Bhi03G001029 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G001029
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	cysteine proteinase inhibitor 5-like
Location	chr3: 27680846 .. 27681151 (+)
RNA-Seq Expression	Bhi03G001029
Synteny	Bhi03G001029

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTCTCAAGTGCCTGGCAGTGGTTATTATGGTCCATGTGAGGATCCAGAAGGTCCACAAGTGAAAGGAAAAGCAGAATGGACAGTAGCAATCTACAACGAAAAAGAGGGAACAACCTTAAAACTCATTTGTGTTGTGAAGTGTGAGTCGCAGGTTGTGGCCGGAGTCAAGTACCGCCTTGTCTTGTTGCTTAAGGATGAAAAAACTAATTGTGAGAATTATTTCTTGGTTGTTGTGTGGGATAAGGAACATCACAATTACAGGCTGCTCGAGCTCATTTCTTTTGAACCTCTCCTCATATAA

mRNA sequence

ATGTGTTCTCAAGTGCCTGGCAGTGGTTATTATGGTCCATGTGAGGATCCAGAAGGTCCACAAGTGAAAGGAAAAGCAGAATGGACAGTAGCAATCTACAACGAAAAAGAGGGAACAACCTTAAAACTCATTTGTGTTGTGAAGTGTGAGTCGCAGGTTGTGGCCGGAGTCAAGTACCGCCTTGTCTTGTTGCTTAAGGATGAAAAAACTAATTGTGAGAATTATTTCTTGGTTGTTGTGTGGGATAAGGAACATCACAATTACAGGCTGCTCGAGCTCATTTCTTTTGAACCTCTCCTCATATAA

Coding sequence (CDS)

ATGTGTTCTCAAGTGCCTGGCAGTGGTTATTATGGTCCATGTGAGGATCCAGAAGGTCCACAAGTGAAAGGAAAAGCAGAATGGACAGTAGCAATCTACAACGAAAAAGAGGGAACAACCTTAAAACTCATTTGTGTTGTGAAGTGTGAGTCGCAGGTTGTGGCCGGAGTCAAGTACCGCCTTGTCTTGTTGCTTAAGGATGAAAAAACTAATTGTGAGAATTATTTCTTGGTTGTTGTGTGGGATAAGGAACATCACAATTACAGGCTGCTCGAGCTCATTTCTTTTGAACCTCTCCTCATATAA

Protein sequence

MCSQVPGSGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKDEKTNCENYFLVVVWDKEHHNYRLLELISFEPLLI

Homology

[Close]

BLAST of Bhi03G001029 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 2.3e-08
Identity = 35/90 (38.89%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 9   GYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKDE 68
           G + P  +   PQV    E+ V+ YN++  + LK   VV  E+QVV+G  YRL +   D 
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDG 88

Query: 69  KTNCENYFLVVVWDKEHHNYRLLELISFEP 99
               +NY L +VWDK    +R   L SFEP
Sbjct: 89  DGVSKNY-LAIVWDKPWMKFR--NLTSFEP 115

[Close]

BLAST of Bhi03G001029 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 3.2e-07
Identity = 35/90 (38.89%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 9   GYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKDE 68
           G + P  +   PQV    E+ V+ YN++  + LK   VV  E+QVV+G  YRL +   D 
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDG 88

Query: 69  KTNCENYFLVVVWDKEHHNYRLLELISFEP 99
               +NY L +VWDK    +R   L SFEP
Sbjct: 89  DGVSKNY-LAIVWDKPWMKFR--NLTSFEP 115

[Close]

BLAST of Bhi03G001029 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 44.3 bits (103), Expect = 9.6e-04
Identity = 28/84 (33.33%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 8   SGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKD 67
           +G + P E+    +V+  A++ V+ +N++    L+   VV+  +QVVAG  YRLV+  KD
Sbjct: 29  AGGWRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKD 88

Query: 68  EKTNCENYFLVVVWDKEHHNYRLL 92
                   +  VVWDK   ++R L
Sbjct: 89  GAV--VGNYEAVVWDKPWMHFRNL 110

[Close]

BLAST of Bhi03G001029 vs. NCBI nr
Match: XP_008441065.1 (PREDICTED: cysteine proteinase inhibitor 5-like [Cucumis melo] >KAA0025519.1 cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 99.0 bits (245), Expect = 2.5e-17
Identity = 47/97 (48.45%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 5   VPGSGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLL 64
           VPG+G Y P +DP+ P +K  AEW VA YN+KEGT L+ +C+++CESQ+V GV YR +L 
Sbjct: 6   VPGAGGYTPVKDPQSPHMKELAEWAVAEYNKKEGTHLRFVCILECESQIVEGVNYRFILK 65

Query: 65  LKDEKTNCENYFLVVVWDKEHHNYRLLELISFEPLLI 102
             DEK N  NY   +VW+K   +++  EL+ F+ LL+
Sbjct: 66  ANDEKANEGNY-EAIVWEKTWEDFK--ELVYFKQLLL 99

[Close]

BLAST of Bhi03G001029 vs. NCBI nr
Match: KAE8647756.1 (hypothetical protein Csa_003034 [Cucumis sativus])

HSP 1 Score: 90.9 bits (224), Expect = 6.8e-15
Identity = 49/92 (53.26%), Postives = 62/92 (67.39%), Query Frame = 0


Query: 9   GYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKDE 68
           G Y PC+DP  P VK  AEW VA YN+ +G  L L+ ++KCESQVVAGV +RLVL  KDE
Sbjct: 220 GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 279

Query: 69  KTNCENYFLVVVWDKEHHNYRLLELISFEPLL 101
             N E  +  VVW+K   N+R  +LI+F+ LL
Sbjct: 280 -NNGEGNYETVVWEKIWENFR--QLITFDHLL 308

[Close]

BLAST of Bhi03G001029 vs. NCBI nr
Match: NP_001267677.1 (cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein proteinase inhibitor [Cucumis sativus])

HSP 1 Score: 90.9 bits (224), Expect = 6.8e-15
Identity = 49/92 (53.26%), Postives = 62/92 (67.39%), Query Frame = 0


Query: 9   GYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKDE 68
           G Y PC+DP  P VK  AEW VA YN+ +G  L L+ ++KCESQVVAGV +RLVL  KDE
Sbjct: 7   GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 66

Query: 69  KTNCENYFLVVVWDKEHHNYRLLELISFEPLL 101
             N E  +  VVW+K   N+R  +LI+F+ LL
Sbjct: 67  -NNGEGNYETVVWEKIWENFR--QLITFDHLL 95

[Close]

BLAST of Bhi03G001029 vs. NCBI nr
Match: KAE8647459.1 (hypothetical protein Csa_003259 [Cucumis sativus])

HSP 1 Score: 89.0 bits (219), Expect = 2.6e-14
Identity = 43/98 (43.88%), Postives = 65/98 (66.33%), Query Frame = 0


Query: 4   QVPGSGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVL 63
           + PG+G Y P +DP+  ++K  AEW V  YN+KEGT L+ +C++ CESQ+V GV YR +L
Sbjct: 5   RAPGAGGYTPVKDPQSLRMKELAEWAVEEYNKKEGTHLRFVCILVCESQIVEGVNYRFIL 64

Query: 64  LLKDEKTNCENYFLVVVWDKEHHNYRLLELISFEPLLI 102
            +KDE  N  NY   +VW+K   +++   L+ F+ LL+
Sbjct: 65  RVKDENDNEGNY-EAIVWEKTWEHFK--GLVYFKQLLL 99

[Close]

BLAST of Bhi03G001029 vs. NCBI nr
Match: KAE8648197.1 (hypothetical protein Csa_018520 [Cucumis sativus])

HSP 1 Score: 87.4 bits (215), Expect = 7.6e-14
Identity = 47/90 (52.22%), Postives = 59/90 (65.56%), Query Frame = 0


Query: 11  YGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKDEKT 70
           Y P +D + P +K  AEWTVA YN++ G  L L  VVKCESQ+VAGV YR +L  KDE  
Sbjct: 4   YTPVQDLDNPHIKELAEWTVAEYNKEGGYQLALSSVVKCESQIVAGVNYRFILTAKDEND 63

Query: 71  NCENYFLVVVWDKEHHNYRLLELISFEPLL 101
           N  NY   V+W+K    +R  +LISF+PLL
Sbjct: 64  NEGNY-EAVIWEKVWDQFR--KLISFQPLL 90

[Close]

BLAST of Bhi03G001029 vs. ExPASy TrEMBL
Match: A0A5A7SJM4 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G00050 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.2e-17
Identity = 47/97 (48.45%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 5   VPGSGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLL 64
           VPG+G Y P +DP+ P +K  AEW VA YN+KEGT L+ +C+++CESQ+V GV YR +L 
Sbjct: 6   VPGAGGYTPVKDPQSPHMKELAEWAVAEYNKKEGTHLRFVCILECESQIVEGVNYRFILK 65

Query: 65  LKDEKTNCENYFLVVVWDKEHHNYRLLELISFEPLLI 102
             DEK N  NY   +VW+K   +++  EL+ F+ LL+
Sbjct: 66  ANDEKANEGNY-EAIVWEKTWEDFK--ELVYFKQLLL 99

[Close]

BLAST of Bhi03G001029 vs. ExPASy TrEMBL
Match: A0A1S3B2L7 (cysteine proteinase inhibitor 5-like OS=Cucumis melo OX=3656 GN=LOC103485290 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.2e-17
Identity = 47/97 (48.45%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 5   VPGSGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLL 64
           VPG+G Y P +DP+ P +K  AEW VA YN+KEGT L+ +C+++CESQ+V GV YR +L 
Sbjct: 6   VPGAGGYTPVKDPQSPHMKELAEWAVAEYNKKEGTHLRFVCILECESQIVEGVNYRFILK 65

Query: 65  LKDEKTNCENYFLVVVWDKEHHNYRLLELISFEPLLI 102
             DEK N  NY   +VW+K   +++  EL+ F+ LL+
Sbjct: 66  ANDEKANEGNY-EAIVWEKTWEDFK--ELVYFKQLLL 99

[Close]

BLAST of Bhi03G001029 vs. ExPASy TrEMBL
Match: O80389 (Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.3e-15
Identity = 49/92 (53.26%), Postives = 62/92 (67.39%), Query Frame = 0


Query: 9   GYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLLKDE 68
           G Y PC+DP  P VK  AEW VA YN+ +G  L L+ ++KCESQVVAGV +RLVL  KDE
Sbjct: 7   GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 66

Query: 69  KTNCENYFLVVVWDKEHHNYRLLELISFEPLL 101
             N E  +  VVW+K   N+R  +LI+F+ LL
Sbjct: 67  -NNGEGNYETVVWEKIWENFR--QLITFDHLL 95

[Close]

BLAST of Bhi03G001029 vs. ExPASy TrEMBL
Match: A0A0A0KIY5 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483248 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.1e-14
Identity = 43/96 (44.79%), Postives = 64/96 (66.67%), Query Frame = 0


Query: 6   PGSGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYRLVLLL 65
           PG+G Y P +DP+  ++K  AEW V  YN+KEGT L+ +C++ CESQ+V GV YR +L +
Sbjct: 7   PGAGGYTPVKDPQSLRMKELAEWAVEEYNKKEGTHLRFVCILVCESQIVEGVNYRFILRV 66

Query: 66  KDEKTNCENYFLVVVWDKEHHNYRLLELISFEPLLI 102
           KDE  N  NY   +VW+K   +++   L+ F+ LL+
Sbjct: 67  KDENDNEGNY-EAIVWEKTWEHFK--GLVYFKQLLL 99

[Close]

BLAST of Bhi03G001029 vs. ExPASy TrEMBL
Match: A0A0A0KF46 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483245 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 4.8e-14
Identity = 48/103 (46.60%), Postives = 62/103 (60.19%), Query Frame = 0


Query: 1   MCSQVPGSGYYGPCEDPEGPQVKGKAEWTVAIYNEKEGTTLKLICVVKCESQVVAGVKYR 60
           M S  PG   Y P +DP+ P++K  AEW VA +N+KEGT L+ I ++KCESQ+V GV YR
Sbjct: 1   MASSEPGG--YTPVKDPQSPRMKELAEWAVAEHNKKEGTHLRFISILKCESQIVNGVNYR 60

Query: 61  LVLLLKDEKTNC--ENYFLVVVWDKEHHNYRLLELISFEPLLI 102
             L  KDE  NC   NY  +V      H   L EL+ F+ LL+
Sbjct: 61  FTLTAKDESDNCLPGNYMAIVYEQLSEH---LKELVFFKQLLL 98

The following BLAST results are available for this feature:

BLAST of Bhi03G001029 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	2.3e-08	38.89	Cystatin/monellin superfamily protein	[more]

BLAST of Bhi03G001029 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41916	3.2e-07	38.89	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
P86472	9.6e-04	33.33	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]

BLAST of Bhi03G001029 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008441065.1	2.5e-17	48.45	PREDICTED: cysteine proteinase inhibitor 5-like [Cucumis melo] >KAA0025519.1 cys...	[more]
KAE8647756.1	6.8e-15	53.26	hypothetical protein Csa_003034 [Cucumis sativus]	[more]
NP_001267677.1	6.8e-15	53.26	cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein prote...	[more]
KAE8647459.1	2.6e-14	43.88	hypothetical protein Csa_003259 [Cucumis sativus]	[more]
KAE8648197.1	7.6e-14	52.22	hypothetical protein Csa_018520 [Cucumis sativus]	[more]

BLAST of Bhi03G001029 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SJM4	1.2e-17	48.45	Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B2L7	1.2e-17	48.45	cysteine proteinase inhibitor 5-like OS=Cucumis melo OX=3656 GN=LOC103485290 PE=...	[more]
O80389	3.3e-15	53.26	Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A0A0KIY5	2.1e-14	44.79	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483248 PE...	[more]
A0A0A0KF46	4.8e-14	46.60	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483245 PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 3..99 e-value: 8.4E-18 score: 66.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 6..99
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 6..99
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 3..98
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 18..98 e-value: 3.8E-15 score: 56.0
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 13..73 e-value: 3.19004E-5 score: 37.4536

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M001029	Bhi03M001029	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity