Bhi03G001600 (gene) Wax gourd (B227) v1

Overview
NameBhi03G001600
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCysteine/Histidine-rich C1 domain family protein
Locationchr3: 43985001 .. 43986264 (-)
RNA-Seq ExpressionBhi03G001600
SyntenyBhi03G001600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGATGAAATTTTTAAAAACATAAAACAAAGGGATTTTCCTTTTTTGTTCTTTTAAACAAAAGAGAGACTAGAAGTCTTGGCTTCTCCAAAGTCTACACGACTCTTACTCTCTCCTTATAAGCCTGCTGGGGGAGGAACCCATTGATCGGATAAAAATGGCGCTGGTACCCGGAAACTCCACAAAGCAATATCAGTTATCCCAAGTCCATCCAAACCATTCTCTAATGCATCATTCCGACGACCGTGATTACACCTGCCACGGCTGCAAGACCTCCGGCACCGGCCCCCGATACCGATGCCACGCCTGCAACGTCGACGTCCATACATACTGCTCCGATTCCCCCCAGGAATTATCCTCCTTCCTCCACCACCATCGCCTCACTCTGGTTCCCCGTATGGACCATCGGTGCTGCGATATCTGCCGTGAATCTATCGACGGCATGCTTTACCGCTGCAACCGCTGCGACTTCGACGTCCACCCACTTTGTACTCAATTCCCTGAGCAGGTCCGCCACGTCATCGACGACCATCATAAGCTGACCCTCCGCAAGCTCTCCTCTGGGCGATGTTCTGTTTGCGAAATTGATTGTTCTTCTCTCTGGGTCTACGGCTGCAATGTTTGTGGAGTCAATATTCACCCCAAATGCCTGCTTAAGCCATACGGTTCGCCGACGAAATCTCGCAGTATACCTTACGCGCACGCGCCGCCGTGGGCGGCGGCGCATCAACCCAATGGTTATTATGTGAATCCAAATGGTTATGGTGGTGGCCATTTTTCTCACGGCGGAGTTCCGGCTCCTTGGGGATTTCCGAATCATCATTTTAATTATCCTCCTGGAGCTAATTATGGTGGACGTCCACCGTCGCCGTCAACTTGGGGATCAATATTTGGGGGTGGAATGTTTGCTCTGGTTCAAAATTTGACCGCAGGTGCTATTATTGAGTTTATTTTCGGTTCTGTTGGTGCTTAGTTCTTCTTGCAATGGTGTTTTATTGTTCATCATCTTCTTAAATTGAGAATTTTTCCTTTCCATTATGTTCTATTTAGCTGTGTCATTGGCTTATTGCTAAATTGCTGGTTTGGTACTGATAATTTTAAATTTTTAAAAAAAAAAAAAGAGAAAAAATGTAAGCAATAATTGCATTTATTATTGTACTGAAAATGCCCAGAAATAAGTCTGCCATGGTTGTACTGAAAAGGAACCACCATATGTTGCAAACTTGTGAGAAACAACACTATAAATATGTCTTTGAACCCTAC

mRNA sequence

ATGCGATGAAATTTTTAAAAACATAAAACAAAGGGATTTTCCTTTTTTGTTCTTTTAAACAAAAGAGAGACTAGAAGTCTTGGCTTCTCCAAAGTCTACACGACTCTTACTCTCTCCTTATAAGCCTGCTGGGGGAGGAACCCATTGATCGGATAAAAATGGCGCTGGTACCCGGAAACTCCACAAAGCAATATCAGTTATCCCAAGTCCATCCAAACCATTCTCTAATGCATCATTCCGACGACCGTGATTACACCTGCCACGGCTGCAAGACCTCCGGCACCGGCCCCCGATACCGATGCCACGCCTGCAACGTCGACGTCCATACATACTGCTCCGATTCCCCCCAGGAATTATCCTCCTTCCTCCACCACCATCGCCTCACTCTGGTTCCCCGTATGGACCATCGGTGCTGCGATATCTGCCGTGAATCTATCGACGGCATGCTTTACCGCTGCAACCGCTGCGACTTCGACGTCCACCCACTTTGTACTCAATTCCCTGAGCAGGTCCGCCACGTCATCGACGACCATCATAAGCTGACCCTCCGCAAGCTCTCCTCTGGGCGATGTTCTGTTTGCGAAATTGATTGTTCTTCTCTCTGGGTCTACGGCTGCAATGTTTGTGGAGTCAATATTCACCCCAAATGCCTGCTTAAGCCATACGGTTCGCCGACGAAATCTCGCAGTATACCTTACGCGCACGCGCCGCCGTGGGCGGCGGCGCATCAACCCAATGGTTATTATGTGAATCCAAATGGTTATGGTGGTGGCCATTTTTCTCACGGCGGAGTTCCGGCTCCTTGGGGATTTCCGAATCATCATTTTAATTATCCTCCTGGAGCTAATTATGGTGGACGTCCACCGTCGCCGTCAACTTGGGGATCAATATTTGGGGGTGGAATGTTTGCTCTGGTTCAAAATTTGACCGCAGGTGCTATTATTGAGTTTATTTTCGGTTCTGTTGGTGCTTAGTTCTTCTTGCAATGGTGTTTTATTGTTCATCATCTTCTTAAATTGAGAATTTTTCCTTTCCATTATGTTCTATTTAGCTGTGTCATTGGCTTATTGCTAAATTGCTGGTTTGGTACTGATAATTTTAAATTTTTAAAAAAAAAAAAAGAGAAAAAATGTAAGCAATAATTGCATTTATTATTGTACTGAAAATGCCCAGAAATAAGTCTGCCATGGTTGTACTGAAAAGGAACCACCATATGTTGCAAACTTGTGAGAAACAACACTATAAATATGTCTTTGAACCCTAC

Coding sequence (CDS)

ATGGCGCTGGTACCCGGAAACTCCACAAAGCAATATCAGTTATCCCAAGTCCATCCAAACCATTCTCTAATGCATCATTCCGACGACCGTGATTACACCTGCCACGGCTGCAAGACCTCCGGCACCGGCCCCCGATACCGATGCCACGCCTGCAACGTCGACGTCCATACATACTGCTCCGATTCCCCCCAGGAATTATCCTCCTTCCTCCACCACCATCGCCTCACTCTGGTTCCCCGTATGGACCATCGGTGCTGCGATATCTGCCGTGAATCTATCGACGGCATGCTTTACCGCTGCAACCGCTGCGACTTCGACGTCCACCCACTTTGTACTCAATTCCCTGAGCAGGTCCGCCACGTCATCGACGACCATCATAAGCTGACCCTCCGCAAGCTCTCCTCTGGGCGATGTTCTGTTTGCGAAATTGATTGTTCTTCTCTCTGGGTCTACGGCTGCAATGTTTGTGGAGTCAATATTCACCCCAAATGCCTGCTTAAGCCATACGGTTCGCCGACGAAATCTCGCAGTATACCTTACGCGCACGCGCCGCCGTGGGCGGCGGCGCATCAACCCAATGGTTATTATGTGAATCCAAATGGTTATGGTGGTGGCCATTTTTCTCACGGCGGAGTTCCGGCTCCTTGGGGATTTCCGAATCATCATTTTAATTATCCTCCTGGAGCTAATTATGGTGGACGTCCACCGTCGCCGTCAACTTGGGGATCAATATTTGGGGGTGGAATGTTTGCTCTGGTTCAAAATTTGACCGCAGGTGCTATTATTGAGTTTATTTTCGGTTCTGTTGGTGCTTAG

Protein sequence

MALVPGNSTKQYQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLHHHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRKLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPYAHAPPWAAAHQPNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANYGGRPPSPSTWGSIFGGGMFALVQNLTAGAIIEFIFGSVGA
Homology
BLAST of Bhi03G001600 vs. TAIR 10
Match: AT2G37820.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 161.8 bits (408), Expect = 7.9e-40
Identity = 101/272 (37.13%), Postives = 133/272 (48.90%), Query Frame = 0

Query: 21  HSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLH-HHRLTLV- 80
           H L   +   D+ C GCKT G+G  YRC  CN D+H YC+  P  L +F+H  H L+LV 
Sbjct: 18  HPLTEFNSIGDFICDGCKTYGSGKTYRCEPCNYDLHEYCATCPLTLPTFIHPQHELSLVV 77

Query: 81  -----PRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRKL 140
                 R + R CDIC ES++G+ YRC  C+FDVHPLCTQ P+ VRHV+   H L  R  
Sbjct: 78  RKQQSTRQNDRACDICDESVEGLFYRCKICEFDVHPLCTQLPQHVRHVLHPAHHLEFRPS 137

Query: 141 SSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLL---------KPYGSPTKSRSIPYAHAP 200
            +  C VC   C S W Y C +C  +IH +C+L         +   S TKSR +      
Sbjct: 138 GASTCMVCHGPCQS-WRYRCELCRFDIHMECILAVCNTSPSDQTDASGTKSRGLKQQGGQ 197

Query: 201 P-----WAAAHQPNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANY--GGRPPS 260
           P     W   H    Y   P G    +F++     P+ + N  FNY  G N   G  P +
Sbjct: 198 PSPPTQWQQPHFGYPYAYGPMGQQQPYFNY----HPYNYNN--FNYMNGYNMNPGQVPMA 257

Query: 261 PSTWGSIFGGG--MFALVQNLTAGAIIEFIFG 268
            ++  S    G  MF +   LT G +   IFG
Sbjct: 258 GASQNSTPSKGWRMFTIASKLTMGVVSSAIFG 282

BLAST of Bhi03G001600 vs. TAIR 10
Match: AT2G37800.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 129.0 bits (323), Expect = 5.7e-30
Identity = 89/280 (31.79%), Postives = 131/280 (46.79%), Query Frame = 0

