Bhi03G001383 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G001383
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Eukaryotic aspartyl protease family protein
Location	chr3: 37537847 .. 37539229 (-)
RNA-Seq Expression	Bhi03G001383
Synteny	Bhi03G001383

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTTTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTCCCACTTCATCTTCTTCCTCCATCTCCACTATCACACTCCCCCTCACTGCTTTCCCTTCCATTCCACTTACAGATGATCCATTGAAAATTATCAATTATCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAGAACCCACAAACGAAATCAATCCAGAATGTCTCTCTGTTCTCTCGTAGCTATGGAGCTTATTCAATCACACTCGCCTTCGGAACTCCACCGCAGAATTTATCGTTCGTCTTCGATACTGGAAGTAGTCTTGTTTGGTTCCCCTGCACCGCCGGTTATCGTTGTTCTAATTGTTCGTTTCCGAATGTTGATGCTGCAACGATTCCAAAATTTGTTCCCAAATTATCTTCCTCTGCGAAGATTATTGGTTGTCGAAATCCAAAATGTGCTTGGATTTTTGGCCCTAATTTGAACTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAATTGTTCCGGTTCTTGTCCTGGTTATGGAATTCTGTACGGCTCTGGCGCAACCGCTGGATTTCTCCTTTCTGAAACGCTTGATTTCCCCAAGAAAGGAGTGCCGGATTTTCTCGTTGGTTGTTCCGTCTCGTCCGTTCATCAACCAGCCGGCATTGCCGGATTCGGCCGCGCTCCTGAATCGTTGCCCTCGCAAATGCGATTGAAACGTTTCTCCTATTGCCTCGTTTCTCGCGGGTTCGACGACTCGCCGGTGAGTAGTCCTCTAGTACTGGATTCCGGTTCGGAATCCGACGATTCGAAGACTGAGAGTTTCATTTACGCACCGTTCCGAGAGAATCCATCAGGATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCCTCATCGGTGGAAAGCCGGTGAAGATCCCGTACAAGTATCTCATGCCGGATTCCGCCGGGAAGGGCGGCGCGATCATCGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCAATTTTCGAAGCAGTAGCGGGAGAATTGGAGAAGCAGCTGGTGAAATATCCTCGAACTAAGAGCGTTGAAGTGCAGTCCGGTTTGCGGCCGTGCTTCGATATTTCCAAGGAGGTATCGGTGGAGTTTCCGGAACTGGTTTTAAAGTTTAAAGGCGGAGCGAAGCTGAGTTTGCCGCCGGTGAATTACTTGGCGTTGGTGACGGATGCCGGCGTGGTATGCTTGACGATGATGACGGATGTAGGCGTCGTGGGTGGCGGCGCGGGGCCGGCGATTATATTTGGGGCGTTTCAGCAGCAGAATGTATTGGTGGAGTATGATTTGGCGAGGAACAGAATCGGATTTCGGAAGCAGAGATGCACGTGA

mRNA sequence

ATGGAGTTTTTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTCCCACTTCATCTTCTTCCTCCATCTCCACTATCACACTCCCCCTCACTGCTTTCCCTTCCATTCCACTTACAGATGATCCATTGAAAATTATCAATTATCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAGAACCCACAAACGAAATCAATCCAGAATGTCTCTCTGTTCTCTCGTAGCTATGGAGCTTATTCAATCACACTCGCCTTCGGAACTCCACCGCAGAATTTATCGTTCGTCTTCGATACTGGAAGTAGTCTTGTTTGGTTCCCCTGCACCGCCGGTTATCGTTGTTCTAATTGTTCGTTTCCGAATGTTGATGCTGCAACGATTCCAAAATTTGTTCCCAAATTATCTTCCTCTGCGAAGATTATTGGTTGTCGAAATCCAAAATGTGCTTGGATTTTTGGCCCTAATTTGAACTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAATTGTTCCGGTTCTTGTCCTGGTTATGGAATTCTGTACGGCTCTGGCGCAACCGCTGGATTTCTCCTTTCTGAAACGCTTGATTTCCCCAAGAAAGGAGTGCCGGATTTTCTCGTTGGTTGTTCCGTCTCGTCCGTTCATCAACCAGCCGGCATTGCCGGATTCGGCCGCGCTCCTGAATCGTTGCCCTCGCAAATGCGATTGAAACGTTTCTCCTATTGCCTCGTTTCTCGCGGGTTCGACGACTCGCCGGTGAGTAGTCCTCTAGTACTGGATTCCGGTTCGGAATCCGACGATTCGAAGACTGAGAGTTTCATTTACGCACCGTTCCGAGAGAATCCATCAGGATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCCTCATCGGTGGAAAGCCGGTGAAGATCCCGTACAAGTATCTCATGCCGGATTCCGCCGGGAAGGGCGGCGCGATCATCGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCAATTTTCGAAGCAGTAGCGGGAGAATTGGAGAAGCAGCTGGTGAAATATCCTCGAACTAAGAGCGTTGAAGTGCAGTCCGGTTTGCGGCCGTGCTTCGATATTTCCAAGGAGGTATCGGTGGAGTTTCCGGAACTGGTTTTAAAGTTTAAAGGCGGAGCGAAGCTGAGTTTGCCGCCGGTGAATTACTTGGCGTTGGTGACGGATGCCGGCGTGGTATGCTTGACGATGATGACGGATGTAGGCGTCGTGGGTGGCGGCGCGGGGCCGGCGATTATATTTGGGGCGTTTCAGCAGCAGAATGTATTGGTGGAGTATGATTTGGCGAGGAACAGAATCGGATTTCGGAAGCAGAGATGCACGTGA

Coding sequence (CDS)

ATGGAGTTTTTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTCCCACTTCATCTTCTTCCTCCATCTCCACTATCACACTCCCCCTCACTGCTTTCCCTTCCATTCCACTTACAGATGATCCATTGAAAATTATCAATTATCTTCTCTCTGCTTCACTCAACAGAGCTCAACATCTCAAGAACCCACAAACGAAATCAATCCAGAATGTCTCTCTGTTCTCTCGTAGCTATGGAGCTTATTCAATCACACTCGCCTTCGGAACTCCACCGCAGAATTTATCGTTCGTCTTCGATACTGGAAGTAGTCTTGTTTGGTTCCCCTGCACCGCCGGTTATCGTTGTTCTAATTGTTCGTTTCCGAATGTTGATGCTGCAACGATTCCAAAATTTGTTCCCAAATTATCTTCCTCTGCGAAGATTATTGGTTGTCGAAATCCAAAATGTGCTTGGATTTTTGGCCCTAATTTGAACTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAATTGTTCCGGTTCTTGTCCTGGTTATGGAATTCTGTACGGCTCTGGCGCAACCGCTGGATTTCTCCTTTCTGAAACGCTTGATTTCCCCAAGAAAGGAGTGCCGGATTTTCTCGTTGGTTGTTCCGTCTCGTCCGTTCATCAACCAGCCGGCATTGCCGGATTCGGCCGCGCTCCTGAATCGTTGCCCTCGCAAATGCGATTGAAACGTTTCTCCTATTGCCTCGTTTCTCGCGGGTTCGACGACTCGCCGGTGAGTAGTCCTCTAGTACTGGATTCCGGTTCGGAATCCGACGATTCGAAGACTGAGAGTTTCATTTACGCACCGTTCCGAGAGAATCCATCAGGATCCAACGCCGCATTTCGAGAGTACTATTACCTTAGTCTTCGGAGAATCCTCATCGGTGGAAAGCCGGTGAAGATCCCGTACAAGTATCTCATGCCGGATTCCGCCGGGAAGGGCGGCGCGATCATCGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCAATTTTCGAAGCAGTAGCGGGAGAATTGGAGAAGCAGCTGGTGAAATATCCTCGAACTAAGAGCGTTGAAGTGCAGTCCGGTTTGCGGCCGTGCTTCGATATTTCCAAGGAGGTATCGGTGGAGTTTCCGGAACTGGTTTTAAAGTTTAAAGGCGGAGCGAAGCTGAGTTTGCCGCCGGTGAATTACTTGGCGTTGGTGACGGATGCCGGCGTGGTATGCTTGACGATGATGACGGATGTAGGCGTCGTGGGTGGCGGCGCGGGGCCGGCGATTATATTTGGGGCGTTTCAGCAGCAGAATGTATTGGTGGAGTATGATTTGGCGAGGAACAGAATCGGATTTCGGAAGCAGAGATGCACGTGA

Protein sequence

MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQHLKNPQTKSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRLKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT

Homology

[Close]

BLAST of Bhi03G001383 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 476.1 bits (1224), Expect = 3.2e-134
Identity = 248/471 (52.65%), Postives = 320/471 (67.94%), Query Frame = 0


Query: 8   FLFSIFLLLPTSSSSSISTITLPLTAFP-SIPLTDDPLKIINYLLSASLNRAQHLKN--- 67
           F F IFL       S +S + LPL+ F  S     DP   +  L  +S+ RA  LK+   
Sbjct: 7   FFFLIFL-------SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTS 66

Query: 68  --PQTKSIQNVS----------LFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCT 127
             P   ++ + +          L ++SYG YS++L+FGTP Q + FVFDTGSSLVW PCT
Sbjct: 67  IKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCT 126

Query: 128 AGYRCSNCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRN 187
           + Y CS C F  +D   IP+F+PK SSS+KIIGC++PKC +++GPN+  +CR C+P +RN
Sbjct: 127 SRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRN 186

Query: 188 CSGSCPGYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPES 247
           C+  CP Y + YG G+TAG L++E LDFP   VPDF+VGCS+ S  QPAGIAGFGR P S
Sbjct: 187 CTVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVS 246

Query: 248 LPSQMRLKRFSYCLVSRGFDDSPVSSPLVLDSGS-ESDDSKTESFIYAPFRENPSGSNAA 307
           LPSQM LKRFS+CLVSR FDD+ V++ L LD+GS  +  SKT    Y PFR+NP+ SN A
Sbjct: 247 LPSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKA 306

Query: 308 FREYYYLSLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELE 367
           F EYYYL+LRRI +G K VKIPYKYL P + G GG+I+DSGSTFTF+++P+FE VA E  
Sbjct: 307 FLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFA 366

Query: 368 KQLVKYPRTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAG 427
            Q+  Y R K +E ++GL PCF+IS +  V  PEL+ +FKGGAKL LP  NY   V +  
Sbjct: 367 SQMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNTD 426

Query: 428 VVCLTMMTDVGV-VGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
            VCLT+++D  V   GG GPAII G+FQQQN LVEYDL  +R GF K++C+
Sbjct: 427 TVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of Bhi03G001383 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-49
Identity = 155/488 (31.76%), Postives = 235/488 (48.16%), Query Frame = 0


Query: 23  SISTITLPLTAFPSIPL-----TDDPLKIINYLLSASLNRAQHLKNPQTKSIQNVSLFSR 82
           S+S+++ PL    S  L     +  PL ++    S+S + A+  ++   +  Q +SL   
Sbjct: 21  SVSSLSTPLLLHLSHSLSTSKHSSSPLHLLK--SSSSRSSARFRRHHHKQQQQQLSLPIS 80

Query: 83  SYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFVP-KL 142
           S   Y I+L+ G+    +S   DTGS LVWFPC   + C  C     ++  +P   P  L
Sbjct: 81  SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILC-----ESKPLPPSPPSSL 140

Query: 143 SSSAKIIGCRNPKCAWIFG--PNLN----SRCRNCNPKSRNCSGS---CPGYGILYGSGA 202
           SSSA  + C +P C+      P+ +    S C     ++ +C+ S   CP +   YG G+
Sbjct: 141 SSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGS 200

Query: 203 TAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRL------KRF 262
               L S++L  P   V +F  GC+ +++ +P G+AGFGR   SLP+Q+ +        F
Sbjct: 201 LVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSF 260

Query: 263 SYCLVSRGFDDSPV--SSPLVL--------------DSGSESDDSKTE--SFIYAPFREN 322
           SYCLVS  FD   V   SPL+L              D   + DD K +   F++    EN
Sbjct: 261 SYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLEN 320

Query: 323 PSGSNAAFREYYYLSLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFE 382
           P         +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + 
Sbjct: 321 PK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYN 380

Query: 383 AVAGELEKQLVK-YPRTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGG-AKLSLPPVN 442
           +V  E + ++ + + R   VE  SG+ PC+ +++ V V  P LVL F G  + ++LP  N
Sbjct: 381 SVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKV--PALVLHFAGNRSSVTLPRRN 440

Query: 443 YLALVTDAG--------VVCLTMMT--DVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNR 460
           Y     D G        + CL +M   D   + GG G   I G +QQQ   V YDL   R
Sbjct: 441 YFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTG--AILGNYQQQGFEVVYDLLNRR 491

[Close]

BLAST of Bhi03G001383 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 192.2 bits (487), Expect = 9.2e-49
Identity = 150/471 (31.85%), Postives = 213/471 (45.22%), Query Frame = 0


Query: 17  PTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQHLKNPQTKSIQNVSLFS 76
           P+SSSSS   +TL  ++  S+P             +      + +K P +     +    
Sbjct: 32  PSSSSSSFLVLTLTKSSV-SLP-------------TPKSQTQERIKKPLSSVDVVMEPLR 91

Query: 77  RSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPC-TAGYRCSNC-SFPNVDAATIPKFVP 136
                Y ITL  GTPPQ +    DTGS L W PC    + C  C    N D  +   F P
Sbjct: 92  EVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKNNDLKSPSVFSP 151

Query: 137 KLSSSAKIIGCRNPKCAWI------FGPNLNSRCRNCNPKSRNCSGSCPGYGILYGSGA- 196
             SS++    C +  C  I      F P   + C         C   CP +   YG G  
Sbjct: 152 LHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTCVRPCPSFAYTYGEGGL 211

Query: 197 TAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRL--KRFSYCL 256
            +G L  + L    + VP F  GC  S+  +P GIAGFGR   SLPSQ+    K FS+C 
Sbjct: 212 ISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRGLLSLPSQLGFLEKGFSHCF 271

Query: 257 VSRGFDDSP-VSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLRRILI 316
           +   F ++P +SSPL+L + + S  + T+S  + P    P   N+     YY+ L  I I
Sbjct: 272 LPFKFVNNPNISSPLILGASALS-INLTDSLQFTPMLNTPMYPNS-----YYIGLESITI 331

Query: 317 GGK--PVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTKSV 376
           G    P ++P      DS G GG ++DSG+T+T L +P +  +   L+   + YPR    
Sbjct: 332 GTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQ-STITYPRATET 391

Query: 377 EVQSGLRPCF----------DISKEVSVEFPELVLKFKGGAKLSLPPVN---YLALVTDA 436
           E ++G   C+           +  +V + FP +   F   A L LP  N    ++  +D 
Sbjct: 392 ESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNATLLLPQGNSFYAMSAPSDG 451

Query: 437 GVV-CLTMMTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRC 460
            VV CL       +  G  GPA +FG+FQQQNV V YDL + RIGF+   C
Sbjct: 452 SVVQCLLFQ---NMEDGDYGPAGVFGSFQQQNVKVVYDLEKERIGFQAMDC 478

[Close]

BLAST of Bhi03G001383 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 158.7 bits (400), Expect = 1.1e-38
Identity = 122/398 (30.65%), Postives = 183/398 (45.98%), Query Frame = 0


Query: 80  GAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRC--SNCSFPNVDAATIPKFVPKLS 139
           G Y + +  G+PP++ S + DTGS L W  C   Y C   N +F          + PK S
Sbjct: 168 GEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQNGAF----------YDPKAS 227

Query: 140 SSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYGILYGSGATAGFLLSE-- 199
           +S K I C + +C  +  P+    C++ N        SCP Y     S  T G    E  
Sbjct: 228 ASYKNITCNDQRCNLVSSPDPPMPCKSDNQ-------SCPYYYWYGDSSNTTGDFAVETF 287

Query: 200 TLDFPKKG-------VPDFLVGC---SVSSVHQPAGIAGFGRAPESLPSQMRL---KRFS 259
           T++    G       V + + GC   +    H  AG+ G GR P S  SQ++      FS
Sbjct: 288 TVNLTTNGGSSELYNVENMMFGCGHWNRGLFHGAAGLLGLGRGPLSFSSQLQSLYGHSFS 347

Query: 260 YCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLRRI 319
           YCLV R   D+ VSS L+       +D    S     F    +G       +YY+ ++ I
Sbjct: 348 YCLVDRN-SDTNVSSKLIF-----GEDKDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSI 407

Query: 320 LIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGEL-EKQLVKYPRTKS 379
           L+ G+ + IP +     S G GG IIDSG+T ++  +P +E +  ++ EK   KYP  + 
Sbjct: 408 LVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRD 467

Query: 380 VEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTDVG 439
             +   L PCF++S   +V+ PEL + F  GA  + P  N    + +  +VCL M+    
Sbjct: 468 FPI---LDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLNE-DLVCLAML---- 527

Query: 440 VVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRC 460
             G       I G +QQQN  + YD  R+R+G+   +C
Sbjct: 528 --GTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

[Close]

BLAST of Bhi03G001383 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 156.8 bits (395), Expect = 4.3e-38
Identity = 126/419 (30.07%), Postives = 181/419 (43.20%), Query Frame = 0


Query: 47  INYLLSASLNRAQHLKNPQTKSIQN--VSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSS 106
           IN +  A L     +   + + I+   +S  ++  G Y   +  G P + +  V DTGS 
Sbjct: 111 INNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSD 170

Query: 107 LVWFPCTAGYRCSNCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRN 166
           + W  CT    C++C        T P F P  SSS + + C  P+C  +      S CRN
Sbjct: 171 VNWLQCTP---CADCYH-----QTEPIFEPSSSSSYEPLSCDTPQCNAL----EVSECRN 230

Query: 167 CNPKSRNCSGSCPGYGILYGSGA-TAGFLLSETLDFPKKGVPDFLVGCSVSS---VHQPA 226
                     +C  Y + YG G+ T G   +ETL      V +  VGC  S+       A
Sbjct: 231 ---------ATCL-YEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVGAA 290

Query: 227 GIAGFGRAPESLPSQMRLKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPF 286
           G+ G G    +LPSQ+    FSYCLV R  D     S   +D G+    S +   + AP 
Sbjct: 291 GLLGLGGGLLALPSQLNTTSFSYCLVDRDSD-----SASTVDFGT----SLSPDAVVAPL 350

Query: 287 RENPSGSNAAFREYYYLSLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKP 346
             N          +YYL L  I +GG+ ++IP      D +G GG IIDSG+  T L   
Sbjct: 351 LRNHQ-----LDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTE 410

Query: 347 IFEAVAGELEKQLVKYPRTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPV 406
           I+ ++     K  +   +   V +      C+++S + +VE P +   F GG  L+LP  
Sbjct: 411 IYNSLRDSFVKGTLDLEKAAGVAM---FDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAK 470

Query: 407 NYLALVTDAGVVCLTMMTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRC 460
           NY+  V   G  CL            A    I G  QQQ   V +DLA + IGF   +C
Sbjct: 471 NYMIPVDSVGTFCLAFAPT-------ASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of Bhi03G001383 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-48
Identity = 155/488 (31.76%), Postives = 235/488 (48.16%), Query Frame = 0


Query: 23  SISTITLPLTAFPSIPL-----TDDPLKIINYLLSASLNRAQHLKNPQTKSIQNVSLFSR 82
           S+S+++ PL    S  L     +  PL ++    S+S + A+  ++   +  Q +SL   
Sbjct: 21  SVSSLSTPLLLHLSHSLSTSKHSSSPLHLLK--SSSSRSSARFRRHHHKQQQQQLSLPIS 80

Query: 83  SYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFVP-KL 142
           S   Y I+L+ G+    +S   DTGS LVWFPC   + C  C     ++  +P   P  L
Sbjct: 81  SGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILC-----ESKPLPPSPPSSL 140

Query: 143 SSSAKIIGCRNPKCAWIFG--PNLN----SRCRNCNPKSRNCSGS---CPGYGILYGSGA 202
           SSSA  + C +P C+      P+ +    S C     ++ +C+ S   CP +   YG G+
Sbjct: 141 SSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGS 200

Query: 203 TAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRL------KRF 262
               L S++L  P   V +F  GC+ +++ +P G+AGFGR   SLP+Q+ +        F
Sbjct: 201 LVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSF 260

Query: 263 SYCLVSRGFDDSPV--SSPLVL--------------DSGSESDDSKTE--SFIYAPFREN 322
           SYCLVS  FD   V   SPL+L              D   + DD K +   F++    EN
Sbjct: 261 SYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLEN 320

Query: 323 PSGSNAAFREYYYLSLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFE 382
           P         +Y +SL+ I IG + +  P      D  G GG ++DSG+TFT L    + 
Sbjct: 321 PK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYN 380

Query: 383 AVAGELEKQLVK-YPRTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGG-AKLSLPPVN 442
           +V  E + ++ + + R   VE  SG+ PC+ +++ V V  P LVL F G  + ++LP  N
Sbjct: 381 SVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKV--PALVLHFAGNRSSVTLPRRN 440

Query: 443 YLALVTDAG--------VVCLTMMT--DVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNR 460
           Y     D G        + CL +M   D   + GG G   I G +QQQ   V YDL   R
Sbjct: 441 YFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTG--AILGNYQQQGFEVVYDLLNRR 491

[Close]

BLAST of Bhi03G001383 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.5e-35
Identity = 122/388 (31.44%), Postives = 177/388 (45.62%), Query Frame = 0


Query: 80  GAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFVPKLSSS 139
           G Y + +A GTP  + S + DTGS L+W  C     C+ C      +   P F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCF-----SQPTPIFNPQDSSS 153

Query: 140 AKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYGILYGSGATA-GFLLSETLD 199
              + C +  C               +  S  C+ +   Y   YG G+T  G++ +ET  
Sbjct: 154 FSTLPCESQYCQ--------------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 200 FPKKGVPDFLVGCSVSS----VHQPAGIAGFGRAPESLPSQMRLKRFSYCLVSRGFDDSP 259
           F    VP+   GC   +        AG+ G G  P SLPSQ+ + +FSYC+ S G   SP
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSSP 273

Query: 260 VSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLRRILIGGKPVKIPYK 319
            +  L   +    + S + + I++    NP+        YYY++L+ I +GG  + IP  
Sbjct: 274 STLALGSAASGVPEGSPSTTLIHSSL--NPT--------YYYITLQGITVGGDNLGIPSS 333

Query: 320 YLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTKSVEVQSGLRPCFDI 379
                  G GG IIDSG+T T+L +  + AVA     Q +  P     E  SGL  CF  
Sbjct: 334 TFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ-INLPTVD--ESSSGLSTCFQQ 393

Query: 380 SKEVS-VEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM--MTDVGVVGGGAGPAI 439
             + S V+ PE+ ++F GG  L+L   N L    + GV+CL M   + +G+         
Sbjct: 394 PSDGSTVQVPEISMQFDGGV-LNLGEQNILISPAE-GVICLAMGSSSQLGI--------S 435

Query: 440 IFGAFQQQNVLVEYDLARNRIGFRKQRC 460
           IFG  QQQ   V YDL    + F   +C
Sbjct: 454 IFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Bhi03G001383 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.3e-35
Identity = 118/387 (30.49%), Postives = 174/387 (44.96%), Query Frame = 0


Query: 80  GAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFVPKLSSS 139
           G Y + L+ GTP Q  S + DTGS L+W  C    +C N S         P F P+ SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQS--------TPIFNPQGSSS 152

Query: 140 AKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYGILYGSGA-TAGFLLSETLD 199
              + C +  C  +  P               CS +   Y   YG G+ T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 200 FPKKGVPDFLVGCSVSS----VHQPAGIAGFGRAPESLPSQMRLKRFSYCLVSRGFDDSP 259
           F    +P+   GC  ++        AG+ G GR P SLPSQ+ + +FSYC+   G   S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIG---SS 272

Query: 260 VSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLRRILIGGKPVKI-PY 319
             S L+L S + S  + + +       + P+        +YY++L  + +G   + I P 
Sbjct: 273 TPSNLLLGSLANSVTAGSPNTTLIQSSQIPT--------FYYITLNGLSVGSTRLPIDPS 332

Query: 320 KYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTKSVEVQSGLRPCFD 379
            + +  + G GG IIDSG+T T+     +++V  E   Q +  P        SG   CF 
Sbjct: 333 AFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNG--SSSGFDLCFQ 392

Query: 380 ISKEVS-VEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTDVGVVGGGAGPAII 439
              + S ++ P  V+ F GG  L LP  NY  +    G++CL M       G  +    I
Sbjct: 393 TPSDPSNLQIPTFVMHFDGG-DLELPSENYF-ISPSNGLICLAM-------GSSSQGMSI 434

Query: 440 FGAFQQQNVLVEYDLARNRIGFRKQRC 460
           FG  QQQN+LV YD   + + F   +C
Sbjct: 453 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Bhi03G001383 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.7e-35
Identity = 139/464 (29.96%), Postives = 190/464 (40.95%), Query Frame = 0


Query: 19  SSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQ---------------HLKN 78
           S S S S+ITL L    ++     P ++ +  L     R +               H   
Sbjct: 64  SDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPR 123

Query: 79  PQTKSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPN 138
           P   S   VS  S+  G Y   L  GTP + +  V DTGS +VW  C    RC + S P 
Sbjct: 124 PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPI 183

Query: 139 VDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYGILY 198
            D        P+ S +   I C +P C  +     N+R + C             Y + Y
Sbjct: 184 FD--------PRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCL------------YQVSY 243

Query: 199 GSGA-TAGFLLSETLDFPKKGVPDFLVGCSVSS---VHQPAGIAGFGRAPESLPSQMRLK 258
           G G+ T G   +ETL F +  V    +GC   +       AG+ G G+   S P Q   +
Sbjct: 244 GDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHR 303

Query: 259 ---RFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYY 318
              +FSYCLV R     P  S +V  + + S  ++     + P   NP         +YY
Sbjct: 304 FNQKFSYCLVDRSASSKP--SSVVFGNAAVSRIAR-----FTPLLSNPK-----LDTFYY 363

Query: 319 LSLRRILIGGKPVK-IPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVK 378
           + L  I +GG  V  +       D  G GG IIDSG++ T L +P + A+        V 
Sbjct: 364 VGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFR---VG 423

Query: 379 YPRTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLT 438
               K     S    CFD+S    V+ P +VL F+ GA +SLP  NYL  V   G  C  
Sbjct: 424 AKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFA 483

Query: 439 MMTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRC 460
                G +GG      I G  QQQ   V YDLA +R+GF    C
Sbjct: 484 F---AGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Bhi03G001383 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.0e-28
Identity = 116/397 (29.22%), Postives = 164/397 (41.31%), Query Frame = 0


Query: 72  VSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPK 131
           VS   +  G Y + +  G+PP++   V D+GS +VW  C     C  C +   D    P 
Sbjct: 121 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQP---CKLC-YKQSD----PV 180

Query: 132 FVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYGILYGSGA-TAG 191
           F P  S S   + C +  C  I     NS C          SG C  Y ++YG G+ T G
Sbjct: 181 FDPAKSGSYTGVSCGSSVCDRI----ENSGCH---------SGGC-RYEVMYGDGSYTKG 240

Query: 192 FLLSETLDFPKKGVPDFLVGCSVSSVHQ---PAGIAGFGRAPESLPSQMRLK---RFSYC 251
            L  ETL F K  V +  +GC   +       AG+ G G    S   Q+  +    F YC
Sbjct: 241 TLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYC 300

Query: 252 LVSRGFDD--SPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLRRI 311
           LVSRG D   S V     L  G+           + P   NP   +     +YY+ L+ +
Sbjct: 301 LVSRGTDSTGSLVFGREALPVGAS----------WVPLVRNPRAPS-----FYYVGLKGL 360

Query: 312 LIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTKSV 371
            +GG  + +P         G GG ++D+G+  T L    + A     + Q    PR   V
Sbjct: 361 GVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGV 420

Query: 372 EVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTDVGV 431
            +      C+D+S  VSV  P +   F  G  L+LP  N+L  V D+G  C         
Sbjct: 421 SI---FDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAF------ 470

Query: 432 VGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRC 460
                G +II G  QQ+ + V +D A   +GF    C
Sbjct: 481 AASPTGLSII-GNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Bhi03G001383 vs. NCBI nr
Match: XP_038881211.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 922.2 bits (2382), Expect = 1.8e-264
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH
Sbjct: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60

Query: 61  LKNPQTKSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCS 120
           LKNPQTKSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCS
Sbjct: 61  LKNPQTKSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCS 120

Query: 121 FPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYG 180
           FPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYG
Sbjct: 121 FPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYG 180

Query: 181 ILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRLKR 240
           ILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRLKR
Sbjct: 181 ILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRLKR 240

Query: 241 FSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLR 300
           FSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLR
Sbjct: 241 FSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLR 300

Query: 301 RILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTK 360
           RILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTK
Sbjct: 301 RILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTK 360

Query: 361 SVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTDV 420
           SVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTDV
Sbjct: 361 SVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTDV 420

Query: 421 GVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           GVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT
Sbjct: 421 GVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 460

[Close]

BLAST of Bhi03G001383 vs. NCBI nr
Match: XP_008462617.1 (PREDICTED: aspartic proteinase nepenthesin-2-like [Cucumis melo] >KAA0025220.1 aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 770.0 bits (1987), Expect = 1.2e-218
Identity = 385/464 (82.97%), Postives = 414/464 (89.22%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSSS   ITLPL  FPSIP T DPLK IN+LLSASL+RAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLATFPSIPFT-DPLKTINHLLSASLSRAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK+PQ+K   S +NVSLF RSYGAY+++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRC+
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
           +CSFP+VD ATI KFVPKLSSS KI+GCRNPKCAWIFGPNL SRCRNCNPKSR CS SCP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYGI YGSGATAG LLSETLD   K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CL+ RGFDDSPVSSPLVLDSG ESD+SKT+SFIYAPF+ENPS SN AFREYYYL
Sbjct: 241 LKRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS GKGGAIIDSGSTFTFLDKPIFEA+AGELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420
           R K +E ++GLRPCF+ISK E S EFPE+ LKFKGG KLSLPP NYL +VTDA VVCLTM
Sbjct: 361 RAKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTM 420

Query: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           MT+  VVG G GPAIIFGAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Bhi03G001383 vs. NCBI nr
Match: TYK07446.1 (aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 768.8 bits (1984), Expect = 2.6e-218
Identity = 385/464 (82.97%), Postives = 414/464 (89.22%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSSS   ITLPLT FPSIP T DPLK IN+LLSASL+RAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLTTFPSIPFT-DPLKTINHLLSASLSRAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK+PQ+K   S +NVSLF RSYGAY+++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRC+
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
           +CSFP+VD ATI KFVPKLSSS KI+GCRNPKCAWIFGPNL SRCRNCNPKSR CS SCP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYGI YGSGATAG LLSETLD   K VPDFLVGCSV SV QPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVRQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CL+ RGFDDSPVSSPLVLDSG ESD+SKT+SFIYAPF+ENPS SN AFREYYYL
Sbjct: 241 LKRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS GKGGAIIDSGSTFTFLDKPIFEA+AGELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420
           R K +E ++GLRPCF+ISK E S EFPE+ LKFKGG KLSLPP NYL +VTDA VVCLTM
Sbjct: 361 RAKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTM 420

Query: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           MT+  VVG G GPAIIFGAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Bhi03G001383 vs. NCBI nr
Match: XP_011657732.1 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN48299.1 hypothetical protein Csa_004059 [Cucumis sativus])

HSP 1 Score: 768.8 bits (1984), Expect = 2.6e-218
Identity = 389/464 (83.84%), Postives = 408/464 (87.93%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSS  ST  LPLT FPS+  T DP K IN LLSASLNRAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSS--STTVLPLTTFPSVSFT-DPFKTINLLLSASLNRAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK PQ+K   SIQNVSLF RSYGAYS++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRCS
Sbjct: 61  LKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
            CSFP VD ATI KFVPKLSSS K++GCRNPKCAWIFGPNL SRCRNCN KSR CS SCP
Sbjct: 121 RCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYG+ YGSGATAG LLSETLD   K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CLVSRGFDDSPVSSPLVLDSGSESD+SKT+SFIYAPFRENPS SNAAFREYYYL
Sbjct: 241 LKRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS G GGAIIDSGSTFTFLDKPIFEA+A ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420
           R K VE QSGLRPCF+I K E S EFP++VLKFKGG KLSL   NYLA+VTD GVVCLTM
Sbjct: 361 RAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTM 420

Query: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           MTD  VVGGG GPAII GAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

[Close]

BLAST of Bhi03G001383 vs. NCBI nr
Match: XP_022979057.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 765.8 bits (1976), Expect = 2.2e-217
Identity = 385/463 (83.15%), Postives = 412/463 (88.98%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEFFPI FL SI LLL  SSSSS  T+TLPLTAFPS+PLT  P K I +L+SASL RAQH
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLT-HPWKNIKHLVSASLARAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK P+TK   SIQNV+LF RSYGAYSI+LAFGTPPQ+LS VFDTGSSLVWFPCTAGYRCS
Sbjct: 61  LKTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
           NCSFPNVDAATIPKF+PKLSSSA+IIGCRN KC+WIFGPNL S CR+C+P+SR CS +CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYGI YGSGATAGFLLSETLDFP+K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CLV R FDDSPVSSPLVLDS  ES DSKT S IYAPFRENPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           +LRRILIG KPVK PYKYL+P+SAG GGAIIDSGSTFTFLDKPIFEAVA ELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMM 420
           R K VE +SGLRPCFDISKE SVEFPEL+LKFKGGA L+LPP NYLALVTD GVVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 TDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           TDV  +GGG GPAIIFGAFQQQNVLV+YDLA+ RIGFRKQRCT
Sbjct: 421 TDVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Bhi03G001383 vs. ExPASy TrEMBL
Match: A0A5A7SGF9 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00670 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.6e-219
Identity = 385/464 (82.97%), Postives = 414/464 (89.22%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSSS   ITLPL  FPSIP T DPLK IN+LLSASL+RAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLATFPSIPFT-DPLKTINHLLSASLSRAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK+PQ+K   S +NVSLF RSYGAY+++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRC+
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
           +CSFP+VD ATI KFVPKLSSS KI+GCRNPKCAWIFGPNL SRCRNCNPKSR CS SCP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYGI YGSGATAG LLSETLD   K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CL+ RGFDDSPVSSPLVLDSG ESD+SKT+SFIYAPF+ENPS SN AFREYYYL
Sbjct: 241 LKRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS GKGGAIIDSGSTFTFLDKPIFEA+AGELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420
           R K +E ++GLRPCF+ISK E S EFPE+ LKFKGG KLSLPP NYL +VTDA VVCLTM
Sbjct: 361 RAKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTM 420

Query: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           MT+  VVG G GPAIIFGAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Bhi03G001383 vs. ExPASy TrEMBL
Match: A0A1S3CHV2 (aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.6e-219
Identity = 385/464 (82.97%), Postives = 414/464 (89.22%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSSS   ITLPL  FPSIP T DPLK IN+LLSASL+RAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLATFPSIPFT-DPLKTINHLLSASLSRAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK+PQ+K   S +NVSLF RSYGAY+++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRC+
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
           +CSFP+VD ATI KFVPKLSSS KI+GCRNPKCAWIFGPNL SRCRNCNPKSR CS SCP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYGI YGSGATAG LLSETLD   K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CL+ RGFDDSPVSSPLVLDSG ESD+SKT+SFIYAPF+ENPS SN AFREYYYL
Sbjct: 241 LKRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS GKGGAIIDSGSTFTFLDKPIFEA+AGELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420
           R K +E ++GLRPCF+ISK E S EFPE+ LKFKGG KLSLPP NYL +VTDA VVCLTM
Sbjct: 361 RAKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTM 420

Query: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           MT+  VVG G GPAIIFGAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Bhi03G001383 vs. ExPASy TrEMBL
Match: A0A5D3CAS4 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001760 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 385/464 (82.97%), Postives = 414/464 (89.22%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSSS   ITLPLT FPSIP T DPLK IN+LLSASL+RAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLTTFPSIPFT-DPLKTINHLLSASLSRAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK+PQ+K   S +NVSLF RSYGAY+++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRC+
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
           +CSFP+VD ATI KFVPKLSSS KI+GCRNPKCAWIFGPNL SRCRNCNPKSR CS SCP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYGI YGSGATAG LLSETLD   K VPDFLVGCSV SV QPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVRQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CL+ RGFDDSPVSSPLVLDSG ESD+SKT+SFIYAPF+ENPS SN AFREYYYL
Sbjct: 241 LKRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS GKGGAIIDSGSTFTFLDKPIFEA+AGELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420
           R K +E ++GLRPCF+ISK E S EFPE+ LKFKGG KLSLPP NYL +VTDA VVCLTM
Sbjct: 361 RAKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTM 420

Query: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           MT+  VVG G GPAIIFGAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Bhi03G001383 vs. ExPASy TrEMBL
Match: A0A0A0KHK2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 389/464 (83.84%), Postives = 408/464 (87.93%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEF PIPFLFSIFLLLPTSSSS  ST  LPLT FPS+  T DP K IN LLSASLNRAQH
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSS--STTVLPLTTFPSVSFT-DPFKTINLLLSASLNRAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK PQ+K   SIQNVSLF RSYGAYS++LAFGTPPQNLSF+FDTGSSLVWFPCTAGYRCS
Sbjct: 61  LKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
            CSFP VD ATI KFVPKLSSS K++GCRNPKCAWIFGPNL SRCRNCN KSR CS SCP
Sbjct: 121 RCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYG+ YGSGATAG LLSETLD   K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQMR
Sbjct: 181 GYGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMR 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CLVSRGFDDSPVSSPLVLDSGSESD+SKT+SFIYAPFRENPS SNAAFREYYYL
Sbjct: 241 LKRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDS G GGAIIDSGSTFTFLDKPIFEA+A ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISK-EVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTM 420
           R K VE QSGLRPCF+I K E S EFP++VLKFKGG KLSL   NYLA+VTD GVVCLTM
Sbjct: 361 RAKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTM 420

Query: 421 MTDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           MTD  VVGGG GPAII GAFQQQNVLVEYDLA+ RIGFRKQ+CT
Sbjct: 421 MTDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

[Close]

BLAST of Bhi03G001383 vs. ExPASy TrEMBL
Match: A0A6J1IMR7 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.1e-217
Identity = 385/463 (83.15%), Postives = 412/463 (88.98%), Query Frame = 0


Query: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60
           MEFFPI FL SI LLL  SSSSS  T+TLPLTAFPS+PLT  P K I +L+SASL RAQH
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLT-HPWKNIKHLVSASLARAQH 60

Query: 61  LKNPQTK---SIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120
           LK P+TK   SIQNV+LF RSYGAYSI+LAFGTPPQ+LS VFDTGSSLVWFPCTAGYRCS
Sbjct: 61  LKTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180
           NCSFPNVDAATIPKF+PKLSSSA+IIGCRN KC+WIFGPNL S CR+C+P+SR CS +CP
Sbjct: 121 NCSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCP 180

Query: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240
           GYGI YGSGATAGFLLSETLDFP+K VPDFLVGCSV SVHQPAGIAGFGR PESLPSQM 
Sbjct: 181 GYGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMG 240

Query: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300
           LKRFS+CLV R FDDSPVSSPLVLDS  ES DSKT S IYAPFRENPSGSNAAFREYYYL
Sbjct: 241 LKRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360
           +LRRILIG KPVK PYKYL+P+SAG GGAIIDSGSTFTFLDKPIFEAVA ELEKQLVKYP
Sbjct: 301 TLRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360

Query: 361 RTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMM 420
           R K VE +SGLRPCFDISKE SVEFPEL+LKFKGGA L+LPP NYLALVTD GVVCLTM+
Sbjct: 361 RAKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMI 420

Query: 421 TDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 461
           TDV  +GGG GPAIIFGAFQQQNVLV+YDLA+ RIGFRKQRCT
Sbjct: 421 TDVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

The following BLAST results are available for this feature:

BLAST of Bhi03G001383 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	3.2e-134	52.65	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	1.4e-49	31.76	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	9.2e-49	31.85	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	1.1e-38	30.65	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	4.3e-38	30.07	Eukaryotic aspartyl protease family protein	[more]

BLAST of Bhi03G001383 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	2.0e-48	31.76	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q766C2	1.5e-35	31.44	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	4.3e-35	30.49	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LNJ3	9.7e-35	29.96	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	1.0e-28	29.22	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of Bhi03G001383 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881211.1	1.8e-264	100.00	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_008462617.1	1.2e-218	82.97	PREDICTED: aspartic proteinase nepenthesin-2-like [Cucumis melo] >KAA0025220.1 a...	[more]
TYK07446.1	2.6e-218	82.97	aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa]	[more]
XP_011657732.1	2.6e-218	83.84	probable aspartyl protease At4g16563 [Cucumis sativus] >KGN48299.1 hypothetical ...	[more]
XP_022979057.1	2.2e-217	83.15	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]

BLAST of Bhi03G001383 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SGF9	5.6e-219	82.97	Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CHV2	5.6e-219	82.97	aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 P...	[more]
A0A5D3CAS4	1.2e-218	82.97	Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KHK2	1.2e-218	83.84	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G45447...	[more]
A0A6J1IMR7	1.1e-217	83.15	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 88..108 score: 51.74 coord: 326..337 score: 36.31
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 265..460 e-value: 5.7E-49 score: 168.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 57..264 e-value: 5.5E-32 score: 113.2
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 77..459
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 295..455 e-value: 4.6E-35 score: 120.8
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 82..249 e-value: 3.9E-28 score: 98.8
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 8..459
None	No IPR available	PANTHER	PTHR47967:SF36	BNACNNG47670D PROTEIN	coord: 8..459
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 82..455 score: 32.918289
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 81..459 e-value: 5.29484E-77 score: 239.472

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M001383	Bhi03M001383	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity