Bhi03G001291 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G001291
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Proteinase inhibitor I25, cystatin
Location	chr3: 35062111 .. 35062524 (-)
RNA-Seq Expression	Bhi03G001291
Synteny	Bhi03G001291

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGTTTCTACAAAATATCAATCAAGGGTATTATATAATGAGTGAGATACCACCAAGTATTTGCCTTTGGTTTCCGATTAAGGATATCACTGATCCACATGTGCAAGAAATAGGAAAACTTCTGGTGGATGAGATTAACGAGGATAATACAAGGGAAAATCTGAAGTTCAAGCGTGTGGTGAATGGTCTGCGTTCAAACGAAGGCACATTATATAACCTTGTGTTGGAAGTTGAAACGTCCCAACAAATTAATTGGACATATGCGACCATCGTGTTAGAGGTTATCCAATCCGATGTACTAAAGCTTCGTATCATATCTTTTAAACCTGTTCTCGAGTACAAGATCTAATAATGAACTGTGCTCTCTACTTTACAAATTACAATATGCTTAAATAAGTTGTAACATTAACGTC

mRNA sequence

GAAAGTTTCTACAAAATATCAATCAAGGGTATTATATAATGAGTGAGATACCACCAAGTATTTGCCTTTGGTTTCCGATTAAGGATATCACTGATCCACATGTGCAAGAAATAGGAAAACTTCTGGTGGATGAGATTAACGAGGATAATACAAGGGAAAATCTGAAGTTCAAGCGTGTGGTGAATGGTCTGCGTTCAAACGAAGGCACATTATATAACCTTGTGTTGGAAGTTGAAACGTCCCAACAAATTAATTGGACATATGCGACCATCGTGTTAGAGGTTATCCAATCCGATGTACTAAAGCTTCGTATCATATCTTTTAAACCTGTTCTCGAGTACAAGATCTAATAATGAACTGTGCTCTCTACTTTACAAATTACAATATGCTTAAATAAGTTGTAACATTAACGTC

Coding sequence (CDS)

AAGTTTCTACAAAATATCAATCAAGGGTATTATATAATGAGTGAGATACCACCAAGTATTTGCCTTTGGTTTCCGATTAAGGATATCACTGATCCACATGTGCAAGAAATAGGAAAACTTCTGGTGGATGAGATTAACGAGGATAATACAAGGGAAAATCTGAAGTTCAAGCGTGTGGTGAATGGTCTGCGTTCAAACGAAGGCACATTATATAACCTTGTGTTGGAAGTTGAAACGTCCCAACAAATTAATTGGACATATGCGACCATCGTGTTAGAGGTTATCCAATCCGATGTACTAAAGCTTCGTATCATATCTTTTAAACCTGTTCTCGAGTACAAGATCTAA

Protein sequence

KFLQNINQGYYIMSEIPPSICLWFPIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSNEGTLYNLVLEVETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEYKI

Homology

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BLAST of Bhi03G001291 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 8.4e-04
Identity = 31/87 (35.63%), Postives = 45/87 (51.72%), Query Frame = 0


Query: 23  WFPIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRS-NEGTLYNLVLEVET-S 82
           W PIK+I DPH+QE+G+  + E N  +  + L F RV  G +    G  Y L +E  +  
Sbjct: 23  WSPIKNIDDPHIQELGRWAITENNRVSPSDELTFHRVTGGEQQVVSGMNYRLEIEAASGG 82

Query: 83  QQINWTYATIVLEVIQSDVLKLRIISF 108
             +  +Y  +V E   S+  KL  ISF
Sbjct: 83  GDVTGSYGAVVFEQEWSNTRKL--ISF 107

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_012607 [Cucumis sativus])

HSP 1 Score: 83.2 bits (204), Expect = 1.6e-12
Identity = 47/97 (48.45%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PI DI DPHVQ +G+L V+ +   N+ E LKF RVVNGL+S+        E  LY+LVLE
Sbjct: 12  PIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
            +T+++INWTYAT  LE +   +++   +SF+PVL Y
Sbjct: 72  AKTNEEINWTYAT-KLEEVSGGLIRHVFLSFEPVLPY 107

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 70.5 bits (171), Expect = 1.1e-08
Identity = 48/102 (47.06%), Postives = 61/102 (59.80%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PIKDI DP VQEIG L VD+ N+  T ENLKF RVVNGL +          GT+Y LVLE
Sbjct: 9   PIKDINDPQVQEIGNLTVDDHNK-KTGENLKFVRVVNGLFAGFVDPFSPLGGTVYELVLE 68

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRII----SFKPVLEYK 115
              + +INWTY   V     + +  L ++    SF+PVL+Y+
Sbjct: 69  AINAGKINWTYKVKV--SASTTIRPLGVVYNFQSFEPVLQYE 107

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 63.5 bits (153), Expect = 1.3e-06
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 25  PIKDITDP-HVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVL 84
           PI+DI D   VQ++G L VD+ N+  T ENLKF RVVNGL ++         G LY LVL
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNK-KTGENLKFVRVVNGLAASFSDPISPLGGVLYELVL 68

Query: 85  EVETSQQINWTYATIVLE---VIQSDVLKLRIISFKPVLEYK 115
           E  T+ +INWTY   V +   +     +     SFKPVLEY+
Sbjct: 69  EAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: DAD37832.1 (TPA_asm: hypothetical protein HUJ06_008473 [Nelumbo nucifera])

HSP 1 Score: 55.5 bits (132), Expect = 3.6e-04
Identity = 38/95 (40.00%), Postives = 51/95 (53.68%), Query Frame = 0


Query: 17  PPSICLWFPIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRS-NEGTLYNLVL 76
           PP +  W PI+ + DPHVQEI K  + E NE+  + NLKFKRV+ G      G  Y L++
Sbjct: 30  PPLLGGWQPIEHVKDPHVQEIAKFAITEHNEE-AKTNLKFKRVIRGEEQVVAGMNYRLIV 89

Query: 77  EVETSQQINWTYATIVLEVIQSDVLKLRIISFKPV 111
             +  +  N  Y  +V E +     KL   SFKPV
Sbjct: 90  AAKDGKVTN-NYEVVVWEKLWESFRKL--TSFKPV 120

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: TYK05428.1 (Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 54.7 bits (130), Expect = 6.2e-04
Identity = 38/81 (46.91%), Postives = 52/81 (64.20%), Query Frame = 0


Query: 25 PIKDITDP-HVQE-IGKLLVDEINEDNTRENLKFKRVVNGLRS---------NEGTLYNL 84
          PI++I D   VQ  IGKL V++ N+  T ENL+F  VVNGLRS         +EG LY+L
Sbjct: 12 PIENINDDLQVQSIIGKLAVEDHNK-KTGENLEFVDVVNGLRSEVFVQPGCTHEGILYHL 71

Query: 85 VLEVETSQQINWTYATIVLEV 95
          ++E +T + INWTY   +LE+
Sbjct: 72 LVEAKTIEGINWTYVAKLLEL 91

[Close]

BLAST of Bhi03G001291 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.6e-13
Identity = 48/97 (49.48%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PI DI DPHVQ IGKL V++ +  N  E LKF RVVNGL+S+        E  LY+LVLE
Sbjct: 12  PIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
            +T+++INWTYAT + +V    +++   +SF+PVL Y
Sbjct: 72  AKTNEEINWTYATKLEKVYSGCLIRHVFLSFEPVLPY 108

[Close]

BLAST of Bhi03G001291 vs. ExPASy TrEMBL
Match: A0A0A0KZ98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 7.9e-13
Identity = 47/97 (48.45%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PI DI DPHVQ +G+L V+ +   N+ E LKF RVVNGL+S+        E  LY+LVLE
Sbjct: 12  PIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
            +T+++INWTYAT  LE +   +++   +SF+PVL Y
Sbjct: 72  AKTNEEINWTYAT-KLEEVSGGLIRHVFLSFEPVLPY 107

[Close]

BLAST of Bhi03G001291 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-07
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 25  PIKDITDP-HVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVL 84
           PI+DI D   VQ++G L VD+ N+  T ENLKF RVVNGL ++         G LY LVL
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNK-KTGENLKFVRVVNGLAASFSDPISPLGGVLYELVL 68

Query: 85  EVETSQQINWTYATIVLE---VIQSDVLKLRIISFKPVLEYK 115
           E  T+ +INWTY   V +   +     +     SFKPVLEY+
Sbjct: 69  EAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

[Close]

BLAST of Bhi03G001291 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-07
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 25  PIKDITDP-HVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVL 84
           PI+DI D   VQ++G L VD+ N+  T ENLKF RVVNGL ++         G LY LVL
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNK-KTGENLKFVRVVNGLAASFSDPISPLGGVLYELVL 68

Query: 85  EVETSQQINWTYATIVLE---VIQSDVLKLRIISFKPVLEYK 115
           E  T+ +INWTY   V +   +     +     SFKPVLEY+
Sbjct: 69  EAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

[Close]

BLAST of Bhi03G001291 vs. ExPASy TrEMBL
Match: A0A0A0L3W1 (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.2e-05
Identity = 46/118 (38.98%), Postives = 68/118 (57.63%), Query Frame = 0


Query: 7   NQGYYIMSEIPPSICLWFPIKDITDP-HVQE-IGKLLVDEINEDNTRENLKFKRVVNGLR 66
           +Q Y IMS +  +     PI+DI D   VQ  IG+L V++ N+  T +NL+   VVNGLR
Sbjct: 4   HQRYCIMS-VEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNK-KTGDNLELVDVVNGLR 63

Query: 67  S---------NEGTLYNLVLEVETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
           S         +EG LY+L++E +T + INWTY   +LE+     ++    SF+P+L Y
Sbjct: 64  SGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPLLPY 119

The following BLAST results are available for this feature:

BLAST of Bhi03G001291 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi03G001291 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10Q46	8.4e-04	35.63	Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]

BLAST of Bhi03G001291 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN54823.1	1.6e-12	48.45	hypothetical protein Csa_012607 [Cucumis sativus]	[more]
KAE8649716.1	1.1e-08	47.06	hypothetical protein Csa_012476 [Cucumis sativus]	[more]
XP_008442277.1	1.3e-06	45.10	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys...	[more]
DAD37832.1	3.6e-04	40.00	TPA_asm: hypothetical protein HUJ06_008473 [Nelumbo nucifera]	[more]
TYK05428.1	6.2e-04	46.91	Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa]	[more]

BLAST of Bhi03G001291 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L191	4.6e-13	49.48	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE...	[more]
A0A0A0KZ98	7.9e-13	48.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1	[more]
A0A5A7TJK0	6.4e-07	45.10	Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5B9	6.4e-07	45.10	cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=...	[more]
A0A0A0L3W1	1.2e-05	38.98	Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 26..109 e-value: 1.1E-12 score: 48.1
None	No IPR available	GENE3D	3.10.450.10		coord: 23..114 e-value: 3.2E-12 score: 48.3
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 23..111
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 23..110
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 23..111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M001291	Bhi03M001291	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity