Bhi03G000843 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000843
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionBasic helix-loop-helix transcription factor
Locationchr3: 22418770 .. 22421519 (-)
RNA-Seq ExpressionBhi03G000843
SyntenyBhi03G000843
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAATATTAATCAAATTGCTCTTTCTAATTAAGTTTGATCCCATTGAGTTCAGTACTGCTATGGAGATCTCATCAGCCAGATGGATATCTGAATGGGTAATTTTAATTACCTCTAATTTTTATTCAATTAATTAAAATATATATTTATCTTTTGGTGAATGGTTTTGGATTTTTTTTTTTAGGAAATTGAAGATCCTCCGTCGTTTATGGATGAATTTAAGGTAGACCCTTTTGATTTTTCGTTGGATGAGTTCAAATTTGAGGAGTACTCTGGTGGGAGTTGGAGTGGGACTGACTTTTATGAGTCGCCGGTTCCGCCGTCTGCGGTGACGGAAAGGCCGACAAAGCAATTGAAGGTGGCGGTGGACGGGGGGATTAAATGTCAAATATTTGATGGCAATTATAAGGCAGATTCGTCATCATCCACTTCCCAAATAATTAGCTTCGAAAATTTTTCGAATTCAAAATGTGGGAAAGGCAGTGTGGGGGCCAAAATTGAGATGGATTATGAATCGGTAAATATTAGTGAAAGTTATTGGAATAATAATGAGAAAAGTTGGAAGAAATGTTCGTCGTCGATGAATAGAAATCCTTTGCATGCTCGAGAACATGTCATAGCTGAGAGAAAACGTAGAGAGAAACTTAGCCAACGATTCATTGCTCTTTCAGCTCTCATCCCTGGCCTTAACAAGGTGTCGTACTATACTTTGCCTCATTACAAATTTTCCATTCTAATTTTTTTATATACTTGTGTGTCTGTATTTAATTTTTCAATTACTCTAAGAATGTAATGGTCAAATTAGAAAGAAAGCTATAAAGGTTTGTATTTGTTCGTTCTTACTTTCTCAAATGGGTCAAAGTTTGAGAGACGGTAAAAACTGTCTAATACTTAGGTTTTAGTTTTATCTCACACTCTAACTTTCAGTTGTTTGTGTAATGGATTTTTATTACATCTCACATATTTTTAAGTTTTCAAATATATTAGACACAAAAATGTTAGTTTAAGAATTTATCAGACATGTTTTTTTTTAATATACAGTAAAAATTGATTAGACAGAGAATTCAAATAGTATGAAAACCATTTTGAATTTCTAGTTTTGGAAAATTGGGTTTTCATTTTCGTTTAATTTCTTTGGAGTGGCTTTTATGTAACTTTCTTATAGGAAGATTTGAATTTATAGTCATATTCTTAAAAACAGAAGATTTAAGTTGGTTTAGTAATCATTTTGTTTTCAAAAATTAAAACATTAACTTTATCTTTAAGGTCTCATTTTGTAATTATTTTATTTTTTGTTTTTGTTTTTTAAAATTAAGTCTATGAACATTACTTCCACCTTCAAATTTCTCCCTTGGTAATTTACTTTTCATCATTGGTTTACAAAACCAAACCAAATTTTGAGAACTAAAAAAACGTAATTTTCAAAATATTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNTGTAAATCCTTTAAGAAATATTGATGAAATAAACTTAATTTCAAAAAAAAAAAAAAAAAAATATCACTAAATGGAACCTAAGTTTCTTGGTTTTGTTATTTGCATTTCACTAACTAGTTAGAAATCTAGCCAAAATTGAAAACTAATGAAAAAAAGTTGATAAGATTTAAATGTTTTTTTTTTAAAGAAAGGGTTAGAACTAAAGTTTGGAATTTAGGAGAAAAATAAGCATAAATTTCTTTTAAAAAAAGAAATAAAGATAAAATTAAGTTATTCAAGTGGATCTACTCATTCACGTAATATCTTCTTCTAAAAAAAGGATAAAATCGTAGCCTTATTCTATTTCATTTGTGTTGGCAGGTAGACAAAGCTTCGATCTTGGGAGGAGCCATAAGACATGTGAAAGAACTTCAAGAGCGTTTGAAAGTGGTGGAGGAACAACCAAATTCCAAAATGTCGGAGGCACAATCGACTGCGTGTGTAAAAAGAACAAGCCTCCAGGTTTCCTCCCCCGACGATGAGTCGGACGATAACAGTTTTACTGGCGGGCCAGCTCCGGAGATAGAAGCAAGGTTCTCAAACAAGGATGTTCTTATCAGAATCCACTGCCAAAAACGCAAAGGTTGTCTTTCCTATTTGCTTAACAAAATTGAGAACCTCAATAATCTAACAATCCTCAACACTTCTGCCTTGCCATTTGGCCACTCCAAGCTCGATATCACTATTGTTGCTCAGGTAATTTATATATGATTTTAAGCTTTTTATTTTAATTAAATTTTTTTATTGATACATATCATTTATCTGATTAATTTGCTGAGGAGATTAATGTTTTTGTTTGTTTAATGATGGCAGGTGGATGTTGGTTTTTGTTTGACAGTGGAGGATGTTGTGAAGAATCTGAGACAAGCTTTGCTGGACTTCCATTAAAACTTTGGAAAAAGACGAGTCTTTTTTAGATTGATTAATCTTTCTTTCCCTTTTTCCGATAAAAAAAATTATTTAAAAAAAAAAAAAAAACTGTAAATTTTAATGTAATAAAAACCCATTTCAGAGGGGTAAATTTTTTTTGAGAGCTATGGACTTTTATTTGTCTCACCTCTTTCTTCTATTTTTTTCCCTCTGTTAAAAATGGTTTTTTTTTTTTTTTTTTTCTGGAGTACTTTTTGCAGGCTATTTTCTCTCTTGGTAGGGTTTTAGGTTTGGTTTTTGGAGGGCCCTTGTGTCTGTAATGTAGAAGAAACTCGGATTCTTATGTTTTAATTAGATTTTCTCCTTTTAATA

mRNA sequence

ACAATATTAATCAAATTGCTCTTTCTAATTAAGTTTGATCCCATTGAGTTCAGTACTGCTATGGAGATCTCATCAGCCAGATGGATATCTGAATGGGAAATTGAAGATCCTCCGTCGTTTATGGATGAATTTAAGGTAGACCCTTTTGATTTTTCGTTGGATGAGTTCAAATTTGAGGAGTACTCTGGTGGGAGTTGGAGTGGGACTGACTTTTATGAGTCGCCGGTTCCGCCGTCTGCGGTGACGGAAAGGCCGACAAAGCAATTGAAGGTGGCGGTGGACGGGGGGATTAAATGTCAAATATTTGATGGCAATTATAAGGCAGATTCGTCATCATCCACTTCCCAAATAATTAGCTTCGAAAATTTTTCGAATTCAAAATGTGGGAAAGGCAGTGTGGGGGCCAAAATTGAGATGGATTATGAATCGGTAAATATTAGTGAAAGTTATTGGAATAATAATGAGAAAAGTTGGAAGAAATGTTCGTCGTCGATGAATAGAAATCCTTTGCATGCTCGAGAACATGTCATAGCTGAGAGAAAACGTAGAGAGAAACTTAGCCAACGATTCATTGCTCTTTCAGCTCTCATCCCTGGCCTTAACAAGGTAGACAAAGCTTCGATCTTGGGAGGAGCCATAAGACATGTGAAAGAACTTCAAGAGCGTTTGAAAGTGGTGGAGGAACAACCAAATTCCAAAATGTCGGAGGCACAATCGACTGCGTGTGTAAAAAGAACAAGCCTCCAGGTTTCCTCCCCCGACGATGAGTCGGACGATAACAGTTTTACTGGCGGGCCAGCTCCGGAGATAGAAGCAAGGTTCTCAAACAAGGATGTTCTTATCAGAATCCACTGCCAAAAACGCAAAGGTTGTCTTTCCTATTTGCTTAACAAAATTGAGAACCTCAATAATCTAACAATCCTCAACACTTCTGCCTTGCCATTTGGCCACTCCAAGCTCGATATCACTATTGTTGCTCAGGTGGATGTTGGTTTTTGTTTGACAGTGGAGGATGTTGTGAAGAATCTGAGACAAGCTTTGCTGGACTTCCATTAAAACTTTGGAAAAAGACGAGTCTTTTTTAGATTGATTAATCTTTCTTTCCCTTTTTCCGATAAAAAAAATTATTTAAAAAAAAAAAAAAAACTGTAAATTTTAATGTAATAAAAACCCATTTCAGAGGGGTAAATTTTTTTTGAGAGCTATGGACTTTTATTTGTCTCACCTCTTTCTTCTATTTTTTTCCCTCTGTTAAAAATGGTTTTTTTTTTTTTTTTTTTCTGGAGTACTTTTTGCAGGCTATTTTCTCTCTTGGTAGGGTTTTAGGTTTGGTTTTTGGAGGGCCCTTGTGTCTGTAATGTAGAAGAAACTCGGATTCTTATGTTTTAATTAGATTTTCTCCTTTTAATA

Coding sequence (CDS)

ATGGAGATCTCATCAGCCAGATGGATATCTGAATGGGAAATTGAAGATCCTCCGTCGTTTATGGATGAATTTAAGGTAGACCCTTTTGATTTTTCGTTGGATGAGTTCAAATTTGAGGAGTACTCTGGTGGGAGTTGGAGTGGGACTGACTTTTATGAGTCGCCGGTTCCGCCGTCTGCGGTGACGGAAAGGCCGACAAAGCAATTGAAGGTGGCGGTGGACGGGGGGATTAAATGTCAAATATTTGATGGCAATTATAAGGCAGATTCGTCATCATCCACTTCCCAAATAATTAGCTTCGAAAATTTTTCGAATTCAAAATGTGGGAAAGGCAGTGTGGGGGCCAAAATTGAGATGGATTATGAATCGGTAAATATTAGTGAAAGTTATTGGAATAATAATGAGAAAAGTTGGAAGAAATGTTCGTCGTCGATGAATAGAAATCCTTTGCATGCTCGAGAACATGTCATAGCTGAGAGAAAACGTAGAGAGAAACTTAGCCAACGATTCATTGCTCTTTCAGCTCTCATCCCTGGCCTTAACAAGGTAGACAAAGCTTCGATCTTGGGAGGAGCCATAAGACATGTGAAAGAACTTCAAGAGCGTTTGAAAGTGGTGGAGGAACAACCAAATTCCAAAATGTCGGAGGCACAATCGACTGCGTGTGTAAAAAGAACAAGCCTCCAGGTTTCCTCCCCCGACGATGAGTCGGACGATAACAGTTTTACTGGCGGGCCAGCTCCGGAGATAGAAGCAAGGTTCTCAAACAAGGATGTTCTTATCAGAATCCACTGCCAAAAACGCAAAGGTTGTCTTTCCTATTTGCTTAACAAAATTGAGAACCTCAATAATCTAACAATCCTCAACACTTCTGCCTTGCCATTTGGCCACTCCAAGCTCGATATCACTATTGTTGCTCAGGTGGATGTTGGTTTTTGTTTGACAGTGGAGGATGTTGTGAAGAATCTGAGACAAGCTTTGCTGGACTTCCATTAA

Protein sequence

MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSAVTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIEMDYESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSSPDDESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH
Homology
BLAST of Bhi03G000843 vs. TAIR 10
Match: AT2G22770.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 4.3e-40
Identity = 125/341 (36.66%), Postives = 182/341 (53.37%), Query Frame = 0

Query: 16  DPPSFMDEFKVDPFDFSLDEFKFE---------EYSGGSWSGTDFYESPVPPSAVTERPT 75
           D  SFMD   +D  ++ +D+++ +              S SGT F         + ERPT
Sbjct: 2   DDSSFMD-LMIDTDEYLIDDWESDFPICGETNTNPGSESGSGTGF-------ELLAERPT 61

Query: 76  KQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIEMDYESVNI 135
           KQ+K   +  +       +  + S S TSQ+ISF          GS   K      S+N 
Sbjct: 62  KQMK--TNNNMNSTSSSPSSSSSSGSRTSQVISF----------GSPDTKTNPVETSLNF 121

Query: 136 SESYWNNNEKSWKKCSSSMN----RNPLHAREHVIAERKRREKLSQRFIALSALIPGLNK 195
           S    + ++K   K    +N    R P   +EHV+AERKRR+KL++R IALSAL+PGL K
Sbjct: 122 SNQV-SMDQKVGSKRKDCVNNGGRREPHLLKEHVLAERKRRQKLNERLIALSALLPGLKK 181

Query: 196 VDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSL-------------Q 255
            DKA++L  AI+H+K+LQER+K +EE+        QS   VKR+ +              
Sbjct: 182 TDKATVLEDAIKHLKQLQERVKKLEEERVVTKKMDQSIILVKRSQVYLDDDSSSYSSTCS 241

Query: 256 VSSP-DDESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTIL 315
            +SP    SD+ S      P IEAR S++D+LIR+HC+K KGC+  +L+ +E    L ++
Sbjct: 242 AASPLSSSSDEVSIFKQTMPMIEARVSDRDLLIRVHCEKNKGCMIKILSSLEKF-RLEVV 301

Query: 316 NTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLD 330
           N+  LPFG+S L ITI+ ++D  F   VE+VVKN+R AL +
Sbjct: 302 NSFTLPFGNSTLVITILTKMDNKFSRPVEEVVKNIRVALAE 320

BLAST of Bhi03G000843 vs. TAIR 10
Match: AT4G37850.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-38
Identity = 121/341 (35.48%), Postives = 192/341 (56.30%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           M I S RW SE EIE+  S + +F ++     + E ++      + +    Y+       
Sbjct: 1   MSILSTRWFSEQEIEE-NSIIQQFHMNSIVGEVQEAQYIFPHSFTTNNDPSYD-----DL 60

Query: 61  VTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG--------KGS 120
           +  +P K L+            +         S+S+I+SFE++ ++              
Sbjct: 61  IEMKPPKILETTYISPSSHLPPNSKPHHIHRHSSSRILSFEDYGSNDMEHEYSPTYLNSI 120

Query: 121 VGAKIEMDYESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIA 180
              K+E   +    S+ +   N K  K+ +   +RN  +A++H+IAERKRREKL+QRF+A
Sbjct: 121 FSPKLEAQVQPHQKSDEF---NRKGTKR-AQPFSRNQSNAQDHIIAERKRREKLTQRFVA 180

Query: 181 LSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSS 240
           LSAL+PGL K+DKAS+LG A++H+K LQER+  +EEQ   K    +S   VK++ L +  
Sbjct: 181 LSALVPGLKKMDKASVLGDALKHIKYLQERVGELEEQ--KKERRLESMVLVKKSKLILDD 240

Query: 241 PD---DESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILN 300
            +     S ++ F+    PEIE RFS++DVLI+I C+K+KG L+ ++ +IE L ++ I N
Sbjct: 241 NNQSFSSSCEDGFSDLDLPEIEVRFSDEDVLIKILCEKQKGHLAKIMAEIEKL-HILITN 300

Query: 301 TSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDF 331
           +S L FG + LDITI+A+ +  F +T+ DVVK+LR AL +F
Sbjct: 301 SSVLNFGPT-LDITIIAKKESDFDMTLMDVVKSLRSALSNF 327

BLAST of Bhi03G000843 vs. TAIR 10
Match: AT2G22750.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-38
Identity = 110/271 (40.59%), Postives = 152/271 (56.09%), Query Frame = 0

Query: 59  SAVTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIE 118
           S V ERP+K LK              N        +S+I+SFE          S      
Sbjct: 31  SLVEERPSKILKTTHISPNLHPFSSSNPPPPKHQPSSRILSFEKTGLHVMNHNSPNLIFS 90

Query: 119 MDYESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIP 178
              E + + E           K + S+ R+  +A++H++AERKRREKL+QRF+ALSALIP
Sbjct: 91  PKDEEIGLPEHKKAELIIRGTKRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIP 150

Query: 179 GLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSL------QVSS 238
           GL K+DKAS+LG AI+H+K LQE +K  EEQ   K  E  S   VK++SL      Q SS
Sbjct: 151 GLKKMDKASVLGDAIKHIKYLQESVKEYEEQKKEKTME--SVVLVKKSSLVLDENHQPSS 210

Query: 239 PDDESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSA 298
                 + + +    PEIE R S KDVLI+I C+K+KG +  ++ +IE L  L+I N++ 
Sbjct: 211 SSSSDGNRNSSSSNLPEIEVRVSGKDVLIKILCEKQKGNVIKIMGEIEKL-GLSITNSNV 270

Query: 299 LPFGHSKLDITIVAQVDVGFCLTVEDVVKNL 324
           LPFG +  DI+I+AQ +  F + +EDVVKNL
Sbjct: 271 LPFGPT-FDISIIAQKNNNFDMKIEDVVKNL 297

BLAST of Bhi03G000843 vs. TAIR 10
Match: AT2G22750.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 153.7 bits (387), Expect = 2.6e-37
Identity = 110/271 (40.59%), Postives = 152/271 (56.09%), Query Frame = 0

Query: 59  SAVTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIE 118
           S V ERP+K LK              N        +S+I+SFE          S      
Sbjct: 31  SLVEERPSKILKTTHISPNLHPFSSSNPPPPKHQPSSRILSFEKTGLHVMNHNSPNLIFS 90

Query: 119 MDYESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIP 178
              E + + E           K + S+ R+  +A++H++AERKRREKL+QRF+ALSALIP
Sbjct: 91  PKDEEIGLPEHKKAELIIRGTKRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIP 150

Query: 179 GLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSL------QVSS 238
           GL K+DKAS+LG AI+H+K LQE +K  EEQ   K  E  S   VK++SL      Q SS
Sbjct: 151 GLKKMDKASVLGDAIKHIKYLQESVKEYEEQKKEKTME--SVVLVKKSSLVLDENHQPSS 210

Query: 239 PDDESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSA 298
                 + + +    PEIE R S KDVLI+I C+K+KG +  ++ +IE L  L+I N++ 
Sbjct: 211 SSSSDGNRNSSSSNLPEIEVRVSGKDVLIKILCEKQKGNVIKIMGEIEKL-GLSITNSNV 270

Query: 299 LPFGHSKLDITIVAQVDVGFCLTVEDVVKNL 324
           LPFG +  DI+I+AQ +  F + +EDVVKNL
Sbjct: 271 LPFGPT-FDISIIAQ-NNNFDMKIEDVVKNL 296

BLAST of Bhi03G000843 vs. TAIR 10
Match: AT2G22760.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.9e-35
Identity = 89/194 (45.88%), Postives = 129/194 (66.49%), Query Frame = 0

Query: 139 KKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIPGLNKVDKASILGGAIRHVKE 198
           +K  S   R+P+ A+EHV+AERKRREKLS++FIALSAL+PGL K DK +IL  AI  +K+
Sbjct: 104 RKTCSHGTRSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQ 163

Query: 199 LQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSSPDDESDDNSF---TGGPAPEIEARFS 258
           LQE+L+ ++E+  +   + +S   VK++ +      + S   S         PEIEA+ S
Sbjct: 164 LQEQLRTLKEEKEA-TRQMESMILVKKSKVFFDEEPNLSCSPSVHIEFDQALPEIEAKIS 223

Query: 259 NKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSALPFGHSKLDITIVAQVDVGFCLT 318
             D+LIRI C+K KGC+  +LN IEN   L I N+  LPFG S LDIT++AQ+D  F ++
Sbjct: 224 QNDILIRILCEKSKGCMINILNTIENF-QLRIENSIVLPFGDSTLDITVLAQMDKDFSMS 283

Query: 319 V-EDVVKNLRQALL 329
           + +D+V+NLR A++
Sbjct: 284 ILKDLVRNLRLAMV 295

BLAST of Bhi03G000843 vs. ExPASy Swiss-Prot
Match: Q8S3F1 (Transcription factor NAI1 OS=Arabidopsis thaliana OX=3702 GN=NAI1 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.1e-39
Identity = 125/341 (36.66%), Postives = 182/341 (53.37%), Query Frame = 0

Query: 16  DPPSFMDEFKVDPFDFSLDEFKFE---------EYSGGSWSGTDFYESPVPPSAVTERPT 75
           D  SFMD   +D  ++ +D+++ +              S SGT F         + ERPT
Sbjct: 2   DDSSFMD-LMIDTDEYLIDDWESDFPICGETNTNPGSESGSGTGF-------ELLAERPT 61

Query: 76  KQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIEMDYESVNI 135
           KQ+K   +  +       +  + S S TSQ+ISF          GS   K      S+N 
Sbjct: 62  KQMK--TNNNMNSTSSSPSSSSSSGSRTSQVISF----------GSPDTKTNPVETSLNF 121

Query: 136 SESYWNNNEKSWKKCSSSMN----RNPLHAREHVIAERKRREKLSQRFIALSALIPGLNK 195
           S    + ++K   K    +N    R P   +EHV+AERKRR+KL++R IALSAL+PGL K
Sbjct: 122 SNQV-SMDQKVGSKRKDCVNNGGRREPHLLKEHVLAERKRRQKLNERLIALSALLPGLKK 181

Query: 196 VDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSL-------------Q 255
            DKA++L  AI+H+K+LQER+K +EE+        QS   VKR+ +              
Sbjct: 182 TDKATVLEDAIKHLKQLQERVKKLEEERVVTKKMDQSIILVKRSQVYLDDDSSSYSSTCS 241

Query: 256 VSSP-DDESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTIL 315
            +SP    SD+ S      P IEAR S++D+LIR+HC+K KGC+  +L+ +E    L ++
Sbjct: 242 AASPLSSSSDEVSIFKQTMPMIEARVSDRDLLIRVHCEKNKGCMIKILSSLEKF-RLEVV 301

Query: 316 NTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLD 330
           N+  LPFG+S L ITI+ ++D  F   VE+VVKN+R AL +
Sbjct: 302 NSFTLPFGNSTLVITILTKMDNKFSRPVEEVVKNIRVALAE 320

BLAST of Bhi03G000843 vs. ExPASy Swiss-Prot
Match: Q9T072 (Transcription factor bHLH25 OS=Arabidopsis thaliana OX=3702 GN=BHLH25 PE=2 SV=2)

HSP 1 Score: 157.9 bits (398), Expect = 2.0e-37
Identity = 121/341 (35.48%), Postives = 192/341 (56.30%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           M I S RW SE EIE+  S + +F ++     + E ++      + +    Y+       
Sbjct: 1   MSILSTRWFSEQEIEE-NSIIQQFHMNSIVGEVQEAQYIFPHSFTTNNDPSYD-----DL 60

Query: 61  VTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG--------KGS 120
           +  +P K L+            +         S+S+I+SFE++ ++              
Sbjct: 61  IEMKPPKILETTYISPSSHLPPNSKPHHIHRHSSSRILSFEDYGSNDMEHEYSPTYLNSI 120

Query: 121 VGAKIEMDYESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIA 180
              K+E   +    S+ +   N K  K+ +   +RN  +A++H+IAERKRREKL+QRF+A
Sbjct: 121 FSPKLEAQVQPHQKSDEF---NRKGTKR-AQPFSRNQSNAQDHIIAERKRREKLTQRFVA 180

Query: 181 LSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSS 240
           LSAL+PGL K+DKAS+LG A++H+K LQER+  +EEQ   K    +S   VK++ L +  
Sbjct: 181 LSALVPGLKKMDKASVLGDALKHIKYLQERVGELEEQ--KKERRLESMVLVKKSKLILDD 240

Query: 241 PD---DESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILN 300
            +     S ++ F+    PEIE RFS++DVLI+I C+K+KG L+ ++ +IE L ++ I N
Sbjct: 241 NNQSFSSSCEDGFSDLDLPEIEVRFSDEDVLIKILCEKQKGHLAKIMAEIEKL-HILITN 300

Query: 301 TSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDF 331
           +S L FG + LDITI+A+ +  F +T+ DVVK+LR AL +F
Sbjct: 301 SSVLNFGPT-LDITIIAKKESDFDMTLMDVVKSLRSALSNF 327

BLAST of Bhi03G000843 vs. ExPASy Swiss-Prot
Match: Q1PF17 (Transcription factor bHLH18 OS=Arabidopsis thaliana OX=3702 GN=BHLH18 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.3e-37
Identity = 110/271 (40.59%), Postives = 152/271 (56.09%), Query Frame = 0

Query: 59  SAVTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIE 118
           S V ERP+K LK              N        +S+I+SFE          S      
Sbjct: 31  SLVEERPSKILKTTHISPNLHPFSSSNPPPPKHQPSSRILSFEKTGLHVMNHNSPNLIFS 90

Query: 119 MDYESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIP 178
              E + + E           K + S+ R+  +A++H++AERKRREKL+QRF+ALSALIP
Sbjct: 91  PKDEEIGLPEHKKAELIIRGTKRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIP 150

Query: 179 GLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSL------QVSS 238
           GL K+DKAS+LG AI+H+K LQE +K  EEQ   K  E  S   VK++SL      Q SS
Sbjct: 151 GLKKMDKASVLGDAIKHIKYLQESVKEYEEQKKEKTME--SVVLVKKSSLVLDENHQPSS 210

Query: 239 PDDESDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSA 298
                 + + +    PEIE R S KDVLI+I C+K+KG +  ++ +IE L  L+I N++ 
Sbjct: 211 SSSSDGNRNSSSSNLPEIEVRVSGKDVLIKILCEKQKGNVIKIMGEIEKL-GLSITNSNV 270

Query: 299 LPFGHSKLDITIVAQVDVGFCLTVEDVVKNL 324
           LPFG +  DI+I+AQ +  F + +EDVVKNL
Sbjct: 271 LPFGPT-FDISIIAQKNNNFDMKIEDVVKNL 297

BLAST of Bhi03G000843 vs. ExPASy Swiss-Prot
Match: Q1PF16 (Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.6e-34
Identity = 89/194 (45.88%), Postives = 129/194 (66.49%), Query Frame = 0

Query: 139 KKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIPGLNKVDKASILGGAIRHVKE 198
           +K  S   R+P+ A+EHV+AERKRREKLS++FIALSAL+PGL K DK +IL  AI  +K+
Sbjct: 104 RKTCSHGTRSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQ 163

Query: 199 LQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSSPDDESDDNSF---TGGPAPEIEARFS 258
           LQE+L+ ++E+  +   + +S   VK++ +      + S   S         PEIEA+ S
Sbjct: 164 LQEQLRTLKEEKEA-TRQMESMILVKKSKVFFDEEPNLSCSPSVHIEFDQALPEIEAKIS 223

Query: 259 NKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSALPFGHSKLDITIVAQVDVGFCLT 318
             D+LIRI C+K KGC+  +LN IEN   L I N+  LPFG S LDIT++AQ+D  F ++
Sbjct: 224 QNDILIRILCEKSKGCMINILNTIENF-QLRIENSIVLPFGDSTLDITVLAQMDKDFSMS 283

Query: 319 V-EDVVKNLRQALL 329
           + +D+V+NLR A++
Sbjct: 284 ILKDLVRNLRLAMV 295

BLAST of Bhi03G000843 vs. ExPASy Swiss-Prot
Match: Q9ZPY8 (Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Arabidopsis thaliana OX=3702 GN=AIB PE=2 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 4.0e-14
Identity = 48/141 (34.04%), Postives = 82/141 (58.16%), Query Frame = 0

Query: 147 RNPLHARE----HVIAERKRREKLSQRFIALSALIPGLNKVDKASILGGAIRHVKELQER 206
           R P + RE    HV AER+RREKL+QRF AL +++P ++K+DKAS+LG AI ++KELQE+
Sbjct: 384 RKPANGREEPLNHVEAERQRREKLNQRFYALRSVVPNISKMDKASLLGDAISYIKELQEK 443

Query: 207 LKVVEEQPNSKMSEAQSTACVKRTSLQVSSPDDESDDNSFTGGPAPEIEARFSNKDVLIR 266
           +K++E++                   +V +    S+ N+ T   +PE++ +  N++V++R
Sbjct: 444 VKIMEDE-------------------RVGTDKSLSESNTITVEESPEVDIQAMNEEVVVR 503

Query: 267 IHCQKRKGCLSYLLNKIENLN 284
           +         S ++  + N N
Sbjct: 504 VISPLDSHPASRIIQAMRNSN 505

BLAST of Bhi03G000843 vs. NCBI nr
Match: XP_038883438.1 (transcription factor bHLH18-like [Benincasa hispida])

HSP 1 Score: 652.9 bits (1683), Expect = 1.5e-183
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA
Sbjct: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60

Query: 61  VTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIEMD 120
           VTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIEMD
Sbjct: 61  VTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIEMD 120

Query: 121 YESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIPGL 180
           YESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIPGL
Sbjct: 121 YESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQRFIALSALIPGL 180

Query: 181 NKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSSPDDESDDN 240
           NKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSSPDDESDDN
Sbjct: 181 NKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACVKRTSLQVSSPDDESDDN 240

Query: 241 SFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSALPFGHSKL 300
           SFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSALPFGHSKL
Sbjct: 241 SFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENLNNLTILNTSALPFGHSKL 300

Query: 301 DITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           DITIVAQVDVGFCLTVEDVVKNLRQALLDFH
Sbjct: 301 DITIVAQVDVGFCLTVEDVVKNLRQALLDFH 331

BLAST of Bhi03G000843 vs. NCBI nr
Match: XP_023004094.1 (transcription factor bHLH19-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 386.3 bits (991), Expect = 2.6e-103
Identity = 236/350 (67.43%), Postives = 262/350 (74.86%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +W+SEWE+ED P FMDEF+V PF+FS DE KFEE         DFY S  PPSA
Sbjct: 1   MEISSTKWVSEWEMEDAPYFMDEFQVHPFEFSFDEMKFEEN-----GRRDFYGSTDPPSA 60

Query: 61  VTERPTKQLKVAVDG-GIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG-----KGSVG 120
           V ER  KQL V V+G G   Q F    KADSSSS+S IISFE   N K G     KGSV 
Sbjct: 61  VAERVVKQLMVEVEGKGATSQGFAP--KADSSSSSSHIISFEKL-NCKAGKGCDVKGSVE 120

Query: 121 AKIEMDYE-------SVNISESYWNN--NEKSWKKCSSSMNRNPLHAREHVIAERKRREK 180
            KIEM YE       S  I+ES WNN  + +  +K + S NRNP HAREHV+AERKRREK
Sbjct: 121 PKIEMAYEYDGNLNFSTLITES-WNNEYSRQGVQKAAGSANRNPFHAREHVLAERKRREK 180

Query: 181 LSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPN-SKMSEAQSTACVK 240
           LSQRFIALSALIPGLNK+DKASILGGA+R+VKELQERLKVVEEQ   S++SE QST  VK
Sbjct: 181 LSQRFIALSALIPGLNKIDKASILGGAVRYVKELQERLKVVEEQAAISEVSEEQSTVYVK 240

Query: 241 RTSLQVSSPDDE---SDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 300
           R S   SS DD+   S++NSF+G P PEIEAR SNKDVLIRIHC KRKGCLSYLL KIEN
Sbjct: 241 RRSPVSSSDDDDTISSEENSFSGRPVPEIEARVSNKDVLIRIHCHKRKGCLSYLLGKIEN 300

Query: 301 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           L NLTIL++SALPF HSKLDITIVAQVD GFC+ VEDVVKNLRQALLDF+
Sbjct: 301 L-NLTILSSSALPFAHSKLDITIVAQVDDGFCMKVEDVVKNLRQALLDFN 340

BLAST of Bhi03G000843 vs. NCBI nr
Match: XP_023518686.1 (transcription factor bHLH19-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 371.3 bits (952), Expect = 8.6e-99
Identity = 233/350 (66.57%), Postives = 260/350 (74.29%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +W+SEWE+ED P FMDEF+V+PF+FS DE KFEE +G S    DFY S   PSA
Sbjct: 1   MEISSTKWVSEWEMEDAPYFMDEFQVNPFEFSFDEMKFEE-NGRS----DFYGSTDLPSA 60

Query: 61  VTERPTKQLKVAVDG-GIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG-----KGSVG 120
                     V V+G G   Q F    KADSSSS+S IISF+   N K G     KGSV 
Sbjct: 61  ----------VKVEGKGATSQGFAP--KADSSSSSSHIISFDKL-NCKAGKGCDVKGSVE 120

Query: 121 AKIEMDYE-------SVNISESYWNN--NEKSWKKCSSSMNRNPLHAREHVIAERKRREK 180
            KIEM YE       S  I+ES WNN  + +  KK + S+NRNP HAREHV+AERKRREK
Sbjct: 121 PKIEMAYESDGNLNFSTLITES-WNNEYSRQGAKKAAGSVNRNPFHAREHVLAERKRREK 180

Query: 181 LSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPN-SKMSEAQSTACVK 240
           LSQRFIALSALIPGLNK+DKASILGGA+R+VKELQERLKVVEEQ   SK+SE QST  VK
Sbjct: 181 LSQRFIALSALIPGLNKIDKASILGGAVRYVKELQERLKVVEEQAAISKVSEEQSTVYVK 240

Query: 241 RTSLQVSSPDDE---SDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 300
           R S   SS DD+   S++NSF+G P PEIEAR SNKDVLIRIHC KRKGCLSYLL KIEN
Sbjct: 241 RRSPASSSDDDDTISSEENSFSGRPVPEIEARVSNKDVLIRIHCHKRKGCLSYLLGKIEN 300

Query: 301 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           L NLTILN+SALPF HSKLDITIVAQVD GFC+ VEDVVKNLRQALLDF+
Sbjct: 301 L-NLTILNSSALPFAHSKLDITIVAQVDDGFCMKVEDVVKNLRQALLDFN 330

BLAST of Bhi03G000843 vs. NCBI nr
Match: XP_022963167.1 (transcription factor bHLH19-like [Cucurbita moschata])

HSP 1 Score: 368.6 bits (945), Expect = 5.6e-98
Identity = 232/350 (66.29%), Postives = 258/350 (73.71%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +W+SEWE+ED P FMDEF+V+PF+FS DE KFEE +G S    DFY S   PSA
Sbjct: 1   MEISSTKWVSEWEMEDAPYFMDEFQVNPFEFSFDEMKFEE-NGRS----DFYGSTDLPSA 60

Query: 61  VTERPTKQLKVAVDG-GIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG-----KGSVG 120
                     V V+G G   Q F    KADSSSS+S IISFE   N K G     KGSV 
Sbjct: 61  ----------VKVEGKGATSQGFAP--KADSSSSSSHIISFEKL-NCKAGKGCDVKGSVE 120

Query: 121 AKIEMDYE-------SVNISESYWNN--NEKSWKKCSSSMNRNPLHAREHVIAERKRREK 180
            KIEM YE       S  I+ES WNN  + +  KK + S+NRNP HAREHV+AERKRREK
Sbjct: 121 PKIEMAYESDRNLNFSTLITES-WNNEYSRQGAKKAAGSVNRNPFHAREHVLAERKRREK 180

Query: 181 LSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPN-SKMSEAQSTACVK 240
           LSQRFIALSALIPGLNK+DKASIL GA+R+VKELQERLKVVEEQ   SK+SE QST  VK
Sbjct: 181 LSQRFIALSALIPGLNKIDKASILSGAVRYVKELQERLKVVEEQAAISKVSEEQSTVYVK 240

Query: 241 RTSLQVSSPDDE---SDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 300
           R S   SS DD+   S++NSF+G P PEIEAR SNKDVL RIHC KRKGCLSYLL KIEN
Sbjct: 241 RRSPASSSDDDDTISSEENSFSGRPVPEIEARVSNKDVLFRIHCHKRKGCLSYLLGKIEN 300

Query: 301 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           L NLTILN+SALPF HSKLDITIVAQVD GFC+ VEDVVKNLRQALLDF+
Sbjct: 301 L-NLTILNSSALPFAHSKLDITIVAQVDDGFCMKVEDVVKNLRQALLDFN 330

BLAST of Bhi03G000843 vs. NCBI nr
Match: XP_023004095.1 (transcription factor bHLH19-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 368.2 bits (944), Expect = 7.3e-98
Identity = 230/350 (65.71%), Postives = 256/350 (73.14%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +W+SEWE+ED P FMDEF+V PF+FS DE KFEE         DFY S  PPSA
Sbjct: 1   MEISSTKWVSEWEMEDAPYFMDEFQVHPFEFSFDEMKFEEN-----GRRDFYGSTDPPSA 60

Query: 61  VTERPTKQLKVAVDG-GIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG-----KGSVG 120
                     V V+G G   Q F    KADSSSS+S IISFE   N K G     KGSV 
Sbjct: 61  ----------VEVEGKGATSQGFAP--KADSSSSSSHIISFEKL-NCKAGKGCDVKGSVE 120

Query: 121 AKIEMDYE-------SVNISESYWNN--NEKSWKKCSSSMNRNPLHAREHVIAERKRREK 180
            KIEM YE       S  I+ES WNN  + +  +K + S NRNP HAREHV+AERKRREK
Sbjct: 121 PKIEMAYEYDGNLNFSTLITES-WNNEYSRQGVQKAAGSANRNPFHAREHVLAERKRREK 180

Query: 181 LSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPN-SKMSEAQSTACVK 240
           LSQRFIALSALIPGLNK+DKASILGGA+R+VKELQERLKVVEEQ   S++SE QST  VK
Sbjct: 181 LSQRFIALSALIPGLNKIDKASILGGAVRYVKELQERLKVVEEQAAISEVSEEQSTVYVK 240

Query: 241 RTSLQVSSPDDE---SDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 300
           R S   SS DD+   S++NSF+G P PEIEAR SNKDVLIRIHC KRKGCLSYLL KIEN
Sbjct: 241 RRSPVSSSDDDDTISSEENSFSGRPVPEIEARVSNKDVLIRIHCHKRKGCLSYLLGKIEN 300

Query: 301 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           L NLTIL++SALPF HSKLDITIVAQVD GFC+ VEDVVKNLRQALLDF+
Sbjct: 301 L-NLTILSSSALPFAHSKLDITIVAQVDDGFCMKVEDVVKNLRQALLDFN 330

BLAST of Bhi03G000843 vs. ExPASy TrEMBL
Match: A0A6J1KV88 (transcription factor bHLH19-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497507 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.2e-103
Identity = 236/350 (67.43%), Postives = 262/350 (74.86%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +W+SEWE+ED P FMDEF+V PF+FS DE KFEE         DFY S  PPSA
Sbjct: 1   MEISSTKWVSEWEMEDAPYFMDEFQVHPFEFSFDEMKFEEN-----GRRDFYGSTDPPSA 60

Query: 61  VTERPTKQLKVAVDG-GIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG-----KGSVG 120
           V ER  KQL V V+G G   Q F    KADSSSS+S IISFE   N K G     KGSV 
Sbjct: 61  VAERVVKQLMVEVEGKGATSQGFAP--KADSSSSSSHIISFEKL-NCKAGKGCDVKGSVE 120

Query: 121 AKIEMDYE-------SVNISESYWNN--NEKSWKKCSSSMNRNPLHAREHVIAERKRREK 180
            KIEM YE       S  I+ES WNN  + +  +K + S NRNP HAREHV+AERKRREK
Sbjct: 121 PKIEMAYEYDGNLNFSTLITES-WNNEYSRQGVQKAAGSANRNPFHAREHVLAERKRREK 180

Query: 181 LSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPN-SKMSEAQSTACVK 240
           LSQRFIALSALIPGLNK+DKASILGGA+R+VKELQERLKVVEEQ   S++SE QST  VK
Sbjct: 181 LSQRFIALSALIPGLNKIDKASILGGAVRYVKELQERLKVVEEQAAISEVSEEQSTVYVK 240

Query: 241 RTSLQVSSPDDE---SDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 300
           R S   SS DD+   S++NSF+G P PEIEAR SNKDVLIRIHC KRKGCLSYLL KIEN
Sbjct: 241 RRSPVSSSDDDDTISSEENSFSGRPVPEIEARVSNKDVLIRIHCHKRKGCLSYLLGKIEN 300

Query: 301 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           L NLTIL++SALPF HSKLDITIVAQVD GFC+ VEDVVKNLRQALLDF+
Sbjct: 301 L-NLTILSSSALPFAHSKLDITIVAQVDDGFCMKVEDVVKNLRQALLDFN 340

BLAST of Bhi03G000843 vs. ExPASy TrEMBL
Match: A0A6J1HEG9 (transcription factor bHLH19-like OS=Cucurbita moschata OX=3662 GN=LOC111463455 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.7e-98
Identity = 232/350 (66.29%), Postives = 258/350 (73.71%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +W+SEWE+ED P FMDEF+V+PF+FS DE KFEE +G S    DFY S   PSA
Sbjct: 1   MEISSTKWVSEWEMEDAPYFMDEFQVNPFEFSFDEMKFEE-NGRS----DFYGSTDLPSA 60

Query: 61  VTERPTKQLKVAVDG-GIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG-----KGSVG 120
                     V V+G G   Q F    KADSSSS+S IISFE   N K G     KGSV 
Sbjct: 61  ----------VKVEGKGATSQGFAP--KADSSSSSSHIISFEKL-NCKAGKGCDVKGSVE 120

Query: 121 AKIEMDYE-------SVNISESYWNN--NEKSWKKCSSSMNRNPLHAREHVIAERKRREK 180
            KIEM YE       S  I+ES WNN  + +  KK + S+NRNP HAREHV+AERKRREK
Sbjct: 121 PKIEMAYESDRNLNFSTLITES-WNNEYSRQGAKKAAGSVNRNPFHAREHVLAERKRREK 180

Query: 181 LSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPN-SKMSEAQSTACVK 240
           LSQRFIALSALIPGLNK+DKASIL GA+R+VKELQERLKVVEEQ   SK+SE QST  VK
Sbjct: 181 LSQRFIALSALIPGLNKIDKASILSGAVRYVKELQERLKVVEEQAAISKVSEEQSTVYVK 240

Query: 241 RTSLQVSSPDDE---SDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 300
           R S   SS DD+   S++NSF+G P PEIEAR SNKDVL RIHC KRKGCLSYLL KIEN
Sbjct: 241 RRSPASSSDDDDTISSEENSFSGRPVPEIEARVSNKDVLFRIHCHKRKGCLSYLLGKIEN 300

Query: 301 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           L NLTILN+SALPF HSKLDITIVAQVD GFC+ VEDVVKNLRQALLDF+
Sbjct: 301 L-NLTILNSSALPFAHSKLDITIVAQVDDGFCMKVEDVVKNLRQALLDFN 330

BLAST of Bhi03G000843 vs. ExPASy TrEMBL
Match: A0A6J1KTM8 (transcription factor bHLH19-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497507 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 3.5e-98
Identity = 230/350 (65.71%), Postives = 256/350 (73.14%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +W+SEWE+ED P FMDEF+V PF+FS DE KFEE         DFY S  PPSA
Sbjct: 1   MEISSTKWVSEWEMEDAPYFMDEFQVHPFEFSFDEMKFEEN-----GRRDFYGSTDPPSA 60

Query: 61  VTERPTKQLKVAVDG-GIKCQIFDGNYKADSSSSTSQIISFENFSNSKCG-----KGSVG 120
                     V V+G G   Q F    KADSSSS+S IISFE   N K G     KGSV 
Sbjct: 61  ----------VEVEGKGATSQGFAP--KADSSSSSSHIISFEKL-NCKAGKGCDVKGSVE 120

Query: 121 AKIEMDYE-------SVNISESYWNN--NEKSWKKCSSSMNRNPLHAREHVIAERKRREK 180
            KIEM YE       S  I+ES WNN  + +  +K + S NRNP HAREHV+AERKRREK
Sbjct: 121 PKIEMAYEYDGNLNFSTLITES-WNNEYSRQGVQKAAGSANRNPFHAREHVLAERKRREK 180

Query: 181 LSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPN-SKMSEAQSTACVK 240
           LSQRFIALSALIPGLNK+DKASILGGA+R+VKELQERLKVVEEQ   S++SE QST  VK
Sbjct: 181 LSQRFIALSALIPGLNKIDKASILGGAVRYVKELQERLKVVEEQAAISEVSEEQSTVYVK 240

Query: 241 RTSLQVSSPDDE---SDDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 300
           R S   SS DD+   S++NSF+G P PEIEAR SNKDVLIRIHC KRKGCLSYLL KIEN
Sbjct: 241 RRSPVSSSDDDDTISSEENSFSGRPVPEIEARVSNKDVLIRIHCHKRKGCLSYLLGKIEN 300

Query: 301 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           L NLTIL++SALPF HSKLDITIVAQVD GFC+ VEDVVKNLRQALLDF+
Sbjct: 301 L-NLTILSSSALPFAHSKLDITIVAQVDDGFCMKVEDVVKNLRQALLDFN 330

BLAST of Bhi03G000843 vs. ExPASy TrEMBL
Match: A0A1S3B172 (transcription factor bHLH18-like OS=Cucumis melo OX=3656 GN=LOC103484895 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 6.0e-98
Identity = 226/349 (64.76%), Postives = 252/349 (72.21%), Query Frame = 0

Query: 1   MEISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGGSWSGTDFYESPVPPSA 60
           MEISS +WIS+WEIED   FMDEFK+DPF+FSLD  +F+          D YE PVPP A
Sbjct: 1   MEISSVKWISDWEIEDASPFMDEFKIDPFEFSLDALQFD----------DLYELPVPPYA 60

Query: 61  VTERPTKQLKV-------AVDGGI-KCQ--IFDGNYKA-DSS-SSTSQIISFENFSNSKC 120
           V E+P KQ+KV       A  GGI KC+      NYK  DSS SS+S+IISFEN      
Sbjct: 61  VEEKPAKQVKVEKEEEEQAGAGGINKCKTTFVGNNYKGRDSSLSSSSRIISFEN------ 120

Query: 121 GKGSVGAKIEMDYESVNISESYWNNNEKSWKKCSSSMNRNPLHAREHVIAERKRREKLSQ 180
                      ++ESVN     WN+N     K S SMNRN ++ REHVIAERKRREKLSQ
Sbjct: 121 ---------NWNHESVN----NWNSNN---GKRSCSMNRNAMNGREHVIAERKRREKLSQ 180

Query: 181 RFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQ-STACVKRTS 240
           RFIALSALIPGLNKVDKASILGGAIRHVKELQERLK VEEQ  SK SE Q    CVKRT+
Sbjct: 181 RFIALSALIPGLNKVDKASILGGAIRHVKELQERLKGVEEQTTSKTSEPQVPVVCVKRTT 240

Query: 241 LQVSSPDDE---SDDNSFTG--GPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIENL 300
           LQ SS DD+   SD+NSF+G     PEIEARF N DVLIRIHC KRKGCLSYLLN+I++L
Sbjct: 241 LQPSSSDDDTSSSDENSFSGRLRSTPEIEARFLNNDVLIRIHCHKRKGCLSYLLNRIQSL 300

Query: 301 NNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDFH 332
           NNLTILNT ALPF HS LDITIVAQ+DVGF +TVEDVVKNLRQALLDF+
Sbjct: 301 NNLTILNTCALPFSHSNLDITIVAQMDVGFHMTVEDVVKNLRQALLDFN 317

BLAST of Bhi03G000843 vs. ExPASy TrEMBL
Match: A0A6J1BY31 (transcription factor bHLH19-like OS=Momordica charantia OX=3673 GN=LOC111005773 PE=4 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 4.3e-96
Identity = 217/349 (62.18%), Postives = 256/349 (73.35%), Query Frame = 0

Query: 3   ISSARWISEWEIEDPPSFMDEFKVDPFDFSLDEFKFEEYSGG---SWSGTDFYESPVPPS 62
           ISSARWISEWE+E+  +F+DE ++DPF+FS DEFKFE+  G    + +  + Y+   P +
Sbjct: 4   ISSARWISEWEMEE-TAFIDEIQIDPFEFSFDEFKFEQSDGAGTVTATAAEIYDYG-PSA 63

Query: 63  AVTERPTKQLKVAVDGGIKCQIFDGNYKADSSSSTSQIISFENFSNSKCGKGSVGAKIEM 122
            V ERP KQLKVA   G  CQIF    KADSSSS+S II F++ SNSK     V  K+EM
Sbjct: 64  VVEERPAKQLKVAAADGAHCQIFAPTPKADSSSSSSHIICFQS-SNSK-SSTVVEPKLEM 123

Query: 123 DYESVN-------ISESYW-NNNEKSWKK--------CSSSMNRNPLHAREHVIAERKRR 182
           D+ES         ISES W  NN   W +         S SMNRNPLHAREHVIAERKRR
Sbjct: 124 DFESDRNLNFSSLISESSWKENNRYCWSRNGQGTKRAASGSMNRNPLHAREHVIAERKRR 183

Query: 183 EKLSQRFIALSALIPGLNKVDKASILGGAIRHVKELQERLKVVEEQPNSKMSEAQSTACV 242
           EKLSQRFIALSALIPGLNK+DKASILGGA+R+VKELQERLK  EE+  SK+SE  +T  V
Sbjct: 184 EKLSQRFIALSALIPGLNKIDKASILGGAVRYVKELQERLKEAEERA-SKISEEAATVYV 243

Query: 243 KRTSLQVSSPDDES--DDNSFTGGPAPEIEARFSNKDVLIRIHCQKRKGCLSYLLNKIEN 302
           KR+SL  S  D  S  ++   +GGP PEIEAR SNKDVL+RIH  KRKGCLSYL NKIEN
Sbjct: 244 KRSSLSCSDDDVSSSEENTDSSGGPVPEIEARVSNKDVLLRIHTHKRKGCLSYLHNKIEN 303

Query: 303 LNNLTILNTSALPFGHSKLDITIVAQVDVGFCLTVEDVVKNLRQALLDF 331
           L NLTILN++ALPF HS+L ITIVAQ+D+GFC+++EDVVKNLRQALL+F
Sbjct: 304 L-NLTILNSTALPFAHSQLHITIVAQMDIGFCMSMEDVVKNLRQALLEF 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G22770.14.3e-4036.66basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT4G37850.11.4e-3835.48basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G22750.22.4e-3840.59basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G22750.12.6e-3740.59basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G22760.11.9e-3545.88basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
Match NameE-valueIdentityDescription
Q8S3F16.1e-3936.66Transcription factor NAI1 OS=Arabidopsis thaliana OX=3702 GN=NAI1 PE=2 SV=1[more]
Q9T0722.0e-3735.48Transcription factor bHLH25 OS=Arabidopsis thaliana OX=3702 GN=BHLH25 PE=2 SV=2[more]
Q1PF173.3e-3740.59Transcription factor bHLH18 OS=Arabidopsis thaliana OX=3702 GN=BHLH18 PE=1 SV=1[more]
Q1PF162.6e-3445.88Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1[more]
Q9ZPY84.0e-1434.04Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
XP_038883438.11.5e-183100.00transcription factor bHLH18-like [Benincasa hispida][more]
XP_023004094.12.6e-10367.43transcription factor bHLH19-like isoform X1 [Cucurbita maxima][more]
XP_023518686.18.6e-9966.57transcription factor bHLH19-like [Cucurbita pepo subsp. pepo][more]
XP_022963167.15.6e-9866.29transcription factor bHLH19-like [Cucurbita moschata][more]
XP_023004095.17.3e-9865.71transcription factor bHLH19-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1KV881.2e-10367.43transcription factor bHLH19-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HEG92.7e-9866.29transcription factor bHLH19-like OS=Cucurbita moschata OX=3662 GN=LOC111463455 P... [more]
A0A6J1KTM83.5e-9865.71transcription factor bHLH19-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A1S3B1726.0e-9864.76transcription factor bHLH18-like OS=Cucumis melo OX=3656 GN=LOC103484895 PE=4 SV... [more]
A0A6J1BY314.3e-9662.18transcription factor bHLH19-like OS=Momordica charantia OX=3673 GN=LOC111005773 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 189..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 207..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..239
NoneNo IPR availablePANTHERPTHR45959:SF36TRANSCRIPTION FACTOR NAI1coord: 58..330
NoneNo IPR availablePANTHERPTHR45959BHLH TRANSCRIPTION FACTORcoord: 58..330
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 156..205
e-value: 8.9E-14
score: 61.8
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 154..200
e-value: 3.3E-9
score: 36.6
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 150..199
score: 14.779739
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 151..224
e-value: 1.8E-13
score: 52.4
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 155..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000843Bhi03M000843mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity