Bhi04G000303 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G000303
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	calmodulin-like
Location	chr4: 7755465 .. 7755776 (-)
RNA-Seq Expression	Bhi04G000303
Synteny	Bhi04G000303

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACCAGAAAAAGCAGTTGCGAGACAAAATTCAAAATTCATCAACAAAAGTTCTGGATAGAAAGCAATCTAATATTCCTCTTAGTCGAGAGAGGATAAAGGAGATTTTTAAATATCATGATAGCAACAAAGATGGTTTTTTCAGCATTCGGGAGTTAGCAAAAACTTTCGACTCTCTAGGCTCAGTTATTCCATTCTACAAAGCTCACTATGCTATGGCTTATGCAGATGCAGATAAAGATGGTCTTATCAGTGAGGCCGAACTTGACAAACTCATCGATTATACCAATAAAATCATCAAGAAGAAATGA

mRNA sequence

ATGAACCAGAAAAAGCAGTTGCGAGACAAAATTCAAAATTCATCAACAAAAGTTCTGGATAGAAAGCAATCTAATATTCCTCTTAGTCGAGAGAGGATAAAGGAGATTTTTAAATATCATGATAGCAACAAAGATGGTTTTTTCAGCATTCGGGAGTTAGCAAAAACTTTCGACTCTCTAGGCTCAGTTATTCCATTCTACAAAGCTCACTATGCTATGGCTTATGCAGATGCAGATAAAGATGGTCTTATCAGTGAGGCCGAACTTGACAAACTCATCGATTATACCAATAAAATCATCAAGAAGAAATGA

Coding sequence (CDS)

ATGAACCAGAAAAAGCAGTTGCGAGACAAAATTCAAAATTCATCAACAAAAGTTCTGGATAGAAAGCAATCTAATATTCCTCTTAGTCGAGAGAGGATAAAGGAGATTTTTAAATATCATGATAGCAACAAAGATGGTTTTTTCAGCATTCGGGAGTTAGCAAAAACTTTCGACTCTCTAGGCTCAGTTATTCCATTCTACAAAGCTCACTATGCTATGGCTTATGCAGATGCAGATAAAGATGGTCTTATCAGTGAGGCCGAACTTGACAAACTCATCGATTATACCAATAAAATCATCAAGAAGAAATGA

Protein sequence

MNQKKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSVIPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK

Homology

[Close]

BLAST of Bhi04G000303 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 47.4 bits (111), Expect = 8.2e-06
Identity = 29/98 (29.59%), Postives = 52/98 (53.06%), Query Frame = 0


Query: 5   KQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSVI 64
           ++L  K ++S+ ++     SN       ++ +F Y D+N+DG  S  EL K+F +LG  +
Sbjct: 16  RKLSPKREDSAGEIQQHNSSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQL 75

Query: 65  PFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKK 103
              +A  A+  +D D DG++   E  +LI   ++  KK
Sbjct: 76  SDEEAVAAVRLSDTDGDGMLDFEEFSQLIKVDDEEEKK 113

[Close]

BLAST of Bhi04G000303 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 47.4 bits (111), Expect = 8.2e-06
Identity = 29/98 (29.59%), Postives = 52/98 (53.06%), Query Frame = 0


Query: 5   KQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSVI 64
           ++L  K ++S+ ++     SN       ++ +F Y D+N+DG  S  EL K+F +LG  +
Sbjct: 16  RKLSPKREDSAGEIQQHNSSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQL 75

Query: 65  PFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKK 103
              +A  A+  +D D DG++   E  +LI   ++  KK
Sbjct: 76  SDEEAVAAVRLSDTDGDGMLDFEEFSQLIKVDDEEEKK 113

[Close]

BLAST of Bhi04G000303 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 42.0 bits (97), Expect = 3.5e-04
Identity = 30/103 (29.13%), Postives = 53/103 (51.46%), Query Frame = 0


Query: 5   KQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSVI 64
           K  + ++ +S  K L+ K  +       ++ +F Y D+N+DG  S  EL K+F +LG  +
Sbjct: 2   KNTQRQLSSSFMKFLEEKNRD-------LEAVFAYMDANRDGRISAEELKKSFKTLGEQM 61

Query: 65  PFYKAHYAMAYADADKDGLISEAELDKLI----DYTNKIIKKK 104
              +A  A+  +D D DG++   E   LI    ++T +  K+K
Sbjct: 62  SDEEAEAAVKLSDIDGDGMLDINEFALLIKGNDEFTEEEKKRK 97

[Close]

BLAST of Bhi04G000303 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.2e-04
Identity = 29/98 (29.59%), Postives = 52/98 (53.06%), Query Frame = 0


Query: 5   KQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSVI 64
           ++L  K ++S+ ++     SN       ++ +F Y D+N+DG  S  EL K+F +LG  +
Sbjct: 16  RKLSPKREDSAGEIQQHNSSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQL 75

Query: 65  PFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKK 103
              +A  A+  +D D DG++   E  +LI   ++  KK
Sbjct: 76  SDEEAVAAVRLSDTDGDGMLDFEEFSQLIKVDDEEEKK 113

[Close]

BLAST of Bhi04G000303 vs. NCBI nr
Match: XP_038886759.1 (probable calcium-binding protein CML11 [Benincasa hispida])

HSP 1 Score: 183.7 bits (465), Expect = 7.9e-43
Identity = 94/103 (91.26%), Postives = 97/103 (94.17%), Query Frame = 0


Query: 1   MNQKKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSL 60
           MNQKKQLRDKIQNSSTK LDRKQSNIP SRE+IKEIFKYHDSNKDGF +IREL KTF SL
Sbjct: 75  MNQKKQLRDKIQNSSTKALDRKQSNIPPSREQIKEIFKYHDSNKDGFLNIRELTKTFGSL 134

Query: 61  GSVIPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           GSVIPFYKAHYAMAYADADKDGLISE ELD+LIDYTNKIIKKK
Sbjct: 135 GSVIPFYKAHYAMAYADADKDGLISEVELDELIDYTNKIIKKK 177

[Close]

BLAST of Bhi04G000303 vs. NCBI nr
Match: XP_004152801.1 (probable calcium-binding protein CML11 [Cucumis sativus] >KGN62526.1 hypothetical protein Csa_021903 [Cucumis sativus])

HSP 1 Score: 145.2 bits (365), Expect = 3.1e-31
Identity = 75/100 (75.00%), Postives = 83/100 (83.00%), Query Frame = 0


Query: 4   KKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSV 63
           KKQL DK Q SS      KQSN+PLSRE+IKEIFKYHDSNKDGF SIREL K F S+GS+
Sbjct: 78  KKQLLDKAQGSS------KQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMGSI 137

Query: 64  IPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           +PF KA YAMAYAD D+DGL+SEAELDKLIDY +KIIKKK
Sbjct: 138 VPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]

BLAST of Bhi04G000303 vs. NCBI nr
Match: XP_008445564.1 (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo] >KAA0060885.1 putative calcium-binding protein CML11 [Cucumis melo var. makuwa])

HSP 1 Score: 137.5 bits (345), Expect = 6.5e-29
Identity = 73/100 (73.00%), Postives = 81/100 (81.00%), Query Frame = 0


Query: 4   KKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSV 63
           KKQL DK Q+S       +QSN+PLSRE+IKEIFKYHDSNKDGF SIREL K F SLGS+
Sbjct: 74  KKQLLDKAQDS------LRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGSI 133

Query: 64  IPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           +PF KA +AMAYAD D+DGLISEAEL KLIDY  KIIKKK
Sbjct: 134 VPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

[Close]

BLAST of Bhi04G000303 vs. NCBI nr
Match: XP_022951799.1 (probable calcium-binding protein CML11 [Cucurbita moschata])

HSP 1 Score: 135.6 bits (340), Expect = 2.5e-28
Identity = 69/102 (67.65%), Postives = 83/102 (81.37%), Query Frame = 0


Query: 2   NQKKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLG 61
           NQK Q++ K+  SSTKVL  K SN+PLSRE+IK+IFKY+DS+KDGF +IRE+ K F  LG
Sbjct: 72  NQKIQVQGKVTKSSTKVLSEKISNVPLSREQIKKIFKYYDSDKDGFLNIREVTKAFSFLG 131

Query: 62  SVIPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           S+ PFYKA YAM +ADA+ DGLISE EL+KLIDY  KIIKKK
Sbjct: 132 SINPFYKAQYAMTFADANNDGLISEVELEKLIDYACKIIKKK 173

[Close]

BLAST of Bhi04G000303 vs. NCBI nr
Match: XP_022951742.1 (probable calcium-binding protein CML11 [Cucurbita moschata])

HSP 1 Score: 134.4 bits (337), Expect = 5.5e-28
Identity = 69/102 (67.65%), Postives = 82/102 (80.39%), Query Frame = 0


Query: 2   NQKKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLG 61
           N K Q++ K   SSTKVL  K SN+PLSRE+IK+IFKY+DS+KDGF +IRE+ K F  LG
Sbjct: 74  NPKIQVQSKATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFLNIREVTKAFSFLG 133

Query: 62  SVIPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           S+ PFYKA YAM +ADA+ DGLISEAEL+KLIDY  KIIKKK
Sbjct: 134 SINPFYKAQYAMTFADANNDGLISEAELEKLIDYAYKIIKKK 175

[Close]

BLAST of Bhi04G000303 vs. ExPASy TrEMBL
Match: A0A0A0LRC8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.5e-31
Identity = 75/100 (75.00%), Postives = 83/100 (83.00%), Query Frame = 0


Query: 4   KKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSV 63
           KKQL DK Q SS      KQSN+PLSRE+IKEIFKYHDSNKDGF SIREL K F S+GS+
Sbjct: 78  KKQLLDKAQGSS------KQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMGSI 137

Query: 64  IPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           +PF KA YAMAYAD D+DGL+SEAELDKLIDY +KIIKKK
Sbjct: 138 VPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]

