Bhi03G001108 (gene) Wax gourd (B227) v1

Overview
NameBhi03G001108
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptioniron-sulfur assembly protein IscA, chloroplastic-like
Locationchr3: 30189789 .. 30192896 (-)
RNA-Seq ExpressionBhi03G001108
SyntenyBhi03G001108
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACAAAAAAAAAGGATGAATAAAATATCTGAATCACTCGATCTCAACGCAAATAATATATCTTGACCGCACGATCCCAATTCACAAACACTACCGACTGGTCAAAGACGAGGTTCGCATATTCACCGGTCGCCGTTCACCGTTCGCCGGACTAATCGCGGCAGCATAACCGGATTTCTCCGATGGCGTTCTCTTCGATTACCATGAAATGCACCCCTATCCTTCACCTGCGAGCCCTTCCTCAAAGTTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCTCCTACGAAATTCAGCCGGAGGAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGGTACGCTCTCTTTTTTTATTTTTCCTTCTGTATTCCTCCTTTCTCTTTGGTTATTATACTTGACTATATCACCGTAGTTCATCGTCGTATTGTTTGCGTGAAATTAACCAGGACTTTTTTCCCCTTTGGATTGGATTGGAACTGTGAATTTGTGAAGAACGTCAGGGTTCGAGTTATTTTTCTAATTATTTAACGTAGGATCGATTTAGTGTTTGCTTGGAATCGAAATTGTTAGCTGGTGGAAGTCTCTTATTGAAATTAAGTAGCAGAATTTTGGGGGTTTTCTTGTTGAGTGACATGGAAAATAAATTTCCCCGCAAACTTGAACTAATGAGTTGTTTGTGAGTTTGGTGATATACAAACCATGAGTTTGCATCTTTCTTTTGTACTAATGCTCAAATGATTAGGATGGACTGATCTTCATGTGGGGGAAAAGATGACCATTATTTCTCGTGTTCGATAGCATTGATAATTCATTCTTTTTATGTGTGTTTGGGAGTGACTTTGAAATTGTTGAAATCATTTTTGTCATAATCAAAATCACTCCAAAATATATCTTTTACATTAAAAATCAATTTAGTTTTTCATTTTTAAATGTTATTTTCATATCACCAAATTTTGATTCAAAAGCATGTATTAAGTGATTTTGAACATGAAAAAATGATTTTAACTCTTTCAAAAAAATAACTCCAGACATGCCCTTAGACATATTCAGGTGTAATATTATTTGTTTTATGACACTCTCCCTTTGCGGCCTACGGCAGAACGAAATATAAAACCTACTGAATCTTGTATTTCAGTTTTTGCATATGAAATATGATGAGTATATCATGTTTTATTATCAAAGTTTGCCTTTTCATCTTATGTTTTTAATATGTAAATTGTGATTAAACAGCTGCGCCAGCAACCGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTATGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACTATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGTTTTGTGATAGGTAAGAGTTACAGTTTTTGCTTTTTTCACTATGAATTTCTAGTGAGGTTTTTAGTAATAGTGCAAATGTTTGAAGAAATCCTTTCTGAATGCATTATACTTGTAGATGATCATTTGCTTTATTTATACACACACTTTTGCGTGGGTGGGGGGTAATTTGTCAATGAGCTCCAAAACAGTGGAATATCTAAGTCTAGTTTTGCATATTTTTGGATTGGTAGTGTGGAATGGTAGAGGGCAAGTTCTCAATTTTTCTTAGGATAAAAGAAGCTTACTCTTTGTACATACTCTACAAGAATAATTTTACTCTATAGTTCATAATCTCTCTTAGTTCAAAAAGAAAACAGTGAAGTGTCTAAGGGTGTGTTAGAATACATTTTCAAATGTTTAATTTAAAAAATAAGTCATTTTGGAAGAAATTAGAGTGTTTGGCAGCCACTCAAAATAGTTTTTCAAGTGAATTTTAATCGGTGTTTATCAAAAGTGTTTAAATAAAGTATGTTTTTTGAAAAACTCTTTTTCCTCAAGTCAATCCAAACGGGCACTAAATCTAGTTTGCATACTTAGTTTGCATACTTTTGGATACGTTCTTTCCCTTGCAATGGTAGAGGGCAAGTTTTCAATGTTTCTTGGATGAAAATTACATGAACCACATGCCTTAAATTTGAAGGACTCTACCCAAATAATTTTACTCCAGAGTTCATAATCCATCACTTCTCTTGGAGACTCAAATCTCAATGACTTGAATTATTAGATACGAGCAATTTCTTATGCCAAGTGATAAGCAGGTTGCGCAACTACTTATGTCAAATATACTTGTCTTTAATGTATACTGTTCAACATAAACTTGTATGTTATTTCAATTCCTCGAAAATTGCCAAAATGAGCCTAGTTTAATTGTTATATAGAATGTGTTAGTAACCTAGAAGTATTTGGTTTGAATTTTCACGTTCATTGTACTTTAAAAGAAGAAAAAAAAAATCATTGGTAACTGCTCTCTACTCTGTTGATTAAGAAAAGTAAGCATCATCTAGTGAACTAAGAAAGTATCGTTTTGTCATTTTTCCTCTATGGCCTTTCAAGTTGTAGCCTCGGAGACGAATTTGATTTCACTTTTAGCTTGAACTTTTGATACAAACTTCCCACTCTTCTTGTTTTGTAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGGAAATCATTTGCTGCAGAAGTGTAGGATCGTCATCTGTATATATGAATGATTCAATAGAAACTGGATAGTTTCAGGTTAAAGAAAACTTAGAATGTGTAAAAACTCTTAGTTTGCTTGACAATTGATCCTAGCATACCTTCTATTACAAGCCCTTTGTTCGTGATATTTCTGCTTTAGCCCTTCTGGAAAAAAGAATTAGGCTTTTAGCTCTTCCTGTTTACTTTCTTACAGATTCTGTATTTGTTGGATAAAATATTATAGAATTCATGTATTTATTAAATATAGGCTAAATTATATATATATATATGTATACTTTAGAACTTTTTTTAATGTTTAAAAAATATCCATAGTCTTCAAAAGTTATAATATTA

mRNA sequence

AAAAAAACAAAAAAAAAGGATGAATAAAATATCTGAATCACTCGATCTCAACGCAAATAATATATCTTGACCGCACGATCCCAATTCACAAACACTACCGACTGGTCAAAGACGAGGTTCGCATATTCACCGGTCGCCGTTCACCGTTCGCCGGACTAATCGCGGCAGCATAACCGGATTTCTCCGATGGCGTTCTCTTCGATTACCATGAAATGCACCCCTATCCTTCACCTGCGAGCCCTTCCTCAAAGTTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCTCCTACGAAATTCAGCCGGAGGAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCAACCGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTATGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACTATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGTTTTGTGATAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGGAAATCATTTGCTGCAGAAGTGTAGGATCGTCATCTGTATATATGAATGATTCAATAGAAACTGGATAGTTTCAGGTTAAAGAAAACTTAGAATGTGTAAAAACTCTTAGTTTGCTTGACAATTGATCCTAGCATACCTTCTATTACAAGCCCTTTGTTCGTGATATTTCTGCTTTAGCCCTTCTGGAAAAAAGAATTAGGCTTTTAGCTCTTCCTGTTTACTTTCTTACAGATTCTGTATTTGTTGGATAAAATATTATAGAATTCATGTATTTATTAAATATAGGCTAAATTATATATATATATATGTATACTTTAGAACTTTTTTTAATGTTTAAAAAATATCCATAGTCTTCAAAAGTTATAATATTA

Coding sequence (CDS)

ATGGCGTTCTCTTCGATTACCATGAAATGCACCCCTATCCTTCACCTGCGAGCCCTTCCTCAAAGTTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCTCCTACGAAATTCAGCCGGAGGAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCAACCGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTATGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACTATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGTTTTGTGATAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGGAAATCATTTGCTGCAGAAGTGTAG

Protein sequence

MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV
Homology
[Close]
BLAST of Bhi03G001108 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 242.3 bits (617), Expect = 2.9e-64
Identity = 119/149 (79.87%), Postives = 130/149 (87.25%), Query Frame = 0 

Query: 27  SSISF---RYSPTKFSRRKSLSIRSVSVPAAPATEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENRANARPDDS IEY GF IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           DALIGGGFSF NPNATQTCGCGKSFAAE+
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]
BLAST of Bhi03G001108 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 79.0 bits (193), Expect = 4.3e-15
Identity = 49/158 (31.01%), Postives = 78/158 (49.37%), Query Frame = 0 

Query: 17  RALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGIA-PAISLTDNALKHLN 76
           R+L +  AP  +   R +    +   + +I+  S  ++   E  +   + L+DN ++ + 
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 77  KMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFL 136
           +++S   E   LR+GV  GGCSG  Y  E +NR N  PDD + E NG  +V D  S   +
Sbjct: 63  ELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTN--PDDRVFEKNGVKLVVDNVSYDLV 122

Query: 137 FGMQLDYSDALIGGGFSFK-NPNATQTCGCGKSFAAEV 173
            G  +DY + LI   F    NP+A   C C  SF  ++
Sbjct: 123 KGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVKL 158

[Close]
BLAST of Bhi03G001108 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.5e-12
Identity = 34/105 (32.38%), Postives = 60/105 (57.14%), Query Frame = 0 

Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGF 123
           ++L+D A   + ++   + +   LR+ V   GC+G+SY + +         D ++E  G 
Sbjct: 6   LALSDTAAARIRQLLQHQQKPF-LRLAVEAKGCNGLSYVLNYAQEKG--KFDEVVEEKGV 65

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
            I+ DPK+++ + G ++D+ D  +   F F NPNAT+ CGCG+SF
Sbjct: 66  KILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNATK-CGCGESF 106

[Close]
BLAST of Bhi03G001108 vs. ExPASy Swiss-Prot
Match: Q9XIK3 (Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISCA PE=2 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 4.1e-63
Identity = 119/149 (79.87%), Postives = 130/149 (87.25%), Query Frame = 0 

Query: 27  SSISF---RYSPTKFSRRKSLSIRSVSVPAAPATEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENRANARPDDS IEY GF IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           DALIGGGFSF NPNATQTCGCGKSFAAE+
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]
BLAST of Bhi03G001108 vs. ExPASy Swiss-Prot
Match: P72731 (Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1417 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.5e-36
Identity = 70/113 (61.95%), Postives = 85/113 (75.22%), Query Frame = 0 

Query: 56  ATEGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDD 115
           AT   A  I L+D ALKHL  ++ ++ +DLCLR+GVRQGGCSGMSY M+FE    A   D
Sbjct: 4   ATATQAKGIQLSDAALKHLLALKEQQGKDLCLRVGVRQGGCSGMSYMMDFEEPNRATEHD 63

Query: 116 SIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
            + +Y GF I+CD KSLL+L+G+ LDYS+ALIGGGF F NPNA QTCGCGKSF
Sbjct: 64  EVFDYEGFQIICDRKSLLYLYGLMLDYSNALIGGGFQFTNPNANQTCGCGKSF 116

[Close]
BLAST of Bhi03G001108 vs. ExPASy Swiss-Prot
Match: P51217 (Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.9e-32
Identity = 59/106 (55.66%), Postives = 83/106 (78.30%), Query Frame = 0 

Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGF 123
           I+LT+ A + +  ++S  + ++CLRIGV+QGGCSGMSY+M FE+ ++ R  D ++  + F
Sbjct: 8   ITLTEPAARQIKILKSNFDNEVCLRIGVKQGGCSGMSYSMNFESVSSLRETDEVLMLDSF 67

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           ++ CDPKSLL+L+G+ LDYS  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 68  LVACDPKSLLYLYGLSLDYSSELIGGGFQFSNPNASQTCGCGKSFS 113

[Close]
BLAST of Bhi03G001108 vs. ExPASy Swiss-Prot
Match: Q1XDR9 (Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.6e-30
Identity = 61/112 (54.46%), Postives = 81/112 (72.32%), Query Frame = 0 

Query: 58  EGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSI 117
           EG    I++T  ALK +  ++++   D+ LRIGVRQGGCSGMSY+M FE+    +  D  
Sbjct: 2   EGNMGFITITKPALKQIAILKNDHENDVHLRIGVRQGGCSGMSYSMNFEHVDKLKDTDER 61

Query: 118 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           +  + F +VCDPKSLL+L+G+ LD+S  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 62  LRLDNFSVVCDPKSLLYLYGLSLDFSSELIGGGFQFSNPNASQTCGCGKSFS 113

[Close]
BLAST of Bhi03G001108 vs. ExPASy Swiss-Prot
Match: Q9MSA1 (Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.0e-30
Identity = 58/107 (54.21%), Postives = 81/107 (75.70%), Query Frame = 0 

Query: 64  ISLTDNALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNG 123
           I++T +AL HLN +    +ED L +RIG++QGGCSG+SY M +E ++N   +D + +Y+ 
Sbjct: 6   INITKSALDHLNSISKLNSEDKLYIRIGIKQGGCSGLSYFMTYEKKSNISDNDLVYDYDN 65

Query: 124 FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           F +VCD KS+L+L+G+ LDYS +LI GGF F NPNA QTCGCGKSF+
Sbjct: 66  FTLVCDNKSILYLYGISLDYSSSLIDGGFKFLNPNAKQTCGCGKSFS 112

[Close]
BLAST of Bhi03G001108 vs. NCBI nr
Match: XP_038880838.1 (iron-sulfur assembly protein IscA, chloroplastic [Benincasa hispida])

HSP 1 Score: 344.4 bits (882), Expect = 5.8e-91
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI
Sbjct: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 172

[Close]
BLAST of Bhi03G001108 vs. NCBI nr
Match: XP_022950286.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_023544833.1 iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6604246.1 Iron-sulfur assembly protein IscA, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 336.3 bits (861), Expect = 1.6e-88
Identity = 166/172 (96.51%), Postives = 171/172 (99.42%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSITMKC P+LHLRALP+SSAPQSSISFRYSPTKFSRRK+LSIRSVSVPAAPA+EGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAE+
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]
BLAST of Bhi03G001108 vs. NCBI nr
Match: KAG7034403.1 (Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.7 bits (857), Expect = 4.6e-88
Identity = 165/172 (95.93%), Postives = 171/172 (99.42%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSIT+KC P+LHLRALP+SSAPQSSISFRYSPTKFSRRK+LSIRSVSVPAAPA+EGI
Sbjct: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAE+
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]
BLAST of Bhi03G001108 vs. NCBI nr
Match: XP_022977954.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 331.3 bits (848), Expect = 5.1e-87
Identity = 164/172 (95.35%), Postives = 169/172 (98.26%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSITMKC P+LHLRALP+SSAPQSSISFRYSPTKFSRRK+LSIRSVSVPAAPA+EGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAE+
Sbjct: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]
BLAST of Bhi03G001108 vs. NCBI nr
Match: XP_008440129.1 (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo])

HSP 1 Score: 322.8 bits (826), Expect = 1.8e-84
Identity = 161/172 (93.60%), Postives = 167/172 (97.09%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSITMK  P+LHLRALP+SSAPQSSISFR+SPTKFSR KSLSIRSVSVPAAPA+E I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AE+
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]
BLAST of Bhi03G001108 vs. ExPASy TrEMBL
Match: A0A6J1GEF0 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111453419 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 7.7e-89
Identity = 166/172 (96.51%), Postives = 171/172 (99.42%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSITMKC P+LHLRALP+SSAPQSSISFRYSPTKFSRRK+LSIRSVSVPAAPA+EGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAE+
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]
BLAST of Bhi03G001108 vs. ExPASy TrEMBL
Match: A0A6J1ISS5 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478089 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 2.5e-87
Identity = 164/172 (95.35%), Postives = 169/172 (98.26%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSITMKC P+LHLRALP+SSAPQSSISFRYSPTKFSRRK+LSIRSVSVPAAPA+EGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAE+
Sbjct: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]
BLAST of Bhi03G001108 vs. ExPASy TrEMBL
Match: A0A1S3B105 (iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484688 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 8.8e-85
Identity = 161/172 (93.60%), Postives = 167/172 (97.09%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSSITMK  P+LHLRALP+SSAPQSSISFR+SPTKFSR KSLSIRSVSVPAAPA+E I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AE+
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]
BLAST of Bhi03G001108 vs. ExPASy TrEMBL
Match: A0A6J1BTR2 (iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005271 PE=3 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.4e-82
Identity = 157/172 (91.28%), Postives = 164/172 (95.35%), Query Frame = 0 

Query: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60
           MAFSS TMK   +LHLRALP+SSA QSSISFRYS TKFSRRKSLS+RSVSVPAAP +EGI
Sbjct: 1   MAFSSATMKFPSVLHLRALPESSATQSSISFRYSLTKFSRRKSLSVRSVSVPAAPTSEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNAL HLNKMRS+RNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALNHLNKMRSDRNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA++
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAADM 172

[Close]
BLAST of Bhi03G001108 vs. ExPASy TrEMBL
Match: A0A0A0KJ66 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950 PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 2.0e-81
Identity = 157/174 (90.23%), Postives = 165/174 (94.83%), Query Frame = 0 

Query: 1   MAFSSITMK--CTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATE 60
           MAFSSI+MK  C P+LHL ALP+S APQ+SISFR+  TKFSRRKSLSIRSVSVPAAPA+E
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 173
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AE+
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

The following BLAST results are available for this feature:
BLAST of Bhi03G001108 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G10500.12.9e-6479.87chloroplast-localized ISCA-like protein [more]
AT5G03905.14.3e-1531.01Iron-sulphur cluster biosynthesis family protein [more]
AT2G36260.11.5e-1232.38Iron-sulphur cluster biosynthesis family protein [more]
BLAST of Bhi03G001108 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9XIK34.1e-6379.87Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
P727311.5e-3661.95Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ... [more]
P512172.9e-3255.66Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1[more]
Q1XDR91.6e-3054.46Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1[more]
Q9MSA16.0e-3054.21Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 S... [more]
BLAST of Bhi03G001108 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038880838.15.8e-91100.00iron-sulfur assembly protein IscA, chloroplastic [Benincasa hispida][more]
XP_022950286.11.6e-8896.51iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_0... [more]
KAG7034403.14.6e-8895.93Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. ... [more]
XP_022977954.15.1e-8795.35iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima][more]
XP_008440129.11.8e-8493.60PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo][more]
BLAST of Bhi03G001108 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GEF07.7e-8996.51iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3... [more]
A0A6J1ISS52.5e-8795.35iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=366... [more]
A0A1S3B1058.8e-8593.60iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1BTR21.4e-8291.28iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charant... [more]
A0A0A0KJ662.0e-8190.23Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016092FeS cluster insertion proteinTIGRFAMTIGR00049TIGR00049coord: 64..169
e-value: 2.8E-37
score: 125.1
IPR000361FeS cluster biogenesisPFAMPF01521Fe-S_biosyncoord: 64..166
e-value: 4.5E-22
score: 78.3
IPR035903HesB-like domain superfamilyGENE3D2.60.300.12coord: 63..171
e-value: 1.4E-35
score: 123.7
IPR035903HesB-like domain superfamilySUPERFAMILY89360HesB-like domaincoord: 63..159
IPR031108FeS assembly protein IscA, plant/cyanobacteria typePANTHERPTHR47265IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTICcoord: 1..171
NoneNo IPR availablePANTHERPTHR47265:SF1IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTICcoord: 1..171
IPR017870FeS cluster insertion, C-terminal, conserved sitePROSITEPS01152HESBcoord: 151..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001108Bhi03M001108mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0030674 protein-macromolecule adaptor activity