Query: 7   NSTKQYQLSQVHPNHSLMHH----------SDDRDYTCHGCKTSGTGPRYRCHACNVDVH 66
           N+ K  ++S   P   ++ H              ++TC GCKT G G  YRC  C+ ++H
Sbjct: 128 NNKKTTKMSSSRPEQLVVQHFTHIHPLTKVDGYGEFTCDGCKTYGFGKTYRCTRCDYNLH 187

Query: 67  TYCSDSPQELSSFLH-HHRLTLVPR------MDHRCCDICRESIDGMLYRCNRCDFDVHP 126
            +C+  P  L++F+H  H L LV R       + R CDIC ES +G+ Y+C  C FDVHP
Sbjct: 188 DHCATCPSTLATFMHPQHELRLVFRGPEHTHQNKRMCDICDESAEGLYYQCEPCGFDVHP 247

Query: 127 LCTQFPEQVRHVIDDHHKLTLRKL-SSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKP 186
           LCTQ P+ VRHV    H L L +  +S  C VC     S W Y C  CG+++H +C+   
Sbjct: 248 LCTQLPQHVRHVPHPAHLLELSQWGASSICMVCRGAIRS-WRYKCGPCGLDVHMECISSS 307

Query: 187 YG-SPTKSRSIPYAHAPPWAAAHQPNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPP 246
              + TK +   +    P       + YY +   Y  G+ + G V    G+ N      P
Sbjct: 308 ASVAATKIQQRCFGSPQP---QFYHSEYYYHHAYYNHGYTNQGQV---HGYANQGQVQEP 367

Query: 247 GANYGGRPPSPSTWGSIFGGGMFALVQNLTAGAIIEFIFG 268
           G + G +              MF+++ +LT G +   I G
Sbjct: 368 GRSKGRK--------------MFSILMDLTVGVVCNIIAG 386

BLAST of Bhi03G001600 vs. TAIR 10
Match: AT2G37810.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.5e-25
Identity = 58/161 (36.02%), Postives = 82/161 (50.93%), Query Frame = 0

Query: 7   NSTKQYQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQEL 66
           +S +Q  +      H L    +  ++TC GCKT G G  YRC  C+  +H +C+  P  L
Sbjct: 4   SSVEQQVVQHFSHTHPLTKVDEYGEFTCGGCKTYGFGKTYRCAPCDYVLHDHCATCPFTL 63

Query: 67  SSFLH-HHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDH 126
            SF+H  H L L        C+IC   ++G+ Y C  C FDVHPLCTQ  + V +V    
Sbjct: 64  ISFMHPQHELRLFVNGSENMCNICHRLVEGVYYHCETCGFDVHPLCTQHHQHVSYVPHPA 123

Query: 127 HKLTLRKL-SSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCL 166
           H L L +  +S  C VC     S W Y C  C +++H +C+
Sbjct: 124 HLLELSQCGASNTCMVCRGAILS-WRYKCGPCMLDVHVECV 163

BLAST of Bhi03G001600 vs. TAIR 10
Match: AT5G22355.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 92.4 bits (228), Expect = 5.9e-19
Identity = 56/163 (34.36%), Postives = 78/163 (47.85%), Query Frame = 0

Query: 25  HHSDDRDY---TCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLHHHRLTLVPRM 84
           H  D R Y    C+ C+T  TG RY      +DV   C    + +    H H L    + 
Sbjct: 451 HSKDFRFYDVFRCYACRTKSTGFRYVSDWWVLDVR--CGSRSEPVIHDGHRHPLYYEHKK 510

Query: 85  DHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHH-KLTLRKLSSGR--C 144
           DH CCD C + IDG L  C+ CDFD+   CT  P+ V+H  D+H   L   + ++G+  C
Sbjct: 511 DH-CCDACYKEIDGYLLSCDTCDFDLDLHCTDLPKTVKHSCDNHPLSLCYGENATGKYWC 570

Query: 145 SVCEIDCS-SLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPY 181
            +CE +   S W Y C+ C V  H +C+L  +      R I Y
Sbjct: 571 DICEAETDPSKWFYTCSKCVVTAHIECVLGDFSRLMPGRIINY 610

BLAST of Bhi03G001600 vs. TAIR 10
Match: AT2G37780.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-18
Identity = 44/99 (44.44%), Postives = 54/99 (54.55%), Query Frame = 0

Query: 20  NHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLH--HHRLTL 79
           NH L   +    YTC GCK  G G  YRC  C+ D+H YC+  P  L +  H   H L+L
Sbjct: 13  NHLLTQVNGIGTYTCDGCKLYGEGRTYRCSDCDYDLHEYCATCPSILLNSCHGPDHELSL 72

Query: 80  V-PRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFP 116
               M  R C +CR SI GM Y+C +C F+ HPLCT  P
Sbjct: 73  FNGHMTERSCYVCRVSIQGMFYKCRQCSFEAHPLCTYAP 111

BLAST of Bhi03G001600 vs. ExPASy Swiss-Prot
Match: D3ZEY4 (Diacylglycerol kinase theta OS=Rattus norvegicus OX=10116 GN=Dgkq PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.3e-07
Identity = 48/173 (27.75%), Postives = 63/173 (36.42%), Query Frame = 0

Query: 14  LSQVHPNHSLMHHSDDRDYTCHGCKTSGTG-PRYRCHACNV--------DVHTYCSDSPQ 73
           +  V P HS    +  +   CH C     G   + C  CN          V T C+    
Sbjct: 48  IGSVAPGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKQVKTPCTSIAP 107

Query: 74  ELSSFLHHHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFP--------E 133
            L      H    +     + C +CR+S++   +RC  C+  VHP C  F         +
Sbjct: 108 SLVRVPVAHCFGSLGLYKRKFCVVCRKSLEVPAFRCEVCELHVHPDCVPFACSDCRQCHQ 167

Query: 134 QVRHVIDD-HHKLTLRKLSSG-RCSVCEIDCSSLWVYG---CNVCGVNIHPKC 165
             +H  D  HH      L SG RC VC   C S  V     C  CGV  H  C
Sbjct: 168 DGQHDYDTYHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSVC 220

BLAST of Bhi03G001600 vs. ExPASy Swiss-Prot
Match: Q6P5E8 (Diacylglycerol kinase theta OS=Mus musculus OX=10090 GN=Dgkq PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.5e-06
Identity = 47/168 (27.98%), Postives = 59/168 (35.12%), Query Frame = 0

Query: 19  PNHSLMHHSDDRDYTCHGCKTSGTG-PRYRCHACNV--------DVHTYCSDSPQELSSF 78
           P HS    +  +   CH C     G   + C  CN          V T C+     L   
Sbjct: 53  PGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKQVKTPCTSIAPSLVRV 112

Query: 79  LHHHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFP-EQVRHVIDD---- 138
              H    +     + C +CR+S++   +RC  C+  VHP C  F     R    D    
Sbjct: 113 PVAHCFGSLGLYKRKFCVVCRKSLEVPAFRCEVCELHVHPDCVPFACSDCRQCHQDGQQD 172

Query: 139 ----HHKLTLRKLSSG-RCSVCEIDCSSLWVYG---CNVCGVNIHPKC 165
               HH      L SG RC VC   C S  V     C  CGV  H  C
Sbjct: 173 YDTYHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSVC 220

BLAST of Bhi03G001600 vs. ExPASy Swiss-Prot
Match: Q7Y0F2 (Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405900 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.8e-04
Identity = 23/49 (46.94%), Postives = 26/49 (53.06%), Query Frame = 0

Query: 63  PQELSSFLHHHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLC 112
           P++L   LH H L L  R     CD C E  D   YRC  CDFD+HP C
Sbjct: 502 PEKLKHDLHDHELVLT-RCTTYGCDGCDEMGDSWSYRCKECDFDLHPKC 549

BLAST of Bhi03G001600 vs. ExPASy Swiss-Prot
Match: Q7Y0E8 (Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405500 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 5.2e-04
Identity = 18/53 (33.96%), Postives = 31/53 (58.49%), Query Frame = 0

Query: 114 FPEQVRHVIDDHHKLTLRKLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLL 167
           +P +++H + D H+L L + ++  C  C+ +  S W Y C  C  ++HPKC L
Sbjct: 490 WPGKLKHELHDEHELVLTRCTTYGCDGCD-EMGSSWSYRCRECDFDLHPKCAL 541

BLAST of Bhi03G001600 vs. NCBI nr
Match: XP_038883076.1 (diacylglycerol kinase theta-like [Benincasa hispida])

HSP 1 Score: 598.2 bits (1541), Expect = 3.5e-167
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0

Query: 1   MALVPGNSTKQYQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCS 60
           MALVPGNSTKQYQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCS
Sbjct: 1   MALVPGNSTKQYQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCS 60

Query: 61  DSPQELSSFLHHHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRH 120
           DSPQELSSFLHHHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRH
Sbjct: 61  DSPQELSSFLHHHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRH 120

Query: 121 VIDDHHKLTLRKLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPY 180
           VIDDHHKLTLRKLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPY
Sbjct: 121 VIDDHHKLTLRKLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPY 180

Query: 181 AHAPPWAAAHQPNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANYGGRPPSPST 240
           AHAPPWAAAHQPNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANYGGRPPSPST
Sbjct: 181 AHAPPWAAAHQPNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANYGGRPPSPST 240

Query: 241 WGSIFGGGMFALVQNLTAGAIIEFIFGSVGA 272
           WGSIFGGGMFALVQNLTAGAIIEFIFGSVGA
Sbjct: 241 WGSIFGGGMFALVQNLTAGAIIEFIFGSVGA 271

BLAST of Bhi03G001600 vs. NCBI nr
Match: XP_008448356.1 (PREDICTED: diacylglycerol kinase theta-like [Cucumis melo] >KAA0045158.1 diacylglycerol kinase theta-like [Cucumis melo var. makuwa] >TYK23580.1 diacylglycerol kinase theta-like [Cucumis melo var. makuwa])

HSP 1 Score: 382.9 bits (982), Expect = 2.3e-102
Identity = 175/260 (67.31%), Postives = 201/260 (77.31%), Query Frame = 0

Query: 12  YQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLH 71
           Y  S  HPNH L H+SDD+ YTCH C T  +G R+RC  C+VD+H +CS+SP+ELSSFLH
Sbjct: 8   YHFSHFHPNHPLTHYSDDQKYTCHICNTVDSGLRFRCQPCHVDIHKFCSNSPEELSSFLH 67

Query: 72  HHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLR 131
            H L L+P M+HR CDICRESI+GM YRCN CDFDVHPLCTQFPEQ+RH+ID  HKLTLR
Sbjct: 68  SHPLALIPPMNHRQCDICRESINGMFYRCNHCDFDVHPLCTQFPEQLRHLIDGRHKLTLR 127

Query: 132 KLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPYAHAPPWAAAHQ 191
           KLSSGRCSVCE DCSS WVYGC+VC VNIHP+C+L+PYGS T +R I Y  APPW  AHQ
Sbjct: 128 KLSSGRCSVCETDCSSFWVYGCDVCRVNIHPRCILRPYGSSTGTRGISYWQAPPWTTAHQ 187

Query: 192 PNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANYGGRPPSPSTWGSIFGGGMFA 251
                  PNGYGGG+F++GG PA  G+PN+H  Y  G NYGG+PPS   W  + G GMF 
Sbjct: 188 -------PNGYGGGYFAYGGGPANLGYPNNHVGYSYGGNYGGQPPSRPNWVPMLGAGMFG 247

Query: 252 LVQNLTAGAIIEFIFGSVGA 272
           LVQNLTAGAIIEFIFGS GA
Sbjct: 248 LVQNLTAGAIIEFIFGSFGA 260

BLAST of Bhi03G001600 vs. NCBI nr
Match: XP_004142189.1 (diacylglycerol kinase theta [Cucumis sativus] >KGN54292.1 hypothetical protein Csa_018032 [Cucumis sativus])

HSP 1 Score: 381.7 bits (979), Expect = 5.2e-102
Identity = 174/260 (66.92%), Postives = 206/260 (79.23%), Query Frame = 0

Query: 14  LSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHA-CNVDVHTYCSDSPQELSSFLHH 73
           +S  HP+H L H+SDD++YTCH CKT G+GPR+RC A C+VD+H YC+D P+ELSSFLH 
Sbjct: 11  ISHFHPDHPLTHYSDDQEYTCHICKTVGSGPRFRCQANCHVDIHLYCTDPPKELSSFLHS 70

Query: 74  HRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRK 133
           HRL L+ +M+HR C+ICRESI+GM YRCN CDFDVHPLCTQFPEQ+RH+ID  HKLT RK
Sbjct: 71  HRLALIHQMNHRRCNICRESINGMFYRCNHCDFDVHPLCTQFPEQLRHLIDGCHKLTFRK 130

Query: 134 LSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPYAHAPPWAAAHQP 193
           LSSGRCS+CE DCSS WVYGC+VC VNIHPKC+LKPYGSPT +R IPY  AP W  A   
Sbjct: 131 LSSGRCSICEEDCSSFWVYGCDVCRVNIHPKCILKPYGSPTGTRGIPYCQAPQWTTA--- 190

Query: 194 NGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGA-NYGGRPPSPSTWGSIFGGGMFA 253
                +P+GYGGG+FS+GG    WG+PNHH  YP G  NYGG+PPS  +W  + G GMF 
Sbjct: 191 ----PHPHGYGGGYFSYGGGQPNWGYPNHHVGYPHGGNNYGGQPPSRPSWVPMLGAGMFG 250

Query: 254 LVQNLTAGAIIEFIFGSVGA 272
           +VQNLTAGAI+EFIFGS GA
Sbjct: 251 VVQNLTAGAILEFIFGSFGA 263

BLAST of Bhi03G001600 vs. NCBI nr
Match: XP_038888649.1 (uncharacterized protein LOC120078450 [Benincasa hispida])

HSP 1 Score: 313.5 bits (802), Expect = 1.7e-81
Identity = 145/167 (86.83%), Postives = 153/167 (91.62%), Query Frame = 0

Query: 105 FDVHPLCTQFPEQVRHVIDDHHKLTLRKLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKC 164
           FDVHPLCTQFPEQVRHVIDDH+KLTLR LSSG+CSVCEIDCSSLWVY  NVCGVNIHPKC
Sbjct: 31  FDVHPLCTQFPEQVRHVIDDHNKLTLRNLSSGQCSVCEIDCSSLWVYSFNVCGVNIHPKC 90

Query: 165 LLKPYGSPTKSRSIPYAHAPPWAAAHQPNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFN 224
           LLKPYGSPTKSR I +AHAPPWAAAH+PNGY+VNPNGY GGHFS+GGVPAPWGFPNH+F+
Sbjct: 91  LLKPYGSPTKSRRILHAHAPPWAAAHKPNGYHVNPNGYDGGHFSYGGVPAPWGFPNHNFS 150

Query: 225 YPPGANYGGRPPSPSTWGSIFGGGMFALVQNLTAGAIIEFIFGSVGA 272
           Y PGANYGG  PSP  WGSIFGGGMFALVQNL AGAIIEFI GS GA
Sbjct: 151 YAPGANYGGHLPSPPIWGSIFGGGMFALVQNLAAGAIIEFISGSAGA 197

BLAST of Bhi03G001600 vs. NCBI nr
Match: XP_022931897.1 (uncharacterized protein LOC111438174 isoform X2 [Cucurbita moschata])

HSP 1 Score: 290.4 bits (742), Expect = 1.6e-74
Identity = 161/334 (48.20%), Postives = 186/334 (55.69%), Query Frame = 0

Query: 14  LSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLHHH 73
           +S VHPNH L ++SD   YTC GCKTSG G R+RCH C+VDVH YC D P +L+SFLH H
Sbjct: 9   VSHVHPNHPLNYYSDPHVYTCQGCKTSGIGNRFRCHICSVDVHIYCLDCPPQLASFLHTH 68

Query: 74  RLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRKL 133
            L L+P MDHR CD+CRE I+G+ YRC+ CDFDVHPLCTQ PEQ+ H ID  HKL L KL
Sbjct: 69  SLALLPCMDHRSCDVCRERIEGLFYRCDSCDFDVHPLCTQLPEQLHHGIDKRHKLRLHKL 128

Query: 134 SSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYG---------SPTKSRSIPYAHAP 193
           SSGRCSVCE DCS LWVY CNVC VNIHP+C+LKPYG          P+ SRSIPYAH P
Sbjct: 129 SSGRCSVCESDCSYLWVYSCNVCRVNIHPQCILKPYGPRRSENPAVPPSDSRSIPYAHEP 188

Query: 194 P-----------------WAAAHQPNGYY-------------------------VNPNGY 253
                             W   ++PN Y                            PN Y
Sbjct: 189 SGFGGYGYAGGGGGHSSHWRFPNEPNSYRSVQFPHNNPDHSSRWRFPNFHSSYPTQPNSY 248

Query: 254 GG--------GHFSHGGVPAPWGFPNHHFNYPPGAN-----------------YGGRPP- 269
           G         GH SHG       FPN H +Y    N                 YGG+   
Sbjct: 249 GSVQFSPNNPGHSSHG------RFPNFHSSYLTQPNSYGSVQFPQNNPCPRPIYGGQTSS 308

BLAST of Bhi03G001600 vs. ExPASy TrEMBL
Match: A0A5A7TQK1 (Diacylglycerol kinase theta-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00880 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.1e-102
Identity = 175/260 (67.31%), Postives = 201/260 (77.31%), Query Frame = 0

Query: 12  YQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLH 71
           Y  S  HPNH L H+SDD+ YTCH C T  +G R+RC  C+VD+H +CS+SP+ELSSFLH
Sbjct: 8   YHFSHFHPNHPLTHYSDDQKYTCHICNTVDSGLRFRCQPCHVDIHKFCSNSPEELSSFLH 67

Query: 72  HHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLR 131
            H L L+P M+HR CDICRESI+GM YRCN CDFDVHPLCTQFPEQ+RH+ID  HKLTLR
Sbjct: 68  SHPLALIPPMNHRQCDICRESINGMFYRCNHCDFDVHPLCTQFPEQLRHLIDGRHKLTLR 127

Query: 132 KLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPYAHAPPWAAAHQ 191
           KLSSGRCSVCE DCSS WVYGC+VC VNIHP+C+L+PYGS T +R I Y  APPW  AHQ
Sbjct: 128 KLSSGRCSVCETDCSSFWVYGCDVCRVNIHPRCILRPYGSSTGTRGISYWQAPPWTTAHQ 187

Query: 192 PNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANYGGRPPSPSTWGSIFGGGMFA 251
                  PNGYGGG+F++GG PA  G+PN+H  Y  G NYGG+PPS   W  + G GMF 
Sbjct: 188 -------PNGYGGGYFAYGGGPANLGYPNNHVGYSYGGNYGGQPPSRPNWVPMLGAGMFG 247

Query: 252 LVQNLTAGAIIEFIFGSVGA 272
           LVQNLTAGAIIEFIFGS GA
Sbjct: 248 LVQNLTAGAIIEFIFGSFGA 260

BLAST of Bhi03G001600 vs. ExPASy TrEMBL
Match: A0A1S3BK43 (diacylglycerol kinase theta-like OS=Cucumis melo OX=3656 GN=LOC103490570 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.1e-102
Identity = 175/260 (67.31%), Postives = 201/260 (77.31%), Query Frame = 0

Query: 12  YQLSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLH 71
           Y  S  HPNH L H+SDD+ YTCH C T  +G R+RC  C+VD+H +CS+SP+ELSSFLH
Sbjct: 8   YHFSHFHPNHPLTHYSDDQKYTCHICNTVDSGLRFRCQPCHVDIHKFCSNSPEELSSFLH 67

Query: 72  HHRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLR 131
            H L L+P M+HR CDICRESI+GM YRCN CDFDVHPLCTQFPEQ+RH+ID  HKLTLR
Sbjct: 68  SHPLALIPPMNHRQCDICRESINGMFYRCNHCDFDVHPLCTQFPEQLRHLIDGRHKLTLR 127

Query: 132 KLSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPYAHAPPWAAAHQ 191
           KLSSGRCSVCE DCSS WVYGC+VC VNIHP+C+L+PYGS T +R I Y  APPW  AHQ
Sbjct: 128 KLSSGRCSVCETDCSSFWVYGCDVCRVNIHPRCILRPYGSSTGTRGISYWQAPPWTTAHQ 187

Query: 192 PNGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGANYGGRPPSPSTWGSIFGGGMFA 251
                  PNGYGGG+F++GG PA  G+PN+H  Y  G NYGG+PPS   W  + G GMF 
Sbjct: 188 -------PNGYGGGYFAYGGGPANLGYPNNHVGYSYGGNYGGQPPSRPNWVPMLGAGMFG 247

Query: 252 LVQNLTAGAIIEFIFGSVGA 272
           LVQNLTAGAIIEFIFGS GA
Sbjct: 248 LVQNLTAGAIIEFIFGSFGA 260

BLAST of Bhi03G001600 vs. ExPASy TrEMBL
Match: A0A0A0L121 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G297435 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.5e-102
Identity = 174/260 (66.92%), Postives = 206/260 (79.23%), Query Frame = 0

Query: 14  LSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHA-CNVDVHTYCSDSPQELSSFLHH 73
           +S  HP+H L H+SDD++YTCH CKT G+GPR+RC A C+VD+H YC+D P+ELSSFLH 
Sbjct: 11  ISHFHPDHPLTHYSDDQEYTCHICKTVGSGPRFRCQANCHVDIHLYCTDPPKELSSFLHS 70

Query: 74  HRLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRK 133
           HRL L+ +M+HR C+ICRESI+GM YRCN CDFDVHPLCTQFPEQ+RH+ID  HKLT RK
Sbjct: 71  HRLALIHQMNHRRCNICRESINGMFYRCNHCDFDVHPLCTQFPEQLRHLIDGCHKLTFRK 130

Query: 134 LSSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYGSPTKSRSIPYAHAPPWAAAHQP 193
           LSSGRCS+CE DCSS WVYGC+VC VNIHPKC+LKPYGSPT +R IPY  AP W  A   
Sbjct: 131 LSSGRCSICEEDCSSFWVYGCDVCRVNIHPKCILKPYGSPTGTRGIPYCQAPQWTTA--- 190

Query: 194 NGYYVNPNGYGGGHFSHGGVPAPWGFPNHHFNYPPGA-NYGGRPPSPSTWGSIFGGGMFA 253
                +P+GYGGG+FS+GG    WG+PNHH  YP G  NYGG+PPS  +W  + G GMF 
Sbjct: 191 ----PHPHGYGGGYFSYGGGQPNWGYPNHHVGYPHGGNNYGGQPPSRPSWVPMLGAGMFG 250

Query: 254 LVQNLTAGAIIEFIFGSVGA 272
           +VQNLTAGAI+EFIFGS GA
Sbjct: 251 VVQNLTAGAILEFIFGSFGA 263

BLAST of Bhi03G001600 vs. ExPASy TrEMBL
Match: A0A6J1EV38 (uncharacterized protein LOC111438174 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111438174 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 7.6e-75
Identity = 161/334 (48.20%), Postives = 186/334 (55.69%), Query Frame = 0

Query: 14  LSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLHHH 73
           +S VHPNH L ++SD   YTC GCKTSG G R+RCH C+VDVH YC D P +L+SFLH H
Sbjct: 9   VSHVHPNHPLNYYSDPHVYTCQGCKTSGIGNRFRCHICSVDVHIYCLDCPPQLASFLHTH 68

Query: 74  RLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRKL 133
            L L+P MDHR CD+CRE I+G+ YRC+ CDFDVHPLCTQ PEQ+ H ID  HKL L KL
Sbjct: 69  SLALLPCMDHRSCDVCRERIEGLFYRCDSCDFDVHPLCTQLPEQLHHGIDKRHKLRLHKL 128

Query: 134 SSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYG---------SPTKSRSIPYAHAP 193
           SSGRCSVCE DCS LWVY CNVC VNIHP+C+LKPYG          P+ SRSIPYAH P
Sbjct: 129 SSGRCSVCESDCSYLWVYSCNVCRVNIHPQCILKPYGPRRSENPAVPPSDSRSIPYAHEP 188

Query: 194 P-----------------WAAAHQPNGYY-------------------------VNPNGY 253
                             W   ++PN Y                            PN Y
Sbjct: 189 SGFGGYGYAGGGGGHSSHWRFPNEPNSYRSVQFPHNNPDHSSRWRFPNFHSSYPTQPNSY 248

Query: 254 GG--------GHFSHGGVPAPWGFPNHHFNYPPGAN-----------------YGGRPP- 269
           G         GH SHG       FPN H +Y    N                 YGG+   
Sbjct: 249 GSVQFSPNNPGHSSHG------RFPNFHSSYLTQPNSYGSVQFPQNNPCPRPIYGGQTSS 308

BLAST of Bhi03G001600 vs. ExPASy TrEMBL
Match: A0A6J1EUW5 (uncharacterized protein LOC111438174 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438174 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.8e-71
Identity = 161/354 (45.48%), Postives = 187/354 (52.82%), Query Frame = 0

Query: 14  LSQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQELSSFLHHH 73
           +S VHPNH L ++SD   YTC GCKTSG G R+RCH C+VDVH YC D P +L+SFLH H
Sbjct: 9   VSHVHPNHPLNYYSDPHVYTCQGCKTSGIGNRFRCHICSVDVHIYCLDCPPQLASFLHTH 68

Query: 74  RLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRKL 133
            L L+P MDHR CD+CRE I+G+ YRC+ CDFDVHPLCTQ PEQ+ H ID  HKL L KL
Sbjct: 69  SLALLPCMDHRSCDVCRERIEGLFYRCDSCDFDVHPLCTQLPEQLHHGIDKRHKLRLHKL 128

Query: 134 SSGRCSVCEIDCSSLWVYGCNVCGVNIHPKCLLKPYG---------SPTKSRSIPYAHAP 193
           SSGRCSVCE DCS LWVY CNVC VNIHP+C+LKPYG          P+ SRSIPYAH P
Sbjct: 129 SSGRCSVCESDCSYLWVYSCNVCRVNIHPQCILKPYGPRRSENPAVPPSDSRSIPYAHEP 188

Query: 194 -------------------------------PWAAAHQ--PN---GYYVNPNGYGGGHFS 253
                                          P  ++H   PN    Y   PN YG   F 
Sbjct: 189 SGFGGYGYAGGGGGHSSHWRFPNSVQFPHNNPDHSSHGRFPNFHSSYPTQPNSYGSVQFP 248

Query: 254 HGGV--PAPWGFPNHHFNYP---------------PGANYGGRPP--------------- 269
           H      +   FPN H +YP               PG +  GR P               
Sbjct: 249 HNNPDHSSRGRFPNFHSSYPTQPNSYGSVQFSPNNPGHSSHGRFPNFHSSYLTQPNSYGS 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G37820.17.9e-4037.13Cysteine/Histidine-rich C1 domain family protein [more]
AT2G37800.15.7e-3031.79Cysteine/Histidine-rich C1 domain family protein [more]
AT2G37810.12.5e-2536.02Cysteine/Histidine-rich C1 domain family protein [more]
AT5G22355.15.9e-1934.36Cysteine/Histidine-rich C1 domain family protein [more]
AT2G37780.11.0e-1844.44Cysteine/Histidine-rich C1 domain family protein [more]
Match NameE-valueIdentityDescription
D3ZEY42.3e-0727.75Diacylglycerol kinase theta OS=Rattus norvegicus OX=10116 GN=Dgkq PE=1 SV=1[more]
Q6P5E81.5e-0627.98Diacylglycerol kinase theta OS=Mus musculus OX=10090 GN=Dgkq PE=1 SV=1[more]
Q7Y0F21.8e-0446.94Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Q7Y0E85.2e-0433.96Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Match NameE-valueIdentityDescription
XP_038883076.13.5e-167100.00diacylglycerol kinase theta-like [Benincasa hispida][more]
XP_008448356.12.3e-10267.31PREDICTED: diacylglycerol kinase theta-like [Cucumis melo] >KAA0045158.1 diacylg... [more]
XP_004142189.15.2e-10266.92diacylglycerol kinase theta [Cucumis sativus] >KGN54292.1 hypothetical protein C... [more]
XP_038888649.11.7e-8186.83uncharacterized protein LOC120078450 [Benincasa hispida][more]
XP_022931897.11.6e-7448.20uncharacterized protein LOC111438174 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A5A7TQK11.1e-10267.31Diacylglycerol kinase theta-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BK431.1e-10267.31diacylglycerol kinase theta-like OS=Cucumis melo OX=3656 GN=LOC103490570 PE=4 SV... [more]
A0A0A0L1212.5e-10266.92Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A6J1EV387.6e-7548.20uncharacterized protein LOC111438174 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1EUW51.8e-7145.48uncharacterized protein LOC111438174 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004146DC1PFAMPF03107C1_2coord: 17..60
e-value: 1.4E-6
score: 28.5
coord: 69..111
e-value: 3.1E-9
score: 37.0
NoneNo IPR availablePANTHERPTHR47841DIACYLGLYCEROL KINASE THETA-LIKE-RELATEDcoord: 16..268
NoneNo IPR availablePANTHERPTHR47841:SF7DIACYLGLYCEROL KINASE THETA-LIKEcoord: 16..268
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 44..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001600Bhi03M001600mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction