Bhi03G002088 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G002088
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	ATP synthase epsilon chain, chloroplastic
Location	chr3: 61487986 .. 61488246 (-)
RNA-Seq Expression	Bhi03G002088
Synteny	Bhi03G002088

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGTAAATATTCTCGCATTTATTGCTACTGCACTGTTCATTCTACTTCTTACCACCGAACAAGCCTTTTATTTGGTAGGTAACATTGATGAAGCTACTGCGAAGGCTACGAACTTAGAAATGGAGAGCAAATTGATTGAAGAAATGACCTTAAATCTTAGTGTCCTGACTCCGAATCGAATTATTTGGGATTCAGAAGTGAAAGAAATCATTTTAGTTACGAATAGTGGACAAATTGGGTTCAATGCAATCATCTGA

mRNA sequence

ATGGAAGTAAATATTCTCGCATTTATTGCTACTGCACTGTTCATTCTACTTCTTACCACCGAACAAGCCTTTTATTTGGTAGGTAACATTGATGAAGCTACTGCGAAGGCTACGAACTTAGAAATGGAGAGCAAATTGATTGAAGAAATGACCTTAAATCTTAGTGTCCTGACTCCGAATCGAATTATTTGGGATTCAGAAGTGAAAGAAATCATTTTAGTTACGAATAGTGGACAAATTGGGTTCAATGCAATCATCTGA

Coding sequence (CDS)

ATGGAAGTAAATATTCTCGCATTTATTGCTACTGCACTGTTCATTCTACTTCTTACCACCGAACAAGCCTTTTATTTGGTAGGTAACATTGATGAAGCTACTGCGAAGGCTACGAACTTAGAAATGGAGAGCAAATTGATTGAAGAAATGACCTTAAATCTTAGTGTCCTGACTCCGAATCGAATTATTTGGGATTCAGAAGTGAAAGAAATCATTTTAGTTACGAATAGTGGACAAATTGGGTTCAATGCAATCATCTGA

Protein sequence

MEVNILAFIATALFILLLTTEQAFYLVGNIDEATAKATNLEMESKLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQIGFNAII

Homology

[Close]

BLAST of Bhi03G002088 vs. TAIR 10
Match: ATCG00470.1 (ATP synthase epsilon chain )

HSP 1 Score: 60.8 bits (146), Expect = 6.0e-10
Identity = 29/32 (90.62%), Postives = 30/32 (93.75%), Query Frame = 0


Query: 50 MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          MTLNL VLTPNRI+WDSEVKEIIL TNSGQIG
Sbjct: 1  MTLNLCVLTPNRIVWDSEVKEIILSTNSGQIG 32

[Close]

BLAST of Bhi03G002088 vs. TAIR 10
Match: ATCG00480.1 (ATP synthase subunit beta )

HSP 1 Score: 53.5 bits (127), Expect = 9.6e-08
Identity = 26/26 (100.00%), Postives = 26/26 (100.00%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEMESKL 47
           EQAFYLVGNIDEATAKATNLEMESKL
Sbjct: 471 EQAFYLVGNIDEATAKATNLEMESKL 496

[Close]

BLAST of Bhi03G002088 vs. ExPASy Swiss-Prot
Match: Q4VZG9 (ATP synthase epsilon chain, chloroplastic OS=Cucumis sativus OX=3659 GN=atpE PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-10
Identity = 32/32 (100.00%), Postives = 32/32 (100.00%), Query Frame = 0


Query: 50 MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG
Sbjct: 1  MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 32

[Close]

BLAST of Bhi03G002088 vs. ExPASy Swiss-Prot
Match: Q70XZ7 (ATP synthase epsilon chain, chloroplastic OS=Amborella trichopoda OX=13333 GN=atpE PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 6.5e-09
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 0


Query: 50 MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          MTLNL VLTPNRIIWDSEVKEIIL TNSGQIG
Sbjct: 1  MTLNLCVLTPNRIIWDSEVKEIILSTNSGQIG 32

[Close]

BLAST of Bhi03G002088 vs. ExPASy Swiss-Prot
Match: Q7YJW6 (ATP synthase epsilon chain, chloroplastic OS=Calycanthus floridus var. glaucus OX=212734 GN=atpE PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 6.5e-09
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 0


Query: 50 MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          MTLNL VLTPNRIIWDSEVKEIIL TNSGQIG
Sbjct: 1  MTLNLCVLTPNRIIWDSEVKEIILSTNSGQIG 32

[Close]

BLAST of Bhi03G002088 vs. ExPASy Swiss-Prot
Match: Q06GZ1 (ATP synthase epsilon chain, chloroplastic OS=Drimys granadensis OX=224735 GN=atpE PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 6.5e-09
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 0


Query: 50 MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          MTLNL VLTPNRIIWDSEVKEIIL TNSGQIG
Sbjct: 1  MTLNLCVLTPNRIIWDSEVKEIILSTNSGQIG 32

[Close]

BLAST of Bhi03G002088 vs. ExPASy Swiss-Prot
Match: Q0G9L3 (ATP synthase epsilon chain, chloroplastic OS=Liriodendron tulipifera OX=3415 GN=atpE PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 6.5e-09
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 0


Query: 50 MTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          MTLNL VLTPNRIIWDSEVKEIIL TNSGQIG
Sbjct: 1  MTLNLCVLTPNRIIWDSEVKEIILSTNSGQIG 32

[Close]

BLAST of Bhi03G002088 vs. NCBI nr
Match: VVW89794.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 92.8 bits (229), Expect = 1.5e-15
Identity = 50/59 (84.75%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 23 AFYLVGNIDEATAKATNLEMESKLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          AFYLV NIDEATAKA NLE   + IEEMTLNL VLTPNRIIWDSEVKEIIL TNSGQIG
Sbjct: 1  AFYLVSNIDEATAKAMNLESREQ-IEEMTLNLCVLTPNRIIWDSEVKEIILSTNSGQIG 58

[Close]

BLAST of Bhi03G002088 vs. NCBI nr
Match: RDY14683.1 (ATP synthase subunit beta, chloroplastic, partial [Mucuna pruriens])

HSP 1 Score: 72.0 bits (175), Expect = 2.8e-09
Identity = 43/61 (70.49%), Postives = 44/61 (72.13%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEMESKLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQI 80
           EQAFYLVG       K  N E     IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQI
Sbjct: 471 EQAFYLVG------YKLRNGEQ----IEEMTLNLCVLTPNRIVWDSEVKEIILPTNSGQI 521

Query: 81  G 82
           G
Sbjct: 531 G 521

[Close]

BLAST of Bhi03G002088 vs. NCBI nr
Match: KAF1876833.1 (hypothetical protein Lal_00033121 [Lupinus albus])

HSP 1 Score: 70.9 bits (172), Expect = 6.2e-09
Identity = 41/62 (66.13%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEM-ESKLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQ 80
           EQAFYLVG            E+ + + IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQ
Sbjct: 471 EQAFYLVG-----------YELRKGEQIEEMTLNLCVLTPNRIVWDSEVKEIILCTNSGQ 521

Query: 81  IG 82
           IG
Sbjct: 531 IG 521

[Close]

BLAST of Bhi03G002088 vs. NCBI nr
Match: KAF4381351.1 (hypothetical protein F8388_026450, partial [Cannabis sativa])

HSP 1 Score: 70.5 bits (171), Expect = 8.1e-09
Identity = 41/62 (66.13%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEMES-KLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQ 80
           EQAFYLVG            E+ + + IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQ
Sbjct: 132 EQAFYLVG-----------YELRNGEQIEEMTLNLCVLTPNRIVWDSEVKEIILSTNSGQ 182

Query: 81  IG 82
           IG
Sbjct: 192 IG 182

[Close]

BLAST of Bhi03G002088 vs. NCBI nr
Match: KAF4394665.1 (hypothetical protein G4B88_011126, partial [Cannabis sativa])

HSP 1 Score: 70.5 bits (171), Expect = 8.1e-09
Identity = 41/62 (66.13%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEMES-KLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQ 80
           EQAFYLVG            E+ + + IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQ
Sbjct: 132 EQAFYLVG-----------YELRNGEQIEEMTLNLCVLTPNRIVWDSEVKEIILSTNSGQ 182

Query: 81  IG 82
           IG
Sbjct: 192 IG 182

[Close]

BLAST of Bhi03G002088 vs. ExPASy TrEMBL
Match: A0A5K1HX57 (ATP-synt_DE_N domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS30491 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.4e-16
Identity = 50/59 (84.75%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 23 AFYLVGNIDEATAKATNLEMESKLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQIG 82
          AFYLV NIDEATAKA NLE   + IEEMTLNL VLTPNRIIWDSEVKEIIL TNSGQIG
Sbjct: 1  AFYLVSNIDEATAKAMNLESREQ-IEEMTLNLCVLTPNRIIWDSEVKEIILSTNSGQIG 58

[Close]

BLAST of Bhi03G002088 vs. ExPASy TrEMBL
Match: A0A371II26 (NADH dehydrogenase subunit 3 (Fragment) OS=Mucuna pruriens OX=157652 GN=atpB PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.4e-09
Identity = 43/61 (70.49%), Postives = 44/61 (72.13%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEMESKLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQI 80
           EQAFYLVG       K  N E     IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQI
Sbjct: 471 EQAFYLVG------YKLRNGEQ----IEEMTLNLCVLTPNRIVWDSEVKEIILPTNSGQI 521

Query: 81  G 82
           G
Sbjct: 531 G 521

[Close]

BLAST of Bhi03G002088 vs. ExPASy TrEMBL
Match: A0A6A5MZ22 (H(+)-transporting two-sector ATPase OS=Lupinus albus OX=3870 GN=Lal_00033121 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.0e-09
Identity = 41/62 (66.13%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEM-ESKLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQ 80
           EQAFYLVG            E+ + + IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQ
Sbjct: 471 EQAFYLVG-----------YELRKGEQIEEMTLNLCVLTPNRIVWDSEVKEIILCTNSGQ 521

Query: 81  IG 82
           IG
Sbjct: 531 IG 521

[Close]

BLAST of Bhi03G002088 vs. ExPASy TrEMBL
Match: A0A7J6GEG5 (H(+)-transporting two-sector ATPase (Fragment) OS=Cannabis sativa OX=3483 GN=F8388_026450 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-09
Identity = 41/62 (66.13%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEMES-KLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQ 80
           EQAFYLVG            E+ + + IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQ
Sbjct: 132 EQAFYLVG-----------YELRNGEQIEEMTLNLCVLTPNRIVWDSEVKEIILSTNSGQ 182

Query: 81  IG 82
           IG
Sbjct: 192 IG 182

[Close]

BLAST of Bhi03G002088 vs. ExPASy TrEMBL
Match: A0A7J6HHZ8 (H(+)-transporting two-sector ATPase OS=Cannabis sativa OX=3483 GN=G4B88_011126 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-09
Identity = 41/62 (66.13%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 21  EQAFYLVGNIDEATAKATNLEMES-KLIEEMTLNLSVLTPNRIIWDSEVKEIILVTNSGQ 80
           EQAFYLVG            E+ + + IEEMTLNL VLTPNRI+WDSEVKEIIL TNSGQ
Sbjct: 132 EQAFYLVG-----------YELRNGEQIEEMTLNLCVLTPNRIVWDSEVKEIILSTNSGQ 182

Query: 81  IG 82
           IG
Sbjct: 192 IG 182

The following BLAST results are available for this feature:

BLAST of Bhi03G002088 vs. TAIR 10


Match Name	E-value	Identity	Description
ATCG00470.1	6.0e-10	90.63	ATP synthase epsilon chain	[more]
ATCG00480.1	9.6e-08	100.00	ATP synthase subunit beta	[more]

BLAST of Bhi03G002088 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4VZG9	3.4e-10	100.00	ATP synthase epsilon chain, chloroplastic OS=Cucumis sativus OX=3659 GN=atpE PE=...	[more]
Q70XZ7	6.5e-09	93.75	ATP synthase epsilon chain, chloroplastic OS=Amborella trichopoda OX=13333 GN=at...	[more]
Q7YJW6	6.5e-09	93.75	ATP synthase epsilon chain, chloroplastic OS=Calycanthus floridus var. glaucus O...	[more]
Q06GZ1	6.5e-09	93.75	ATP synthase epsilon chain, chloroplastic OS=Drimys granadensis OX=224735 GN=atp...	[more]
Q0G9L3	6.5e-09	93.75	ATP synthase epsilon chain, chloroplastic OS=Liriodendron tulipifera OX=3415 GN=...	[more]

BLAST of Bhi03G002088 vs. NCBI nr


Match Name	E-value	Identity	Description
VVW89794.1	1.5e-15	84.75	unnamed protein product, partial [Nymphaea colorata]	[more]
RDY14683.1	2.8e-09	70.49	ATP synthase subunit beta, chloroplastic, partial [Mucuna pruriens]	[more]
KAF1876833.1	6.2e-09	66.13	hypothetical protein Lal_00033121 [Lupinus albus]	[more]
KAF4381351.1	8.1e-09	66.13	hypothetical protein F8388_026450, partial [Cannabis sativa]	[more]
KAF4394665.1	8.1e-09	66.13	hypothetical protein G4B88_011126, partial [Cannabis sativa]	[more]

BLAST of Bhi03G002088 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5K1HX57	7.4e-16	84.75	ATP-synt_DE_N domain-containing protein (Fragment) OS=Nymphaea colorata OX=21022...	[more]
A0A371II26	1.4e-09	70.49	NADH dehydrogenase subunit 3 (Fragment) OS=Mucuna pruriens OX=157652 GN=atpB PE=...	[more]
A0A6A5MZ22	3.0e-09	66.13	H(+)-transporting two-sector ATPase OS=Lupinus albus OX=3870 GN=Lal_00033121 PE=...	[more]
A0A7J6GEG5	3.9e-09	66.13	H(+)-transporting two-sector ATPase (Fragment) OS=Cannabis sativa OX=3483 GN=F83...	[more]
A0A7J6HHZ8	3.9e-09	66.13	H(+)-transporting two-sector ATPase OS=Cannabis sativa OX=3483 GN=G4B88_011126 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 30..50
IPR036771	F0F1 ATP synthase delta/epsilon subunit, N-terminal	GENE3D	2.60.15.10		coord: 50..84 e-value: 4.7E-5 score: 25.3
IPR036771	F0F1 ATP synthase delta/epsilon subunit, N-terminal	SUPERFAMILY	51344	Epsilon subunit of F1F0-ATP synthase N-terminal domain	coord: 50..82
IPR020546	ATP synthase, F1 complex, delta/epsilon subunit, N-terminal	PFAM	PF02823	ATP-synt_DE_N	coord: 52..82 e-value: 1.4E-5 score: 24.9
IPR007826	Photosystem II PsbM	PFAM	PF05151	PsbM	coord: 1..16 e-value: 1.2E-6 score: 28.2
IPR037269	Photosystem II PsbM superfamily	SUPERFAMILY	161033	Photosystem II reaction center protein M, PsbM	coord: 1..20

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M002088	Bhi03M002088	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015986	ATP synthesis coupled proton transport
biological_process	GO:0019684	photosynthesis, light reaction
biological_process	GO:0015979	photosynthesis
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0031090	organelle membrane
cellular_component	GO:0009523	photosystem II
cellular_component	GO:0045261	proton-transporting ATP synthase complex, catalytic core F(1)
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0016651	oxidoreductase activity, acting on NAD(P)H
molecular_function	GO:0046961	proton-transporting ATPase activity, rotational mechanism
molecular_function	GO:0046933	proton-transporting ATP synthase activity, rotational mechanism