Bhi04G000492 (gene) Wax gourd (B227) v1

Overview
NameBhi04G000492
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionYqaJ domain-containing protein
Locationchr4: 13694468 .. 13697169 (+)
RNA-Seq ExpressionBhi04G000492
SyntenyBhi04G000492
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGCCCAAGCCTGGTGTTCTCACGAGTCATAAGCATAGCTCATCGCCATCGACGTTCCCAAAGGCTCCCTCTTTGTTCATGACCTTTGGAGTTAATTCCATTGCACGAGCTGAATAAACCCTCAATCGTCATCCTATTGCCACCATTGACGCAATCAGCAGCTCGAACTTCAAGCTTCATTTCATGGTGCATCTCTCCTCTTTTCTATGAACTGCTAATGAAACAATGAAGCTCGCTGCGGTCTCTTTCTCTCGGGCTGGAGCGTCTCAAAGGCTTCTTCATGGAGGTTCCTCTTTCAATCGATTTCCGCGCGTTGCTTCATTTGCAGCTCGCCAAGTTGACGCATTCAGCTCAACTTCTCTTTTGGGTATTTCCTCAAACACCTTAGCCGCTACTTCTTTCCTGTCATTACAAGTATTTTGTTTCAATTTGGGGCTTAGGCTTGGATTCCCATTTCAATGTTCAGATGAATTCTCTTCTTATTCTTCTTCATCTTCGTGCTTTTAGATAATTATTATTATTTCTTCTTCTGGAGACAGGAAACAAGTTTTTCTGCTCGACATTGTTACTTATAGCGTCTTTGTGATAATCAATTTCCTTTTAATCTTCTATTTGTAAAAGATAATCTTGTTATCAACAGTTTGTTCCTTCCAATTTTATGCCTAAAATCATTCCCAAAGTTCTCAAAATATTGTAAAAACAGAAAATGCTAAGCGTAGAGTTCATTTCTTGTAAAGAGAGAACTGGAAGCTAAAGTTATTTCTGCCTCTGGACTATTATGGTTTTTAACTTCCAAGTTACCATTTCGCTAAGTCGAGCTGAATTGAAAATATTGTTCTTTGGTTGTTTCTGGGTATGTTTCTATGAATATGTGAGGCGTTGATTGCTTGATGCTACTACAGAGGTTCATGTGAATTGAGAAGCTATGGAAATCGAGCATCATTGCCCACAACGCATAAATGTGTTAGATGTATTCCCAAGAAAAAAACTGTAGGAAGATTGTCTTTATTCAGCTAACTTAGCACAGTTGTAATTTGCAAGTTGAAATAACTAGAAGAGGTGATCAACTTCCATTCCATGTCTTCTTGAGAATAAGATACAAATTCTTTGAATTCTATCCAGGATGGTCAAAGAAAAATACTTATCTTGGATTATCATTTGTGAATTGGATTTATAAGCCCTGCATTATACTTGGGAGGATTTCACACGCCTGGTTAGAAGTTGATTGCTGACAGCATCCAAACTTGAAGTTGGGACATTTCTTGAAATGTTGTTTCTAATCAAGTTACTATAGTAGAACTGTAGAATATGATTTACTTGCACACCAAGTCACTTGTGGCTAAAAAAAGGGATGAAATTCGTTATTTTCTTTAGAAGGTAACAAGCTCATCAATAACACTATTGTTATTTGCTACATCAATTGTTTGTACGATTTCTTTGAATGCTTTACCAGCAAAAACTGTCTAGGTCTATTTTGCACTTTTCACTTCGTCTACACATAATCTTTAGCTTAACTTTTAGCAGTCTGTGGGTTGTGCAGAACGCTCCATCATAGTAATTCTTCAGTCGAAACTGCCATCATGTCAACAATGAACAACACCTCCATTTCTAGAATTTGCTGTAGACATTCTAGAATGAATGCAAGACTGTTAAGACGGAAGCATGGAAGTGGTTCAAGAACCTTTTCAACATGTGCCTCGCCGTCTAGCTCCATAACCAACCCTCTAGTCATCCGTTTACCCTCAGCTTTGATTCTGGCTTCCCAGGTCACCCCATCGGATGCCCCTCAGCGTTCGGAAGAATGGTTTGCACTACGGAGAGACAGGCTGACAACAAGCACATTCAGCACAGCTTTAGGCTTCTGGAAAGGAAATCGTCGGTTTGAGCTATGGCACGAGAAAGTATTCCCTCCAGAGATTCAAAAACCAGAAGCACCACAGCAAAATGCCATGGAATGGGGTGTGCTCAATGAAGCAAATGCCATCGATCGGTATAAAAGCATCACGGGCCGAGATGTAAGCCTGTTGGGATTCGCAACTCACTCGGAACAGCAATTTGATTGGCTCGGGGCCTCCCCAGACGGCCTACTGGGATGCTTTCAAGGTGGTGGGATCCTGGAAGTAAAATGTCCATACAACAAGGGAAAGCCTGAGAAGGGACTGCCCTGGTCAACCATGCCTTTCTATTACATGCCACAGGTACAGGGTCAAATGGAGATAATGGACAGAGAATGGGCGGATTTATACTGCTGGACACCAAATGGAAGCACAATATTTCGGGTATGTAGGGAGCGTGGTTATTGGGATTTGATACGTGAAATGTTGAGGGAATTTTGGTGGGAAAATGTTGTTCCTGCAAGGGAGGCTTTGTTGTTGGGAAGAGAGGAACAGGTGAAATCATATAAGCCGACGTCCACTCACAAGCAGACTGGACTAGCAATTGCTAAGAGCATTAAATTGGCAAGCGAGGCCAAATTGTTGTGTAGGGAGATTGCTGGGCATATTGAATTTTACCGATGATTATTGGTTTCGATGTATTTGGCTGTTTTTGTCTCGGAATTACATTTCCCCACATATTTGTTCCATAATTTCCAGATTGATACTATTTGATTGACTATACAATATACATTTCATTTTGAAACTAAAAGAAAAGCCAGAGTAAAAAATCTATATTGGAATGTATCCCACTGGTTCAACTTTGCG

mRNA sequence

CGGCCCAAGCCTGGTGTTCTCACGAGTCATAAGCATAGCTCATCGCCATCGACGTTCCCAAAGGCTCCCTCTTTGTTCATGACCTTTGGAGTTAATTCCATTGCACGAGCTGAATAAACCCTCAATCGTCATCCTATTGCCACCATTGACGCAATCAGCAGCTCGAACTTCAAGCTTCATTTCATGGTGCATCTCTCCTCTTTTCTATGAACTGCTAATGAAACAATGAAGCTCGCTGCGGTCTCTTTCTCTCGGGCTGGAGCGTCTCAAAGGCTTCTTCATGGAGGTTCCTCTTTCAATCGATTTCCGCGCGTTGCTTCATTTGCAGCTCGCCAAGTTGACGCATTCAGCTCAACTTCTCTTTTGGTCTGTGGGTTGTGCAGAACGCTCCATCATAGTAATTCTTCAGTCGAAACTGCCATCATGTCAACAATGAACAACACCTCCATTTCTAGAATTTGCTGTAGACATTCTAGAATGAATGCAAGACTGTTAAGACGGAAGCATGGAAGTGGTTCAAGAACCTTTTCAACATGTGCCTCGCCGTCTAGCTCCATAACCAACCCTCTAGTCATCCGTTTACCCTCAGCTTTGATTCTGGCTTCCCAGGTCACCCCATCGGATGCCCCTCAGCGTTCGGAAGAATGGTTTGCACTACGGAGAGACAGGCTGACAACAAGCACATTCAGCACAGCTTTAGGCTTCTGGAAAGGAAATCGTCGGTTTGAGCTATGGCACGAGAAAGTATTCCCTCCAGAGATTCAAAAACCAGAAGCACCACAGCAAAATGCCATGGAATGGGGTGTGCTCAATGAAGCAAATGCCATCGATCGGTATAAAAGCATCACGGGCCGAGATGTAAGCCTGTTGGGATTCGCAACTCACTCGGAACAGCAATTTGATTGGCTCGGGGCCTCCCCAGACGGCCTACTGGGATGCTTTCAAGGTGGTGGGATCCTGGAAGTAAAATGTCCATACAACAAGGGAAAGCCTGAGAAGGGACTGCCCTGGTCAACCATGCCTTTCTATTACATGCCACAGGTACAGGGTCAAATGGAGATAATGGACAGAGAATGGGCGGATTTATACTGCTGGACACCAAATGGAAGCACAATATTTCGGGTATGTAGGGAGCGTGGTTATTGGGATTTGATACGTGAAATGTTGAGGGAATTTTGGTGGGAAAATGTTGTTCCTGCAAGGGAGGCTTTGTTGTTGGGAAGAGAGGAACAGGTGAAATCATATAAGCCGACGTCCACTCACAAGCAGACTGGACTAGCAATTGCTAAGAGCATTAAATTGGCAAGCGAGGCCAAATTGTTGTGTAGGGAGATTGCTGGGCATATTGAATTTTACCGATGATTATTGGTTTCGATGTATTTGGCTGTTTTTGTCTCGGAATTACATTTCCCCACATATTTGTTCCATAATTTCCAGATTGATACTATTTGATTGACTATACAATATACATTTCATTTTGAAACTAAAAGAAAAGCCAGAGTAAAAAATCTATATTGGAATGTATCCCACTGGTTCAACTTTGCG

Coding sequence (CDS)

ATGAAGCTCGCTGCGGTCTCTTTCTCTCGGGCTGGAGCGTCTCAAAGGCTTCTTCATGGAGGTTCCTCTTTCAATCGATTTCCGCGCGTTGCTTCATTTGCAGCTCGCCAAGTTGACGCATTCAGCTCAACTTCTCTTTTGGTCTGTGGGTTGTGCAGAACGCTCCATCATAGTAATTCTTCAGTCGAAACTGCCATCATGTCAACAATGAACAACACCTCCATTTCTAGAATTTGCTGTAGACATTCTAGAATGAATGCAAGACTGTTAAGACGGAAGCATGGAAGTGGTTCAAGAACCTTTTCAACATGTGCCTCGCCGTCTAGCTCCATAACCAACCCTCTAGTCATCCGTTTACCCTCAGCTTTGATTCTGGCTTCCCAGGTCACCCCATCGGATGCCCCTCAGCGTTCGGAAGAATGGTTTGCACTACGGAGAGACAGGCTGACAACAAGCACATTCAGCACAGCTTTAGGCTTCTGGAAAGGAAATCGTCGGTTTGAGCTATGGCACGAGAAAGTATTCCCTCCAGAGATTCAAAAACCAGAAGCACCACAGCAAAATGCCATGGAATGGGGTGTGCTCAATGAAGCAAATGCCATCGATCGGTATAAAAGCATCACGGGCCGAGATGTAAGCCTGTTGGGATTCGCAACTCACTCGGAACAGCAATTTGATTGGCTCGGGGCCTCCCCAGACGGCCTACTGGGATGCTTTCAAGGTGGTGGGATCCTGGAAGTAAAATGTCCATACAACAAGGGAAAGCCTGAGAAGGGACTGCCCTGGTCAACCATGCCTTTCTATTACATGCCACAGGTACAGGGTCAAATGGAGATAATGGACAGAGAATGGGCGGATTTATACTGCTGGACACCAAATGGAAGCACAATATTTCGGGTATGTAGGGAGCGTGGTTATTGGGATTTGATACGTGAAATGTTGAGGGAATTTTGGTGGGAAAATGTTGTTCCTGCAAGGGAGGCTTTGTTGTTGGGAAGAGAGGAACAGGTGAAATCATATAAGCCGACGTCCACTCACAAGCAGACTGGACTAGCAATTGCTAAGAGCATTAAATTGGCAAGCGAGGCCAAATTGTTGTGTAGGGAGATTGCTGGGCATATTGAATTTTACCGATGA

Protein sequence

MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNSSVETAIMSTMNNTSISRICCRHSRMNARLLRRKHGSGSRTFSTCASPSSSITNPLVIRLPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEIQKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFRVCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKLASEAKLLCREIAGHIEFYR
Homology
BLAST of Bhi04G000492 vs. TAIR 10
Match: AT1G67660.3 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 2.7e-107
Identity = 192/335 (57.31%), Postives = 245/335 (73.13%), Query Frame = 0

Query: 44  TSLLVCGLCRTLHHSNSSVETAIMSTMNNTSISRICCRHSRMNARLLRRKHGSGSRTFST 103
           T + VC +CR L  +  ++ + I+S M   SIS       + +  +  RK  S S   S 
Sbjct: 10  TFVTVC-VCRVLKPNKVALNSMILSAMRTCSISGFHTHLPKSSGSVSSRKRFS-STALSL 69

Query: 104 CASPSSSITNPLVIRLPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKG 163
                S   +P      S++I++S ++PSD PQ+SEEWFALR+D+LTTSTFSTALGFWKG
Sbjct: 70  ITQTISPFAHP-----RSSVIVSSLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKG 129

Query: 164 NRRFELWHEKVFPPEIQKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQ 223
           NRR ELWHEKV+  + +  E   + AM WGV  E++AI+RYK I G +V  +GFA HS +
Sbjct: 130 NRRAELWHEKVYDSDARVVEESARFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIHSNE 189

Query: 224 QFDWLGASPDGLLGCFQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDRE 283
           +F WLGASPDG+L CF   GILEVKCPYNKGK E  LPW  +P+YYMPQ+QGQMEIMDRE
Sbjct: 190 EFHWLGASPDGILDCF---GILEVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDRE 249

Query: 284 WADLYCWTPNGSTIFRVCRERGYWDLIREMLREFWWENVVPAREALLLGRE-EQVKSYKP 343
           W +LYCWT NGST+FRV R+R YW +I ++LREFWWE+V+PAREALLLG+E E+VK Y+P
Sbjct: 250 WVNLYCWTRNGSTVFRVMRDRSYWRIIHDVLREFWWESVIPAREALLLGKEDEEVKKYEP 309

Query: 344 TSTHKQTGLAIAKSIKLASEAKLLCREIAGHIEFY 378
           TSTHK+T LAIAKS+ LA+E+KL+CREIA H+EF+
Sbjct: 310 TSTHKRTKLAIAKSLNLAAESKLVCREIADHVEFF 334

BLAST of Bhi04G000492 vs. TAIR 10
Match: AT1G67660.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 385.2 bits (988), Expect = 6.1e-107
Identity = 191/331 (57.70%), Postives = 243/331 (73.41%), Query Frame = 0

Query: 48  VCGLCRTLHHSNSSVETAIMSTMNNTSISRICCRHSRMNARLLRRKHGSGSRTFSTCASP 107
           VC +CR L  +  ++ + I+S M   SIS       + +  +  RK  S S   S     
Sbjct: 35  VC-VCRVLKPNKVALNSMILSAMRTCSISGFHTHLPKSSGSVSSRKRFS-STALSLITQT 94

Query: 108 SSSITNPLVIRLPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRF 167
            S   +P      S++I++S ++PSD PQ+SEEWFALR+D+LTTSTFSTALGFWKGNRR 
Sbjct: 95  ISPFAHP-----RSSVIVSSLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKGNRRA 154

Query: 168 ELWHEKVFPPEIQKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDW 227
           ELWHEKV+  + +  E   + AM WGV  E++AI+RYK I G +V  +GFA HS ++F W
Sbjct: 155 ELWHEKVYDSDARVVEESARFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIHSNEEFHW 214

Query: 228 LGASPDGLLGCFQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADL 287
           LGASPDG+L CF   GILEVKCPYNKGK E  LPW  +P+YYMPQ+QGQMEIMDREW +L
Sbjct: 215 LGASPDGILDCF---GILEVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDREWVNL 274

Query: 288 YCWTPNGSTIFRVCRERGYWDLIREMLREFWWENVVPAREALLLGRE-EQVKSYKPTSTH 347
           YCWT NGST+FRV R+R YW +I ++LREFWWE+V+PAREALLLG+E E+VK Y+PTSTH
Sbjct: 275 YCWTRNGSTVFRVMRDRSYWRIIHDVLREFWWESVIPAREALLLGKEDEEVKKYEPTSTH 334

Query: 348 KQTGLAIAKSIKLASEAKLLCREIAGHIEFY 378
           K+T LAIAKS+ LA+E+KL+CREIA H+EF+
Sbjct: 335 KRTKLAIAKSLNLAAESKLVCREIADHVEFF 355

BLAST of Bhi04G000492 vs. TAIR 10
Match: AT1G67660.2 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 3.4e-105
Identity = 186/313 (59.42%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 66  IMSTMNNTSISRICCRHSRMNARLLRRKHGSGSRTFSTCASPSSSITNPLVIRLPSALIL 125
           I+S M   SIS       + +  +  RK  S S   S      S   +P      S++I+
Sbjct: 2   ILSAMRTCSISGFHTHLPKSSGSVSSRKRFS-STALSLITQTISPFAHP-----RSSVIV 61

Query: 126 ASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEIQKPEAP 185
           +S ++PSD PQ+SEEWFALR+D+LTTSTFSTALGFWKGNRR ELWHEKV+  + +  E  
Sbjct: 62  SSLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKGNRRAELWHEKVYDSDARVVEES 121

Query: 186 QQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQGGGIL 245
            + AM WGV  E++AI+RYK I G +V  +GFA HS ++F WLGASPDG+L CF   GIL
Sbjct: 122 ARFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIHSNEEFHWLGASPDGILDCF---GIL 181

Query: 246 EVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFRVCRERG 305
           EVKCPYNKGK E  LPW  +P+YYMPQ+QGQMEIMDREW +LYCWT NGST+FRV R+R 
Sbjct: 182 EVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDREWVNLYCWTRNGSTVFRVMRDRS 241

Query: 306 YWDLIREMLREFWWENVVPAREALLLGRE-EQVKSYKPTSTHKQTGLAIAKSIKLASEAK 365
           YW +I ++LREFWWE+V+PAREALLLG+E E+VK Y+PTSTHK+T LAIAKS+ LA+E+K
Sbjct: 242 YWRIIHDVLREFWWESVIPAREALLLGKEDEEVKKYEPTSTHKRTKLAIAKSLNLAAESK 301

Query: 366 LLCREIAGHIEFY 378
           L+CREIA H+EF+
Sbjct: 302 LVCREIADHVEFF 305

BLAST of Bhi04G000492 vs. TAIR 10
Match: AT1G13810.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 173.3 bits (438), Expect = 3.6e-43
Identity = 89/247 (36.03%), Postives = 143/247 (57.89%), Query Frame = 0

Query: 139 EEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEIQKPEAPQQNAMEWGVLNEA 198
           + W  LR++RLT S F+ A+GF    RR  LW EK+      KP A  + A  W + NE 
Sbjct: 60  KNWEDLRKNRLTASNFARAIGFSPDGRR-NLWLEKI---GAAKPFAGNR-ATFWDIENEV 119

Query: 199 NAIDRYKSITGRDVSLLGFATH---SEQQFDWLGASPDGLLGCFQGG----GILEVKCPY 258
            A++RY  +TG ++ +  F  +      + +WLGASPDG++   + G    G+LEVKCP+
Sbjct: 120 EALERYNELTGNEILIPEFVVYKNGESPEENWLGASPDGVINVVKDGVTSCGVLEVKCPF 179

Query: 259 NKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFRVCRERGYWDLIR 318
           +     K  PW  +P+  +PQ+QG MEI+D +W DLYCWT NGS++FRV R+  +W+ ++
Sbjct: 180 DNRDNSKVYPWKKVPYNCVPQLQGLMEIVDTDWLDLYCWTRNGSSLFRVWRDTAFWEDMK 239

Query: 319 EMLREFWWENVVPAREAL----LLGREEQVKSYKPTSTHKQTGLAIAKSIKLASEAKLLC 375
             L +FW  +V+PARE      +   + +++ +KP   H+     +  + ++++ A  L 
Sbjct: 240 PALFDFWQNHVLPAREIYNNFDIKDPQVKLREFKPKHWHEDCKKIMRGAERISANANRLF 299

BLAST of Bhi04G000492 vs. NCBI nr
Match: XP_038884042.1 (uncharacterized protein LOC120074988 isoform X1 [Benincasa hispida])

HSP 1 Score: 773.9 bits (1997), Expect = 6.6e-220
Identity = 378/378 (100.00%), Postives = 378/378 (100.00%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS
Sbjct: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARLLRRKHGSGSRTFSTCASPSSSITNPLVIRLP 120
           SVETAIMSTMNNTSISRICCRHSRMNARLLRRKHGSGSRTFSTCASPSSSITNPLVIRLP
Sbjct: 61  SVETAIMSTMNNTSISRICCRHSRMNARLLRRKHGSGSRTFSTCASPSSSITNPLVIRLP 120

Query: 121 SALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEIQ 180
           SALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEIQ
Sbjct: 121 SALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEIQ 180

Query: 181 KPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQ 240
           KPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQ
Sbjct: 181 KPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQ 240

Query: 241 GGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFRV 300
           GGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFRV
Sbjct: 241 GGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFRV 300

Query: 301 CRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKLA 360
           CRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKLA
Sbjct: 301 CRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKLA 360

Query: 361 SEAKLLCREIAGHIEFYR 379
           SEAKLLCREIAGHIEFYR
Sbjct: 361 SEAKLLCREIAGHIEFYR 378

BLAST of Bhi04G000492 vs. NCBI nr
Match: XP_022131520.1 (uncharacterized protein LOC111004694 isoform X1 [Momordica charantia])

HSP 1 Score: 675.2 bits (1741), Expect = 3.2e-190
Identity = 332/379 (87.60%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFS++GAS+  LHGG SFNR PRVAS +A +VDAF STSLLVCG CRTLH  NS
Sbjct: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLVCGFCRTLHQRNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARL-LRRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           S+ T  MSTM+ TSISRICCRH  +NARL  +RKHGSGSRTFSTC S SSS TNPLV R 
Sbjct: 61  SINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVTRF 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFP EI
Sbjct: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           QK EA Q+ AMEWGVLNEA AIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF
Sbjct: 181 QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKG+PEKGLPWSTMPFYYMPQVQGQMEIMDREW DLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGRPEKGLPWSTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYW+L+REMLREFWWENVVPAREAL LGREE+VKSYKPTSTHKQTGLAIAKSIKL
Sbjct: 301 VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKQTGLAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           ASEAKL+ REIAGH+EFYR
Sbjct: 361 ASEAKLMFREIAGHVEFYR 378

BLAST of Bhi04G000492 vs. NCBI nr
Match: XP_023537251.1 (uncharacterized protein LOC111798383 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 668.3 bits (1723), Expect = 3.9e-188
Identity = 323/379 (85.22%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFS++GAS+  LH  SSFNR PRVASF+ARQVDAFSSTSL VCG CRT H SNS
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPRVASFSARQVDAFSSTSLSVCGFCRTPHQSNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARLL-RRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           S++TA++STM+NTSI+RICCRH R NARL  +RK  +GSRTFST  SP  S+TNPL+IRL
Sbjct: 61  SIDTALLSTMSNTSIARICCRHPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIRL 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP EI
Sbjct: 121 PSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           +KPEA QQ AMEWGVLNE NAI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGCF
Sbjct: 181 KKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQ+EIMDREWADLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYW+LI EMLREFWWENVVPAREA   GRE++V+SYKPTSTHK TG+AIAKSIKL
Sbjct: 301 VCRERGYWELIHEMLREFWWENVVPAREASSSGREKEVESYKPTSTHKLTGVAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           AS+AKLLCREIAGH+EFYR
Sbjct: 361 ASDAKLLCREIAGHVEFYR 379

BLAST of Bhi04G000492 vs. NCBI nr
Match: KAG7020121.1 (hypothetical protein SDJN02_16803 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 665.2 bits (1715), Expect = 3.3e-187
Identity = 323/379 (85.22%), Postives = 347/379 (91.56%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFS++GAS+  LH  SSFNR P VASF+ARQVDAFSSTSL VCG CRT H SNS
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPCVASFSARQVDAFSSTSLSVCGFCRTPHQSNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARLL-RRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           S+ TA++STM+NTSI+RICCR  R NARL  +RK  +GSRTFST  SP  S+TNPL+IRL
Sbjct: 61  SINTALLSTMSNTSIARICCRLPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIRL 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP EI
Sbjct: 121 PSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           +KPEA QQ AMEWGVLNE NAI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGCF
Sbjct: 181 KKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQ+EIMDREWADLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYW+LI EMLREFWWENVVPAREAL  GRE++V+SYKPTSTHKQTG+AIAKSIKL
Sbjct: 301 VCRERGYWELIHEMLREFWWENVVPAREALSSGREKEVESYKPTSTHKQTGVAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           AS+AKLLCREIAGH+EFYR
Sbjct: 361 ASDAKLLCREIAGHVEFYR 379

BLAST of Bhi04G000492 vs. NCBI nr
Match: XP_022951164.1 (uncharacterized protein LOC111454092 isoform X1 [Cucurbita moschata])

HSP 1 Score: 662.9 bits (1709), Expect = 1.6e-186
Identity = 322/379 (84.96%), Postives = 346/379 (91.29%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFS++GAS+  LH  SSFNR P VASF+ARQVDAFSSTS  VCG CRT H SNS
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPCVASFSARQVDAFSSTSRSVCGFCRTPHQSNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARLL-RRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           S+ TA++STM+NTSI+RICCR  R NARL  +RK  +GSRTFST  SP  S+TNPL+IRL
Sbjct: 61  SINTALLSTMSNTSIARICCRLPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIRL 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP EI
Sbjct: 121 PSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           +KPEA QQ AMEWGVLNE NAI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGCF
Sbjct: 181 KKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQ+EIMDREWADLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYW+LI EMLREFWWENVVPAREAL  GRE++V+SYKPTSTHKQTG+AIAKSIKL
Sbjct: 301 VCRERGYWELIHEMLREFWWENVVPAREALSSGREKEVESYKPTSTHKQTGVAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           AS+AKLLCREIAGH+EFYR
Sbjct: 361 ASDAKLLCREIAGHVEFYR 379

BLAST of Bhi04G000492 vs. ExPASy TrEMBL
Match: A0A6J1BPX6 (uncharacterized protein LOC111004694 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004694 PE=4 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.5e-190
Identity = 332/379 (87.60%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFS++GAS+  LHGG SFNR PRVAS +A +VDAF STSLLVCG CRTLH  NS
Sbjct: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLVCGFCRTLHQRNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARL-LRRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           S+ T  MSTM+ TSISRICCRH  +NARL  +RKHGSGSRTFSTC S SSS TNPLV R 
Sbjct: 61  SINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVTRF 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFP EI
Sbjct: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           QK EA Q+ AMEWGVLNEA AIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF
Sbjct: 181 QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKG+PEKGLPWSTMPFYYMPQVQGQMEIMDREW DLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGRPEKGLPWSTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYW+L+REMLREFWWENVVPAREAL LGREE+VKSYKPTSTHKQTGLAIAKSIKL
Sbjct: 301 VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKQTGLAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           ASEAKL+ REIAGH+EFYR
Sbjct: 361 ASEAKLMFREIAGHVEFYR 378

BLAST of Bhi04G000492 vs. ExPASy TrEMBL
Match: A0A6J1GHX8 (uncharacterized protein LOC111454092 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454092 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.9e-187
Identity = 322/379 (84.96%), Postives = 346/379 (91.29%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFS++GAS+  LH  SSFNR P VASF+ARQVDAFSSTS  VCG CRT H SNS
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPCVASFSARQVDAFSSTSRSVCGFCRTPHQSNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARLL-RRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           S+ TA++STM+NTSI+RICCR  R NARL  +RK  +GSRTFST  SP  S+TNPL+IRL
Sbjct: 61  SINTALLSTMSNTSIARICCRLPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIRL 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP EI
Sbjct: 121 PSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           +KPEA QQ AMEWGVLNE NAI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGCF
Sbjct: 181 KKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQ+EIMDREWADLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYW+LI EMLREFWWENVVPAREAL  GRE++V+SYKPTSTHKQTG+AIAKSIKL
Sbjct: 301 VCRERGYWELIHEMLREFWWENVVPAREALSSGREKEVESYKPTSTHKQTGVAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           AS+AKLLCREIAGH+EFYR
Sbjct: 361 ASDAKLLCREIAGHVEFYR 379

BLAST of Bhi04G000492 vs. ExPASy TrEMBL
Match: A0A6J1KS64 (uncharacterized protein LOC111496007 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496007 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.1e-185
Identity = 319/379 (84.17%), Postives = 346/379 (91.29%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MKLAAVSFS++ AS+  LH  SSFNR PRVASF+  QVDAFSSTSL VC  CRT H SNS
Sbjct: 1   MKLAAVSFSQSVASRSFLHADSSFNRLPRVASFSPPQVDAFSSTSLSVCWFCRTPHQSNS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARLL-RRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           S++TA++STM+NTSI+RICC H R NARL  +RK  +GSRTFST  SP  S+TNPL+IRL
Sbjct: 61  SIDTALLSTMSNTSIARICCTHPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIRL 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKG+RRFELWHEKVFP EI
Sbjct: 121 PSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGSRRFELWHEKVFPSEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           +KPEA QQ AMEWGVLNE NAI RYKSITGRDVS LGFATHSEQQ +WLGASPDGLLGCF
Sbjct: 181 KKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLEWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQ+EIMDREWADLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYW+LI EMLREFWWENVVPAREAL LGREE+V+SYKPTSTHKQTG+AI+KSIKL
Sbjct: 301 VCRERGYWELIHEMLREFWWENVVPAREALSLGREEEVESYKPTSTHKQTGVAISKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           AS+AKLLCREIAGH+EFYR
Sbjct: 361 ASDAKLLCREIAGHVEFYR 379

BLAST of Bhi04G000492 vs. ExPASy TrEMBL
Match: A0A0A0LNG4 (YqaJ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G350400 PE=4 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.5e-185
Identity = 323/379 (85.22%), Postives = 340/379 (89.71%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MK AAVSFS++GAS+ LLHGGSSFN+   VAS +ARQ  +F+S SLLVCGLCRTL  S+S
Sbjct: 1   MKFAAVSFSQSGASRSLLHGGSSFNQLLPVASISARQFGSFNSNSLLVCGLCRTLRQSSS 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARL-LRRKHGSGSRTFSTCASPSSSITNPLVIRL 120
            VETAIMSTMNN SI+RICCRHSR NARL L+R H   SR FSTC SPSSS  NPLVI L
Sbjct: 61  LVETAIMSTMNNISIARICCRHSRKNARLYLKRNHEIASRPFSTCVSPSSSTKNPLVIWL 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PS L+LASQ   S APQRSEEWFALRRD+LTTSTFSTALGFWKGNRR ELWHEKVFP EI
Sbjct: 121 PSPLVLASQANQSVAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRIELWHEKVFPSEI 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           QK EAPQQNAMEWGVLNE NAIDRYK ITGRDVSLLGFATHSEQQFDWLGASPDGLL CF
Sbjct: 181 QKTEAPQQNAMEWGVLNEVNAIDRYKGITGRDVSLLGFATHSEQQFDWLGASPDGLLECF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGKPEKGLPWST+PFYYMPQVQGQMEIM REWADLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGKPEKGLPWSTIPFYYMPQVQGQMEIMGREWADLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYWDLIRE+LREFWWENVVPA+EALLLG EE+ KSYKPTSTHKQTGLAIAKSIKL
Sbjct: 301 VCRERGYWDLIREILREFWWENVVPAKEALLLGSEEKAKSYKPTSTHKQTGLAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           ASEAKL CREIAGH+EFYR
Sbjct: 361 ASEAKLFCREIAGHVEFYR 379

BLAST of Bhi04G000492 vs. ExPASy TrEMBL
Match: A0A1S3B9S9 (uncharacterized protein LOC103487752 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487752 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.4e-183
Identity = 324/379 (85.49%), Postives = 343/379 (90.50%), Query Frame = 0

Query: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLLVCGLCRTLHHSNS 60
           MK AAVSFS++GAS+ L HGGSSFN+ P VASF+AR+    +S SLLVCGLCRTL  SN 
Sbjct: 1   MKFAAVSFSQSGASRSLFHGGSSFNQLPPVASFSARKF-PLNSDSLLVCGLCRTLCQSN- 60

Query: 61  SVETAIMSTMNNTSISRICCRHSRMNARL-LRRKHGSGSRTFSTCASPSSSITNPLVIRL 120
           SVE AIMSTMNN SI+RICCR SR NA+L L+R  G  SR+FSTCA+PSS  TNP VI L
Sbjct: 61  SVEIAIMSTMNNISIARICCRDSRKNAKLYLKRNRGIASRSFSTCATPSSYTTNPPVIWL 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPEI 180
           PS LILASQV  S APQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP E 
Sbjct: 121 PSPLILASQVNQSVAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSET 180

Query: 181 QKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           QK +APQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF
Sbjct: 181 QKTDAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIM REW+DLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMGREWSDLYCWTPNGSTIFR 300

Query: 301 VCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIKL 360
           VCRERGYWDLIRE+L+EFWWENVVPA+EAL LGREEQ KSYKPTSTHKQTGLAIAKSIKL
Sbjct: 301 VCRERGYWDLIREILKEFWWENVVPAKEALSLGREEQAKSYKPTSTHKQTGLAIAKSIKL 360

Query: 361 ASEAKLLCREIAGHIEFYR 379
           ASEAKLLCREIAGH+EFYR
Sbjct: 361 ASEAKLLCREIAGHVEFYR 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G67660.32.7e-10757.31Restriction endonuclease, type II-like superfamily protein [more]
AT1G67660.16.1e-10757.70Restriction endonuclease, type II-like superfamily protein [more]
AT1G67660.23.4e-10559.42Restriction endonuclease, type II-like superfamily protein [more]
AT1G13810.13.6e-4336.03Restriction endonuclease, type II-like superfamily protein [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_038884042.16.6e-220100.00uncharacterized protein LOC120074988 isoform X1 [Benincasa hispida][more]
XP_022131520.13.2e-19087.60uncharacterized protein LOC111004694 isoform X1 [Momordica charantia][more]
XP_023537251.13.9e-18885.22uncharacterized protein LOC111798383 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7020121.13.3e-18785.22hypothetical protein SDJN02_16803 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022951164.11.6e-18684.96uncharacterized protein LOC111454092 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1BPX61.5e-19087.60uncharacterized protein LOC111004694 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1GHX87.9e-18784.96uncharacterized protein LOC111454092 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KS641.1e-18584.17uncharacterized protein LOC111496007 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0LNG42.5e-18585.22YqaJ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G350400 PE=4 S... [more]
A0A1S3B9S92.4e-18385.49uncharacterized protein LOC103487752 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019080YqaJ viral recombinasePFAMPF09588YqaJcoord: 140..282
e-value: 2.7E-19
score: 69.8
IPR011604Exonuclease, phage-type/RecB, C-terminalGENE3D3.90.320.10coord: 135..329
e-value: 1.0E-49
score: 171.0
IPR017482Putative phage-type endonucleaseTIGRFAMTIGR03033TIGR03033coord: 135..292
e-value: 1.4E-21
score: 75.1
NoneNo IPR availablePANTHERPTHR46609:SF6RESTRICTION ENDONUCLEASE, TYPE II-LIKE SUPERFAMILY PROTEINcoord: 67..377
NoneNo IPR availablePANTHERPTHR46609EXONUCLEASE, PHAGE-TYPE/RECB, C-TERMINAL DOMAIN-CONTAINING PROTEINcoord: 67..377
IPR011335Restriction endonuclease type II-likeSUPERFAMILY52980Restriction endonuclease-likecoord: 132..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M000492Bhi04M000492mRNA