Bhi03G000893 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000893
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Epidermal patterning factor-like protein
Location	chr3: 23917820 .. 23920481 (+)
RNA-Seq Expression	Bhi03G000893
Synteny	Bhi03G000893

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTACATGTAACACTTGCCCTATCAAAACCATACAATTTGGTGGCTACGGAAGGTAAGCAAGCTGGTGTTCAGACAGAGGAATGAAACAGCCAAGTTCGTCAGGTGCAGTTTATTGCTCTCTCTTTGCGTCTAAAGAATTTAAAAGCAGTGAAACACGATCAAAGAAAAAGAAGATCATTGAAGTCGTAATATTGCGAGAAATAGAGTAGGGTTTGGACTTTGGAGGGTTTTGATTTGTTCACAAGGATGCAGTCTTTATAATGGTCTGATCAATAGTCTGTAAAGTACATTGTGATTTTTACCGAGACTTAACAACTCCATGGCACTGGAACCAAGCAGTCTCAGGTACACTTTTCTCATACATGTTTTTGTATTTTCGTTGATTTATGGGAAAAAGAGACTGGTTAGGCTAAGAGAGTTCAGATGCAGAGAAAGTTTCCTTTTTTCTGCTCAGTGCTGACTGATTCACTGTTGAATTCCAAATGAACTCAGAAAACAGTTATCATATTTATGTTGATGAGAGGTTCATACGTTCAAGCTGAAAGGTAACAATAAATGTAAGGAAAAAATTGAAAATAAAATTCGCAAAACAAAAAAGGAGAACAAACAAAGAAAATTTAATTGTACATATGTAGAAAATACAATTCCCTTCCCCCCCCCGTGTGAATAAATAAATAAATCTTTATTCCTTCTCCGTGTGAATAAGGCTTTCAACTTTCGTGAAAGAGTCTTCTGAAGTTGGCCATCAGATCAGACTAGGGGTTCTTGAGGTTGAGTTTGCTCACTGCCGTCTCAGGCTCTTTTTCTTGGAGTTGGTAATGTTTCGGAATGATCTGATGCATGGTTGATAATTTAGTTGACTAATTGCCTTTTAAAAATAAATCAACAGATCTGGAGAGACAGTCGAGGAGTGTGGCAGAGTATTTGCAACATTTTTTTCGCCTTTAACGAGCAAACACTACATATTCTTTGTATTTAGGGCGACTTGATCTTCTAACTTGACACTTTTTTATTCATTTGGATCAACCATGGTCGCTTGAGTTCTGTAGAATTTTCAATATTTGATTATTTTTTCCAGTTGAATGTTGGTAGATTTCAATAGAGGCTTCCCCCCTCTTCATTTGGTAGTTGGTTCAAAGTTTTGAATCAAACCTAGCTAATCCACTTGCATTCTTAATTTAGATTTGCCAACTTGAGTATATTTAAATTAGTTTAGTCATATATGATCAAGTTATCGATCATAAAGTTAGAAGATTAAATCCTCGTCCTTACTTATTATTTTGAACAACCCCTCCCCCAAAAAATAGTGTCCTACTTTCCCAGTCATAAGTAATTGTTATATACATAGGATCACGAGAGATTTGAATTATAAATTGGAAGAAAAGCTAGCTTTGTAAATACTTGAAGTTATAGGCCAGAGATAAATAAATCTCGTTTATGAGCAACTTTGGCGCAATGAAACATTTAAGGAAAGAGCAATTGAAAATATGCTAGTTTGGATTTTATTGATTTATCTTGTGCTCATTATTCTTAGACATTGGCGTACCTTGGTAAATGAAGATAATGGTTTTATTAACAAGAATAACCAAAAAATTTGGTCCACAGTCAAAAGGAATACGGAGAGAGAGAGGCAAGGCAATAGAAAAGAAAAGAAATCTCAATGGGTCCAATCCATTCCATCAGCCACTATTGGTTGCTTGGGAGATGAGATGAGATGAGATGATGTAGTCGCTGCATGCCCATCAGCGCAGCGTTGAAATGTAAAATCAATGGCAGTGAAGATTGGAGAAGAGTGAAACAAATGCAAAGGGGAGAGTGGGTGAGTAAATGCGGTCCCTTTACAGCAGCCAGGCTAGTGATGGACAGTCTAAGGCTGTAGAATGGAAGAATAGGTCGTCCTTTGTATGTGATGTGGATACTCCAAATTTGTTTTCCCCCATTGTATTGTTCCTTTCACTGTTCTGCTGCAATCTGCCCTCTTACTCAGAACTTCTCTGCTCTCTCCTCTCTCGTTCCTGTCGGATAACTTATGACTCACCCACCCTTTCCTACACCAATTTATGCTATCTCTCTCTCGTCTCTCTCTTTCTGTTTCCCCTTGCACTTGCCTCTATTAAATGCTCATCTCTTCTCTCTCTCTCTCTCTCTTCCTCTCCCCTAGCCACTTTGTGGTTGTGGTGCAGTGGGTGGAAGTGGAACAAAGTAACAGAAAAATCTGGCAAATGGGTCTTTATTGCGTATGATTTTTACTCCTTTGCTTCGTTGGATCCATTAGTTCCGGGCATCAATGACTTGATGACTACCATGAATTTGCCTACTGTTTGGGCCACCTCATTACTTATTCTGCTTCTGCATCTTCTTCTTCTTCTCTCCGCCTCTTCTCCTCCCAGAGTATGCCTTCTCTCTCTCTTCCATGGCTTTCTCAATCTTTCCATGTTTGCTATTTCTTTTTAGTGACTGTTTCTGAGTTTTTTCTTCTCCTGGTTTCATCAGGGTATATTTTTTGAAGACAAAACCAGGCTTGGATCCACCCCACCAAGCTGCCATAACAAATGCAACGAGTGCCACCCATGCATGGCCGTTCAAGTGCCTAGCAATGCCCGGCCGCGCCAGCCGGTTGGATTCTCACTCGGCCTTGCCTATGAGATTTTTTGA

mRNA sequence

ATGGAAGCTACATGTAACACTTGCCCTATCAAAACCATACAATTTGGTGGCTACGGAAGTGGGTGGAAGTGGAACAAAGTAACAGAAAAATCTGGCAAATGGGTCTTTATTGCGTATGATTTTTACTCCTTTGCTTCGTTGGATCCATTAGTTCCGGGCATCAATGACTTGATGACTACCATGAATTTGCCTACTGTTTGGGCCACCTCATTACTTATTCTGCTTCTGCATCTTCTTCTTCTTCTCTCCGCCTCTTCTCCTCCCAGAGGTATATTTTTTGAAGACAAAACCAGGCTTGGATCCACCCCACCAAGCTGCCATAACAAATGCAACGAGTGCCACCCATGCATGGCCGTTCAAGTGCCTAGCAATGCCCGGCCGCGCCAGCCGGTTGGATTCTCACTCGGCCTTGCCTATGAGATTTTTTGA

Coding sequence (CDS)

ATGGAAGCTACATGTAACACTTGCCCTATCAAAACCATACAATTTGGTGGCTACGGAAGTGGGTGGAAGTGGAACAAAGTAACAGAAAAATCTGGCAAATGGGTCTTTATTGCGTATGATTTTTACTCCTTTGCTTCGTTGGATCCATTAGTTCCGGGCATCAATGACTTGATGACTACCATGAATTTGCCTACTGTTTGGGCCACCTCATTACTTATTCTGCTTCTGCATCTTCTTCTTCTTCTCTCCGCCTCTTCTCCTCCCAGAGGTATATTTTTTGAAGACAAAACCAGGCTTGGATCCACCCCACCAAGCTGCCATAACAAATGCAACGAGTGCCACCCATGCATGGCCGTTCAAGTGCCTAGCAATGCCCGGCCGCGCCAGCCGGTTGGATTCTCACTCGGCCTTGCCTATGAGATTTTTTGA

Protein sequence

MEATCNTCPIKTIQFGGYGSGWKWNKVTEKSGKWVFIAYDFYSFASLDPLVPGINDLMTTMNLPTVWATSLLILLLHLLLLLSASSPPRGIFFEDKTRLGSTPPSCHNKCNECHPCMAVQVPSNARPRQPVGFSLGLAYEIF

Homology

[Close]

BLAST of Bhi03G000893 vs. TAIR 10
Match: AT5G10310.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13898.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 67.8 bits (164), Expect = 8.1e-12
Identity = 33/69 (47.83%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 66  VWATSLLILLLHLLLLLSAS-----------SPPRGIFFEDKTRLGSTPPSCHNKCNECH 124
           ++ ++LL+L L L+LL++               P+    EDK RLGSTPPSCHN+CN CH
Sbjct: 4   IYKSTLLLLPLILILLITPQVSSFLQPIQPPISPQVALIEDKARLGSTPPSCHNRCNNCH 63

[Close]

BLAST of Bhi03G000893 vs. TAIR 10
Match: AT3G13898.1 (unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G10310.1). )

HSP 1 Score: 41.2 bits (95), Expect = 8.1e-04
Identity = 15/28 (53.57%), Postives = 19/28 (67.86%), Query Frame = 0


Query: 96  KTRLGSTPPSCHNKCNECHPCMAVQVPS 124
           + R+GS PPSC  KC  C PC A+Q P+
Sbjct: 54  RRRIGSKPPSCEKKCYGCEPCEAIQFPT 81

[Close]

BLAST of Bhi03G000893 vs. ExPASy Swiss-Prot
Match: Q9LFT5 (EPIDERMAL PATTERNING FACTOR-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=EPFL1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 33/69 (47.83%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 66  VWATSLLILLLHLLLLLSAS-----------SPPRGIFFEDKTRLGSTPPSCHNKCNECH 124
           ++ ++LL+L L L+LL++               P+    EDK RLGSTPPSCHN+CN CH
Sbjct: 4   IYKSTLLLLPLILILLITPQVSSFLQPIQPPISPQVALIEDKARLGSTPPSCHNRCNNCH 63

[Close]

BLAST of Bhi03G000893 vs. NCBI nr
Match: XP_008440584.1 (PREDICTED: EPIDERMAL PATTERNING FACTOR-like protein 1 [Cucumis melo])

HSP 1 Score: 157.1 bits (396), Expect = 1.1e-34
Identity = 76/85 (89.41%), Postives = 79/85 (92.94%), Query Frame = 0


Query: 39  YDFYSFASLDPLVPGINDLMTTMNLPTVWATSLLILLLHLLLLLSASSPPRGIFFEDKTR 98
           +   SFA LDP VPG+NDLMTTMNL TVWATSLLI+LLHLLLLLSASSPPRGIFFEDKTR
Sbjct: 3   FTLLSFALLDPWVPGMNDLMTTMNLATVWATSLLIVLLHLLLLLSASSPPRGIFFEDKTR 62

Query: 99  LGSTPPSCHNKCNECHPCMAVQVPS 124
           LGSTPPSCHNKCNECHPCMAVQVPS
Sbjct: 63  LGSTPPSCHNKCNECHPCMAVQVPS 87

[Close]

BLAST of Bhi03G000893 vs. NCBI nr
Match: KGN48738.1 (hypothetical protein Csa_003101 [Cucumis sativus])

HSP 1 Score: 138.3 bits (347), Expect = 5.2e-29
Identity = 66/70 (94.29%), Postives = 69/70 (98.57%), Query Frame = 0


Query: 54  INDLMTTMNLPTVWATSLLILLLHLLLLLSASSPPRGIFFEDKTRLGSTPPSCHNKCNEC 113
           +NDLMTTMNL TVWATSLLI+LLHLLLLLSASSPPRG+FFEDKTRLGSTPPSCHNKCNEC
Sbjct: 1   MNDLMTTMNLATVWATSLLIVLLHLLLLLSASSPPRGMFFEDKTRLGSTPPSCHNKCNEC 60

Query: 114 HPCMAVQVPS 124
           HPCMAVQVPS
Sbjct: 61  HPCMAVQVPS 70

[Close]

BLAST of Bhi03G000893 vs. NCBI nr
Match: KAG6604039.1 (EPIDERMAL PATTERNING FACTOR-like protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034205.1 EPIDERMAL PATTERNING FACTOR-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 121.3 bits (303), Expect = 6.6e-24
Identity = 59/69 (85.51%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 58  MTTMNLPTVWATSLLILLLHLLLLLSASSPPRGIFFEDKTRLGSTPPSCHNKCNECHPCM 117
           MTTMNL TVWATSLL++L+HLL LLSAS PP GI FEDKTRLGSTPPSCHNKCNECHPCM
Sbjct: 1   MTTMNLATVWATSLLLVLVHLLPLLSASPPPIGISFEDKTRLGSTPPSCHNKCNECHPCM 60

Query: 118 AVQVPSNAR 127
           AVQVPS  R
Sbjct: 61  AVQVPSMPR 69

[Close]

BLAST of Bhi03G000893 vs. NCBI nr
Match: XP_010025427.2 (EPIDERMAL PATTERNING FACTOR-like protein 1 [Eucalyptus grandis])

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-12
Identity = 40/52 (76.92%), Postives = 44/52 (84.62%), Query Frame = 0


Query: 72  LILLLHLLLLLSASSPPRGIFFEDKTRLGSTPPSCHNKCNECHPCMAVQVPS 124
           L+LLL LLLL   S P +G+ FE+KTRLGSTPPSCHNKCN CHPCMAVQVPS
Sbjct: 24  LLLLLCLLLLPPPSFPRQGLSFEEKTRLGSTPPSCHNKCNGCHPCMAVQVPS 75

[Close]

BLAST of Bhi03G000893 vs. NCBI nr
Match: OVA07949.1 (hypothetical protein BVC80_8733g16 [Macleaya cordata])

HSP 1 Score: 82.8 bits (203), Expect = 2.6e-12
Identity = 38/83 (45.78%), Postives = 58/83 (69.88%), Query Frame = 0


Query: 58  MTTMNLPTVWATSLLILLLHLLLLLSA-------SSPPRGIFFEDKTRLGSTPPSCHNKC 117
           M  +N   +++T++L+ +LHLL   ++       S+PP+G+  E K+RLGSTPPSC+N+C
Sbjct: 1   MRLLNPDLLYSTTILVTVLHLLFFPASCIDQQPISAPPQGVLMEGKSRLGSTPPSCNNRC 60

Query: 118 NECHPCMAVQVPSNARPRQPVGF 134
           N+C+PCMA+QVP+  R R   GF
Sbjct: 61  NQCNPCMAIQVPTQTRDRVKPGF 83

[Close]

BLAST of Bhi03G000893 vs. ExPASy TrEMBL
Match: A0A1S3B112 (Epidermal patterning factor-like protein OS=Cucumis melo OX=3656 GN=LOC103484960 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 5.3e-35
Identity = 76/85 (89.41%), Postives = 79/85 (92.94%), Query Frame = 0


Query: 39  YDFYSFASLDPLVPGINDLMTTMNLPTVWATSLLILLLHLLLLLSASSPPRGIFFEDKTR 98
           +   SFA LDP VPG+NDLMTTMNL TVWATSLLI+LLHLLLLLSASSPPRGIFFEDKTR
Sbjct: 3   FTLLSFALLDPWVPGMNDLMTTMNLATVWATSLLIVLLHLLLLLSASSPPRGIFFEDKTR 62

Query: 99  LGSTPPSCHNKCNECHPCMAVQVPS 124
           LGSTPPSCHNKCNECHPCMAVQVPS
Sbjct: 63  LGSTPPSCHNKCNECHPCMAVQVPS 87

[Close]

BLAST of Bhi03G000893 vs. ExPASy TrEMBL
Match: A0A0A0KGR1 (Epidermal patterning factor-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G499820 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.5e-29
Identity = 66/70 (94.29%), Postives = 69/70 (98.57%), Query Frame = 0


Query: 54  INDLMTTMNLPTVWATSLLILLLHLLLLLSASSPPRGIFFEDKTRLGSTPPSCHNKCNEC 113
           +NDLMTTMNL TVWATSLLI+LLHLLLLLSASSPPRG+FFEDKTRLGSTPPSCHNKCNEC
Sbjct: 1   MNDLMTTMNLATVWATSLLIVLLHLLLLLSASSPPRGMFFEDKTRLGSTPPSCHNKCNEC 60

Query: 114 HPCMAVQVPS 124
           HPCMAVQVPS
Sbjct: 61  HPCMAVQVPS 70

[Close]

BLAST of Bhi03G000893 vs. ExPASy TrEMBL
Match: A0A200QBV8 (Epidermal patterning factor-like protein OS=Macleaya cordata OX=56857 GN=BVC80_8733g16 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-12
Identity = 38/83 (45.78%), Postives = 58/83 (69.88%), Query Frame = 0


Query: 58  MTTMNLPTVWATSLLILLLHLLLLLSA-------SSPPRGIFFEDKTRLGSTPPSCHNKC 117
           M  +N   +++T++L+ +LHLL   ++       S+PP+G+  E K+RLGSTPPSC+N+C
Sbjct: 1   MRLLNPDLLYSTTILVTVLHLLFFPASCIDQQPISAPPQGVLMEGKSRLGSTPPSCNNRC 60

Query: 118 NECHPCMAVQVPSNARPRQPVGF 134
           N+C+PCMA+QVP+  R R   GF
Sbjct: 61  NQCNPCMAIQVPTQTRDRVKPGF 83

[Close]

BLAST of Bhi03G000893 vs. ExPASy TrEMBL
Match: A0A2C9UW43 (Epidermal patterning factor-like protein OS=Manihot esculenta OX=3983 GN=MANES_12G132000 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.7e-12
Identity = 42/59 (71.19%), Postives = 46/59 (77.97%), Query Frame = 0


Query: 68  ATSLLILLLHLLLLLSASSP---PRGIFFEDKTRLGSTPPSCHNKCNECHPCMAVQVPS 124
           A SL +LLL LL L S S P   PRG+  E+KTRLGSTPPSCHNKCN CHPCMAVQVP+
Sbjct: 27  AASLCLLLLLLLPLASCSHPRPQPRGLLSEEKTRLGSTPPSCHNKCNGCHPCMAVQVPT 85

[Close]

BLAST of Bhi03G000893 vs. ExPASy TrEMBL
Match: A0A165XQV6 (Epidermal patterning factor-like protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_014212 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.8e-12
Identity = 40/64 (62.50%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 72  LILLLHLLL----LLSASSPPRGIFFEDKTRLGSTPPSCHNKCNECHPCMAVQVPSNARP 131
           +ILLLHLLL     L +     G+ FE+KTRLGSTPPSCHNKCN+CHPCMA QVPS    
Sbjct: 8   IILLLHLLLSPSFCLDSQDSTEGLTFEEKTRLGSTPPSCHNKCNQCHPCMAAQVPSLTTQ 67

The following BLAST results are available for this feature:

BLAST of Bhi03G000893 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10310.1	8.1e-12	47.83	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G13898.1	8.1e-04	53.57	unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana prot...	[more]

BLAST of Bhi03G000893 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFT5	1.1e-10	47.83	EPIDERMAL PATTERNING FACTOR-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=EP...	[more]

BLAST of Bhi03G000893 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008440584.1	1.1e-34	89.41	PREDICTED: EPIDERMAL PATTERNING FACTOR-like protein 1 [Cucumis melo]	[more]
KGN48738.1	5.2e-29	94.29	hypothetical protein Csa_003101 [Cucumis sativus]	[more]
KAG6604039.1	6.6e-24	85.51	EPIDERMAL PATTERNING FACTOR-like protein 1, partial [Cucurbita argyrosperma subs...	[more]
XP_010025427.2	2.0e-12	76.92	EPIDERMAL PATTERNING FACTOR-like protein 1 [Eucalyptus grandis]	[more]
OVA07949.1	2.6e-12	45.78	hypothetical protein BVC80_8733g16 [Macleaya cordata]	[more]

BLAST of Bhi03G000893 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B112	5.3e-35	89.41	Epidermal patterning factor-like protein OS=Cucumis melo OX=3656 GN=LOC103484960...	[more]
A0A0A0KGR1	2.5e-29	94.29	Epidermal patterning factor-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G499...	[more]
A0A200QBV8	1.3e-12	45.78	Epidermal patterning factor-like protein OS=Macleaya cordata OX=56857 GN=BVC80_8...	[more]
A0A2C9UW43	1.7e-12	71.19	Epidermal patterning factor-like protein OS=Manihot esculenta OX=3983 GN=MANES_1...	[more]
A0A165XQV6	2.8e-12	62.50	Epidermal patterning factor-like protein OS=Daucus carota subsp. sativus OX=7920...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF17181	EPF	coord: 98..122 e-value: 7.7E-11 score: 41.8
None	No IPR available	PANTHER	PTHR33109:SF3	EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 1	coord: 67..126
IPR039455	EPIDERMAL PATTERNING FACTOR-like protein	PANTHER	PTHR33109	EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4	coord: 67..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000893	Bhi03M000893	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010052	guard cell differentiation
biological_process	GO:0010374	stomatal complex development
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane