria - 0; Metazoa - 1104; Fungi - 45; Plants - 49; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink). )

HSP 1 Score: 288.5 bits (737), Expect = 4.7e-78
Identity = 141/225 (62.67%), Postives = 184/225 (81.78%), Query Frame = 0

Query: 1   MASAGEYLSQMDISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLP 60
           MA   + LSQ DIS+EE DKLV+E IR+I+FK HQSSG PIKRE+LTQ+VTKNYR R L 
Sbjct: 1   MADEEDSLSQFDISKEETDKLVSEVIRFILFKFHQSSGTPIKREDLTQIVTKNYRQRNLA 60

Query: 61  AVVIDEAKKKLSSIFGYELRELQRTRLSSTGQAHSSQ-QNVADAKSYVLISKLPADIYRR 120
             VI+EAKKKLS++FGY+L+ELQR R SSTGQ+   Q Q+  D+KSYVL+S+LP +++++
Sbjct: 61  THVINEAKKKLSNVFGYDLKELQRARSSSTGQSRLPQSQSSVDSKSYVLVSELPLEVFKK 120

Query: 121 YVEDVNTAHMTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAE 180
           +V D  T+ +TGFTFVV++IV LAGGKIPEE+LWHHL+RMGL E+DE++PV GN K   E
Sbjct: 121 HVVDETTSPVTGFTFVVLAIVQLAGGKIPEETLWHHLKRMGLHENDEHNPVFGNNKQTLE 180

Query: 181 LVVQQRYLQKVKVNAPEGNITFYELAERAIDGPVSEKIKEYVAQV 225
            +VQQR+LQK KV+ PEG+  FY+LAERA+D  VSEK+K+Y++Q+
Sbjct: 181 TLVQQRFLQKEKVSGPEGSTLFYDLAERALDPQVSEKVKDYISQI 225

BLAST of Bhi04G000629 vs. ExPASy Swiss-Prot
Match: P43361 (Melanoma-associated antigen 8 OS=Homo sapiens OX=9606 GN=MAGEA8 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-08
Identity = 54/211 (25.59%), Postives = 96/211 (45.50%), Query Frame = 0

Query: 16  EEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLPAVVIDEAKKKLSSIF 75
           E  D+ VAE +R+++ K       P+ + E+ + V KNY+N   P  +  +A + +  IF
Sbjct: 110 EALDEKVAELVRFLLRKYQIKE--PVTKAEMLESVIKNYKNH-FPD-IFSKASECMQVIF 169

Query: 76  GYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRYVEDVNTAHMTGFTFV 135
           G +++E+                      SY+L++ L    Y   + D  +   TG   +
Sbjct: 170 GIDVKEVDPA-----------------GHSYILVTCLGLS-YDGLLGDDQSTPKTGLLII 229

Query: 136 VVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAELVVQQRYLQKVKVNAP 195
           V+ ++ + G + PEE++W  L  MGL +  E+       K   +  VQ+ YL+  +  AP
Sbjct: 230 VLGMILMEGSRAPEEAIWEALSVMGLYDGREHSVYWKLRKLLTQEWVQENYLEYRQ--AP 289

Query: 196 EGNITFYEL--AERAIDGPVSEKIKEYVAQV 225
             +   YE     RA+      K+ E+V +V
Sbjct: 290 GSDPVRYEFLWGPRALAETSYVKVLEHVVRV 296

BLAST of Bhi04G000629 vs. ExPASy Swiss-Prot
Match: P43355 (Melanoma-associated antigen 1 OS=Homo sapiens OX=9606 GN=MAGEA1 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.2e-08
Identity = 52/207 (25.12%), Postives = 93/207 (44.93%), Query Frame = 0

Query: 20  KLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLPAVVIDEAKKKLSSIFGYEL 79
           K VA+ + +++ K       P+ + E+ + V KNY++      +  +A + L  +FG ++
Sbjct: 104 KKVADLVGFLLLKYRARE--PVTKAEMLESVIKNYKH--CFPEIFGKASESLQLVFGIDV 163

Query: 80  RELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRYVEDVNTAHMTGFTFVVVSI 139
           +E   T                   SYVL++ L    Y   + D      TGF  +V+ +
Sbjct: 164 KEADPT-----------------GHSYVLVTCLGLS-YDGLLGDNQIMPKTGFLIIVLVM 223

Query: 140 VHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAELVVQQRYLQKVKVNAPEGNI 199
           + + GG  PEE +W  L  M + +  E+       K   + +VQ++YL+  +V  P+ + 
Sbjct: 224 IAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQV--PDSDP 283

Query: 200 TFYEL--AERAIDGPVSEKIKEYVAQV 225
             YE     RA+      K+ EYV +V
Sbjct: 284 ARYEFLWGPRALAETSYVKVLEYVIKV 286

BLAST of Bhi04G000629 vs. ExPASy Swiss-Prot
Match: P43363 (Melanoma-associated antigen 10 OS=Homo sapiens OX=9606 GN=MAGEA10 PE=2 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 6.5e-08
Identity = 46/177 (25.99%), Postives = 83/177 (46.89%), Query Frame = 0

Query: 13  ISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLPAVVIDEAKKKLS 72
           +   E D+ V + +++++FK       PI + E+ + V +NY +     ++  EA + + 
Sbjct: 129 LPRSEIDEKVTDLVQFLLFKYQMKE--PITKAEILESVIRNYEDHF--PLLFSEASECML 188

Query: 73  SIFGYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRYVEDVNTAHMTGF 132
            +FG +++E+  T                   S+VL++ L    Y   + DV +   TG 
Sbjct: 189 LVFGIDVKEVDPT-----------------GHSFVLVTSLGL-TYDGMLSDVQSMPKTGI 248

Query: 133 TFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGN-IKHAAELVVQQRYLQ 189
             +++SIV + G   PEE +W  L  MGL +  E H + G   K   +  VQ+ YL+
Sbjct: 249 LILILSIVFIEGYCTPEEVIWEALNMMGLYDGME-HLIYGEPRKLLTQDWVQENYLE 282

BLAST of Bhi04G000629 vs. ExPASy TrEMBL
Match: A0A1S3BBA9 (uncharacterized protein LOC103488065 OS=Cucumis melo OX=3656 GN=LOC103488065 PE=4 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.0e-112
Identity = 210/224 (93.75%), Postives = 219/224 (97.77%), Query Frame = 0

Query: 1   MASAGEYLSQMDISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLP 60
           MASAGE+LSQ+DISEEEKDKLVAEAIRYIIFKTHQ+SGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MASAGEHLSQLDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AVVIDEAKKKLSSIFGYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRY 120
           A+VIDEAK+KLSSIFGYELRELQRTR SSTGQ HSSQQNVADAKSYVLISKLPADIYR+Y
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKY 120

Query: 121 VEDVNTAHMTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAEL 180
           VEDV+TAH+TGFTFVVVSIVHLAGGKIPEESL HHLRRMGLSESDENHPVLGNIKHA EL
Sbjct: 121 VEDVDTAHVTGFTFVVVSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERAIDGPVSEKIKEYVAQV 225
           VVQQRYLQKVKVNAPEGNITFYELAERA DGPVSEKIKEYVAQ+
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQI 224

BLAST of Bhi04G000629 vs. ExPASy TrEMBL
Match: A0A0A0LL83 (MAGE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368960 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.7e-110
Identity = 205/224 (91.52%), Postives = 216/224 (96.43%), Query Frame = 0

Query: 1   MASAGEYLSQMDISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLP 60
           MASAGEY  ++DISEEEKDKLVAEAIRYIIFKTHQ+SGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AVVIDEAKKKLSSIFGYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRY 120
           A+VIDEAK+KLSSIFGYELRELQRTR SSTGQ HSSQQNV DAKSYVLISKLPADIYR+Y
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120

Query: 121 VEDVNTAHMTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAEL 180
           VEDVNTAH+TGFTFV++SIVHLAGGKIPEESL HHL+RMGLSESDENHPVLGNIKHA EL
Sbjct: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERAIDGPVSEKIKEYVAQV 225
           VVQQRYLQKVKVNAPEGNITFYELAERA DGPVSEKIKEYVAQ+
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQI 222

BLAST of Bhi04G000629 vs. ExPASy TrEMBL
Match: A0A6J1BU01 (melanoma-associated antigen 1 OS=Momordica charantia OX=3673 GN=LOC111004771 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 8.8e-109
Identity = 199/224 (88.84%), Postives = 213/224 (95.09%), Query Frame = 0

Query: 1   MASAGEYLSQMDISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLP 60
           MA AG+YLSQ+DIS EEKDKLVAEAIRYIIFKTHQ+SGCPIKREELTQLVTKNYRNRGLP
Sbjct: 1   MAGAGDYLSQIDISNEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AVVIDEAKKKLSSIFGYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRY 120
           AVVI+EAK+K+SSIFGYELRELQRTR SST Q HSSQQNVAD KSYVLISKLPADIYR+Y
Sbjct: 61  AVVIEEAKEKMSSIFGYELRELQRTRPSSTSQGHSSQQNVADTKSYVLISKLPADIYRKY 120

Query: 121 VEDVNTAHMTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAEL 180
           VEDVNTAH+TGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDE HPVLGNIK A EL
Sbjct: 121 VEDVNTAHVTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERAIDGPVSEKIKEYVAQV 225
           +VQ+RYLQK K+N PEGNITFYELAERA+DGPVSE+IKEYVAQ+
Sbjct: 181 LVQKRYLQKAKINGPEGNITFYELAERALDGPVSEEIKEYVAQI 224

BLAST of Bhi04G000629 vs. ExPASy TrEMBL
Match: A0A5A7VDL8 (Melanoma-associated antigen 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004830 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 7.7e-105
Identity = 196/208 (94.23%), Postives = 203/208 (97.60%), Query Frame = 0

Query: 17  EKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLPAVVIDEAKKKLSSIFG 76
           EKDKLVAEAIRYIIFKTHQ+SGCPIKREELTQLVTKNYRNRGLPA+VIDEAK+KLSSIFG
Sbjct: 10  EKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLPAIVIDEAKRKLSSIFG 69

Query: 77  YELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRYVEDVNTAHMTGFTFVV 136
           YELRELQRTR SSTGQ HSSQQNVADAKSYVLISKLPADIYR+YVEDV+TAH+TGFTFVV
Sbjct: 70  YELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKYVEDVDTAHVTGFTFVV 129

Query: 137 VSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAELVVQQRYLQKVKVNAPE 196
           VSIVHLAGGKIPEESL HHLRRMGLSESDENHPVLGNIKHA ELVVQQRYLQKVKVNAPE
Sbjct: 130 VSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIELVVQQRYLQKVKVNAPE 189

Query: 197 GNITFYELAERAIDGPVSEKIKEYVAQV 225
           GNITFYELAERA DGPVSEKIKEYVAQ+
Sbjct: 190 GNITFYELAERATDGPVSEKIKEYVAQI 217

BLAST of Bhi04G000629 vs. ExPASy TrEMBL
Match: A0A6J1KPM1 (melanoma-associated antigen 1 OS=Cucurbita maxima OX=3661 GN=LOC111496460 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.0e-104
Identity = 190/224 (84.82%), Postives = 208/224 (92.86%), Query Frame = 0

Query: 1   MASAGEYLSQMDISEEEKDKLVAEAIRYIIFKTHQSSGCPIKREELTQLVTKNYRNRGLP 60
           MAS+G+YLSQ+DIS EEKDKLVAE IRYIIFKTHQ+SGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AVVIDEAKKKLSSIFGYELRELQRTRLSSTGQAHSSQQNVADAKSYVLISKLPADIYRRY 120
           A+VI+EAK+KLSSIFGYELRELQR R SST Q H  Q NV D+KSYVLISKLPA++YR+Y
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVNTAHMTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDENHPVLGNIKHAAEL 180
           VEDV+TAH+TGF FVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DE HPVLGNIK A EL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 VVQQRYLQKVKVNAPEGNITFYELAERAIDGPVSEKIKEYVAQV 225
           +VQQRYLQK K+N PEGNITFYELAERAIDGPVSEKIKEYVAQ+
Sbjct: 181 LVQQRYLQKAKINGPEGNITFYELAERAIDGPVSEKIKEYVAQI 224