Bhi03G002144 (gene) Wax gourd (B227) v1

Overview
NameBhi03G002144
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptiontRNA-uridine aminocarboxypropyltransferase
Locationchr3: 63716326 .. 63717927 (+)
RNA-Seq ExpressionBhi03G002144
SyntenyBhi03G002144
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACCCTAGACTTACCAGGACCCTAAGAAGACTTATATTTGGGCTTCGTTAGGAAAACTTGCATGATCATCGTACCCACACACCCTTCCATCGCATGATAAACCAATGCATCAATTATACTTTCTGAAATCTCGAAGGTGTTTTACTTTGAGAGACAAGTTTTGCAAGTCTGATTCTGGGTATAAACTTGTGACATTTGGACCTTCGAGGGTGGTACTTGTTGTGTCTAGACATGTTGAGCTATCTATTCGGCCACAAAACATCTAATTTTCCCCTTTAGTGACCCTTTGTGATTAGTTTCTCCTGACTGCAGTATACTTGACGAGGACGATAGGGGAACCTATGCTAGCAGTCGAATTTCGGGTCCGACTTCTTGTCTCAATGAGGTATTTTAGTTTTTTTTTTTTTTTTTTTTCAAAAATCAAAAGACATCCCTCGATTTTACTAGTTTTAAGTAGAGAGTAGATATCACAACAAGCTGATATCGGGTCTCACATCCGAAAGGGGTGTGATAGAGAAGCTAGGGCAAGAATCTAGAATATTGAGCACATTTTGAGGTTAGATGGGTACTTCCTCATGTAGCTAACCTCCAAAGACTATTTTTCATTCATTCTCGGGATGTAGCACGGGAGGCATTTGTACTAAAATCTCAAGAAACACGTGAAAGCAACGCATTGAAACGACATCGTTTCCTCGCGCATGGAGGCAACAAACAACCACAACCCAGCCGCCGGAAATTCCTCATCCTCGTCCTCGCCGCCACCACGGCGGTCAATTTGCCCTAATTGCAGCCGTCCACAACCAGTTTGCCTCTGCAAATTCCTTCCATCTTCACCAATCGCAACCAAAACAAAAATCATCCTCCTCCAACATCCTCACGAAGCACAGCACAAGCTCTCCACCACACCAATCCTCACAAAATGTCTCCTCAACGCAACCACAATCGTCAATCGCCGCCTCAAACCCGGCCTCTCCGAACTCCTCGACCGATCTCCTCCCGCCGTCTTCCTCTTCCCACCTACGCCTCACTCCACACCGCCGATCAACATCTCGGAACTCAACATAACCGCCAATCTGGTCCTGATTGCGTTCGACGGAACATGGAAGCACGCGAAGGAGATGGTGCGATCGAGCGAGGAGTTTCTGTCGAGATTTGCGAGAAGAGTTTGCTTGGATTTTGATGAGAGTGTGGAAGGCGGAAGCATATTTGATTCGGAGTTGATTCTGAGAAAGGAGCCGCACGGCGGTTGCGTTAGTACGATGGAGGCGGTGGCAAGATGTTTGAGAGTGATGGAACCAGATGGGGCGGCGATTGAGAAGAAATTGATTGAAGTTCTGAGGGAGATGGTTAGGTTACAGGCTGGGTTTTTGAAGCCCATGAATCCTAGGCCCAAATTGCTTAAGATTAAGAACAGGAAGAAGAAGATGAGTGAAGGGGAGGATGAAAATTAAAATTAATTTTTTAAATTTTGGCTTCTTTTGATTTTGTTTTTTTTTTCTTTCTTTTTTTCAATTTAGCTCTTAAAATATTTTAGTGTTGTTTTAATTTAATTTGAAATAATATCTTGCTTTTTGTTCAACTTAGGGAGATTTAGATACAGG

mRNA sequence

CGACCCTAGACTTACCAGGACCCTAAGAAGACTTATATTTGGGCTTCGTTAGGAAAACTTGCATGATCATCGTACCCACACACCCTTCCATCGCATGATAAACCAATGCATCAATTATACTTTCTGAAATCTCGAAGGTGTTTTACTTTGAGAGACAAGTTTTGCAAGTCTGATTCTGGGTATAAACTTGTGACATTTGGACCTTCGAGGGTGGTACTTGTTGTGTCTAGACATGTTGAGCTATCTATTCGGCCACAAAACATCTAATTTTCCCCTTTAGTGACCCTTTGTGATTAGTTTCTCCTGACTGCAGTATACTTGACGAGGACGATAGGGGAACCTATGCTAGCAGTCGAATTTCGGGTCCGACTTCTTGTCTCAATGAGGTATTTTAGTTTTTTTTTTTTTTTTTTTTCAAAAATCAAAAGACATCCCTCGATTTTACTAGTTTTAAGTAGAGAGTAGATATCACAACAAGCTGATATCGGGTCTCACATCCGAAAGGGGTGTGATAGAGAAGCTAGGGCAAGAATCTAGAATATTGAGCACATTTTGAGGTTAGATGGGTACTTCCTCATGTAGCTAACCTCCAAAGACTATTTTTCATTCATTCTCGGGATGTAGCACGGGAGGCATTTGTACTAAAATCTCAAGAAACACGTGAAAGCAACGCATTGAAACGACATCGTTTCCTCGCGCATGGAGGCAACAAACAACCACAACCCAGCCGCCGGAAATTCCTCATCCTCGTCCTCGCCGCCACCACGGCGGTCAATTTGCCCTAATTGCAGCCGTCCACAACCAGTTTGCCTCTGCAAATTCCTTCCATCTTCACCAATCGCAACCAAAACAAAAATCATCCTCCTCCAACATCCTCACGAAGCACAGCACAAGCTCTCCACCACACCAATCCTCACAAAATGTCTCCTCAACGCAACCACAATCGTCAATCGCCGCCTCAAACCCGGCCTCTCCGAACTCCTCGACCGATCTCCTCCCGCCGTCTTCCTCTTCCCACCTACGCCTCACTCCACACCGCCGATCAACATCTCGGAACTCAACATAACCGCCAATCTGGTCCTGATTGCGTTCGACGGAACATGGAAGCACGCGAAGGAGATGGTGCGATCGAGCGAGGAGTTTCTGTCGAGATTTGCGAGAAGAGTTTGCTTGGATTTTGATGAGAGTGTGGAAGGCGGAAGCATATTTGATTCGGAGTTGATTCTGAGAAAGGAGCCGCACGGCGGTTGCGTTAGTACGATGGAGGCGGTGGCAAGATGTTTGAGAGTGATGGAACCAGATGGGGCGGCGATTGAGAAGAAATTGATTGAAGTTCTGAGGGAGATGGTTAGGTTACAGGCTGGGTTTTTGAAGCCCATGAATCCTAGGCCCAAATTGCTTAAGATTAAGAACAGGAAGAAGAAGATGAGTGAAGGGGAGGATGAAAATTAAAATTAATTTTTTAAATTTTGGCTTCTTTTGATTTTGTTTTTTTTTTCTTTCTTTTTTTCAATTTAGCTCTTAAAATATTTTAGTGTTGTTTTAATTTAATTTGAAATAATATCTTGCTTTTTGTTCAACTTAGGGAGATTTAGATACAGG

Coding sequence (CDS)

ATGGAGGCAACAAACAACCACAACCCAGCCGCCGGAAATTCCTCATCCTCGTCCTCGCCGCCACCACGGCGGTCAATTTGCCCTAATTGCAGCCGTCCACAACCAGTTTGCCTCTGCAAATTCCTTCCATCTTCACCAATCGCAACCAAAACAAAAATCATCCTCCTCCAACATCCTCACGAAGCACAGCACAAGCTCTCCACCACACCAATCCTCACAAAATGTCTCCTCAACGCAACCACAATCGTCAATCGCCGCCTCAAACCCGGCCTCTCCGAACTCCTCGACCGATCTCCTCCCGCCGTCTTCCTCTTCCCACCTACGCCTCACTCCACACCGCCGATCAACATCTCGGAACTCAACATAACCGCCAATCTGGTCCTGATTGCGTTCGACGGAACATGGAAGCACGCGAAGGAGATGGTGCGATCGAGCGAGGAGTTTCTGTCGAGATTTGCGAGAAGAGTTTGCTTGGATTTTGATGAGAGTGTGGAAGGCGGAAGCATATTTGATTCGGAGTTGATTCTGAGAAAGGAGCCGCACGGCGGTTGCGTTAGTACGATGGAGGCGGTGGCAAGATGTTTGAGAGTGATGGAACCAGATGGGGCGGCGATTGAGAAGAAATTGATTGAAGTTCTGAGGGAGATGGTTAGGTTACAGGCTGGGTTTTTGAAGCCCATGAATCCTAGGCCCAAATTGCTTAAGATTAAGAACAGGAAGAAGAAGATGAGTGAAGGGGAGGATGAAAATTAA

Protein sequence

MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISELNITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEPHGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRKKKMSEGEDEN
Homology
BLAST of Bhi03G002144 vs. TAIR 10
Match: AT2G41750.1 (DTW domain-containing protein )

HSP 1 Score: 232.6 bits (592), Expect = 3.4e-61
Identity = 135/247 (54.66%), Postives = 168/247 (68.02%), Query Frame = 0

Query: 23  RRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPHEAQHKLSTTPILTKCLLNATTI 82
           RR IC NC RP  +CLC  LP+  I T T+II+L HPHE++HKL+TTP+LTK LLN T I
Sbjct: 10  RRQICDNCDRPNAICLCHVLPADLIPTNTEIIILHHPHESRHKLNTTPLLTKSLLNVTRI 69

Query: 83  VNRRL------KPGLSELLDRSPPAVFLFPPTPHSTPPINISE---LNITAN-------- 142
             RRL        G + L   +PP ++LFP +P S+P + ISE   LN+  +        
Sbjct: 70  PARRLLRRHISTAGGTAL--PAPPTIYLFPSSP-SSPAVTISEFKSLNLLNHREISNPPP 129

Query: 143 LVLIAFDGTWKHAKEMVRSSEEFLSRF-ARRVCLD--FDESVEGGSIFDSELILRKEPHG 202
           L LI FD TWKHAKEMV++SE  L    A RVCLD   D SV GG+I+DSEL+LRKEP G
Sbjct: 130 LRLIVFDATWKHAKEMVKASEVVLREAGAVRVCLDTEIDASVSGGTIYDSELVLRKEPFG 189

Query: 203 GCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRKKK 250
           GCV+T EAVARCL  +EPDG  IE+KLI VL+EMVR Q+ +LKPM PRPKLLK + + ++
Sbjct: 190 GCVTTAEAVARCLGAIEPDGEEIERKLISVLKEMVRFQSKYLKPMKPRPKLLKKRFQNQQ 249

BLAST of Bhi03G002144 vs. TAIR 10
Match: AT5G54880.1 (DTW domain-containing protein )

HSP 1 Score: 42.7 bits (99), Expect = 4.9e-04
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 0

Query: 23 RRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPHEAQHKLSTTPILTKCLLN 79
          +R  CP+C +P  +CLCK + S     +  I +LQH  E +H L++T I    L N
Sbjct: 44 KRPTCPSCDKPSQLCLCKKMRSPCFDNQVSITILQHSLERKHALNSTRIARLGLKN 99

BLAST of Bhi03G002144 vs. ExPASy Swiss-Prot
Match: Q8NBA8 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Homo sapiens OX=9606 GN=DTWD2 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 61/210 (29.05%), Postives = 96/210 (45.71%), Query Frame = 0

Query: 20  PPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPHEAQHKLSTTPILTKCLL-- 79
           P  RR  C  CSRPQ VCLC FLP+ P+   T + ++QHP E    L T P+L  CL   
Sbjct: 62  PAERRPECTRCSRPQKVCLCPFLPAHPLHISTHLYIIQHPAEENKVLRTVPLLAACLPQD 121

Query: 80  NATTIVNRRLK----PGLSELLDRSPPAVFLFPPTPHSTPPINISELNITANL---VLIA 139
                + RR      P LS +  R    + L+P         N+ E  + + +    +I 
Sbjct: 122 KCKVKIGRRFSEERDPELSTVC-RKSGTLILYP----GAEAANLEEFILDSPVYPSTIII 181

Query: 140 FDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEPHGGCVSTMEA 199
            DGTW  AK++          F +       + V+  +   S+ ++R +P   C+ST+E 
Sbjct: 182 IDGTWSQAKDI----------FYKNSLFRHPKQVQLKTSISSQYVIRMQPTNRCLSTLEC 241

Query: 200 VARCLRVMEPDGAAIEKKLIEVLREMVRLQ 221
            A  L ++E +   I++ L+  L+ +   Q
Sbjct: 242 AAVALSILEKNN-YIQETLLRPLQALCSFQ 255

BLAST of Bhi03G002144 vs. ExPASy Swiss-Prot
Match: Q4R7M4 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Macaca fascicularis OX=9541 GN=DTWD2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 61/210 (29.05%), Postives = 96/210 (45.71%), Query Frame = 0

Query: 20  PPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPHEAQHKLSTTPILTKCLL-- 79
           P  RR  C  CSRPQ VCLC FLP+ P+   T + ++QHP E    L T P+L  CL   
Sbjct: 62  PAKRRPECSRCSRPQKVCLCPFLPAHPLHISTHLYIIQHPAEENKVLRTVPLLAACLPQD 121

Query: 80  NATTIVNRRLK----PGLSELLDRSPPAVFLFPPTPHSTPPINISELNITANL---VLIA 139
                + RR      P LS +  R    + L+P         N+ E  + + +    +I 
Sbjct: 122 KCKVKIGRRFSEERDPELSTVC-RKSGTLILYP----GAEAANLEEFILDSPVYPSTIII 181

Query: 140 FDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEPHGGCVSTMEA 199
            DGTW  AK++          F +       + V+  +   S+ ++R +P   C+ST+E 
Sbjct: 182 IDGTWSQAKDI----------FYKNSLFRHPKQVQLKTSISSQYVIRMQPTNRCLSTLEC 241

Query: 200 VARCLRVMEPDGAAIEKKLIEVLREMVRLQ 221
            A  L ++E +   I++ L+  L+ +   Q
Sbjct: 242 AAVALSILEKNN-YIQETLLRPLQALCSFQ 255

BLAST of Bhi03G002144 vs. ExPASy Swiss-Prot
Match: Q9D0U1 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.1e-13
Identity = 65/241 (26.97%), Postives = 103/241 (42.74%), Query Frame = 0

Query: 23  RRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPHEAQHKLSTTPILTKCLL--NAT 82
           RR  C  CSRPQ VCLC +LP  P+   T + ++QHP E    L T P+L  CL     T
Sbjct: 65  RRPECGRCSRPQKVCLCPYLPVRPLQISTHLYIIQHPAEESRVLRTVPLLAACLPPDRCT 124

Query: 83  TIVNRRLKPGLSELLD---RSPPAVFLFPPTPHSTPPINISELNITANL---VLIAFDGT 142
             + RR        L    R    + L+P         N+ E  + + +    +I  DGT
Sbjct: 125 VKIGRRFSEERDVELATVCRDSGTLILYP----GAEATNLEEFILDSPVYPSTIILIDGT 184

Query: 143 WKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEPHGGCVSTMEAVARC 202
           W  AK++          F +       + V+  +   S+ ++R +P   C+ST+E  A  
Sbjct: 185 WSQAKDI----------FYKNSLFRLPKQVQLKTSVCSQYVIRMQPTNRCLSTLECAAVA 244

Query: 203 LRVMEPDGAAIEKKLIEVLREM----------VRLQAGFLKPMNPRPKLLKIKNRKKKMS 246
           L ++E +   I++ L+  L+ +          +RL   +L      PK +    RK +  
Sbjct: 245 LSILEKNN-CIQETLLRPLQALCSFQLQHGAQIRLSKEYLLRNGLYPKPMPKNKRKLRKM 290

BLAST of Bhi03G002144 vs. NCBI nr
Match: XP_038883027.1 (tRNA-uridine aminocarboxypropyltransferase 2 [Benincasa hispida])

HSP 1 Score: 494.6 bits (1272), Expect = 5.1e-136
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60
           MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH
Sbjct: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60

Query: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120
           EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL
Sbjct: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120

Query: 121 NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP 180
           NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP
Sbjct: 121 NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP 180

Query: 181 HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK 240
           HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK
Sbjct: 181 HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK 240

Query: 241 KKMSEGEDEN 251
           KKMSEGEDEN
Sbjct: 241 KKMSEGEDEN 250

BLAST of Bhi03G002144 vs. NCBI nr
Match: KAA0043562.1 (DTW domain-containing protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 431.8 bits (1109), Expect = 4.0e-117
Identity = 223/250 (89.20%), Postives = 235/250 (94.00%), Query Frame = 0

Query: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60
           ME+TNNHN AAG   SSSSPPPRR ICPNC+RPQPVCLCKFLPSSPIATKTKII+LQHPH
Sbjct: 1   MESTNNHNFAAG--YSSSSPPPRRPICPNCNRPQPVCLCKFLPSSPIATKTKIIILQHPH 60

Query: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120
           E+QHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLD+SPPAVFLFPPTPHS+PPINIS+L
Sbjct: 61  ESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSSPPINISDL 120

Query: 121 NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP 180
           NIT +LVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLD DESVEGGSIFDSELILRKEP
Sbjct: 121 NITEDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVEGGSIFDSELILRKEP 180

Query: 181 HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK 240
           HGGCVSTMEAVARCLRVME DGAA+E+KLI VLREMVRLQAGFLKPMNPRPKLL  KN K
Sbjct: 181 HGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLL--KNFK 240

Query: 241 KKMSEGEDEN 251
           +K S  EDEN
Sbjct: 241 QKTSVMEDEN 246

BLAST of Bhi03G002144 vs. NCBI nr
Match: TYK19777.1 (DTW domain-containing protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 428.7 bits (1101), Expect = 3.4e-116
Identity = 222/250 (88.80%), Postives = 234/250 (93.60%), Query Frame = 0

Query: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60
           ME+TNNHN AAG SSSS   PPRR ICPNC+RPQPVCLCKFLPSSPIATKTKII+LQHPH
Sbjct: 1   MESTNNHNFAAGYSSSSR--PPRRPICPNCNRPQPVCLCKFLPSSPIATKTKIIILQHPH 60

Query: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120
           E+QHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLD+SPPAVFLFPPTPHS+PPINIS+L
Sbjct: 61  ESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSSPPINISDL 120

Query: 121 NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP 180
           NIT +LVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLD DESVEGGSIFDSELILRKEP
Sbjct: 121 NITEDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVEGGSIFDSELILRKEP 180

Query: 181 HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK 240
           HGGCVSTMEAVARCLRVME DGAA+E+KLI VLREMVRLQAGFLKPMNPRPKLL  KN K
Sbjct: 181 HGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLL--KNFK 240

Query: 241 KKMSEGEDEN 251
           +K S  EDEN
Sbjct: 241 QKTSVMEDEN 246

BLAST of Bhi03G002144 vs. NCBI nr
Match: XP_023544253.1 (DTW domain-containing protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.4 bits (1082), Expect = 5.5e-114
Identity = 217/256 (84.77%), Postives = 233/256 (91.02%), Query Frame = 0

Query: 1   MEATN-NHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHP 60
           ME TN +HN AAG SS++SSPP RRSICPNC+RPQPVCLCKFLPS PIATKT+II+LQHP
Sbjct: 1   MEPTNHHHNSAAGYSSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHP 60

Query: 61  HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISE 120
           HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLDRSP AV+LFPPTPHS+PPINISE
Sbjct: 61  HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINISE 120

Query: 121 LNIT------ANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSE 180
           L++T      +NLVLIAFDGTWKHAKEMVRSSEEFLSRFARR+CLDFDESV+GGSIFDSE
Sbjct: 121 LDLTSSSSPDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGSIFDSE 180

Query: 181 LILRKEPHGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKL 240
           LILRKEPHGGCVSTMEAVARCLRVME DGA IE +LI VLREMVRLQAGFLKPMNPRPKL
Sbjct: 181 LILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAGFLKPMNPRPKL 240

Query: 241 LKIKNRKKKMSEGEDE 250
           LK K + K  SE EDE
Sbjct: 241 LKTKKKMKNKSEREDE 256

BLAST of Bhi03G002144 vs. NCBI nr
Match: XP_022967888.1 (DTW domain-containing protein 2 [Cucurbita maxima])

HSP 1 Score: 417.2 bits (1071), Expect = 1.0e-112
Identity = 216/256 (84.38%), Postives = 234/256 (91.41%), Query Frame = 0

Query: 1   MEATN-NHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHP 60
           ME TN +HN AAG SS+SSSPPPRRSICPNC+RPQ VCLCKFLPS PIATKT+II+LQHP
Sbjct: 1   MEPTNHHHNSAAGYSSASSSPPPRRSICPNCTRPQLVCLCKFLPSPPIATKTQIIILQHP 60

Query: 61  HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISE 120
           HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLDRSP AV+LFPPTPHS+PPINISE
Sbjct: 61  HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINISE 120

Query: 121 LNIT------ANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSE 180
           L++T      +NLVLIAFDGTWKHAKEMVRSSEEFLSRFARR+CLDFDESV+GG+IFDSE
Sbjct: 121 LDLTSSSSSDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGTIFDSE 180

Query: 181 LILRKEPHGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKL 240
           LILRKEPHGGCVSTMEAVARCLRVME DGA IE +LI VLREMVRLQAGFLKPMNPRPKL
Sbjct: 181 LILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAGFLKPMNPRPKL 240

Query: 241 LKIKNRKKKMSEGEDE 250
           +K+KN+    SE EDE
Sbjct: 241 VKMKNK----SEREDE 252

BLAST of Bhi03G002144 vs. ExPASy TrEMBL
Match: A0A5A7TJW5 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold335G00690 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.0e-117
Identity = 223/250 (89.20%), Postives = 235/250 (94.00%), Query Frame = 0

Query: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60
           ME+TNNHN AAG   SSSSPPPRR ICPNC+RPQPVCLCKFLPSSPIATKTKII+LQHPH
Sbjct: 1   MESTNNHNFAAG--YSSSSPPPRRPICPNCNRPQPVCLCKFLPSSPIATKTKIIILQHPH 60

Query: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120
           E+QHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLD+SPPAVFLFPPTPHS+PPINIS+L
Sbjct: 61  ESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSSPPINISDL 120

Query: 121 NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP 180
           NIT +LVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLD DESVEGGSIFDSELILRKEP
Sbjct: 121 NITEDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVEGGSIFDSELILRKEP 180

Query: 181 HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK 240
           HGGCVSTMEAVARCLRVME DGAA+E+KLI VLREMVRLQAGFLKPMNPRPKLL  KN K
Sbjct: 181 HGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLL--KNFK 240

Query: 241 KKMSEGEDEN 251
           +K S  EDEN
Sbjct: 241 QKTSVMEDEN 246

BLAST of Bhi03G002144 vs. ExPASy TrEMBL
Match: A0A5D3D883 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold307G00010 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.7e-116
Identity = 222/250 (88.80%), Postives = 234/250 (93.60%), Query Frame = 0

Query: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60
           ME+TNNHN AAG SSSS   PPRR ICPNC+RPQPVCLCKFLPSSPIATKTKII+LQHPH
Sbjct: 1   MESTNNHNFAAGYSSSSR--PPRRPICPNCNRPQPVCLCKFLPSSPIATKTKIIILQHPH 60

Query: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120
           E+QHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLD+SPPAVFLFPPTPHS+PPINIS+L
Sbjct: 61  ESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSSPPINISDL 120

Query: 121 NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP 180
           NIT +LVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLD DESVEGGSIFDSELILRKEP
Sbjct: 121 NITEDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVEGGSIFDSELILRKEP 180

Query: 181 HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK 240
           HGGCVSTMEAVARCLRVME DGAA+E+KLI VLREMVRLQAGFLKPMNPRPKLL  KN K
Sbjct: 181 HGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLL--KNFK 240

Query: 241 KKMSEGEDEN 251
           +K S  EDEN
Sbjct: 241 QKTSVMEDEN 246

BLAST of Bhi03G002144 vs. ExPASy TrEMBL
Match: A0A6J1HWG2 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467264 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 5.0e-113
Identity = 216/256 (84.38%), Postives = 234/256 (91.41%), Query Frame = 0

Query: 1   MEATN-NHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHP 60
           ME TN +HN AAG SS+SSSPPPRRSICPNC+RPQ VCLCKFLPS PIATKT+II+LQHP
Sbjct: 1   MEPTNHHHNSAAGYSSASSSPPPRRSICPNCTRPQLVCLCKFLPSPPIATKTQIIILQHP 60

Query: 61  HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISE 120
           HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLDRSP AV+LFPPTPHS+PPINISE
Sbjct: 61  HEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINISE 120

Query: 121 LNIT------ANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSE 180
           L++T      +NLVLIAFDGTWKHAKEMVRSSEEFLSRFARR+CLDFDESV+GG+IFDSE
Sbjct: 121 LDLTSSSSSDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGTIFDSE 180

Query: 181 LILRKEPHGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKL 240
           LILRKEPHGGCVSTMEAVARCLRVME DGA IE +LI VLREMVRLQAGFLKPMNPRPKL
Sbjct: 181 LILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAGFLKPMNPRPKL 240

Query: 241 LKIKNRKKKMSEGEDE 250
           +K+KN+    SE EDE
Sbjct: 241 VKMKNK----SEREDE 252

BLAST of Bhi03G002144 vs. ExPASy TrEMBL
Match: A0A0A0KW36 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G165850 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.5e-113
Identity = 216/250 (86.40%), Postives = 232/250 (92.80%), Query Frame = 0

Query: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60
           ME+ NNH+ AAG SSSS+  PPRRSIC NC+RPQPVCLCKFLPS PIATKTKII+LQHPH
Sbjct: 1   MESRNNHDLAAGYSSSST--PPRRSICSNCNRPQPVCLCKFLPSPPIATKTKIIILQHPH 60

Query: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120
           E+QHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLD+SP AVFLFPPTPHS+PPINIS+L
Sbjct: 61  ESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPHAVFLFPPTPHSSPPINISDL 120

Query: 121 NITANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSELILRKEP 180
           NIT +LVLIAFDGTWKHAKEMVRSSEE+LSRFARRVCLD DESVEGGSIFDSELILRKEP
Sbjct: 121 NITEDLVLIAFDGTWKHAKEMVRSSEEYLSRFARRVCLDIDESVEGGSIFDSELILRKEP 180

Query: 181 HGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLLKIKNRK 240
           HGGCVSTMEAVARCLRVME DGAA+E+KLI VLREMVRLQAGFLKPMNPRPKLL  K+ K
Sbjct: 181 HGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLL--KSLK 240

Query: 241 KKMSEGEDEN 251
           K+ S  EDEN
Sbjct: 241 KQTSVTEDEN 246

BLAST of Bhi03G002144 vs. ExPASy TrEMBL
Match: A0A6J1EL33 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435542 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.5e-112
Identity = 212/255 (83.14%), Postives = 230/255 (90.20%), Query Frame = 0

Query: 1   MEATNNHNPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQHPH 60
           ME TN+H     ++S++SSPP RRSICPNC+RPQPVCLCKFLPS PIATKT+II+LQHPH
Sbjct: 1   MEPTNHH-----HNSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPH 60

Query: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINISEL 120
           EAQHKLSTTPILTKCLLNATTIVNRRLKPGLS+LLDRSP AV+LFPPTPHS+PPINISEL
Sbjct: 61  EAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINISEL 120

Query: 121 NIT------ANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSEL 180
           ++T      +NLVLIAFDGTWKHAKEMVRSSEEFLSRFARR+CLDFDESV+GGSIFDSEL
Sbjct: 121 DLTSSSSPDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGSIFDSEL 180

Query: 181 ILRKEPHGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLL 240
           ILRKEPHGGCVSTMEAVARCLRVME DGA IE +LI VLREMVRLQAGFLKPMNPRPKLL
Sbjct: 181 ILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAGFLKPMNPRPKLL 240

Query: 241 KIKNRKKKMSEGEDE 250
           K K + K  SE EDE
Sbjct: 241 KTKKKTKNKSEREDE 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G41750.13.4e-6154.66DTW domain-containing protein [more]
AT5G54880.14.9e-0437.50DTW domain-containing protein [more]
Match NameE-valueIdentityDescription
Q8NBA81.6e-1529.05tRNA-uridine aminocarboxypropyltransferase 2 OS=Homo sapiens OX=9606 GN=DTWD2 PE... [more]
Q4R7M41.6e-1529.05tRNA-uridine aminocarboxypropyltransferase 2 OS=Macaca fascicularis OX=9541 GN=D... [more]
Q9D0U11.1e-1326.97tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 P... [more]
Match NameE-valueIdentityDescription
XP_038883027.15.1e-136100.00tRNA-uridine aminocarboxypropyltransferase 2 [Benincasa hispida][more]
KAA0043562.14.0e-11789.20DTW domain-containing protein 2 [Cucumis melo var. makuwa][more]
TYK19777.13.4e-11688.80DTW domain-containing protein 2 [Cucumis melo var. makuwa][more]
XP_023544253.15.5e-11484.77DTW domain-containing protein 2 [Cucurbita pepo subsp. pepo][more]
XP_022967888.11.0e-11284.38DTW domain-containing protein 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5A7TJW52.0e-11789.20tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5D3D8831.7e-11688.80tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1HWG25.0e-11384.38tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0KW366.5e-11386.40tRNA-uridine aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G1... [more]
A0A6J1EL335.5e-11283.14tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005636DTWSMARTSM01144DTW_2acoord: 23..225
e-value: 2.1E-43
score: 160.2
IPR005636DTWPFAMPF03942DTWcoord: 25..220
e-value: 3.6E-40
score: 137.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR21392:SF0DTW DOMAIN-CONTAINING PROTEIN 2coord: 14..235
IPR039262DTW domain-containing protein DTWD2/YfiPPANTHERPTHR21392UNCHARACTERIZEDcoord: 14..235

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M002144Bhi03M002144mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
molecular_function GO:0016432 tRNA-uridine aminocarboxypropyltransferase activity