Bhi04G000351 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G000351
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	SASA domain-containing protein
Location	chr4: 9502637 .. 9503099 (-)
RNA-Seq Expression	Bhi04G000351
Synteny	Bhi04G000351

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAGCCGAGGTGGAGTTGAGCATAATCAAGTGGGAAAGCTTGAGTGGGACAGGTAGTCCCACCAGAATGTTAATTTGACCCATCCATCCTACGATTGAACCTTGTGCACCAATAGGAGATAGTACGAGAACCTCTCCATGAGGCGATCGACATCAACAAAACGGTTGGGATTGGTCTAGAAATGGCATTTGCTCACCAACTATTAGCAAAGTAGGGCCAAAAGCAAGCATAGTGGATTTAGTTCCTTGTGCTAGAGGTGGCACCATAATTGAACAATGGATTAACAATCCTAACAACCCTGATGCAACATTTTACAAAAATTTCATCAAACGAATCAAAGTATCAGATAAAGAAGGTGGGGTTGTGCGTGCTCTTTTCTGGTTTCAAGGGGAGAGTGATGCAGCTATGAGTGATACTGCTAGTAGATACAAAGACAACCTAAACCGACATCTGCAATGA

mRNA sequence

ATGGCTAGCCGAGGTGGAGTTGAGCATAATCAAGTGGGAAAGCTTGAGTGGGACAGAAATGGCATTTGCTCACCAACTATTAGCAAAGTAGGGCCAAAAGCAAGCATAGTGGATTTAGTTCCTTGTGCTAGAGGTGGCACCATAATTGAACAATGGATTAACAATCCTAACAACCCTGATGCAACATTTTACAAAAATTTCATCAAACGAATCAAAGTATCAGATAAAGAAGGTGGGGTTGTGCGTGCTCTTTTCTGGTTTCAAGGGGAGAGTGATGCAGCTATGAGTGATACTGCTAGTAGATACAAAGACAACCTAAACCGACATCTGCAATGA

Coding sequence (CDS)

ATGGCTAGCCGAGGTGGAGTTGAGCATAATCAAGTGGGAAAGCTTGAGTGGGACAGAAATGGCATTTGCTCACCAACTATTAGCAAAGTAGGGCCAAAAGCAAGCATAGTGGATTTAGTTCCTTGTGCTAGAGGTGGCACCATAATTGAACAATGGATTAACAATCCTAACAACCCTGATGCAACATTTTACAAAAATTTCATCAAACGAATCAAAGTATCAGATAAAGAAGGTGGGGTTGTGCGTGCTCTTTTCTGGTTTCAAGGGGAGAGTGATGCAGCTATGAGTGATACTGCTAGTAGATACAAAGACAACCTAAACCGACATCTGCAATGA

Protein sequence

MASRGGVEHNQVGKLEWDRNGICSPTISKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKEGGVVRALFWFQGESDAAMSDTASRYKDNLNRHLQ

Homology

[Close]

BLAST of Bhi04G000351 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 62.8 bits (151), Expect = 2.0e-10
Identity = 28/75 (37.33%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 34  ASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKEGGVVRALFWFQGESDA 93
           ++++ LVPCA GGT I++W    +      Y+  +KR + S K GG ++A+ W+QGESD 
Sbjct: 110 SAVIGLVPCASGGTAIKEWERGSH-----LYERMVKRTEESRKCGGEIKAVLWYQGESDV 169

Query: 94  AMSDTASRYKDNLNR 109
                A  Y +N++R
Sbjct: 170 LDIHDAESYGNNMDR 179

[Close]

BLAST of Bhi04G000351 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 62.8 bits (151), Expect = 2.0e-10
Identity = 28/75 (37.33%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 34  ASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKEGGVVRALFWFQGESDA 93
           ++++ LVPCA GGT I++W    +      Y+  +KR + S K GG ++A+ W+QGESD 
Sbjct: 110 SAVIGLVPCASGGTAIKEWERGSH-----LYERMVKRTEESRKCGGEIKAVLWYQGESDV 169

Query: 94  AMSDTASRYKDNLNR 109
                A  Y +N++R
Sbjct: 170 LDIHDAESYGNNMDR 179

[Close]

BLAST of Bhi04G000351 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 53.1 bits (126), Expect = 1.6e-07
Identity = 31/84 (36.90%), Postives = 42/84 (50.00%), Query Frame = 0


Query: 25  PTISKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIK--VSDKEGGVVR 84
           P  ++V  +   V LVPC+ GGT + QW           Y+  +KR K  ++   GG  R
Sbjct: 104 PFANRVVNRFGQVGLVPCSIGGTKLSQW-----QKGEFLYEETVKRAKAAMASGGGGSYR 163

Query: 85  ALFWFQGESDAAMSDTASRYKDNL 107
           A+ W+QGESD      AS YK  L
Sbjct: 164 AVLWYQGESDTVDMVDASVYKKRL 182

[Close]

BLAST of Bhi04G000351 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 2.9e-09
Identity = 28/75 (37.33%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 34  ASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKEGGVVRALFWFQGESDA 93
           ++++ LVPCA GGT I++W    +      Y+  +KR + S K GG ++A+ W+QGESD 
Sbjct: 110 SAVIGLVPCASGGTAIKEWERGSH-----LYERMVKRTEESRKCGGEIKAVLWYQGESDV 169

Query: 94  AMSDTASRYKDNLNR 109
                A  Y +N++R
Sbjct: 170 LDIHDAESYGNNMDR 179

[Close]

BLAST of Bhi04G000351 vs. NCBI nr
Match: XP_038886575.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 164.5 bits (415), Expect = 5.3e-37
Identity = 87/149 (58.39%), Postives = 96/149 (64.43%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDR-----------------------------NGI--------- 60
           MA RGGVE+NQVGKLEW+R                              GI         
Sbjct: 37  MAGRGGVENNQVGKLEWNRLIPPECQSDTSILRLNPALQWEMAREPLHEGIDINKTVGIG 96

Query: 61  -----CSPTISKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKE 107
                    ++KVGPKA IV LVPCA+GGTIIEQWI NP+NPDATFYK+FI+RIK SDKE
Sbjct: 97  PGMPFAHQLLAKVGPKAGIVGLVPCAKGGTIIEQWIKNPSNPDATFYKSFIERIKASDKE 156

[Close]

BLAST of Bhi04G000351 vs. NCBI nr
Match: KAA0066252.1 (putative carbohydrate esterase [Cucumis melo var. makuwa] >TYJ97669.1 putative carbohydrate esterase [Cucumis melo var. makuwa])

HSP 1 Score: 161.4 bits (407), Expect = 4.5e-36
Identity = 77/112 (68.75%), Postives = 89/112 (79.46%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDR--NGICSP----TISKVGPKASIVDLVPCARGGTIIEQWIN 60
           +A RGGVE +Q+G L WDR     C P     ++K GP A +V LVPCARGGT+IEQWI 
Sbjct: 37  IAGRGGVEKDQLGNLVWDRLVPPECMPFAHHLLAKAGPNACVVGLVPCARGGTLIEQWIK 96

Query: 61  NPNNPDATFYKNFIKRIKVSDKEGGVVRALFWFQGESDAAMSDTASRYKDNL 107
           NP+NP+ATFYK FI+RIK SDK+GGVVRALFWFQGE+DAAMSDTA RYKDNL
Sbjct: 97  NPSNPNATFYKKFIERIKASDKDGGVVRALFWFQGENDAAMSDTAHRYKDNL 148

[Close]

BLAST of Bhi04G000351 vs. NCBI nr
Match: XP_038886442.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 160.2 bits (404), Expect = 1.0e-35
Identity = 85/151 (56.29%), Postives = 94/151 (62.25%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDRNGICSP----------------------------------- 60
           MA RGGVE+NQV +LEWD  G+  P                                   
Sbjct: 37  MAGRGGVENNQVRELEWD--GLIPPECQSDPSILRLNPALQWEIAREPLHEGIDINKTVG 96

Query: 61  ----------TISKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSD 107
                      ++KVGP+A  V LVPCARGGTIIEQWI NP+NPDATFYKNFI+RIK SD
Sbjct: 97  IGPGMPFAHQLLTKVGPRAGTVGLVPCARGGTIIEQWIKNPSNPDATFYKNFIERIKASD 156

[Close]

BLAST of Bhi04G000351 vs. NCBI nr
Match: XP_023002177.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002871.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 152.1 bits (383), Expect = 2.7e-33
Identity = 83/153 (54.25%), Postives = 93/153 (60.78%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDRN-----------------------------GI-------CS 60
           MA RGGVE+NQ GKLEWD                               GI         
Sbjct: 37  MAGRGGVENNQKGKLEWDGKVPLECQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIG 96

Query: 61  PTI-------SKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKE 111
           P I       +K G KA IV LVPCARGGT+IEQWI NP+NP ATFY+NFI+RIK S+KE
Sbjct: 97  PGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 156

[Close]

BLAST of Bhi04G000351 vs. NCBI nr
Match: XP_023002892.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 152.1 bits (383), Expect = 2.7e-33
Identity = 83/153 (54.25%), Postives = 93/153 (60.78%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDRN-----------------------------GI-------CS 60
           MA RGGVE+NQ GKLEWD                               GI         
Sbjct: 1   MAGRGGVENNQKGKLEWDGKVPLECQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIG 60

Query: 61  PTI-------SKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKE 111
           P I       +K G KA IV LVPCARGGT+IEQWI NP+NP ATFY+NFI+RIK S+KE
Sbjct: 61  PGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 120

[Close]

BLAST of Bhi04G000351 vs. ExPASy TrEMBL
Match: A0A5D3BF43 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37G00380 PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.2e-36
Identity = 77/112 (68.75%), Postives = 89/112 (79.46%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDR--NGICSP----TISKVGPKASIVDLVPCARGGTIIEQWIN 60
           +A RGGVE +Q+G L WDR     C P     ++K GP A +V LVPCARGGT+IEQWI 
Sbjct: 37  IAGRGGVEKDQLGNLVWDRLVPPECMPFAHHLLAKAGPNACVVGLVPCARGGTLIEQWIK 96

Query: 61  NPNNPDATFYKNFIKRIKVSDKEGGVVRALFWFQGESDAAMSDTASRYKDNL 107
           NP+NP+ATFYK FI+RIK SDK+GGVVRALFWFQGE+DAAMSDTA RYKDNL
Sbjct: 97  NPSNPNATFYKKFIERIKASDKDGGVVRALFWFQGENDAAMSDTAHRYKDNL 148

[Close]

BLAST of Bhi04G000351 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.3e-33
Identity = 83/153 (54.25%), Postives = 93/153 (60.78%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDRN-----------------------------GI-------CS 60
           MA RGGVE+NQ GKLEWD                               GI         
Sbjct: 37  MAGRGGVENNQKGKLEWDGKVPLECQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIG 96

Query: 61  PTI-------SKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKE 111
           P I       +K G KA IV LVPCARGGT+IEQWI NP+NP ATFY+NFI+RIK S+KE
Sbjct: 97  PGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 156

[Close]

BLAST of Bhi04G000351 vs. ExPASy TrEMBL
Match: A0A6J1KKV2 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496632 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.3e-33
Identity = 83/153 (54.25%), Postives = 93/153 (60.78%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDRN-----------------------------GI-------CS 60
           MA RGGVE+NQ GKLEWD                               GI         
Sbjct: 1   MAGRGGVENNQKGKLEWDGKVPLECQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIG 60

Query: 61  PTI-------SKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKE 111
           P I       +K G KA IV LVPCARGGT+IEQWI NP+NP ATFY+NFI+RIK S+KE
Sbjct: 61  PGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 120

[Close]

BLAST of Bhi04G000351 vs. ExPASy TrEMBL
Match: A0A5A7VGP3 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37G00180 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.7e-33
Identity = 80/151 (52.98%), Postives = 90/151 (59.60%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDR--NGICSP--------------------------------- 60
           MA RGGVE +Q G L WDR     C P                                 
Sbjct: 43  MAGRGGVEKDQSGNLVWDRLVPPECEPQPSILRLNPEREWETAREPLHVGIDINRTAGIG 102

Query: 61  --------TISKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKE 109
                    ++K GP A +V LVPCARGGT+IEQWI NP+NP+ATFYKNFI+RIK SDK+
Sbjct: 103 PGMPFAHHLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFYKNFIERIKASDKD 162

[Close]

BLAST of Bhi04G000351 vs. ExPASy TrEMBL
Match: A0A5A7VGI5 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G003000 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.7e-33
Identity = 80/151 (52.98%), Postives = 90/151 (59.60%), Query Frame = 0


Query: 1   MASRGGVEHNQVGKLEWDR--NGICSP--------------------------------- 60
           MA RGGVE +Q G L WDR     C P                                 
Sbjct: 1   MAGRGGVEKDQSGNLVWDRLVPPECEPQPSILRLNPEREWETAREPLHVGIDINRTAGIG 60

Query: 61  --------TISKVGPKASIVDLVPCARGGTIIEQWINNPNNPDATFYKNFIKRIKVSDKE 109
                    ++K GP A +V LVPCARGGT+IEQWI NP+NP+ATFYKNFI+RIK SDK+
Sbjct: 61  PGMPFAHHLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFYKNFIERIKASDKD 120

The following BLAST results are available for this feature:

BLAST of Bhi04G000351 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34215.1	2.0e-10	37.33	Domain of unknown function (DUF303)	[more]
AT4G34215.2	2.0e-10	37.33	Domain of unknown function (DUF303)	[more]
AT3G53010.1	1.6e-07	36.90	Domain of unknown function (DUF303)	[more]

BLAST of Bhi04G000351 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9J9	2.9e-09	37.33	Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g...	[more]

BLAST of Bhi04G000351 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886575.1	5.3e-37	58.39	probable carbohydrate esterase At4g34215 [Benincasa hispida]	[more]
KAA0066252.1	4.5e-36	68.75	putative carbohydrate esterase [Cucumis melo var. makuwa] >TYJ97669.1 putative c...	[more]
XP_038886442.1	1.0e-35	56.29	probable carbohydrate esterase At4g34215 [Benincasa hispida]	[more]
XP_023002177.1	2.7e-33	54.25	probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 prob...	[more]
XP_023002892.1	2.7e-33	54.25	probable carbohydrate esterase At4g34215 [Cucurbita maxima]	[more]

BLAST of Bhi04G000351 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BF43	2.2e-36	68.75	Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1KIR8	1.3e-33	54.25	probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1KKV2	1.3e-33	54.25	probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A5A7VGP3	1.7e-33	52.98	Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A5A7VGI5	1.7e-33	52.98	Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005181	Sialate O-acetylesterase domain	PFAM	PF03629	SASA	coord: 32..108 e-value: 6.5E-19 score: 68.5
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 25..111 e-value: 6.8E-17 score: 64.1
None	No IPR available	PANTHER	PTHR31988:SF19	BNACNNG62850D PROTEIN	coord: 30..109
None	No IPR available	PANTHER	PTHR31988	ESTERASE, PUTATIVE (DUF303)-RELATED	coord: 30..109
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 37..107

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M000351	Bhi04M000351	mRNA