Bhi03G000958 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000958
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Cytochrome P450 family protein
Location	chr3: 25545330 .. 25545986 (+)
RNA-Seq Expression	Bhi03G000958
Synteny	Bhi03G000958

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTGATTTGGATCATTATTATTATCATTCATCTGCTTGCTCTTCCACTCGTCCTTTGGCAATGGCTGCATAGGATTAACAAAACCAAGAAATTACTGCCTCCTGGTCCTAAAGGTTTCCCCATTTTCGGTAGCCTTCATTTGTTAGGAAAGCTTCCTCATAGAGATCTTCACCGCTTGTCTCAAAAATATGGACCCATAATGCACATGAAACTAGGCCTTGTAAACACCATTGTTGTGTCATCCTCTGAAGCCGCAGAGCTGTTTCTGAAAACCCATGATCTTGATTTTGCAAATCGCCCACCTAATGGGGTTTTAAAGCACATGTATTTTGGCCAAAACAGCATCATAGTTGCAAAATATGGGCCTTATTTGCAGAACGTGCGCAAAATGTTAACTCTGGAGTTGCTTAGCAGCCACAAACTCGATTCATTCAAAGCAATGAGAATGGAAGAAGTTGGGCTGTTGATTGAGGAGCTTCGTGAGGCTGCAAAAGGTGGCATGGTTGTAAATCTTTCTTCCAAAGTATATTCTCTCAATGCAAATATGATATGTCTTATGATCTTTGGGAGGAAATTTGAAGATAAAGAAATTGATGAGAGGGGTTTTAAGGGGGATGATACAAGAGACCTTCAAACTACTTGGAGCTTTTAA

mRNA sequence

ATGGGTTTGATTTGGATCATTATTATTATCATTCATCTGCTTGCTCTTCCACTCGTCCTTTGGCAATGGCTGCATAGGATTAACAAAACCAAGAAATTACTGCCTCCTGGTCCTAAAGGTTTCCCCATTTTCGGTAGCCTTCATTTGTTAGGAAAGCTTCCTCATAGAGATCTTCACCGCTTGTCTCAAAAATATGGACCCATAATGCACATGAAACTAGGCCTTGTAAACACCATTGTTGTGTCATCCTCTGAAGCCGCAGAGCTGTTTCTGAAAACCCATGATCTTGATTTTGCAAATCGCCCACCTAATGGGGTTTTAAAGCACATGTATTTTGGCCAAAACAGCATCATAGTTGCAAAATATGGGCCTTATTTGCAGAACGTGCGCAAAATGTTAACTCTGGAGTTGCTTAGCAGCCACAAACTCGATTCATTCAAAGCAATGAGAATGGAAGAAGTTGGGCTGTTGATTGAGGAGCTTCGTGAGGCTGCAAAAGGTGGCATGGTTGTAAATCTTTCTTCCAAAGTATATTCTCTCAATGCAAATATGATATGTCTTATGATCTTTGGGAGGAAATTTGAAGATAAAGAAATTGATGAGAGGGGTTTTAAGGGGGATGATACAAGAGACCTTCAAACTACTTGGAGCTTTTAA

Coding sequence (CDS)

ATGGGTTTGATTTGGATCATTATTATTATCATTCATCTGCTTGCTCTTCCACTCGTCCTTTGGCAATGGCTGCATAGGATTAACAAAACCAAGAAATTACTGCCTCCTGGTCCTAAAGGTTTCCCCATTTTCGGTAGCCTTCATTTGTTAGGAAAGCTTCCTCATAGAGATCTTCACCGCTTGTCTCAAAAATATGGACCCATAATGCACATGAAACTAGGCCTTGTAAACACCATTGTTGTGTCATCCTCTGAAGCCGCAGAGCTGTTTCTGAAAACCCATGATCTTGATTTTGCAAATCGCCCACCTAATGGGGTTTTAAAGCACATGTATTTTGGCCAAAACAGCATCATAGTTGCAAAATATGGGCCTTATTTGCAGAACGTGCGCAAAATGTTAACTCTGGAGTTGCTTAGCAGCCACAAACTCGATTCATTCAAAGCAATGAGAATGGAAGAAGTTGGGCTGTTGATTGAGGAGCTTCGTGAGGCTGCAAAAGGTGGCATGGTTGTAAATCTTTCTTCCAAAGTATATTCTCTCAATGCAAATATGATATGTCTTATGATCTTTGGGAGGAAATTTGAAGATAAAGAAATTGATGAGAGGGGTTTTAAGGGGGATGATACAAGAGACCTTCAAACTACTTGGAGCTTTTAA

Protein sequence

MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMICLMIFGRKFEDKEIDERGFKGDDTRDLQTTWSF

Homology

[Close]

BLAST of Bhi03G000958 vs. TAIR 10
Match: AT3G26200.1 (cytochrome P450, family 71, subfamily B, polypeptide 22 )

HSP 1 Score: 143.3 bits (360), Expect = 2.3e-34
Identity = 78/189 (41.27%), Postives = 116/189 (61.38%), Query Frame = 0


Query: 8   IIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGP 67
           I +  LL LPL L  +  +++ +K  LPPGP G PI G+LH LGK  HR  H+LSQ YGP
Sbjct: 3   ISLYFLLLLPLFL-IFFKKLSPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGP 62

Query: 68  IMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPYLQ 127
           +M +  G+V  +VVS+ EAAE  LKTHDL+   RP     K   +    I  A+YG   +
Sbjct: 63  VMFLHFGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWR 122

Query: 128 NVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMICL 187
            +RK+  LEL SS KL +F+ +R EE  +L+ +L ++A+   +V+L   ++S  A+++C 
Sbjct: 123 EMRKLAMLELFSSKKLKAFRYIREEESEVLVNKLSKSAETRTMVDLRKALFSYTASIVCR 182

Query: 188 MIFGRKFED 197
           + FG+ F +
Sbjct: 183 LAFGQNFHE 190

[Close]

BLAST of Bhi03G000958 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 142.9 bits (359), Expect = 3.0e-34
Identity = 83/205 (40.49%), Postives = 122/205 (59.51%), Query Frame = 0


Query: 7   IIIIIHLLALPLVLWQWLHRINKTKKL-LPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKY 66
           I++   L++L  ++     + NKT K  LPP P   PI G+LH L  LPHR  H+LS KY
Sbjct: 3   ILLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKY 62

Query: 67  GPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPY 126
           GP++ ++LG V  +V+SSSEAAE  LKT+DL+  +RP       + +G   I  A YG Y
Sbjct: 63  GPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEY 122

Query: 127 LQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMI 186
            + VRK+  +EL SS K+ SF+ +R EEV  +++++ E+A     V+LS   +SL A++I
Sbjct: 123 WREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASII 182

Query: 187 CLMIFGRKFEDKEIDERGFKGDDTR 211
           C +  G+ F     +E GF  D  R
Sbjct: 183 CRVALGQNF-----NESGFVIDQDR 202

[Close]

BLAST of Bhi03G000958 vs. TAIR 10
Match: AT3G26190.1 (cytochrome P450, family 71, subfamily B, polypeptide 21 )

HSP 1 Score: 137.9 bits (346), Expect = 9.8e-33
Identity = 76/189 (40.21%), Postives = 115/189 (60.85%), Query Frame = 0


Query: 8   IIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGP 67
           I +  LL LPL L  +  R+  +K  LPPGP   PI G+LH LGK  HR  ++LSQ+YGP
Sbjct: 3   IFLCFLLLLPLFL-VFYKRLLPSKGKLPPGPISLPIIGNLHQLGKSLHRSFYKLSQEYGP 62

Query: 68  IMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPYLQ 127
           +M ++ G+V  +V S+ EAAE  LKTHDL+   RP         +    I  A+YG   +
Sbjct: 63  VMFLRFGVVPVVVFSTKEAAEEVLKTHDLETCTRPKLSATGLFTYNFKDIGFAQYGEDWR 122

Query: 128 NVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMICL 187
            +RK+  LEL SS KL +F+ +R EE  LL++++ E+A+   +V+L   ++S  A+++C 
Sbjct: 123 EMRKLAMLELFSSKKLKAFRYIREEESELLVKKVTESAQTQTLVDLRKALFSYTASIVCR 182

Query: 188 MIFGRKFED 197
           + FG+ F +
Sbjct: 183 LAFGQNFHE 190

[Close]

BLAST of Bhi03G000958 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 132.9 bits (333), Expect = 3.2e-31
Identity = 77/197 (39.09%), Postives = 118/197 (59.90%), Query Frame = 0


Query: 6   IIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKY 65
           I +  + LL L LV   +  +   +K  LPPGP   PI G+LH L  LPH+ L  L + +
Sbjct: 3   IFLCFLLLLLLLLVTIIFTRKSQSSKLKLPPGPPKLPIIGNLHYLNGLPHKCLLNLWKIH 62

Query: 66  GPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPY 125
           GP+M ++LG V  +V+SS++AAE  LKTHDLD  +RP     K + +    I  A YG  
Sbjct: 63  GPVMQLQLGYVPLVVISSNQAAEEVLKTHDLDCCSRPETIASKTISYNFKDIGFAPYGEE 122

Query: 126 LQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMI 185
            + +RK+  +EL S  K +SF+ +R EE  LL+++L EA++    VNL   +++L+A+++
Sbjct: 123 WRALRKLAVIELFSLKKFNSFRYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSASIV 182

Query: 186 CLMIFGRKFEDKE-IDE 202
           C + FG+   + E IDE
Sbjct: 183 CRLAFGQNLHESEFIDE 199

[Close]

BLAST of Bhi03G000958 vs. TAIR 10
Match: AT2G24180.1 (cytochrome p450 71b6 )

HSP 1 Score: 131.3 bits (329), Expect = 9.2e-31
Identity = 74/202 (36.63%), Postives = 114/202 (56.44%), Query Frame = 0


Query: 3   LIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLS 62
           L W+++++I     PL+++  L    ++ K LPPGP   PI G++H LG LPHR L  LS
Sbjct: 13  LPWLLLLLIP----PLLIFFLL----RSPKNLPPGPPRLPILGNIHQLGSLPHRSLRDLS 72

Query: 63  QKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKY 122
            KYGP++ + LG V T+VV S E AE  LK HD +   RP   + K  ++    +   K+
Sbjct: 73  LKYGPVITVYLGSVRTVVVHSPETAEEVLKLHDSECCTRPKLSITKSFFYDGLGLGFTKW 132

Query: 123 GPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNA 182
           G Y ++VRK+  LEL S  + +SF+ +R EE+  L+    ++A  G  V+L++ +    A
Sbjct: 133 GDYYRDVRKLCVLELFSVKRANSFRNIREEELSRLVNSFSDSASSGSSVDLTANLAKFVA 192

Query: 183 NMICLMIFGRKFEDKEIDERGF 205
           +  C M FG  F+   +D   F
Sbjct: 193 SFTCRMAFGLSFQGSGMDNETF 206

[Close]

BLAST of Bhi03G000958 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.9e-58
Identity = 118/204 (57.84%), Postives = 155/204 (75.98%), Query Frame = 0


Query: 3   LIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLS 62
           ++W I   I LLAL  +L  W       KK LPPGP+GFPIFGSLHLLG+ P++DLHRL+
Sbjct: 1   MVW-IWATIGLLALVHILQAWW---KNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLA 60

Query: 63  QKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKY 122
           +KYG IM+M+LGL+ TIV+SS EAAELFLKTHDL FA+RPP+   KH+ FGQ ++I ++Y
Sbjct: 61  RKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEY 120

Query: 123 GPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKG-GMVVNLSSKVYSLN 182
           G Y ++ RKM T+ELLS+HK++SFK+MR EEV L +E +R AA   G+ V+LS KV SL+
Sbjct: 121 GAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLS 180

Query: 183 ANMICLMIFGRKFEDKEIDERGFK 206
            +M C M+ G+K+ D+E DERGFK
Sbjct: 181 VDMSCRMVLGKKYRDEEFDERGFK 200

[Close]

BLAST of Bhi03G000958 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.1e-40
Identity = 91/193 (47.15%), Postives = 126/193 (65.28%), Query Frame = 0


Query: 14  LALPLVL-------WQWLHRINKTKK--LLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQK 73
           LA PL+        W  L  I+  K+   LPPGP+G PI GSLH LG LPHR L  L++K
Sbjct: 4   LAYPLLFVLLGALSWWILPIISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKK 63

Query: 74  YGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGP 133
           YGPIM M+LG V TIVVSS +AAELFLKTHD  FA+RP     ++M +G   +    YGP
Sbjct: 64  YGPIMSMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGP 123

Query: 134 YLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANM 193
           + +N+RK + LELL+  K++SF  MR EE+G +++ ++EA+    VV+LS+KV ++  NM
Sbjct: 124 HWRNIRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENM 183

Query: 194 ICLMIFGRKFEDK 198
              ++ GR  +D+
Sbjct: 184 TYRLLLGRTKDDR 196

[Close]

BLAST of Bhi03G000958 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 3.0e-34
Identity = 76/193 (39.38%), Postives = 122/193 (63.21%), Query Frame = 0


Query: 9   IIIHLLALPLVLWQWLHRIN---KTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKY 68
           I++ LL L + L  +L ++N   + K  LPP P   PI G+LH LG LPHR L  L+ + 
Sbjct: 3   ILVSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANEL 62

Query: 69  GPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPY 128
           GP++ + LG + T++VS++E AE  LKTHDL FA+RP     + +++    +  + YG Y
Sbjct: 63  GPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEY 122

Query: 129 LQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMI 188
            + VRK+  LELLS  +++S++++R EEVGL++E + ++   G  VNLS  +  L++  I
Sbjct: 123 WRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTI 182

Query: 189 CLMIFGRKFEDKE 199
             + FG+K+E +E
Sbjct: 183 TRVAFGKKYEGEE 195

[Close]

BLAST of Bhi03G000958 vs. ExPASy Swiss-Prot
Match: Q9LTM1 (Cytochrome P450 71B22 OS=Arabidopsis thaliana OX=3702 GN=CYP71B22 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.3e-33
Identity = 78/189 (41.27%), Postives = 116/189 (61.38%), Query Frame = 0


Query: 8   IIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGP 67
           I +  LL LPL L  +  +++ +K  LPPGP G PI G+LH LGK  HR  H+LSQ YGP
Sbjct: 3   ISLYFLLLLPLFL-IFFKKLSPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGP 62

Query: 68  IMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPYLQ 127
           +M +  G+V  +VVS+ EAAE  LKTHDL+   RP     K   +    I  A+YG   +
Sbjct: 63  VMFLHFGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWR 122

Query: 128 NVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMICL 187
            +RK+  LEL SS KL +F+ +R EE  +L+ +L ++A+   +V+L   ++S  A+++C 
Sbjct: 123 EMRKLAMLELFSSKKLKAFRYIREEESEVLVNKLSKSAETRTMVDLRKALFSYTASIVCR 182

Query: 188 MIFGRKFED 197
           + FG+ F +
Sbjct: 183 LAFGQNFHE 190

[Close]

BLAST of Bhi03G000958 vs. ExPASy Swiss-Prot
Match: O65788 (Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 4.3e-33
Identity = 83/205 (40.49%), Postives = 122/205 (59.51%), Query Frame = 0


Query: 7   IIIIIHLLALPLVLWQWLHRINKTKKL-LPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKY 66
           I++   L++L  ++     + NKT K  LPP P   PI G+LH L  LPHR  H+LS KY
Sbjct: 3   ILLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKY 62

Query: 67  GPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPY 126
           GP++ ++LG V  +V+SSSEAAE  LKT+DL+  +RP       + +G   I  A YG Y
Sbjct: 63  GPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEY 122

Query: 127 LQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMI 186
            + VRK+  +EL SS K+ SF+ +R EEV  +++++ E+A     V+LS   +SL A++I
Sbjct: 123 WREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASII 182

Query: 187 CLMIFGRKFEDKEIDERGFKGDDTR 211
           C +  G+ F     +E GF  D  R
Sbjct: 183 CRVALGQNF-----NESGFVIDQDR 202

[Close]

BLAST of Bhi03G000958 vs. NCBI nr
Match: XP_038897271.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 1.1e-86
Identity = 172/208 (82.69%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHR-INKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLH 60
           MGLIW+III IH+L +PLV WQWL R  NKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLH
Sbjct: 1   MGLIWMIIITIHVLVVPLVFWQWLQRNNNKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLH 60

Query: 61  RLSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIV 120
           +LSQKYGPIMH+KLGLVNTIVVSS +AAELFLKTHDLDFANRPPN V KH+YFGQNSI V
Sbjct: 61  QLSQKYGPIMHIKLGLVNTIVVSSPKAAELFLKTHDLDFANRPPNEVFKHIYFGQNSIAV 120

Query: 121 AKYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYS 180
           + YGPYL+N+RK+ TLELLSSHKLDSFK MR EEVGLLIEELR AA+ G+VVNL+SKV S
Sbjct: 121 SDYGPYLRNMRKIHTLELLSSHKLDSFKPMRREEVGLLIEELRGAARRGVVVNLTSKVSS 180

Query: 181 LNANMICLMIFGRKFE-DKEID-ERGFK 206
           L ANMICLMIFGRKFE DKE+D E GFK
Sbjct: 181 LIANMICLMIFGRKFEDDKELDHEMGFK 208

[Close]

BLAST of Bhi03G000958 vs. NCBI nr
Match: KAA0036258.1 (cytochrome P450 family protein [Cucumis melo var. makuwa] >TYK12652.1 cytochrome P450 family protein [Cucumis melo var. makuwa])

HSP 1 Score: 288.5 bits (737), Expect = 4.8e-74
Identity = 139/178 (78.09%), Postives = 162/178 (91.01%), Query Frame = 0


Query: 28  NKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGPIMHMKLGLVNTIVVSSSEAA 87
           NKTKKLLPPGPKGFPIFGSLH+LGKLP    HRL QKYG IMH+KLGLVNTIV+SSSEAA
Sbjct: 3   NKTKKLLPPGPKGFPIFGSLHILGKLP----HRLFQKYGAIMHIKLGLVNTIVLSSSEAA 62

Query: 88  ELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPYLQNVRKMLTLELLSSHKLDSFK 147
           ELFLKTHDLDFAN PPN   KH+ FGQ+S++VAKYGPY++NVRKM T+ELL+SHKL+SFK
Sbjct: 63  ELFLKTHDLDFANHPPNDAFKHISFGQSSLVVAKYGPYVRNVRKMCTVELLNSHKLNSFK 122

Query: 148 AMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMICLMIFGRKFEDKEIDERGFK 206
           +MRMEEVGL IEELREAA+ G++VNL+SK+ SL+ANMICL +FGRK+EDKE+DE+GFK
Sbjct: 123 SMRMEEVGLFIEELREAARSGLLVNLTSKLSSLSANMICLTVFGRKYEDKELDEKGFK 176

[Close]

BLAST of Bhi03G000958 vs. NCBI nr
Match: XP_022950433.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 280.8 bits (717), Expect = 1.0e-71
Identity = 145/206 (70.39%), Postives = 170/206 (82.52%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHR 60
           M  IWIIII   +  L L+ W WL R N+++K LPPGPKGFPI GSLHLLGKLPHRDLHR
Sbjct: 1   MAWIWIIII---MFTLVLLFWAWLGR-NQSQK-LPPGPKGFPILGSLHLLGKLPHRDLHR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVA 120
           LSQ YGPIMHMKLGLVNTIVVSS EAAELFLKTHDL FA+R PN V KH+ FGQ +++ A
Sbjct: 61  LSQTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRAPNEVFKHISFGQKTMVFA 120

Query: 121 KYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELR-EAAKGGMVVNLSSKVYS 180
           KYGPY +N+ KM  LELLS+HK+DSFK+MR +E+G+LI++LR +AA   +VVNL+SK+YS
Sbjct: 121 KYGPYWRNMHKMCVLELLSNHKIDSFKSMRRQELGVLIDQLRDDAANSRVVVNLTSKIYS 180

Query: 181 LNANMICLMIFGRKFEDKEIDERGFK 206
           L  NM CLM+ GRKFEDKEIDERGFK
Sbjct: 181 LTINMACLMVLGRKFEDKEIDERGFK 201

[Close]

BLAST of Bhi03G000958 vs. NCBI nr
Match: XP_023544864.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278.5 bits (711), Expect = 5.0e-71
Identity = 142/205 (69.27%), Postives = 166/205 (80.98%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHR 60
           M  IW+III   + AL L+ W W+ R NK+KK LPPGPKGFPI GSLHLLGKLPHRDLHR
Sbjct: 1   MAWIWMIII---MFALVLLFWAWMER-NKSKK-LPPGPKGFPIMGSLHLLGKLPHRDLHR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVA 120
           LSQ YGPIMHMKLG+VNTIVVSS EAAELFLKTHD+ FA+R PN V KH+ F Q +I  A
Sbjct: 61  LSQTYGPIMHMKLGIVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFA 120

Query: 121 KYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSL 180
           KYGPY +N+RKM  LELLS+HK +  ++MR +E+G  I +LR+AAK GMVVN+SSK+YSL
Sbjct: 121 KYGPYWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRDAAKSGMVVNISSKIYSL 180

Query: 181 NANMICLMIFGRKFEDKEIDERGFK 206
            ANM CLMI GR+FEDKE+DE GFK
Sbjct: 181 TANMACLMIIGRRFEDKELDEMGFK 200

[Close]

BLAST of Bhi03G000958 vs. NCBI nr
Match: XP_022950431.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 277.3 bits (708), Expect = 1.1e-70
Identity = 142/205 (69.27%), Postives = 165/205 (80.49%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHR 60
           M  IW+III   + AL L+ W W+ R NK+KK LPPGPKGFPI GSLHLLGKLPHRDLHR
Sbjct: 1   MAWIWMIII---MFALVLLFWAWMER-NKSKK-LPPGPKGFPIMGSLHLLGKLPHRDLHR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVA 120
           LSQ YGPIMHMKLGLVNTIVVSS EAAELFLKTHD+ FA+R PN V KH+ F Q +I  A
Sbjct: 61  LSQTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFA 120

Query: 121 KYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSL 180
           KYGPY +N+RKM  LELLS+HK +  ++MR +E+G  I +LR+AA  GMVVN+SSK+YSL
Sbjct: 121 KYGPYWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRDAANSGMVVNISSKIYSL 180

Query: 181 NANMICLMIFGRKFEDKEIDERGFK 206
            ANM CLMI GR+FEDKE+DE GFK
Sbjct: 181 TANMACLMILGRRFEDKELDEMGFK 200

[Close]

BLAST of Bhi03G000958 vs. ExPASy TrEMBL
Match: A0A5D3CMT9 (Cytochrome P450 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002400 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.3e-74
Identity = 139/178 (78.09%), Postives = 162/178 (91.01%), Query Frame = 0


Query: 28  NKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHRLSQKYGPIMHMKLGLVNTIVVSSSEAA 87
           NKTKKLLPPGPKGFPIFGSLH+LGKLP    HRL QKYG IMH+KLGLVNTIV+SSSEAA
Sbjct: 3   NKTKKLLPPGPKGFPIFGSLHILGKLP----HRLFQKYGAIMHIKLGLVNTIVLSSSEAA 62

Query: 88  ELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVAKYGPYLQNVRKMLTLELLSSHKLDSFK 147
           ELFLKTHDLDFAN PPN   KH+ FGQ+S++VAKYGPY++NVRKM T+ELL+SHKL+SFK
Sbjct: 63  ELFLKTHDLDFANHPPNDAFKHISFGQSSLVVAKYGPYVRNVRKMCTVELLNSHKLNSFK 122

Query: 148 AMRMEEVGLLIEELREAAKGGMVVNLSSKVYSLNANMICLMIFGRKFEDKEIDERGFK 206
           +MRMEEVGL IEELREAA+ G++VNL+SK+ SL+ANMICL +FGRK+EDKE+DE+GFK
Sbjct: 123 SMRMEEVGLFIEELREAARSGLLVNLTSKLSSLSANMICLTVFGRKYEDKELDEKGFK 176

[Close]

BLAST of Bhi03G000958 vs. ExPASy TrEMBL
Match: A0A6J1GEV6 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453536 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 4.9e-72
Identity = 145/206 (70.39%), Postives = 170/206 (82.52%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHR 60
           M  IWIIII   +  L L+ W WL R N+++K LPPGPKGFPI GSLHLLGKLPHRDLHR
Sbjct: 1   MAWIWIIII---MFTLVLLFWAWLGR-NQSQK-LPPGPKGFPILGSLHLLGKLPHRDLHR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVA 120
           LSQ YGPIMHMKLGLVNTIVVSS EAAELFLKTHDL FA+R PN V KH+ FGQ +++ A
Sbjct: 61  LSQTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRAPNEVFKHISFGQKTMVFA 120

Query: 121 KYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELR-EAAKGGMVVNLSSKVYS 180
           KYGPY +N+ KM  LELLS+HK+DSFK+MR +E+G+LI++LR +AA   +VVNL+SK+YS
Sbjct: 121 KYGPYWRNMHKMCVLELLSNHKIDSFKSMRRQELGVLIDQLRDDAANSRVVVNLTSKIYS 180

Query: 181 LNANMICLMIFGRKFEDKEIDERGFK 206
           L  NM CLM+ GRKFEDKEIDERGFK
Sbjct: 181 LTINMACLMVLGRKFEDKEIDERGFK 201

[Close]

BLAST of Bhi03G000958 vs. ExPASy TrEMBL
Match: A0A6J1GET5 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 5.4e-71
Identity = 142/205 (69.27%), Postives = 165/205 (80.49%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHR 60
           M  IW+III   + AL L+ W W+ R NK+KK LPPGPKGFPI GSLHLLGKLPHRDLHR
Sbjct: 1   MAWIWMIII---MFALVLLFWAWMER-NKSKK-LPPGPKGFPIMGSLHLLGKLPHRDLHR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVA 120
           LSQ YGPIMHMKLGLVNTIVVSS EAAELFLKTHD+ FA+R PN V KH+ F Q +I  A
Sbjct: 61  LSQTYGPIMHMKLGLVNTIVVSSPEAAELFLKTHDVVFASRAPNEVFKHVSFSQKTIAFA 120

Query: 121 KYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSL 180
           KYGPY +N+RKM  LELLS+HK +  ++MR +E+G  I +LR+AA  GMVVN+SSK+YSL
Sbjct: 121 KYGPYWRNMRKMCILELLSNHKANYLQSMRRQELGFTIHQLRDAANSGMVVNISSKIYSL 180

Query: 181 NANMICLMIFGRKFEDKEIDERGFK 206
            ANM CLMI GR+FEDKE+DE GFK
Sbjct: 181 TANMACLMILGRRFEDKELDEMGFK 200

[Close]

BLAST of Bhi03G000958 vs. ExPASy TrEMBL
Match: A0A6J1GET0 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 5.9e-70
Identity = 143/205 (69.76%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHR 60
           M  IWIIII   + AL L+ W WL + NK K  LPPGPKGFPI GSLHLLGKLPHRD+ R
Sbjct: 1   MAWIWIIII---MFALVLLFWPWLLK-NKGKN-LPPGPKGFPILGSLHLLGKLPHRDIQR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVA 120
           LSQ+YGPIMHMKLGLVNTIVVSS EAA LFLKTHDL FA+RP N V KHM +G+ S++ +
Sbjct: 61  LSQRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFS 120

Query: 121 KYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSL 180
           KYG Y +N+RK+ TLELLS+ K++ FK+MR EE+ LLI+ELREAAK  +VVNLSSKV SL
Sbjct: 121 KYGHYWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSL 180

Query: 181 NANMICLMIFGRKFEDKEIDERGFK 206
           +ANM CLMIFG+KFED+++DERGFK
Sbjct: 181 SANMTCLMIFGKKFEDRDLDERGFK 200

[Close]

BLAST of Bhi03G000958 vs. ExPASy TrEMBL
Match: A0A6J1IU54 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.5e-68
Identity = 141/205 (68.78%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 1   MGLIWIIIIIIHLLALPLVLWQWLHRINKTKKLLPPGPKGFPIFGSLHLLGKLPHRDLHR 60
           M  IW+III   + AL L+   WL + NK K  LPPGPKGFPI GSLHLLGKLPHRD+ R
Sbjct: 1   MAWIWMIII---MFALVLLFRSWLLK-NKAKN-LPPGPKGFPILGSLHLLGKLPHRDIQR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSSEAAELFLKTHDLDFANRPPNGVLKHMYFGQNSIIVA 120
           LSQ+YGPIMHMKLGLVNTIVVSS EAA LFLKTHDL FA+RP N V KHM +G+ S++ +
Sbjct: 61  LSQRYGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFS 120

Query: 121 KYGPYLQNVRKMLTLELLSSHKLDSFKAMRMEEVGLLIEELREAAKGGMVVNLSSKVYSL 180
           KYG Y +N+RK+ TLELLS+ K++ FK+MR EE+ LLI+ELREAAK  +VVNLSSKV SL
Sbjct: 121 KYGHYWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSL 180

Query: 181 NANMICLMIFGRKFEDKEIDERGFK 206
           +ANM CLMIFG+KFED+++DERGFK
Sbjct: 181 SANMTCLMIFGKKFEDRDLDERGFK 200

The following BLAST results are available for this feature:

BLAST of Bhi03G000958 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26200.1	2.3e-34	41.27	cytochrome P450, family 71, subfamily B, polypeptide 22	[more]
AT1G13080.1	3.0e-34	40.49	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT3G26190.1	9.8e-33	40.21	cytochrome P450, family 71, subfamily B, polypeptide 21	[more]
AT3G26210.1	3.2e-31	39.09	cytochrome P450, family 71, subfamily B, polypeptide 23	[more]
AT2G24180.1	9.2e-31	36.63	cytochrome p450 71b6	[more]

BLAST of Bhi03G000958 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q5V6	2.9e-58	57.84	Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1	[more]
H2DH18	2.1e-40	47.15	Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
P24465	3.0e-34	39.38	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
Q9LTM1	3.3e-33	41.27	Cytochrome P450 71B22 OS=Arabidopsis thaliana OX=3702 GN=CYP71B22 PE=2 SV=1	[more]
O65788	4.3e-33	40.49	Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2	[more]

BLAST of Bhi03G000958 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897271.1	1.1e-86	82.69	cytochrome P450 71AU50-like [Benincasa hispida]	[more]
KAA0036258.1	4.8e-74	78.09	cytochrome P450 family protein [Cucumis melo var. makuwa] >TYK12652.1 cytochrome...	[more]
XP_022950433.1	1.0e-71	70.39	cytochrome P450 CYP736A12-like [Cucurbita moschata]	[more]
XP_023544864.1	5.0e-71	69.27	cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022950431.1	1.1e-70	69.27	cytochrome P450 CYP736A12-like [Cucurbita moschata]	[more]

BLAST of Bhi03G000958 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CMT9	2.3e-74	78.09	Cytochrome P450 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1GEV6	4.9e-72	70.39	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453536 PE=...	[more]
A0A6J1GET5	5.4e-71	69.27	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453534 PE=...	[more]
A0A6J1GET0	5.9e-70	69.76	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=...	[more]
A0A6J1IU54	2.5e-68	68.78	cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 62..81 score: 31.51 coord: 180..198 score: 30.27 coord: 86..107 score: 25.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 35..196 e-value: 2.6E-24 score: 85.8
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 24..216 e-value: 8.8E-36 score: 125.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 26..197
None	No IPR available	PANTHER	PTHR47943:SF2	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 5..205
None	No IPR available	PANTHER	PTHR47943	CYTOCHROME P450 93A3-LIKE	coord: 5..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000958	Bhi03M000958	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen