Bhi04G000333 (gene) Wax gourd (B227) v1

Overview
NameBhi04G000333
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionHeat stress transcription factor B-4
Locationchr4: 8899887 .. 8901922 (-)
RNA-Seq ExpressionBhi04G000333
SyntenyBhi04G000333
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATATTCTCTCTCTCACTAACCGAGGAGAGAGAAATTCATTTAATAAATAAATAAATAAAACACACTAATAGTAACAAGAGTAAAACTTGAATGCAACTTAGAGTACAGTTATCTTTATGCATTTTTTCAATGCATCAAAGTATATTTTTTACGATAATAATAAAGTTAAAAAGAAAATGTTGAGATTACAGTTACAGATTTCCACAATAACGTCACATCCTTTCTCTTCTGTGTGTCACCAAAATTCATTTCTTTTATCCCACACACACTCAGTCCCTCTCATTCCTTAACTTTTGTTTTTGTTTCTTAGAAGAAATTTCAAAAGTGAGTTTCCATTCCAATTCCCTTCTTCTTCTTCTGATCTTCTTCATCAAACCATGTCTGTAATGCTTGACAATTGTGAAGGCATCTTACTTTCTTTGGACTCTCACAAGTCTGTACCTGCCCCTTTCCTCTCCAAAACCTATCAATTAGTCGACGACCCCTCGACCGATCACATTGTCTCTTGGGGCGAAGACGACACCACTTTCGTCGTCTGGCGCCCCCCCGAGTTCGCCCGCGATCTCCTCCCCAACTACTTCAAACACAACAACTTCTCTAGCTTTGTTCGTCAACTCAACACTTATGTATGTAATTCACAACTCCATATTCTTTTCTTTTAGAAAGATACTAAAGTAACCATGGATTAGTATATGCACTAGAATATCTCAATTAAATTAACACATATTTTGCACTCTCATCATTAAAATCACTACGGAGAAGAGTTGGAGAAAGAGCTCGAGAGTTCATACAATGAACATGGCTTAATTTGGAGTGTTTTCTAACATGTTTTTTTTTTTTTTTTTTTCATTAGGGTTTTAGGAAGATTGTGCCAGACAGATGGGAGTTTGCGAATGAGTTCTTCAGAAAAGGTGAGAAGCATTTGTTGTGTGAGATTCATAGACGAAAAACAGCTCAGCCTCAACTGCCTTACAGCCATCACCATCTTGGAAATCTCAATGCTCCTAGTTTTTTCCCGTTTTCGACTCGAGTTAGCATCTCGCCGTCAGATTCAGACGAGCAGATCAATTGGTGCGACTCACCACCAGCACCACCGAAAGGTGGGGCGAGTGGATATTCGGTTATGGGAAGTGGAATTATTAGTAACAATAGTAGTACGAGTAGTTACAATATGATGAGTTCGGTGACAGCTTTGTCTGAGGATAATGAGAGGTTGAGGAGGAATAACTCTATGCTGATTTCGGAGCTTACTCATATGAGGAAACTTTATAATGATATTATTTATTTTGTTCAAAACCATGTCAAGCCTGTTTCTCCTAGCAATTCTTTCCCTTCTTTACTTCTTTGCCATCCTTCTTCTACTACTCCTAATTCCCAGAAACCCCTTAATCATTTTCTTGGATCCTCTCCTTCTACTACTCATTCTAATACTGCTACTATTACTACTAACCACTTTTCTATTCCTGCTACTATCGGTACGCCTCATTTTGTCAGCTCTCCAACTGCAACGTCGAAGAGTGCAGTTACAGAGCTCGACGTTGGGGAGGCTGATAACAATAGTTGCAAAACCAAGCTGTTTGGAGTGCCGATTTCATCGTCCAAGAAAAGGATGCATCCTGATACTACTAATTACGTTTCTATTTCTTCCAGTGTCTCAGATACCAACAAGAATTCTCGCTTGCTCTTGGAAAAAGGTGACAATTTGGGGCTCAATCTTATGCCTCCTTCCACTTCCTAGCCATTGCTCATCCTCCAATACCCTCTTTTTTCTTTTTCAATTCTCTTAAGATTATTCAATTTTTTATTATTCCTTTTTTTTCTTTTGTTATTTTTGAGCGAATCGGTTCGGTTCAGTTCACTTCAAGAACTATTGGAGTATTTTGTGTGAGGAAGTTGTGAAAATGTGTATGTGATTATCTTGTACATCTCCATCTTTGTTATTCTAATGCTCATGAAAGTTAGTTAATTAAACTCGCAGGGCTTTTGTTTTTTAGTAAAGCAACCGTAGGGATGGGATCGAACCTTTTAACCTTCA

mRNA sequence

CATATTCTCTCTCTCACTAACCGAGGAGAGAGAAATTCATTTAATAAATAAATAAATAAAACACACTAATAGTAACAAGAGTAAAACTTGAATGCAACTTAGAGTACAGTTATCTTTATGCATTTTTTCAATGCATCAAAGTATATTTTTTACGATAATAATAAAGTTAAAAAGAAAATGTTGAGATTACAGTTACAGATTTCCACAATAACGTCACATCCTTTCTCTTCTGTGTGTCACCAAAATTCATTTCTTTTATCCCACACACACTCAGTCCCTCTCATTCCTTAACTTTTGTTTTTGTTTCTTAGAAGAAATTTCAAAAGTGAGTTTCCATTCCAATTCCCTTCTTCTTCTTCTGATCTTCTTCATCAAACCATGTCTGTAATGCTTGACAATTGTGAAGGCATCTTACTTTCTTTGGACTCTCACAAGTCTGTACCTGCCCCTTTCCTCTCCAAAACCTATCAATTAGTCGACGACCCCTCGACCGATCACATTGTCTCTTGGGGCGAAGACGACACCACTTTCGTCGTCTGGCGCCCCCCCGAGTTCGCCCGCGATCTCCTCCCCAACTACTTCAAACACAACAACTTCTCTAGCTTTGTTCGTCAACTCAACACTTATGGTTTTAGGAAGATTGTGCCAGACAGATGGGAGTTTGCGAATGAGTTCTTCAGAAAAGGTGAGAAGCATTTGTTGTGTGAGATTCATAGACGAAAAACAGCTCAGCCTCAACTGCCTTACAGCCATCACCATCTTGGAAATCTCAATGCTCCTAGTTTTTTCCCGTTTTCGACTCGAGTTAGCATCTCGCCGTCAGATTCAGACGAGCAGATCAATTGGTGCGACTCACCACCAGCACCACCGAAAGGTGGGGCGAGTGGATATTCGGTTATGGGAAGTGGAATTATTAGTAACAATAGTAGTACGAGTAGTTACAATATGATGAGTTCGGTGACAGCTTTGTCTGAGGATAATGAGAGGTTGAGGAGGAATAACTCTATGCTGATTTCGGAGCTTACTCATATGAGGAAACTTTATAATGATATTATTTATTTTGTTCAAAACCATGTCAAGCCTGTTTCTCCTAGCAATTCTTTCCCTTCTTTACTTCTTTGCCATCCTTCTTCTACTACTCCTAATTCCCAGAAACCCCTTAATCATTTTCTTGGATCCTCTCCTTCTACTACTCATTCTAATACTGCTACTATTACTACTAACCACTTTTCTATTCCTGCTACTATCGGTACGCCTCATTTTGTCAGCTCTCCAACTGCAACGTCGAAGAGTGCAGTTACAGAGCTCGACGTTGGGGAGGCTGATAACAATAGTTGCAAAACCAAGCTGTTTGGAGTGCCGATTTCATCGTCCAAGAAAAGGATGCATCCTGATACTACTAATTACGTTTCTATTTCTTCCAGTGTCTCAGATACCAACAAGAATTCTCGCTTGCTCTTGGAAAAAGGTGACAATTTGGGGCTCAATCTTATGCCTCCTTCCACTTCCTAGCCATTGCTCATCCTCCAATACCCTCTTTTTTCTTTTTCAATTCTCTTAAGATTATTCAATTTTTTATTATTCCTTTTTTTTCTTTTGTTATTTTTGAGCGAATCGGTTCGGTTCAGTTCACTTCAAGAACTATTGGAGTATTTTGTGTGAGGAAGTTGTGAAAATGTGTATGTGATTATCTTGTACATCTCCATCTTTGTTATTCTAATGCTCATGAAAGTTAGTTAATTAAACTCGCAGGGCTTTTGTTTTTTAGTAAAGCAACCGTAGGGATGGGATCGAACCTTTTAACCTTCA

Coding sequence (CDS)

ATGTCTGTAATGCTTGACAATTGTGAAGGCATCTTACTTTCTTTGGACTCTCACAAGTCTGTACCTGCCCCTTTCCTCTCCAAAACCTATCAATTAGTCGACGACCCCTCGACCGATCACATTGTCTCTTGGGGCGAAGACGACACCACTTTCGTCGTCTGGCGCCCCCCCGAGTTCGCCCGCGATCTCCTCCCCAACTACTTCAAACACAACAACTTCTCTAGCTTTGTTCGTCAACTCAACACTTATGGTTTTAGGAAGATTGTGCCAGACAGATGGGAGTTTGCGAATGAGTTCTTCAGAAAAGGTGAGAAGCATTTGTTGTGTGAGATTCATAGACGAAAAACAGCTCAGCCTCAACTGCCTTACAGCCATCACCATCTTGGAAATCTCAATGCTCCTAGTTTTTTCCCGTTTTCGACTCGAGTTAGCATCTCGCCGTCAGATTCAGACGAGCAGATCAATTGGTGCGACTCACCACCAGCACCACCGAAAGGTGGGGCGAGTGGATATTCGGTTATGGGAAGTGGAATTATTAGTAACAATAGTAGTACGAGTAGTTACAATATGATGAGTTCGGTGACAGCTTTGTCTGAGGATAATGAGAGGTTGAGGAGGAATAACTCTATGCTGATTTCGGAGCTTACTCATATGAGGAAACTTTATAATGATATTATTTATTTTGTTCAAAACCATGTCAAGCCTGTTTCTCCTAGCAATTCTTTCCCTTCTTTACTTCTTTGCCATCCTTCTTCTACTACTCCTAATTCCCAGAAACCCCTTAATCATTTTCTTGGATCCTCTCCTTCTACTACTCATTCTAATACTGCTACTATTACTACTAACCACTTTTCTATTCCTGCTACTATCGGTACGCCTCATTTTGTCAGCTCTCCAACTGCAACGTCGAAGAGTGCAGTTACAGAGCTCGACGTTGGGGAGGCTGATAACAATAGTTGCAAAACCAAGCTGTTTGGAGTGCCGATTTCATCGTCCAAGAAAAGGATGCATCCTGATACTACTAATTACGTTTCTATTTCTTCCAGTGTCTCAGATACCAACAAGAATTCTCGCTTGCTCTTGGAAAAAGGTGACAATTTGGGGCTCAATCTTATGCCTCCTTCCACTTCCTAG

Protein sequence

MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIISNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSNSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSPTATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTNKNSRLLLEKGDNLGLNLMPPSTS
Homology
BLAST of Bhi04G000333 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 317.8 bits (813), Expect = 1.2e-86
Identity = 195/371 (52.56%), Postives = 237/371 (63.88%), Query Frame = 0

Query: 19  KSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 78
           K+VPAPFL+KTYQLVDDP+TDH+VSWG+DDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ--PQ--LPYSHHHLGNLNAP 138
           QLNTYGFRKIVPDRWEFANEFF++GEKHLLCEIHRRKT+Q  PQ   P+  HH      P
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 ----SFFPFSTRVSISPSDSDEQINWC-DSPPAPPKGGASGYSVMGSGIISNNSSTSSYN 198
               SFFP       +P   +E   WC DSPP+ P+            +I     T+   
Sbjct: 149 FSGGSFFPLPPPRVTTP---EEDHYWCDDSPPSRPR------------VIPQQIDTA--- 208

Query: 199 MMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSNSFPSLLLCH 258
             + VTALSEDNERLRR+N++L+SEL HM+KLYNDIIYFVQNHVKPV+PSN+   L    
Sbjct: 209 --AQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNNSSYL---- 268

Query: 259 PSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSPTATSKSAVTE 318
            SS     Q+         P+  + NTAT+   + +          ++S   TS+S++T 
Sbjct: 269 -SSFLQKQQQQ------QPPTLDYYNTATVNATNLNA---------LNSSPPTSQSSITV 328

Query: 319 LDVGEAD----NNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTNKNSRLLLEKGD 377
           L+    +    +N  KTKLFGV + SSKKR H             SD    +RL+L++ D
Sbjct: 329 LEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSH-----------HFSDQTSKTRLVLDQSD 347

BLAST of Bhi04G000333 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.2e-49
Identity = 108/222 (48.65%), Postives = 143/222 (64.41%), Query Frame = 0

Query: 16  DSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           DS +S+P PFL+KTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLPYSHHHLGNLNAPS 135
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +QP +  +        A S
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 136 -----FFPFSTRVSISPSDS-DEQINWCDSPPAPPKGGASGYSVMGSGIISNNSSTSSYN 195
                  P    + +SPS+S +EQ+   +S PA       G  V+G G +   +S     
Sbjct: 171 AVTVAAVPVVAHI-VSPSNSGEEQVISSNSSPAAAAAAIGG--VVGGGSLQRTTS----- 230

Query: 196 MMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQN 232
             ++   L E+NERLR++N  L  E+T ++ LY +I   + N
Sbjct: 231 -CTTAPELVEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

BLAST of Bhi04G000333 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 173.7 bits (439), Expect = 2.8e-43
Identity = 99/218 (45.41%), Postives = 132/218 (60.55%), Query Frame = 0

Query: 17  SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + +SVPAPFLSKTYQLVDD STD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLPYSHHHLGNLNAPSF 136
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK+           +G       
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVG------- 126

Query: 137 FPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIISNNSSTSSYNMMSSVTA 196
                    SPS+S+              GG   +    S   S  SS +  ++ + V  
Sbjct: 127 ---------SPSESN-------------SGGGDDHG--SSSTSSPGSSKNPGSVENMVAD 186

Query: 197 LSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVK 235
           LS +NE+L+R N+ L SEL   +K  ++++ F+  H+K
Sbjct: 187 LSGENEKLKRENNNLSSELAAAKKQRDELVTFLTGHLK 193

BLAST of Bhi04G000333 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 170.6 bits (431), Expect = 2.4e-42
Identity = 95/217 (43.78%), Postives = 134/217 (61.75%), Query Frame = 0

Query: 17  SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S +S+P PFL+KT+ LV+D S D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLPYSHHHLGNLNAPSF 136
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK     +  +H     + APS 
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK-----ITTTHQ---TVVAPSS 135

Query: 137 FPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIISNNSSTSSYNMMSSVTA 196
              +  + +SPS+S E  N      + P           S    + + T+    +S    
Sbjct: 136 EQRNQTMVVSPSNSGEDNNNNQVMSSSP-----------SSWYCHQTKTTGNGGLS--VE 195

Query: 197 LSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHV 234
           L E+NE+LR  N  L  ELT M+ + ++I   + N+V
Sbjct: 196 LLEENEKLRSQNIQLNRELTQMKSICDNIYSLMSNYV 211

BLAST of Bhi04G000333 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-39
Identity = 71/99 (71.72%), Postives = 84/99 (84.85%), Query Frame = 0

Query: 20  SVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 79
           S+P PFLSKTY +V+DP+TD IVSW   + +F+VW PPEF+RDLLP YFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 80  LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ 119
           LNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+ Q
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQ 146

BLAST of Bhi04G000333 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.7e-85
Identity = 195/371 (52.56%), Postives = 237/371 (63.88%), Query Frame = 0

Query: 19  KSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 78
           K+VPAPFL+KTYQLVDDP+TDH+VSWG+DDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ--PQ--LPYSHHHLGNLNAP 138
           QLNTYGFRKIVPDRWEFANEFF++GEKHLLCEIHRRKT+Q  PQ   P+  HH      P
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 ----SFFPFSTRVSISPSDSDEQINWC-DSPPAPPKGGASGYSVMGSGIISNNSSTSSYN 198
               SFFP       +P   +E   WC DSPP+ P+            +I     T+   
Sbjct: 149 FSGGSFFPLPPPRVTTP---EEDHYWCDDSPPSRPR------------VIPQQIDTA--- 208

Query: 199 MMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSNSFPSLLLCH 258
             + VTALSEDNERLRR+N++L+SEL HM+KLYNDIIYFVQNHVKPV+PSN+   L    
Sbjct: 209 --AQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNNSSYL---- 268

Query: 259 PSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSPTATSKSAVTE 318
            SS     Q+         P+  + NTAT+   + +          ++S   TS+S++T 
Sbjct: 269 -SSFLQKQQQQ------QPPTLDYYNTATVNATNLNA---------LNSSPPTSQSSITV 328

Query: 319 LDVGEAD----NNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTNKNSRLLLEKGD 377
           L+    +    +N  KTKLFGV + SSKKR H             SD    +RL+L++ D
Sbjct: 329 LEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSH-----------HFSDQTSKTRLVLDQSD 347

BLAST of Bhi04G000333 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.9e-69
Identity = 146/268 (54.48%), Postives = 177/268 (66.04%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLD----------SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTT 60
           M+ +++ C  +++S++          + K VPAPFL+KTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQ------PQLPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPP 180
           IHRRK++Q      P  PY HHH  +LN     PFS                   PP PP
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHH--HLN-----PFSL------------------PPPPP 180

Query: 181 KGGASGYSVMGSGIISNNSSTSSYNMMSS--------VTALSEDNERLRRNNSMLISELT 240
                  +     +I    +T+++  ++         + ALSEDN +LRR NS+L+SEL 
Sbjct: 181 -------AYHHHHLIQEEPATTAHCTVAGDGGEGGDFLAALSEDNRQLRRRNSLLLSELA 236

Query: 241 HMRKLYNDIIYFVQNHVKPVSPSNSFPS 245
           HM+KLYNDIIYF+QNHV PV+ + + PS
Sbjct: 241 HMKKLYNDIIYFLQNHVAPVTTTTTTPS 236

BLAST of Bhi04G000333 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.7e-66
Identity = 135/227 (59.47%), Postives = 154/227 (67.84%), Query Frame = 0

Query: 20  SVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 79
           + PAPFLSKTYQLVDDPSTD +VSWGED+ TFVVWRPPEFARDLLPNYFKHNNFSSFVRQ
Sbjct: 32  AAPAPFLSKTYQLVDDPSTDDVVSWGEDEATFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 91

Query: 80  LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ-------PQLPYSHHHLGNLN 139
           LNTYGFRKIV DRWEFANEFFRKG KHLL EIHRRK++        P  P   H+  +L 
Sbjct: 92  LNTYGFRKIVADRWEFANEFFRKGAKHLLSEIHRRKSSSCSQPQPPPPFPMHQHYPLSLF 151

Query: 140 APSFFPFSTRVSISPSDSDE-QINWCDSPPAPPKGGASGYSVMGSGIISNNSSTSSYNMM 199
           +P   P S  V  + + +   Q  +C SP     GG                        
Sbjct: 152 SPPTTPRSPPVGAAAAAAYHFQEEYCSSPADYAGGGG----------------------- 211

Query: 200 SSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSP 239
             + ALSEDN +LRR NS+L+SEL HMRKLYNDIIYF+QNHV+PV+P
Sbjct: 212 DLLAALSEDNRQLRRRNSLLLSELAHMRKLYNDIIYFLQNHVEPVAP 235

BLAST of Bhi04G000333 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.8e-63
Identity = 171/389 (43.96%), Postives = 215/389 (55.27%), Query Frame = 0

Query: 17  SHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDD-TTFVVWRPPEFARDLLPNYFKHNNFSS 76
           + K+VPAPFL+KTYQLVDDP+TDHIVSWG+D  +TFVVWRPPEFARD+LPNYFKHNNFSS
Sbjct: 17  AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILPNYFKHNNFSS 76

Query: 77  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRK-----TAQPQLP--YSHHHL 136
           FVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRK     TA P  P  ++  H 
Sbjct: 77  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTASPSPPPFFAPPHF 136

Query: 137 GNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVM-------GSGIISN 196
              + P         +    D     +    P   P        V        G     +
Sbjct: 137 PLFHHPGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRLLALGGPAPSPS 196

Query: 197 NSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSNS 256
           ++        ++   L E+NERLRR+N+ L+ EL HMRKLYNDIIYFVQNHV+PV+PS +
Sbjct: 197 SAEAGGAGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDIIYFVQNHVRPVAPSPA 256

Query: 257 FPSLL--LCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSP 316
             + L  L   +   P +   LN+   S  STT S++ TI                  SP
Sbjct: 257 AAAFLQGLGMQARKKPAAANVLNN---SGGSTTSSSSLTIAEE--------------PSP 316

Query: 317 TATSKSAVTELDVGEADN-----NSCKTKLFGVPISS------SKKRMHPDTTNYVSISS 376
               +    E   GEA N     +S  TKLFGV +S+      SK+   P+     S ++
Sbjct: 317 PPQQQHLAGEKSGGEAGNSSAARSSAPTKLFGVHLSAAPCGAGSKRASSPEEHPPTSPAT 376

Query: 377 SVSDTNKNSRLLLEKGDNLGLNLMPPSTS 378
                    RL+LE  D+L L + P S+S
Sbjct: 377 -------KPRLVLE-CDDLSLTVAPSSSS 380

BLAST of Bhi04G000333 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.3e-58
Identity = 157/373 (42.09%), Postives = 213/373 (57.10%), Query Frame = 0

Query: 21  VPAPFLSKTYQLVDDPSTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHNNFSS 80
           VPAPFL+KTYQLVDDP+TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+NFSS
Sbjct: 22  VPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHSNFSS 81

Query: 81  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ------PQLPYSHHHLG 140
           FVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A       P  P       
Sbjct: 82  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPFFAPR 141

Query: 141 NLNAPSFFPFSTRVSI----SPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIISNNSST 200
           +  A +FF     +      +   + E+ +W +S   P    +   S +G  I     + 
Sbjct: 142 HFAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPARRAA 201

Query: 201 SSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSNSFPSL 260
           ++        AL ++N RL R N+ L+ EL HMRKLY+DIIYFVQNHV+PV+PS +  + 
Sbjct: 202 AT----PEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPSPAAAAA 261

Query: 261 L--LCHPSSTTPNSQKPLNHFLGSS-PSTTHSNTATITTNHFSIPATIGTPHFVSSPTAT 320
           L  L          + P +   G+S  S T S++ T+  +  ++ A       +  P  T
Sbjct: 262 LHGLGVLRPPPAGGKAPASEVRGASGRSATSSSSLTVAEDQPTLLA-------LRLPRTT 321

Query: 321 SKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTN-KNSRLLL 375
            K  + E+  G   N    TKLFGV +SS+ ++     +   S       T+    R L+
Sbjct: 322 EK-IINEVSGG---NGGGSTKLFGVHLSSADEQTSSGASRKRSPPQEQPPTSPAPKRTLV 379

BLAST of Bhi04G000333 vs. NCBI nr
Match: XP_038885667.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 755.0 bits (1948), Expect = 3.1e-214
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIIS 180
           LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIIS
Sbjct: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIIS 180

Query: 181 NNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSN 240
           NNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSN
Sbjct: 181 NNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSN 240

Query: 241 SFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSPT 300
           SFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSPT
Sbjct: 241 SFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSPT 300

Query: 301 ATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTNKNSRLL 360
           ATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTNKNSRLL
Sbjct: 301 ATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTNKNSRLL 360

Query: 361 LEKGDNLGLNLMPPSTS 378
           LEKGDNLGLNLMPPSTS
Sbjct: 361 LEKGDNLGLNLMPPSTS 377

BLAST of Bhi04G000333 vs. NCBI nr
Match: XP_004143013.1 (heat stress transcription factor B-4 [Cucumis sativus] >KGN62495.1 hypothetical protein Csa_018602 [Cucumis sativus])

HSP 1 Score: 677.6 bits (1747), Expect = 6.4e-191
Identity = 346/379 (91.29%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKG-GASGYSVMGSGII 180
           LPY+HHHLGN+N PSFFPFSTRVSISPSDSDEQINWCDSPP PP+  G  GYSVMGSGII
Sbjct: 121 LPYTHHHLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPVPPRATGTGGYSVMGSGII 180

Query: 181 SNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPS 240
           SNNSSTS+YNMMSSVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVKPVSPS
Sbjct: 181 SNNSSTSNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPVSPS 240

Query: 241 NSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSP 300
           NSFPSLLL H  STT NSQKPLNHFLG SP     NT + TTNHFS+P TI T HFVSSP
Sbjct: 241 NSFPSLLLSHHPSTT-NSQKPLNHFLGISP----PNTTSTTTNHFSVPGTIVTTHFVSSP 300

Query: 301 TATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPD-TTNYVSISSSVSDTNKNSR 360
           TATSKSAVTELDVGEADNNSCKTKLFGV ISSSKKRMHPD TTNYVSI S+VSDTNKNSR
Sbjct: 301 TATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSR 360

Query: 361 LLLEKGDNLGLNLMPPSTS 378
           L+LEKGDNLGLNLMPPSTS
Sbjct: 361 LILEKGDNLGLNLMPPSTS 374

BLAST of Bhi04G000333 vs. NCBI nr
Match: KAA0060911.1 (heat stress transcription factor B-4 [Cucumis melo var. makuwa])

HSP 1 Score: 675.6 bits (1742), Expect = 2.4e-190
Identity = 344/379 (90.77%), Postives = 353/379 (93.14%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKG-GASGYSVMGSGII 180
           LPYSHHHLGN+N PSFFPFSTRVSISPSDSDEQINWCDSPP  P+  G  GYSVMGSGII
Sbjct: 121 LPYSHHHLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPLAPRATGTGGYSVMGSGII 180

Query: 181 SNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPS 240
           SNNSS S+YNMMSSVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVKPVSPS
Sbjct: 181 SNNSSASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPVSPS 240

Query: 241 NSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSP 300
           NSFPSLLL H  S+T NSQKPLNHFLG SP     NT + TTNHFS+P TI T HFVSSP
Sbjct: 241 NSFPSLLLSHHPSSTANSQKPLNHFLGISP----PNTTSTTTNHFSVPGTIVTTHFVSSP 300

Query: 301 TATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPD-TTNYVSISSSVSDTNKNSR 360
           TATSKSAVTELDVGEADNNSCKTKLFGV ISSSKKRMHPD TTNYVSI S+VSDTNKNSR
Sbjct: 301 TATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSR 360

Query: 361 LLLEKGDNLGLNLMPPSTS 378
           L+LEKGDNLGLNLMPPSTS
Sbjct: 361 LILEKGDNLGLNLMPPSTS 375

BLAST of Bhi04G000333 vs. NCBI nr
Match: XP_008444610.1 (PREDICTED: heat stress transcription factor B-4 [Cucumis melo])

HSP 1 Score: 674.1 bits (1738), Expect = 7.0e-190
Identity = 343/379 (90.50%), Postives = 352/379 (92.88%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKG-GASGYSVMGSGII 180
           LPYSHHHLGN+N PSFFPFSTRVSISPSDSDEQINWCDSPP  P+  G  GYSVMGSGII
Sbjct: 121 LPYSHHHLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPVAPRATGTGGYSVMGSGII 180

Query: 181 SNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPS 240
           SNNS  S+YNMMSSVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVKPVSPS
Sbjct: 181 SNNSRASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPVSPS 240

Query: 241 NSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSP 300
           NSFPSLLL H  S+T NSQKPLNHFLG SP     NT + TTNHFS+P TI T HFVSSP
Sbjct: 241 NSFPSLLLSHHPSSTANSQKPLNHFLGISP----PNTTSTTTNHFSVPGTIVTTHFVSSP 300

Query: 301 TATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPD-TTNYVSISSSVSDTNKNSR 360
           TATSKSAVTELDVGEADNNSCKTKLFGV ISSSKKRMHPD TTNYVSI S+VSDTNKNSR
Sbjct: 301 TATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSR 360

Query: 361 LLLEKGDNLGLNLMPPSTS 378
           L+LEKGDNLGLNLMPPSTS
Sbjct: 361 LILEKGDNLGLNLMPPSTS 375

BLAST of Bhi04G000333 vs. NCBI nr
Match: XP_022962526.1 (heat stress transcription factor B-4-like [Cucurbita moschata])

HSP 1 Score: 634.8 bits (1636), Expect = 4.7e-178
Identity = 327/385 (84.94%), Postives = 344/385 (89.35%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MS+MLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIIS 180
           LPY+HHHLG+L+  SFFPFSTRVSISPSDSDE INWCDSPP PP+   +G+SV+GS  +S
Sbjct: 121 LPYNHHHLGSLSGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLGSANVS 180

Query: 181 NNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSN 240
           NNS+T SY+MMSSVTALSEDNERLRRNNSMLISEL HMRKLYNDIIYFVQNHVKPVSPSN
Sbjct: 181 NNSNT-SYSMMSSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVSPSN 240

Query: 241 SFPSLLLCHPSSTT----PNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPA--TIGTPH 300
           SFPSLLL HPSSTT    PNSQKPLN  LG  P   H+NT   TT HFS P    I T H
Sbjct: 241 SFPSLLLSHPSSTTTPPPPNSQKPLNQLLGFYPPPPHANT---TTKHFSFPTANAIPTTH 300

Query: 301 FVSSPTATSKSAVTELDV--GEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSD 360
           FVSSPTATSKSAVTELD    E DN+SCKTKLFGVP+SSSKKR HPDTTNYVS SS+VSD
Sbjct: 301 FVSSPTATSKSAVTELDTFGMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVSTSSNVSD 360

Query: 361 TNKNSRLLLEKGDNLGLNLMPPSTS 378
           TNKNSRL+LEKGDNLGLNLMPPS+S
Sbjct: 361 TNKNSRLVLEKGDNLGLNLMPPSSS 381

BLAST of Bhi04G000333 vs. ExPASy TrEMBL
Match: A0A0A0LKU2 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356690 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.1e-191
Identity = 346/379 (91.29%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKG-GASGYSVMGSGII 180
           LPY+HHHLGN+N PSFFPFSTRVSISPSDSDEQINWCDSPP PP+  G  GYSVMGSGII
Sbjct: 121 LPYTHHHLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPVPPRATGTGGYSVMGSGII 180

Query: 181 SNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPS 240
           SNNSSTS+YNMMSSVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVKPVSPS
Sbjct: 181 SNNSSTSNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPVSPS 240

Query: 241 NSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSP 300
           NSFPSLLL H  STT NSQKPLNHFLG SP     NT + TTNHFS+P TI T HFVSSP
Sbjct: 241 NSFPSLLLSHHPSTT-NSQKPLNHFLGISP----PNTTSTTTNHFSVPGTIVTTHFVSSP 300

Query: 301 TATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPD-TTNYVSISSSVSDTNKNSR 360
           TATSKSAVTELDVGEADNNSCKTKLFGV ISSSKKRMHPD TTNYVSI S+VSDTNKNSR
Sbjct: 301 TATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSR 360

Query: 361 LLLEKGDNLGLNLMPPSTS 378
           L+LEKGDNLGLNLMPPSTS
Sbjct: 361 LILEKGDNLGLNLMPPSTS 374

BLAST of Bhi04G000333 vs. ExPASy TrEMBL
Match: A0A5A7V551 (Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00440 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 344/379 (90.77%), Postives = 353/379 (93.14%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKG-GASGYSVMGSGII 180
           LPYSHHHLGN+N PSFFPFSTRVSISPSDSDEQINWCDSPP  P+  G  GYSVMGSGII
Sbjct: 121 LPYSHHHLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPLAPRATGTGGYSVMGSGII 180

Query: 181 SNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPS 240
           SNNSS S+YNMMSSVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVKPVSPS
Sbjct: 181 SNNSSASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPVSPS 240

Query: 241 NSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSP 300
           NSFPSLLL H  S+T NSQKPLNHFLG SP     NT + TTNHFS+P TI T HFVSSP
Sbjct: 241 NSFPSLLLSHHPSSTANSQKPLNHFLGISP----PNTTSTTTNHFSVPGTIVTTHFVSSP 300

Query: 301 TATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPD-TTNYVSISSSVSDTNKNSR 360
           TATSKSAVTELDVGEADNNSCKTKLFGV ISSSKKRMHPD TTNYVSI S+VSDTNKNSR
Sbjct: 301 TATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSR 360

Query: 361 LLLEKGDNLGLNLMPPSTS 378
           L+LEKGDNLGLNLMPPSTS
Sbjct: 361 LILEKGDNLGLNLMPPSTS 375

BLAST of Bhi04G000333 vs. ExPASy TrEMBL
Match: A0A1S3BAP2 (heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103487881 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.4e-190
Identity = 343/379 (90.50%), Postives = 352/379 (92.88%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKG-GASGYSVMGSGII 180
           LPYSHHHLGN+N PSFFPFSTRVSISPSDSDEQINWCDSPP  P+  G  GYSVMGSGII
Sbjct: 121 LPYSHHHLGNINGPSFFPFSTRVSISPSDSDEQINWCDSPPVAPRATGTGGYSVMGSGII 180

Query: 181 SNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPS 240
           SNNS  S+YNMMSSVTALSEDNERLRRNNSMLISEL+HMRKLYNDIIYFVQNHVKPVSPS
Sbjct: 181 SNNSRASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPVSPS 240

Query: 241 NSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSSP 300
           NSFPSLLL H  S+T NSQKPLNHFLG SP     NT + TTNHFS+P TI T HFVSSP
Sbjct: 241 NSFPSLLLSHHPSSTANSQKPLNHFLGISP----PNTTSTTTNHFSVPGTIVTTHFVSSP 300

Query: 301 TATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPD-TTNYVSISSSVSDTNKNSR 360
           TATSKSAVTELDVGEADNNSCKTKLFGV ISSSKKRMHPD TTNYVSI S+VSDTNKNSR
Sbjct: 301 TATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSR 360

Query: 361 LLLEKGDNLGLNLMPPSTS 378
           L+LEKGDNLGLNLMPPSTS
Sbjct: 361 LILEKGDNLGLNLMPPSTS 375

BLAST of Bhi04G000333 vs. ExPASy TrEMBL
Match: A0A6J1HDI0 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111462929 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.3e-178
Identity = 327/385 (84.94%), Postives = 344/385 (89.35%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MS+MLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIIS 180
           LPY+HHHLG+L+  SFFPFSTRVSISPSDSDE INWCDSPP PP+   +G+SV+GS  +S
Sbjct: 121 LPYNHHHLGSLSGSSFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLGSANVS 180

Query: 181 NNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSN 240
           NNS+T SY+MMSSVTALSEDNERLRRNNSMLISEL HMRKLYNDIIYFVQNHVKPVSPSN
Sbjct: 181 NNSNT-SYSMMSSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVSPSN 240

Query: 241 SFPSLLLCHPSSTT----PNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPA--TIGTPH 300
           SFPSLLL HPSSTT    PNSQKPLN  LG  P   H+NT   TT HFS P    I T H
Sbjct: 241 SFPSLLLSHPSSTTTPPPPNSQKPLNQLLGFYPPPPHANT---TTKHFSFPTANAIPTTH 300

Query: 301 FVSSPTATSKSAVTELDV--GEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSD 360
           FVSSPTATSKSAVTELD    E DN+SCKTKLFGVP+SSSKKR HPDTTNYVS SS+VSD
Sbjct: 301 FVSSPTATSKSAVTELDTFGMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVSTSSNVSD 360

Query: 361 TNKNSRLLLEKGDNLGLNLMPPSTS 378
           TNKNSRL+LEKGDNLGLNLMPPS+S
Sbjct: 361 TNKNSRLVLEKGDNLGLNLMPPSSS 381

BLAST of Bhi04G000333 vs. ExPASy TrEMBL
Match: A0A6J1KEA4 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111492453 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.8e-174
Identity = 325/390 (83.33%), Postives = 341/390 (87.44%), Query Frame = 0

Query: 1   MSVMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MS+MLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MSLMLDNCEGILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQINWCDSPPAPPKGGASGYSVMGSGIIS 180
           LPY+HHHLG+    SFFPFSTRVSISPSDSDE INWCDSPP PP+   +G+SV+GS  +S
Sbjct: 121 LPYNHHHLGS----SFFPFSTRVSISPSDSDEHINWCDSPPLPPRAAPAGFSVLGSANVS 180

Query: 181 NNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSPSN 240
           NNS+T SY+MM SVTALSEDNERLRRNNSMLISEL HMRKLYNDIIYFVQNHVKPVSPSN
Sbjct: 181 NNSNT-SYSMMGSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVSPSN 240

Query: 241 SFPSLLLCHPSSTT---------PNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPA--T 300
           SFPSLLL HPS+TT         PNSQKPLN  LG  P  TH+NT   TT HFS P    
Sbjct: 241 SFPSLLLSHPSTTTTTPPPPPPPPNSQKPLNQLLGFYP-PTHANT---TTKHFSFPTANA 300

Query: 301 IGTPHFVSSPTATSKSAVTELDV--GEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSIS 360
           I T HFVSSPTATSKSAVTELD    E DN+SCKTKLFGVP+SSSKKR HPDTTNYVS S
Sbjct: 301 IPTTHFVSSPTATSKSAVTELDTFGMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVSTS 360

Query: 361 SSVSDTNKNSRLLLEKGDNLGLNLMPPSTS 378
           S VSDTNKNSRL+LEKGDNLGLNLMPPS+S
Sbjct: 361 SHVSDTNKNSRLVLEKGDNLGLNLMPPSSS 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G46264.11.2e-8652.56heat shock transcription factor B4 [more]
AT4G11660.11.2e-4948.65winged-helix DNA-binding transcription factor family protein [more]
AT4G36990.12.8e-4345.41heat shock factor 4 [more]
AT5G62020.12.4e-4243.78heat shock transcription factor B2A [more]
AT4G17750.11.1e-3971.72heat shock factor 1 [more]
Match NameE-valueIdentityDescription
Q9C6351.7e-8552.56Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ04.9e-6954.48Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX81.7e-6659.47Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U941.8e-6343.96Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R82.3e-5842.09Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
XP_038885667.13.1e-214100.00heat stress transcription factor B-4-like [Benincasa hispida][more]
XP_004143013.16.4e-19191.29heat stress transcription factor B-4 [Cucumis sativus] >KGN62495.1 hypothetical ... [more]
KAA0060911.12.4e-19090.77heat stress transcription factor B-4 [Cucumis melo var. makuwa][more]
XP_008444610.17.0e-19090.50PREDICTED: heat stress transcription factor B-4 [Cucumis melo][more]
XP_022962526.14.7e-17884.94heat stress transcription factor B-4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LKU23.1e-19191.29HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356690 ... [more]
A0A5A7V5511.2e-19090.77Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BAP23.4e-19090.50heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103487881 PE=... [more]
A0A6J1HDI02.3e-17884.94heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1KEA45.8e-17483.33heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 194..214
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 12..243
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 301..376
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 63..75
score: 78.68
coord: 76..88
score: 84.62
coord: 25..48
score: 47.26
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 1.2E-60
score: 217.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 3.0E-32
score: 111.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..116
e-value: 1.4E-37
score: 130.3
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 301..376
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..243
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M000333Bhi04M000333mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding