Bhi04G000296 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G000296
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	DPH4 homolog
Location	chr4: 7570986 .. 7573609 (-)
RNA-Seq Expression	Bhi04G000296
Synteny	Bhi04G000296

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCTTTATGCCTCTCGATTTTCACGCCTGTTCCCGTTGCGTCGCACCATCCCGCCGCCGCCATCTCCGTCGTGCTCAGTGCCGCATCCTTTCTTCGCCGATCTTCTCCCCTTCGTTGGTCTCAGGTATTTTCTACATTACATATATATCTTTTGAGTTTCATTCCTTACAATGGATGAACGTCTGTAAGCTCAAACGAACGGGAAGATCGGTTCGATTTGTGAATTAACTTTTTCTAATCAAGGACTTTGGACATTCTTTTGGTTGTTCATTCTTACAATAGAGATTATTGGTTTAGATATCGGTTTAGCAAAAAAACATAGAACCGAAGCTTGTGCTAAATGATGTTTTTTATGTAATGGAAGAGCTTCAGTTGAATATCCTGTAGCTGTATGTTTTATTGCGCTACGCTGCGGTTCTGTGGTTTGTAGAGATTGAAATTGTGTGTTTTGAAATCGTTGACAGCCCTGTAATCTTATGAGTTCAGTAACTCAAGAAAAGTAAGCTATCTATAAGGACAAACTAGAAGGAAACTTCCCCACAATACCTTAATATCTCATACTTTTGGGATTCTGGTAACTATTTCATTTGTTCTAGGAATTTGATATCATCTTTTCCATTAAGCGTGCAATTAGCACTTTCAGTATCTTTACAATTTACCCCTTTTTAACAGGCACCACTGTAATTAGCTTATAGTTTCCATTGCATATATCTCAAGGTTGTTGGAAATTGATTCTATCCCACAATGAGTTCAAGCAGTGGCTCCATTGGTGAAACTTATTATGATGTACTGTCTTTGAAAGAAGATGCTAGCTACGACGAAATTCGAGCCAGCTATCGGTCTGCTCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATCCAGACGACATTGCGTTGGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTTGGCAGCTCAATGTCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGTTTAGAGGATATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTTTTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGAAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTACCTGCTTCCGTTGTTTTACCTTGTGGTTCTTGCTCTTTGAAGGTTCGTTTGCTAATTGATTCGAATTCAAGTGTTTCGATTGATTGATGAATTTGAATGATTAAAATCACACTGACTTCTTTAAGAACCTTGGCATTTCTTTCTTGAATGATTTCGATGTTTTAGAGAGCTCGAGTGTGTTGTGCTCTTTCTTGGGTAGAGTTTCCTTGGGTTAAGATAGAACAGTTCCCAGGGGATGTCCAAATGAAGAGTTGGACAAACTCTGTGAGGTGGAAGAAGCATTTGTGTTTGTTGGGGTAGGATTGACCACAAACATTCGTCAAAACAGAGAAAAAAACAGGTTTGATAGGGTAAAAGTTCTTAGTTATTTTAGTTATATGTTTGTACTTGAGGATTGAGGCTGAGATGTTGACACATGTAGGGCATAGAAGTGCACACATGGAGACTTAAGAAGCTGCTACCCCAACCACTTCAGTGAGCTGTTCTAACTTCTTTAATGCAATTATGCAACTTTGTTGAGTGAAGTTGAACAACTTTCCTAGGCTATTTGGTTCAATTTTGGACTTGTGGATTGTGGGGTATTATAGGCATCTGAAAGACATCAGCTTGAAGATTTTGTCTATCGACTGAATTAATCAAGATTTGAGAGAAGTATAACCAGAGTGTGTTTTGATGTAACAATTCTACCTGCTTTTGATACCATTGAGATGGGGAAAAATGCATTACAGCTTCCTTCTCTGAACTGACTGCTAGCAATTTGTGGTGCTGTGTAATATAAAGATGGGAAGAGAGATTTCCCCCCTTCTCCCTCGGAGCCTTGGTTAGTTCATTCTCAAGGTAGCATATCAATTAAATGATTTGATTTGTTCGCGTTAGGGGTGGTTTGGATTGTAGATATTATTTCATGGGTATAGATATTAAATGTCTGAAATTTTTTGTTTTCTTTTCTTTTATGAGTGACGAAGAGTTTTAAATGTATGAGATAATTAATGTCCGGGTTTGGATGTGTTTGATAATTAATATTTGGAAGTTAAAAGAA

mRNA sequence

GTCTTTATGCCTCTCGATTTTCACGCCTGTTCCCGTTGCGTCGCACCATCCCGCCGCCGCCATCTCCGTCGTGCTCAGTGCCGCATCCTTTCTTCGCCGATCTTCTCCCCTTCGTTGGTCTCAGGTTGTTGGAAATTGATTCTATCCCACAATGAGTTCAAGCAGTGGCTCCATTGGTGAAACTTATTATGATGTACTGTCTTTGAAAGAAGATGCTAGCTACGACGAAATTCGAGCCAGCTATCGGTCTGCTCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATCCAGACGACATTGCGTTGGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTTGGCAGCTCAATGTCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGTTTAGAGGATATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTTTTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGAAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTACCTGCTTCCGTTGTTTTACCTTGTGGTTCTTGCTCTTTGAAGGTTCGTTTGCTAATTGATTCGAATTCAAGTGTTTCGATTGATTGATGAATTTGAATGATTAAAATCACACTGACTTCTTTAAGAACCTTGGCATTTCTTTCTTGAATGATTTCGATGTTTTAGAGAGCTCGAGTGTGTTGTGCTCTTTCTTGGGTAGAGTTTCCTTGGGTTAAGATAGAACAGTTCCCAGGGGATGTCCAAATGAAGAGTTGGACAAACTCTGTGAGGTGGAAGAAGCATTTGTGTTTGTTGGGGTAGGATTGACCACAAACATTCGTCAAAACAGAGAAAAAAACAGGTTTGATAGGGTAAAAGTTCTTAGTTATTTTAGTTATATGTTTGTACTTGAGGATTGAGGCTGAGATGTTGACACATGTAGGGCATAGAAGTGCACACATGGAGACTTAAGAAGCTGCTACCCCAACCACTTCAGTGAGCTGTTCTAACTTCTTTAATGCAATTATGCAACTTTGTTGAGTGAAGTTGAACAACTTTCCTAGGCTATTTGGTTCAATTTTGGACTTGTGGATTGTGGGGTATTATAGGCATCTGAAAGACATCAGCTTGAAGATTTTGTCTATCGACTGAATTAATCAAGATTTGAGAGAAGTATAACCAGAGTGTGTTTTGATGTAACAATTCTACCTGCTTTTGATACCATTGAGATGGGGAAAAATGCATTACAGCTTCCTTCTCTGAACTGACTGCTAGCAATTTGTGGTGCTGTGTAATATAAAGATGGGAAGAGAGATTTCCCCCCTTCTCCCTCGGAGCCTTGGTTAGTTCATTCTCAAGGTAGCATATCAATTAAATGATTTGATTTGTTCGCGTTAGGGGTGGTTTGGATTGTAGATATTATTTCATGGGTATAGATATTAAATGTCTGAAATTTTTTGTTTTCTTTTCTTTTATGAGTGACGAAGAGTTTTAAATGTATGAGATAATTAATGTCCGGGTTTGGATGTGTTTGATAATTAATATTTGGAAGTTAAAAGAA

Coding sequence (CDS)

ATGAGTTCAAGCAGTGGCTCCATTGGTGAAACTTATTATGATGTACTGTCTTTGAAAGAAGATGCTAGCTACGACGAAATTCGAGCCAGCTATCGGTCTGCTCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATCCAGACGACATTGCGTTGGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTTGGCAGCTCAATGTCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGTTTAGAGGATATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTTTTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGAAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTACCTGCTTCCGTTGTTTTACCTTGTGGTTCTTGCTCTTTGAAGGTTCGTTTGCTAATTGATTCGAATTCAAGTGTTTCGATTGATTGA

Protein sequence

MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGDYFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID

Homology

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BLAST of Bhi04G000296 vs. TAIR 10
Match: AT4G10130.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 191.4 bits (485), Expect = 6.1e-49
Identity = 94/171 (54.97%), Postives = 124/171 (72.51%), Query Frame = 0


Query: 8   IGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQKAW 67
           + ETYY++LS+KEDASY+EIR SYRSA+L+ HPDKL      S  D    E++ K+QKAW
Sbjct: 8   VHETYYEILSVKEDASYEEIRNSYRSAILHSHPDKLNNTSRSSSDD----EKFLKIQKAW 67

Query: 68  EVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGDYFFIDSG 127
           EVL  +  R  YD +L++++ D I A+ IS+EDM VE  G+V++LFYQCRCGDYF +DS 
Sbjct: 68  EVLSDAELRVVYDNDLRSSRHDGITADEISIEDMSVEITGDVIDLFYQCRCGDYFCVDSS 127

Query: 128 ELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSI 179
           EL  MG+ LLR+G  V ++ L A  ASVVLPCGSCSLK R+ +DS+  + I
Sbjct: 128 ELGTMGFALLRDGDFVYVKRLGAFVASVVLPCGSCSLKTRVWVDSDMKIPI 174

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BLAST of Bhi04G000296 vs. TAIR 10
Match: AT1G74250.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.4e-07
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 0


Query: 1  MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
          M+SSS S    +Y+VL + +++S DEIR+SYR   L  HPDKL      S  +  A  ++
Sbjct: 1  MASSSRSEKRCHYEVLGISKESSPDEIRSSYRRLALQRHPDKLMKAAGLSEAE--ATAQF 60

Query: 61 FKVQKAWEVLGSSMSRASYD 81
           ++  A+EVL     RA YD
Sbjct: 61 QELVHAYEVLSDPKERAWYD 78

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BLAST of Bhi04G000296 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.9e-06
Identity = 28/100 (28.00%), Postives = 52/100 (52.00%), Query Frame = 0


Query: 9   GETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQKAWE 68
           G++YYDVL + + AS ++I+ +YR   L +HPDK Q        ++ A  ++ ++  A+E
Sbjct: 24  GKSYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQG-------NEEATRKFAEINNAYE 83

Query: 69  VLGSSMSRASYD-------RELQAAKGDAIGAESISLEDM 102
           VL     R  Y+       ++  A  G   G   ++++D+
Sbjct: 84  VLSDEEKREIYNKYGEEGLKQFSANGGRGGGGGGMNMQDI 116

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BLAST of Bhi04G000296 vs. TAIR 10
Match: AT4G37480.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.9e-06
Identity = 27/74 (36.49%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 10  ETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQKAWEV 69
           E  YD+L++ E +S  EI+AS+R      HPD ++     S  D     R+ ++  A+E+
Sbjct: 55  ENAYDILNVSETSSIAEIKASFRRLAKETHPDLIE-----SKKDPSNSRRFVQILAAYEI 114

Query: 70  LGSSMSRASYDREL 84
           L  S  RA YDR L
Sbjct: 115 LSDSEKRAHYDRYL 123

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BLAST of Bhi04G000296 vs. TAIR 10
Match: AT1G71000.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.1e-05
Identity = 25/75 (33.33%), Postives = 42/75 (56.00%), Query Frame = 0


Query: 6  GSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQK 65
          G I +TYY++L +  D+S ++IR +Y      +HPD+      +S     A  R+ ++Q+
Sbjct: 3  GGIRQTYYEILGVAVDSSAEQIRRAYHKLAKIWHPDRWTKDPFRSGE---AKRRFQQIQE 62

Query: 66 AWEVLGSSMSRASYD 81
          A+ VL     R+SYD
Sbjct: 63 AYSVLSDERKRSSYD 74

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BLAST of Bhi04G000296 vs. ExPASy Swiss-Prot
Match: Q54CI5 (DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.4e-11
Identity = 45/164 (27.44%), Postives = 79/164 (48.17%), Query Frame = 0


Query: 2   SSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQ---------------AM 61
           ++S+    + YY++L +  DA  +EI+ SYR   L +HPDKL                 +
Sbjct: 6   NNSNKCENKNYYEILKVSIDADIEEIKKSYRKLALLYHPDKLNKEENIEENINNFSNCLV 65

Query: 62  CHKSHPDDIALERYFKVQKAWEVLGSSMSRASYDRELQAAKGDAIG-AESISLEDM-VVE 121
            + ++ ++   + +  +Q AWE L   + R  YD  L   K      ++ I L+DM  +E
Sbjct: 66  NNNNNNNNSNTKDFNDIQIAWETLKDDLLRKQYDSLLLEQKRQKYSVSDEIDLDDMEFIE 125

Query: 122 DKGEVVELFYQCRCGDYFFIDSGELDEMGYPLLRNGSKVSLRTL 149
           +  E V   Y CRCGD++ I   +L E    +  +G  +S++ +
Sbjct: 126 ENSEYV---YPCRCGDHYIITEDQLSEGSDVVCCSGCSLSIKVI 166

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BLAST of Bhi04G000296 vs. ExPASy Swiss-Prot
Match: P47138 (Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=JJJ3 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.6e-09
Identity = 50/173 (28.90%), Postives = 81/173 (46.82%), Query Frame = 0


Query: 11  TYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQKAWEVL 70
           T+Y++L +  DA+ DEI+ +YR+ LLN HPDKL    H    D ++     K+Q A+++L
Sbjct: 8   THYEILRIPSDATQDEIKKAYRNRLLNTHPDKLSKSIH----DTVSNVTINKIQDAYKIL 67

Query: 71  GSSMSRASYDR------ELQAAKGDAIGAESISLEDMVV-EDKGEVVELFYQCRCGDYFF 130
            +  +R  YDR      + Q       G +  SL+D    EDK E +    +C+    F 
Sbjct: 68  SNIKTRREYDRLILENYKRQGFHNCGDGLDEFSLDDFSFDEDKLEFMMNCPRCQFVGGFH 127

Query: 131 IDSGELDEMGYPLLRNGSKVSLRTLDALPAS-----VVLPCGSCSLKVRLLID 172
                LDE             +  +DA+  S     ++  C +CSL +++  D
Sbjct: 128 FSESLLDE------------CIDNVDAMERSHSGYQLLTQCSACSLWLKVNFD 164

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BLAST of Bhi04G000296 vs. ExPASy Swiss-Prot
Match: B9KH92 (Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 28/73 (38.36%), Postives = 44/73 (60.27%), Query Frame = 0


Query: 9  GETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQKAWE 68
          G  YY++L +  +AS +EI+ SYR  +  +HPD       K+  D  A E++ K+ +A+E
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPD-------KNPGDKKAEEKFKKISEAYE 62

Query: 69 VLGSSMSRASYDR 82
          VL +   RA+YDR
Sbjct: 63 VLSNPEKRAAYDR 68

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BLAST of Bhi04G000296 vs. ExPASy Swiss-Prot
Match: Q5P9E0 (Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 28/73 (38.36%), Postives = 44/73 (60.27%), Query Frame = 0


Query: 9  GETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFKVQKAWE 68
          G  YY++L +  +AS +EI+ SYR  +  +HPD       K+  D  A E++ K+ +A+E
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPD-------KNPGDKKAEEKFKKISEAYE 62

Query: 69 VLGSSMSRASYDR 82
          VL +   RA+YDR
Sbjct: 63 VLSNPEKRAAYDR 68

[Close]

BLAST of Bhi04G000296 vs. ExPASy Swiss-Prot
Match: Q253T6 (Chaperone protein DnaJ OS=Chlamydia felis (strain Fe/C-56) OX=264202 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.7e-07
Identity = 30/71 (42.25%), Postives = 42/71 (59.15%), Query Frame = 0


Query: 12 YYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFK-VQKAWEVL 71
          YYDVL + + AS +EI+ +YR   + +HPDK        +P D   E+ FK V +A+EVL
Sbjct: 3  YYDVLGVSKTASPEEIKKAYRKLAVKYHPDK--------NPGDAEAEKRFKEVSEAYEVL 62

Query: 72 GSSMSRASYDR 82
            +  R SYDR
Sbjct: 63 SDAQKRESYDR 65

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BLAST of Bhi04G000296 vs. NCBI nr
Match: XP_038884890.1 (DPH4 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 354.8 bits (909), Expect = 4.5e-94
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY
Sbjct: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
           YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 179

[Close]

BLAST of Bhi04G000296 vs. NCBI nr
Match: XP_038884887.1 (DPH4 homolog isoform X1 [Benincasa hispida] >XP_038884888.1 DPH4 homolog isoform X1 [Benincasa hispida] >XP_038884889.1 DPH4 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 330.9 bits (847), Expect = 7.0e-87
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY
Sbjct: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLK 166
           YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLK
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLK 165

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BLAST of Bhi04G000296 vs. NCBI nr
Match: XP_008444636.1 (PREDICTED: DPH4 homolog [Cucumis melo])

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-86
Identity = 167/179 (93.30%), Postives = 172/179 (96.09%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSG+IGETYYDVLSL+EDAS+DEIRASYRSALLNFHPDKLQAMC KSHPDDI  ERY
Sbjct: 1   MSSSSGAIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGS  SRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSPKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
            FFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKV L+ID+NSSVSID
Sbjct: 121 CFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVLLVIDTNSSVSID 179

[Close]

BLAST of Bhi04G000296 vs. NCBI nr
Match: XP_022996470.1 (DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima])

HSP 1 Score: 328.9 bits (842), Expect = 2.6e-86
Identity = 167/179 (93.30%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSGSIGETYYDVLSL+EDASYDEIRASYRSALLNFHPDKLQAMCHKSH DDIA ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
            KVQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVELFYQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
           YFFIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSLKVRLLIDSNSSV  D
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSNSSVLND 179

[Close]

BLAST of Bhi04G000296 vs. NCBI nr
Match: XP_022962179.1 (DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moschata] >KAG7029347.1 JJJ3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327.4 bits (838), Expect = 7.7e-86
Identity = 166/179 (92.74%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSGSIGETYYDVLSL+EDASYDEIRASYRSALLNFHPDKLQAMCHKSH DDIA ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
            KVQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVELFYQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
           YFFIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSL+VRLLIDSNSSV  D
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSNSSVLND 179

[Close]

BLAST of Bhi04G000296 vs. ExPASy TrEMBL
Match: A0A1S3BBM0 (DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 5.7e-87
Identity = 167/179 (93.30%), Postives = 172/179 (96.09%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSG+IGETYYDVLSL+EDAS+DEIRASYRSALLNFHPDKLQAMC KSHPDDI  ERY
Sbjct: 1   MSSSSGAIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGS  SRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSPKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
            FFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKV L+ID+NSSVSID
Sbjct: 121 CFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVLLVIDTNSSVSID 179

[Close]

BLAST of Bhi04G000296 vs. ExPASy TrEMBL
Match: A0A6J1K4U3 (DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.3e-86
Identity = 167/179 (93.30%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSGSIGETYYDVLSL+EDASYDEIRASYRSALLNFHPDKLQAMCHKSH DDIA ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
            KVQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVELFYQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
           YFFIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSLKVRLLIDSNSSV  D
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSNSSVLND 179

[Close]

BLAST of Bhi04G000296 vs. ExPASy TrEMBL
Match: A0A6J1HGB5 (DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.7e-86
Identity = 166/179 (92.74%), Postives = 169/179 (94.41%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSGSIGETYYDVLSL+EDASYDEIRASYRSALLNFHPDKLQAMCHKSH DDIA ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
            KVQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGEVVELFYQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
           YFFIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSL+VRLLIDSNSSV  D
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSNSSVLND 179

[Close]

BLAST of Bhi04G000296 vs. ExPASy TrEMBL
Match: A0A0A0LKX8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.7e-86
Identity = 164/173 (94.80%), Postives = 168/173 (97.11%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           M+S SGSIGETYYDVLSL+EDAS+DEIRASYRSALLNFHPDKLQAMCHKSHPDDI  ERY
Sbjct: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSS SRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSN 174
           YFFIDSGELDEMGYPLLRNGSKVSLRTL+ALPASVVLPCGSCSLKVRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 173

[Close]

BLAST of Bhi04G000296 vs. ExPASy TrEMBL
Match: A0A6J1KK19 (DPH4 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.2e-84
Identity = 160/179 (89.39%), Postives = 171/179 (95.53%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60
           MSSSSGSIGETYYDVLSL+EDAS+DEIRASYRSALLNFHPDKLQAMC KSHP+D+  ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 60

Query: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSSMSRA+YDRELQAAKG+A+GAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
           YFFIDSGEL+EMGYP+LR GSKVSLRTLDALPAS+VLPCGSCSLKVRLLIDS+S VSID
Sbjct: 121 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSID 179

The following BLAST results are available for this feature:

BLAST of Bhi04G000296 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10130.1	6.1e-49	54.97	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G74250.1	3.4e-07	40.00	DNAJ heat shock N-terminal domain-containing protein	[more]
AT3G62600.1	4.9e-06	28.00	DNAJ heat shock family protein	[more]
AT4G37480.1	4.9e-06	36.49	Chaperone DnaJ-domain superfamily protein	[more]
AT1G71000.1	1.1e-05	33.33	Chaperone DnaJ-domain superfamily protein	[more]

BLAST of Bhi04G000296 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54CI5	8.4e-11	27.44	DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1	[more]
P47138	1.6e-09	28.90	Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
B9KH92	2.5e-07	38.36	Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ...	[more]
Q5P9E0	2.5e-07	38.36	Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=d...	[more]
Q253T6	5.7e-07	42.25	Chaperone protein DnaJ OS=Chlamydia felis (strain Fe/C-56) OX=264202 GN=dnaJ PE=...	[more]

BLAST of Bhi04G000296 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884890.1	4.5e-94	100.00	DPH4 homolog isoform X2 [Benincasa hispida]	[more]
XP_038884887.1	7.0e-87	100.00	DPH4 homolog isoform X1 [Benincasa hispida] >XP_038884888.1 DPH4 homolog isoform...	[more]
XP_008444636.1	1.2e-86	93.30	PREDICTED: DPH4 homolog [Cucumis melo]	[more]
XP_022996470.1	2.6e-86	93.30	DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima]	[more]
XP_022962179.1	7.7e-86	92.74	DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moscha...	[more]

BLAST of Bhi04G000296 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BBM0	5.7e-87	93.30	DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1	[more]
A0A6J1K4U3	1.3e-86	93.30	DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1	[more]
A0A6J1HGB5	3.7e-86	92.74	DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1	[more]
A0A0A0LKX8	3.7e-86	94.80	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1	[more]
A0A6J1KK19	1.2e-84	89.39	DPH4 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 31..46 score: 34.71 coord: 55..75 score: 26.18 coord: 75..94 score: 24.62 coord: 13..31 score: 38.21
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 10..75 e-value: 3.0E-9 score: 46.7
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 11..80 e-value: 1.9E-14 score: 53.5
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 11..83 score: 14.9563
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 11..71 e-value: 9.02903E-14 score: 60.6369
IPR036671	DPH-type metal-binding domain superfamily	GENE3D	3.10.660.10	DPH Zinc finger	coord: 87..179 e-value: 1.6E-16 score: 61.5
IPR036671	DPH-type metal-binding domain superfamily	SUPERFAMILY	144217	CSL zinc finger	coord: 92..172
IPR007872	DPH-type metal-binding domain	PFAM	PF05207	zf-CSL	coord: 95..169 e-value: 1.1E-12 score: 47.4
IPR007872	DPH-type metal-binding domain	PROSITE	PS51074	DPH_MB	coord: 92..171 score: 14.955948
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 2..86 e-value: 4.0E-17 score: 64.1
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 10..83
None	No IPR available	PANTHER	PTHR21454:SF31	DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4	coord: 1..172
IPR044248	Diphthamide biosynthesis protein 3/4-like	PANTHER	PTHR21454	DPH3 HOMOLOG-RELATED	coord: 1..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M000296	Bhi04M000296	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding