Bhi04G000328 (gene) Wax gourd (B227) v1

BLAST of Bhi04G000328 vs. TAIR 10
Match: AT5G11210.1 (glutamate receptor 2.5 )

HSP 1 Score: 227.3 bits (578), Expect = 3.1e-59
Identity = 171/589 (29.03%), Postives = 294/589 (49.92%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           M GV+G +TYF  +K  L   E +++K +  E        + + FA  AYDAA A+  ++
Sbjct: 205 MHGVLGVKTYFAKSK-ELLHLEARWQKRFGGE--------ELNNFACWAYDAATALAMSV 264

Query: 61  ENMGVENLS-----------------------SSSEELKEKILESNFEGVSGMVRFSKNN 120
           E +   N+S                        S  +L + +   +F+GV+G  RF   N
Sbjct: 265 EEIRHVNMSFNTTKEDTSRDDIGTDLDELGVALSGPKLLDALSTVSFKGVAG--RFQLKN 324

Query: 121 NGMLIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELS 180
             +   E+  FKII + +   + V FW  K G V KS+ ++K     S++  +  +    
Sbjct: 325 GKL---EATTFKIINIEESGERTVGFWKSKVGLV-KSLRVDK--VSHSSRRLRPIIWPGD 384

Query: 181 EMRVSKA-NSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIK 240
            + V K        +KLR AVP +     FV V+   N      +GF IDV   VM+ + 
Sbjct: 385 TIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMP 444

Query: 241 NISAYDLFPFN-------HSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTD 300
              +Y+  PF+        SYD+M+  V+  E+DGAVGD TILA R  +VDF + Y  T 
Sbjct: 445 YAVSYEYIPFDTPDGKPRGSYDEMVYNVFLGEFDGAVGDTTILANRSHYVDFALPYSETG 504

Query: 301 IVMVVTEKQEKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELK------G 360
           IV +V  K  K K  W F++     +WL+     +++  ++W+ E+Q ++E +       
Sbjct: 505 IVFLVPVKDGKEKGEWVFLKPLTKELWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDK 564

Query: 361 FGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSV 420
             ++ +FS S +F+ HR P ++   R+++  W F +L++T S++A+LTS++T    +P+V
Sbjct: 565 ISSVFYFSFSTLFFAHRRPSESFFTRVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTV 624

Query: 421 LDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKAL----KNGSIK 480
             ++ L++     +G  + SF +  L   + F+ S++K  NS ++  +       NG I 
Sbjct: 625 RHMDDLRK-SGVNIGYQTGSFTFERLKQ-MRFDESRLKTYNSPEEMRELFLHKSSNGGID 684

Query: 481 AAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNIS 540
           AAF    +  +F+AK C  Y+    +FK  G GFAF  GS L +++SR I  +T  + + 
Sbjct: 685 AAFDEVAYIKLFMAKYCSEYSIIEPTFKADGFGFAFPLGSPLVSDISRQILNITEGDAMK 744

Query: 541 TMEHK--LLESFKCSSSDKENGLGLGPEPFLNLFIICGSIALLVLMYIM 547
            +E+K  L E     S+  ++ + L    F  LF+I   +++++L+ ++
Sbjct: 745 AIENKWFLGEKHCLDSTTSDSPIQLDHHSFEALFLIVFVVSVILLLLML 774

BLAST of Bhi04G000328 vs. TAIR 10
Match: AT2G29100.1 (glutamate receptor 2.9 )

HSP 1 Score: 216.1 bits (549), Expect = 7.2e-56
Identity = 170/581 (29.26%), Postives = 275/581 (47.33%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           +EGV+G +++   +K  L  F  ++K+ ++ E P   +    ++FA+ AYD+  A+  A+
Sbjct: 270 IEGVLGVRSHVPKSK-ELGDFRLRWKRTFEKENPSMRD--DLNVFALWAYDSITALAKAV 329

Query: 61  E--------------------NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGM 120
           E                    ++G   +S     L++   E  F G++G  +     +G 
Sbjct: 330 EKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSEVRFNGLAGEFKLI---DGQ 389

Query: 121 LIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMR 180
           L  +SP F+II  V +  + + FWT + G +         +A  SNK     V    + +
Sbjct: 390 L--QSPKFEIINFVGNEERIIGFWTPRDGLM---------DATSSNKKTLGPVIWPGKSK 449

Query: 181 VSKANSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKN--I 240
           +         +KLR  VP +    +FV V+       K  +G++I++  A +  +    I
Sbjct: 450 IVPKGWEIPGKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEAALKELPYLVI 509

Query: 241 SAYDLFPFNHSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQ 300
             Y  F   ++Y++++  VY K +D  VGDITI A R  + DFT+ +  + + M+V  + 
Sbjct: 510 PEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTESGVSMMVPVRD 569

Query: 301 EKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKG-----FGNMVWFSVS 360
            + K  W F+E + L +W+      +F+ FV+WL EH+ N + +G      G  +WFS S
Sbjct: 570 NENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQYQIGTSLWFSFS 629

Query: 361 FIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMP 420
            + + HRE V + LAR V+  W F +LV+T S++ASLTS +T    QP+V +V  L +  
Sbjct: 630 TMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPTVTNVNDLIKNR 689

Query: 421 DATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKALKNG---SIKAAFFISPHADI 480
           D  VG    +F+ + L   L F   ++K  +S  D    L  G    I AAF    +   
Sbjct: 690 DC-VGYQGGAFVKDILLG-LGFHEDQLKPFDSAKDADDLLSKGKSKGIAAAFDEVAYLKA 749

Query: 481 FLAKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFK 540
            L+++C  Y     +FK GG GFAF K S L  E SR+I  LT  N    +E +     K
Sbjct: 750 ILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQIEDRWFPK-K 809

Query: 541 CSSSDKENGLG---LGPEPFLNLFIICGSIALLVLMYIMGL 549
               D    L    L    FL LF+I G+     L+  + L
Sbjct: 810 NDCPDPMTALSSNRLNLSSFLGLFLIAGTAISFSLLVFVAL 830

BLAST of Bhi04G000328 vs. TAIR 10
Match: AT2G29110.1 (glutamate receptor 2.8 )

HSP 1 Score: 208.8 bits (530), Expect = 1.2e-53
Identity = 165/580 (28.45%), Postives = 275/580 (47.41%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           ++GV+G +++   +K  L+ F  ++K+ +K E P   +    SIF + AYD+  A+  A+
Sbjct: 273 IDGVLGVRSHVPKSK-GLEDFRLRWKRNFKKENPWLRD--DLSIFGLWAYDSTTALAMAV 332

Query: 61  E--------------------NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGM 120
           E                    ++G  ++S     L E + E  F G++G  RF+  +  +
Sbjct: 333 EKTNISSFPYNNASGSSNNMTDLGTLHVSRYGPSLLEALSEIRFNGLAG--RFNLIDRQL 392

Query: 121 LIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMR 180
              ESP F+II  V +  + V FWT   G V  +V  NK  +    + G +     S + 
Sbjct: 393 ---ESPKFEIINFVGNEERIVGFWTPSNGLV--NVNSNKTTSFTGERFGPLIWPGKSTIV 452

Query: 181 VSKANSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISA 240
                     +K++  VP +     FV V            G++ID+  A +  +     
Sbjct: 453 PKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDPITNITTPKGYAIDIFEAALKKLPYSVI 512

Query: 241 YDLFPF---NHSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEK 300
              + F   +  YDD++  V +   D  VGD+TI A R  + DFT+ Y  + + M+V  +
Sbjct: 513 PQYYRFESPDDDYDDLVYKVDNGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMMVPVR 572

Query: 301 QEKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKG-----FGNMVWFSV 360
             + K  W F++ + L +W+      + + FV+WL EH+ N + +G      G   WFS 
Sbjct: 573 DNENKNTWVFLKPWGLDLWVTTACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSF 632

Query: 361 SFIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQM 420
           S + + HRE V + LAR V+  W F +LV+T S++A+LTS +T    QP+ ++V+ L + 
Sbjct: 633 STMVFAHREKVVSNLARFVVVVWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKN 692

Query: 421 PDATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFL 480
            D  VG    +F+ ++L     F  SK+K   S ++    L NGSI AAF    +    L
Sbjct: 693 GD-YVGYQHGAFVKDFLIKE-GFNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLRAIL 752

Query: 481 AKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHK-LLESFKC 540
           ++ C  Y     +FK  G GFAF + S L  +VS++I  +T  + +  +E+K  ++   C
Sbjct: 753 SQYCSKYAIVEPTFKTAGFGFAFPRNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQNDC 812

Query: 541 SSSDKENGLG---LGPEPFLNLFIICGSIALLVLMYIMGL 549
              D +  L    L    F  LF+I G  + L L+  + L
Sbjct: 813 --PDPKTALSSNRLSLRSFWGLFLIAGIASFLALLIFVFL 838

BLAST of Bhi04G000328 vs. TAIR 10
Match: AT2G29120.1 (glutamate receptor 2.7 )

HSP 1 Score: 201.4 bits (511), Expect = 1.8e-51
Identity = 157/575 (27.30%), Postives = 272/575 (47.30%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           M+GV+G +++   +K  LK+F  +++K+    +P++    + +IFA++AYD+  A+  A+
Sbjct: 282 MQGVLGVRSHIPKSK-KLKNFRLRWEKM----FPKKGNDEEMNIFALRAYDSITALAMAV 341

Query: 61  E--------------------NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGM 120
           E                    N+G   +S     L + +    F G++G        NG 
Sbjct: 342 EKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALSNVRFNGLAGEFELI---NGQ 401

Query: 121 LIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMR 180
           L  ES  F +I ++    + +  W    G V  + +     ++   ++G +     S+  
Sbjct: 402 L--ESSVFDVINIIGSEERIIGLWRPSNGIV--NAKSKNTTSVLGERLGPVIWPGKSKDV 461

Query: 181 VSKANSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNI--KNI 240
                     + LR  +P +    EFV+        A   +G+ I++  AV+  +    I
Sbjct: 462 PKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKLPYSVI 521

Query: 241 SAYDLF-PFNHSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEK 300
             Y  F   + +YD+M+  VY   YD  VGD+TI+A R  +VDFT+ Y  + + M+V  K
Sbjct: 522 PKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSMMVPLK 581

Query: 301 QEKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKG-----FGNMVWFSV 360
               K  W F+  + L +W+      +F+ F++W++EH+ N + +G      G   WF+ 
Sbjct: 582 DN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTSFWFAF 641

Query: 361 SFIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQM 420
           S + + HRE V + LAR V+  W F +LV+  S++A+LTS  T    QP+V + + L + 
Sbjct: 642 STMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWKDLIKF 701

Query: 421 PDATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFL 480
            +  +G    +F+   L S   F+ S++K   S  +  +   NG+I A+F    +  + L
Sbjct: 702 -NKNIGYQRGTFVRELLKSQ-GFDESQLKPFGSAVECDELFSNGTITASFDEVAYIKVIL 761

Query: 481 AKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFK-- 540
           ++N   YT    SFK  G GF F K S L  +VSR+I  +T    +  +E+K  +     
Sbjct: 762 SQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVTQGEEMQHIENKWFKKPNNC 821

Query: 541 --CSSSDKENGLGLGPEPFLNLFIICGSIALLVLM 544
              ++S   N L L    F  LF+I G  + L L+
Sbjct: 822 PDLNTSLSSNHLSL--SSFWGLFLIAGIASFLALL 838

BLAST of Bhi04G000328 vs. TAIR 10
Match: AT4G35290.2 (glutamate receptor 2 )

HSP 1 Score: 200.3 bits (508), Expect = 4.1e-51
Identity = 148/505 (29.31%), Postives = 249/505 (49.31%), Query Frame = 0

Query: 62  NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKEV 121
           N+G  ++     +  + I+ +N  GV+G ++F  + + +     P++ II VVD  ++++
Sbjct: 357 NLGALSIFDQGSQFLDYIVNTNMTGVTGQIQFLPDRSMI----QPSYDIINVVDDGFRQI 416

Query: 122 AFWTGKFGF-VEKSVEINKKEALRSNKMGKMG----VRELSEMRVSKANSNNGERKLRFA 181
            +W+   G  +     + KK + RS+    +         SE        NNG R+LR  
Sbjct: 417 GYWSNHSGLSIIPPESLYKKLSNRSSSNQHLNNVTWPGGTSETPRGWVFPNNG-RRLRIG 476

Query: 182 VPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPF-----NHSYD 241
           VP + + +EFV+    SN       G++IDV  A +  I     ++   F     N +++
Sbjct: 477 VPDRASFKEFVSRLDGSN----KVQGYAIDVFEAAVKLISYPVPHEFVLFGDGLKNPNFN 536

Query: 242 DMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMV--VTEKQEKWKRRWAFME 301
           + +  V    +D  VGDI I+ KR   VDFT  Y+ + +V+V  VT+  +     WAF+ 
Sbjct: 537 EFVNNVTIGVFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVVAPVTKLND---TPWAFLR 596

Query: 302 AFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKG-----FGNMVWFSVSFIFYMHREPVK 361
            F  P+W +     + V  VIW++EH+ NDE +G        ++WFS S +F+ HRE   
Sbjct: 597 PFTPPMWAVTAAFFLIVGSVIWILEHRINDEFRGPPRKQIVTILWFSFSTMFFSHRENTV 656

Query: 362 NGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESF 421
           + L R V+  WLF +L++T+S++ASLTS++T       +  V+TL       VG    S+
Sbjct: 657 STLGRAVLLIWLFVVLIITSSYTASLTSILTVQQLNSPIRGVDTLIS-SSGRVGFQVGSY 716

Query: 422 IYNYLNSTLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVS 481
             NY+   L    S++  + S  +Y  AL+NG++ A     P+ D+FL++ C G+     
Sbjct: 717 AENYMIDELNIARSRLVPLGSPKEYAAALQNGTVAAIVDERPYVDLFLSEFC-GFAIRGQ 776

Query: 482 SFKLGGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFKCS----SSDKENGL 541
            F   G GFAF + S L  ++S +I  L+    +  +  K L    CS    S   E+  
Sbjct: 777 EFTRSGWGFAFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLSRSNCSNLNGSVSDEDSE 836

Query: 542 GLGPEPFLNLFIICGSIALLVLMYI 546
            L    F  LF++CG I+  + ++I
Sbjct: 837 QLKLRSFWGLFLVCG-ISCFIALFI 846

BLAST of Bhi04G000328 vs. ExPASy Swiss-Prot
Match: Q9LFN5 (Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2)

HSP 1 Score: 227.3 bits (578), Expect = 4.4e-58
Identity = 171/589 (29.03%), Postives = 294/589 (49.92%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           M GV+G +TYF  +K  L   E +++K +  E        + + FA  AYDAA A+  ++
Sbjct: 279 MHGVLGVKTYFAKSK-ELLHLEARWQKRFGGE--------ELNNFACWAYDAATALAMSV 338

Query: 61  ENMGVENLS-----------------------SSSEELKEKILESNFEGVSGMVRFSKNN 120
           E +   N+S                        S  +L + +   +F+GV+G  RF   N
Sbjct: 339 EEIRHVNMSFNTTKEDTSRDDIGTDLDELGVALSGPKLLDALSTVSFKGVAG--RFQLKN 398

Query: 121 NGMLIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELS 180
             +   E+  FKII + +   + V FW  K G V KS+ ++K     S++  +  +    
Sbjct: 399 GKL---EATTFKIINIEESGERTVGFWKSKVGLV-KSLRVDK--VSHSSRRLRPIIWPGD 458

Query: 181 EMRVSKA-NSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIK 240
            + V K        +KLR AVP +     FV V+   N      +GF IDV   VM+ + 
Sbjct: 459 TIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMP 518

Query: 241 NISAYDLFPFN-------HSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTD 300
              +Y+  PF+        SYD+M+  V+  E+DGAVGD TILA R  +VDF + Y  T 
Sbjct: 519 YAVSYEYIPFDTPDGKPRGSYDEMVYNVFLGEFDGAVGDTTILANRSHYVDFALPYSETG 578

Query: 301 IVMVVTEKQEKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELK------G 360
           IV +V  K  K K  W F++     +WL+     +++  ++W+ E+Q ++E +       
Sbjct: 579 IVFLVPVKDGKEKGEWVFLKPLTKELWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDK 638

Query: 361 FGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSV 420
             ++ +FS S +F+ HR P ++   R+++  W F +L++T S++A+LTS++T    +P+V
Sbjct: 639 ISSVFYFSFSTLFFAHRRPSESFFTRVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTV 698

Query: 421 LDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKAL----KNGSIK 480
             ++ L++     +G  + SF +  L   + F+ S++K  NS ++  +       NG I 
Sbjct: 699 RHMDDLRK-SGVNIGYQTGSFTFERLKQ-MRFDESRLKTYNSPEEMRELFLHKSSNGGID 758

Query: 481 AAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNIS 540
           AAF    +  +F+AK C  Y+    +FK  G GFAF  GS L +++SR I  +T  + + 
Sbjct: 759 AAFDEVAYIKLFMAKYCSEYSIIEPTFKADGFGFAFPLGSPLVSDISRQILNITEGDAMK 818

Query: 541 TMEHK--LLESFKCSSSDKENGLGLGPEPFLNLFIICGSIALLVLMYIM 547
            +E+K  L E     S+  ++ + L    F  LF+I   +++++L+ ++
Sbjct: 819 AIENKWFLGEKHCLDSTTSDSPIQLDHHSFEALFLIVFVVSVILLLLML 848

BLAST of Bhi04G000328 vs. ExPASy Swiss-Prot
Match: Q9LFN8 (Glutamate receptor 2.6 OS=Arabidopsis thaliana OX=3702 GN=GLR2.6 PE=2 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 1.6e-55
Identity = 169/593 (28.50%), Postives = 290/593 (48.90%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           M GV+G +TYF+ +K  L   ET+++K +  E        + + F    YD A A+  ++
Sbjct: 278 MHGVLGVKTYFSRSK-ELMYLETRWRKRFGGE--------ELNNFECWGYDTATALAMSI 337

Query: 61  ENMGVENLSSSSEELKE---------------------KILES----NFEGVSGMVRFSK 120
           E +   N++ S  + K                      K+L++    +F+GV+G  RF  
Sbjct: 338 EEIS-SNVNMSFSQTKRNTSRDDTGTDLDDLSFALSGPKLLQALATVSFKGVAG--RFQL 397

Query: 121 NNNGMLIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMG--V 180
            N  +   E+  FKI+ + +   + V FW  K G V KS+ +N+     S+   ++   +
Sbjct: 398 KNGKL---EATTFKIVNIEESGERTVGFWKSKVGLV-KSLRVNQTGIKISHSSHRLRPII 457

Query: 181 RELSEMRVSKA-NSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVM 240
                + V K        +KLR AVP +     FV V+  +N  A   +GF IDV    M
Sbjct: 458 WPGDTIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDANTNAPTITGFCIDVFDTAM 517

Query: 241 NNIKNISAYDLFPF-------NHSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAY 300
             +     Y+  PF         SYD+M+  V+  E+DGAVGD TILA R  +VDF + Y
Sbjct: 518 RQMPYAVPYEYIPFETPDGKPRGSYDEMVYHVFLGEFDGAVGDTTILANRSTYVDFALPY 577

Query: 301 LNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDE----- 360
             T IV+VV  K E+ K +W F++     +W L     +++  ++W+ E+Q + +     
Sbjct: 578 SETGIVVVVPVKDEREKGKWVFLKPLTRELWFLTAASFLYIGIMVWIFEYQASGDFRKQS 637

Query: 361 -LKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWS 420
            +    N+ +FS S +F+ H  P ++   R+++  W F +L++T S++A+LTS++T    
Sbjct: 638 IINKISNVFYFSFSTLFFAHMRPSESIFTRVLVVVWCFVLLILTQSYTATLTSMLTVQEL 697

Query: 421 QPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDD----YPKALKN 480
           +P+V  ++ L+      +G  + SF +  L   + ++ S++K  ++  +    + K   N
Sbjct: 698 RPTVRHMDDLRN-SGVNIGYQTGSFTFERLKQ-MGYKESRLKTYDTPQEMHELFLKKSSN 757

Query: 481 GSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLA 540
           G I AAF    +  +F+AK C  YT    +FK  G GFAF  GS L  ++SR I  +T  
Sbjct: 758 GGIDAAFDEVAYVKLFMAKYCSKYTIIEPTFKADGFGFAFPLGSPLVPDLSRQILNITEG 817

Query: 541 NNISTMEHKLL--ESFKCSSSDKENGLGLGPEPFLNLFIICGSIALLVLMYIM 547
             +  +E+K L  E     S+  ++ + L    F  LF I   +++L+L+ ++
Sbjct: 818 ETMKAIENKWLLGEKHCLDSTTSDSPIRLDHHSFEALFTIVFVVSMLLLLAML 852

BLAST of Bhi04G000328 vs. ExPASy Swiss-Prot
Match: O81078 (Glutamate receptor 2.9 OS=Arabidopsis thaliana OX=3702 GN=GLR2.9 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.0e-54
Identity = 170/581 (29.26%), Postives = 275/581 (47.33%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           +EGV+G +++   +K  L  F  ++K+ ++ E P   +    ++FA+ AYD+  A+  A+
Sbjct: 270 IEGVLGVRSHVPKSK-ELGDFRLRWKRTFEKENPSMRD--DLNVFALWAYDSITALAKAV 329

Query: 61  E--------------------NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGM 120
           E                    ++G   +S     L++   E  F G++G  +     +G 
Sbjct: 330 EKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSEVRFNGLAGEFKLI---DGQ 389

Query: 121 LIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMR 180
           L  +SP F+II  V +  + + FWT + G +         +A  SNK     V    + +
Sbjct: 390 L--QSPKFEIINFVGNEERIIGFWTPRDGLM---------DATSSNKKTLGPVIWPGKSK 449

Query: 181 VSKANSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKN--I 240
           +         +KLR  VP +    +FV V+       K  +G++I++  A +  +    I
Sbjct: 450 IVPKGWEIPGKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEAALKELPYLVI 509

Query: 241 SAYDLFPFNHSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQ 300
             Y  F   ++Y++++  VY K +D  VGDITI A R  + DFT+ +  + + M+V  + 
Sbjct: 510 PEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTESGVSMMVPVRD 569

Query: 301 EKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKG-----FGNMVWFSVS 360
            + K  W F+E + L +W+      +F+ FV+WL EH+ N + +G      G  +WFS S
Sbjct: 570 NENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQYQIGTSLWFSFS 629

Query: 361 FIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMP 420
            + + HRE V + LAR V+  W F +LV+T S++ASLTS +T    QP+V +V  L +  
Sbjct: 630 TMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPTVTNVNDLIKNR 689

Query: 421 DATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKALKNG---SIKAAFFISPHADI 480
           D  VG    +F+ + L   L F   ++K  +S  D    L  G    I AAF    +   
Sbjct: 690 DC-VGYQGGAFVKDILLG-LGFHEDQLKPFDSAKDADDLLSKGKSKGIAAAFDEVAYLKA 749

Query: 481 FLAKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFK 540
            L+++C  Y     +FK GG GFAF K S L  E SR+I  LT  N    +E +     K
Sbjct: 750 ILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQIEDRWFPK-K 809

Query: 541 CSSSDKENGLG---LGPEPFLNLFIICGSIALLVLMYIMGL 549
               D    L    L    FL LF+I G+     L+  + L
Sbjct: 810 NDCPDPMTALSSNRLNLSSFLGLFLIAGTAISFSLLVFVAL 830

BLAST of Bhi04G000328 vs. ExPASy Swiss-Prot
Match: Q9C5V5 (Glutamate receptor 2.8 OS=Arabidopsis thaliana OX=3702 GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 1.6e-52
Identity = 165/580 (28.45%), Postives = 275/580 (47.41%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           ++GV+G +++   +K  L+ F  ++K+ +K E P   +    SIF + AYD+  A+  A+
Sbjct: 273 IDGVLGVRSHVPKSK-GLEDFRLRWKRNFKKENPWLRD--DLSIFGLWAYDSTTALAMAV 332

Query: 61  E--------------------NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGM 120
           E                    ++G  ++S     L E + E  F G++G  RF+  +  +
Sbjct: 333 EKTNISSFPYNNASGSSNNMTDLGTLHVSRYGPSLLEALSEIRFNGLAG--RFNLIDRQL 392

Query: 121 LIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMR 180
              ESP F+II  V +  + V FWT   G V  +V  NK  +    + G +     S + 
Sbjct: 393 ---ESPKFEIINFVGNEERIVGFWTPSNGLV--NVNSNKTTSFTGERFGPLIWPGKSTIV 452

Query: 181 VSKANSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISA 240
                     +K++  VP +     FV V            G++ID+  A +  +     
Sbjct: 453 PKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDPITNITTPKGYAIDIFEAALKKLPYSVI 512

Query: 241 YDLFPF---NHSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEK 300
              + F   +  YDD++  V +   D  VGD+TI A R  + DFT+ Y  + + M+V  +
Sbjct: 513 PQYYRFESPDDDYDDLVYKVDNGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMMVPVR 572

Query: 301 QEKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKG-----FGNMVWFSV 360
             + K  W F++ + L +W+      + + FV+WL EH+ N + +G      G   WFS 
Sbjct: 573 DNENKNTWVFLKPWGLDLWVTTACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSF 632

Query: 361 SFIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQM 420
           S + + HRE V + LAR V+  W F +LV+T S++A+LTS +T    QP+ ++V+ L + 
Sbjct: 633 STMVFAHREKVVSNLARFVVVVWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKN 692

Query: 421 PDATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFL 480
            D  VG    +F+ ++L     F  SK+K   S ++    L NGSI AAF    +    L
Sbjct: 693 GD-YVGYQHGAFVKDFLIKE-GFNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLRAIL 752

Query: 481 AKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHK-LLESFKC 540
           ++ C  Y     +FK  G GFAF + S L  +VS++I  +T  + +  +E+K  ++   C
Sbjct: 753 SQYCSKYAIVEPTFKTAGFGFAFPRNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQNDC 812

Query: 541 SSSDKENGLG---LGPEPFLNLFIICGSIALLVLMYIMGL 549
              D +  L    L    F  LF+I G  + L L+  + L
Sbjct: 813 --PDPKTALSSNRLSLRSFWGLFLIAGIASFLALLIFVFL 838

BLAST of Bhi04G000328 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 201.4 bits (511), Expect = 2.6e-50
Identity = 157/575 (27.30%), Postives = 272/575 (47.30%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           M+GV+G +++   +K  LK+F  +++K+    +P++    + +IFA++AYD+  A+  A+
Sbjct: 282 MQGVLGVRSHIPKSK-KLKNFRLRWEKM----FPKKGNDEEMNIFALRAYDSITALAMAV 341

Query: 61  E--------------------NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGM 120
           E                    N+G   +S     L + +    F G++G        NG 
Sbjct: 342 EKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALSNVRFNGLAGEFELI---NGQ 401

Query: 121 LIYESPNFKIIKVVDHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMR 180
           L  ES  F +I ++    + +  W    G V  + +     ++   ++G +     S+  
Sbjct: 402 L--ESSVFDVINIIGSEERIIGLWRPSNGIV--NAKSKNTTSVLGERLGPVIWPGKSKDV 461

Query: 181 VSKANSNNGERKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNI--KNI 240
                     + LR  +P +    EFV+        A   +G+ I++  AV+  +    I
Sbjct: 462 PKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKLPYSVI 521

Query: 241 SAYDLF-PFNHSYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEK 300
             Y  F   + +YD+M+  VY   YD  VGD+TI+A R  +VDFT+ Y  + + M+V  K
Sbjct: 522 PKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSMMVPLK 581

Query: 301 QEKWKRRWAFMEAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKG-----FGNMVWFSV 360
               K  W F+  + L +W+      +F+ F++W++EH+ N + +G      G   WF+ 
Sbjct: 582 DN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTSFWFAF 641

Query: 361 SFIFYMHREPVKNGLARLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQM 420
           S + + HRE V + LAR V+  W F +LV+  S++A+LTS  T    QP+V + + L + 
Sbjct: 642 STMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWKDLIKF 701

Query: 421 PDATVGCNSESFIYNYLNSTLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFL 480
            +  +G    +F+   L S   F+ S++K   S  +  +   NG+I A+F    +  + L
Sbjct: 702 -NKNIGYQRGTFVRELLKSQ-GFDESQLKPFGSAVECDELFSNGTITASFDEVAYIKVIL 761

Query: 481 AKNCKGYTKAVSSFKLGGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFK-- 540
           ++N   YT    SFK  G GF F K S L  +VSR+I  +T    +  +E+K  +     
Sbjct: 762 SQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVTQGEEMQHIENKWFKKPNNC 821

Query: 541 --CSSSDKENGLGLGPEPFLNLFIICGSIALLVLM 544
              ++S   N L L    F  LF+I G  + L L+
Sbjct: 822 PDLNTSLSSNHLSL--SSFWGLFLIAGIASFLALL 838

BLAST of Bhi04G000328 vs. NCBI nr
Match: XP_038884568.1 (glutamate receptor 2.5-like [Benincasa hispida])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM
Sbjct: 297 MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 356

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE 120
           ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE
Sbjct: 357 ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE 416

Query: 121 VAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMRVSKANSNNGERKLRFAVPGQ 180
           VAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMRVSKANSNNGERKLRFAVPGQ
Sbjct: 417 VAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMRVSKANSNNGERKLRFAVPGQ 476

Query: 181 GACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYHK 240
           GACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYHK
Sbjct: 477 GACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYHK 536

Query: 241 EYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLIP 300
           EYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLIP
Sbjct: 537 EYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLIP 596

Query: 301 TMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAIL 360
           TMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAIL
Sbjct: 597 TMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAIL 656

Query: 361 VVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSKI 420
           VVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSKI
Sbjct: 657 VVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSKI 716

Query: 421 KKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGSE 480
           KKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGSE
Sbjct: 717 KKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGSE 776

Query: 481 LGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIALL 540
           LGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIALL
Sbjct: 777 LGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIALL 836

Query: 541 VLMYIMGLQFMVSWKS 557
           VLMYIMGLQFMVSWKS
Sbjct: 837 VLMYIMGLQFMVSWKS 852

BLAST of Bhi04G000328 vs. NCBI nr
Match: XP_008444615.1 (PREDICTED: glutamate receptor 2.5-like [Cucumis melo])

HSP 1 Score: 790.8 bits (2041), Expect = 7.6e-225
Identity = 422/564 (74.82%), Postives = 470/564 (83.33%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIG QTYFNDTK S KSFETKFKK YKLEYPQEEEPTK SIFAIQAYDAAHAIT AM
Sbjct: 302 MEGVIGLQTYFNDTKKSFKSFETKFKKFYKLEYPQEEEPTKVSIFAIQAYDAAHAITRAM 361

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSG-MVRFSK---NNNGMLI--YESPNFKIIKVV 120
                ENL SS  EL EKIL+ NF+GVSG MVRFSK   NNN MLI    SP+FKIIKVV
Sbjct: 362 -----ENLRSSDHELMEKILKINFKGVSGIMVRFSKNHNNNNEMLISSQSSPSFKIIKVV 421

Query: 121 DHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMRVSKA-NSNNGERKL 180
           DH YKEVAFWT K GFVEK V ++K    RS          LSEMRVSK+  S+NGERKL
Sbjct: 422 DHTYKEVAFWTPKLGFVEKYVRVSKNYYKRS----------LSEMRVSKSEKSSNGERKL 481

Query: 181 RFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNH-SYDD 240
            F VP QGACQEFVNVSYYSNGT +N +GFS+DV RAVMNNIK+IS+Y+L PF+H SYD 
Sbjct: 482 TFVVPRQGACQEFVNVSYYSNGTVQNITGFSVDVFRAVMNNIKDISSYELHPFHHQSYDR 541

Query: 241 MIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFE 300
           MI AV +K++DGAVGDITILA+RF+ VDFTVAYL TDIVMVVTEKQEKWKR WAFM+AF+
Sbjct: 542 MIDAVSNKKFDGAVGDITILARRFKSVDFTVAYLKTDIVMVVTEKQEKWKRLWAFMDAFQ 601

Query: 301 LPVWLLIPTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVM 360
            PVW+++PTMHIF+SFVIWL E  NN +L+ FGNM+WFSVS IF++HRE V++GL RL++
Sbjct: 602 YPVWIILPTMHIFISFVIWLTEFPNNQDLRSFGNMLWFSVSVIFHVHREQVRSGLTRLML 661

Query: 361 GPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQ-MPDATVGCNSESFIYNYLNS 420
           GPWLF +LVVTTSFSASLTSLMTNSWSQPSV DVETLKQ MP+ATVGCN+ESFIY+YL +
Sbjct: 662 GPWLFTMLVVTTSFSASLTSLMTNSWSQPSVPDVETLKQTMPNATVGCNAESFIYDYLTT 721

Query: 421 TLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGI 480
           TLEF+ S++K M SIDDYP+ALKNGSI AAFFISPHA+IFLAKN KGYTKAVSSFKLGG+
Sbjct: 722 TLEFDKSRVKTMKSIDDYPEALKNGSISAAFFISPHANIFLAKNRKGYTKAVSSFKLGGM 781

Query: 481 GFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLF 540
           GFAF KGSEL  +VSRSIAELTLANNISTME  LL+SF CSS   ENGLGLGPEPFL LF
Sbjct: 782 GFAFPKGSELAMKVSRSIAELTLANNISTMEKNLLDSFTCSSCKIENGLGLGPEPFLGLF 841

Query: 541 IICGSIALLVLMYIMGLQFMVSWK 556
            ICGSIA L LMY MGLQ +VS K
Sbjct: 842 AICGSIAFLALMY-MGLQLLVSHK 849

BLAST of Bhi04G000328 vs. NCBI nr
Match: XP_022962252.1 (glutamate receptor 2.5-like [Cucurbita moschata])

HSP 1 Score: 745.0 bits (1922), Expect = 4.8e-211
Identity = 387/553 (69.98%), Postives = 451/553 (81.56%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIGF+TYFNDTK S KSFETKFKK+Y LEYP+++EP KASIFA++AYD   +I  AM
Sbjct: 288 MEGVIGFRTYFNDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAM 347

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE 120
           + +G  NL SSS++L E ILESNFEG+SGMVRF    NGMLI  SPNF+IIKVVD +YK 
Sbjct: 348 KTLGDNNL-SSSDQLLETILESNFEGLSGMVRF---KNGMLISRSPNFEIIKVVDQSYKP 407

Query: 121 VAFWTGKFGFVEKSVEINKKEALRSNKMGKM-GVRELSEMRVSKANSNNGERKLRFAVPG 180
           VAFWT K GF E  VE NKK ALRSN MG M GVR LSE    +    +GE++L FAVPG
Sbjct: 408 VAFWTPKSGFAESFVENNKKSALRSNNMGNMVGVRHLSESFTRE--DGDGEKQLTFAVPG 467

Query: 181 QGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYH 240
           QGACQEFVNV  + NGT ++FSGFSI V   +MNNIKN+ +Y    FNHSY+DMI AVY 
Sbjct: 468 QGACQEFVNVHTFENGT-EHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYV 527

Query: 241 KEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLI 300
           KEYDGAVGDITILAKRF+ VDFTVAYL TDIVMVV EK E+W++ WAFM+AF+  VW+LI
Sbjct: 528 KEYDGAVGDITILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLI 587

Query: 301 PTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAI 360
           PTMH+F+S +IWLIE +NN+ELKGFGNM+WFSVS IFYM REPVKNGLARLV+GPWLFAI
Sbjct: 588 PTMHLFISSLIWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNGLARLVLGPWLFAI 647

Query: 361 LVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSK 420
            VVT SFSASLTS++T SWSQPSV  VE LK+M +ATVGCN+ESFI NYL  TL+FE S 
Sbjct: 648 FVVTASFSASLTSMLTISWSQPSVTTVEMLKEM-NATVGCNAESFICNYLKDTLQFESSN 707

Query: 421 IKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGS 480
           IK+M S+D+YPKA ++ +IKAAFFISPHAD+FLAKNC+GYTK VSSFKLGGIGFAF KGS
Sbjct: 708 IKRMESLDNYPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSFKLGGIGFAFPKGS 767

Query: 481 ELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIAL 540
           +  A+VS+SIAELTL NNIS ME  LL SF C S+++ N +GLGP PFL LF +CGSIAL
Sbjct: 768 DFAAKVSKSIAELTLQNNISQMEKTLLNSFNCPSANEGNRVGLGPRPFLGLFFVCGSIAL 827

Query: 541 LVLMYIMGLQFMV 553
            VL+YI GLQFM+
Sbjct: 828 FVLLYI-GLQFMI 831

BLAST of Bhi04G000328 vs. NCBI nr
Match: KAG6598369.1 (Glutamate receptor 2.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 743.4 bits (1918), Expect = 1.4e-210
Identity = 387/553 (69.98%), Postives = 452/553 (81.74%), Query Frame = 0

Query: 1    MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
            MEGVIGF+TYFNDTK S KSFETKFKK+Y LEYP+++EP KASIFA++AYD   +I  AM
Sbjct: 1040 MEGVIGFRTYFNDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAM 1099

Query: 61   ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE 120
            + +G  NL SSS++L E ILESNFEG+SGMVRF    NGMLI +SPNF+IIKVVD +YK 
Sbjct: 1100 KTLGDNNL-SSSDQLLETILESNFEGLSGMVRF---KNGMLISQSPNFEIIKVVDQSYKP 1159

Query: 121  VAFWTGKFGFVEKSVEINKKEALRSNKMGKM-GVRELSEMRVSKANSNNGERKLRFAVPG 180
            VAFWT K GF E  VE NKK +LRSN MG M GVR LSE    +    +GE++L FAVPG
Sbjct: 1160 VAFWTPKSGFAESFVENNKKSSLRSNNMGNMVGVRHLSESFTRE--DGDGEKQLTFAVPG 1219

Query: 181  QGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYH 240
            QGACQEFVNV  + NGT ++FSGFSI V   +MNNIKN+ +Y    FNHSY+DMI AVY 
Sbjct: 1220 QGACQEFVNVHTFENGT-EHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYV 1279

Query: 241  KEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLI 300
            KEYDGAVGDITILAKRF+ VDFTVAYL TDIVMVV EK E+W++ WAFM+AF+  VW+LI
Sbjct: 1280 KEYDGAVGDITILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLI 1339

Query: 301  PTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAI 360
            PTMH+F+S +IWLIE +NN+ELKGFGNM+WFSVS IFYM REPVKNGLARLV+GPWLFAI
Sbjct: 1340 PTMHLFISSLIWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNGLARLVLGPWLFAI 1399

Query: 361  LVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSK 420
             VVT SFSASLTS++T SWSQPSV  VE LK+M +ATVGCN+ESFI NYL  TL+FE S 
Sbjct: 1400 FVVTASFSASLTSMLTISWSQPSVTTVEMLKEM-NATVGCNAESFICNYLKDTLQFESSN 1459

Query: 421  IKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGS 480
            IK+M S+D+YPKA ++ +IKAAFFISPHAD+FL KNCK YTK VSSFKLGGIGFAF KGS
Sbjct: 1460 IKRMESLDNYPKAFEDNTIKAAFFISPHADVFLLKNCKRYTKGVSSFKLGGIGFAFPKGS 1519

Query: 481  ELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIAL 540
            +L A+VS+SIAELTL NNIS ME  LL SF+C S+D+ N +GLGP PFL LF +CGSIAL
Sbjct: 1520 DLAAKVSKSIAELTLQNNISQMEKTLLNSFECPSADEGNRVGLGPPPFLGLFSVCGSIAL 1579

Query: 541  LVLMYIMGLQFMV 553
             VL+YI GLQFM+
Sbjct: 1580 FVLLYI-GLQFMI 1583

BLAST of Bhi04G000328 vs. NCBI nr
Match: XP_023546339.1 (glutamate receptor 2.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739.2 bits (1907), Expect = 2.6e-209
Identity = 386/553 (69.80%), Postives = 450/553 (81.37%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIGF+TYF+DTK S KSFETKFKK+Y LEYP+++EP KASIFA++AYD   +I  AM
Sbjct: 294 MEGVIGFRTYFSDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAM 353

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE 120
           + +G  NL SSS++L E ILESNFEG+SGMVRF    NGMLI +SPNF+IIKVVD +YK 
Sbjct: 354 KTLGDNNL-SSSDQLLETILESNFEGLSGMVRF---KNGMLISQSPNFEIIKVVDQSYKP 413

Query: 121 VAFWTGKFGFVEKSVEINKKEALRSNKMGKM-GVRELSEMRVSKANSNNGERKLRFAVPG 180
           VAFWT K GF E  VE NKK ALRSN MG M GVR LSE    +    +GE++L FAVPG
Sbjct: 414 VAFWTPKSGFAESFVENNKKSALRSNNMGNMVGVRHLSESFTRE--DGDGEKQLTFAVPG 473

Query: 181 QGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYH 240
           QGACQEFVNV  + NGT ++FSGFSI V   +MNNIKN+ +Y    FNHSY+DMI AVY 
Sbjct: 474 QGACQEFVNVHRFENGT-EHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYM 533

Query: 241 KEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLI 300
           KEYDGAVGDITILAKRF+ VDFTVAYL TDIVMVV EK E+W++ WAFM+AF+  VW+LI
Sbjct: 534 KEYDGAVGDITILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLI 593

Query: 301 PTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAI 360
           PTMH+F+S +IWLIE +NN+ELKGFGNM+WFSVS IFYM REPVKN LARLV+GPWLFAI
Sbjct: 594 PTMHLFISSLIWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNVLARLVLGPWLFAI 653

Query: 361 LVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSK 420
            VVT SFSASLTS++T SWSQPSV  VE LK+M +ATVGCN+ESFI NYL  TL+FE S 
Sbjct: 654 FVVTASFSASLTSMLTISWSQPSVTTVEMLKEM-NATVGCNAESFICNYLKDTLQFESSN 713

Query: 421 IKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGS 480
           IK+M S+D+YPKA ++ SIKAAFFISPHAD+FL KNCK YTK VSSFKLGGIGFAF KGS
Sbjct: 714 IKRMESLDNYPKAFEDDSIKAAFFISPHADVFLLKNCKRYTKGVSSFKLGGIGFAFPKGS 773

Query: 481 ELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIAL 540
           +L A+VS+SIAELTL NNIS ME  LL SF C S+++ N +GLGP PFL LF +CGSIAL
Sbjct: 774 DLAAKVSKSIAELTLQNNISQMEKTLLNSFNCPSANEGNRVGLGPRPFLGLFFVCGSIAL 833

Query: 541 LVLMYIMGLQFMV 553
            VL+YI GLQFM+
Sbjct: 834 FVLLYI-GLQFMI 837

BLAST of Bhi04G000328 vs. ExPASy TrEMBL
Match: A0A1S3BAP7 (glutamate receptor 2.5-like OS=Cucumis melo OX=3656 GN=LOC103487885 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 3.7e-225
Identity = 422/564 (74.82%), Postives = 470/564 (83.33%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIG QTYFNDTK S KSFETKFKK YKLEYPQEEEPTK SIFAIQAYDAAHAIT AM
Sbjct: 302 MEGVIGLQTYFNDTKKSFKSFETKFKKFYKLEYPQEEEPTKVSIFAIQAYDAAHAITRAM 361

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSG-MVRFSK---NNNGMLI--YESPNFKIIKVV 120
                ENL SS  EL EKIL+ NF+GVSG MVRFSK   NNN MLI    SP+FKIIKVV
Sbjct: 362 -----ENLRSSDHELMEKILKINFKGVSGIMVRFSKNHNNNNEMLISSQSSPSFKIIKVV 421

Query: 121 DHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMRVSKA-NSNNGERKL 180
           DH YKEVAFWT K GFVEK V ++K    RS          LSEMRVSK+  S+NGERKL
Sbjct: 422 DHTYKEVAFWTPKLGFVEKYVRVSKNYYKRS----------LSEMRVSKSEKSSNGERKL 481

Query: 181 RFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNH-SYDD 240
            F VP QGACQEFVNVSYYSNGT +N +GFS+DV RAVMNNIK+IS+Y+L PF+H SYD 
Sbjct: 482 TFVVPRQGACQEFVNVSYYSNGTVQNITGFSVDVFRAVMNNIKDISSYELHPFHHQSYDR 541

Query: 241 MIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFE 300
           MI AV +K++DGAVGDITILA+RF+ VDFTVAYL TDIVMVVTEKQEKWKR WAFM+AF+
Sbjct: 542 MIDAVSNKKFDGAVGDITILARRFKSVDFTVAYLKTDIVMVVTEKQEKWKRLWAFMDAFQ 601

Query: 301 LPVWLLIPTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVM 360
            PVW+++PTMHIF+SFVIWL E  NN +L+ FGNM+WFSVS IF++HRE V++GL RL++
Sbjct: 602 YPVWIILPTMHIFISFVIWLTEFPNNQDLRSFGNMLWFSVSVIFHVHREQVRSGLTRLML 661

Query: 361 GPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQ-MPDATVGCNSESFIYNYLNS 420
           GPWLF +LVVTTSFSASLTSLMTNSWSQPSV DVETLKQ MP+ATVGCN+ESFIY+YL +
Sbjct: 662 GPWLFTMLVVTTSFSASLTSLMTNSWSQPSVPDVETLKQTMPNATVGCNAESFIYDYLTT 721

Query: 421 TLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGI 480
           TLEF+ S++K M SIDDYP+ALKNGSI AAFFISPHA+IFLAKN KGYTKAVSSFKLGG+
Sbjct: 722 TLEFDKSRVKTMKSIDDYPEALKNGSISAAFFISPHANIFLAKNRKGYTKAVSSFKLGGM 781

Query: 481 GFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLF 540
           GFAF KGSEL  +VSRSIAELTLANNISTME  LL+SF CSS   ENGLGLGPEPFL LF
Sbjct: 782 GFAFPKGSELAMKVSRSIAELTLANNISTMEKNLLDSFTCSSCKIENGLGLGPEPFLGLF 841

Query: 541 IICGSIALLVLMYIMGLQFMVSWK 556
            ICGSIA L LMY MGLQ +VS K
Sbjct: 842 AICGSIAFLALMY-MGLQLLVSHK 849

BLAST of Bhi04G000328 vs. ExPASy TrEMBL
Match: A0A6J1HC77 (glutamate receptor 2.5-like OS=Cucurbita moschata OX=3662 GN=LOC111462756 PE=3 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 2.3e-211
Identity = 387/553 (69.98%), Postives = 451/553 (81.56%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIGF+TYFNDTK S KSFETKFKK+Y LEYP+++EP KASIFA++AYD   +I  AM
Sbjct: 288 MEGVIGFRTYFNDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAM 347

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE 120
           + +G  NL SSS++L E ILESNFEG+SGMVRF    NGMLI  SPNF+IIKVVD +YK 
Sbjct: 348 KTLGDNNL-SSSDQLLETILESNFEGLSGMVRF---KNGMLISRSPNFEIIKVVDQSYKP 407

Query: 121 VAFWTGKFGFVEKSVEINKKEALRSNKMGKM-GVRELSEMRVSKANSNNGERKLRFAVPG 180
           VAFWT K GF E  VE NKK ALRSN MG M GVR LSE    +    +GE++L FAVPG
Sbjct: 408 VAFWTPKSGFAESFVENNKKSALRSNNMGNMVGVRHLSESFTRE--DGDGEKQLTFAVPG 467

Query: 181 QGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYH 240
           QGACQEFVNV  + NGT ++FSGFSI V   +MNNIKN+ +Y    FNHSY+DMI AVY 
Sbjct: 468 QGACQEFVNVHTFENGT-EHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYV 527

Query: 241 KEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLI 300
           KEYDGAVGDITILAKRF+ VDFTVAYL TDIVMVV EK E+W++ WAFM+AF+  VW+LI
Sbjct: 528 KEYDGAVGDITILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLI 587

Query: 301 PTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAI 360
           PTMH+F+S +IWLIE +NN+ELKGFGNM+WFSVS IFYM REPVKNGLARLV+GPWLFAI
Sbjct: 588 PTMHLFISSLIWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNGLARLVLGPWLFAI 647

Query: 361 LVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSK 420
            VVT SFSASLTS++T SWSQPSV  VE LK+M +ATVGCN+ESFI NYL  TL+FE S 
Sbjct: 648 FVVTASFSASLTSMLTISWSQPSVTTVEMLKEM-NATVGCNAESFICNYLKDTLQFESSN 707

Query: 421 IKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGS 480
           IK+M S+D+YPKA ++ +IKAAFFISPHAD+FLAKNC+GYTK VSSFKLGGIGFAF KGS
Sbjct: 708 IKRMESLDNYPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSFKLGGIGFAFPKGS 767

Query: 481 ELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIAL 540
           +  A+VS+SIAELTL NNIS ME  LL SF C S+++ N +GLGP PFL LF +CGSIAL
Sbjct: 768 DFAAKVSKSIAELTLQNNISQMEKTLLNSFNCPSANEGNRVGLGPRPFLGLFFVCGSIAL 827

Query: 541 LVLMYIMGLQFMV 553
            VL+YI GLQFM+
Sbjct: 828 FVLLYI-GLQFMI 831

BLAST of Bhi04G000328 vs. ExPASy TrEMBL
Match: A0A6J1K9A2 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111492270 PE=3 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 3.2e-205
Identity = 382/554 (68.95%), Postives = 449/554 (81.05%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIGF+TYFNDTK S KSFET+FKK+Y LEYP++++P KASIFA++AYDA  +I  AM
Sbjct: 288 MEGVIGFRTYFNDTKKSFKSFETRFKKMYNLEYPEDKDPIKASIFAVRAYDAVRSIARAM 347

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKE 120
           E +G  NL SSS++L E ILESNFEG+SGMVRF    NGMLI +SPNF+IIKVVD +YK 
Sbjct: 348 ETLGDNNL-SSSDQLLETILESNFEGLSGMVRF---KNGMLISQSPNFEIIKVVDQSYKP 407

Query: 121 VAFWTGKFGFVEKSVEINKKEALRSNKMGKM-GVRELSEMRVSKANSNNGERKLRFAVPG 180
           VAFWT K GF E   E NKK ALRSN MG M GVR LSE    +    +GE+ L FAVPG
Sbjct: 408 VAFWTPKSGFAESFEENNKKSALRSNNMGSMVGVRHLSESFTRE--DGDGEKHLTFAVPG 467

Query: 181 QGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYH 240
           QGAC EFVN+  + NGT ++FSGFSI V   VM NIKN+ ++    FNHSY+DMI AVY 
Sbjct: 468 QGACHEFVNMHTFENGT-EHFSGFSIAVFNEVMKNIKNMPSFKFARFNHSYNDMIDAVYR 527

Query: 241 KEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLI 300
           KEYDGAVGDITILAKRF+ VDFTVAYL TDIVMVV EK    ++ WAFM+AF+  VW+LI
Sbjct: 528 KEYDGAVGDITILAKRFQQVDFTVAYLKTDIVMVVREKY--GRKLWAFMDAFKPEVWVLI 587

Query: 301 PTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAI 360
           PTMH+F+S +IWLIE +NN+ELKGFGNM+WFSVS IFY  REPVKNGLARLV+GPWLFAI
Sbjct: 588 PTMHLFISSLIWLIERKNNEELKGFGNMLWFSVSLIFYTQREPVKNGLARLVLGPWLFAI 647

Query: 361 LVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSK 420
            +VT SFSASLTS++T SWSQPSV  VE LK+M +ATVGCN+ESFI +YLN +L+FE ++
Sbjct: 648 FMVTASFSASLTSMLTISWSQPSVTTVEMLKEM-NATVGCNAESFICDYLNGSLKFENAR 707

Query: 421 IKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGS 480
           IKKMNSIDDYPKA +NGSI AAFFISPHAD+FL KNCK YTK VSSFKLGGIGFAF KGS
Sbjct: 708 IKKMNSIDDYPKAFENGSITAAFFISPHADVFLVKNCKRYTKGVSSFKLGGIGFAFPKGS 767

Query: 481 ELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLFIICGSIAL 540
           +L A+VS+SIAELTL NNIS ME  LL SF+C S+D+ N +GLG  PF+ LF++CGSIAL
Sbjct: 768 DLAAKVSKSIAELTLQNNISQMEKTLLNSFQCPSADEGNRVGLGLRPFVGLFLVCGSIAL 827

Query: 541 LVLMYIMGLQFMVS 554
            VL+YI GLQFM++
Sbjct: 828 FVLLYI-GLQFMIA 830

BLAST of Bhi04G000328 vs. ExPASy TrEMBL
Match: A0A0A0LRB3 (PBPe domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G357240 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 7.2e-205
Identity = 387/557 (69.48%), Postives = 445/557 (79.89%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIG QTY NDTK S KSFETKFKK YKLEYPQEEEPTKASIFAIQAYDAA AIT AM
Sbjct: 204 MEGVIGLQTYVNDTKKSFKSFETKFKKFYKLEYPQEEEPTKASIFAIQAYDAAQAITRAM 263

Query: 61  ENMGVENLSSSSEELKEKILESNFEGVSG-MVRFSK---NNNGMLIYES--PNFKIIKVV 120
                ENL SS  EL EKIL+SNF+GVSG MVRFSK   NNNGMLI     PNFKIIKVV
Sbjct: 264 -----ENLRSSDHELMEKILKSNFKGVSGIMVRFSKNNTNNNGMLISSQSLPNFKIIKVV 323

Query: 121 DHAYKEVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMRVSKA-NSNNGERKL 180
           +  YKEVAFWT K GFVEK VE++K             +R LSEMR  ++  S N +RKL
Sbjct: 324 NQTYKEVAFWTPKLGFVEKFVEVSKNY-----------MRSLSEMRAPESEQSRNRQRKL 383

Query: 181 RFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIK-NISAYDLFPFNH-SYD 240
           +F VPGQGACQEFVNVSYY NGT +N +GFS+DV RAVMNNI  + S+Y + PF+H SYD
Sbjct: 384 KFVVPGQGACQEFVNVSYYPNGTIQNITGFSVDVFRAVMNNINYDTSSYWMHPFHHRSYD 443

Query: 241 DMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAF 300
            MI AV  K+YDGA+GDITILA+RF  VDFTVAYL TDIVM+VTEKQE  K+ WAFM AF
Sbjct: 444 SMIDAVSQKKYDGAIGDITILARRFNSVDFTVAYLKTDIVMIVTEKQETLKKVWAFMGAF 503

Query: 301 ELPVWLLIPTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLV 360
           + PVW+++PTMHIF+ F++WLIE+ NN +L+ FGNM+WFSV+ IF++HRE V+NGLARLV
Sbjct: 504 QRPVWIILPTMHIFICFLVWLIEYPNNLDLRSFGNMLWFSVTVIFHVHREQVRNGLARLV 563

Query: 361 MGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNS 420
           +G WLFA+LV T SFSASL SL   SWSQPSV++VE LKQMP A VGCN+ESFIY+YL +
Sbjct: 564 LGSWLFALLVATNSFSASLISLTAYSWSQPSVVNVEMLKQMPYAKVGCNAESFIYDYLTT 623

Query: 421 TLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGI 480
           TLEF+ S++K MNSIDDY +ALKN SI AAFFISPHA+IFLAKN KGYTKAVSSFKLGG+
Sbjct: 624 TLEFDKSRVKTMNSIDDYAEALKNRSINAAFFISPHANIFLAKNRKGYTKAVSSFKLGGM 683

Query: 481 GFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFLNLF 540
           GFAF KGSEL  +VS SIAELTLANNISTME  LL+SF CSS ++++G GLGPEPFL LF
Sbjct: 684 GFAFPKGSELATKVSSSIAELTLANNISTMEKNLLDSFTCSSCERDSGPGLGPEPFLALF 743

Query: 541 IICGSIALLVLMYIMGL 549
            ICGSIA   L+ I+ L
Sbjct: 744 AICGSIAFFALIKIVPL 744

BLAST of Bhi04G000328 vs. ExPASy TrEMBL
Match: A0A5A7V226 (Glutamate receptor 2.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00400 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 8.6e-198
Identity = 373/563 (66.25%), Postives = 441/563 (78.33%), Query Frame = 0

Query: 1   MEGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAM 60
           MEGVIG +TYFND+K S KSFETKFKKIYKLEYPQ+EEPTKASIFAI+AYDAA A+T AM
Sbjct: 142 MEGVIGLETYFNDSKKSFKSFETKFKKIYKLEYPQDEEPTKASIFAIRAYDAARAVTRAM 201

Query: 61  ENMGVE-NLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYK 120
           E +G +  L SSS++L+EKILESNFEGV GMV+FSK  NGMLI ESPNFKI+KVVD  YK
Sbjct: 202 EKLGDDKKLRSSSKQLREKILESNFEGVGGMVKFSK-QNGMLISESPNFKIVKVVDQTYK 261

Query: 121 EVAFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRE------LSEMRVSKA---NSNNGE 180
           EV FWT   GFVE  VEI  K      K  K  +R+      LS ++ S +   ++++G+
Sbjct: 262 EVGFWTPNLGFVENYVEIINKTTTELVKHNKGNLRKIVSVGNLSRLKTSSSENFDNDHGK 321

Query: 181 RKLRFAVPGQGACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNI--KNISAYDLFPFNH 240
           ++L FAVP + AC+E V VS + NG     +GFSI++ RAVMNNI   + S+Y+L PF  
Sbjct: 322 KRLTFAVPEEAACKELVKVSQHLNG--DYITGFSINLFRAVMNNINTSDFSSYELVPFKG 381

Query: 241 SYDDMIKAVYHKEYDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFM 300
            YDDMIKAV +K Y GAVGDI ILA R+++VDFTV+YL T+IVMVV +K +KWK+ WAFM
Sbjct: 382 RYDDMIKAVSNKTYFGAVGDIIILAHRYKYVDFTVSYLQTEIVMVVEQKDDKWKKIWAFM 441

Query: 301 EAFELPVWLLIPTMHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLA 360
           EAFEL +WLLIPTMH+F+SFVIWLIE QNN EL+G GNM+WFS+S IFYMHREP+KNGLA
Sbjct: 442 EAFELTMWLLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSISIIFYMHREPLKNGLA 501

Query: 361 RLVMGPWLFAILVVTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNY 420
           RLV+GPWLFAILVVT SF+ASLTS+MT SW +PSV DVETLKQM    VGCN+ SFI NY
Sbjct: 502 RLVLGPWLFAILVVTASFTASLTSMMTISWFRPSVPDVETLKQM-GYNVGCNTNSFICNY 561

Query: 421 LNSTLEFEPSKIKKMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKL 480
           L  TLEF+P+KIKK++SID+YPKA KNG+IKAAFFISPHA ++LAK CKGYTK VSSFKL
Sbjct: 562 LVDTLEFDPTKIKKIDSIDEYPKAFKNGTIKAAFFISPHAKVYLAKYCKGYTKGVSSFKL 621

Query: 481 GGIGFAFRKGSELGAEVSRSIAELTLANNISTMEHKLLESFKCSSSDKENGLGLGPEPFL 540
            GIGFA  KGSEL + VS SI ELT    I   E  +L SF CSS+ K +GLGLGPEPF+
Sbjct: 622 SGIGFAMAKGSELASRVSASIVELTETKEIPQFESDVLASFNCSSNGKGDGLGLGPEPFM 681

Query: 541 NLFIICGSIALLVLMYIMGLQFM 552
            LFIICGSIA LVL+Y M  QFM
Sbjct: 682 GLFIICGSIAFLVLIY-MAQQFM 699