Bhi04G000326 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G000326
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Glutamate receptor
Location	chr4: 8574042 .. 8574326 (-)
RNA-Seq Expression	Bhi04G000326
Synteny	Bhi04G000326

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATTTCCAAAAGGGTCTCCCTTGACGGTGGAAATATCGGCAGCAATCGCGGAGTTGACTGAAAGAAGGGAAATTCCGGATTTGAAGACCACATTGCTTTCAACCTTCAACTGCTCCTCAAACGACGGTGATACAGATGGGTCGGGTTTAGGACCCGAACCTTTTGTCGGTTTGCTTCTCATTTCTGGATTTATAGCTTTCATGGCTATTCTAATCACTACTTCTCGTCTTGTATTCTTCAAGTTTGGCTGCATCACTTCAACCAAACCCCATATCCCCATTTAG

mRNA sequence

GCATTTCCAAAAGGGTCTCCCTTGACGGTGGAAATATCGGCAGCAATCGCGGAGTTGACTGAAAGAAGGGAAATTCCGGATTTGAAGACCACATTGCTTTCAACCTTCAACTGCTCCTCAAACGACGGTGATACAGATGGGTCGGGTTTAGGACCCGAACCTTTTGTCGGTTTGCTTCTCATTTCTGGATTTATAGCTTTCATGGCTATTCTAATCACTACTTCTCGTCTTGTATTCTTCAAGTTTGGCTGCATCACTTCAACCAAACCCCATATCCCCATTTAG

Coding sequence (CDS)

GCATTTCCAAAAGGGTCTCCCTTGACGGTGGAAATATCGGCAGCAATCGCGGAGTTGACTGAAAGAAGGGAAATTCCGGATTTGAAGACCACATTGCTTTCAACCTTCAACTGCTCCTCAAACGACGGTGATACAGATGGGTCGGGTTTAGGACCCGAACCTTTTGTCGGTTTGCTTCTCATTTCTGGATTTATAGCTTTCATGGCTATTCTAATCACTACTTCTCGTCTTGTATTCTTCAAGTTTGGCTGCATCACTTCAACCAAACCCCATATCCCCATTTAG

Protein sequence

AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLLISGFIAFMAILITTSRLVFFKFGCITSTKPHIPI

Homology

[Close]

BLAST of Bhi04G000326 vs. TAIR 10
Match: AT4G35290.2 (glutamate receptor 2 )

HSP 1 Score: 49.3 bits (116), Expect = 2.0e-06
Identity = 32/88 (36.36%), Postives = 48/88 (54.55%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDG---DTDGSGLGPEPFVG 60
           AFP+ SPL +++S AI  L+E  ++  +    LS  NCS+ +G   D D   L    F G
Sbjct: 772 AFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLSRSNCSNLNGSVSDEDSEQLKLRSFWG 831

Query: 61  LLLISGFIAFMAILITTSRLV--FFKFG 84
           L L+ G   F+A+ I   ++V  FF+ G
Sbjct: 832 LFLVCGISCFIALFIYFFKIVRDFFRHG 859

[Close]

BLAST of Bhi04G000326 vs. TAIR 10
Match: AT4G35290.1 (glutamate receptor 2 )

HSP 1 Score: 49.3 bits (116), Expect = 2.0e-06
Identity = 32/88 (36.36%), Postives = 48/88 (54.55%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDG---DTDGSGLGPEPFVG 60
           AFP+ SPL +++S AI  L+E  ++  +    LS  NCS+ +G   D D   L    F G
Sbjct: 772 AFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLSRSNCSNLNGSVSDEDSEQLKLRSFWG 831

Query: 61  LLLISGFIAFMAILITTSRLV--FFKFG 84
           L L+ G   F+A+ I   ++V  FF+ G
Sbjct: 832 LFLVCGISCFIALFIYFFKIVRDFFRHG 859

[Close]

BLAST of Bhi04G000326 vs. TAIR 10
Match: AT2G17260.1 (glutamate receptor 2 )

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-05
Identity = 28/80 (35.00%), Postives = 41/80 (51.25%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSG--LGPEPFVGL 60
           AFP+ SPL V++S AI  L+E  E+  +    LS  NCSS  G   G    L    F G+
Sbjct: 802 AFPRDSPLAVDMSTAILGLSETGELQKIHDRWLSKSNCSSPHGSQSGDSEQLNVHSFWGM 861

Query: 61  LLISGFIAFMAILITTSRLV 79
            L+ G    +A+ I   +++
Sbjct: 862 FLVVGIACLVALFIHFFKII 881

[Close]

BLAST of Bhi04G000326 vs. TAIR 10
Match: AT1G05200.1 (glutamate receptor 3.4 )

HSP 1 Score: 42.7 bits (99), Expect = 1.8e-04
Identity = 29/83 (34.94%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLS-TFNCSSNDGDTDGSGLGPEPFVGLL 60
           AF + SPL V++S AI +L+E  E+  +    L+    CS    +++ S L  + F GL 
Sbjct: 803 AFQRDSPLAVDMSTAILQLSEEGELEKIHRKWLNYKHECSMQISNSEDSQLSLKSFWGLF 862

Query: 61  LISGFIAFMAILITTSRLVFFKF 83
           LI G   FMA+ +   R VF+++
Sbjct: 863 LICGITCFMALTVFFWR-VFWQY 884

[Close]

BLAST of Bhi04G000326 vs. TAIR 10
Match: AT1G05200.2 (glutamate receptor 3.4 )

HSP 1 Score: 42.7 bits (99), Expect = 1.8e-04
Identity = 29/83 (34.94%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLS-TFNCSSNDGDTDGSGLGPEPFVGLL 60
           AF + SPL V++S AI +L+E  E+  +    L+    CS    +++ S L  + F GL 
Sbjct: 803 AFQRDSPLAVDMSTAILQLSEEGELEKIHRKWLNYKHECSMQISNSEDSQLSLKSFWGLF 862

Query: 61  LISGFIAFMAILITTSRLVFFKF 83
           LI G   FMA+ +   R VF+++
Sbjct: 863 LICGITCFMALTVFFWR-VFWQY 884

[Close]

BLAST of Bhi04G000326 vs. ExPASy Swiss-Prot
Match: Q93YT1 (Glutamate receptor 3.2 OS=Arabidopsis thaliana OX=3702 GN=GLR3.2 PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 2.8e-05
Identity = 32/88 (36.36%), Postives = 48/88 (54.55%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDG---DTDGSGLGPEPFVG 60
           AFP+ SPL +++S AI  L+E  ++  +    LS  NCS+ +G   D D   L    F G
Sbjct: 772 AFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLSRSNCSNLNGSVSDEDSEQLKLRSFWG 831

Query: 61  LLLISGFIAFMAILITTSRLV--FFKFG 84
           L L+ G   F+A+ I   ++V  FF+ G
Sbjct: 832 LFLVCGISCFIALFIYFFKIVRDFFRHG 859

[Close]

BLAST of Bhi04G000326 vs. ExPASy Swiss-Prot
Match: Q7XJL2 (Glutamate receptor 3.1 OS=Arabidopsis thaliana OX=3702 GN=GLR3.1 PE=2 SV=3)

HSP 1 Score: 46.6 bits (109), Expect = 1.8e-04
Identity = 28/80 (35.00%), Postives = 41/80 (51.25%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSG--LGPEPFVGL 60
           AFP+ SPL V++S AI  L+E  E+  +    LS  NCSS  G   G    L    F G+
Sbjct: 776 AFPRDSPLAVDMSTAILGLSETGELQKIHDRWLSKSNCSSPHGSQSGDSEQLNVHSFWGM 835

Query: 61  LLISGFIAFMAILITTSRLV 79
            L+ G    +A+ I   +++
Sbjct: 836 FLVVGIACLVALFIHFFKII 855

[Close]

BLAST of Bhi04G000326 vs. NCBI nr
Match: XP_022997326.1 (LOW QUALITY PROTEIN: glutamate receptor 2.7-like [Cucurbita maxima])

HSP 1 Score: 137.5 bits (345), Expect = 5.9e-29
Identity = 74/94 (78.72%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
           AFPKGSPLTV++SA+IAEL ERREIPDLKTTLLSTFNCSSN  D DG GLGPEPFVGLLL
Sbjct: 744 AFPKGSPLTVDVSASIAELIERREIPDLKTTLLSTFNCSSNYDDPDGPGLGPEPFVGLLL 803

Query: 61  ISGFIAFMAILITTSRLVFFKFGCITSTKPHIPI 95
           IS  IA MA+LIT  RLVFF     T TKPHIPI
Sbjct: 804 ISCLIASMAVLITVCRLVFFN---PTPTKPHIPI 834

[Close]

BLAST of Bhi04G000326 vs. NCBI nr
Match: XP_022144363.1 (glutamate receptor 2.9-like [Momordica charantia])

HSP 1 Score: 125.2 bits (313), Expect = 3.0e-25
Identity = 64/97 (65.98%), Postives = 74/97 (76.29%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
           AFPKGSPLTV+ISA+IAEL ERRE+PDL++TLLSTFNCS ND + DGSGLGPEPF GL L
Sbjct: 747 AFPKGSPLTVDISASIAELIERRELPDLESTLLSTFNCSLNDNNVDGSGLGPEPFAGLFL 806

Query: 61  ISGFIAFMAILITTSRLVFFKFGCI----TSTKPHIP 94
           I+G I   A+L T  RL   K G I    T+TKP +P
Sbjct: 807 IAGAITLAAVLFTAGRLTLMKLGWIKDEPTTTKPQLP 843

[Close]

BLAST of Bhi04G000326 vs. NCBI nr
Match: KGN62744.2 (hypothetical protein Csa_018680 [Cucumis sativus])

HSP 1 Score: 123.6 bits (309), Expect = 8.9e-25
Identity = 66/99 (66.67%), Postives = 73/99 (73.74%), Query Frame = 0


Query: 1    AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
            AFPKGSPLTV+ISA+IAELTERRE+PDL++TLLSTFNCS ND D DGS LGPEPF GL L
Sbjct: 1584 AFPKGSPLTVDISASIAELTERREMPDLESTLLSTFNCSLNDNDPDGSALGPEPFAGLFL 1643

Query: 61   ISGFIAFMAILITTSRLVFFKFGCI-----TSTKPHIPI 95
            ISG IA  A+L T  RL+    G I     T  K H PI
Sbjct: 1644 ISGSIALGALLFTAGRLILRSLGWIKQHPATKPKSHFPI 1682

[Close]

BLAST of Bhi04G000326 vs. NCBI nr
Match: XP_038885764.1 (glutamate receptor 2.5-like [Benincasa hispida])

HSP 1 Score: 123.2 bits (308), Expect = 1.2e-24
Identity = 62/85 (72.94%), Postives = 71/85 (83.53%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
           AFPKGSPLTV+ISA+IAEL ERRE+PDL++TLLSTFNCS ND D DGSGLGPEPF GL L
Sbjct: 750 AFPKGSPLTVDISASIAELIERREMPDLESTLLSTFNCSLNDNDPDGSGLGPEPFAGLFL 809

Query: 61  ISGFIAFMAILITTSRLVFFKFGCI 86
           I+G IAF A+L T +RL+  K G I
Sbjct: 810 IAGAIAFGALLFTAARLILMKLGWI 834

[Close]

BLAST of Bhi04G000326 vs. NCBI nr
Match: KAA0060906.1 (glutamate receptor 2.5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 120.6 bits (301), Expect = 7.5e-24
Identity = 63/98 (64.29%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 1    AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
            AFPKGSPLTV+ISA+IAEL ERRE+PDL++TLLSTFNCS ND D DGS LGPEPF GL L
Sbjct: 2703 AFPKGSPLTVDISASIAELIERREMPDLESTLLSTFNCSLNDNDPDGSALGPEPFAGLFL 2762

Query: 61   ISGFIAFMAILITTSRLVFFKFGCI----TSTKPHIPI 95
            I+G IA  A++ T  RL+    G I    T  K H PI
Sbjct: 2763 IAGSIALGALVFTVGRLILLNLGWIKQNPTKPKSHFPI 2800

[Close]

BLAST of Bhi04G000326 vs. ExPASy TrEMBL
Match: A0A6J1K770 (LOW QUALITY PROTEIN: glutamate receptor 2.7-like OS=Cucurbita maxima OX=3661 GN=LOC111492271 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.9e-29
Identity = 74/94 (78.72%), Postives = 78/94 (82.98%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
           AFPKGSPLTV++SA+IAEL ERREIPDLKTTLLSTFNCSSN  D DG GLGPEPFVGLLL
Sbjct: 744 AFPKGSPLTVDVSASIAELIERREIPDLKTTLLSTFNCSSNYDDPDGPGLGPEPFVGLLL 803

Query: 61  ISGFIAFMAILITTSRLVFFKFGCITSTKPHIPI 95
           IS  IA MA+LIT  RLVFF     T TKPHIPI
Sbjct: 804 ISCLIASMAVLITVCRLVFFN---PTPTKPHIPI 834

[Close]

BLAST of Bhi04G000326 vs. ExPASy TrEMBL
Match: A0A6J1CT28 (Glutamate receptor OS=Momordica charantia OX=3673 GN=LOC111014067 PE=3 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-25
Identity = 64/97 (65.98%), Postives = 74/97 (76.29%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
           AFPKGSPLTV+ISA+IAEL ERRE+PDL++TLLSTFNCS ND + DGSGLGPEPF GL L
Sbjct: 747 AFPKGSPLTVDISASIAELIERRELPDLESTLLSTFNCSLNDNNVDGSGLGPEPFAGLFL 806

Query: 61  ISGFIAFMAILITTSRLVFFKFGCI----TSTKPHIP 94
           I+G I   A+L T  RL   K G I    T+TKP +P
Sbjct: 807 IAGAITLAAVLFTAGRLTLMKLGWIKDEPTTTKPQLP 843

[Close]

BLAST of Bhi04G000326 vs. ExPASy TrEMBL
Match: A0A0A0LL89 (PBPe domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G357260 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.3e-25
Identity = 66/99 (66.67%), Postives = 73/99 (73.74%), Query Frame = 0


Query: 1    AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
            AFPKGSPLTV+ISA+IAELTERRE+PDL++TLLSTFNCS ND D DGS LGPEPF GL L
Sbjct: 1396 AFPKGSPLTVDISASIAELTERREMPDLESTLLSTFNCSLNDNDPDGSALGPEPFAGLFL 1455

Query: 61   ISGFIAFMAILITTSRLVFFKFGCI-----TSTKPHIPI 95
            ISG IA  A+L T  RL+    G I     T  K H PI
Sbjct: 1456 ISGSIALGALLFTAGRLILRSLGWIKQHPATKPKSHFPI 1494

[Close]

BLAST of Bhi04G000326 vs. ExPASy TrEMBL
Match: A0A5A7V316 (Glutamate receptor 2.5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00390 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.6e-24
Identity = 63/98 (64.29%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 1    AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
            AFPKGSPLTV+ISA+IAEL ERRE+PDL++TLLSTFNCS ND D DGS LGPEPF GL L
Sbjct: 2703 AFPKGSPLTVDISASIAELIERREMPDLESTLLSTFNCSLNDNDPDGSALGPEPFAGLFL 2762

Query: 61   ISGFIAFMAILITTSRLVFFKFGCI----TSTKPHIPI 95
            I+G IA  A++ T  RL+    G I    T  K H PI
Sbjct: 2763 IAGSIALGALVFTVGRLILLNLGWIKQNPTKPKSHFPI 2800

[Close]

BLAST of Bhi04G000326 vs. ExPASy TrEMBL
Match: A0A1S3BBH6 (Glutamate receptor OS=Cucumis melo OX=3656 GN=LOC103487888 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.6e-24
Identity = 63/98 (64.29%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 1   AFPKGSPLTVEISAAIAELTERREIPDLKTTLLSTFNCSSNDGDTDGSGLGPEPFVGLLL 60
           AFPKGSPLTV+ISA+IAEL ERRE+PDL++TLLSTFNCS ND D DGS LGPEPF GL L
Sbjct: 753 AFPKGSPLTVDISASIAELIERREMPDLESTLLSTFNCSLNDNDPDGSALGPEPFAGLFL 812

Query: 61  ISGFIAFMAILITTSRLVFFKFGCI----TSTKPHIPI 95
           I+G IA  A++ T  RL+    G I    T  K H PI
Sbjct: 813 IAGSIALGALVFTVGRLILLNLGWIKQNPTKPKSHFPI 850

The following BLAST results are available for this feature:

BLAST of Bhi04G000326 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35290.2	2.0e-06	36.36	glutamate receptor 2	[more]
AT4G35290.1	2.0e-06	36.36	glutamate receptor 2	[more]
AT2G17260.1	1.3e-05	35.00	glutamate receptor 2	[more]
AT1G05200.1	1.8e-04	34.94	glutamate receptor 3.4	[more]
AT1G05200.2	1.8e-04	34.94	glutamate receptor 3.4	[more]

BLAST of Bhi04G000326 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93YT1	2.8e-05	36.36	Glutamate receptor 3.2 OS=Arabidopsis thaliana OX=3702 GN=GLR3.2 PE=1 SV=2	[more]
Q7XJL2	1.8e-04	35.00	Glutamate receptor 3.1 OS=Arabidopsis thaliana OX=3702 GN=GLR3.1 PE=2 SV=3	[more]

BLAST of Bhi04G000326 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022997326.1	5.9e-29	78.72	LOW QUALITY PROTEIN: glutamate receptor 2.7-like [Cucurbita maxima]	[more]
XP_022144363.1	3.0e-25	65.98	glutamate receptor 2.9-like [Momordica charantia]	[more]
KGN62744.2	8.9e-25	66.67	hypothetical protein Csa_018680 [Cucumis sativus]	[more]
XP_038885764.1	1.2e-24	72.94	glutamate receptor 2.5-like [Benincasa hispida]	[more]
KAA0060906.1	7.5e-24	64.29	glutamate receptor 2.5-like isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of Bhi04G000326 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K770	2.9e-29	78.72	LOW QUALITY PROTEIN: glutamate receptor 2.7-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1CT28	1.5e-25	65.98	Glutamate receptor OS=Momordica charantia OX=3673 GN=LOC111014067 PE=3 SV=1	[more]
A0A0A0LL89	4.3e-25	66.67	PBPe domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G357260 PE=3 S...	[more]
A0A5A7V316	3.6e-24	64.29	Glutamate receptor 2.5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BBH6	3.6e-24	64.29	Glutamate receptor OS=Cucumis melo OX=3656 GN=LOC103487888 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR18966:SF401	GLUTAMATE RECEPTOR	coord: 1..80
None	No IPR available	PANTHER	PTHR18966	IONOTROPIC GLUTAMATE RECEPTOR	coord: 1..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M000326	Bhi04M000326	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034220	ion transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015276	ligand-gated ion channel activity
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0038023	signaling receptor activity