BLAST of Bhi04G000303 vs. ExPASy TrEMBL
Match: A0A5A7V4R9 (Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00180 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.1e-29
Identity = 73/100 (73.00%), Postives = 81/100 (81.00%), Query Frame = 0


Query: 4   KKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSV 63
           KKQL DK Q+S       +QSN+PLSRE+IKEIFKYHDSNKDGF SIREL K F SLGS+
Sbjct: 74  KKQLLDKAQDS------LRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGSI 133

Query: 64  IPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           +PF KA +AMAYAD D+DGLISEAEL KLIDY  KIIKKK
Sbjct: 134 VPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

[Close]

BLAST of Bhi04G000303 vs. ExPASy TrEMBL
Match: A0A1S3BDW2 (probable calcium-binding protein CML11 OS=Cucumis melo OX=3656 GN=LOC103488544 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.1e-29
Identity = 73/100 (73.00%), Postives = 81/100 (81.00%), Query Frame = 0


Query: 4   KKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLGSV 63
           KKQL DK Q+S       +QSN+PLSRE+IKEIFKYHDSNKDGF SIREL K F SLGS+
Sbjct: 74  KKQLLDKAQDS------LRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGSI 133

Query: 64  IPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           +PF KA +AMAYAD D+DGLISEAEL KLIDY  KIIKKK
Sbjct: 134 VPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

[Close]

BLAST of Bhi04G000303 vs. ExPASy TrEMBL
Match: A0A6J1GJT8 (probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC111454533 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.2e-28
Identity = 69/102 (67.65%), Postives = 83/102 (81.37%), Query Frame = 0


Query: 2   NQKKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLG 61
           NQK Q++ K+  SSTKVL  K SN+PLSRE+IK+IFKY+DS+KDGF +IRE+ K F  LG
Sbjct: 72  NQKIQVQGKVTKSSTKVLSEKISNVPLSREQIKKIFKYYDSDKDGFLNIREVTKAFSFLG 131

Query: 62  SVIPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           S+ PFYKA YAM +ADA+ DGLISE EL+KLIDY  KIIKKK
Sbjct: 132 SINPFYKAQYAMTFADANNDGLISEVELEKLIDYACKIIKKK 173

[Close]

BLAST of Bhi04G000303 vs. ExPASy TrEMBL
Match: A0A6J1GII5 (probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC111454483 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.7e-28
Identity = 69/102 (67.65%), Postives = 82/102 (80.39%), Query Frame = 0


Query: 2   NQKKQLRDKIQNSSTKVLDRKQSNIPLSRERIKEIFKYHDSNKDGFFSIRELAKTFDSLG 61
           N K Q++ K   SSTKVL  K SN+PLSRE+IK+IFKY+DS+KDGF +IRE+ K F  LG
Sbjct: 74  NPKIQVQSKATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFLNIREVTKAFSFLG 133

Query: 62  SVIPFYKAHYAMAYADADKDGLISEAELDKLIDYTNKIIKKK 104
           S+ PFYKA YAM +ADA+ DGLISEAEL+KLIDY  KIIKKK
Sbjct: 134 SINPFYKAQYAMTFADANNDGLISEAELEKLIDYAYKIIKKK 175

The following BLAST results are available for this feature:

BLAST of Bhi04G000303 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76650.1	8.2e-06	29.59	calmodulin-like 38	[more]
AT1G76650.3	8.2e-06	29.59	calmodulin-like 38	[more]
AT1G76640.1	3.5e-04	29.13	Calcium-binding EF-hand family protein	[more]

BLAST of Bhi04G000303 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRE6	1.2e-04	29.59	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]

BLAST of Bhi04G000303 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886759.1	7.9e-43	91.26	probable calcium-binding protein CML11 [Benincasa hispida]	[more]
XP_004152801.1	3.1e-31	75.00	probable calcium-binding protein CML11 [Cucumis sativus] >KGN62526.1 hypothetica...	[more]
XP_008445564.1	6.5e-29	73.00	PREDICTED: probable calcium-binding protein CML11 [Cucumis melo] >KAA0060885.1 p...	[more]
XP_022951799.1	2.5e-28	67.65	probable calcium-binding protein CML11 [Cucurbita moschata]	[more]
XP_022951742.1	5.5e-28	67.65	probable calcium-binding protein CML11 [Cucurbita moschata]	[more]

BLAST of Bhi04G000303 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC8	1.5e-31	75.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359950 PE=4 SV=1	[more]
A0A5A7V4R9	3.1e-29	73.00	Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BDW2	3.1e-29	73.00	probable calcium-binding protein CML11 OS=Cucumis melo OX=3656 GN=LOC103488544 P...	[more]
A0A6J1GJT8	1.2e-28	67.65	probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1GII5	2.7e-28	67.65	probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 31..91 e-value: 1.1E-8 score: 35.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 28..63 score: 11.38912
None	No IPR available	GENE3D	1.10.238.10		coord: 1..98 e-value: 3.0E-12 score: 48.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 77..89
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 24..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M000303	Bhi04M000303	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding