Bhi03G000666 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000666
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptioncytochrome P450 84A1-like
Locationchr3: 16740926 .. 16743209 (-)
RNA-Seq ExpressionBhi03G000666
SyntenyBhi03G000666
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTCAATATTCTCTTCTTCAACCTCTCAATATGCTTTTCTTTTTCACACTTCCTCTTCTGTTTCTTCTTACACTGCTTTTCCCACCTTTCCGGCGAAAACTACCCTTACCACCCGGCCCAAAAGGCCTCCCCTTCATCGGTAACATGCTGATGATGGATCAGCTCACCCATCGTGGACTCGCCCGTCTCGCCGCCCGCTACGGCGGAATCTTCCATATGCGCATGGGCTTCCTTCACATGGTTACCGTCTCCAACCCCGATATCGCACGCCAAGTTCTTCAGGTTCCTCTTATATTCAAAACTACTCTCAAACAAAATCTTGTCCTACACTTTTTTTTGTTCATTCAATTTGAGGTTGACAAACTTCTTTTTTTTAAAAAAAATTGTTATATATTTAATTATTAATTATTAGATTAATCACAATTATCTTTTTTAAAAATGAAAAAAAAAATCGAAGTGAAATGTTTTATTGATATATTTATTTATATTGTCAACTAGGTACATGATGGTATTTGTTCTAACCGTCCTGCCACCATTGCCATAGCTTACTTAACATACAACCGTGCCGACATGGCTTTCGCTCATTACGGCCCATTCTGGCGGCAGATGCGTAAGCTTTGCGTCATGAAATTATTCAGCCGGAAGCGAGCTGAATCGTGGGAGTCTGTTCGAGATGAGGTGGACAACACCATTGGAAAAGTTGCTGTCCGGACAGGAACCGCCGTGAACATCGGGGAGCTAGTGTTTGAGCTGACAAGAGATATTATTTATAGAGCCGCTTTTGGAACTAGCGCGGAGCAAGGTCAAGACGAGTTTATAAGAATCTTGCAAGAATTCTCTCAACTTTTCGGAGCTTTCAATATCACGGATTTTATTCCGATGCTCGGTTGGATTGACCCTCAAGGCTTAAACAACAGGCTTGTGAAGGCTCGTCGATCGCTCGATAAATTTATCGACTCCATAATCGACGATCACACAAATAGAAAAAAAACCACATCTAAATTACCAAATTACGTCAAAGACCATGAGATTGATAGTGATATGGTGGATGAGTTACTGGCTTTTTACAGTGAGGATTCAACAGCATCAAATACATCGGAGGATCTACAAAATAATTTCAAACTCAACCGTGACAACATCAAAGCTATCATCATGGTAATTAAAATTAGTTCAGTATCAAATATATTAACCTTTCATAAATCATAGTGATCATAGTCAACATTTACAAAATTGCCCCACTAGGAAAAAAGTTGGAAAAAATATTTACTTTTTTGGGCAATACATAATATGGCATTTAATCGAGTTTTATTTTGGGCAACTCTTTCATCTGAAGGAATCATATATATATATATATATTTTCTTTTCTACCCTTATATATATTTCTTCCCCCAATTTTACAGTTAATATTTTGACTTTTTCCCAAGAGTTCCTAGGCTTTAATTACTATAAATTTATTTTGTTTACACGTCACGTTTTTTGTTTTGTAGACTTACGTTTTTAGAAATTTCTGGGTTCTCTTGTGTAGCTTTCTTTTCTTGTTGGCCAAAGTTTTTAAGAATCTATTTTTCTTAACAGATTTTTTTTTTTTTTGGAAAAACCCCACAAAATAGGCTGTTGCTTCTTGGTGGTTGTGAAGAATAATATTTTGTTGTTAGCTCTGCTGCAACCATTTATTTTCTTTTTGACAATATTAAATATTCGGTAGAAAATTTTAAATATATATATATGAAAAAAGAAAATTGTGATTTGACAAATCAAGTTACTATTTTTCTTACTATTGTGTGAACCCGTTTTAATTTTGTTGGAAATTAAAATACAAACGGCAGGATGTTATGTTTGGTGGAACGGAGACGGTAGCGTCGGCGATAGAGTGGGCCATGTCGGAGCTAATGAGAAGCCCAGAAGATTTAAAGAAGGCCCAGCAAGAACTCGCCCAAGTCGTTGGTCTCGAACGACGTGTCGAAGAATCCGACTTCGAGAATTTGACCTTTTTCAAATGCTGCCTCAAAGAGACTCTTCGCCTGCACCCGCCGATCCCGCTCCTCCTCCACGAGACAGCGGAGGATGCGGTGGTCGCTGGCTACTTCATTCCGGCGAAATCTCGGGTCATGGTGAACGCGTGGGCCATCGGGAGGGATCCGGCGTCATGGGACGACGCCGAAACATTCCGGCCGGCGAGGTTTCTGGAACAGGGAGTCCCGGATTTTAAAGGGAACAATTTCGAGTTCATTCCGTTCGGGTCGGGTCGTCGATCTTGCCCCGGAATGCAATTGGGTTT

mRNA sequence

ATGGATTCTCAATATTCTCTTCTTCAACCTCTCAATATGCTTTTCTTTTTCACACTTCCTCTTCTGTTTCTTCTTACACTGCTTTTCCCACCTTTCCGGCGAAAACTACCCTTACCACCCGGCCCAAAAGGCCTCCCCTTCATCGGTAACATGCTGATGATGGATCAGCTCACCCATCGTGGACTCGCCCGTCTCGCCGCCCGCTACGGCGGAATCTTCCATATGCGCATGGGCTTCCTTCACATGGTTACCGTCTCCAACCCCGATATCGCACGCCAAGTTCTTCAGGTACATGATGGTATTTGTTCTAACCGTCCTGCCACCATTGCCATAGCTTACTTAACATACAACCGTGCCGACATGGCTTTCGCTCATTACGGCCCATTCTGGCGGCAGATGCGTAAGCTTTGCGTCATGAAATTATTCAGCCGGAAGCGAGCTGAATCGTGGGAGTCTGTTCGAGATGAGGTGGACAACACCATTGGAAAAGTTGCTGTCCGGACAGGAACCGCCGTGAACATCGGGGAGCTAGTGTTTGAGCTGACAAGAGATATTATTTATAGAGCCGCTTTTGGAACTAGCGCGGAGCAAGGTCAAGACGAGTTTATAAGAATCTTGCAAGAATTCTCTCAACTTTTCGGAGCTTTCAATATCACGGATTTTATTCCGATGCTCGGTTGGATTGACCCTCAAGGCTTAAACAACAGGCTTGTGAAGGCTCGTCGATCGCTCGATAAATTTATCGACTCCATAATCGACGATCACACAAATAGAAAAAAAACCACATCTAAATTACCAAATTACGTCAAAGACCATGAGATTGATAGTGATATGGTGGATGAGTTACTGGCTTTTTACAGTGAGGATTCAACAGCATCAAATACATCGGAGGATCTACAAAATAATTTCAAACTCAACCGTGACAACATCAAAGCTATCATCATGGATGTTATGTTTGGTGGAACGGAGACGGTAGCGTCGGCGATAGAGTGGGCCATGTCGGAGCTAATGAGAAGCCCAGAAGATTTAAAGAAGGCCCAGCAAGAACTCGCCCAAGTCGTTGGTCTCGAACGACGTGTCGAAGAATCCGACTTCGAGAATTTGACCTTTTTCAAATGCTGCCTCAAAGAGACTCTTCGCCTGCACCCGCCGATCCCGCTCCTCCTCCACGAGACAGCGGAGGATGCGGTGGTCGCTGGCTACTTCATTCCGGCGAAATCTCGGGTCATGGTGAACGCGTGGGCCATCGGGAGGGATCCGGCGTCATGGGACGACGCCGAAACATTCCGGCCGGCGAGGTTTCTGGAACAGGGAGTCCCGGATTTTAAAGGGAACAATTTCGAGTTCATTCCGTTCGGGTCGGGTCGTCGATCTTGCCCCGGAATGCAATTGGGTTT

Coding sequence (CDS)

ATGGATTCTCAATATTCTCTTCTTCAACCTCTCAATATGCTTTTCTTTTTCACACTTCCTCTTCTGTTTCTTCTTACACTGCTTTTCCCACCTTTCCGGCGAAAACTACCCTTACCACCCGGCCCAAAAGGCCTCCCCTTCATCGGTAACATGCTGATGATGGATCAGCTCACCCATCGTGGACTCGCCCGTCTCGCCGCCCGCTACGGCGGAATCTTCCATATGCGCATGGGCTTCCTTCACATGGTTACCGTCTCCAACCCCGATATCGCACGCCAAGTTCTTCAGGTACATGATGGTATTTGTTCTAACCGTCCTGCCACCATTGCCATAGCTTACTTAACATACAACCGTGCCGACATGGCTTTCGCTCATTACGGCCCATTCTGGCGGCAGATGCGTAAGCTTTGCGTCATGAAATTATTCAGCCGGAAGCGAGCTGAATCGTGGGAGTCTGTTCGAGATGAGGTGGACAACACCATTGGAAAAGTTGCTGTCCGGACAGGAACCGCCGTGAACATCGGGGAGCTAGTGTTTGAGCTGACAAGAGATATTATTTATAGAGCCGCTTTTGGAACTAGCGCGGAGCAAGGTCAAGACGAGTTTATAAGAATCTTGCAAGAATTCTCTCAACTTTTCGGAGCTTTCAATATCACGGATTTTATTCCGATGCTCGGTTGGATTGACCCTCAAGGCTTAAACAACAGGCTTGTGAAGGCTCGTCGATCGCTCGATAAATTTATCGACTCCATAATCGACGATCACACAAATAGAAAAAAAACCACATCTAAATTACCAAATTACGTCAAAGACCATGAGATTGATAGTGATATGGTGGATGAGTTACTGGCTTTTTACAGTGAGGATTCAACAGCATCAAATACATCGGAGGATCTACAAAATAATTTCAAACTCAACCGTGACAACATCAAAGCTATCATCATGGATGTTATGTTTGGTGGAACGGAGACGGTAGCGTCGGCGATAGAGTGGGCCATGTCGGAGCTAATGAGAAGCCCAGAAGATTTAAAGAAGGCCCAGCAAGAACTCGCCCAAGTCGTTGGTCTCGAACGACGTGTCGAAGAATCCGACTTCGAGAATTTGACCTTTTTCAAATGCTGCCTCAAAGAGACTCTTCGCCTGCACCCGCCGATCCCGCTCCTCCTCCACGAGACAGCGGAGGATGCGGTGGTCGCTGGCTACTTCATTCCGGCGAAATCTCGGGTCATGGTGAACGCGTGGGCCATCGGGAGGGATCCGGCGTCATGGGACGACGCCGAAACATTCCGGCCGGCGAGGTTTCTGGAACAGGGAGTCCCGGATTTTAAAGGGAACAATTTCGAGTTCATTCCGTTCGGGTCGGGTCGTCGATCTTGCCCCGGAATGCAATTGGGTTT

Protein sequence

MDSQYSLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKARRSLDKFIDSIIDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG
Homology
BLAST of Bhi03G000666 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 653.7 bits (1685), Expect = 1.1e-187
Identity = 324/465 (69.68%), Postives = 382/465 (82.15%), Query Frame = 0

Query: 6   SLLQPLNMLFFFTLPLLFLLTLL----FPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRG 65
           S+ Q L+ L   T  L+ +++L     F   RR+ P PPGP+G P IGNMLMMDQLTHRG
Sbjct: 4   SISQTLSKLSDPTTSLVIVVSLFIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRG 63

Query: 66  LARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADM 125
           LA LA +YGG+ H+RMGFLHM  VS+P++ARQVLQV D + SNRPATIAI+YLTY+RADM
Sbjct: 64  LANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADM 123

Query: 126 AFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFEL 185
           AFAHYGPFWRQMRK+CVMK+FSRKRAESW SVRDEVD  +  V+   G  +N+GE +F L
Sbjct: 124 AFAHYGPFWRQMRKVCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGKPINVGEQIFAL 183

Query: 186 TRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKAR 245
           TR+I YRAAFG++ E+GQDEFIRILQEFS+LFGAFN+ DFIP  GWIDPQG+N RLVKAR
Sbjct: 184 TRNITYRAAFGSACEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKAR 243

Query: 246 RSLDKFIDSIIDDHTNRKKTTSKLPNYVKDHE-IDSDMVDELLAFYSEDSTASNTSEDLQ 305
             LD FID IID+H  +K+      N V D + +D+DMVD+LLAFYSE++   + + DLQ
Sbjct: 244 NDLDGFIDDIIDEHMKKKEN----QNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQ 303

Query: 306 NNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRV 365
           N+ KL RDNIKAIIMDVMFGGTETVASAIEWA++EL+RSPEDLK+ QQELA+VVGL+RRV
Sbjct: 304 NSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRV 363

Query: 366 EESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDP 425
           EESD E LT+ KC LKETLR+HPPIPLLLHETAED  + G+FIP KSRVM+NA+AIGRDP
Sbjct: 364 EESDIEKLTYLKCTLKETLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMINAFAIGRDP 423

Query: 426 ASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
            SW D +TFRP+RFLE GVPDFKG+NFEFIPFGSGRRSCPGMQLG
Sbjct: 424 TSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLG 464

BLAST of Bhi03G000666 vs. TAIR 10
Match: AT5G04330.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 535.4 bits (1378), Expect = 4.5e-152
Identity = 280/468 (59.83%), Postives = 344/468 (73.50%), Query Frame = 0

Query: 13  MLFFFTLPLLF-LLTLLFPP--FRRKL---PLPPGPKGLPFIGNMLMMDQLTHRGLARLA 72
           ML   TL +L  LL  LFP    RR++   P PPGPKGLP IGN+LMM+Q  HRGLA+L+
Sbjct: 1   MLTLMTLIVLVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFNHRGLAKLS 60

Query: 73  ARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHY 132
             YGG+ H+R+GF H+  VS+PDIARQVLQV D + SNRP TIAI YLTY  +D+AF +Y
Sbjct: 61  RIYGGLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCNY 120

Query: 133 GPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDII 192
           GPFWR+MRKL VM LFSRKRAESW SV +EV  ++  VA   G  +NI +L F L+RDI 
Sbjct: 121 GPFWRRMRKLYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGKPLNICKLAFSLSRDIT 180

Query: 193 YRAAFG----TSAEQGQDEFIRILQEFSQLFGAFNITDFIP-MLGWIDPQGLNNRLVKAR 252
           +RAAFG    TS E   DEF+ I+QEFS+LFG FN+ D++P  L WIDPQG+N R+ KAR
Sbjct: 181 FRAAFGSSSSTSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVEKAR 240

Query: 253 RSLDKFIDSIIDDHTNRKKTTSKLPNYVKDH---EIDSDMVDELLAFYSEDSTASNTSED 312
           +SLD FI+S+IDDH ++KK         ++H   + ++DMVD+LLAFY E+   +N+   
Sbjct: 241 KSLDGFIESVIDDHLHKKK---------REHDNVDEETDMVDQLLAFYEEEVKVNNSVT- 300

Query: 313 LQNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLER 372
                K+N DNIK IIMDVMFGGTETVA AIEW ++E++RSPE++K+ Q EL  VVGL+R
Sbjct: 301 -----KINLDNIKGIIMDVMFGGTETVALAIEWVLTEILRSPENMKRVQDELTSVVGLDR 360

Query: 373 -RVEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIG 432
            RVE++  E LTF KC LKETLRLHPP PLLLHET +D  ++GYFIP  SRVMVN +A+G
Sbjct: 361 WRVEDTHLEKLTFLKCILKETLRLHPPFPLLLHETVKDTEISGYFIPKGSRVMVNTYALG 420

Query: 433 RDPASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           RDP SW D E+F P RFL    PD KGNNFEF+PFGSGRRSCPGMQLG
Sbjct: 421 RDPNSWSDPESFNPGRFLNPIAPDLKGNNFEFVPFGSGRRSCPGMQLG 453

BLAST of Bhi03G000666 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 308.9 bits (790), Expect = 6.8e-84
Identity = 180/469 (38.38%), Postives = 260/469 (55.44%), Query Frame = 0

Query: 13  MLFFFTLPLLFLLTLLFPPFR-------RKLPLPPGPKGLPFIGNMLMMDQLTHRGLARL 72
           M   + LPL+FL+ +L   F        R+ P PP   G P IGN+  + +L H+ L +L
Sbjct: 1   MAHIWLLPLIFLVCILLAVFNHKKHPKYRQFPCPP---GFPIIGNLHQIGELPHQTLWKL 60

Query: 73  AARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAH 132
           + +YG + H+ +G +  V VS+ D ARQVL+VHD  C  RP+      L+YN  D+AF+ 
Sbjct: 61  SKKYGPVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSP 120

Query: 133 YGPFWRQMRKLCVMKLFSRKRAESWESVRD-EVDNTIGKVAVRTG--TAVNIGELVFELT 192
           Y  +W+++RKLCV +LFS K+  S + ++D EV   I  +A        VN+     ELT
Sbjct: 121 YDDYWKEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELT 180

Query: 193 RDIIYRAAFGTSAE---QGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDP--QGLNNRL 252
             ++ R AFG S E      D F +I++E  ++ G+F+  DFIP +GWI     GL  R 
Sbjct: 181 VSVVCRTAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRR 240

Query: 253 VKARRSLDKFIDSIIDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSE 312
            +++R L+ F + + D H   KK            E + D VD LL    E++   N   
Sbjct: 241 ERSKRDLNAFFEQMFDLHKEGKK------------EGNEDFVDLLLRLEKEEAVLGND-- 300

Query: 313 DLQNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLE 372
                 KL R++IKAI++DV+  G +T A  + WAM+EL R+P  +KK Q E+   +G  
Sbjct: 301 ------KLTRNHIKAILLDVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNR 360

Query: 373 RRVEESDFENLTFFKCCLKETLRLHPPIPLLL-HETAEDAVVAGYFIPAKSRVMVNAWAI 432
             +   D + L + K  +KET RLHP  PLLL  E   +  + GY IP K+R+ VN WAI
Sbjct: 361 SMISFEDMDQLEYLKMVIKETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAI 420

Query: 433 GRDPASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           GRDP +W D E F P RF++  + D KG +FE +PFG GRR CP + +G
Sbjct: 421 GRDPDTWKDPEVFLPERFMDNNI-DAKGQHFELLPFGGGRRICPAIYMG 445

BLAST of Bhi03G000666 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 306.2 bits (783), Expect = 4.4e-83
Identity = 166/459 (36.17%), Postives = 271/459 (59.04%), Query Frame = 0

Query: 13  MLFFFTLPLLFLLTLLFPPFR-----RKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAA 72
           ++FF    ++F++T++   FR     +K    P P GLP IGN+  + +  HR L  L+ 
Sbjct: 3   IMFFLLCSIIFVVTIII--FRKQKRGKKRNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSH 62

Query: 73  RYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYG 132
           RYG +  +  G + ++ VS+ ++AR VL+ HD + ++RP +     L Y++ D+A A YG
Sbjct: 63  RYGPLMLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYG 122

Query: 133 PFWRQMRKLCVMKLFSRKRAESWESVR-DEVDNTIGKVAVRTGTAVNIGELVFELTRDII 192
            +WRQM+ +CV+ LFS K   S+  VR +E+   + K+       VN+ +++  LT D+I
Sbjct: 123 EYWRQMKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSLTNDVI 182

Query: 193 YRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWID-PQGLNNRLVKARRSLD 252
            + A G     G+ +F  +++  ++L G F++  ++P L WID  +GL+ +L K    +D
Sbjct: 183 CKVALGRK-YGGETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVD 242

Query: 253 KFIDSIIDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKL 312
           KF + ++ DH +  +               +D VD LLA             D    F++
Sbjct: 243 KFFERVVQDHVDGNRDM-------------TDFVDVLLAI----------QRDKTVGFEI 302

Query: 313 NRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDF 372
           NR +IKAI+M+V  GGT+T ++ +EWAM+EL+R P+ LK+ Q+E+  +   +  V E + 
Sbjct: 303 NRVSIKAIVMNVFVGGTDTSSTLMEWAMTELLRHPKCLKRLQEEVRTICKDKSSVSEEEI 362

Query: 373 ENLTFFKCCLKETLRLHPPIPLLL-HETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASW- 432
           +N+++ K  +KE LRLHPP+PL++ HE+ +D  +  + IPA ++V++NAWAIGR+ A+W 
Sbjct: 363 QNMSYLKAVIKEALRLHPPLPLMVPHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWG 422

Query: 433 DDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGM 463
            D E FRP R L+  V D++G  FE IPFGSGRR CP +
Sbjct: 423 PDVEEFRPERHLDSSV-DYRGQAFELIPFGSGRRICPAI 434

BLAST of Bhi03G000666 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 9.9e-83
Identity = 174/458 (37.99%), Postives = 254/458 (55.46%), Query Frame = 0

Query: 21  LLFLLTLLFPPFRRKL---PLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGIFHMRM 80
           +LFL+  +F   R +     LPPGP   P IGN+  M    HR L+ +   YG I H+R+
Sbjct: 13  VLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRL 72

Query: 81  GFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQMRKLC 140
           GF+ +V  ++  +A Q L++HD   ++RP      ++ YN  D+ FA YG  WR +RK+ 
Sbjct: 73  GFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKIS 132

Query: 141 VMKLFSRKRAESWESVRDEVDNTIGKVAVRTGT-AVNIGEL-----VFELTRDIIYRAAF 200
            + LFS K  E ++ VR E   T+ +  VR GT  VN+G+L     V  L R++I R  F
Sbjct: 133 SVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREMIGRRLF 192

Query: 201 GTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKARRSLDKFIDSI 260
           G  A+   DEF  ++ E   L G FNI DF+P L W+D QG+  ++ +  +  D F+ SI
Sbjct: 193 GADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLSSI 252

Query: 261 IDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIK 320
           + +H        ++    + H   +DM+  L++    D      S        L    IK
Sbjct: 253 LKEH--------EMNGQDQKH---TDMLSTLISLKGTDLDGDGGS--------LTDTEIK 312

Query: 321 AIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFF 380
           A+++++   GT+T AS ++WA++EL+R P+ + KAQ+EL  VVG +R V ESD   L + 
Sbjct: 313 ALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYL 372

Query: 381 KCCLKETLRLHPPIPLLL-HETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFR 440
           +  +KE  RLHPP PL L H  +E   + GY IP  S ++ N WAI RDP  W D   F+
Sbjct: 373 QAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFK 432

Query: 441 PARFL---EQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           P RFL   E+   D KG++FE IPFG+GRR C G+ LG
Sbjct: 433 PERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLG 451

BLAST of Bhi03G000666 vs. ExPASy Swiss-Prot
Match: Q42600 (Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 1.6e-186
Identity = 324/465 (69.68%), Postives = 382/465 (82.15%), Query Frame = 0

Query: 6   SLLQPLNMLFFFTLPLLFLLTLL----FPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRG 65
           S+ Q L+ L   T  L+ +++L     F   RR+ P PPGP+G P IGNMLMMDQLTHRG
Sbjct: 4   SISQTLSKLSDPTTSLVIVVSLFIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRG 63

Query: 66  LARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADM 125
           LA LA +YGG+ H+RMGFLHM  VS+P++ARQVLQV D + SNRPATIAI+YLTY+RADM
Sbjct: 64  LANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADM 123

Query: 126 AFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFEL 185
           AFAHYGPFWRQMRK+CVMK+FSRKRAESW SVRDEVD  +  V+   G  +N+GE +F L
Sbjct: 124 AFAHYGPFWRQMRKVCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGKPINVGEQIFAL 183

Query: 186 TRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKAR 245
           TR+I YRAAFG++ E+GQDEFIRILQEFS+LFGAFN+ DFIP  GWIDPQG+N RLVKAR
Sbjct: 184 TRNITYRAAFGSACEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKAR 243

Query: 246 RSLDKFIDSIIDDHTNRKKTTSKLPNYVKDHE-IDSDMVDELLAFYSEDSTASNTSEDLQ 305
             LD FID IID+H  +K+      N V D + +D+DMVD+LLAFYSE++   + + DLQ
Sbjct: 244 NDLDGFIDDIIDEHMKKKEN----QNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQ 303

Query: 306 NNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRV 365
           N+ KL RDNIKAIIMDVMFGGTETVASAIEWA++EL+RSPEDLK+ QQELA+VVGL+RRV
Sbjct: 304 NSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRV 363

Query: 366 EESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDP 425
           EESD E LT+ KC LKETLR+HPPIPLLLHETAED  + G+FIP KSRVM+NA+AIGRDP
Sbjct: 364 EESDIEKLTYLKCTLKETLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMINAFAIGRDP 423

Query: 426 ASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
            SW D +TFRP+RFLE GVPDFKG+NFEFIPFGSGRRSCPGMQLG
Sbjct: 424 TSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLG 464

BLAST of Bhi03G000666 vs. ExPASy Swiss-Prot
Match: F4JW83 (Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 6.3e-151
Identity = 280/468 (59.83%), Postives = 344/468 (73.50%), Query Frame = 0

Query: 13  MLFFFTLPLLF-LLTLLFPP--FRRKL---PLPPGPKGLPFIGNMLMMDQLTHRGLARLA 72
           ML   TL +L  LL  LFP    RR++   P PPGPKGLP IGN+LMM+Q  HRGLA+L+
Sbjct: 1   MLTLMTLIVLVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFNHRGLAKLS 60

Query: 73  ARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHY 132
             YGG+ H+R+GF H+  VS+PDIARQVLQV D + SNRP TIAI YLTY  +D+AF +Y
Sbjct: 61  RIYGGLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCNY 120

Query: 133 GPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDII 192
           GPFWR+MRKL VM LFSRKRAESW SV +EV  ++  VA   G  +NI +L F L+RDI 
Sbjct: 121 GPFWRRMRKLYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGKPLNICKLAFSLSRDIT 180

Query: 193 YRAAFG----TSAEQGQDEFIRILQEFSQLFGAFNITDFIP-MLGWIDPQGLNNRLVKAR 252
           +RAAFG    TS E   DEF+ I+QEFS+LFG FN+ D++P  L WIDPQG+N R+ KAR
Sbjct: 181 FRAAFGSSSSTSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVEKAR 240

Query: 253 RSLDKFIDSIIDDHTNRKKTTSKLPNYVKDH---EIDSDMVDELLAFYSEDSTASNTSED 312
           +SLD FI+S+IDDH ++KK         ++H   + ++DMVD+LLAFY E+   +N+   
Sbjct: 241 KSLDGFIESVIDDHLHKKK---------REHDNVDEETDMVDQLLAFYEEEVKVNNSVT- 300

Query: 313 LQNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLER 372
                K+N DNIK IIMDVMFGGTETVA AIEW ++E++RSPE++K+ Q EL  VVGL+R
Sbjct: 301 -----KINLDNIKGIIMDVMFGGTETVALAIEWVLTEILRSPENMKRVQDELTSVVGLDR 360

Query: 373 -RVEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIG 432
            RVE++  E LTF KC LKETLRLHPP PLLLHET +D  ++GYFIP  SRVMVN +A+G
Sbjct: 361 WRVEDTHLEKLTFLKCILKETLRLHPPFPLLLHETVKDTEISGYFIPKGSRVMVNTYALG 420

Query: 433 RDPASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           RDP SW D E+F P RFL    PD KGNNFEF+PFGSGRRSCPGMQLG
Sbjct: 421 RDPNSWSDPESFNPGRFLNPIAPDLKGNNFEFVPFGSGRRSCPGMQLG 453

BLAST of Bhi03G000666 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 347.1 bits (889), Expect = 3.2e-94
Identity = 192/463 (41.47%), Postives = 274/463 (59.18%), Query Frame = 0

Query: 14  LFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGIF 73
           L F  + L F L  L     +K  LPP P  LP IGN+  +  L HR L  LA   G + 
Sbjct: 7   LLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLI 66

Query: 74  HMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQM 133
            + +G +  + VS  +IA ++L+ HD I ++RP+T A   + Y+  D+AF+ YG +WRQ+
Sbjct: 67  LLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQV 126

Query: 134 RKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVR------TGTAVNIGELVFELTRDIIY 193
           RK+CV++L S KR  S+ S+R+E    +G +  R      TG AVN+ EL+  L+   I 
Sbjct: 127 RKICVLELLSIKRVNSYRSIREE---EVGLMMERISQSCSTGEAVNLSELLLLLSSGTIT 186

Query: 194 RAAFG---TSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDP-QGLNNRLVKARRS 253
           R AFG      E+ +++F  +  E + L GAF + D+ P   W+D   G++ RL +    
Sbjct: 187 RVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGE 246

Query: 254 LDKFIDSIIDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNF 313
           LD F+D +IDDH   +K          D     D+VD LL    + S   +         
Sbjct: 247 LDAFVDHVIDDHLLSRKANG------SDGVEQKDLVDVLLHLQKDSSLGVH--------- 306

Query: 314 KLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEES 373
            LNR+N+KA+I+D+  GGT+T A  +EWAM+EL++ P+ ++KAQQE+ +VVG + +VEE 
Sbjct: 307 -LNRNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEE 366

Query: 374 DFENLTFFKCCLKETLRLHPPIPLLL-HETAEDAVVAGYFIPAKSRVMVNAWAIGRDPAS 433
           D   L + K  +KETLRLHP  PLL+  E+  D V+ GY IPAK+RV +NAWAIGRDP S
Sbjct: 367 DLHQLHYLKLIIKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKS 426

Query: 434 WDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           W++AE F P RF+   V DFKG +F+ IPFG+GRR CPG+  G
Sbjct: 427 WENAEEFLPERFVNNSV-DFKGQDFQLIPFGAGRRGCPGIAFG 449

BLAST of Bhi03G000666 vs. ExPASy Swiss-Prot
Match: A0A0N9HTU1 (Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 6.9e-89
Identity = 182/455 (40.00%), Postives = 265/455 (58.24%), Query Frame = 0

Query: 18  TLPLLFLLT--LLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGIFHM 77
           TL LLF+ T  +L   F  K  LPP P  LP +GN   +  L HR +  LAA+YG +  +
Sbjct: 8   TLFLLFISTVFILKRKFSHKPNLPPSPPKLPILGNFHQLGTLVHRAVTVLAAKYGPLMLL 67

Query: 78  RMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQMRK 137
             G   ++ VS+ + A+++++ HD   +NRP T A   L Y+  D++FA YG +WR+M+K
Sbjct: 68  HFGKTPVLIVSSQETAKEIMKTHDLALANRPLTTAARALLYDCTDISFAPYGEYWREMKK 127

Query: 138 LCVMKLFSRKRAESWESVRDEVDNTIGKVAVR---TGTAVNIGELVFELTRDIIYRAAFG 197
           + V+ L S K+ +S+ SVR+E+ + + K   R   TG  V++  +++  + D+++R   G
Sbjct: 128 MAVLNLLSIKKIQSFRSVREELASDMIKEITRLSKTGAPVDVTNMLYHFSEDLLFRCTLG 187

Query: 198 TSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDP-QGLNNRLVKARRSLDKFIDSI 257
               +GQ +F ++ ++F  L GAF   DF P + W+D   GLN +L K  R LD F+D +
Sbjct: 188 FK-PKGQHKFQKLSRDFLDLVGAFCFNDFFPGMAWMDALTGLNRKLKKGSRELDDFVDEL 247

Query: 258 IDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIK 317
           I++              VKD    ++ +D LL          +T  D     KL RDN+K
Sbjct: 248 IEERI----------AMVKDGVEPNEFLDLLL----------HTHRDTTQEIKLTRDNVK 307

Query: 318 AIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFF 377
           AII+D   GG +  AS +EWAM+ELMR+P  +K AQ+E+ +VVG + +V+E D   + F 
Sbjct: 308 AIILDTFLGGIDLPASVMEWAMAELMRNPSKMKIAQEEVRKVVGNKNKVDEDDVYQMNFL 367

Query: 378 KCCLKETLRLHPPIPLLL-HETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFR 437
           K  +KETLRLHPP PLL   E+     V  Y IP  + VM+N W I RDP  WD AE F 
Sbjct: 368 KSAVKETLRLHPPAPLLFARESYTSINVENYIIPPYTSVMINIWHIQRDPKLWDKAEEFI 427

Query: 438 PARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           P RF+  G+ D+K +++EFIPFGSGRR CPGM  G
Sbjct: 428 PERFMNSGI-DYKSHDYEFIPFGSGRRGCPGMSFG 440

BLAST of Bhi03G000666 vs. ExPASy Swiss-Prot
Match: A6YIH8 (Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.8e-87
Identity = 182/454 (40.09%), Postives = 267/454 (58.81%), Query Frame = 0

Query: 16  FFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNML-MMDQLTHRGLARLAARYGGIFH 75
           F  L  LFLL        +   LPPGP  LP +G+ML M+  L H  L  LA +YG + H
Sbjct: 10  FLFLSFLFLLRKWKNSNSQSKKLPPGPWKLPLLGSMLHMVGGLPHHVLRDLAKKYGPLMH 69

Query: 76  MRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQMR 135
           +++G +  V V++PD+A++VL+ HD   ++RP  +A   + YNR+D+AF  YG +WRQMR
Sbjct: 70  LQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLLAPEIVCYNRSDIAFCPYGDYWRQMR 129

Query: 136 KLCVMKLFSRKRAESWESV-RDEVDNTIGKVAVRTGTAVNIGELVFELTRDIIYRAAFGT 195
           K+CV+++ S K   S+ S+ RDEV   +  V   T   VN  E +F  T  +  R+AFG 
Sbjct: 130 KICVLEVLSAKNVRSFSSIRRDEVLRLVNFVRSSTSEPVNFTERLFLFTSSMTCRSAFGK 189

Query: 196 SAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDP-QGLNNRLVKARRSLDKFIDSII 255
             ++ Q+ FI++++E   L G F++ D  P L ++    G+  +++KA   +D  ++ +I
Sbjct: 190 VFKE-QETFIQLIKEVIGLAGGFDVADIFPSLKFLHVLTGMEGKIMKAHHKVDAIVEDVI 249

Query: 256 DDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIKA 315
           ++H  +K       N     E   D++D LL   ++              F +  DNIKA
Sbjct: 250 NEH--KKNLAMGKTNGALGGE---DLIDVLLRLMNDGGL----------QFPITNDNIKA 309

Query: 316 IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFFK 375
           II D+   GTET +S + WAM ++MR+P  L KAQ E+ +    +   +E+D E L + K
Sbjct: 310 IIFDMFAAGTETSSSTLVWAMVQMMRNPTILAKAQAEVREAFKGKETFDENDVEELKYLK 369

Query: 376 CCLKETLRLHPPIPLLL-HETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFRP 435
             +KETLRLHPP+PLL+  E  E+  + GY IP K++VMVN WA+GRDP  WDDA+ F+P
Sbjct: 370 LVIKETLRLHPPVPLLVPRECREETEINGYTIPVKTKVMVNVWALGRDPKYWDDADNFKP 429

Query: 436 ARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
            RF EQ   DF GNNFE++PFG GRR CPG+  G
Sbjct: 430 ERF-EQCSVDFIGNNFEYLPFGGGRRICPGISFG 446

BLAST of Bhi03G000666 vs. NCBI nr
Match: XP_038883545.1 (cytochrome P450 84A1-like [Benincasa hispida])

HSP 1 Score: 933.7 bits (2412), Expect = 6.1e-268
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MDSQYSLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHR 60
           MDSQYSLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHR
Sbjct: 1   MDSQYSLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHR 60

Query: 61  GLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRAD 120
           GLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRAD
Sbjct: 61  GLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRAD 120

Query: 121 MAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFE 180
           MAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFE
Sbjct: 121 MAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFE 180

Query: 181 LTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKA 240
           LTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKA
Sbjct: 181 LTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKA 240

Query: 241 RRSLDKFIDSIIDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQ 300
           RRSLDKFIDSIIDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQ
Sbjct: 241 RRSLDKFIDSIIDDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQ 300

Query: 301 NNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRV 360
           NNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRV
Sbjct: 301 NNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRV 360

Query: 361 EESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDP 420
           EESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDP
Sbjct: 361 EESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDP 420

Query: 421 ASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           ASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG
Sbjct: 421 ASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 465

BLAST of Bhi03G000666 vs. NCBI nr
Match: XP_008440792.1 (PREDICTED: cytochrome P450 84A1-like [Cucumis melo] >KAA0025694.1 cytochrome P450 84A1-like [Cucumis melo var. makuwa] >TYK12569.1 cytochrome P450 84A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 783.5 bits (2022), Expect = 1.0e-222
Identity = 396/466 (84.98%), Postives = 422/466 (90.56%), Query Frame = 0

Query: 1   MDSQYSLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHR 60
           MDSQ SLLQPLNM   FTL  LFLLTLLF   R KLP PPGPKGLPFIGNMLMM+QLTHR
Sbjct: 1   MDSQ-SLLQPLNMAVPFTLSFLFLLTLLFRRLRSKLPFPPGPKGLPFIGNMLMMEQLTHR 60

Query: 61  GLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRAD 120
           GLA LAARYGGIF+MRMGF++M  VSNPDIARQVLQVHD ICSNRPATIAI+YLTYN AD
Sbjct: 61  GLANLAARYGGIFYMRMGFVNMFVVSNPDIARQVLQVHDSICSNRPATIAISYLTYNCAD 120

Query: 121 MAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFE 180
           MAFA+YGPFWRQMRK+CVMKLFSRKRAESW+SVRD VDNT+ KVAV+TGTAVNIGELVFE
Sbjct: 121 MAFANYGPFWRQMRKICVMKLFSRKRAESWQSVRDMVDNTVKKVAVQTGTAVNIGELVFE 180

Query: 181 LTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKA 240
           L  DIIYRAAFGTS  QGQDEFIRILQEFS+LFGAFNI DFIP L WIDPQGLN RLVKA
Sbjct: 181 LMGDIIYRAAFGTSTLQGQDEFIRILQEFSKLFGAFNIADFIPSLRWIDPQGLNKRLVKA 240

Query: 241 RRSLDKFIDSIIDDHTNRKK-TTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDL 300
           R+SLDKFIDSIIDDH  RK+  +SKLPNY  + E +SDMVDELLAFY EDS+  N S+DL
Sbjct: 241 RQSLDKFIDSIIDDHIIRKRNASSKLPNY--ELETESDMVDELLAFYGEDSSIRNASDDL 300

Query: 301 QNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERR 360
           +NN KLNRDNIK IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQEL+QVVGL+RR
Sbjct: 301 KNNIKLNRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQRR 360

Query: 361 VEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRD 420
           VEE+D +NLTF KCCLKET+RLHPPIPLLLHE AEDAV+AGYFIPAKSR+MVNAWAIGRD
Sbjct: 361 VEETDLDNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGRD 420

Query: 421 PASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           PASWDDAETFRP RFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG
Sbjct: 421 PASWDDAETFRPTRFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 463

BLAST of Bhi03G000666 vs. NCBI nr
Match: XP_004134878.1 (cytochrome P450 84A1 [Cucumis sativus] >KGN48892.1 hypothetical protein Csa_003689 [Cucumis sativus])

HSP 1 Score: 778.1 bits (2008), Expect = 4.3e-221
Identity = 392/467 (83.94%), Postives = 426/467 (91.22%), Query Frame = 0

Query: 1   MDSQYSLLQPLNMLFFFTLPLLFLLTLL-FPPFRRKLPLPPGPKGLPFIGNMLMMDQLTH 60
           MDSQ SLLQPLNM  FFTLP L LLTLL F   R KLP PPGPKGLPFIGNMLM+DQLTH
Sbjct: 1   MDSQ-SLLQPLNMAVFFTLPFLVLLTLLIFRRLRPKLPFPPGPKGLPFIGNMLMLDQLTH 60

Query: 61  RGLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRA 120
           RGLA+LAARYGGIF+MRMGFL+M TV++PDIARQVLQVHD ICSNRPATIAI+YLTY+RA
Sbjct: 61  RGLAKLAARYGGIFYMRMGFLNMFTVADPDIARQVLQVHDSICSNRPATIAISYLTYSRA 120

Query: 121 DMAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVF 180
           DMAFA+YGPFWRQMRK+CVMKLFSRKRAESW+SVRD VDNT+ KVAV+TGTAVN+GELVF
Sbjct: 121 DMAFANYGPFWRQMRKICVMKLFSRKRAESWQSVRDVVDNTVKKVAVQTGTAVNVGELVF 180

Query: 181 ELTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVK 240
           ELTRDIIYRAAFGTS  QGQDEFI ILQEFS+LFGAFNI DFIP L WIDPQGLN RL++
Sbjct: 181 ELTRDIIYRAAFGTSTLQGQDEFISILQEFSKLFGAFNIADFIPGLRWIDPQGLNKRLIE 240

Query: 241 ARRSLDKFIDSIIDDHTNRKK-TTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSED 300
           AR+SLDKFIDSIID+H  +K+  +SKL NY    E +SDMVDELLAFYS+DS+  N S D
Sbjct: 241 ARQSLDKFIDSIIDEHIMKKRNASSKLSNY--KLETESDMVDELLAFYSDDSSIINESND 300

Query: 301 LQNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLER 360
           +QNN KLNRDNIK IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQEL+QVVGL+R
Sbjct: 301 IQNNIKLNRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQR 360

Query: 361 RVEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGR 420
           RVEE+D +NLTF KCCLKET+RLHPPIPLLLHE AEDAV+AGYFIPAKSR+MVNAWAIGR
Sbjct: 361 RVEETDLDNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGR 420

Query: 421 DPASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           DPASWDDAETFRPARFLE+GVPDFKGNNFEFIPFGSGRRSCPGMQLG
Sbjct: 421 DPASWDDAETFRPARFLEEGVPDFKGNNFEFIPFGSGRRSCPGMQLG 464

BLAST of Bhi03G000666 vs. NCBI nr
Match: XP_022962687.1 (cytochrome P450 84A1 [Cucurbita moschata])

HSP 1 Score: 740.3 bits (1910), Expect = 9.9e-210
Identity = 371/453 (81.90%), Postives = 402/453 (88.74%), Query Frame = 0

Query: 13  MLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGI 72
           M   FT+PLLFLL L+F   RRKLP PPGP+GLPF+GNMLMMDQLTHRGLA+LA RYGGI
Sbjct: 1   MALIFTIPLLFLLALIF-WLRRKLPYPPGPRGLPFLGNMLMMDQLTHRGLAKLAKRYGGI 60

Query: 73  FHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQ 132
           FHMRMGFLHMVT+S+PD+ARQVLQVHD ICSNRP TIAI+YLTYN+ADMAFAHYGPFWRQ
Sbjct: 61  FHMRMGFLHMVTISDPDMARQVLQVHDNICSNRPVTIAISYLTYNQADMAFAHYGPFWRQ 120

Query: 133 MRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDIIYRAAFG 192
           MRKLCVMKLFSRKRAESW SVRDEVD+T+  VA + GT VN+GE VFELT +IIYRAAFG
Sbjct: 121 MRKLCVMKLFSRKRAESWASVRDEVDSTVRHVATQLGTVVNVGERVFELTMNIIYRAAFG 180

Query: 193 TSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKARRSLDKFIDSII 252
           TS  QGQDEFI+ILQEFS+LFGAFNI DFIP L WIDPQGLNNRLVKAR+SLDKFID+II
Sbjct: 181 TSTVQGQDEFIKILQEFSKLFGAFNIADFIPNLSWIDPQGLNNRLVKARQSLDKFIDTII 240

Query: 253 DDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIKA 312
           D+H   KK   KL N VKD EI++DMVDEL+AFY E   + N  +D QN  KLNRDNIKA
Sbjct: 241 DEHM-EKKRIRKLSN-VKDDEIETDMVDELVAFYDE-QVSVNELDDSQNGIKLNRDNIKA 300

Query: 313 IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFFK 372
           IIMDVMFGGTETVAS IEW +SELMRSP+DLKKAQQELA VVGL RRVEESD ENL F K
Sbjct: 301 IIMDVMFGGTETVASVIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDMENLAFLK 360

Query: 373 CCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPA 432
           CC+KETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRV+VNAWAIGRDPASWDDAETFRPA
Sbjct: 361 CCIKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPA 420

Query: 433 RFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           RFL+QGV DFKG+NFEFIPFGSGRRSCPGMQLG
Sbjct: 421 RFLKQGVADFKGSNFEFIPFGSGRRSCPGMQLG 449

BLAST of Bhi03G000666 vs. NCBI nr
Match: XP_023003875.1 (cytochrome P450 84A1 [Cucurbita maxima])

HSP 1 Score: 736.9 bits (1901), Expect = 1.1e-208
Identity = 368/453 (81.24%), Postives = 402/453 (88.74%), Query Frame = 0

Query: 13  MLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGI 72
           M   FTLPLLFLL L+F   RRKLP PPGPKGLPF+GNMLMMDQLTHRGLA+LA RYGGI
Sbjct: 1   MALIFTLPLLFLLALIF-RLRRKLPYPPGPKGLPFLGNMLMMDQLTHRGLAKLAKRYGGI 60

Query: 73  FHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQ 132
           FHMRMGFLHMVT+SNPD+ARQVLQV+D +CSNRP TIAI+YLTY++ADMAFAHYGPFWRQ
Sbjct: 61  FHMRMGFLHMVTISNPDMARQVLQVNDNVCSNRPVTIAISYLTYDQADMAFAHYGPFWRQ 120

Query: 133 MRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDIIYRAAFG 192
           MRKLCVMKLFSRKRAESW SVRDE+D+T+  VA + GT VN+GE VFELT +IIYRAAFG
Sbjct: 121 MRKLCVMKLFSRKRAESWASVRDEIDSTVRHVAAQLGTVVNVGERVFELTMNIIYRAAFG 180

Query: 193 TSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKARRSLDKFIDSII 252
           TS  QGQDEFI+ILQEFS+LFGAFNI DF+P L WIDPQGLNNRLVKAR+SLDKFID+II
Sbjct: 181 TSTVQGQDEFIKILQEFSKLFGAFNIADFMPNLSWIDPQGLNNRLVKARQSLDKFIDTII 240

Query: 253 DDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIKA 312
           D+H   KK   KL N VKD +I++DMVDELLAFY E   + N  +D QN  KLNRDNIKA
Sbjct: 241 DEHM-EKKRIRKLSN-VKDDKIETDMVDELLAFYDE-QVSVNELDDSQNGIKLNRDNIKA 300

Query: 313 IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFFK 372
           IIMDVMFGGTETVASAIEW +SELMRSP+DLKKAQQELA VVGL RRVEESD ENL F K
Sbjct: 301 IIMDVMFGGTETVASAIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDLENLAFLK 360

Query: 373 CCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPA 432
           CC+KETLRLHPPIPLLLHETAED VVAGYFIPAKSRV+VNAWAIGRDPASWDDAETFRP+
Sbjct: 361 CCIKETLRLHPPIPLLLHETAEDVVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPS 420

Query: 433 RFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           RFL+QGV DFKG+NFEFIPFGSGRRSCPGMQLG
Sbjct: 421 RFLKQGVADFKGSNFEFIPFGSGRRSCPGMQLG 449

BLAST of Bhi03G000666 vs. ExPASy TrEMBL
Match: A0A5A7SJ62 (Cytochrome P450 84A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001510 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.9e-223
Identity = 396/466 (84.98%), Postives = 422/466 (90.56%), Query Frame = 0

Query: 1   MDSQYSLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHR 60
           MDSQ SLLQPLNM   FTL  LFLLTLLF   R KLP PPGPKGLPFIGNMLMM+QLTHR
Sbjct: 1   MDSQ-SLLQPLNMAVPFTLSFLFLLTLLFRRLRSKLPFPPGPKGLPFIGNMLMMEQLTHR 60

Query: 61  GLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRAD 120
           GLA LAARYGGIF+MRMGF++M  VSNPDIARQVLQVHD ICSNRPATIAI+YLTYN AD
Sbjct: 61  GLANLAARYGGIFYMRMGFVNMFVVSNPDIARQVLQVHDSICSNRPATIAISYLTYNCAD 120

Query: 121 MAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFE 180
           MAFA+YGPFWRQMRK+CVMKLFSRKRAESW+SVRD VDNT+ KVAV+TGTAVNIGELVFE
Sbjct: 121 MAFANYGPFWRQMRKICVMKLFSRKRAESWQSVRDMVDNTVKKVAVQTGTAVNIGELVFE 180

Query: 181 LTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKA 240
           L  DIIYRAAFGTS  QGQDEFIRILQEFS+LFGAFNI DFIP L WIDPQGLN RLVKA
Sbjct: 181 LMGDIIYRAAFGTSTLQGQDEFIRILQEFSKLFGAFNIADFIPSLRWIDPQGLNKRLVKA 240

Query: 241 RRSLDKFIDSIIDDHTNRKK-TTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDL 300
           R+SLDKFIDSIIDDH  RK+  +SKLPNY  + E +SDMVDELLAFY EDS+  N S+DL
Sbjct: 241 RQSLDKFIDSIIDDHIIRKRNASSKLPNY--ELETESDMVDELLAFYGEDSSIRNASDDL 300

Query: 301 QNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERR 360
           +NN KLNRDNIK IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQEL+QVVGL+RR
Sbjct: 301 KNNIKLNRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQRR 360

Query: 361 VEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRD 420
           VEE+D +NLTF KCCLKET+RLHPPIPLLLHE AEDAV+AGYFIPAKSR+MVNAWAIGRD
Sbjct: 361 VEETDLDNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGRD 420

Query: 421 PASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           PASWDDAETFRP RFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG
Sbjct: 421 PASWDDAETFRPTRFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 463

BLAST of Bhi03G000666 vs. ExPASy TrEMBL
Match: A0A1S3B201 (cytochrome P450 84A1-like OS=Cucumis melo OX=3656 GN=LOC103485107 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.9e-223
Identity = 396/466 (84.98%), Postives = 422/466 (90.56%), Query Frame = 0

Query: 1   MDSQYSLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHR 60
           MDSQ SLLQPLNM   FTL  LFLLTLLF   R KLP PPGPKGLPFIGNMLMM+QLTHR
Sbjct: 1   MDSQ-SLLQPLNMAVPFTLSFLFLLTLLFRRLRSKLPFPPGPKGLPFIGNMLMMEQLTHR 60

Query: 61  GLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRAD 120
           GLA LAARYGGIF+MRMGF++M  VSNPDIARQVLQVHD ICSNRPATIAI+YLTYN AD
Sbjct: 61  GLANLAARYGGIFYMRMGFVNMFVVSNPDIARQVLQVHDSICSNRPATIAISYLTYNCAD 120

Query: 121 MAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFE 180
           MAFA+YGPFWRQMRK+CVMKLFSRKRAESW+SVRD VDNT+ KVAV+TGTAVNIGELVFE
Sbjct: 121 MAFANYGPFWRQMRKICVMKLFSRKRAESWQSVRDMVDNTVKKVAVQTGTAVNIGELVFE 180

Query: 181 LTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKA 240
           L  DIIYRAAFGTS  QGQDEFIRILQEFS+LFGAFNI DFIP L WIDPQGLN RLVKA
Sbjct: 181 LMGDIIYRAAFGTSTLQGQDEFIRILQEFSKLFGAFNIADFIPSLRWIDPQGLNKRLVKA 240

Query: 241 RRSLDKFIDSIIDDHTNRKK-TTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDL 300
           R+SLDKFIDSIIDDH  RK+  +SKLPNY  + E +SDMVDELLAFY EDS+  N S+DL
Sbjct: 241 RQSLDKFIDSIIDDHIIRKRNASSKLPNY--ELETESDMVDELLAFYGEDSSIRNASDDL 300

Query: 301 QNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERR 360
           +NN KLNRDNIK IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQEL+QVVGL+RR
Sbjct: 301 KNNIKLNRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQRR 360

Query: 361 VEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRD 420
           VEE+D +NLTF KCCLKET+RLHPPIPLLLHE AEDAV+AGYFIPAKSR+MVNAWAIGRD
Sbjct: 361 VEETDLDNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGRD 420

Query: 421 PASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           PASWDDAETFRP RFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG
Sbjct: 421 PASWDDAETFRPTRFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 463

BLAST of Bhi03G000666 vs. ExPASy TrEMBL
Match: A0A0A0KKL4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504720 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.1e-221
Identity = 392/467 (83.94%), Postives = 426/467 (91.22%), Query Frame = 0

Query: 1   MDSQYSLLQPLNMLFFFTLPLLFLLTLL-FPPFRRKLPLPPGPKGLPFIGNMLMMDQLTH 60
           MDSQ SLLQPLNM  FFTLP L LLTLL F   R KLP PPGPKGLPFIGNMLM+DQLTH
Sbjct: 1   MDSQ-SLLQPLNMAVFFTLPFLVLLTLLIFRRLRPKLPFPPGPKGLPFIGNMLMLDQLTH 60

Query: 61  RGLARLAARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRA 120
           RGLA+LAARYGGIF+MRMGFL+M TV++PDIARQVLQVHD ICSNRPATIAI+YLTY+RA
Sbjct: 61  RGLAKLAARYGGIFYMRMGFLNMFTVADPDIARQVLQVHDSICSNRPATIAISYLTYSRA 120

Query: 121 DMAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVF 180
           DMAFA+YGPFWRQMRK+CVMKLFSRKRAESW+SVRD VDNT+ KVAV+TGTAVN+GELVF
Sbjct: 121 DMAFANYGPFWRQMRKICVMKLFSRKRAESWQSVRDVVDNTVKKVAVQTGTAVNVGELVF 180

Query: 181 ELTRDIIYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVK 240
           ELTRDIIYRAAFGTS  QGQDEFI ILQEFS+LFGAFNI DFIP L WIDPQGLN RL++
Sbjct: 181 ELTRDIIYRAAFGTSTLQGQDEFISILQEFSKLFGAFNIADFIPGLRWIDPQGLNKRLIE 240

Query: 241 ARRSLDKFIDSIIDDHTNRKK-TTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSED 300
           AR+SLDKFIDSIID+H  +K+  +SKL NY    E +SDMVDELLAFYS+DS+  N S D
Sbjct: 241 ARQSLDKFIDSIIDEHIMKKRNASSKLSNY--KLETESDMVDELLAFYSDDSSIINESND 300

Query: 301 LQNNFKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLER 360
           +QNN KLNRDNIK IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQEL+QVVGL+R
Sbjct: 301 IQNNIKLNRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQR 360

Query: 361 RVEESDFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGR 420
           RVEE+D +NLTF KCCLKET+RLHPPIPLLLHE AEDAV+AGYFIPAKSR+MVNAWAIGR
Sbjct: 361 RVEETDLDNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGR 420

Query: 421 DPASWDDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           DPASWDDAETFRPARFLE+GVPDFKGNNFEFIPFGSGRRSCPGMQLG
Sbjct: 421 DPASWDDAETFRPARFLEEGVPDFKGNNFEFIPFGSGRRSCPGMQLG 464

BLAST of Bhi03G000666 vs. ExPASy TrEMBL
Match: A0A6J1HDA0 (cytochrome P450 84A1 OS=Cucurbita moschata OX=3662 GN=LOC111463105 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 4.8e-210
Identity = 371/453 (81.90%), Postives = 402/453 (88.74%), Query Frame = 0

Query: 13  MLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGI 72
           M   FT+PLLFLL L+F   RRKLP PPGP+GLPF+GNMLMMDQLTHRGLA+LA RYGGI
Sbjct: 1   MALIFTIPLLFLLALIF-WLRRKLPYPPGPRGLPFLGNMLMMDQLTHRGLAKLAKRYGGI 60

Query: 73  FHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQ 132
           FHMRMGFLHMVT+S+PD+ARQVLQVHD ICSNRP TIAI+YLTYN+ADMAFAHYGPFWRQ
Sbjct: 61  FHMRMGFLHMVTISDPDMARQVLQVHDNICSNRPVTIAISYLTYNQADMAFAHYGPFWRQ 120

Query: 133 MRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDIIYRAAFG 192
           MRKLCVMKLFSRKRAESW SVRDEVD+T+  VA + GT VN+GE VFELT +IIYRAAFG
Sbjct: 121 MRKLCVMKLFSRKRAESWASVRDEVDSTVRHVATQLGTVVNVGERVFELTMNIIYRAAFG 180

Query: 193 TSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKARRSLDKFIDSII 252
           TS  QGQDEFI+ILQEFS+LFGAFNI DFIP L WIDPQGLNNRLVKAR+SLDKFID+II
Sbjct: 181 TSTVQGQDEFIKILQEFSKLFGAFNIADFIPNLSWIDPQGLNNRLVKARQSLDKFIDTII 240

Query: 253 DDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIKA 312
           D+H   KK   KL N VKD EI++DMVDEL+AFY E   + N  +D QN  KLNRDNIKA
Sbjct: 241 DEHM-EKKRIRKLSN-VKDDEIETDMVDELVAFYDE-QVSVNELDDSQNGIKLNRDNIKA 300

Query: 313 IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFFK 372
           IIMDVMFGGTETVAS IEW +SELMRSP+DLKKAQQELA VVGL RRVEESD ENL F K
Sbjct: 301 IIMDVMFGGTETVASVIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDMENLAFLK 360

Query: 373 CCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPA 432
           CC+KETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRV+VNAWAIGRDPASWDDAETFRPA
Sbjct: 361 CCIKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPA 420

Query: 433 RFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           RFL+QGV DFKG+NFEFIPFGSGRRSCPGMQLG
Sbjct: 421 RFLKQGVADFKGSNFEFIPFGSGRRSCPGMQLG 449

BLAST of Bhi03G000666 vs. ExPASy TrEMBL
Match: A0A6J1KT07 (cytochrome P450 84A1 OS=Cucurbita maxima OX=3661 GN=LOC111497337 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.3e-209
Identity = 368/453 (81.24%), Postives = 402/453 (88.74%), Query Frame = 0

Query: 13  MLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARLAARYGGI 72
           M   FTLPLLFLL L+F   RRKLP PPGPKGLPF+GNMLMMDQLTHRGLA+LA RYGGI
Sbjct: 1   MALIFTLPLLFLLALIF-RLRRKLPYPPGPKGLPFLGNMLMMDQLTHRGLAKLAKRYGGI 60

Query: 73  FHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAHYGPFWRQ 132
           FHMRMGFLHMVT+SNPD+ARQVLQV+D +CSNRP TIAI+YLTY++ADMAFAHYGPFWRQ
Sbjct: 61  FHMRMGFLHMVTISNPDMARQVLQVNDNVCSNRPVTIAISYLTYDQADMAFAHYGPFWRQ 120

Query: 133 MRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDIIYRAAFG 192
           MRKLCVMKLFSRKRAESW SVRDE+D+T+  VA + GT VN+GE VFELT +IIYRAAFG
Sbjct: 121 MRKLCVMKLFSRKRAESWASVRDEIDSTVRHVAAQLGTVVNVGERVFELTMNIIYRAAFG 180

Query: 193 TSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKARRSLDKFIDSII 252
           TS  QGQDEFI+ILQEFS+LFGAFNI DF+P L WIDPQGLNNRLVKAR+SLDKFID+II
Sbjct: 181 TSTVQGQDEFIKILQEFSKLFGAFNIADFMPNLSWIDPQGLNNRLVKARQSLDKFIDTII 240

Query: 253 DDHTNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNFKLNRDNIKA 312
           D+H   KK   KL N VKD +I++DMVDELLAFY E   + N  +D QN  KLNRDNIKA
Sbjct: 241 DEHM-EKKRIRKLSN-VKDDKIETDMVDELLAFYDE-QVSVNELDDSQNGIKLNRDNIKA 300

Query: 313 IIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEESDFENLTFFK 372
           IIMDVMFGGTETVASAIEW +SELMRSP+DLKKAQQELA VVGL RRVEESD ENL F K
Sbjct: 301 IIMDVMFGGTETVASAIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDLENLAFLK 360

Query: 373 CCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPA 432
           CC+KETLRLHPPIPLLLHETAED VVAGYFIPAKSRV+VNAWAIGRDPASWDDAETFRP+
Sbjct: 361 CCIKETLRLHPPIPLLLHETAEDVVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPS 420

Query: 433 RFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLG 466
           RFL+QGV DFKG+NFEFIPFGSGRRSCPGMQLG
Sbjct: 421 RFLKQGVADFKGSNFEFIPFGSGRRSCPGMQLG 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G36220.11.1e-18769.68ferulic acid 5-hydroxylase 1 [more]
AT5G04330.14.5e-15259.83Cytochrome P450 superfamily protein [more]
AT3G26310.16.8e-8438.38cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT3G48270.14.4e-8336.17cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT5G07990.19.9e-8337.99Cytochrome P450 superfamily protein [more]
Match NameE-valueIdentityDescription
Q426001.6e-18669.68Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1[more]
F4JW836.3e-15159.83Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1[more]
P244653.2e-9441.47Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A0N9HTU16.9e-8940.00Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 ... [more]
A6YIH83.8e-8740.09Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883545.16.1e-268100.00cytochrome P450 84A1-like [Benincasa hispida][more]
XP_008440792.11.0e-22284.98PREDICTED: cytochrome P450 84A1-like [Cucumis melo] >KAA0025694.1 cytochrome P45... [more]
XP_004134878.14.3e-22183.94cytochrome P450 84A1 [Cucumis sativus] >KGN48892.1 hypothetical protein Csa_0036... [more]
XP_022962687.19.9e-21081.90cytochrome P450 84A1 [Cucurbita moschata][more]
XP_023003875.11.1e-20881.24cytochrome P450 84A1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5A7SJ624.9e-22384.98Cytochrome P450 84A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3B2014.9e-22384.98cytochrome P450 84A1-like OS=Cucumis melo OX=3656 GN=LOC103485107 PE=3 SV=1[more]
A0A0A0KKL42.1e-22183.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504720 PE=3 SV=1[more]
A0A6J1HDA04.8e-21081.90cytochrome P450 84A1 OS=Cucurbita moschata OX=3662 GN=LOC111463105 PE=3 SV=1[more]
A0A6J1KT075.3e-20981.24cytochrome P450 84A1 OS=Cucurbita maxima OX=3661 GN=LOC111497337 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 459..465
score: 24.94
coord: 320..337
score: 32.13
coord: 373..384
score: 40.05
coord: 450..459
score: 58.92
IPR001128Cytochrome P450PFAMPF00067p450coord: 39..464
e-value: 6.2E-90
score: 302.1
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 309..326
score: 31.13
coord: 412..436
score: 36.1
coord: 449..459
score: 57.93
coord: 459..465
score: 13.09
coord: 329..355
score: 34.27
coord: 66..85
score: 28.62
coord: 372..390
score: 29.01
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 32..465
e-value: 1.5E-113
score: 382.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 32..465
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 22..462
e-value: 3.9E-9
score: 33.7
NoneNo IPR availablePANTHERPTHR47945:SF4CYTOCHROME P450 FAMILY FERULATE HYDROXYLASEcoord: 11..465
NoneNo IPR availablePANTHERPTHR47945CYTOCHROME P450 84A1-RELATEDcoord: 11..465
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 452..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000666Bhi03M000666mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009809 lignin biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen