Bhi03G000955 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000955
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptioncytochrome P450 CYP736A12-like
Locationchr3: 25358928 .. 25360849 (+)
RNA-Seq ExpressionBhi03G000955
SyntenyBhi03G000955
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTGGATTTGGGCCACAATTGCACTATTACTTCTTTCAAACTACCTCTTTCAAACATGGCAATTCAACAAGAAGAGCAAGGGCAAGAAATTACCTCCTGGCCCAAAAGGGTTTCCTATTATTGGCAGCCTCAATTTGCTAAAAGAGTTTCCTCATAGAGATCTTCATCAATTGTCTCAAAAATATGGTCCCATAATGCACATGAAATTGGGACTTGTAAACACAATTGTTGTCTCTTCCCCAACAGCTGCTGAGCTTTTTCTCAAAACCCATGACCTTATTTTTGCAAGTAGGCCCCTCACTATGGTCTCAAAATTCCTGTCTTATGGCCAAAAGAATTTGGTTTTTGCTCAATATGGATCTTATTGGAGGAATATACGTAAAATGTGTACGCTTGAATTGCTTAGTAACCACAAGATCAATTCTTTCAAATCCATGAGAAAGGAAGAACTTGGATTGTTGATTGAGCATCTCAAAGAGGCTGCAAATGATGGTGTTGCTATTAATCTTAGTTCTAAAGTCGTGTCACTCAGCACAGATATGACTTGTTTGATGGTTTTTGGGAGGAAATTTGGAGACGAAGAGTTGGATGATAGAGGTTTTAAGGCTATGATGCAAGAGGTGATGCAATTAATCGCTGCTCCTAATTTGGGAGATTTTATTCCTTTTATTGCAAGACTTGATCTCCAAGGGTTGACACGTCGGATGAAAAATGTTAGCAAAGTGTTTGATGGGTTCTTTGAGAGGATTATTGATGAACATCTTAACTCTATGGGTGGAAAAAGAACTAAAGATTTTTTAGATGTCATGTTGGATCTAATGGACTCTGTGGATACTCATGAGTATCGAATTGATCGTTCCTGCGTCAAAGCAATAATAATGGTGAGAATTTGAACCCTCTGATCTCTTAGTGAAAGACAAATATCTTAATAATTCTTCGTTAAACTATGGTCAAGTTGATTTACTTTTAATAGAATATGCCTATTTGAAAAATGATTTTGGAAGTGCATAAAAATACTTTTTAAAACAGTTTTTAAAGTCATTTGTGATAATTTGCACATGAAAATTTATTATTATTATTTTTTAAGTTTAAATACTATTTTGTCTCTACATGTATTTTTACATTGATTTATTTTGGTCAATTTATACTTTCAAAAGATTCATCTTGGTGATCTCTAAACTTTAGTTCATTTTCATTTTGTACTTTCAAAATTTTCATTTAGGTCATTATATTTTCAAATTTGATTCATTTTAATCTTTGAACATTTAAAAAGCGTCAATTTTAATCTCTTACAAATGGTAAAAAATGAAAGTGAAAGAATTACCAAATTAGTTACTTTTAAAGACGGAATGAACTAAAAACAAAAACAAAAGTAAAAAGATTAAACTAGCATTTAAACAATTTTTTTAAAATATGGCATAAAGTTGTGGGATGTGTTTCAATGAGAGTTGGTTTATTACATATTCTTATTGGTACTATTTCTTCATAGGATATGCTTGGTGCTGCAATGGACACTGCAGCAACAGTAATTGGATGGGCACTTCCTGAGCTCATAAGACATCCACATGTAATGAAAAAATTGCAAGATGAAATAGAAAATGTGGTGGGGTTGGATAGAATGGTGCAAGAATCTGATTTAGTTCACTTGGAATATTTGGACATGGTAATGAAGGAAATCCTTAGGCTGTATCCACCAGCTCCATTGTTGGTTCCACATGAATCTATTGAAGAATGCATTGTTGAAGACTTTTTCATTCCAAAAAAATCACGTATAATAGTGAATGCATGGGCAATTGGACGGAACCCAAGTGCTTGGATTGATCCAGAAATGTTCTATCCAGAGAGATTTATTGGTAGCAAAGTGGATGTAAAGGGAAGAGATTTTCAATTAATTCCATTTGGGTTTGACTGTGGTTAG

mRNA sequence

ATGGCTTGGATTTGGGCCACAATTGCACTATTACTTCTTTCAAACTACCTCTTTCAAACATGGCAATTCAACAAGAAGAGCAAGGGCAAGAAATTACCTCCTGGCCCAAAAGGGTTTCCTATTATTGGCAGCCTCAATTTGCTAAAAGAGTTTCCTCATAGAGATCTTCATCAATTGTCTCAAAAATATGGTCCCATAATGCACATGAAATTGGGACTTGTAAACACAATTGTTGTCTCTTCCCCAACAGCTGCTGAGCTTTTTCTCAAAACCCATGACCTTATTTTTGCAAGTAGGCCCCTCACTATGGTCTCAAAATTCCTGTCTTATGGCCAAAAGAATTTGGTTTTTGCTCAATATGGATCTTATTGGAGGAATATACGTAAAATGTGTACGCTTGAATTGCTTAGTAACCACAAGATCAATTCTTTCAAATCCATGAGAAAGGAAGAACTTGGATTGTTGATTGAGCATCTCAAAGAGGCTGCAAATGATGGTGTTGCTATTAATCTTAGTTCTAAAGTCGTGTCACTCAGCACAGATATGACTTGTTTGATGGTTTTTGGGAGGAAATTTGGAGACGAAGAGTTGGATGATAGAGGTTTTAAGGCTATGATGCAAGAGGTGATGCAATTAATCGCTGCTCCTAATTTGGGAGATTTTATTCCTTTTATTGCAAGACTTGATCTCCAAGGGTTGACACGTCGGATGAAAAATGTTAGCAAAGTGTTTGATGGGTTCTTTGAGAGGATTATTGATGAACATCTTAACTCTATGGGTGGAAAAAGAACTAAAGATTTTTTAGATGTCATGTTGGATCTAATGGACTCTGTGGATACTCATGAGTATCGAATTGATCGTTCCTGCGTCAAAGCAATAATAATGGATATGCTTGGTGCTGCAATGGACACTGCAGCAACAGTAATTGGATGGGCACTTCCTGAGCTCATAAGACATCCACATGTAATGAAAAAATTGCAAGATGAAATAGAAAATGTGGTGGGGTTGGATAGAATGGTGCAAGAATCTGATTTAGTTCACTTGGAATATTTGGACATGGTAATGAAGGAAATCCTTAGGCTGTATCCACCAGCTCCATTGTTGGTTCCACATGAATCTATTGAAGAATGCATTGTTGAAGACTTTTTCATTCCAAAAAAATCACGTATAATAGTGAATGCATGGGCAATTGGACGGAACCCAAGTGCTTGGATTGATCCAGAAATGTTCTATCCAGAGAGATTTATTGGTAGCAAAGTGGATGTAAAGGGAAGAGATTTTCAATTAATTCCATTTGGGTTTGACTGTGGTTAG

Coding sequence (CDS)

ATGGCTTGGATTTGGGCCACAATTGCACTATTACTTCTTTCAAACTACCTCTTTCAAACATGGCAATTCAACAAGAAGAGCAAGGGCAAGAAATTACCTCCTGGCCCAAAAGGGTTTCCTATTATTGGCAGCCTCAATTTGCTAAAAGAGTTTCCTCATAGAGATCTTCATCAATTGTCTCAAAAATATGGTCCCATAATGCACATGAAATTGGGACTTGTAAACACAATTGTTGTCTCTTCCCCAACAGCTGCTGAGCTTTTTCTCAAAACCCATGACCTTATTTTTGCAAGTAGGCCCCTCACTATGGTCTCAAAATTCCTGTCTTATGGCCAAAAGAATTTGGTTTTTGCTCAATATGGATCTTATTGGAGGAATATACGTAAAATGTGTACGCTTGAATTGCTTAGTAACCACAAGATCAATTCTTTCAAATCCATGAGAAAGGAAGAACTTGGATTGTTGATTGAGCATCTCAAAGAGGCTGCAAATGATGGTGTTGCTATTAATCTTAGTTCTAAAGTCGTGTCACTCAGCACAGATATGACTTGTTTGATGGTTTTTGGGAGGAAATTTGGAGACGAAGAGTTGGATGATAGAGGTTTTAAGGCTATGATGCAAGAGGTGATGCAATTAATCGCTGCTCCTAATTTGGGAGATTTTATTCCTTTTATTGCAAGACTTGATCTCCAAGGGTTGACACGTCGGATGAAAAATGTTAGCAAAGTGTTTGATGGGTTCTTTGAGAGGATTATTGATGAACATCTTAACTCTATGGGTGGAAAAAGAACTAAAGATTTTTTAGATGTCATGTTGGATCTAATGGACTCTGTGGATACTCATGAGTATCGAATTGATCGTTCCTGCGTCAAAGCAATAATAATGGATATGCTTGGTGCTGCAATGGACACTGCAGCAACAGTAATTGGATGGGCACTTCCTGAGCTCATAAGACATCCACATGTAATGAAAAAATTGCAAGATGAAATAGAAAATGTGGTGGGGTTGGATAGAATGGTGCAAGAATCTGATTTAGTTCACTTGGAATATTTGGACATGGTAATGAAGGAAATCCTTAGGCTGTATCCACCAGCTCCATTGTTGGTTCCACATGAATCTATTGAAGAATGCATTGTTGAAGACTTTTTCATTCCAAAAAAATCACGTATAATAGTGAATGCATGGGCAATTGGACGGAACCCAAGTGCTTGGATTGATCCAGAAATGTTCTATCCAGAGAGATTTATTGGTAGCAAAGTGGATGTAAAGGGAAGAGATTTTCAATTAATTCCATTTGGGTTTGACTGTGGTTAG

Protein sequence

MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLSTDMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNVSKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLGAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGSKVDVKGRDFQLIPFGFDCG
Homology
BLAST of Bhi03G000955 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 322.4 bits (825), Expect = 5.6e-88
Identity = 162/415 (39.04%), Postives = 264/415 (63.61%), Query Frame = 0

Query: 23  FNKKSKGKK--LPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKYGPIMHMKLGLVNTIVVS 82
           F K+ +GKK    P P G P+IG+L+ L   PHR L  LS +YGP+M +  G V  +VVS
Sbjct: 20  FRKQKRGKKRNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSHRYGPLMLLHFGRVPVLVVS 79

Query: 83  SPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNIRKMCTLELLSNHK 142
           S   A   LKTHD +FASRP + + + L Y + ++  A YG YWR ++ +C L L SN  
Sbjct: 80  SAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMKSVCVLHLFSNKM 139

Query: 143 INSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLSTDMTCLMVFGRKFGDEELDDR 202
           + SF+ +R+EE+ L++E ++++ +  + +NLS  +VSL+ D+ C +  GRK+G E     
Sbjct: 140 VRSFREVREEEISLMMEKIRKSIS--LPVNLSKILVSLTNDVICKVALGRKYGGE----T 199

Query: 203 GFKAMMQEVMQLIAAPNLGDFIPFIARLD-LQGLTRRMKNVSKVFDGFFERIIDEHLNSM 262
            FK +M+ + +L+   ++G ++P++A +D ++GL  +++  +   D FFER++ +H++  
Sbjct: 200 DFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFERVVQDHVD-- 259

Query: 263 GGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLGAAMDTAATVIGWALPELIRH 322
           G +   DF+DV+L +     T  + I+R  +KAI+M++     DT++T++ WA+ EL+RH
Sbjct: 260 GNRDMTDFVDVLLAIQRD-KTVGFEINRVSIKAIVMNVFVGGTDTSSTLMEWAMTELLRH 319

Query: 323 PHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILRLYPPAPLLVPHESIEECIV 382
           P  +K+LQ+E+  +      V E ++ ++ YL  V+KE LRL+PP PL+VPHES ++  +
Sbjct: 320 PKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEALRLHPPLPLMVPHESTQDVRL 379

Query: 383 EDFFIPKKSRIIVNAWAIGRNPSAW-IDPEMFYPERFIGSKVDVKGRDFQLIPFG 434
            D  IP  +++++NAWAIGR  + W  D E F PER + S VD +G+ F+LIPFG
Sbjct: 380 GDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLDSSVDYRGQAFELIPFG 425

BLAST of Bhi03G000955 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 322.4 bits (825), Expect = 5.6e-88
Identity = 169/439 (38.50%), Postives = 269/439 (61.28%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           M  +W    +LL+S  L      + K +  + PP P G PIIG+L+ L E PH+ L +LS
Sbjct: 1   MTVLWFVSLILLISILLVAV--KHSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCKLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           +KYGP+M +KLG V T++VS+P  A+  LK +DL   SRP    ++ LSY   ++ F+++
Sbjct: 61  KKYGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRF 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
             YW+ +RK+C  EL  N +INS + +++ E+  LI+ + E+A+    +NLS   +SL+ 
Sbjct: 121 DDYWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNV 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLD--LQGLTRRMK 240
           ++ C  VFG  F    L++  F+ ++ E ++++ + +  DF P++  +     GL  R +
Sbjct: 181 NVICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRE 240

Query: 241 NVSKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEY-RIDRSCVKAIIMDM 300
              +  D F+E++ID HL     +   DF+D++L L        Y ++ R+ +KAI+M++
Sbjct: 241 RSVRDLDAFYEQMIDLHLQKNREESEDDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMNI 300

Query: 301 LGAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLD---RMVQESDLVHLEYLDMV 360
           L   ++T+A  + WA+ ELIR+P VMKK+Q EI   +G +   R++   ++ HL YL+MV
Sbjct: 301 LLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNMV 360

Query: 361 MKEILRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPER 420
           +KE  RL+P APLLVP E I E  +  + I  K+R+ VN WAIGR+P  W DPE F PER
Sbjct: 361 IKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPER 420

Query: 421 FIGSKVDVKGRDFQLIPFG 434
           F+   +DVKG+D++L+PFG
Sbjct: 421 FMDCDIDVKGQDYELLPFG 437

BLAST of Bhi03G000955 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.6e-87
Identity = 180/444 (40.54%), Postives = 264/444 (59.46%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNK-KSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQL 60
           MA ++ TI L  +   + + +   + +S   +LPPGP  +PIIG+L  +   PHR L  +
Sbjct: 1   MATLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAM 60

Query: 61  SQKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQ 120
              YGPI+H++LG V+ +V +S + AE FLK HD  FASRP    +K ++Y  ++LVFA 
Sbjct: 61  VTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAP 120

Query: 121 YGSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHL----KEAANDGVAINLSSKV 180
           YG  WR +RK+ ++ L S   +  FK +R+EE+G L   L     +  N G  +N+   V
Sbjct: 121 YGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNM-CVV 180

Query: 181 VSLSTDMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTR 240
            +L  +M    +FG    D + D+  F++M+ E+M L    N+GDF+P +  LDLQG+  
Sbjct: 181 NALGREMIGRRLFGAD-ADHKADE--FRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAG 240

Query: 241 RMKNVSKVFDGFFERIIDEH-LNSMGGKRTKDFLDVMLDLMDS-VDTHEYRIDRSCVKAI 300
           +MK + K FD F   I+ EH +N    K T D L  ++ L  + +D     +  + +KA+
Sbjct: 241 KMKRLHKRFDAFLSSILKEHEMNGQDQKHT-DMLSTLISLKGTDLDGDGGSLTDTEIKAL 300

Query: 301 IMDMLGAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDM 360
           +++M  A  DT+A+ + WA+ ELIRHP +M K Q+E++ VVG DR V ESD+  L YL  
Sbjct: 301 LLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQA 360

Query: 361 VMKEILRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPE 420
           V+KE  RL+PP PL +PH + E C +  + IPK S ++ N WAI R+P  W DP  F PE
Sbjct: 361 VIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPE 420

Query: 421 RFI----GSKVDVKGRDFQLIPFG 434
           RF+     S VDVKG DF+LIPFG
Sbjct: 421 RFLPGGEKSGVDVKGSDFELIPFG 439

BLAST of Bhi03G000955 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 319.7 bits (818), Expect = 3.6e-87
Identity = 167/429 (38.93%), Postives = 270/429 (62.94%), Query Frame = 0

Query: 10  LLLLSNYLFQTWQFNKKSK-GKK--LPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKYGPI 69
           ++L S  +F T  F KK K GKK   P  P   P+IG+L+ L   PHR L  LS +YGP+
Sbjct: 7   IILQSLIIFITILFFKKQKRGKKSNTPRSPPRLPLIGNLHQLGHHPHRSLCSLSHRYGPL 66

Query: 70  MHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQYGSYWRN 129
           M + LG V  +VVSS   A   LKTHD +FASRP + + + L Y  +++ FA YG YWR 
Sbjct: 67  MLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEYWRQ 126

Query: 130 IRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLSTDMTCLM 189
           I+ +C L LLSN  + SF+++R+EE+ L++E ++++++  + +N+S  + SL+ D+   +
Sbjct: 127 IKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSS--LQVNVSELLGSLTNDVISRI 186

Query: 190 VFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLD-LQGLTRRMKNVSKVFD 249
             GRK+  E       K +M+ +M L+   ++G ++P++  +D + GL  ++       D
Sbjct: 187 ALGRKYSGE----TDSKELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLD 246

Query: 250 GFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLGAAMDTA 309
            F E+++ +H++   G+RT DF+DV+L +     +  + IDR C+KAI++D+L A  D++
Sbjct: 247 EFLEKVVQDHVDG-DGQRT-DFVDVLLRIQRE-KSIGFEIDRLCIKAIVLDVLVAGTDSS 306

Query: 310 ATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILRLYPPA 369
             ++ WA+ EL+RHP  ++ LQ+E+  +   +  V E D+ ++ YL  V+KE  RL+PP 
Sbjct: 307 YALMDWAMTELLRHPECLRTLQEEVRTICKGNLSVSEEDIQNMSYLKAVIKETTRLHPPL 366

Query: 370 PLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAW-IDPEMFYPERFIGSKVDVKG 429
           PLL PHESI++ I+ D+ IP  +++++NAWAIGR  + W  D E F PER + S VD +G
Sbjct: 367 PLLAPHESIQDVILGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLDSSVDFRG 426

Query: 430 RDFQLIPFG 434
            +F+L+PFG
Sbjct: 427 HNFELVPFG 426

BLAST of Bhi03G000955 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 318.9 bits (816), Expect = 6.2e-87
Identity = 176/434 (40.55%), Postives = 265/434 (61.06%), Query Frame = 0

Query: 8   IALLLLSNYLFQTWQFNKKSKGK--KLPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKYGP 67
           +  LLL   L  T  F +KS+    KLPPGP   PIIG+L+ L   PH+ L  L + +GP
Sbjct: 5   LCFLLLLLLLLVTIIFTRKSQSSKLKLPPGPPKLPIIGNLHYLNGLPHKCLLNLWKIHGP 64

Query: 68  IMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQYGSYWR 127
           +M ++LG V  +V+SS  AAE  LKTHDL   SRP T+ SK +SY  K++ FA YG  WR
Sbjct: 65  VMQLQLGYVPLVVISSNQAAEEVLKTHDLDCCSRPETIASKTISYNFKDIGFAPYGEEWR 124

Query: 128 NIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLSTDMTCL 187
            +RK+  +EL S  K NSF+ +R+EE  LL++ L EA+     +NL   + +LS  + C 
Sbjct: 125 ALRKLAVIELFSLKKFNSFRYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSASIVCR 184

Query: 188 MVFGRKFGDEE-LDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLD-LQGLTRRMKNVSKV 247
           + FG+   + E +D+   + +     ++ A     +F P    LD + G ++ +  +   
Sbjct: 185 LAFGQNLHESEFIDEDSMEDLASRSEKIQAKFAFSNFFPGGWILDKITGQSKSLNEIFAD 244

Query: 248 FDGFFERIIDEHLNSMGGK--RTKDFLDVMLDLMDSVDTH-EYRIDRSCVKAIIMDMLGA 307
            DGFF +++D+HL   G K   T D +DVM+D+M+       +++    +K II D+  A
Sbjct: 245 LDGFFNQVLDDHLKP-GRKVLETPDVVDVMIDMMNKQSQDGSFKLTTDHIKGIISDIFLA 304

Query: 308 AMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDR-MVQESDLVHLEYLDMVMKEIL 367
            ++T+AT I WA+ ELIR+P VMKK+QDE+  V+G  R  + E DL  L Y  +V+KE  
Sbjct: 305 GVNTSATTILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLNYFKLVIKETF 364

Query: 368 RLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGSK 427
           RL+P APLL+P E++ +  ++ + IP+K++I+VN +AIGR+P  W +PE F PERF+ S 
Sbjct: 365 RLHPAAPLLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEEFKPERFVDSS 424

Query: 428 VDVKGRDFQLIPFG 434
           VD +G +F+L+PFG
Sbjct: 425 VDYRGLNFELLPFG 437

BLAST of Bhi03G000955 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 4.9e-161
Identity = 277/434 (63.82%), Postives = 353/434 (81.34%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           M WIWATI LL L  ++ Q W  NKK   K+LPPGP+GFPI GSL+LL EFP++DLH+L+
Sbjct: 1   MVWIWATIGLLALV-HILQAWWKNKK---KRLPPGPRGFPIFGSLHLLGEFPNKDLHRLA 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           +KYG IM+M+LGL+ TIV+SSP AAELFLKTHDL+FASRP    SK +S+GQKNL+F++Y
Sbjct: 61  RKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAAND-GVAINLSSKVVSLS 180
           G+YWR+ RKMCT+ELLSNHKINSFKSMR+EE+ L +E ++ AAN+ GVA++LS KV SLS
Sbjct: 121 GAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLS 180

Query: 181 TDMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKN 240
            DM+C MV G+K+ DEE D+RGFK++++E +QL +APNLGD+I FIA LDLQG T+RMK+
Sbjct: 181 VDMSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKS 240

Query: 241 VSKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLG 300
           V+K FD  FE+II+EHL    G+RT DF+DVM+  M S ++ EYRI+R  +KAI++DML 
Sbjct: 241 VNKAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEES-EYRIERPHIKAIMLDMLV 300

Query: 301 AAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEIL 360
           A+MDT+AT I WAL EL+RHP  MKK+Q E+ENVVGLD+MV+ESDL  L+YL+MV+KE  
Sbjct: 301 ASMDTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETF 360

Query: 361 RLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGSK 420
           RL+P APLL+PH SIE+C V  + IPKKSR+++N WAIGR+P+AW D E F PERF GS 
Sbjct: 361 RLHPVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSS 420

Query: 421 VDVKGRDFQLIPFG 434
           VDV+G  FQLIPFG
Sbjct: 421 VDVRGNHFQLIPFG 429

BLAST of Bhi03G000955 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.3e-117
Identity = 221/410 (53.90%), Postives = 290/410 (70.73%), Query Frame = 0

Query: 31  KLPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKYGPIMHMKLGLVNTIVVSSPTAAELFLK 90
           KLPPGP+G PIIGSL+ L   PHR L  L++KYGPIM M+LG V TIVVSSP AAELFLK
Sbjct: 32  KLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIMSMRLGSVPTIVVSSPQAAELFLK 91

Query: 91  THDLIFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNIRKMCTLELLSNHKINSFKSMRKE 150
           THD IFASRP    ++++SYG   + F  YG +WRNIRK   LELL+  KINSF  MR+E
Sbjct: 92  THDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNIRKFVVLELLTPAKINSFVGMRRE 151

Query: 151 ELGLLIEHLKEAANDGVAINLSSKVVSLSTDMTCLMVFGRKFGDEELDDR-GFKAMMQEV 210
           ELG +++ +KEA+     ++LS+KV ++  +MT  ++ GR       DDR   K +M E 
Sbjct: 152 ELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLLLGR-----TKDDRYDLKGIMNEA 211

Query: 211 MQLIAAPNLGDFIPFIARLDLQGLTRRMKNVSKVFDGFFERIIDEH-LNSMGGKRTKDFL 270
           + L    N+ DF+PF+  LD+QGLTR+ K+  K  D   E IIDEH  NS  G  + DF+
Sbjct: 212 LTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASGDFI 271

Query: 271 DVMLDLMDS-VDTHEYR---IDRSCVKAIIMDMLGAAMDTAATVIGWALPELIRHPHVMK 330
           D ML L +   +TH+     IDRS +KAI++D++ AA+DT+ T I W L ELI+HP  MK
Sbjct: 272 DDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHPRAMK 331

Query: 331 KLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILRLYPPAPLLVPHESIEECIVEDFFI 390
           K Q+EI+ VVG+DRMV+E+DL +LEY+ MV+KE LRL+P APLL PHES+E+  +  +FI
Sbjct: 332 KCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRLHPVAPLLGPHESMEDITINGYFI 391

Query: 391 PKKSRIIVNAWAIGRNPSAWID-PEMFYPERFIGSKVDVKGRDFQLIPFG 434
           PK+SR+IVN+WA+GR+P+ W +  E F PERF GS VDV+GRDFQL+PFG
Sbjct: 392 PKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNVDVRGRDFQLLPFG 436

BLAST of Bhi03G000955 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 5.6e-101
Identity = 188/435 (43.22%), Postives = 281/435 (64.60%), Query Frame = 0

Query: 16  YLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKYGPIMHMKLGLVN 75
           + F  W  ++  + ++LPPGP  +PIIG+ + ++   HR L  L++KYGPI+ ++ G V 
Sbjct: 26  FFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILFLRFGSVP 85

Query: 76  TIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNIRKMCTLEL 135
           T+VVSS   A+ FLKTHDLIFASRP T V K+  Y  K++ F+ YG +WR +RK+C LEL
Sbjct: 86  TVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLEL 145

Query: 136 LSNHKINSFKSMRKEELGLLIEHLKEAANDG-VAINLSSKVVSLSTDMTCLMVFGRKFGD 195
           L++ +I SFK +R+EEL  +I  + E +  G +A+N+S  + +   ++   ++  +KF D
Sbjct: 146 LTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRILARKKFSD 205

Query: 196 EEL--DDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNVSKVFDGFFERII 255
            +L  D +GF  ++ EV   + + N+GDFIP++  LDLQG+ R +K  +  FD F E++I
Sbjct: 206 NDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFDAFAEKMI 265

Query: 256 DEHLNSM--------GGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLGAAMDT 315
           DEH+N+          G   KD +DV+L++  + D    ++ R  +KAI  ++  A M+T
Sbjct: 266 DEHINASTIRNGEADAGCHVKDIIDVLLEMAKN-DNTGAKVTREIIKAITYELFSAGMET 325

Query: 316 AATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILRLYPP 375
           +A V+ WA+ EL+RHPH MKKLQ EIE+VVG    V+ESDL  + YL  V+KE LRLYP 
Sbjct: 326 SANVLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKETLRLYPS 385

Query: 376 APLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAW-IDPEMFYPERFIGSK---V 434
            PL +PHES+E   V  ++IPKK+ +I+N WAIGR+PS W  D   F PERF+  +   +
Sbjct: 386 LPLALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERFMQMEENGI 445

BLAST of Bhi03G000955 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 359.4 bits (921), Expect = 5.8e-98
Identity = 180/417 (43.17%), Postives = 263/417 (63.07%), Query Frame = 0

Query: 25  KKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKYGPIMHMKLGLVNTIVVSSPTA 84
           K+ K   LPP P   PIIG+L+ L   PHR L  L+ + GP++ + LG + T++VS+   
Sbjct: 24  KREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILLHLGHIPTLIVSTAEI 83

Query: 85  AELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNIRKMCTLELLSNHKINSF 144
           AE  LKTHDLIFASRP T  ++ + Y   ++ F+ YG YWR +RK+C LELLS  ++NS+
Sbjct: 84  AEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNSY 143

Query: 145 KSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLSTDMTCLMVFGRKFGDEELDDRGFKA 204
           +S+R+EE+GL++E + ++ + G A+NLS  ++ LS+     + FG+K+  EE     F  
Sbjct: 144 RSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEEERKNKFAD 203

Query: 205 MMQEVMQLIAAPNLGDFIPFIARLD-LQGLTRRMKNVSKVFDGFFERIIDEHL-----NS 264
           +  E+  L+ A  +GD+ P  A +D L G+  R+K      D F + +ID+HL     N 
Sbjct: 204 LATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRKANG 263

Query: 265 MGGKRTKDFLDVMLDLM--DSVDTHEYRIDRSCVKAIIMDMLGAAMDTAATVIGWALPEL 324
             G   KD +DV+L L    S+  H   ++R+ +KA+I+DM     DT A  + WA+ EL
Sbjct: 264 SDGVEQKDLVDVLLHLQKDSSLGVH---LNRNNLKAVILDMFSGGTDTTAVTLEWAMAEL 323

Query: 325 IRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILRLYPPAPLLVPHESIEE 384
           I+HP VM+K Q E+  VVG    V+E DL  L YL +++KE LRL+P APLLVP ES  +
Sbjct: 324 IKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLHPVAPLLVPRESTRD 383

Query: 385 CIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGSKVDVKGRDFQLIPFG 434
            ++  + IP K+R+ +NAWAIGR+P +W + E F PERF+ + VD KG+DFQLIPFG
Sbjct: 384 VVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSVDFKGQDFQLIPFG 437

BLAST of Bhi03G000955 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.2e-92
Identity = 179/433 (41.34%), Postives = 271/433 (62.59%), Query Frame = 0

Query: 4   IWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLSQKY 63
           ++A   L+LL  ++++      + +  KLPP P   P+IG+L+ L   PH  L  L++KY
Sbjct: 23  LFAFSLLILLLKFIYKD---KSRKRRVKLPPSPPKLPVIGNLHQLGNKPHLSLRCLAEKY 82

Query: 64  GPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQYGSY 123
           GPI++++LG + T+VVSS   A+  LKTHDL  +SRP    +K L Y   ++VF+ YG+Y
Sbjct: 83  GPIIYLQLGEIPTVVVSSARLAKEVLKTHDLALSSRPQIFSAKHLFYNCTDVVFSPYGAY 142

Query: 124 WRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLSTDMT 183
           WR+IRK+C LELLS  ++ SF  +R+EE+  L+  + E        NL+  +   + D+ 
Sbjct: 143 WRHIRKICILELLSAKRVQSFSHVREEEVARLVRRVAEFYPG--TTNLTKMLGLYANDVL 202

Query: 184 CLMVFGRKFGD-EELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARL-DLQGLTRRMKNVS 243
           C + FGR F +  + D  GF+ M++E  +L+   ++GDF P +  +  L G+  R++   
Sbjct: 203 CRVAFGRGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETF 262

Query: 244 KVFDGFFERIIDEHLNSMGGKRT-KDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLGA 303
           + FD  F++++ +HL+    K   KD +DV+LD+     T E  +    VKAII+DM  A
Sbjct: 263 RRFDELFDQMVTDHLSPKREKEEHKDLVDVLLDIQKKEST-EMPLTMDNVKAIILDMFAA 322

Query: 304 AMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILR 363
             DT    + W + EL+ +  V+++ Q E+  VVG  R+V ESDL  L+Y+  V+KEI R
Sbjct: 323 GTDTTFITLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFR 382

Query: 364 LYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGSKV 423
           L+PPAP+LVP ES+E+  ++ + I  K+RI VNAWAIGR+P +W DPE F PERFIGS +
Sbjct: 383 LHPPAPVLVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTI 442

Query: 424 DVKGRDFQLIPFG 434
           D KG+DF+LIPFG
Sbjct: 443 DFKGQDFELIPFG 449

BLAST of Bhi03G000955 vs. NCBI nr
Match: XP_038883676.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 840.9 bits (2171), Expect = 5.0e-240
Identity = 411/433 (94.92%), Postives = 422/433 (97.46%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS
Sbjct: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY
Sbjct: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
           GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST
Sbjct: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNV 240
           DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLT RMKNV
Sbjct: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTCRMKNV 240

Query: 241 SKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLGA 300
           SKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMD+LGA
Sbjct: 241 SKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDILGA 300

Query: 301 AMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILR 360
           AMDT+ATVIGWALPELIRHP  MKK+QDE+ENVVGLDR+VQESDL+HLEYLDMV+KE+LR
Sbjct: 301 AMDTSATVIGWALPELIRHPQAMKKMQDELENVVGLDRIVQESDLIHLEYLDMVIKEVLR 360

Query: 361 LYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGSKV 420
           LYPP PLLVPHES+E+CIV DFFIPKKSRIIVN W IGRNPS WI PE FYPERFIG KV
Sbjct: 361 LYPPVPLLVPHESLEDCIVNDFFIPKKSRIIVNTWTIGRNPSTWIHPEKFYPERFIGGKV 420

Query: 421 DVKGRDFQLIPFG 434
           DV+GRDFQLIPFG
Sbjct: 421 DVRGRDFQLIPFG 433

BLAST of Bhi03G000955 vs. NCBI nr
Match: XP_004143494.1 (cytochrome P450 CYP736A12 [Cucumis sativus])

HSP 1 Score: 775.4 bits (2001), Expect = 2.6e-220
Identity = 377/435 (86.67%), Postives = 408/435 (93.79%), Query Frame = 0

Query: 1   MAWIWATI--ALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQ 60
           MAWIWATI   L LLSNY+F+TW F KKSKGKKLPPGPKGFPI GSLNLLKEFPHRDLH+
Sbjct: 1   MAWIWATIIALLFLLSNYIFETWLFKKKSKGKKLPPGPKGFPIFGSLNLLKEFPHRDLHR 60

Query: 61  LSQKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFA 120
           LSQKYGPIMH+KLGLVNTI+VSSP AAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKYGPIMHIKLGLVNTIIVSSPPAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSL 180
           QYGSYWRN+RKMCTLELLSNHKINSFKSMRKEE+GLLIE+LKEAANDGV++N+SSKVVSL
Sbjct: 121 QYGSYWRNVRKMCTLELLSNHKINSFKSMRKEEVGLLIEYLKEAANDGVSVNISSKVVSL 180

Query: 181 STDMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMK 240
           STDMTCLMVFGRKFGDEELDDRGFKAM+QEVMQL+AAPNLGD IPFIA  DLQGLTRRMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDLIPFIAMFDLQGLTRRMK 240

Query: 241 NVSKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDML 300
           N++KVFD FFERIIDEHL SMG K+TKDFLDVMLDLM S DTHEYRIDRS VKAII+D+L
Sbjct: 241 NINKVFDRFFERIIDEHLKSMGEKKTKDFLDVMLDLMKSEDTHEYRIDRSSVKAIILDVL 300

Query: 301 GAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEI 360
           GAAMDT+ATVIGWAL ELIRHP VMKK+QDE+ENVVGLDRMV+E DL+HLEYLDMV+KEI
Sbjct: 301 GAAMDTSATVIGWALAELIRHPDVMKKVQDEVENVVGLDRMVEELDLIHLEYLDMVIKEI 360

Query: 361 LRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGS 420
           LRLYPP PLLVPHES+++CIVEDFFIPK SR+IVNAWAIGRNPSAWI P  F PERF+GS
Sbjct: 361 LRLYPPLPLLVPHESLQDCIVEDFFIPKNSRVIVNAWAIGRNPSAWIHPLKFCPERFMGS 420

Query: 421 KVDVKGRDFQLIPFG 434
            +DV+GRDFQLIPFG
Sbjct: 421 HLDVRGRDFQLIPFG 435

BLAST of Bhi03G000955 vs. NCBI nr
Match: XP_022950766.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 774.2 bits (1998), Expect = 5.8e-220
Identity = 379/435 (87.13%), Postives = 413/435 (94.94%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           M WIWAT+ALLLLS+YLFQTW+FN KSKGKKLPPGPKGFPIIGSLNLLK+FPHRDLH+LS
Sbjct: 1   MPWIWATLALLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           QK+GPIMHMKLGLVNTIVVSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFAQY
Sbjct: 61  QKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
           GSYWRN+RKMCTLELLS  KINSFKSMRKEE+GL+IE LK+AANDGVA++LSSKVVSLST
Sbjct: 121 GSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLST 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNV 240
           DMTCLMVFGRKFGDEELDDRGFKAM+QEVMQL+AAPNLGDFIPFIA  DLQGLTRRMKNV
Sbjct: 181 DMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFIATFDLQGLTRRMKNV 240

Query: 241 SKVFDGFFERIIDEHL--NSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDML 300
           S+VFDGFFERIIDEHL   S+G KRTKDFLDVMLDL+ S DT EYR+DRS VKAII+D+L
Sbjct: 241 SQVFDGFFERIIDEHLMSKSLGEKRTKDFLDVMLDLIGSEDT-EYRVDRSSVKAIILDIL 300

Query: 301 GAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEI 360
           GAAMDT+ATVIGWALPELIRHP VMKK+QDE+ENVVGLDRMV+ESDL+HLEYLDMV KEI
Sbjct: 301 GAAMDTSATVIGWALPELIRHPDVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVTKEI 360

Query: 361 LRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGS 420
           LRLYPP PLLVPHES+++CIV+DFFIPKKSR+IVNAWAIGRNPSAW +PE FYPERF GS
Sbjct: 361 LRLYPPVPLLVPHESLQDCIVDDFFIPKKSRVIVNAWAIGRNPSAWANPEKFYPERFTGS 420

Query: 421 KVDVKGRDFQLIPFG 434
           +VDV+GRDFQLIPFG
Sbjct: 421 QVDVRGRDFQLIPFG 434

BLAST of Bhi03G000955 vs. NCBI nr
Match: XP_023543390.1 (cytochrome P450 CYP736A12-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.5 bits (1970), Expect = 1.0e-216
Identity = 373/435 (85.75%), Postives = 411/435 (94.48%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           M WIWAT+ALLLLS+YLFQTW+FN KSKGKKLPPGPKGFPIIGSLNLLK+FPHRDLH+LS
Sbjct: 1   MPWIWATLALLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           QK+GPIMHMKLGLVNTIVVSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFAQY
Sbjct: 61  QKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
           GSYWRN+RKMCTLELLS  KINSFKSMRKEE+GL+IE LK+AANDGVA++LSSKVVSLST
Sbjct: 121 GSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLST 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNV 240
           DMTCLMVFGRKFGDEELDDRGFKAM+QEVMQL+AAPNLGDFIPFI+  DLQGLTRRMKNV
Sbjct: 181 DMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFISTFDLQGLTRRMKNV 240

Query: 241 SKVFDGFFERIIDEHL--NSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDML 300
           S+VFDGFFERIIDEHL   S G KRTKDFLDVMLDL+ S DT EYRIDRS VKAII+D+L
Sbjct: 241 SQVFDGFFERIIDEHLKSKSFGEKRTKDFLDVMLDLIGSEDT-EYRIDRSSVKAIILDIL 300

Query: 301 GAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEI 360
           GAAMDT+ATVIGWALPELIRHP VMK++QDE+E+VVGLDRMV+ESDL+HLEYLDMV+KEI
Sbjct: 301 GAAMDTSATVIGWALPELIRHPDVMKRVQDELEDVVGLDRMVEESDLIHLEYLDMVIKEI 360

Query: 361 LRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGS 420
           LRLYPP PLLVPHES+++CIV DFFIPKKSR+IVNAWAIGRNP+ W +P+ FYPERF GS
Sbjct: 361 LRLYPPVPLLVPHESLQDCIVGDFFIPKKSRVIVNAWAIGRNPNVWANPKKFYPERFNGS 420

Query: 421 KVDVKGRDFQLIPFG 434
           ++DV+GRDFQLIPFG
Sbjct: 421 QIDVRGRDFQLIPFG 434

BLAST of Bhi03G000955 vs. NCBI nr
Match: XP_023543389.1 (cytochrome P450 CYP736A12-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.5 bits (1970), Expect = 1.0e-216
Identity = 373/435 (85.75%), Postives = 411/435 (94.48%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           M WIWAT+ALLLLS+YLFQTW+FN KSKGKKLPPGPKGFPIIGSLNLLK+FPHRDLH+LS
Sbjct: 1   MPWIWATLALLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           QK+GPIMHMKLGLVNTIVVSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFAQY
Sbjct: 61  QKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
           GSYWRN+RKMCTLELLS  KINSFKSMRKEE+GL+IE LK+AANDGVA++LSSKVVSLST
Sbjct: 121 GSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLST 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNV 240
           DMTCLMVFGRKFGDEELDDRGFKAM+QEVMQL+AAPNLGDFIPFI+  DLQGLTRRMKNV
Sbjct: 181 DMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFISTFDLQGLTRRMKNV 240

Query: 241 SKVFDGFFERIIDEHL--NSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDML 300
           S+VFDGFFERIIDEHL   S G KRTKDFLDVMLDL+ S DT EYRIDRS VKAII+D+L
Sbjct: 241 SQVFDGFFERIIDEHLKSKSFGEKRTKDFLDVMLDLIGSEDT-EYRIDRSSVKAIILDIL 300

Query: 301 GAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEI 360
           GAAMDT+ATVIGWALPELIRHP VMK++QDE+E+VVGLDRMV+ESDL+HLEYLDMV+KEI
Sbjct: 301 GAAMDTSATVIGWALPELIRHPDVMKRVQDELEDVVGLDRMVEESDLIHLEYLDMVIKEI 360

Query: 361 LRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGS 420
           LRLYPP PLLVPHES+++CIV DFFIPKKSR+IVNAWAIGRNP+ W +P+ FYPERF GS
Sbjct: 361 LRLYPPVPLLVPHESLQDCIVGDFFIPKKSRVIVNAWAIGRNPNVWANPKKFYPERFNGS 420

Query: 421 KVDVKGRDFQLIPFG 434
           ++DV+GRDFQLIPFG
Sbjct: 421 QIDVRGRDFQLIPFG 434

BLAST of Bhi03G000955 vs. ExPASy TrEMBL
Match: A0A6J1GFR1 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453773 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.8e-220
Identity = 379/435 (87.13%), Postives = 413/435 (94.94%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           M WIWAT+ALLLLS+YLFQTW+FN KSKGKKLPPGPKGFPIIGSLNLLK+FPHRDLH+LS
Sbjct: 1   MPWIWATLALLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           QK+GPIMHMKLGLVNTIVVSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFAQY
Sbjct: 61  QKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
           GSYWRN+RKMCTLELLS  KINSFKSMRKEE+GL+IE LK+AANDGVA++LSSKVVSLST
Sbjct: 121 GSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSLST 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNV 240
           DMTCLMVFGRKFGDEELDDRGFKAM+QEVMQL+AAPNLGDFIPFIA  DLQGLTRRMKNV
Sbjct: 181 DMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFIATFDLQGLTRRMKNV 240

Query: 241 SKVFDGFFERIIDEHL--NSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDML 300
           S+VFDGFFERIIDEHL   S+G KRTKDFLDVMLDL+ S DT EYR+DRS VKAII+D+L
Sbjct: 241 SQVFDGFFERIIDEHLMSKSLGEKRTKDFLDVMLDLIGSEDT-EYRVDRSSVKAIILDIL 300

Query: 301 GAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEI 360
           GAAMDT+ATVIGWALPELIRHP VMKK+QDE+ENVVGLDRMV+ESDL+HLEYLDMV KEI
Sbjct: 301 GAAMDTSATVIGWALPELIRHPDVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVTKEI 360

Query: 361 LRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGS 420
           LRLYPP PLLVPHES+++CIV+DFFIPKKSR+IVNAWAIGRNPSAW +PE FYPERF GS
Sbjct: 361 LRLYPPVPLLVPHESLQDCIVDDFFIPKKSRVIVNAWAIGRNPSAWANPEKFYPERFTGS 420

Query: 421 KVDVKGRDFQLIPFG 434
           +VDV+GRDFQLIPFG
Sbjct: 421 QVDVRGRDFQLIPFG 434

BLAST of Bhi03G000955 vs. ExPASy TrEMBL
Match: A0A6J1IMS2 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.6e-215
Identity = 369/435 (84.83%), Postives = 410/435 (94.25%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           MAWIWAT+ALLLLS+YLFQTW+FN KSKGKKLPPGPKGFPIIGSLNLLK+FPHRDLH+LS
Sbjct: 1   MAWIWATLALLLLSHYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHRLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           QKYGPIMHMKLGLVNT VVSS  AAELFLKTHDL+FASRPLTMVSKFLSYGQKNL+FAQY
Sbjct: 61  QKYGPIMHMKLGLVNTTVVSSAEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLIFAQY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
           GSYWRN+RKMCTLELLS  KINSFKSMRKEE+GL+IE+LK+AANDGVA++LSSK+VSLST
Sbjct: 121 GSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIEYLKKAANDGVAVDLSSKIVSLST 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNV 240
           DMTCLMVFGRKFGDEELDDRGFKAM+QEVMQL+AAPNLGDFIPFIA  DLQGLTRRMK V
Sbjct: 181 DMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFIATFDLQGLTRRMKIV 240

Query: 241 SKVFDGFFERIIDEHL--NSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDML 300
           S+VFDGFFERIIDEHL   S+G KRTKDFLD MLDL+ S DT EYRIDRS VKAII+D+L
Sbjct: 241 SQVFDGFFERIIDEHLKSRSLGEKRTKDFLDFMLDLIGSEDT-EYRIDRSSVKAIILDIL 300

Query: 301 GAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEI 360
           GAAMDT+ATVIGWALPELIRHP VM K+QDE+E+VVGLDRMV+ESDL+HLEYLDMV+KEI
Sbjct: 301 GAAMDTSATVIGWALPELIRHPDVMMKVQDELEHVVGLDRMVEESDLIHLEYLDMVIKEI 360

Query: 361 LRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGS 420
           LRLYPP PLLVPHES+++CI++D+FIPKKSR+I+NAWAIGRNPS+W +PE FYPERF GS
Sbjct: 361 LRLYPPVPLLVPHESLQDCIIDDYFIPKKSRVILNAWAIGRNPSSWANPEKFYPERFNGS 420

Query: 421 KVDVKGRDFQLIPFG 434
           +VDV+GR FQLIPFG
Sbjct: 421 QVDVRGRHFQLIPFG 434

BLAST of Bhi03G000955 vs. ExPASy TrEMBL
Match: A0A6J1BWX1 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005474 PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 5.9e-202
Identity = 352/435 (80.92%), Postives = 391/435 (89.89%), Query Frame = 0

Query: 1   MAWIWATIA-LLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQL 60
           M WIWATIA LLLL+NYL Q W FN     KKLPPGPKGFPI+G LNLL +FPHRDL +L
Sbjct: 1   MPWIWATIALLLLLANYLLQPWLFN----SKKLPPGPKGFPILGCLNLLGKFPHRDLQKL 60

Query: 61  SQKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQ 120
           SQKYGPIMHM+LGLVNTIVVSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFAQ
Sbjct: 61  SQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQ 120

Query: 121 YGSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLS 180
           YGSYWRN+RKMCTLELLSN KINSFKSMRKEELGLLI+ ++E ANDG  ++LSS+V SLS
Sbjct: 121 YGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSLS 180

Query: 181 TDMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDF-IPFIARLDLQGLTRRMK 240
           TDMTCLMVFGRKF DEE DDRGFK M+QEVMQL+AAPNLGDF IPF+A  DLQGLTRRMK
Sbjct: 181 TDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVATFDLQGLTRRMK 240

Query: 241 NVSKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDML 300
           NVSKVFD FFERIIDEHL  MG KR+KDF+DVMLDLM S +T EY+IDRS +KAI++D+L
Sbjct: 241 NVSKVFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEET-EYQIDRSSIKAIMLDIL 300

Query: 301 GAAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEI 360
           GAAMDT+ATVIGWALPELI++P VMKK+QDE+E VVGLDRMV+ESDLV+LEYLDMV+KE 
Sbjct: 301 GAAMDTSATVIGWALPELIKNPQVMKKVQDELEKVVGLDRMVEESDLVNLEYLDMVIKET 360

Query: 361 LRLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGS 420
           LRLYPP PLLVPHES+E+C+V DF IPKKSR+IVNAWAIGRNPS W DPE FYPERF+ S
Sbjct: 361 LRLYPPVPLLVPHESLEDCVVNDFHIPKKSRVIVNAWAIGRNPSTWTDPEKFYPERFVDS 420

Query: 421 KVDVKGRDFQLIPFG 434
           +VDV+GRDFQLIPFG
Sbjct: 421 QVDVRGRDFQLIPFG 430

BLAST of Bhi03G000955 vs. ExPASy TrEMBL
Match: A0A6J1BVB2 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005855 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 4.2e-176
Identity = 309/435 (71.03%), Postives = 371/435 (85.29%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           MAWIW T+  L+    L ++  F   +K KKLPPGPKGFPI GSL+LL + PHRDL +LS
Sbjct: 1   MAWIWVTLVSLIA--LLLRSCLFKNHNKRKKLPPGPKGFPIFGSLHLLGKLPHRDLQKLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           Q+YGPIMHMKLGLV TI+VSSP+AAELFLKTHDL+FASRP +  SK +SY QKNLVF+ Y
Sbjct: 61  QEYGPIMHMKLGLVPTIIVSSPSAAELFLKTHDLVFASRPPSEASKHISYRQKNLVFSPY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAAN-DGVAINLSSKVVSLS 180
           G YWRN+RKMCTLELLSN KINSFKSMRK +LGLLI+HL+EAA  D VA+NLS KV S +
Sbjct: 121 GPYWRNMRKMCTLELLSNLKINSFKSMRKHQLGLLIQHLREAAACDRVAVNLSDKVTSFT 180

Query: 181 TDMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKN 240
           TD+TCLMVFG+K+ D+E D+RGFKA+MQ+ +QL AAPNLGDFIPFIA LDLQGLTR+MK 
Sbjct: 181 TDITCLMVFGKKYEDKEFDERGFKAVMQQGLQLGAAPNLGDFIPFIAGLDLQGLTRQMKA 240

Query: 241 VSKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLG 300
           V KVFDGF E+II+EHL +    +TKDF+DVMLDL+DS +  EY+IDRS +KAI++DML 
Sbjct: 241 VHKVFDGFLEKIINEHLEARDEHKTKDFVDVMLDLVDS-EQSEYQIDRSAIKAIMLDMLA 300

Query: 301 AAMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEIL 360
           AAMDT+AT IGWA+PELI+HPH+MKK+Q E+E VVGLDR+V+ESDL +LEYL+MV+KEIL
Sbjct: 301 AAMDTSATAIGWAMPELIKHPHIMKKMQVELEKVVGLDRVVEESDLENLEYLEMVVKEIL 360

Query: 361 RLYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAW-IDPEMFYPERFIGS 420
           RLYPPAPLL+P ES+E+CIV+ F IPKKSR+IVN WAIGR+PS W IDPE F+PERFI S
Sbjct: 361 RLYPPAPLLIPRESLEDCIVKGFHIPKKSRVIVNVWAIGRDPSGWTIDPEKFFPERFIDS 420

Query: 421 KVDVKGRDFQLIPFG 434
           ++DV+GRDFQLIPFG
Sbjct: 421 QIDVRGRDFQLIPFG 432

BLAST of Bhi03G000955 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.5e-176
Identity = 299/433 (69.05%), Postives = 367/433 (84.76%), Query Frame = 0

Query: 1   MAWIWATIALLLLSNYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQLS 60
           MAWI   +AL  L+  + + W F  ++   KLPPGP+GFP+ GSL+LL + PHRDL  LS
Sbjct: 1   MAWILLAVALAGLALLILKPWLFRSQN---KLPPGPRGFPVFGSLHLLGKLPHRDLRDLS 60

Query: 61  QKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFAQY 120
           QKYG IM+M+LG+V TI+V+SP AAELFLKTHDL+FASRP    SK++SYGQKNL FAQY
Sbjct: 61  QKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQY 120

Query: 121 GSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSLST 180
           GSYWRN+RKMCTLELLS+HK+NSF+SMR+EE+GLL+ +L++AA D V INLSSK+ SL+T
Sbjct: 121 GSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNT 180

Query: 181 DMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTRRMKNV 240
           DMTCLMVFG+K+ D E D+RGFK+++QE MQL+A PNLGDFIP IA LDLQGL RR K +
Sbjct: 181 DMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAI 240

Query: 241 SKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDMLGA 300
           SK+FDGFFE+IIDEHL S    +TKDF+DVMLD+M S +T EY+IDRS +KAII+DML A
Sbjct: 241 SKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQET-EYQIDRSSIKAIILDMLVA 300

Query: 301 AMDTAATVIGWALPELIRHPHVMKKLQDEIENVVGLDRMVQESDLVHLEYLDMVMKEILR 360
           AMDT+AT +GWA+ ELI+HP VMKKLQ+E++ VVGLDR V+ESDL HLEYLDM +KEILR
Sbjct: 301 AMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILR 360

Query: 361 LYPPAPLLVPHESIEECIVEDFFIPKKSRIIVNAWAIGRNPSAWIDPEMFYPERFIGSKV 420
           ++P APLLVPHES+E+C V  + IPKKSRIIVNAWAIG++P++W+DPE F+PERFIGS+V
Sbjct: 361 VHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQV 420

Query: 421 DVKGRDFQLIPFG 434
           DVKGRDFQLIPFG
Sbjct: 421 DVKGRDFQLIPFG 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G48270.15.6e-8839.04cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT5G57260.15.6e-8838.50cytochrome P450, family 71, subfamily B, polypeptide 10 [more]
AT5G07990.11.6e-8740.54Cytochrome P450 superfamily protein [more]
AT3G48320.13.6e-8738.93cytochrome P450, family 71, subfamily A, polypeptide 21 [more]
AT3G26210.16.2e-8740.55cytochrome P450, family 71, subfamily B, polypeptide 23 [more]
Match NameE-valueIdentityDescription
A0A068Q5V64.9e-16163.82Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH184.3e-11753.90Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q50EK45.6e-10143.22Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1[more]
P244655.8e-9843.17Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A068Q7212.2e-9241.34Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883676.15.0e-24094.92cytochrome P450 71AU50-like [Benincasa hispida][more]
XP_004143494.12.6e-22086.67cytochrome P450 CYP736A12 [Cucumis sativus][more]
XP_022950766.15.8e-22087.13cytochrome P450 CYP736A12-like [Cucurbita moschata][more]
XP_023543390.11.0e-21685.75cytochrome P450 CYP736A12-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023543389.11.0e-21685.75cytochrome P450 CYP736A12-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1GFR12.8e-22087.13cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453773 PE=... [more]
A0A6J1IMS21.6e-21584.83cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 ... [more]
A0A6J1BWX15.9e-20280.92cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005474 PE... [more]
A0A6J1BVB24.2e-17671.03cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005855 PE... [more]
A0A6J1BYU05.5e-17669.05cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 60..79
score: 31.51
coord: 393..417
score: 34.23
coord: 309..335
score: 37.83
coord: 178..196
score: 19.99
coord: 352..370
score: 36.17
coord: 289..306
score: 24.54
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 19..435
e-value: 1.0E-110
score: 372.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..434
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..434
e-value: 3.3E-87
score: 293.2
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 1..433
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 1..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000955Bhi03M000955mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen